Highlights Improving spikelet production efficiency(SPE)can further enhance the grain yield and harvest index in rice.Higher SPE can enhance post-anthesis photoassimilate production in the shoots of rice.Higher SPE ca...Highlights Improving spikelet production efficiency(SPE)can further enhance the grain yield and harvest index in rice.Higher SPE can enhance post-anthesis photoassimilate production in the shoots of rice.Higher SPE can promote the post-anthesis remobilization of non-structural carbohydrate(NSC)from rice stems.Rice grain yield is a complex agronomic trait determined by four key components:panicles per plant,spikelets per panicle,filled-grain rate,and grain weight.展开更多
Intensive agricultural practices have stimulated progressive degradation of soil quality in the Mollisols of Northeast China,resulting in curtailed crop productivity.Although conservation tillage patterns have been mo...Intensive agricultural practices have stimulated progressive degradation of soil quality in the Mollisols of Northeast China,resulting in curtailed crop productivity.Although conservation tillage patterns have been modified to better suit Mollisols in recent years,whether conservation tillage can effectively enhance soil organic carbon(SOC)and crop yield remains controversial.In our study,the effects of two modified conservation tillage(MCT)patterns,the strip tillage with straw cover(STSC)and straw mixture amendment tillage(SMAT),on soil aggregate stability,SOC stock,enzyme activities,microbial communities,and crop yields were evaluated after five years of implementation.The results revealed that compared with conventional tillage(CT),both STSC and SMAT increased soil dissolved organic carbon(DOC),easily oxidized organic carbon(EOC),particulate organic carbon(POC),and mineral-bound organic carbon(MOC),thereby raising SOC content and reinforcing the stability of soil aggregates.The microbial taxa,Bacillus,Gemmatimonadaceae,Sphingomonas,Nocardioides,Blastococcus,Streptomyces,Trichoderma,Holtermanniella,Talaromyces,Didymella,and Fusicolla,were identified as potential contributors to carbon(C)sequestration.Soil microbial diversity and key taxa were positively correlated with SOC.Additionally,both STSC and SMAT raised the effective panicle number,kernels per ear,and 100-grain weight,as well as nitrogen and phosphorus absorption by maize grains and rhizomes,further increasing maize yield,with a more pronounced effect of SMAT than STSC.To sum up,the implementation of MCT can considerably strengthen C sequestration and crop productivity in the Mollisol region of Northeast China,contributing to farmland ecological protection and improved agroecological efficiency.展开更多
Precise manipulation of complex agronomic traits often requires the simultaneous introduction of mutations at multiple gene loci,a capability that remains challenging for existing genome-editing technologies.In this s...Precise manipulation of complex agronomic traits often requires the simultaneous introduction of mutations at multiple gene loci,a capability that remains challenging for existing genome-editing technologies.In this study,we developed and systematically compared five strategies for concurrent knockout and point mutation induction(KAPSi)based on prime editing,aiming to integrate precise nucleotide substitutions with efficient gene knockout within a single editing framework.展开更多
Phytolacca americana is a known rare earth element(REE)hyperaccumulator,notable for its high biomass and rapid growth rate.However,its capacity to tolerate and accumulate REEs under high-exposure conditions is relativ...Phytolacca americana is a known rare earth element(REE)hyperaccumulator,notable for its high biomass and rapid growth rate.However,its capacity to tolerate and accumulate REEs under high-exposure conditions is relatively limited,which restricts its utilization in agromining on soils heavily contaminated with REEs.Silicon(Si)has been shown to effectively mitigate metal(loid)s stress in various plants,and to promote REE tolerance in the well-known hyperaccumulator fern Dicranopteris linearis.This study aimed to assess the effects of Si on REE agromining using P.americana and to elucidate the underlying mechanisms.The results show that foliar Si application significantly increases the REEs accumulation of P.americana by as much as 73.6%and 172.4%in hydroponic and pot experiments respectively.Notably,Si application enhances the retention of REEs within the cell walls,primarily through the formation of silicate particles and/or modifications of the cell walls.Transcriptome analysis indicates that foliar Si application initially mitigates the toxicity of REEs by inducing an upregulation of genes associated with stress response and antioxidant enzymes,which facilitates the rapid elimination of accumulated reactive oxygen species.With increasing Si application,the abnormal overexpression of stress response genes,as well as genes involved in flavonoid biosynthesis and cell division,is alleviated,further implying that the entry of REE into the cytoplasm can be effectively hindered.This study shows that foliar Si application represents a potentially cost-efficiency agronomic practice for improvement of REE agromining using P.americana.展开更多
Introduction:technical bottlenecks in genomic research on sugarcane The improvement of sugarcane(Saccharum spp.)is severely hindered by complex mixed ploidy,aneuploidy,and interspecific hybridization.Recently,Huang et...Introduction:technical bottlenecks in genomic research on sugarcane The improvement of sugarcane(Saccharum spp.)is severely hindered by complex mixed ploidy,aneuploidy,and interspecific hybridization.Recently,Huang et al.constructed the first multiscale sugarcane pangenome graph integrating nine assemblies[1].Overcoming traditional linear reference limitations,this framework increased genomic diversity capture from 34%to 82%and identified key agronomic loci(e.g.,sugar content,leaf angle)via the novel dosage genome-wide association study(GWAS)method.We highlight this study's technical,statistical,and biological dimensions.展开更多
This study aimed to elucidate the effects of varying seedling ages at planting on the agronomic traits and nutrient content of stem lettuce.The early-maturing variety“WS120”and the late-maturing variety“WS1”were e...This study aimed to elucidate the effects of varying seedling ages at planting on the agronomic traits and nutrient content of stem lettuce.The early-maturing variety“WS120”and the late-maturing variety“WS1”were employed as experimental materials.Four seedling age treatments were established at 20,25,30,and 35 d.By measuring the agronomic traits and nutrient content of the stem lettuce,we employed correlation analysis,principal component analysis,cluster analysis,and the membership function method for a comprehensive evaluation.This study aims to elucidate the optimal planting age for stem lettuce in plateau regions.By addressing the issues of inconsistent quality and yield that arise from arbitrary selection of planting age in production,we seek to establish a theoretical foundation for the development of a high-quality and efficient cultivation technology system for this crop.The results of the correlation analysis revealed that a total of 18 pairs of indicators in WS1 exhibited significant correlations,comprising 8 pairs that were positively correlated and 10 pairs that were negatively correlated.In WS120,25 pairs of indicators reached a significant correlation level,with 13 pairs positively correlated and 12 pairs negatively correlated.Principal component analysis identified 14 agronomic traits and 4 quality indicators,which were consolidated into 3 principal components,achieving cumulative contribution rates of 94.068%.Cluster analysis results indicated that WS1 could be categorised into two groups based on different treatments.At 30 d and 35 d,the samples were grouped together due to superior root-related indicators and nutritional components,whereas at 25 d and 20 d,they formed a separate group owing to enhanced agronomic traits.WS120 was classified into Group I,reflecting relatively favourable agronomic traits at 25 d and 20 d.Group II(30 d)was characterised by all indicators falling within the mid-range.Class III(35 d)was distinctly categorised due to elevated levels of vitamin C,soluble sugars,and soluble proteins.A comprehensive evaluation employing the membership function method indicated that the overall performance of WS1 and WS120 under varying treatments was ranked as follows:25 d>30 d>20 d>35 d.It can be utilised directly to inform production practices and holds considerable practical importance for advancing the high-quality and efficient cultivation of stem lettuce,thereby contributing to the growth of the plateau vegetable industry.展开更多
