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Bacterial biosurfactant-reinforced chitooligosaccharide/polyvinyl alcohol hydrogels accelerate methicillin-resistant Staphylococcus aureus-infected wound healing by attenuating its virulence factors 认领 引用
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作者 Geum-Jae Jeong Dong-Joo Park +6 位作者 Ju-Hong Kang Se-Chang Kim Yu-Jin Ahn Kyung-Jin Cho Fazlurrahman Khan Won-Kyo Jung Young-Mog Kim 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS CSCD 2026年第1期125-149,共25页
Methicillin-resistant Staphylococcus aureus(MRSA) causes widespread infections and poses serious public health concerns. Its high level of resistance to multiple antibiotics has garnered growing interest in identifyin... Methicillin-resistant Staphylococcus aureus(MRSA) causes widespread infections and poses serious public health concerns. Its high level of resistance to multiple antibiotics has garnered growing interest in identifying and applying novel antibacterial compounds derived from natural sources. In this study, we purified a biosurfactant(BS) from Bacillus rugosus HH2 to develop a natural antibacterial agent. This agent was then reinforced with chitooligosaccharide(COS) and polyvinyl alcohol(PVA) to create a hydrogel that promoted healing in MRSA-infected wounds. The COS/PVA/BS hydrogel was readily fabricated via the freeze-thaw method and demonstrated excellent mechanical strength, biological activity,and biocompatibility. In vitro assays confirmed that the hydrogel significantly enhanced the proliferation, migration, angiogenesis, and extracellular matrix deposition of fibroblasts,keratinocytes, and endothelial cells. Moreover, it exhibited strong bacteriostatic and bactericidal activities against MRSA, along with potent antibiofilm activity and inhibition of virulence factors relevant to MRSA-induced wound infections. Its anti-virulence effects have been linked to the downregulation of quorum sensing and virulence-related genes in MRSA. In an in vivo model of MRSA-induced infection, the COS/PVA/BS hydrogel significantly accelerated wound healing and markedly reduced the MRSA burden. Immunofluorescence staining confirmed enhanced neovascularization and regulated macrophage responses,underscoring the angiogenic and immunomodulatory effects of the hydrogel. Overall,the COS/PVA/BS hydrogel represents a promising therapeutic strategy for addressing antibiotic-resistant bacterial infections and promoting wound repair, supported by the use of common raw materials, a simple fabrication process, and high-yield production of natural antibacterial agents. 展开更多
关键词 Hydrogel Biosurfactant Methicillin-resistant Staphylococcus aureus Bacterial infection Wound healing Virulence factor
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Boosting Fosfomycin Efficacy Against Methicillin-Resistant Staphylococcus aureus Infections by Targeting Pyrimidine Metabolism 认领 引用
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作者 Jianya Luo Qingyan Lv +2 位作者 Mengping He Zhiqiang Wang Yuan Liu 《Engineering》 SCIE EI CSCD 2026年第5期127-141,共15页
Methicillin-resistant Staphylococcus aureus(MRSA)represents a significant global public health threat.Combination therapy,particularly the use of antibiotics in conjunction with non-antibiotic agents,has emerged as a ... Methicillin-resistant Staphylococcus aureus(MRSA)represents a significant global public health threat.Combination therapy,particularly the use of antibiotics in conjunction with non-antibiotic agents,has emerged as a promising strategy to address the growing crisis of antibiotic resistance.Fosfomycin(FOS),increasingly utilized in clinical practice for treating drug-resistant bacterial infections,exhibits limited efficacy as a monotherapy.Here,we find that 5-fluorouracil(5-FU),an US Food and Drug Administration(FDA)-approved anticancer drug,effectively enhances the antibacterial activity of FOS against MRSA,including biofilm-embedded MRSA cells.Mechanistically,5-FU targets cytidine triphosphate(CTP)synthase,a rate-limiting enzyme responsible for the adenosine triphosphate(ATP)-dependent conversion of uridine triphosphate(UTP)to CTP.Moreover,we demonstrate that the synergistic effect of 5-FU and FOS arises from the perturbation of pyrimidine metabolism,which induces membrane damage,dissipation of the proton motive force(PMF),enhanced ATP synthesis,and accumulation of reactive oxygen species,culminating in bacterial death.In both Galleria mellonella(G.mellonella)and murine infection models,the combination of 5-FU and FOS markedly improves survival and reduces bacterial burdens.Collectively,our work demonstrates the therapeutic potential of 5-FU combined with FOS for tackling MRSA infections and highlights the pivotal role of perturbing pyrimidine metabolism in restoring antibiotic susceptibility. 展开更多
