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SiTCD1 encodes a P-type PPR protein that affects early chloroplast development at low temperatures in foxtail millet 认领 引用
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作者 Zhilan Wang Xiaofen Du +7 位作者 Kangni Han Miao Li Shichao Lian Yuxin Li Yanfang Li Linyi Zhang Xingchun Wang Jun Wang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第7期2739-2754,共16页
Chloroplast gene expression relies on nucleus-encoded factors for RNA metabolic processing,but the mechanisms under cold stress remain poorly understood.In this study,we isolated and characterized a foxtail millet(Set... Chloroplast gene expression relies on nucleus-encoded factors for RNA metabolic processing,but the mechanisms under cold stress remain poorly understood.In this study,we isolated and characterized a foxtail millet(Setaria italica)mutant,temperature-sensitive chlorophyll-deficient(sitcd1),which exhibited reduced chlorophyll content and abnormal chloroplasts,resulting in an albino phenotype during early leaf development at low temperatures(20℃during the day and 18℃at night,L20/D18).Map-based cloning revealed that SiTCD1 encodes a P-type PPR protein localized in chloroplasts.In sitcd1 background,transgenic lines overexpressing SiTCD1 had nearly normal green leaves under L20/D18 conditions.SiTCD1 was especially expressed in the earlier development of leaves at low temperatures.In addition,SiTCD1 bound directly to the plastid gene atpF in vitro,so it might participate in the splicing of plastid gene atpF at low temperatures.RNAseq results indicated that the expression of genes related to metabolism(such as porphyrin,chlorophyll and glutathione metabolism),which require ATP for energy,was down-regulated in sitcd1,resulting in reductions in chlorophyll content,reduced glutathione(GSH),and its redox couple(GSH/oxidized glutathione(GSSG))at low temperatures.As sitcd1 exhibited greater sensitivity at the bud bursting stage than germination or the seedling stage under cold stress,we identified two haplotypes of SiTCD1(SiTCD1Hap1 and SiTCD1Hap2)in 195 accessions,and found that accessions carrying the SiTCD1Hap2 allele were more tolerant to cold stress than those with the SiTCD1Hap1 allele at the bud bursting stage.In summary,our results suggest that Si TCD1 is essential for early chloroplast development at low temperatures in foxtail millet. 展开更多
关键词 foxtail millet(Setaria italica) chloroplast development SiTCD1 plastid gene atpF low temperature
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半夏及近缘种叶绿体非编码区序列分析 认领 引用 被引量:5
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作者 郑丹书 张君毅 郭巧生 《中草药》 CAS CSCD 北大核心 2013年第7期881-886,共6页