Jujube(Ziziphus jujuba Mill.)is a fruit crop of high economic value,renowned for its distinctive flavor and wide range of phenotypic diversity.Despite major advancements in jujube genomics,the role of genetic variants...Jujube(Ziziphus jujuba Mill.)is a fruit crop of high economic value,renowned for its distinctive flavor and wide range of phenotypic diversity.Despite major advancements in jujube genomics,the role of genetic variants in underlying agronomic trait formation is still poorly understood.Here,we used seven high-quality jujube genomes to construct a pan-TE(transposon element)map and investigated how TEs shape genome evolution and agronomic traits.We found that TEs constitute 29.05%30.38%of the genome,predominantly long terminal repeat(LTR)retrotransposons such as Copia and Gypsy.A positive correlation(R2=0.76)between TE content and genome size underscores their role in genomic expansion.TE insertions within gene bodies significantly reduce gene expression,particularly for genes involved in cell wall biosynthesis and glucose metabolism.Population scale analysis of 1041 accessions identified 4176 transposable element insertion polymorphisms(TIPs)that distinguish wild and cultivated groups.Wild jujubes harbor stress-related TiPs(e.g.in peroxidase genes),whereas cultivated accessions carry TiPs linked to fruit development.Notably,a Gypsy insertion upstream of the cellulose synthase gene ZjCESA4 is associated with reduced expression and thinner pericarp in'Dongzao'compared to'Huizao'.Similarly,a downstream LTR/Gypsy insertion near the MADS-box transcription factor gene ZjAGL18 correlates with suppressed expression,highlighting the recurrent targeting of key regulatory genes by TEs during domestication.Our findings demonstrate that TiPs are a major source of genetic variation in jujube,providing molecular markers for breeding programs that aim to balance fruit quality and stress resilience.展开更多
Pear(Pyrus L.)is a fruit tree of global commercial importance.Its genetic relationships,evolutionary history,dissemination routes,and genetic determinants ofmost agronomic traits remain to be elucidated.We conducted w...Pear(Pyrus L.)is a fruit tree of global commercial importance.Its genetic relationships,evolutionary history,dissemination routes,and genetic determinants ofmost agronomic traits remain to be elucidated.We conducted whole-genome resequencing of 495 Pyrus accessions.Phylogenetic and demographic analyses resolved geographic groupings of the accessions,identifying the Yunnan-Guizhou Plateau as the putative dissemination center for cultivated Pyrus pyrifolia and P.bretschneideri.Identification of two evolutionary bottlenecks provides insights into the population dynamics of pear species.Admixture and introgression analyses revealed both intraspecific and interspecific genetic exchanges,substantiating the complex emergence of cultivated populations.Genome-wide association study(GWAS)identified loci associated withnine crucial agronomic traits,together with eight candidate genes.The GWAS,molecular,and biochemical analyses suggested that PbeMADS25,PbeSPP,PbeDHQ-SDH,PbeARF2,PbePPO,PbePIN3,PbeCXE,and PbeMYB38 participate in the regulation of number of stigmas and number of locules,number of stamens,young leaf color,sepal persistence,astringency,acidity,aroma,and fruit skin color,respectively.Overexpression and metabonomic analysis of PbeCXE indicated that it affects the fruit aroma by affecting the balance between ester biosynthesis and substrate consumption.These findings expand our understanding of Pyrus evolution and provide a genomic foundation for genetic improvement of agronomic traits.展开更多
[Objectives]To investigate the effects of different planting densities and nitrogen application rates on the yield and quality of the tobacco cultivar Chuxue 80.[Methods]A field experiment was conducted in Hubei Provi...[Objectives]To investigate the effects of different planting densities and nitrogen application rates on the yield and quality of the tobacco cultivar Chuxue 80.[Methods]A field experiment was conducted in Hubei Province,evaluating various combinations of planting density and nitrogen rate for Chuxue 80.[Results]At the maturity stage,the TN1 treatment(5 kg N per 667 m2 with a density of 1900 plants per 667 m2)demonstrated the most favorable agronomic performance.The TN9 treatment(11 kg N per 667 m2 with a density of 1110 plants per 667 m2)achieved the highest wrapper tobacco yield and output value.Meanwhile,the TN5 treatment(8 kg N per 667 m2 with a density of 1515 plants per 667 m2)resulted in the best smoking quality.[Conclusions]The TN9 treatment,with a planting density of 1110 plants per 667 m2 and a nitrogen application rate of 11 kg per 667 m2,is recommended as the optimal cultivation practice for Chuxue 80 in Hubei Province.展开更多
Medicinal plants are foundational to global health systems,yet their increasing integration into modern pharmaceutical supply chains has intensified sustainability challenges associated with climate change,biodiversit...Medicinal plants are foundational to global health systems,yet their increasing integration into modern pharmaceutical supply chains has intensified sustainability challenges associated with climate change,biodiversity loss,and market volatility.Artemisia annua L.,the sole commercial source of artemisinin—the cornerstone of artemisinin-based combination therapies for malaria—exemplifies these tensions.Despite decades of agronomic and biotechnological research,global artemisinin supply remains unstable,largely due to yield variability,monoculture-driven ecological risks,and socio-economic vulnerabilities faced by smallholder producers.This literature-based review synthesizes advances across agronomy,climate-adaptive breeding,artificial intelligence–enabled precision agriculture,and socio-economic governance to propose an integrated framework for the sustainable expansion of A.annua cultivation.Central to the framework is the repositioning of agrobiodiversity—from a perceived constraint on productivity to a primary mechanism for enhancing ecological resilience,yield stability,and farmer livelihoods.The framework emphasizes biodiversity-centric agronomic practices,multi-latitude phenological adaptation,inclusive intelligent farming systems,and Public–Private–Farmer Partnerships(PPFPs)designed to promote equitable value distribution.While the framework is conceptual in nature,it is grounded in empirical evidence from diverse disciplinary domains.To facilitate translation into practice,a structured roadmap for empirical validation is proposed,outlining multi-location,multi-year field trials integrating agronomic,ecological,and socioeconomic metrics.Collectively,this work provides a systems-level blueprint for stabilizing the global artemisinin supply while aligning medicinal plant production with biodiversity conservation,climate resilience,and social equity objectives.展开更多