关键词 Antibiotic adjuvant 5-Fluorouracil Fosfomycin MRSA Pyrimidine metabolism
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Synergistic antibacterial and anti-inflammatory potentials of dual-loaded self-healing hydrogel for methicillin-resistant Staphylococcus aureus-infected wound healing 认领 引用
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作者 Sangyu Hu Weigang Zhong +5 位作者 Yuzhu Pei Yutong Zhou Jianfeng Wang Xuming Deng Zihao Teng Lei Xu 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2026年第1期242-256,共15页
The emergence of drug-resistant bacterial infection and persistent biofilm colonization pose a rigorous challenge to effective wound healing and regeneration,necessitating the innovative therapeutic strategies to comb... The emergence of drug-resistant bacterial infection and persistent biofilm colonization pose a rigorous challenge to effective wound healing and regeneration,necessitating the innovative therapeutic strategies to combat these pressing clinical crises.Herein,nortriptyline,a novel United States Food and Drug Administration(FDA)-approved tricyclic antidepressant was uncovered to effectively potentiate bactericidal activities ofβ-lactam antibiotics against methicillin-resistant Staphylococcus aureus(MRSA).Mechanistically,nortriptyline functions by disrupting the microbial iron homeostasis and potentiation of Fenton chemistry-mediated oxidative stress,concomitant with metabolic reprogramming via tricarboxylic acid(TCA)cycle dysregulation and membrane destabilization.To enhance combination therapy-mediated therapeutic potential in wound management,the dual-loaded self-healing hydrogel OHA-PLL@AN was engineered to exhibit excellent biocompatibility and antibacterial potentials through molecular cross-linking of oxidized hyaluronic acid(OHA)andε-polylysine(PPL).The therapeutic efficacy of OHA-PLL@AN was further validated in a murine model with MRSA-infected cutaneous wounds.OHA-PLL@AN therapy significantly attenuated the inflammatory response,concurrently promoting angiogenesis and accelerating the cutaneous wounds healing.Collectively,these findings underscore the dual drug-loaded self-healing hydrogel OHA-PLL@AN with anti-infection and anti-inflammatory properties as a novel therapeutic strategy for drug-resistant bacterial infected wounds therapy. 展开更多
关键词 MRSA Antibacterial Nortriptyline β-lactam antibiotics Hydrogel Wound healing
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Farrerol as an innovative antivirulence agent disrupting virulence factors to combat foodborne pathogenicity in Staphylococcus aureus 认领 引用
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作者 Hangqian Yu Yuxin Zhang +2 位作者 Yuxin Yang Xiaoping Zhou Dacheng Wang 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第5期2474-2487,共14页
The overuse of antibiotics has accelerated the emergence of resistant bacteria,posing critical threats to food safety and public health.This challenge underscores the urgent need for alternative strategies,such as ant... The overuse of antibiotics has accelerated the emergence of resistant bacteria,posing critical threats to food safety and public health.This challenge underscores the urgent need for alternative strategies,such as antivirulence therapy,which disarms pathogens by targeting virulence factors rather than inhibiting bacterial growth.In this study,we identified farrerol,a flavonoid,as a potent inhibitor of the caseinolytic peptidase P(ClpP)protease,a key virulence regulator in Staphylococcus aureus,with an IC50of 19.33μg/mL.Farrerol significantly disrupts virulence factor secretion and reduces pathogenicity,demonstrating its potential to control S.aureus-induced foodborne illnesses.Mechanistic studies using fluorescence resonance energy transfer(FRET),thermal shift assay(TSA),cellular thermal shift assay(CETSA),fluorescence quenching,and molecular docking revealed that farrerol interacts with ClpP via key residues(GLY-127,GLN-132,and ASP-170).In vivo,farrerol reduced the bacterial load and improved survival in a Galleria mellonella infection model.Our findings demonstrated that farrerol is an effective antivirulence agent,offering an innovative approach for the food industry to control S.aureus-induced foodborne infections.This approach holds great potential for enhancing food safety and mitigating antibiotic resistance. 展开更多
