Chloroplast gene expression relies on nucleus-encoded factors for RNA metabolic processing,but the mechanisms under cold stress remain poorly understood.In this study,we isolated and characterized a foxtail millet(Set...Chloroplast gene expression relies on nucleus-encoded factors for RNA metabolic processing,but the mechanisms under cold stress remain poorly understood.In this study,we isolated and characterized a foxtail millet(Setaria italica)mutant,temperature-sensitive chlorophyll-deficient(sitcd1),which exhibited reduced chlorophyll content and abnormal chloroplasts,resulting in an albino phenotype during early leaf development at low temperatures(20℃during the day and 18℃at night,L20/D18).Map-based cloning revealed that SiTCD1 encodes a P-type PPR protein localized in chloroplasts.In sitcd1 background,transgenic lines overexpressing SiTCD1 had nearly normal green leaves under L20/D18 conditions.SiTCD1 was especially expressed in the earlier development of leaves at low temperatures.In addition,SiTCD1 bound directly to the plastid gene atpF in vitro,so it might participate in the splicing of plastid gene atpF at low temperatures.RNAseq results indicated that the expression of genes related to metabolism(such as porphyrin,chlorophyll and glutathione metabolism),which require ATP for energy,was down-regulated in sitcd1,resulting in reductions in chlorophyll content,reduced glutathione(GSH),and its redox couple(GSH/oxidized glutathione(GSSG))at low temperatures.As sitcd1 exhibited greater sensitivity at the bud bursting stage than germination or the seedling stage under cold stress,we identified two haplotypes of SiTCD1(SiTCD1Hap1 and SiTCD1Hap2)in 195 accessions,and found that accessions carrying the SiTCD1Hap2 allele were more tolerant to cold stress than those with the SiTCD1Hap1 allele at the bud bursting stage.In summary,our results suggest that Si TCD1 is essential for early chloroplast development at low temperatures in foxtail millet.展开更多
Whether talking about meeting industry or tourism, the coastal city Xiamen has unique position in China. As the first five-star hotel in XiaMen City even whole Fujian province, Xiamen Yeohwa Hotel with high level of s...Whether talking about meeting industry or tourism, the coastal city Xiamen has unique position in China. As the first five-star hotel in XiaMen City even whole Fujian province, Xiamen Yeohwa Hotel with high level of services and brilliant history, has become the first choice for a number of international politicians and business leaders. Singapore's former Minister Mentor Lee Kuan Yew signed an autography--Xiamen Yeohwa Hotel feels "like home". The hotel, which insisted to provide state-guest-level services to all of the customers, has certainly become a hallmark of Xiamen's tourism.展开更多
基金supported by the Central Guidance on Local Science and Technology Development Fund of Shanxi Province,China(YDZJSX2022A043)the Minor Crop Molecular Breeding Platform Special Project of Shanxi Academy of Agricultural Sciences,China(YGC2019FZ3)+3 种基金the Key R&D Projects of Shanxi Province,China(202102140601003)the Modern Agriculture Industry Technology System Construction Project of Shanxi Province,China(2024CYJSTX04-01)the Shanxi Province Agricultural Key Core Technology Research,China(NYGG19)the Shanxi Province Science and Technology Innovation Team Project,China(2015013001-09)。
摘要Chloroplast gene expression relies on nucleus-encoded factors for RNA metabolic processing,but the mechanisms under cold stress remain poorly understood.In this study,we isolated and characterized a foxtail millet(Setaria italica)mutant,temperature-sensitive chlorophyll-deficient(sitcd1),which exhibited reduced chlorophyll content and abnormal chloroplasts,resulting in an albino phenotype during early leaf development at low temperatures(20℃during the day and 18℃at night,L20/D18).Map-based cloning revealed that SiTCD1 encodes a P-type PPR protein localized in chloroplasts.In sitcd1 background,transgenic lines overexpressing SiTCD1 had nearly normal green leaves under L20/D18 conditions.SiTCD1 was especially expressed in the earlier development of leaves at low temperatures.In addition,SiTCD1 bound directly to the plastid gene atpF in vitro,so it might participate in the splicing of plastid gene atpF at low temperatures.RNAseq results indicated that the expression of genes related to metabolism(such as porphyrin,chlorophyll and glutathione metabolism),which require ATP for energy,was down-regulated in sitcd1,resulting in reductions in chlorophyll content,reduced glutathione(GSH),and its redox couple(GSH/oxidized glutathione(GSSG))at low temperatures.As sitcd1 exhibited greater sensitivity at the bud bursting stage than germination or the seedling stage under cold stress,we identified two haplotypes of SiTCD1(SiTCD1Hap1 and SiTCD1Hap2)in 195 accessions,and found that accessions carrying the SiTCD1Hap2 allele were more tolerant to cold stress than those with the SiTCD1Hap1 allele at the bud bursting stage.In summary,our results suggest that Si TCD1 is essential for early chloroplast development at low temperatures in foxtail millet.
摘要Whether talking about meeting industry or tourism, the coastal city Xiamen has unique position in China. As the first five-star hotel in XiaMen City even whole Fujian province, Xiamen Yeohwa Hotel with high level of services and brilliant history, has become the first choice for a number of international politicians and business leaders. Singapore's former Minister Mentor Lee Kuan Yew signed an autography--Xiamen Yeohwa Hotel feels "like home". The hotel, which insisted to provide state-guest-level services to all of the customers, has certainly become a hallmark of Xiamen's tourism.