To enhance soil fertility in Chinese orchards, this study conducts comprehensive research and applies specialized agricultural techniques. Addressing prevalent issues such as insufficient organic matter, rigid soil st...To enhance soil fertility in Chinese orchards, this study conducts comprehensive research and applies specialized agricultural techniques. Addressing prevalent issues such as insufficient organic matter, rigid soil structure, nutrient imbalance, and declining biological activity, the paper thoroughly analyzes soil degradation patterns and their impacts on fruit yield and quality. The research framework comprises four sections: 1) Detailed implementation procedures for increasing soil organic content and improving physical properties through scientific approaches—including organic fertilizer application, green plant cultivation, surface grass coverage, and soil mulching; 2) Precise fertilization methods based on soil testing, micronutrient supplementation, and efficient irrigation systems combining drip and sprinkler irrigation; 3) Practical strategies utilizing functional microbial agents and treated carbon materials to optimize soil environment, promoting intercropping to boost microbial diversity, and maintaining ecosystem stability; 4) Integrated optimization of these techniques into a ready-to-implement agricultural solution validated through field trials, demonstrating its effectiveness in enhancing soil fertility, promoting healthy tree growth, and boosting economic returns. The objective is to establish a cost-effective, eco-friendly, and sustainable soil fertility improvement system tailored for long-term orchard applications, providing robust theoretical and technical support for sustained high-yield production.展开更多
To address the rising global agricultural labor costs,there is an urgent need for robots to accomplish some complex agronomic tasks and break through the limitations of traditional machinery.Thus,robots are considered...To address the rising global agricultural labor costs,there is an urgent need for robots to accomplish some complex agronomic tasks and break through the limitations of traditional machinery.Thus,robots are considered an essential support for the future smart agriculture.Given that agronomic targets,such as plants and animals,are living organisms with diverse growth patterns and physical characteristics,effective hand–eye coordination is crucial for robots to interact with these targets proficiently.This paper reviews the developments in hand–eye coordination technology for agricultural robots,focusing on its configuration,principles,and applications in target detection and manipulation,based on a review of research literature and technical specifications of commercial products.Furthermore,the ongoing challenges in hand–eye coordination for robotic operations in complex agronomic tasks are analyzed and summarized,and the potential trends for overcoming these challenges are predicted.Finally,this review aims to deepen understanding of agricultural robots’capabilities and inspire ongoing innovation to further their agricultural applications.展开更多
Powdery mildew is a serious disease caused by Blumeria graminis f.sp.tritici(Bgt)that critically threatens the yield and quality of wheat(Triticum aestivum L.).Using effective powdery mildew resistance genes is the op...Powdery mildew is a serious disease caused by Blumeria graminis f.sp.tritici(Bgt)that critically threatens the yield and quality of wheat(Triticum aestivum L.).Using effective powdery mildew resistance genes is the optimal method for controlling this disease.Against the background of high genetic homogeneity among the modern commercial cultivars that are mainly derived from conventional interbreeding,the resistance genes from wheat relatives have especially prominent advantages.Octoploid triticale,produced from common wheat and rye(Secale cereale L.)through distant hybridization,is a new synthetic species and valuable gene donor for wheat improvement.In this study,we developed the wheat-rye line YT5 through the hybridization of octaploid triticale and two wheat lines.YT5 was confirmed to be a 6RL ditelosomic addition and 1R(1B)substitution line using genomic in situ hybridization(GISH),multicolor fluorescence in situ hybridization(mc-FISH),multicolor GISH(mc-GISH)and molecular marker analysis.Genetic analysis showed that the powdery mildew resistance in YT5 was derived from the rye chromosome arm 6RL.After inoculation with different Bgt isolates at the seedling stage,YT5 had compound reaction patterns with both obvious spores and hypersensitivity,and it gradually became highly resistant until the adult-plant stage,thus showing a resistance response significantly different from the reported Pm genes from rye chromosome 6RL.YT5 also showed promising agronomic performance,so it is expected to be an elite resistance donor for wheat improvement.To promote the transfer of the chromosome arm 6RL of YT5 in marker-assisted selection(MAS)breeding,we selected and verified two 6RL-specific kompetitive allelespecific PCR(KASP)markers that can be applied to efficiently detect this chromosome arm in different wheat backgrounds.展开更多
THE development of agriculture faces significant challenges due to population growth,climate change,land depletion,and environmental pollution,threatening global food security[1].This necessitates the development of s...THE development of agriculture faces significant challenges due to population growth,climate change,land depletion,and environmental pollution,threatening global food security[1].This necessitates the development of sustainable agriculture,where a fundamental step is crop breeding to improve agronomic or economic traits,e.g.,increasing yields of crops while decreasing resource usage and minimizing pollution to the environment[2].展开更多
Small signaling peptides,generally comprising fewer than 100 amino acids,act as crucial signaling molecules in cell-to-cell communications.Upon perception by their membrane-localized corresponding receptors or co-rece...Small signaling peptides,generally comprising fewer than 100 amino acids,act as crucial signaling molecules in cell-to-cell communications.Upon perception by their membrane-localized corresponding receptors or co-receptors,these peptide-receptor modules then(de)activate either long-distance or local signaling pathways,thereby orchestrating developmental and adaptive responses via(post)transcriptional,(post)translational,and epigenetic regulations.The physiological functions of small signaling peptides are implicated in a multitude of developmental processes and adaptive responses,including but not limited to,shoot and root morphogenesis,organ abscission,nodulation,Casparian strip formation,pollen development,taproot growth,and various abiotic stress responses such as aluminum,cadmium,drought,cold,and salinity.Additionally,they play a critical role in response to pathogenic invasions.These small signaling peptides also modulate significant agronomic and horticultural traits,such as fruit size,maize kernel development,fiber elongation,and rice awn formation.Here,we underscore the roles of several small signaling peptide families such as CLE,RALF,EPFL,mi PEP,CEP,IDA/IDL,and PSK in regulating these biological processes.These novel insights will deepen our current understanding of small signaling peptides,and offer innovative strategies for genetic breeding stress-tolerant crops and horticultural plants,contributing to establish sustainable agricultural systems.展开更多
Epigenetics-mediated breeding(epibreeding)involves engineering crop traits and stress responses through the targeted manipulation of key epigenetic features to enhance agricultural productivity.While conventional bree...Epigenetics-mediated breeding(epibreeding)involves engineering crop traits and stress responses through the targeted manipulation of key epigenetic features to enhance agricultural productivity.While conventional breeding methods raise concerns about reduced genetic diversity,epibreeding propels crop improvement through epigenetic variations that regulate gene expression,ultimately impacting crop yield.Epigenetic regulation in crops encompasses various modes,including histone modification,DNA modification,RNA modification,non-coding RNA,and chromatin remodeling.This review summarizes the epigenetic mechanisms underlying major agronomic traits in maize and identifies candidate epigenetic landmarks in the maize breeding process.We propose a valuable strategy for improving maize yield through epibreeding,combining CRISPR/Cas-based epigenome editing technology and Synthetic Epigenetics(SynEpi).Finally,we discuss the challenges and opportunities associated with maize trait improvement through epibreeding.展开更多