关键词 Foodborne Staphylococcus aureus Farrerol Drug resistance Caseinolytic peptidase P
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Staphylococcus aureus Cultured from Sushi in China:Revealing the Potential Foodborne Pathogenic Risks based on Antimicrobial Susceptibility,Virulence Factors and Genetic Characteristics 认领 引用
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作者 Hui Li Xin Gan +6 位作者 Menghang Li Shiwei Liu Dajin Yang Lin Yan Séamus Fanning Li Bai Zhaoping Liu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2026年第2期239-244,共6页
Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contribu... Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contributors to staphylococcal food poisoning(SFP),causing vomiting,diarrhea,abdominal pain,headache,muscle cramps,and other acute gastroenteritis symptoms.More than 25 SEs and staphylococcal enterotoxin-like toxins(SE/s)have been described and which together comprise a superfamily of pyrogenic toxin superantigens(SAgs)[2]. 展开更多
关键词 bacterial foodborne illnesses staphylococcus aureus saureus foodborne pathogenic risks staphylococcal food poisoning sfp causing pyrogenic toxin sup antimicrobial susceptibility genetic characteristics virulence factors
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活性氧响应纳米粒嵌合油凝胶通过铁代谢−胶原重构协同促MRSA创面无瘢痕愈合 认领 引用
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作者 任玮晔 程苇钇 +4 位作者 陈井泉 何李婷 朴寄纲 魏颖慧 包丹丹 《药学学报》 CAS CSCD 北大核心 2026年第2期624-635,共12页
耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)感染严重阻碍皮肤创面愈合,常伴随炎症持续与瘢痕增生,临床治疗亟需更有效的局部治疗策略。本研究构建了一种基于天然活性成分的活性氧(reactive oxygen sp... 耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)感染严重阻碍皮肤创面愈合,常伴随炎症持续与瘢痕增生,临床治疗亟需更有效的局部治疗策略。本研究构建了一种基于天然活性成分的活性氧(reactive oxygen species,ROS)响应纳米粒嵌合油凝胶系统,集抗菌、抗炎及抗瘢痕多重功能于一体,用于MRSA感染创面的综合治疗。该油凝胶系扁柏酚与维替泊芬(verteporfin,VP)自组装纳米粒嵌合于β-谷甾醇构建的油凝胶中,其可贴合不规则创面,并在感染部位ROS刺激下响应释放药物。扁柏酚在局部与Fe3+螯合,破坏细菌铁代谢,诱导膜损伤并破坏DNA,实现非抗生素杀菌;VP通过抑制Yes相关蛋白/具有PDZ结合基序的转录共激活因子(Yes-associated proteinranscriptional co-activator with PDZ-binding motif,YAP/TAZ)通路,减少成纤维细胞过度活化,促进组织再生并抑制瘢痕形成;胶凝剂β-谷甾醇具备一定抗炎活性并辅助调控早期免疫反应。该制剂可发挥抗菌、抗炎及瘢痕修复等多重协同作用,为感染性慢性创面治疗提供新策略。动物实验经浙江中医药大学动物实验研究中心批准,且实验均按相关指导原则和规定进行,批准号:IACUC-20241125-07。 展开更多
关键词 活性氧响应 纳米粒 油凝胶 耐甲氧西林金黄色葡萄球菌 创面修复 递送系统
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赤芍抗MRSA活性及初步作用机制研究 认领 引用
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作者 高翔 马红叶 +3 位作者 胡守奎 宋瑾 张会存 郭宏林 《国际检验医学杂志》 CAS 2026年第13期1545-1548,1557,共4页
目的探究赤芍对耐甲氧西林金黄色葡萄球菌(MRSA)细菌毒力因子[α-溶血素(Hlα)、细胞间黏附基因(ica)操纵子中的icaA]的调控机制。方法采用微量肉汤稀释法测定赤芍水煎液对MRSA标准株ATCC43300的最低抑菌浓度(MIC),通过溶血试验观察赤芍... 目的探究赤芍对耐甲氧西林金黄色葡萄球菌(MRSA)细菌毒力因子[α-溶血素(Hlα)、细胞间黏附基因(ica)操纵子中的icaA]的调控机制。方法采用微量肉汤稀释法测定赤芍水煎液对MRSA标准株ATCC43300的最低抑菌浓度(MIC),通过溶血试验观察赤芍对Hlα溶血活性的影响,采用结晶紫染色法评估赤芍对生物被膜形成的影响,并利用实时荧光定量PCR(qPCR)检测赤芍对Hlα与生物被膜关键基因icaA转录水平的影响。结果赤芍水煎液对MRSA(ATCC43300)的MIC值为31.25mg/mL。溶血试验及qPCR结果显示,与对照组比较,赤芍能显著抑制Hlα的溶血活性及其基因的表达,且呈浓度依赖性。结晶紫染色表明赤芍可有效抑制生物被膜的形成。qPCR检测结果显示,与对照组比较,赤芍能显著下调icaA基因的转录,抑制多细胞间黏附素的合成,最终有效抑制生物被膜的形成。结论赤芍水煎液不仅具有直接的体外抑菌活性,更能在亚抑菌浓度下通过抑制Hlα的表达及生物被膜形成相关基因icaA的转录表达,有效削弱MRSA的关键毒力并阻断生物被膜的形成。 展开更多
关键词 赤芍 耐甲氧西林金黄色葡萄球菌 α-溶血素 生物被膜 icaA基因
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乳腺派杰氏病患者术后手术部位感染MRSA分析 认领 引用
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作者 兰圣美 施桂芳 +5 位作者 赵丽丽 宋艳萍 张玲 闫玲 黄化云 王毅 《医学检验与临床》 2026年第3期34-37,共4页
目的:调查乳腺派杰氏病患者手术部位感染耐甲氧西林金黄色葡萄球菌(Methicillin-resistant staphylococcus aureus,MRSA)的原因并提出改进措施,旨在为此类患者手术部位感染防控提供理论依据。方法:某综合医院手术部位感染目标性监测到1... 目的:调查乳腺派杰氏病患者手术部位感染耐甲氧西林金黄色葡萄球菌(Methicillin-resistant staphylococcus aureus,MRSA)的原因并提出改进措施,旨在为此类患者手术部位感染防控提供理论依据。方法:某综合医院手术部位感染目标性监测到1例乳腺派杰氏病患者发生MRSA手术部位感染。通过回顾性分析,系统查找患者术前、术中及术后可能引起手术部位感染的相关因素。采集病房和手术室空气、仪器设备表面、工作人员手、患者衣物、生活用品等标本送微生物实验室进行培养。结果:多份标本的菌落计数均超过标准,如皮肤、衣服、患者的陪伴椅、床栏等。微生物培养均培养出耐甲氧西林金黄色葡萄球菌。结论:术前正确准备手术部位皮肤,彻底清除手术切口部位和周围皮肤的污染,对皮肤湿疹样病变做好目标性抹除定植,可预防乳腺派杰氏病患者发生手术部位感染。 展开更多
关键词 乳腺派杰氏病 手术部位感染 耐甲氧西林金黄色葡萄球菌 皮肤消毒 去定植
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利奈唑胺联用血必净通过抑制Hla-NLRP3通路减轻MRSA肺炎小鼠的炎症损伤 认领 引用
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作者 尹梦霞 李蕊臣 +2 位作者 杨凯博 焦伟杰 刘新光 《南方医科大学学报》 CAS CSCD 北大核心 2026年第2期412-422,共11页
