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Bacillus velezensis activates beneficial soil microorganisms to promote the growth and health of cucumber after soil fumigation 认领 引用 被引量：2
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作者 Xuewen Xie Lida Chen +8 位作者 Shicheng Liu Lixia Yan Na Li Changzong Ding Yanxia Shi Ali Chai Tengfei Fan Baoju Li Lei Li 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第6期1403-1415,共13页
Cucumber root rot,primarily caused by Fusarium solani,threatens global cucumber production through soil-borne infection,vascular wilt,and yield loss.Soil fumigation with Calcium cyanamide(CaCN2)effectively suppress... Cucumber root rot,primarily caused by Fusarium solani,threatens global cucumber production through soil-borne infection,vascular wilt,and yield loss.Soil fumigation with Calcium cyanamide(CaCN2)effectively suppresses soil-borne pathogens but may impair beneficial soil microbiota.This study investigated the synergistic effects of Bacillus velezensis(ZF336)inoculation on reactivating beneficial soil microbes after CaCN2 fumigation and its role in controlling cucumber root rot caused by F.solani.The physicochemical properties and microbial community structure of the soil and the health of the cucumber plants were investigated.Our findings revealed the following.(Ⅰ)The potential for hydrogen(pH)and the contents of organic matter(OM),nitrate nitrogen(NO3-N)and ammonium nitrogen(NH4-N)significantly increased after combined CaCN2 and ZF336 treatment.(Ⅱ)Combined CaCN2 and ZF336 treatment reduced the relative abundance of Fusarium and increased the relative abundances of the potentially beneficial bacteria Trichoderma and Penicillium in the soil.In addition,B.velezensis promoted the recovery of microbial communities after CaCN2 disinfection.(Ⅲ)Combined CaCN2 and ZF336 treatment improved the activities of carbohydrate enzymes,effectively promoting soil carbon metabolism and increasing the cellulase,hemicellulose and lignin decomposition abilities.In conclusion,CaCN2 combined with B.velezensis stimulated the soil microbial community structure and the activities of specific enzymes and is an effective way to control cucumber root rot disease and improve yield and soil quality. 展开更多
关键词 Cucumber Calcium cyanamide Bacillus velezensis Soil microbial community Function genes Fusarium
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Effect of Mixed Inoculation of Bacillus amyloliquefaciens and Bacillus cereus on the Quality of High-Temperature Daqu 认领 引用
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作者 YUAN Siqi HE Qi +6 位作者 ZHAO Jinsong ZHANG Yadong YIN Jiebing LIU Xun LIU Yufeng SHEN Yi LIU Jun 《食品科学》 EI CAS CSCD 北大核心 2026年第7期143-158,共16页
This study investigated the effects of different methods of Bacillus inoculation(separate cultures,co-culture,and no inoculation)on the sensory characteristics and flavor pyrazine compounds of high-temperature Daqu.Du... This study investigated the effects of different methods of Bacillus inoculation(separate cultures,co-culture,and no inoculation)on the sensory characteristics and flavor pyrazine compounds of high-temperature Daqu.During the fermentation process,total acidity and moisture content decreased progressively,and sensory attributes of Bacillusfortified Daqu were superior to those of the control group.For finished Daqu,the total content of pyrazine compounds was significantly higher in the experimental groups(3376.89μg/g for fortified Daqu II,2842.47μg/g for fortified Daqu I)than in the control group(1868.78μg/g).A total of 24 prokaryotic and 16 eukaryotic genera were identified as dominant microbiota.The abundance of Bacillus,Thermomyces,and Thermoascus in fortified Daqu was higher than that in the control group.A total of 21 prokaryotic and 19 eukaryotic differentially abundant microorganisms were identified.In summary,the inoculation of Bacillus seed culture contributed to improved sensory attributes,enhanced microbial diversity to some extent,and increased the concentration of pyrazine compounds,thereby improving the overall quality of high-temperature Daqu. 展开更多
关键词 fortified Daqu Bacillus physicochemical indicators microbial community Jiangxiangxing Baijiu pyrazine compounds
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Deciphering the potential of Bacillus cereus HS-9 in cadmium bioremediation and ensuring rice safety 认领 引用
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作者 Mengmeng Wang Jiayin Zhao +9 位作者 Jianan Gao Sibo Cai Yongjing Gu Yu Liu Li Gao Yu Xu Yuncheng Wu Zhengtong Zhou Jibing Zhang Wei Tian 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第4期573-583,共11页