Banana breeding is hampered by the very low fertility of domesticated bananas and the lack of knowledge about the genetic determinism of agronomic traits.We analysed a breeding population of 2723 triploid hybrids resu...Banana breeding is hampered by the very low fertility of domesticated bananas and the lack of knowledge about the genetic determinism of agronomic traits.We analysed a breeding population of 2723 triploid hybrids resulting from crosses between diploid and tetraploid Musa acuminata parents,which was evaluated over three successive crop cycles for 24 traits relating to yield components and plant,bunch,and fruit architectures.A subset of 1129 individuals was genotyped by sequencing,revealing 205612 single-nucleotide polymorphisms(SNPs).Most parents were heterozygous for one or several large reciprocal chromosomal translocations,which are known to impact recombination and chromosomal segregation.We applied two linear mixed models to detect associations between markers and traits:(i)a standard model with a kinship calculated using all SNPs and(ii)a model with chromosome-specific kinships that aims at recovering statistical power at alleles carried by long non-recombined haplotypic segments.For 23 of the 24 traits,we identified one to five significant quantitative trait loci(QTLs)for which the origin of favourable alleles could often be determined amongst the main ancestral contributors to banana cultivars.Several QTLs,located in the rearranged regions,were only detected using the second model.The resulting QTL landscape represents an important resource to support breeding programmes.The proposed strategy for recovering power at SNPs carried by long non-recombined rearranged haplotypic segments is an important methodological advance for future association studies in banana and other species affected by chromosomal rearrangements.展开更多
Alfalfa(Medicago sativa L.),a perennial legume forage,has been broadly cultivated owing to a variety of favorable characteristics,including comprehensive ecological adaptability,superior nutritive value and palatabili...Alfalfa(Medicago sativa L.),a perennial legume forage,has been broadly cultivated owing to a variety of favorable characteristics,including comprehensive ecological adaptability,superior nutritive value and palatability,and nitrogen fixation capacity.The productivity traits of alfalfa,specifically its biomass yield and forage quality,are significantly influenced by a series of determinants,including internal developmental factors and external environmental cues.However,the regulatory mechanisms underlying the fundamental biological problems of alfalfa remain elusive.Here,we conducted a comprehensive review focusing on the genomics of alfalfa,advancements in gene-editing technologies,and the identification of genes that control pivotal agronomic characteristics,including biomass formation,nutritional quality,flowering time,and resistance to various stresses.Moreover,a molecular design roadmap for the‘ideal alfalfa’has been proposed and the potential of pangenomes,self-incompatibility mechanisms,de novo domestication,and intelligent breeding strategies to enhance alfalfa’s yield,quality,and resilience were further discussed.This review will provide comprehensive information on the basic biology of alfalfa and offer new insights for the cultivation of ideal alfalfa.展开更多
Cherries are one of the economically important fruit crops in the Rosaceae family,Prunus genus.As the first fruits of the spring season in the northern hemisphere,their attractive appearance,intensely desirable tastes...Cherries are one of the economically important fruit crops in the Rosaceae family,Prunus genus.As the first fruits of the spring season in the northern hemisphere,their attractive appearance,intensely desirable tastes,high nutrients content,and consumer-friendly size captivate consumers worldwide.In the past 30 years,although cherry geneticists and breeders have greatly progressed in understanding the genetic and molecular basis underlying fruit quality,adaptation to climate change,and biotic and abiotic stress resistance,the utilization of cherry genomic data in genetics and molecular breeding has remained limited to date.Here,we thoroughly investigated recent discoveries in constructing genetic linkage maps,identifying quantitative trait loci(QTLs),genome-wide association studies(GWAs),and validating functional genes of edible cherries based on available de nouo genomes and genome resequencing data of edible cherries.We further comprehensively demonstrated the genetic architecture of the main agronomic traits of edible cherries by methodically integrating QTLs,GWAs loci,and functional genes into the identical reference genome with improved annotations.These collective endeavors will offer new perspectives on the availability of sequence data and the construction of an interspecific pangenome of edible cherries,ultimately guiding cherry breeding strategies and genetic improvement programs,and facilitating the exploration of similar traits and breeding innovations across Prunus species.展开更多
High molecular weight glutenin subunits(HMW-GS),major components of seed storage proteins in wheat,have large effects on processing quality.GLU-1 genes encode HMW-GS and their expression is mainly controlled at the tr...High molecular weight glutenin subunits(HMW-GS),major components of seed storage proteins in wheat,have large effects on processing quality.GLU-1 genes encode HMW-GS and their expression is mainly controlled at the transcriptional level by interactions between cis-regulatory elements and transcription factors.We previously identified an Aux/IAA transcription factor TaIAA10-6D that bound to a conserved cis-regulatory module CCRM1-1,the most essential conserved cis-regulatory module in GLU-1.Here,we confirmed the binding of TaIAA10-6D to CCRM1-1 using yeast one hybrid and dualluciferase reporter assays.The enhanced expression of TaIAA10-6D suppressed glutenin accumulation and increased gliadin content.Dynamic transcriptome analyses revealed that TaIAA10-6D overexpression down-regulated glutenin and gliadin genes during an early stage of grain filling,but up-regulated gliadin genes during a late stage probably by endoplasmic reticulum stress,accounting for its effect on the tradeoff between glutenin and gliadin.Rheological property and processing quality assays showed that TaIAA10-6D overproduction reduced stabilization time and bread quality,but enhanced cookie quality.Overexpression of TaIAA10-6D also reduced plant height,leaf size,kernel number and grain yield.We identified two major haplotypes of TaIAA10-6D,Hap I and Hap II,and developed a breeding-friendly diagnostic marker.Hap I conferred higher expression of TaIAA10-6D and concomitantly reduced plant height and kernel number,but had little effect on grain yield,contributing to lodging resistance without yield penalty.Hap I was subjected to positive selection in breeding.The findings provide a useful gene for wheat improvement and broaden insights into the regulatory machinery underpinning auxin-mediated quality formation,plant morphogenesis and yield gain.展开更多
基金supported by the National Natural Science Foundation of China(32372214 and 32272198)the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX(23)1035)+2 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD-2020-01)the Top Talent Supporting Program of Yangzhou University,China(YZU-2028-01)the Hong Kong Research Grants Council,China(GRF 12101722,12102423,and 12105824)。
摘要Highlights Improving spikelet production efficiency(SPE)can further enhance the grain yield and harvest index in rice.Higher SPE can enhance post-anthesis photoassimilate production in the shoots of rice.Higher SPE can promote the post-anthesis remobilization of non-structural carbohydrate(NSC)from rice stems.Rice grain yield is a complex agronomic trait determined by four key components:panicles per plant,spikelets per panicle,filled-grain rate,and grain weight.
基金financially supported by the Natural Science Foundation of Heilongjiang Province of China(No.YQ2021C010)the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA28070302).