目的探讨利奈唑胺(LZD)联用血必净对耐甲氧西林金黄色葡萄球菌(MRSA)感染引起的小鼠肺炎的协同保护作用及作用机制。方法通过气管滴注MRSA菌液构建肺炎小鼠模型。将60只SPF级雄性C57BL/6J小鼠随机分为6组(n=10):对照组、模型组、血必净... 目的探讨利奈唑胺(LZD)联用血必净对耐甲氧西林金黄色葡萄球菌(MRSA)感染引起的小鼠肺炎的协同保护作用及作用机制。方法通过气管滴注MRSA菌液构建肺炎小鼠模型。将60只SPF级雄性C57BL/6J小鼠随机分为6组(n=10):对照组、模型组、血必净单药组(13 mL·kg-1·d-1)、LZD单药组(80 mg·kg-1·d-1)、血必净+LZD低剂量组(血必净6.5 mL·kg-1·d-1+LZD 80 mg·kg-1·d-1)及血必净+LZD高剂量组(血必净13 mL·kg-1·d-1+LZD 80 mg·kg-1·d-1)。通过体外抑菌实验和溶血活性实验检测血必净和LZD对MRSA的最小抑菌浓度(MIC)及对MRSA生长曲线及溶血活性的影响。通过HE染色观察肺组织病理学改变,ELISA法检测肺匀浆中炎症因子IL-6、TNF-α、IL-1β和IL-18含量。qPCR实验检测MRSA Hla、NLRP3、Caspase-1和ASC的mRNA水平。Western blotting实验分析MRSA Hla、NLRP3、Caspase-1和ASC蛋白表达。免疫组织化学法检测各组小鼠肺组织NLRP3、Caspase-1和ASC蛋白表达量。结果LZD联用血必净可提高MRSA肺炎小鼠的生存率,降低MRSA肺炎小鼠的肺组织炎症细胞浸润及肺损伤评分(P<0.01);LZD联用血必净降低MRSA肺炎小鼠肺匀浆的IL-6、TNF-α、IL-1β和IL-18的含量(P<0.05),并抑制小鼠肺组织MRSA Hla、NLRP3、Caspase-1和ASC的mRNA水平和蛋白的表达(P<0.05)。血必净对MRSA无抑菌活性,且对MRSA的增殖无抑制作用,但可在体内外抑制MRSA分泌α-溶血素(Hla),进而通过NLRP3相关通路作用抑制炎症反应,而LZD无此作用。结论LZD与血必净联用可增强其改善MRSA肺炎的药效,其机制可能为血必净可通过抑制α-溶血素分泌进而降低NLRP3介导的炎症反应。 展开更多
关键词 血必净注射液 利奈唑胺 MRSA 肺炎 NLRP3炎症小体 α-溶血素
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Prevalence,virulence genes,antimicrobial susceptibility,and genetic diversity of Staphylococcus aureus from young people from 18 to 25-year-old age in Ho Chi Minh City,Vietnam 认领 引用
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作者 Hong-Phuc Nguyen Truc-Anh Nguyen-Thi +2 位作者 Tuan-Loc Le Trang Nguyen Thi Xuan Hieu Vu-Quang 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2026年第4期181-188,共8页
Objective:To investigate the prevalence,antimicrobial resistance and genetic diversity of Staphylococcus(S.)aureus among healthy young adults of 18 to 25 years in Ho Chi Minh City,Vietnam.Methods:A cross-sectional,des... Objective:To investigate the prevalence,antimicrobial resistance and genetic diversity of Staphylococcus(S.)aureus among healthy young adults of 18 to 25 years in Ho Chi Minh City,Vietnam.Methods:A cross-sectional,descriptive study was conducted between December 2023 and December 2024.Nasal swabs were processed for S.aureus isolation,antibiotic susceptibility testing,and PCR-based detection of spa,pvl,and mecA genes.Genetic similarity was assessed using the unweighted pair group method with arithmetic mean cluster analysis.Statistical significance was determined by Chi-square test.Results:In this cross-sectional study of 428 students,the S.aureus carriage rate was 33.9%.Methicillin-resistant S.aureus(MRSA)prevalence was 54.5%(79/145).10%-17%of MRSA isolates exhibited resistance to three to ten antibiotics,with two strains demonstrating resistance to 11 and 12 drugs,respectively.The pvl gene was detected in 5.8%of isolates.55.17%S.aureus strains belonged to ten distinct spa types with 300-bp amplicons.Conclusions:These findings provide valuable insights into S.aureus carriage patterns among youth in Ho Chi Minh City,informing public health strategies for prevention and treatment. 展开更多
关键词 Staphylococcus aureus Antibiotic resistance mecA spa pvl
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Discovery of A New Sesquiterpene-Lactone from Streptomyces aureus SP-371 认领 引用
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作者 Zhang Tao Ji Zhenyu +1 位作者 Tang Gongli Hou Xianfeng 《有机化学》 SCIE CAS CSCD 北大核心 2026年第2期689-692,共4页
new sesquiterpene-lactone,designated as neopentalenolactone D1(1),was isolated from the fermentation cultures of Streptomyces aureus SP-371,which is known for producing agricultural-bactericide aureonuclemycin.The che... new sesquiterpene-lactone,designated as neopentalenolactone D1(1),was isolated from the fermentation cultures of Streptomyces aureus SP-371,which is known for producing agricultural-bactericide aureonuclemycin.The chemical structure of compound 1 was determined to be a neopentapyrrole natural product with a 5/5/6 tricyclic skeleton and one amide functional group,by using spectroscopic techniques such as nuclear magnetic resonance(1D/2D NMR)and high-resolution mass spectrometry(HR-ESI-MS).Its structural information indicates that it is probably the shunt product of neopentalenolactone biosynthetic pathway.In antibacterial activity evaluations,compound 1 demonstrated no inhibitory effects against the tested strains including Escherichia coli(DH5a),Staphylococcus aureus,and Bacillus subtilis under the experimental conditions. 展开更多
关键词 Streptomyces aureus sesquiterpene-lactone structural elucidation biosynthetic pathway