目的通过测定半夏及近缘种的DNA叶绿体的非编码区序列,为半夏的分子鉴别和遗传多样性研究提供依据。方法在我国半夏主要分布区收集到43份半夏及滴水珠和虎掌半夏2个近缘种,采用PCR法克隆叶片基因组DNA中psbK-psbI和atpF-atpH两段序列,... 目的通过测定半夏及近缘种的DNA叶绿体的非编码区序列,为半夏的分子鉴别和遗传多样性研究提供依据。方法在我国半夏主要分布区收集到43份半夏及滴水珠和虎掌半夏2个近缘种,采用PCR法克隆叶片基因组DNA中psbK-psbI和atpF-atpH两段序列,借助生物信息学软件进行比对分析。结果半夏atpF-atpH序列长为337~342bp,较为保守,仅含变异位点7个,其中信息位点1个,遗传距离为0~0.024。半夏psbK-psbI序列长为432~435bp,变异位点37个,其中信息位点17个,遗传距离为0~0.069。聚类分析结果均显示出与表型以及地理居群的不一致。结论 psbK-psbI序列较atpF-atpH有更好的鉴别能力,在半夏种内具有较丰富的变异位点。 展开更多
关键词 半夏 psbK-psbI atpF-atpH 分子鉴定 非编码区序列 遗传多样性
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利用分子遗传标记分析浙江地区浮萍多样性 认领 引用 被引量:8
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作者 王敏雅 陆菲 +5 位作者 毛杉杉 李岚 张琼 刘莉 采克俊 曹访 《安徽农业科学》 CAS 2012年第31期15127-15128,15218,共2页
[目的]利用分子遗传标记对浙江地区的浮萍多样性进行分析。[方法]收集浙江省不同地区的6株浮萍,通过形态学特征、叶绿体基因组的rpS16内含子序列及atpF-atpH条形码序列分析浮萍的遗传多样性。[结果]形态学鉴定表明,HN-YG-3和HZ-YXY-1浮... [目的]利用分子遗传标记对浙江地区的浮萍多样性进行分析。[方法]收集浙江省不同地区的6株浮萍,通过形态学特征、叶绿体基因组的rpS16内含子序列及atpF-atpH条形码序列分析浮萍的遗传多样性。[结果]形态学鉴定表明,HN-YG-3和HZ-YXY-1浮萍属紫萍属,而HZ-HZY-2、HA-LP-4、HA-HSD-5和ZS-DH-6浮萍属浮萍属。atpF-atpH序列分析表明,HZ-HSD-5浮萍和HZ-HZY-2浮萍亲缘关系最近;rpS16内含子序列分析表明,ZS-DH-6浮萍和HA-HSD-5浮萍亲缘关系最近。[结论]叶绿体基因组和atpF-atpH条形码序列分子遗传标记较形态学鉴定更能反映出浮萍遗传多样性的特点,为今后浮萍资源的开发利用提供了更多的参考依据。 展开更多
关键词 浮萍 rpS16 atpF-atpH 分子标记 遗传多样性
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长江中游浮萍种质资源鉴定及遗传多样性分析 认领 引用 被引量:1
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作者 穆瑶佳 谭德冠 +4 位作者 于博涵 付莉莉 陈婷 孙雪飘 张家明 《植物遗传资源学报》 CAS CSCD 北大核心 2020年第4期938-944,共7页
浮萍为浮萍科水生植物,具有生长繁殖快、氮磷吸收能力强及高含蛋白质和淀粉的特性,在饲料和能源方面具有较大的开发潜力。目前尚未有系统研究我国长江中游地区浮萍种质资源的报道。本研究从我国长江中游地区3个省42个具有代表性的自然... 浮萍为浮萍科水生植物,具有生长繁殖快、氮磷吸收能力强及高含蛋白质和淀粉的特性,在饲料和能源方面具有较大的开发潜力。目前尚未有系统研究我国长江中游地区浮萍种质资源的报道。本研究从我国长江中游地区3个省42个具有代表性的自然水体中共收集到浮萍种质112份。经形态学并结合分子生物学鉴定,它们分属4个属6个种,其中青萍属(Lemna L.)含3个种54份种质;多根紫萍属(Spirodela Schleid.)、少根紫萍属(Landoltia Les&Crawford)、芜萍属(Wolffia Horkel ex Schleid.)均只有1个种,种质数量分别为33份、19份和6份。遗传多样性分析表明Atp F-Atp H间隔序列和rp S16内含子序列的单倍型数量分别为11和18,单倍型多样性分别为0.748和0.892,群体突变率分别为0.03013和0.03507,平均每Kb核苷酸差异数为31.386和61.263,核苷酸多样性分别为0.04713和0.06623。本研究为我国长江中游浮萍的资源化利用奠定了基础。 展开更多
关键词 浮萍 种质资源 遗传多样性 AtpF-AtpH rpS16
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To require attendant with State-guestlevel service 认领 引用
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《中国会展》 2012年第2期94-95,共2页
Whether talking about meeting industry or tourism, the coastal city Xiamen has unique position in China. As the first five-star hotel in XiaMen City even whole Fujian province, Xiamen Yeohwa Hotel with high level of s... Whether talking about meeting industry or tourism, the coastal city Xiamen has unique position in China. As the first five-star hotel in XiaMen City even whole Fujian province, Xiamen Yeohwa Hotel with high level of services and brilliant history, has become the first choice for a number of international politicians and business leaders. Singapore's former Minister Mentor Lee Kuan Yew signed an autography--Xiamen Yeohwa Hotel feels "like home". The hotel, which insisted to provide state-guest-level services to all of the customers, has certainly become a hallmark of Xiamen's tourism. 展开更多
关键词 商品展览 CCC 市场 ATPF
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