摘要Intensive agricultural practices have stimulated progressive degradation of soil quality in the Mollisols of Northeast China,resulting in curtailed crop productivity.Although conservation tillage patterns have been modified to better suit Mollisols in recent years,whether conservation tillage can effectively enhance soil organic carbon(SOC)and crop yield remains controversial.In our study,the effects of two modified conservation tillage(MCT)patterns,the strip tillage with straw cover(STSC)and straw mixture amendment tillage(SMAT),on soil aggregate stability,SOC stock,enzyme activities,microbial communities,and crop yields were evaluated after five years of implementation.The results revealed that compared with conventional tillage(CT),both STSC and SMAT increased soil dissolved organic carbon(DOC),easily oxidized organic carbon(EOC),particulate organic carbon(POC),and mineral-bound organic carbon(MOC),thereby raising SOC content and reinforcing the stability of soil aggregates.The microbial taxa,Bacillus,Gemmatimonadaceae,Sphingomonas,Nocardioides,Blastococcus,Streptomyces,Trichoderma,Holtermanniella,Talaromyces,Didymella,and Fusicolla,were identified as potential contributors to carbon(C)sequestration.Soil microbial diversity and key taxa were positively correlated with SOC.Additionally,both STSC and SMAT raised the effective panicle number,kernels per ear,and 100-grain weight,as well as nitrogen and phosphorus absorption by maize grains and rhizomes,further increasing maize yield,with a more pronounced effect of SMAT than STSC.To sum up,the implementation of MCT can considerably strengthen C sequestration and crop productivity in the Mollisol region of Northeast China,contributing to farmland ecological protection and improved agroecological efficiency.
基金supported by the State Key Laboratory of Vegetable Biobreeding,China(Grant Nos.SKLVB2407,SKLVB2408,and SKLVB2504)the National Natural Science Foundation of China(Grant Nos.32300343,32570484,and 32572441)+2 种基金the Science and Technology Major Project of Anhui Province,China(Grant Nos.202423110050063,202423m10050002,and 2023n06020020)the Yangtze River Delta Science and Technology Innovation Community Joint Research(Basic Research)Project,China(Grant No.2024CSJZN01100)the Key Science and Technology Research Project of Xinjiang Production and Construction Corps,China(Grant No.2024AB013-2)。
摘要Precise manipulation of complex agronomic traits often requires the simultaneous introduction of mutations at multiple gene loci,a capability that remains challenging for existing genome-editing technologies.In this study,we developed and systematically compared five strategies for concurrent knockout and point mutation induction(KAPSi)based on prime editing,aiming to integrate precise nucleotide substitutions with efficient gene knockout within a single editing framework.
基金supported by the National Natural Science Foundation of China(Nos.42377009,42377006 and 41920104003)Guangdong Basic and Applied Basic Research Foundation(No.2024A1515011898)+1 种基金Guangzhou Basic Research Program(No.2024A04J4441)the Sino-French International Joint Laboratory ECOLAND established between Sun Yat-sen University,South China Agricultural University,Universitéde Lorraine and French National Institute for Agriculture,Food,and Environment(INRAE).
摘要Phytolacca americana is a known rare earth element(REE)hyperaccumulator,notable for its high biomass and rapid growth rate.However,its capacity to tolerate and accumulate REEs under high-exposure conditions is relatively limited,which restricts its utilization in agromining on soils heavily contaminated with REEs.Silicon(Si)has been shown to effectively mitigate metal(loid)s stress in various plants,and to promote REE tolerance in the well-known hyperaccumulator fern Dicranopteris linearis.This study aimed to assess the effects of Si on REE agromining using P.americana and to elucidate the underlying mechanisms.The results show that foliar Si application significantly increases the REEs accumulation of P.americana by as much as 73.6%and 172.4%in hydroponic and pot experiments respectively.Notably,Si application enhances the retention of REEs within the cell walls,primarily through the formation of silicate particles and/or modifications of the cell walls.Transcriptome analysis indicates that foliar Si application initially mitigates the toxicity of REEs by inducing an upregulation of genes associated with stress response and antioxidant enzymes,which facilitates the rapid elimination of accumulated reactive oxygen species.With increasing Si application,the abnormal overexpression of stress response genes,as well as genes involved in flavonoid biosynthesis and cell division,is alleviated,further implying that the entry of REE into the cytoplasm can be effectively hindered.This study shows that foliar Si application represents a potentially cost-efficiency agronomic practice for improvement of REE agromining using P.americana.
基金funded by the Chinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science Center(CATASCXTD202402)the Project of State Key Laboratory of Tropical Crop Breeding(SKLTCBYWF202503,NKLTCBCXTD24,and NKLTCBCXTD38)+1 种基金China Agriculture Research System of MOF and MARA(CARS-17)funding from Hainan University(XTC2022NYB04).
摘要Introduction:technical bottlenecks in genomic research on sugarcane The improvement of sugarcane(Saccharum spp.)is severely hindered by complex mixed ploidy,aneuploidy,and interspecific hybridization.Recently,Huang et al.constructed the first multiscale sugarcane pangenome graph integrating nine assemblies[1].Overcoming traditional linear reference limitations,this framework increased genomic diversity capture from 34%to 82%and identified key agronomic loci(e.g.,sugar content,leaf angle)via the novel dosage genome-wide association study(GWAS)method.We highlight this study's technical,statistical,and biological dimensions.
基金Qinghai Provincial Department of cience and Technology Key R&D and Transformation Project(2024-NK-106).
摘要This study aimed to elucidate the effects of varying seedling ages at planting on the agronomic traits and nutrient content of stem lettuce.The early-maturing variety“WS120”and the late-maturing variety“WS1”were employed as experimental materials.Four seedling age treatments were established at 20,25,30,and 35 d.By measuring the agronomic traits and nutrient content of the stem lettuce,we employed correlation analysis,principal component analysis,cluster analysis,and the membership function method for a comprehensive evaluation.This study aims to elucidate the optimal planting age for stem lettuce in plateau regions.By addressing the issues of inconsistent quality and yield that arise from arbitrary selection of planting age in production,we seek to establish a theoretical foundation for the development of a high-quality and efficient cultivation technology system for this crop.The results of the correlation analysis revealed that a total of 18 pairs of indicators in WS1 exhibited significant correlations,comprising 8 pairs that were positively correlated and 10 pairs that were negatively correlated.In WS120,25 pairs of indicators reached a significant correlation level,with 13 pairs positively correlated and 12 pairs negatively correlated.Principal component analysis identified 14 agronomic traits and 4 quality indicators,which were consolidated into 3 principal components,achieving cumulative contribution rates of 94.068%.Cluster analysis results indicated that WS1 could be categorised into two groups based on different treatments.At 30 d and 35 d,the samples were grouped together due to superior root-related indicators and nutritional components,whereas at 25 d and 20 d,they formed a separate group owing to enhanced agronomic traits.WS120 was classified into Group I,reflecting relatively favourable agronomic traits at 25 d and 20 d.Group II(30 d)was characterised by all indicators falling within the mid-range.Class III(35 d)was distinctly categorised due to elevated levels of vitamin C,soluble sugars,and soluble proteins.A comprehensive evaluation employing the membership function method indicated that the overall performance of WS1 and WS120 under varying treatments was ranked as follows:25 d>30 d>20 d>35 d.It can be utilised directly to inform production practices and holds considerable practical importance for advancing the high-quality and efficient cultivation of stem lettuce,thereby contributing to the growth of the plateau vegetable industry.