Cadmium(Cd)contamination in agricultural soils poses significant environmental and health risks due to its nondegradable and bio-magnifying nature.With the global imperative for eco-friendly Cd remediation strategies,... Cadmium(Cd)contamination in agricultural soils poses significant environmental and health risks due to its nondegradable and bio-magnifying nature.With the global imperative for eco-friendly Cd remediation strategies,microbial bioremediation emerges as a promising approach.Here,Bacillus cereus HS-9 was isolated from Cdcontaminated paddy soil using LB medium supplemented with 5 mg/L of Cd.HS-9 exhibited an impressive Cd removal efficiency of 95.44%at a concentration of 5 mg/L.A rice pot experiment was conducted using Cdcontaminated soil,with HS-9 inoculation as the treatment group and non-inoculated soil as the control.The treatment group resulted in a 38.99%reduction in soil Cd availability and a 34.33%decrease in rice Cd content without affecting rice yield.The microbial community of the rice rhizosphere was analyzed using metagenome sequencing.The results revealed an increased abundance of czcA,frnE,and irlS genes in the soil microbiome,indicating enhanced Cd resistance and efflux capabilities.Microbial community showed significant shifts towards a beneficial microbial consortium,particularly marked by increases in Lysobacter and Sphingomonas genera which are known for their roles in heavy metal resistance and bioremediation.B.cereus HS-9 demonstrated significant potential for the bioremediation of Cd-contaminated soil.This study provides foundation for the development of microbial-based strategies for the eco-friendly remediation of heavy metal-polluted agricultural lands. 展开更多
关键词 Cd contamination Bioremediation Bacillus cereus HS-9 Soil microbiome Rice safety
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Bacillus subtilis enhances maize yield by restricting cadmium translocation and modulating ion homeostasis 认领 引用
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作者 Haseeb Ahmad Jiu Yang Mao +7 位作者 Rayyan Khan Muhammad Ali Shah Kashif Khan Muhammad Asad Naseer Shahid Ali Rida Rashid Rui He Xun Bo Zhou 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第5期311-327,共17页
Cadmium(Cd)pollution in soils severely impacts maize growth.This study investigates the potential application of Bacillus subtilis to soil(BSS)as a bioremediator to mitigate Cd-induced stress during the V8 and VT grow... Cadmium(Cd)pollution in soils severely impacts maize growth.This study investigates the potential application of Bacillus subtilis to soil(BSS)as a bioremediator to mitigate Cd-induced stress during the V8 and VT growth stages of maize.The effects of B.subtilis on maize growth,photosynthesis,antioxidant defense,cellular ultrastructure,osmo-regulatory substances,ion homeostasis,and yield under Cd stress were evaluated.Cd exposure(Cd25 and Cd50 mg/kg)significantly reduced plant height,shoot biomass,leaf area,chlorophyll content,and photosynthetic efficiency by 8%-47%while increasing oxidative stress markers(superoxide anion(O2•-),hydrogen peroxide(H2O2),malondialdehyde(MDA))up to 3-fold.Co-exposure of B.subtilis with Cd stress(Cd25BSS and Cd50BSS)improved these parameters,enhancing plant growth and photosynthetic performance by 7%-40%and reduced stress biomarkers by 8%-38%.Cd25BSS also enhanced superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbate peroxidase(APX),and glutathione reductase(GR)by 12%-34%and upregulated antioxidant genes(ZmSOD1,ZmPOD4,ZmCAT1,ZmAPX1,and ZmGR1)compared to Cd25.Additionally,Cd25BSS increased osmotic regulation through higher soluble sugars and proteins,contributing to cellular stability under stress.Ion homeostasis was improved by increasing essential nutrients from 6%-70%while reducing Cd accumulation by 25%-85%in different maize tissues at both growth stages.Moreover,yield exhibited a strong positive correlation with growth parameters and essential ions(nitrogen,phosphorous,potassium,iron,and zinc),while showing negative correlation with MDA and Cd accumulation.This study highlights B.subtilis as an effective strategy for mitigating Cd stress,improving maize productivity in contaminated soils,and supporting sustainable agricultural practices. 展开更多
关键词 Cadmium Bacillus subtilis Maize Antioxidant defense Ionome Yield
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解磷菌Bacillus vallismortis gz4-1生物有机肥制备工艺优化 认领 引用 被引量：1
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作者 赵佳 王晓勇 +2 位作者 张轶珺 裴红宾 高振峰 《现代农业科技》 2026年第6期150-154,共5页
本文以解磷菌Bacillus vallismortis gz4-1为研究对象,制备新型解磷菌生物有机肥,以提高肥料性能,并减少磷肥使用量。采用单因素试验和响应面Box-Behnken试验相结合的方法对解磷菌B.vallismortis gz4-1生物有机肥配方及发酵工艺进行了优... 本文以解磷菌Bacillus vallismortis gz4-1为研究对象,制备新型解磷菌生物有机肥,以提高肥料性能,并减少磷肥使用量。采用单因素试验和响应面Box-Behnken试验相结合的方法对解磷菌B.vallismortis gz4-1生物有机肥配方及发酵工艺进行了优化,并利用钼锑抗比色法分析了生物有机肥制备过程中可溶性磷含量变化。结果表明:响应面Box-Behnken试验优化后,优选基质配方为小麦秸秆∶菜籽饼∶腐熟羊粪肥∶麦麸=37∶6∶10∶5(w/w);解磷菌B.vallismortis gz4-1生物有机肥发酵优选条件为接种量1%(v/w)、菌液浓度1.0×106CFU/mL、发酵温度30℃、发酵时间11 d,且在此条件下,发酵制成的解磷菌生物有机肥活菌数可达0.344×108CFU/g,可溶性磷含量为80.21 mg/kg,符合《生物有机肥》(NY 884—2012)标准中规定的活菌数量要求。本试验条件下制备的解磷菌生物有机肥,为提升磷肥利用率、减少化学磷肥投入提供了新方案,其基质配方与发酵工艺参数可为同类型菌肥开发提供参考依据。 展开更多
关键词 有机磷降解菌 Bacillus vallismortis gz4-1 生物有机肥 基质配方 发酵工艺
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Insight into the inactivation of Bacillus cereus spores by ohmic heating and application in liquid food 认领 引用
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作者 Yingying Sun Yana Liu +5 位作者 Fei Jia Han Wang Yuhan Wang Yi Liu Xingmin Li Ruitong Dai 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第5期2273-2284,共12页