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基于TMT定量蛋白质组学揭示单宁酸对MRSA的抗菌机制 认领 引用 被引量：1
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作者 伍忠旭 彭玮 +4 位作者 杨海 刘鑫 杨苗 王爽 周永辉 《中国抗生素杂志》 CAS CSCD 北大核心 2026年第4期431-440,共10页
目的本研究采用串联质量标签(tandem mass tag,TMT)定量蛋白质组学探讨单宁酸对耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)的抑菌机制。方法药敏试验首先采用肉汤微量稀释法测定单宁酸对MRSA的最小... 目的本研究采用串联质量标签(tandem mass tag,TMT)定量蛋白质组学探讨单宁酸对耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)的抑菌机制。方法药敏试验首先采用肉汤微量稀释法测定单宁酸对MRSA的最小抑菌浓度(minimum inhibitory concentration,MIC)和最小杀菌浓度(minimal bactericidal concentration,MBC);采用TMT定量蛋白组学技术研究单宁酸作用后MRSA差异表达的蛋白,随后应用qRT-PCR方法对随机选取的差异表达蛋白进行验证。再利用软件对差异表达蛋白进行GO和KEGG pathway富集分析及蛋白相互作用(PPI)分析,筛选富集分析及蛋白相互作用分析排名靠前的核心蛋白进行分子对接,以验证单宁酸与MRSA中关键蛋白的相互作用。结果单宁酸的MIC为0.625 mg/mL,MBC为1.25 mg/mL。在1/2 MIC单宁酸干预作用下,共鉴定到差异表达蛋白208个,其中上调蛋白127个,下调蛋白81个。GO分析显示,差异表达蛋白亚细胞结构定位主要是:细胞质占55.29%、未知细胞成分占21.63%,细胞膜占9.13%、细胞外基质占8.65%、细胞壁占5.29;qRT-PCR结果显示,随机筛选的差异蛋白对应的基因(glnA、ribD、clpB、gap和lukE)转录水平与组学数据一致(P<0.05);KEGG pathway富集分析发现,五倍子单宁酸干预后,单宁酸能够显著影响MRSA的氮代谢和碳代谢途径,包括嘧啶代谢、嘌呤代谢、组氨酸代谢、精氨酸生物合成、甲烷代谢、柠檬酸循环等;PPI分析显示相互作用蛋白主要集中在核苷酸生物合成和DNA复制修复,随后选取排名靠前的蛋白与单宁酸进行分子对接分析,结果显示单宁酸与核心靶点蛋白InfB、RplB、RplK、RpIO具有较强的结合,其中与RplB的结合最为紧密。结论本研究通过探讨单宁酸对MRSA的抑菌机制,发现单宁酸与参与蛋白质生物合成、核苷酸生物合成和DNA复制修复相关的蛋白能够产生相互作用,尤其是单宁酸与RplB蛋白的相互作用,从而抑制MRSA的生长,为深入研究单宁酸抑制MRSA机制提供依据。 展开更多
关键词 单宁酸 耐甲氧西林金黄色葡萄球菌 TMT蛋白质组学 抗菌机制
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Unveiling the antibiotic resistance pattern in Staphylococcus aureus:A systematic review(2013-2024) 认领 引用
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作者 Snehal M Deshmukhe Chanda R Vyawahare +2 位作者 Poonam V Suryawanshi Manisha M Ratnaparkhi Nageswari R Gandham 《World Journal of Experimental Medicine》 2026年第2期86-98,共13页
BACKGROUND Staphylococcus aureus(S.aureus)is one of the six highly pathogenic nosocomial bacteria.These bacteria develop or evolve with several antibiotic resistance mechanisms and therefore known to escape antibiotic... BACKGROUND Staphylococcus aureus(S.aureus)is one of the six highly pathogenic nosocomial bacteria.These bacteria develop or evolve with several antibiotic resistance mechanisms and therefore known to escape antibiotic treatments.Several reports suggest that multidrug resistance in S.aureus is increasing worldwide over the time.Indian population is comprised of diverse ethnic group residing different geographical regions.Recent reports revealed that antibiotic resistance in S.aureus isolates vary in different regions of India.AIM To identify antibiotic resistance pattern in S.aureus in India.METHODS In the present study,we overviewed resistance in S.aureus isolates to different antibiotics.We searched for the research articles on antibiotic resistance in S.aureus from India using Scopus,Google scholar,and PubMed databases(Prospero registration No.PROSPERO 2025CRD420251153034).We identified a total of 26 articles published during 2013 to 2024 in English language.We extracted the information about the location of the study,antibiotics used and percentage of the resistant isolates.RESULTS Among 26 studies,most of the studies were reported from northern India(31%),southern India(27%)and eastern India(27%),followed by western India(11%)and Pan India(4%).The findings highlight very high resistance to beta-lactam antibiotics,particularly penicillin and methicillin,reflecting a substantial burden of methicillinresistant S.aureus.Fluoroquinolones and macrolides also showed elevated resistance levels,whereas linezolid,teicoplanin,chloramphenicol,and doxycycline retained comparatively better activity.CONCLUSION The present review,demonstrated geographical region wise trends of antibiotic resistance in S.aureus from India,which will be useful in designing the further studies investigating antibiotic resistance patterns. 展开更多
关键词 Staphylococcus aureus Antibiotic resistance Geography India βlactam
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A microfluidic biosensor based on multiple signal amplification for rapid and sensitive detection of E.coli O157:H7 and Staphylococcus aureus 认领 引用
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作者 Xinrui Zhang Hui Chen +3 位作者 Tingting Fan Xueming Zhu Ying Tan Dan Gao 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第4期694-699,共6页