基金funded by the National Natural Science Foundation of China(Grant No.32070682)the International Science and Technology Cooperation Program of Hubei Province(Grant No.2023EHA048)the Start-up Fund of Wuhan University of Technology(Grant No.40121005).
摘要Jujube(Ziziphus jujuba Mill.)is a fruit crop of high economic value,renowned for its distinctive flavor and wide range of phenotypic diversity.Despite major advancements in jujube genomics,the role of genetic variants in underlying agronomic trait formation is still poorly understood.Here,we used seven high-quality jujube genomes to construct a pan-TE(transposon element)map and investigated how TEs shape genome evolution and agronomic traits.We found that TEs constitute 29.05%30.38%of the genome,predominantly long terminal repeat(LTR)retrotransposons such as Copia and Gypsy.A positive correlation(R2=0.76)between TE content and genome size underscores their role in genomic expansion.TE insertions within gene bodies significantly reduce gene expression,particularly for genes involved in cell wall biosynthesis and glucose metabolism.Population scale analysis of 1041 accessions identified 4176 transposable element insertion polymorphisms(TIPs)that distinguish wild and cultivated groups.Wild jujubes harbor stress-related TiPs(e.g.in peroxidase genes),whereas cultivated accessions carry TiPs linked to fruit development.Notably,a Gypsy insertion upstream of the cellulose synthase gene ZjCESA4 is associated with reduced expression and thinner pericarp in'Dongzao'compared to'Huizao'.Similarly,a downstream LTR/Gypsy insertion near the MADS-box transcription factor gene ZjAGL18 correlates with suppressed expression,highlighting the recurrent targeting of key regulatory genes by TEs during domestication.Our findings demonstrate that TiPs are a major source of genetic variation in jujube,providing molecular markers for breeding programs that aim to balance fruit quality and stress resilience.
基金supported by the China Agriculture Research Systemof MOF and MARA(CARS-28-01)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2021-RIP-01)the Agricultural Variety Improvement Project of Shandong Province(2022LZGC011).
摘要Pear(Pyrus L.)is a fruit tree of global commercial importance.Its genetic relationships,evolutionary history,dissemination routes,and genetic determinants ofmost agronomic traits remain to be elucidated.We conducted whole-genome resequencing of 495 Pyrus accessions.Phylogenetic and demographic analyses resolved geographic groupings of the accessions,identifying the Yunnan-Guizhou Plateau as the putative dissemination center for cultivated Pyrus pyrifolia and P.bretschneideri.Identification of two evolutionary bottlenecks provides insights into the population dynamics of pear species.Admixture and introgression analyses revealed both intraspecific and interspecific genetic exchanges,substantiating the complex emergence of cultivated populations.Genome-wide association study(GWAS)identified loci associated withnine crucial agronomic traits,together with eight candidate genes.The GWAS,molecular,and biochemical analyses suggested that PbeMADS25,PbeSPP,PbeDHQ-SDH,PbeARF2,PbePPO,PbePIN3,PbeCXE,and PbeMYB38 participate in the regulation of number of stigmas and number of locules,number of stamens,young leaf color,sepal persistence,astringency,acidity,aroma,and fruit skin color,respectively.Overexpression and metabonomic analysis of PbeCXE indicated that it affects the fruit aroma by affecting the balance between ester biosynthesis and substrate consumption.These findings expand our understanding of Pyrus evolution and provide a genomic foundation for genetic improvement of agronomic traits.
基金Supported by Science and Technology Project of China Tobacco Zhejiang Industrial Co.,Ltd.(2023330000340093).
摘要[Objectives]To investigate the effects of different planting densities and nitrogen application rates on the yield and quality of the tobacco cultivar Chuxue 80.[Methods]A field experiment was conducted in Hubei Province,evaluating various combinations of planting density and nitrogen rate for Chuxue 80.[Results]At the maturity stage,the TN1 treatment(5 kg N per 667 m2 with a density of 1900 plants per 667 m2)demonstrated the most favorable agronomic performance.The TN9 treatment(11 kg N per 667 m2 with a density of 1110 plants per 667 m2)achieved the highest wrapper tobacco yield and output value.Meanwhile,the TN5 treatment(8 kg N per 667 m2 with a density of 1515 plants per 667 m2)resulted in the best smoking quality.[Conclusions]The TN9 treatment,with a planting density of 1110 plants per 667 m2 and a nitrogen application rate of 11 kg per 667 m2,is recommended as the optimal cultivation practice for Chuxue 80 in Hubei Province.
摘要Medicinal plants are foundational to global health systems,yet their increasing integration into modern pharmaceutical supply chains has intensified sustainability challenges associated with climate change,biodiversity loss,and market volatility.Artemisia annua L.,the sole commercial source of artemisinin—the cornerstone of artemisinin-based combination therapies for malaria—exemplifies these tensions.Despite decades of agronomic and biotechnological research,global artemisinin supply remains unstable,largely due to yield variability,monoculture-driven ecological risks,and socio-economic vulnerabilities faced by smallholder producers.This literature-based review synthesizes advances across agronomy,climate-adaptive breeding,artificial intelligence–enabled precision agriculture,and socio-economic governance to propose an integrated framework for the sustainable expansion of A.annua cultivation.Central to the framework is the repositioning of agrobiodiversity—from a perceived constraint on productivity to a primary mechanism for enhancing ecological resilience,yield stability,and farmer livelihoods.The framework emphasizes biodiversity-centric agronomic practices,multi-latitude phenological adaptation,inclusive intelligent farming systems,and Public–Private–Farmer Partnerships(PPFPs)designed to promote equitable value distribution.While the framework is conceptual in nature,it is grounded in empirical evidence from diverse disciplinary domains.To facilitate translation into practice,a structured roadmap for empirical validation is proposed,outlining multi-location,multi-year field trials integrating agronomic,ecological,and socioeconomic metrics.Collectively,this work provides a systems-level blueprint for stabilizing the global artemisinin supply while aligning medicinal plant production with biodiversity conservation,climate resilience,and social equity objectives.