Bacillus cereus spores pose a potential threat to various food products,including meat,grain,vegetables,and dairy products,due to their remarkable resistance to environmental stress.Ohmic heating(OH)is an emerging alt... Bacillus cereus spores pose a potential threat to various food products,including meat,grain,vegetables,and dairy products,due to their remarkable resistance to environmental stress.Ohmic heating(OH)is an emerging alternative technology for inactivating spores.This study aimed to investigate the effects of OH on B.cereus spores,specifically examining its impact on heat resistance,leakage of intracellular substances,integrity of the cortex and membrane,and structural changes,in order to elucidate the potential inactivation mechanism of OH.The results demonstrated the efficacy of OH in inactivating B.cereus spores in various liquid food products while maintaining product quality.The wet heat resistance of spores decreased after OH treatments at low-temperatures(60-70℃),potentially promoting subsequent inactivation.Conversely,resistance increased following OH treatments at higher temperatures and oil bath heating(OB)treatments.Similar inactivation effects were observed between OB and OH when the temperature reached 80 and 98℃.Both OH and OB treatments damaged spore structures,resulting in leakage of intracellular substances and disruption of homeostasis.However,OH treatment at 10 V/cm and 50 Hz resulted in less leakage of dipicolinic acid,protein,nucleic acids within the spores,and appeared to have additional effects on the structures within the inner membrane.These findings could facilitate the development of OH as a promising technology for effective spore control in food. 展开更多
关键词 Bacillus cereus spores Thermal-electrical technique Heat resistance Structure Intracellular substances
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The potential of metabolites released by Bacillus subtilis CH21 for the effective control of the harmful cyanobacterium Raphidiopsis raciborskii 认领 引用
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作者 Wei Zhang Xu Zeng +4 位作者 Jiayi Dong Yibin Su Erik Jeppesen Liqing Wang Sicheng Yin 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第6期572-580,共9页
Microorganism-based biocontrol strategies for harmful cyanobacterial blooms have gained increasing attention due to their ecological compatibility and species-specific targeting.Despite this,research on algicidal bact... Microorganism-based biocontrol strategies for harmful cyanobacterial blooms have gained increasing attention due to their ecological compatibility and species-specific targeting.Despite this,research on algicidal bacteria effectiveness against the invasive cyanobacterium Raphidiopsis raciborskii remains limited.Research has been conducted on the Bacillus subtilis strain CH21,which was isolated from Chaohu Lake in China.exhibiting potent indirect anticyanobacterial activity against R.raciborskii.Within 48 h of exposure,the cell-free filtrate of strain CH21 achieved 96.2%removal efficiency of R.raciborskii cells.Physiological and morphological analyses revealed severe disruption of the photosynthetic apparatus(Fv/Fm and Yield values reduced to zero within 16 h),oxidative stress(elevation in malondialdehyde content and reactive oxygen species,reduction in superoxide dismutase and catalase activity),and cellular damage,including pseudovacuole collapse,the degradation of polyphosphate granules and cytoplasmic leakage.Metabolomic profiling identified surfactin-class lipopeptides as putative bioactive agents,which demonstrated exceptional stability across a wide thermal(25–121°C)and pH(3–13)range.These findings highlight B.subtilis CH21 as a promising candidate for targeted biocontrol of R.raciborskii blooms,supporting the development of environmentally mitigation technologies to be used in cases where it is difficult to control R.raciborskii by external nutrient loading reduction or if the response to such loading reductions are slow. 展开更多
关键词 Harmful cyanobacterial blooms Algicidal bacteria Raphidiopsis raciborskii Bacillus subtilis
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芽孢杆菌Bacillus sp.Z2的筛选及其在甜香型香料制备中的应用 认领 引用
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作者 杨宗灿 张新龙 +5 位作者 杨金初 赵森森 王秋领 冯颖杰 黄申 毛多斌 《陕西科技大学学报》 CAS 北大核心 2026年第2期76-83,92,共8页
为开发甜香型烟草香料,从新鲜烟叶表面中筛选发酵烟草提取液使其感官质量增甜的菌株,优化发酵条件,冷萃制备甜香型烟草香料,分析香味成分和加香应用效果,利用非靶向代谢组分析发酵菌株的代谢通路.结果显示:筛选获得增甜香菌株枯草芽孢杆... 为开发甜香型烟草香料,从新鲜烟叶表面中筛选发酵烟草提取液使其感官质量增甜的菌株,优化发酵条件,冷萃制备甜香型烟草香料,分析香味成分和加香应用效果,利用非靶向代谢组分析发酵菌株的代谢通路.结果显示:筛选获得增甜香菌株枯草芽孢杆菌(Bacillus sp.Z2),优化确定最佳发酵参数为温度33℃、时间24 h、烟草提取液浓度13.0°Bé;发酵后巨豆三烯酮a和2-乙酰基吡咯含量分别提升417%(55.9 ug/mL)和175%(12.73 ug/mL),5-羟甲基糠醛含量显著增加(123.38 ug/mL),总香气成分增幅达329%(236.08 ug/mL);加香感官评价显示甜香特征强化且香气品质提升;非靶向代谢组学分析表明烟酸和6-羟基烟酸等代谢通路显著上调.本研究经微生物发酵,获得了一种具有甜香特征的烟草提取液,为开发特征香韵的烟草香料提供了借鉴. 展开更多
关键词 烟草提取液 甜香型香料 GC-MS 增香菌株Bacillus sp.Z2 非靶向代谢组学
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Enhancing the biodegradation of low-density polyethylene(LDPE)using novel bacterial consortia:Bacillus sp.AS3 and Sphingobacterium sp.AS8 认领 引用 被引量：2
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作者 Pornya Khampratueng Anil Kumar Anal 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第1期263-270,共8页
Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,red... Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,reducing hydrophobicity and decreasing tensile strength.Additionally,pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film.AS3–8 consortia exhibited a maximum weight loss of 8.0%±0.5%after 45 days of incubation.While Bacillus sp.AS3 and Sphingobacterium sp.AS8 demonstrated LDPE weight loss of 5.03%±1.6%and 1.6%±0.5%,respectively.The structure of LDPE was altered after incubation with the bacterial strains,resulting in a reduction in the intensity of functional groups,including C=O,C=C,N–H,and C–N.The carbonyl index(CI)of LDPE also decreased by 7.17%after the consortia AS3–8 degradation.Consortia AS3–8 significantly impacted the physical properties of LDPE by reducing the water contact angle(WCA),decreasing to 64.21°±3.69°,and tensile strength(TS),decreasing to 17.97±0.3 MPa.Moreover,the esterase activity was measured through 45 days of incubation.SDS-PAGE analysis of the AS3–8 consortia revealed bands at 35,48,and 70 kDa molecular weights,similar to known enzymes like laccase and esterase.Furthermore,SEM observations showed rough,cracked surfaces on pretreated LDPE,with biofilms present after incubation with the bacterial strains.GC–MS analysis revealed that AS3–8 consortia produced depolymerized chemicals,including alkanes,aldehydes,and esters.The LDPE biodegradation pathway was elucidated.This study addresses critical knowledge gaps in improving plastic degradation efficiency. 展开更多
关键词 Biodegradation Low-density polyethylene Bacillus sp. Sphingobacterium sp.
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Dietary supplementation with Bacillus-based probiotic improves gut health in the weaned piglets challenged by rotavirus 认领 引用
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作者 Fengyu Xiang Heng Yang +15 位作者 Xiangqi Fan Dayan Tan Bing Huang Bing Yu Jun He Yuheng Luo Junqiu Luo Hui Yan Junning Pu Jianping Wang Quyuan Wang Huifen Wang John Kyaw Htoo Santa Maria Mendoza Guiling Yan Xiangbing Mao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期951-961,共11页
Background As probiotics,Bacillus strains may regulate some physiological functions in animals.This study aimed to evaluate whether dietary supplementation with a Bacillus-based probiotic could alleviate gut damage in... Background As probiotics,Bacillus strains may regulate some physiological functions in animals.This study aimed to evaluate whether dietary supplementation with a Bacillus-based probiotic could alleviate gut damage induced by rotavirus(RV)infection in piglets.Twenty-four piglets were randomly assigned into 2 groups fed with the basal diet(n=16)and the diet containing 109 colony-forming unit Bacillus spores/kg(n=8).On d 8,8 piglets fed with the diet supplemented with Bacillus-based probiotic and 8 piglets fed with basal diet were orally infused with RV,while the residue piglets had oral gavage of sterile essential medium.The trial duration was 12 d.Results RV challenge induced diarrhea,significantly destroyed the morphology of jejunal mucosa(P<0.05),significantly increased RV-antibody and RV non-structural protein 4 of jejunal mucosa(P<0.05),significantly impaired antioxidant capacity(including malondialdehyde level,total antioxidant capacity and catalase activity),immunity(such as interleukin 2,interleukin 4 and secreted immunoglobulin A levels),mucins and the m RNA expression of tightjunction-related(such as Zonula occludens 1,occludin)and apoptotic-related(including B-cell lymphoma/leukaemia-2-associated X protein,B cell lymphoma/leukaemia-2,cysteinyl aspartate specific proteinases)genes of jejunal mucosa(P<0.05),and,to some extents,affected the bacteria community structure and abundance of ileal digesta in piglets.However,Bacillus-based probiotic administration could significantly attenuate the negative effects of RV infection on gut health of piglets(P<0.05).Conclusions These findings suggested that supplementing Bacillus-based probiotic in the diet could decrease diarrhea rate,and improve gut health in weaned piglets,which was associated with regulating intestinal antioxidant capacity,apoptosis,and microbiota. 展开更多
关键词 Apoptosis Antioxidant capacity Bacillus strains Intestinal health Microbiota Rotavirus Weaned piglets
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Phased Zero-Aquatic System Exchange in Shrimp Farming:A Farming Method Combining Sodium Humate and Bacillus 认领 引用
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作者 GAO Shan LIAO Wuquan +4 位作者 CUI Yuhang LIN Zexu SHI Gang WANG Ping SUN Chengbo 《Journal of Ocean University of China》 SCIE CAS CSCD 2026年第1期237-246,共10页