E.coli O157:H7 and Staphylococcus aureus have emerged as significant foodborne pathogens,characterized by considerable incidence rates and mortality.Despite advancements,current detection methods are hindered by chall... E.coli O157:H7 and Staphylococcus aureus have emerged as significant foodborne pathogens,characterized by considerable incidence rates and mortality.Despite advancements,current detection methods are hindered by challenges in enhancing specificity and sensitivity.Herein,we introduced a cutting-edge biosensor that employs a novel CHA-coupled CRISPR multi-stage signal amplification technique for the rapid and ultra-sensitive detection of these two pathogens.This microfluidic device consisted of an upstream serpentine mixing channel and a downstream boat-shaped microcavity equipped with a microcolumn array,facilitating efficient reagent mixing,robust CHA amplification,and CRISPR reactions.Multiple signal amplification was achieved through bacterial competitive binding triggered by catalytic hairpin assembly(CHA)and cr RNA-mediated CRISPR reactions.Based on this platform,the detection of target bacteria is transformed into nucleic acid detection,with a maximum detection range of 134 CFU/mL for E.coli O157:H7 and 181 CFU/mL for Staphylococcus aureus,which were better or comparable to previously reported biosensors.The entire assay was completed within approximately 1.5 h,with a minimal sample volume requirement of just 10μL.The biosensor exhibited a high recovery rate,ranging from 95%to 115%,and demonstrated excellent specificity towards the target bacteria.In summary,this biosensor offers a rapid,accurate,and highly sensitive tool for food safety and clinical diagnostics. 展开更多
关键词 Microfluidic chip Catalytic hairpin assembly CRISPR Staphylococcus aureus E.coli O157:H7
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Presence of Staphylococcus aureus amplifies zinc oxide nanoparticle toxicity in skin cells 认领 引用
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作者 Yuya Uehara Yuko Shimamura Shuichi Masuda 《Journal of Dermatologic Science and Cosmetic Technology》 2026年第1期99-108,共10页
Zinc oxide(ZnO)nanoparticles are widely used in topical products,including cosmetics and sunscreens,because of their ultraviolet-protective and antibacterial properties.Although ZnO nanoparticles are generally conside... Zinc oxide(ZnO)nanoparticles are widely used in topical products,including cosmetics and sunscreens,because of their ultraviolet-protective and antibacterial properties.Although ZnO nanoparticles are generally considered to have low toxicity in healthy skin,their safety profile remains uncertain because they can generate reactive oxygen species and induce oxidative stress.Staphylococcus aureus,a common skin commensal,can cause inflammation and barrier dysfunction,contributing to conditions such as atopic dermatitis.Despite the frequent use of ZnO nanoparticles and the ubiquitous presence of S.aureus on the skin,the health effects of their simultaneous exposure remain poorly understood.This study investigated the effects of ZnO nanoparticles on HaCaT keratinocytes when applied alone or in combination with S.aureus,focusing on oxidative stress,barrier integrity,inflammation,and apoptosis.ZnO nanoparticles induced oxidative stress,disrupted barrier integrity,triggered inflammatory responses,and promoted apoptosis associated with mitochondrial dysfunction.Co-exposure to ZnO nanoparticles and S.aureus further intensified these effects.Additional analyses revealed that activation of the mitogen-activated protein kinase pathway contributed to this enhanced response.These findings indicate that the biological impact of ZnO nanoparticles differs in the presence of skin microbiota compared with exposure alone.This study provides a revised perspective for evaluating the health risks of ZnO-based formulations and offers mechanistic insights useful for designing therapeutic strategies targeting S.aureus-associated skin disorders. 展开更多
关键词 Zinc oxide Staphylococcus aureus Co-exposure Oxidative stress Mitochondrial dysfunction
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MRSA生物膜相关ICD感染致急性脊髓炎与装置外露1例 认领 引用
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作者 于荣博 郭煊 +1 位作者 李发学 吴强 《中国循证心血管医学杂志》 2026年第7期876-879,共4页