摘要To enhance soil fertility in Chinese orchards, this study conducts comprehensive research and applies specialized agricultural techniques. Addressing prevalent issues such as insufficient organic matter, rigid soil structure, nutrient imbalance, and declining biological activity, the paper thoroughly analyzes soil degradation patterns and their impacts on fruit yield and quality. The research framework comprises four sections: 1) Detailed implementation procedures for increasing soil organic content and improving physical properties through scientific approaches—including organic fertilizer application, green plant cultivation, surface grass coverage, and soil mulching; 2) Precise fertilization methods based on soil testing, micronutrient supplementation, and efficient irrigation systems combining drip and sprinkler irrigation; 3) Practical strategies utilizing functional microbial agents and treated carbon materials to optimize soil environment, promoting intercropping to boost microbial diversity, and maintaining ecosystem stability; 4) Integrated optimization of these techniques into a ready-to-implement agricultural solution validated through field trials, demonstrating its effectiveness in enhancing soil fertility, promoting healthy tree growth, and boosting economic returns. The objective is to establish a cost-effective, eco-friendly, and sustainable soil fertility improvement system tailored for long-term orchard applications, providing robust theoretical and technical support for sustained high-yield production.
基金supported by the Beijing Natural Science Foundation(6252007)the Beijing Nova Program,China(20220484023)+1 种基金the BAAFS Innovation Capacity Building Project(KJCX20240502)the BAAFS International Science and Technology Cooperation Platform(2024-08).
摘要To address the rising global agricultural labor costs,there is an urgent need for robots to accomplish some complex agronomic tasks and break through the limitations of traditional machinery.Thus,robots are considered an essential support for the future smart agriculture.Given that agronomic targets,such as plants and animals,are living organisms with diverse growth patterns and physical characteristics,effective hand–eye coordination is crucial for robots to interact with these targets proficiently.This paper reviews the developments in hand–eye coordination technology for agricultural robots,focusing on its configuration,principles,and applications in target detection and manipulation,based on a review of research literature and technical specifications of commercial products.Furthermore,the ongoing challenges in hand–eye coordination for robotic operations in complex agronomic tasks are analyzed and summarized,and the potential trends for overcoming these challenges are predicted.Finally,this review aims to deepen understanding of agricultural robots’capabilities and inspire ongoing innovation to further their agricultural applications.
基金supported by the National Key Research and Development Program of China(2021YFD1200600)the National Natural Science Foundation of China(32272105).
摘要Powdery mildew is a serious disease caused by Blumeria graminis f.sp.tritici(Bgt)that critically threatens the yield and quality of wheat(Triticum aestivum L.).Using effective powdery mildew resistance genes is the optimal method for controlling this disease.Against the background of high genetic homogeneity among the modern commercial cultivars that are mainly derived from conventional interbreeding,the resistance genes from wheat relatives have especially prominent advantages.Octoploid triticale,produced from common wheat and rye(Secale cereale L.)through distant hybridization,is a new synthetic species and valuable gene donor for wheat improvement.In this study,we developed the wheat-rye line YT5 through the hybridization of octaploid triticale and two wheat lines.YT5 was confirmed to be a 6RL ditelosomic addition and 1R(1B)substitution line using genomic in situ hybridization(GISH),multicolor fluorescence in situ hybridization(mc-FISH),multicolor GISH(mc-GISH)and molecular marker analysis.Genetic analysis showed that the powdery mildew resistance in YT5 was derived from the rye chromosome arm 6RL.After inoculation with different Bgt isolates at the seedling stage,YT5 had compound reaction patterns with both obvious spores and hypersensitivity,and it gradually became highly resistant until the adult-plant stage,thus showing a resistance response significantly different from the reported Pm genes from rye chromosome 6RL.YT5 also showed promising agronomic performance,so it is expected to be an elite resistance donor for wheat improvement.To promote the transfer of the chromosome arm 6RL of YT5 in marker-assisted selection(MAS)breeding,we selected and verified two 6RL-specific kompetitive allelespecific PCR(KASP)markers that can be applied to efficiently detect this chromosome arm in different wheat backgrounds.
基金supported by Research Fund for Young Talent Plans of Xi’an Jiaotong University(KZ6J007)the National Natural Science Foundation of China(62303372,GYKP034)
摘要THE development of agriculture faces significant challenges due to population growth,climate change,land depletion,and environmental pollution,threatening global food security[1].This necessitates the development of sustainable agriculture,where a fundamental step is crop breeding to improve agronomic or economic traits,e.g.,increasing yields of crops while decreasing resource usage and minimizing pollution to the environment[2].
基金supported by funding from Jiangxi Agricultural University(9232308314 to Huibin Han)Science and Technology Department of Jiangxi Province(20223BCJ25037 to Huibin Han and 20202ACB215002 to Shuaiying Peng)+1 种基金the Outstanding Youth Fund Project of the Natural Science Foundation of Jiangxi Province,China(20242BAB23066 to Yong Zhou)National Natural Science Foundation of China(32060047 to Jianping Liu,32160739 to Youxin Yang,32460797 to Yong Zhou and 32460081 to Huibin Han)。
摘要Small signaling peptides,generally comprising fewer than 100 amino acids,act as crucial signaling molecules in cell-to-cell communications.Upon perception by their membrane-localized corresponding receptors or co-receptors,these peptide-receptor modules then(de)activate either long-distance or local signaling pathways,thereby orchestrating developmental and adaptive responses via(post)transcriptional,(post)translational,and epigenetic regulations.The physiological functions of small signaling peptides are implicated in a multitude of developmental processes and adaptive responses,including but not limited to,shoot and root morphogenesis,organ abscission,nodulation,Casparian strip formation,pollen development,taproot growth,and various abiotic stress responses such as aluminum,cadmium,drought,cold,and salinity.Additionally,they play a critical role in response to pathogenic invasions.These small signaling peptides also modulate significant agronomic and horticultural traits,such as fruit size,maize kernel development,fiber elongation,and rice awn formation.Here,we underscore the roles of several small signaling peptide families such as CLE,RALF,EPFL,mi PEP,CEP,IDA/IDL,and PSK in regulating these biological processes.These novel insights will deepen our current understanding of small signaling peptides,and offer innovative strategies for genetic breeding stress-tolerant crops and horticultural plants,contributing to establish sustainable agricultural systems.
基金supported by funding from the National Key R&D Program of China(2023ZD0407304)the Sci-Tech Innovation 2030 Agenda(2022ZD0115703)Fundamental Research Funds for Central Non-Profit of Chinese Academy of Agricultural Sciences(Y2023PT20).
摘要Epigenetics-mediated breeding(epibreeding)involves engineering crop traits and stress responses through the targeted manipulation of key epigenetic features to enhance agricultural productivity.While conventional breeding methods raise concerns about reduced genetic diversity,epibreeding propels crop improvement through epigenetic variations that regulate gene expression,ultimately impacting crop yield.Epigenetic regulation in crops encompasses various modes,including histone modification,DNA modification,RNA modification,non-coding RNA,and chromatin remodeling.This review summarizes the epigenetic mechanisms underlying major agronomic traits in maize and identifies candidate epigenetic landmarks in the maize breeding process.We propose a valuable strategy for improving maize yield through epibreeding,combining CRISPR/Cas-based epigenome editing technology and Synthetic Epigenetics(SynEpi).Finally,we discuss the challenges and opportunities associated with maize trait improvement through epibreeding.