The conservation of aquatic systems is closely linked to the maintenance and improvement of aquaculture products'yield and quality.In this experiment,a high-quality aquatic system was established,comprising Litope... The conservation of aquatic systems is closely linked to the maintenance and improvement of aquaculture products'yield and quality.In this experiment,a high-quality aquatic system was established,comprising Litopenaeus vannamei as a model species and two strains of Bacillus(W1 and XYB4)combined with sodium humate under zero-water exchange conditions.The growth performance,enzyme activity,and aquatic system microbial environment of L.vannamei were analyzed.Results showed that the combination of sodium humate and Bacillus strains effectively enhanced environmental conditions for the growth and reproduction of heterotrophic bacteria while inhibiting the growth of Vibrio species,including green and yellow variants.Microbiome analysis showed that the group treated with Bacillus strains combined with sodium humate exhibited significantly higher relative abundances of Firmicutes and Actinobacteriota than the other groups.Correspondingly,this treatment group showed substantially enhanced weight gain rate,specific growth rate,survival rate,and feed coefficient.Moreover,the phenol oxidase,catalase,lysozyme,and superoxide dismutase indexes of shrimps subjected to Bacillus–sodium humate treatment were considerably higher than those of the control group.These findings confirm that the combination of Bacillus and sodium humate has beneficial effects on shrimp growth and aquatic system quality control,providing a promising strategy for enhancing the efficiency of shrimp farming and aquaculture. 展开更多
关键词 Litopenaeus vannamei zero-aquatic system exchange Bacillus sodium humate microbial environment
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Advancing living Bacillus spore identification:Multi-head self-attention mechanism-enabled deep learning combined with single-cell Raman spectroscopy 认领 引用
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作者 Mengjiao Xue Fusheng Du +5 位作者 Lin He Junhui Hu Yuanpeng Li Yuan Lu Shuwen Zeng Yufeng Yuan 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2026年第1期139-155,共17页
Many spore-forming Bacillus species can cause serious human diseases,because of accidental Bacillusspore infection.Thus,developing an identification strategy with both high sensitivity and specificity is greatly in de... Many spore-forming Bacillus species can cause serious human diseases,because of accidental Bacillusspore infection.Thus,developing an identification strategy with both high sensitivity and specificity is greatly in demand.In this work,we proposed a novel approach named multi-head self-attention mechanism-guided neural network Raman platform to identify living Bacillus spores within a single-cell resolution.The multi-head self-attention mechanism-guided neural network Raman platform was created by combining single-cell Raman spectroscopy,convolutional neural network(CNN),and multi-head self-attention mechanism.To address the limited size of the original spectra dataset,Gaussian noise-based spectra augmentation was employed to increase the number of single-cell Raman spectra datasets for CNN training.Owing to the assistance of both spectra augmentation and multi-head self-attention mechanism,the obtained prediction accuracy of five Bacillus spore species was further improved from 92.29±0.82%to 99.43±0.15%.To figure out the spectra differences covered by the multi-head self-attention mechanism-guided CNN,the relative classification weight from typical Raman bands was visualized via multi-head self-attention mechanism curve.In the process of spectra augmentation from 0 to 1000,the distribution of relative classification weight varied from a discrete state to a more concentrated phase.More importantly,these highlighted four Raman bands(1017,1449,1576,and 1660 cm-1)were assigned large weights,showing that the spectra differences in the Raman bands produced the largest contribution to prediction accuracy.It can be foreseen that,our proposed sorting platform has great potential in accurately identifying Bacillus and its related genera species at a single-cell level. 展开更多
关键词 Multi-head self-attention mechanism CNN single-cell Raman spectroscopy spectra augmentation advanced Bacillus spore identification
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Biocontrol efficiency and the functional mechanisms of Bacillus velezensis RT-30 against kiwifruit soft rot 认领 引用
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作者 Wenli Yue Junru Xu +4 位作者 Zitao Lv Chen Zhou Shunyuan Wu Yunliu Zeng Pu Liu 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第5期1066-1076,共11页
Soft rot is a severe postharvest disease affecting kiwifruit production.The management of this disease primarily relies on the use of chemical fungicides.However,biological control using antagonistic microorganisms pr... Soft rot is a severe postharvest disease affecting kiwifruit production.The management of this disease primarily relies on the use of chemical fungicides.However,biological control using antagonistic microorganisms presents a promising alternative to fungicides.In the present study,whole-genome sequencing identified and characterized Bacillus velezensis RT-30,as a biocontrol strain.The anti SMASH(antibiotics&Secondary Metabolite Analysis Shell)analysis was employed to predict the possible secondary metabolites produced by strain RT-30.In vitro tests demonstrated that RT-30 inhibited the growth of both Botryosphaeria dothidea and Diaporthe phragmitis,while in vivo tests using Actinidia chinensis var.deliciosa'Xuxiang'and'Cuixiang'varieties also revealed a significant control effect.Molecular sequence analysis and Ultra-high pressure liquid chromatography-mass spectrometry(UPLC-MS)analysis of the fermentation filtrate of RT-30 indicated that the biocontrol strain primarily secreted three types of antimicrobial substances:macrolactin,bacillaene,and mycosubtilin.Furthermore,we explored the cultivation conditions of RT-30,recognizing the potential of this versatile bacterium for applications beyond antimicrobial production.This study provides a valuable biological tool for the management of kiwifruit soft rot and deepens our understanding of the biocontrol mechanism of B.velezensis. 展开更多