植入式心脏复律除颤器(implantable cardioverter defibrillator,ICD)术后感染发生率为1%~3%,其中耐甲氧西林金黄色葡萄球菌(methicillin-resistant staphylococcus aureus,MRSA)是主要病原体之一。生物膜形成是此类感染难以治疗的核心... 植入式心脏复律除颤器(implantable cardioverter defibrillator,ICD)术后感染发生率为1%~3%,其中耐甲氧西林金黄色葡萄球菌(methicillin-resistant staphylococcus aureus,MRSA)是主要病原体之一。生物膜形成是此类感染难以治疗的核心机制,其通过胞外基质屏障阻碍抗生素渗透,并诱导细菌进入代谢休眠状态,导致感染迁延或全身播散。急性脊髓炎多与病毒感染或自身免疫相关,而细菌直接侵袭脊髓的病例较为罕见,目前鲜见ICD植入术后引发脊髓炎的病例报道。本病例揭示了生物膜相关MRSA感染向中枢神经系统扩散的潜在风险,为复杂感染的管理提供参考。 展开更多
关键词 装置外露 生物膜 MRSA 植入式心脏复律除颤器 ICD感染
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小檗碱联合庆大霉素治疗MRSA糖尿病足溃疡伤口感染的实验研究 认领 引用
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作者 周世利 刘倩 +3 位作者 李继红 轩芸 岳答 王少华 《河北医药》 CAS 2026年第6期941-944,949,共4页
目的探究小檗碱联合庆大霉素对耐甲氧西林金黄色葡萄球菌(methicillin-resistant staphylococcus aureus,MRSA)感染的糖尿病足溃疡伤口抑菌和愈合的效果。方法高脂高糖喂养建立2型糖尿病小鼠模型后采用背部全层皮肤损伤法建立糖尿病小... 目的探究小檗碱联合庆大霉素对耐甲氧西林金黄色葡萄球菌(methicillin-resistant staphylococcus aureus,MRSA)感染的糖尿病足溃疡伤口抑菌和愈合的效果。方法高脂高糖喂养建立2型糖尿病小鼠模型后采用背部全层皮肤损伤法建立糖尿病小鼠伤口,将20只小鼠分为4组:2型糖尿病小鼠伤口组(空白组)、伤口感染组(模型组)、庆大霉素组(对照组)和小檗碱联合庆大霉素组(观察组),每组5只。空白组为仅对2型糖尿病小鼠进行全层皮肤损伤,模型组为2型糖尿病小鼠进行全层皮肤损伤后涂抹MRSA建立糖尿病伤口感染模型,对照组为2型糖尿病小鼠伤口感染模型小鼠伤口湿敷庆大霉素治疗,观察组为2型糖尿病小鼠伤口感染模型小鼠伤口湿敷庆大霉素的同时以小檗碱灌胃治疗。观察小鼠伤口愈合情况和伤口菌落数。实验结束后,采用实时定量PCR方法检测小鼠伤口组织中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)、IL-1β水平。采用黄嘌呤氧化酶法(羟胺法)测定超氧化物歧化酶(SOD)活力;采用DTNB法测定谷胱甘肽(GSH)活力;采用比色法测定丙二醛(MDA)浓度。结果与对照组比较,观察组小鼠伤口显著愈合(P<0.05),炎性因子TNF-α、IL-6和IL-1β水平显著下降(P<0.05),SOD和GSH的水平均明显升高(P<0.05),MDA水平明显降低(P<0.05)。结论在2型糖尿病伤口感染模型中,小檗碱联合庆大霉素具有明显抑菌作用,可降低炎性反应和氧化应激水平的促进糖尿病足溃疡伤口愈合。 展开更多
关键词 耐甲氧西林金黄色葡萄球菌 糖尿病足溃疡 小檗碱 感染
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Pseudolaric Acid B-linked Double-network Hydrogel Alleviates Pruritus by Inhibiting The Growth of Staphylococcus aureus 认领 引用
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作者 YOU Ye YANG Yan +4 位作者 LI Tong-Yu CAI Cheng-Long WANG Ting ZHU Chan TANG Zong-Xiang 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2026年第6期1734-1745,共12页
Objective This study aimed to elucidate the mechanistic role of Staphylococcus aureus in the pathogenesis of atopic dermatitis(AD),a chronic inflammatory skin disorder characterized by pruritus and barrier dysfunction... Objective This study aimed to elucidate the mechanistic role of Staphylococcus aureus in the pathogenesis of atopic dermatitis(AD),a chronic inflammatory skin disorder characterized by pruritus and barrier dysfunction.A key focus was screening traditional Chinese medicine(TCM)active components with dual antibacterial and antipruritic efficacy,followed by systematic evaluation of their in vitro antibacterial activity.Additionally,a novel drug delivery system was constructed to enable localized efficient drug delivery,inhibiting S.aureus proliferation and alleviating its induced pruritus,thereby providing new strategies for targeted AD therapy.Methods Male C57BL/6J mice aged 6-8 weeks(body weight 18-22 g)were used to establish an AD model via repeated oxazolone sensitization.On day 14,microbial samples were collected from the lesional area(1 cm²)using sterile cotton swabs,followed by vortex mixing,serial dilution,and plating on 5%sheep blood agar plates(incubated at 37℃ for 24 h).Single colonies with complete transparentβ-hemolytic zones were isolated and identified as vancomycin-intermediate S.aureus(VISA)via 16S rRNA sequencing.An S.aureus mono-infection animal model was then established by applying gauze saturated with bacterial suspension(McFarland turbidity 0.1)to the nape and back skin of mice.The pruritic phenotype and inflammatory cell infiltration induced by S.aureus were evaluated using comprehensive approaches including behavioral assays(e.g.,scratching frequency recording),hematoxylin-eosin(HE)staining,and toluidine blue staining.The in vitro antibacterial efficacy of the TCM monomer pseudolaric acid B(PAB)and double network hydrogel(DN)was separately assessed by disk diffusion assay,while the minimum inhibitory concentration(MIC)of PAB was determined via broth dilution method.Further validation of the pharmacodynamic characteristics of the composite system(PAB@DN,composed of PAB and DN)was conducted through behavioral assays,HE staining,and dermatitis scoring,with its drug release profile evaluated by mass spectrometry analysis.Based on scratching behavioral analysis and dermatitis scoring,the optimal ratio and concentration of PAB@DN were optimized.Results The S.aureus load in AD lesional tissues was significantly higher than in normal skin((5.3±0.33)×10⁶CFU vs.(3.6±0.26)×10⁷CFU,P<0.001).In the S.aureus mono-infection group,mice exhibited a 6.7-fold increase in scratching frequency compared to the control group.HE staining revealed marked epidermal thickening((10.4±2.39)μm vs.