基金supported by the Centre de Coopération Internationale en Recherche Agronomique pour le Développement(CIRAD)and Genoscope(from French Alternative Energies and Atomic Energy Commission(CEA))the European Agricultural Fund for Rural Development(FEADER)and Région Guadeloupe through‘Plan Banane Durable 1’and‘Plan Banane Durable 2’programmes+1 种基金the France Génomique(ANR-10-INBS-09-08)project DYNAMOthe CGIAR Research Programme on Roots,Tubers and Bananas and the Agropolis Fondation(ID 1504-006)‘GenomeHarvest’project through the French Investissements d’Avenir programme(Labex Agro:ANR-10-LABX-0001-01)。
摘要Banana breeding is hampered by the very low fertility of domesticated bananas and the lack of knowledge about the genetic determinism of agronomic traits.We analysed a breeding population of 2723 triploid hybrids resulting from crosses between diploid and tetraploid Musa acuminata parents,which was evaluated over three successive crop cycles for 24 traits relating to yield components and plant,bunch,and fruit architectures.A subset of 1129 individuals was genotyped by sequencing,revealing 205612 single-nucleotide polymorphisms(SNPs).Most parents were heterozygous for one or several large reciprocal chromosomal translocations,which are known to impact recombination and chromosomal segregation.We applied two linear mixed models to detect associations between markers and traits:(i)a standard model with a kinship calculated using all SNPs and(ii)a model with chromosome-specific kinships that aims at recovering statistical power at alleles carried by long non-recombined haplotypic segments.For 23 of the 24 traits,we identified one to five significant quantitative trait loci(QTLs)for which the origin of favourable alleles could often be determined amongst the main ancestral contributors to banana cultivars.Several QTLs,located in the rearranged regions,were only detected using the second model.The resulting QTL landscape represents an important resource to support breeding programmes.The proposed strategy for recovering power at SNPs carried by long non-recombined rearranged haplotypic segments is an important methodological advance for future association studies in banana and other species affected by chromosomal rearrangements.
基金supported by the National Natural Sciences Foundation of China(32441004 to Y.Z.)National Key Research and Development Program of China(2022YFF1003201)+1 种基金STI 2030-Major Projects(2023ZD0407102)Youth Innovation Promotion Association of the Chinese Academy of Sciences(Y2021032).
摘要Alfalfa(Medicago sativa L.),a perennial legume forage,has been broadly cultivated owing to a variety of favorable characteristics,including comprehensive ecological adaptability,superior nutritive value and palatability,and nitrogen fixation capacity.The productivity traits of alfalfa,specifically its biomass yield and forage quality,are significantly influenced by a series of determinants,including internal developmental factors and external environmental cues.However,the regulatory mechanisms underlying the fundamental biological problems of alfalfa remain elusive.Here,we conducted a comprehensive review focusing on the genomics of alfalfa,advancements in gene-editing technologies,and the identification of genes that control pivotal agronomic characteristics,including biomass formation,nutritional quality,flowering time,and resistance to various stresses.Moreover,a molecular design roadmap for the‘ideal alfalfa’has been proposed and the potential of pangenomes,self-incompatibility mechanisms,de novo domestication,and intelligent breeding strategies to enhance alfalfa’s yield,quality,and resilience were further discussed.This review will provide comprehensive information on the basic biology of alfalfa and offer new insights for the cultivation of ideal alfalfa.
基金financially supported by Sichuan Science and Technology Program(2024YFHZ0302)Natural Science Foundation of Sichuan Province(2023NSFSC0158)+2 种基金Sichuan Fruit Innovation Team of National Modern Agricultural Industrial Technology System in China(SCCXTD-2024-4)the Project of Rural Revitalization Research Institute in Tianfu New Area of Sichuan Province(XZY1-04)Cherry Resources Sharing and Service Platform of Sichuan Province.
摘要Cherries are one of the economically important fruit crops in the Rosaceae family,Prunus genus.As the first fruits of the spring season in the northern hemisphere,their attractive appearance,intensely desirable tastes,high nutrients content,and consumer-friendly size captivate consumers worldwide.In the past 30 years,although cherry geneticists and breeders have greatly progressed in understanding the genetic and molecular basis underlying fruit quality,adaptation to climate change,and biotic and abiotic stress resistance,the utilization of cherry genomic data in genetics and molecular breeding has remained limited to date.Here,we thoroughly investigated recent discoveries in constructing genetic linkage maps,identifying quantitative trait loci(QTLs),genome-wide association studies(GWAs),and validating functional genes of edible cherries based on available de nouo genomes and genome resequencing data of edible cherries.We further comprehensively demonstrated the genetic architecture of the main agronomic traits of edible cherries by methodically integrating QTLs,GWAs loci,and functional genes into the identical reference genome with improved annotations.These collective endeavors will offer new perspectives on the availability of sequence data and the construction of an interspecific pangenome of edible cherries,ultimately guiding cherry breeding strategies and genetic improvement programs,and facilitating the exploration of similar traits and breeding innovations across Prunus species.
基金supported by the STI 2030-Major Projects(2023ZD0406903)the National Natural and Science Foundation of China(32272182)+1 种基金the Postdoctoral Fellowship Program of CPSF(GZC20241955)the Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS).
摘要High molecular weight glutenin subunits(HMW-GS),major components of seed storage proteins in wheat,have large effects on processing quality.GLU-1 genes encode HMW-GS and their expression is mainly controlled at the transcriptional level by interactions between cis-regulatory elements and transcription factors.We previously identified an Aux/IAA transcription factor TaIAA10-6D that bound to a conserved cis-regulatory module CCRM1-1,the most essential conserved cis-regulatory module in GLU-1.Here,we confirmed the binding of TaIAA10-6D to CCRM1-1 using yeast one hybrid and dualluciferase reporter assays.The enhanced expression of TaIAA10-6D suppressed glutenin accumulation and increased gliadin content.Dynamic transcriptome analyses revealed that TaIAA10-6D overexpression down-regulated glutenin and gliadin genes during an early stage of grain filling,but up-regulated gliadin genes during a late stage probably by endoplasmic reticulum stress,accounting for its effect on the tradeoff between glutenin and gliadin.Rheological property and processing quality assays showed that TaIAA10-6D overproduction reduced stabilization time and bread quality,but enhanced cookie quality.Overexpression of TaIAA10-6D also reduced plant height,leaf size,kernel number and grain yield.We identified two major haplotypes of TaIAA10-6D,Hap I and Hap II,and developed a breeding-friendly diagnostic marker.Hap I conferred higher expression of TaIAA10-6D and concomitantly reduced plant height and kernel number,but had little effect on grain yield,contributing to lodging resistance without yield penalty.Hap I was subjected to positive selection in breeding.The findings provide a useful gene for wheat improvement and broaden insights into the regulatory machinery underpinning auxin-mediated quality formation,plant morphogenesis and yield gain.