关键词 Kiwifruit Botryosphaeria dothidea Postharvest disease Bacillus velezensis RT-30 Biological control
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Impact of dietary supplementation of Bacillus subtilis on the metabolic profiles and microbial ecology of weanling pigs experimentally infected with a pathogenic Escherichia coli 认领 引用
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作者 Kwangwook Kim Sangwoo Park +2 位作者 Cynthia Jinno Peng Ji Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期1020-1033,共14页
Background Our previous study demonstrated that dietary supplementation of Bacillus subtilis enhanced growth performance and intestinal integrity in weaned pigs challenged with enterotoxigenic Escherichia coli(ETEC).T... Background Our previous study demonstrated that dietary supplementation of Bacillus subtilis enhanced growth performance and intestinal integrity in weaned pigs challenged with enterotoxigenic Escherichia coli(ETEC).Therefore,this study aimed to explore the impact of Bacillus subtilis on gut health and its role in modulating host–microbe interactions in post-weaning pigs.Results ETEC infection disrupted key metabolic pathways in distal colon,including glutathione,beta-alanine,and pyrimidine metabolism,indicating increased oxidative stress,impaired nucleotide balance,and amino acid catabolic stress.Bacillus subtilis supplementation induced distinct metabolomic and microbiome profiles in colon digesta of weaned pigs challenged with ETEC.Bacillus subtilis-treated pigs under ETEC challenge exhibited significant enrichment in amino acid-and energy-related pathways such as arginine biosynthesis,phenylalanine metabolism,pantothenate and CoA biosynthesis.ETEC infection induced microbial dysbiosis in the distal colon,resulting in decrease(P<0.05)in abundance of Streptococcaceae and Enterobacteriaceae compared to healthy controls.Bacillus subtilis supplementation mitigated the ETEC-induced disruptions by increasing the relative abundance of beneficial bacterial families,including Lachnospiraceae and Bacteroidaceae.Conclusion Supplementation of Bacillus subtilis improves intestinal health and resilience against ETEC challenge by mitigating infection-induced metabolic disruptions and gut dysbiosis in weaned pigs. 展开更多
关键词 Bacillus subtilis Colon microbiota Enterotoxigenic Escherichia coli F18 Metabolomics Weaned pigs
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Growth and Yield Responses of Soledad Chili Pepper(Capsicum annuum L.)to the Application of Chitosan and Bacillus subtilis 认领 引用
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作者 Adolfo Amador Mendoza Rosalba Guadalupe Gomez Raymundo +5 位作者 Ana Rosa Ramírez Seañez Hipolito Hernández Hernández Rogelio Enrrique Palacios Torres Nelda Xanath Martínez Galero Miguelángel García Muñoz Saribel Zilli Gutiérrez 《Phyton-International Journal of Experimental Botany》 SCIE 2026年第1期151-186,共36页
The cultivation of Soledad pepper(Capsicum annuum L.)is essential in Oaxaca and Veracruz,but it faces issues with pests and diseases,which affect yield and cause economic losses.To mitigate these impacts,farmers have ... The cultivation of Soledad pepper(Capsicum annuum L.)is essential in Oaxaca and Veracruz,but it faces issues with pests and diseases,which affect yield and cause economic losses.To mitigate these impacts,farmers have started using biostimulants such as chitosan and plant growth promoting bacteria instead of agrochemicals due to their environmental and health benefits.This study evaluated the effect of Bacillus subtilis and chitosan,both individually and combined,on the growth,yield,and fruit quality of Soledad pepper under greenhouse conditions.Four treatments were applied at different stages of the crop cycle:Q(Chitosan),BS(Bacillus subtilis),Q+BS(Chitosan+Bacillus subtilis),and T(Control).The results showed that the combination of chitosan and Bacillus subtilis significantly improved plant growth,especially in height and stem diameter.The chitosan treatment produced the highest number of fruits and plant weight,while the combination of chitosan and Bacillus subtilis enhanced fruit quality,increasing characteristics such as size,weight,pericarp thickness,and physicochemical parameters,notably in brix degrees,citric acid percentage,and pH,outperforming the individual treatments and the control. 展开更多
关键词 Capsicum annuum L. chitosan Bacillus subtilis fruit quality
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产去鳞蛋白酶海洋Bacillus sp.TS1的筛选鉴定及去鱼鳞效果研究 认领 引用
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作者 田慧丽 周俊栏 +4 位作者 高源 张帅 章均哲 孙颖颖 王淑军 《保鲜与加工》 CAS 北大核心 2026年第3期98-106,共9页