(85.6±1.95)μm,P<0.0001),and toluidine blue staining showed a 23-fold increase in mast cell degranulation.Pseudolaric acid B exhibited a significant concentration-dependent inhibitory effect on S.aureus growth,with its in vitro antibacterial effect being 57%that of the antibiotic cefepime(inhibition zone diameter:PAB(1.885±0.036)cm vs.cefepime(3.636±0.005)cm,P<0.0001)and a minimum inhibitory concentration(MIC)of 1 g/L.The carrier double network hydrogel(DN)itself lacked direct antibacterial activity(no significant difference in inhibition zone diameter compared to the control)but effectively ameliorated the dry symptoms of AD-like lesions.The PAB@DN composite system demonstrated a synergistic effect compared to individual components,resulting in a 50%reduction in scratching behavior,an 86%decrease in dermatitis score,and a 60%reduction in epidermal thickening.It also reduced the S.aureus load in mouse skin by approximately 34%,with the optimal effective formulation being PAB at 1 g/L loaded onto DN.Conclusion S.aureus colonization plays a critical driving role in the onset and progression of AD.Using an S.aureus infection model,this study confirmed that the pseudolaric acid B-hydrogel composite delivery system(PAB@DN)can effectively alleviate S.aureus-induced pruritus and skin damage,providing experimental evidence for microbiota-targeted therapy of AD. 展开更多
关键词 atopic dermatitis pruritus Staphylococcus aureus pseudolaric acid B double-network hydrogel
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胍乙啶对兔MRSA眼内炎的预防保护作用 认领 引用
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作者 梁倩 邵征 +2 位作者 樊峥 王维娜 黄钰森 《中华实验眼科杂志（中英文）》 CAS CSCD 北大核心 2026年第7期628-636,共9页
目的建立兔耐甲氧西林金黄色葡萄球菌(MRSA)眼内炎模型并探究交感神经抑制剂胍乙啶对眼内炎炎症反应的调控作用。方法选取2.5 kg左右雄性新西兰白兔6只,玻璃体内分别注射1.5×104、1.5×105菌落形成单位(CFU)MRSA细菌悬液... 目的建立兔耐甲氧西林金黄色葡萄球菌(MRSA)眼内炎模型并探究交感神经抑制剂胍乙啶对眼内炎炎症反应的调控作用。方法选取2.5 kg左右雄性新西兰白兔6只,玻璃体内分别注射1.5×104、1.5×105菌落形成单位(CFU)MRSA细菌悬液50 μl,每组3只,筛选MRSA眼内炎模型的稳定感染浓度。另取新西兰白兔36只,采用随机数表法将其分为对照组、地塞米松组、胍乙啶组和预防用药组,每组9只。其中对照组、地塞米松组、胍乙啶组单眼建模1 d后玻璃体内分别注射无菌生理盐水、4 mg/ml地塞米松磷酸钠注射液、5 mg/ml胍乙啶50 μl,预防用药组建模前1 h玻璃体内注射5 mg/ml胍乙啶50 μl,后续用药同胍乙啶组,从成功建模后第1天开始隔天分组进行药物治疗。建模后第1、3、5、7、9、11天,采用裂隙灯显微镜观察各组炎症反应并进行炎症评分。建模后第1、3、5天,采用标准梯度稀释法和细菌平板计数法测定各组眼内细菌载量。建模后第5天,采用苏木精-伊红染色法观察各组眼组织结构。采用胍乙啶体外抑菌实验观察各组MRSA生长情况。结果兔眼玻璃体内注射1.5×104 CFU MRSA可得到表型稳定的MRSA眼内炎模型。建模后对照组炎症持续进展,角膜混浊,角膜血管翳形成,眼底红光反射消失形成黄色混浊;地塞米松组眼前节炎症较轻,后段炎症逐渐进展,红光反射消失;胍乙啶组虹膜血管持续充血膨隆;预防用药组炎症最轻,角膜清澈透明,红光反射良好。各组建模后不同时间点炎症评分总体比较差异有统计学意义(F组别=20.72,P<0.001;F时间=9.66,P<0.001),其中预防用药组第3、9、11天炎症评分均小于相应时间点对照组、地塞米松组和胍乙啶组,预防用药组第7天炎症评分小于相应时间点对照组,地塞米松组和胍乙啶组第9天炎症评分均小于相应时间点对照组,差异均有统计学意义(均P<0.05);与组内第1天相比,对照组第7、9、11天炎症评分均显著上升,地塞米松组第3、11天炎症评分显著升高,胍乙啶组仅第11天炎症评分升高,差异均有统计学意义(均P<0.05)。建模后第1、3、5天,对照组、地塞米松组、胍乙啶组均出现大量金黄色菌落,预防用药组无菌落生长。各组建模后不同时间点CFU总体比较差异有统计学意义(F组别=15.73,P<0.001;F时间=9.45,P<0.001),其中预防用药组第3、5天CFU均小于相应时间点地塞米松组,地塞米松组第5天CFU均大于相应时间点对照组和胍乙啶组,差异均有统计学意义(均P<0.05);地塞米松组建模后CFU持续上升,第1、3、5天CFU两两比较差异均有统计学意义(均P<0.05)。建模后第5天,对照组和地塞米松组可见大量炎性渗出及炎症细胞浸润,视网膜结构破坏严重。胍乙啶组炎症细胞浸润及渗出减少,视网膜各分层依稀可见。预防用药组视网膜结构完整,未见明显渗出和炎症细胞浸润。胍乙啶体外抑菌实验中,对照组MRSA于6 h开始进入对数生长期,16 h达高峰。胍乙啶组细菌生长情况和头孢呋辛组相似,细菌悬液吸光度值始终处于较低水平,位于0附近。结论预防使用交感神经抑制剂胍乙啶可有效减轻MRSA感染后的眼内炎症反应、炎症细胞浸润及视网膜组织结构损伤。 展开更多
关键词 眼内炎,细菌性 耐甲氧西林金黄色葡萄球菌 炎症反应 交感神经抑制剂 胍乙啶
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小鼠MRSA慢性假体周围感染诱导成熟三级淋巴结构形成及骨溶解 认领 引用
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作者 木尼拉·麦合木提 黄利思 +7 位作者 李家楷 蔡如龙 冯棋 林于迪 黄泽泓 伍思佳 Muhammad Umar 方航 《南方医科大学学报》 CAS CSCD 北大核心 2026年第7期1541-1550,共10页
目的探讨耐甲氧西林金黄色葡萄球菌(MRSA)诱导的小鼠膝关节假体周围感染(PJI)模型中三级淋巴结构(TLS)的结构与功能特征及其与局部骨溶解的关系。方法构建MRSA诱导的小鼠膝关节PJI模型,将C57BL/6J小鼠随机分为假体组(PBS)和PJI组(1×... 目的探讨耐甲氧西林金黄色葡萄球菌(MRSA)诱导的小鼠膝关节假体周围感染(PJI)模型中三级淋巴结构(TLS)的结构与功能特征及其与局部骨溶解的关系。方法构建MRSA诱导的小鼠膝关节PJI模型,将C57BL/6J小鼠随机分为假体组(PBS)和PJI组(1×10~4CFU MRSA USA300),观察60 d。检测外周血白细胞计数(WBC)、红细胞沉降率(ESR)及C反应蛋白(CRP)等炎症指标;HE染色评估假体周围组织TLS形成及成熟程度;多重免疫荧光分析T/B细胞分区(CD3/B220)、滤泡树突状细胞(CD21)及TLS功能活性(Ki67、PNAd);TRAP染色进行骨形态计量学分析,评估破骨细胞数量(N.Oc/BS)。结果术后21d,PJI组外周血炎症指标显著高于假体组(P<0.0001)。组织学显示,PJI组假体周围形成大量成熟TLS,截面积达17212.10±7624.28μm2,显著高于假体组(P<0.01)。多重免疫荧光证实PJI组呈现“T细胞环绕B细胞”的成熟TLS典型空间结构,并伴有FDC网络;TLS内存在大量Ki67阳性增殖细胞,边缘及实质内可见PNAd阳性高内皮微静脉。TRAP分析显示,PJI组单位骨表面破骨细胞数量为(28.75±4.13)/mm,显著高于假体组的(1.63±0.74)/mm(P<0.0001)。结论MRSA慢性感染可在小鼠假体周围诱导形成具备典型结构分区及功能活性的成熟TLS,并伴随显著骨溶解。 展开更多
关键词 耐甲氧西林金黄色葡萄球菌 假体周围感染 三级淋巴结构 小鼠模型 免疫微环境
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