为探究蛋白酶在促进鱼类脱鳞中的应用效果,利用其水解鱼皮与鱼鳞中胶原蛋白的能力,降低二者间的生物结合力,从而提升去鳞效率。采用透明圈法从海洋样品中筛选产蛋白酶的菌株,通过生物结合力测定和感官评定方法进一步筛选具去鳞效果的蛋... 为探究蛋白酶在促进鱼类脱鳞中的应用效果,利用其水解鱼皮与鱼鳞中胶原蛋白的能力,降低二者间的生物结合力,从而提升去鳞效率。采用透明圈法从海洋样品中筛选产蛋白酶的菌株,通过生物结合力测定和感官评定方法进一步筛选具去鳞效果的蛋白酶,并评估其脱鳞性能。结果表明,经过筛选得到菌株TS1蛋白酶去鳞效果较好,该菌鉴定为芽孢杆菌属(Bacillus sp.),其产酶最适温度为30℃,最适pH为8.0,最适碳源为麦芽糖,最适氮源为鱼粉蛋白胨,该菌在NaCl质量分数为1%时产酶最高。在加酶量为12 U/mL,15℃条件下对新鲜鲫鱼作用1 h,联合滚桶去鳞3 min,鲫鱼的去鳞率与对照组相比显著提高,达到(98.20±0.09)%,鱼鳞与鱼皮间的生物结合力从(1.24±0.38)N下降至(0.89±0.36)N。菌株TS1所产蛋白酶能够有效促进鱼鳞脱落,显著提高去鳞效率,具有良好的应用前景,可用于水产品精深加工领域。 展开更多
关键词 去鳞蛋白酶 Bacillus sp.TS1 海洋微生物 酶法去鳞
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Bacillus velezensis mitigates chronic LPS-induced lung injury of broilers via microbiota-driven isoflavone production and NF-κB/PPAR-γaxis modulation 认领 引用
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作者 Xunbozan Zhang Xuan Liu +5 位作者 Ye Xu Dadan Liu Ying Chen Christopher Peterson Daniel Ruixiao Mao Liang Chen 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第4期2195-2214,共20页
Background Chronic exposure to low-dose lipopolysaccharide(LPS)in poultry farming environments induces persistent respiratory inflammation,resulting in lung injury and impaired growth performance in broilers.Bacillus ... Background Chronic exposure to low-dose lipopolysaccharide(LPS)in poultry farming environments induces persistent respiratory inflammation,resulting in lung injury and impaired growth performance in broilers.Bacillus velezensis(BV)is a probiotic with known antibacterial and immunomodulatory activities,yet its role in respiratory health remains poorly understood.This study aimed to assess the potential benefits of BV in alleviating chronic pneumonia triggered by LPS in broilers and to clarify its mechanistic pathways.Results A chronic LPS intratracheal instillation model was established,comprising control,LPS,and BV+LPS groups.BV supplementation significantly ameliorated LPS-induced growth impairment(P<0.05),inhibited the synthesis of key inflammatory mediators,and mitigated oxidative stress in serum and bronchoalveolar lavage fluid(P<0.05).Integrated multi-omics analyses revealed that BV remodeled the pulmonary microbiota,enriching isoflavone-metabolizing taxa including Blautia and unclassified Lachnospiraceae(P<0.05),which was associated with elevated pulmonary concentrations of daidzein,genistein,and glycitein(P<0.05).Transcriptomic together with molecular analyses revealed that BV enhanced the activation of PPAR-γwhile attenuating NF-κB pathway activity,thereby reducing the expression of genes associated with inflammation(P<0.05).In vitro,experiments showed that daidzein and genistein inhibited cellular inflammatory responses through PPAR-γsignaling.BV culture supernatant directly suppressed NF-κB/NLRP3 inflammasome activation in chicken HD11 macrophages,reduced intracellular reactive oxygen species(ROS)generation,and shifted macrophage polarization toward an anti-inflammatory phenotype(P<0.05).Conclusion These findings demonstrate that BV alleviates LPS-induced chronic pneumonia through two complementary pathways,as it remodels the pulmonary microbiota to enhance isoflavone metabolism and thereby suppress inflammation,while its own metabolites also directly inhibit inflammatory signaling.This study provides new insight into probiotic-based interventions for respiratory health in livestock. 展开更多
关键词 Bacillus velezensis Broiler lung Chronic inflammation Isoflavone Lipopolysaccharide NF-κB
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In vitro selection,assessment,and identification of poten-tial probiotic features of the Bacillus strains isolated from the intestine of Nile tilapia(Oreochromis niloticus) 认领 引用
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作者 Vicent Michael Shija Kwaku Amoah +3 位作者 Yi Li Yong Zhong Xiaopiao Zhong Jia Cai 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2025年第7期116-131,共16页
In this study,we aimed to isolate potential Bacillus strains from the digestive tract of Nile tilapia(Oreochromis niloticus).Through morphological,biochemical,16S rDNA sequencing,and evolutionary relationship assessme... In this study,we aimed to isolate potential Bacillus strains from the digestive tract of Nile tilapia(Oreochromis niloticus).Through morphological,biochemical,16S rDNA sequencing,and evolutionary relationship assessments,we identified the isolated species as Bacillus amyloliquefaciens AV5(OR647358),Bacillus subtilis AV7(LC781790),and Bacillus velezensis AV50(OR647359),designated as AV5,AV7,and AV50 strains,respectively.We evaluated their probiotic potential,including tolerance to high bile salt concentrations,low pH levels,and high temperatures,as well as their adhesion abilities(auto-aggregation and cell-surface hydrophobicity),antimicrobial activity,biosafety,compatibility,hemolytic activity,and antibiotic susceptibility.AV5,AV7,and AV50 strains exhibitedγ-hemolytic activity and resistance to low pH(1)and high bile salt concentrations(0.5%).They demonstrated higher viability after exposure to elevated temperatures(80℃,90℃,and 100℃)and increased cell surface hydrophobicity and autoaggregation.These strains showed positive compatibility,indicating their potential for multispecies use.Strains were susceptible to 17 out of 20 tested antibiotics,and displayed significant antimicrobial activity against Vibrio alginolyticus,Vibrio harveyi,Streptococcus iniae,and Streptococcus agalactiae.Therefore,AV5,AV7,and AV50 are promising probiotic candidates for enhancing the growth and health of aquatic animals,particularly in Nile tilapia(O.niloticus). 展开更多
关键词 Oreochromis niloticus probiotics Bacillus amyloliquefaciens Bacillus velezensis Bacillus subtilis
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Corrigendum to“Transcriptomic and biochemical analysis of the mechanism of sodium gluconate promoting the degradation of benzo[a]pyrene by Bacillus subtilis MSC4”[Journal of Environmental Sciences,Volume 152,June 2025,Pages 39-53] 认领 引用
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《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第2期789-791,共3页
Rui Chena,b,Tangbing Cui a,b,∗a School of Biology and Biological Engineering,South China University of Technology,Guangzhou 510006,China b Guangdong Key Laboratory of Fermentation and Enzyme Engineering,South China Un... Rui Chena,b,Tangbing Cui a,b,∗a School of Biology and Biological Engineering,South China University of Technology,Guangzhou 510006,China b Guangdong Key Laboratory of Fermentation and Enzyme Engineering,South China University of Technology,Guangzhou 510006,China The authors regret that the published version of this article contained several errors and omissions,which are described and corrected below.1.Figs.3 and 4(figure order and legends).In the published article,Figs.3 and 4 were inadvertently published in reversed order.The figures should be swapped so that the figure content matches its caption.The correct figures and their legends are provided on the following page.2.Title correction.The compound name in the published title was incorrectly typeset as“benzo[a]pyrene”The correct spelling is“benzo[a]pyrene.”3.Text corrections in Section 2.4.Several typographical errors occurred in Section 2.4(“Up-regulation of acetoin,lactate,and kanosamine biosynthesis under sodium gluconate treatment”). 展开更多
关键词 benzo pyrene degradation correction transcriptomic analysis biochemical analysis corrigendum sodium gluconate Bacillus subtilis Msc enzyme engineeringsouth
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Solid-state fermentation of soybean residue by Bacillus subtilis for surfactin production and its application prospects 认领 引用
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作者 ZHAO Feng XU Xiaomeng +2 位作者 LIU Jifang CHEN Mengyang ZHENG Mengyao 《微生物学通报》 CAS CSCD 北大核心 2026年第3期1247-1262,共16页
[Background]Surfactin is a biosurfactant with remarkable surface/interfacial activity.Surfactin production suffers from high costs of carbon sources and severe foaming problem during fermentation.Unreasonable utilizat... [Background]Surfactin is a biosurfactant with remarkable surface/interfacial activity.Surfactin production suffers from high costs of carbon sources and severe foaming problem during fermentation.Unreasonable utilization of soybean residue(okara)can cause resource waste and environmental pollution.[Objective]To achieve sustainable production of surfactin and valueadded conversion of okara,we explored foam-free production of surfactin by Bacillus subtilis using okara as a low-cost substrate and evaluated its application prospects.[Methods]We evaluated and compared the feasibility of B.subtilis utilizing okara to synthesize surfactin through liquid and solid-state fermentation methods.Biosurfactants were extracted from solid-state culture via a weak alkaline water extraction method.The products were identified by HPLC-MS,and the physicochemical properties of the produced surfactin were analyzed.The solid-state medium for fermentation of okara was optimized by the response surface method.The viable count of B.subtilis in solid-state fermentation residue was determined by the plate colony counting method.[Results]The conversion rates of okara to surfactin were 0.6%−0.8%and 1.2%−1.5%in liquid and solid-state fermentation,respectively.Interestingly,solid-state fermentation of okara by B.subtilis achieved both high-yield and foam-free production of surfactin.Five surfactin homologues were produced from okara,mainly including surfactin-C13(34.16%),surfactin-C14(23.95%),and surfactin-C15(35.14%).The produced surfactin,with a critical micelle concentration of 35.0 mg/L,decreased water surface tension to(26.0±0.1)mN/m and emulsified crude oil with emulsifying activity index(EI24)(73.1±3.2)%.It was stable at 4−121℃,pH 5.0−11.0,and NaCl<150 g/L.Okara,NH4Cl,and CaCl2·2H2O were significant components in the solid-state medium.The surfactin yield was increased by 52.1%through solid-state medium optimization.Adding wheat straw further enhanced surfactin production by improving aeration in the solid-state medium.B.subtilis AnPL-1 produced(263.2±7.8)mg surfactin in the optimized solid-state medium containing 14.8 g okara and 1.5 g wheat straw.The conversion rate of okara to surfactin was enhanced to 1.8%.In addition,the residue of solid-state fermentation was expected to be microbial fertilizer since it contained 4.27×1010CFU/g of B.subtilis.[Conclusion]This study established a promising way for foam-free production of surfactin and value-added conversion of okara. 展开更多
关键词 biosurfactants okara solid-state fermentation Bacillus subtilis response surface method
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