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RNA interference affects tumorigenicity and expression of insulin-like growth factor-1,insulin-like growth factor-1 receptor,and basic fibroblast growth factor-2 in rat C6 glioma cells 认领 引用
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作者 Wanli Dong Jin Hu +3 位作者 Shaoyan Hu Yuanyuan Wang Juean Jiang Youxin Jin 《Neural Regeneration Research》 SCIE CAS 2009年第8期597-605,共9页
BACKGROUND： Human gliomas are more likely to express basic fibroblast growth factor-2 （FGF-2） insulin-like growth factor-1（IGF-1）, and IGF-1 receptor （IGF-1R） than normal brain tissue. These factors activate si... BACKGROUND： Human gliomas are more likely to express basic fibroblast growth factor-2 （FGF-2） insulin-like growth factor-1（IGF-1）, and IGF-1 receptor （IGF-1R） than normal brain tissue. These factors activate signal transduction systems of Ras/MAPK and PI3K/Akl, which promote glioma growth. OBJECTIVE： To utilize RNA interference （RNAi） technique to down-regulate FGF-2, IGF-1, and IGF-1R gene expression, and to investigate the effects of these genes on rat C6 glioma cells, as well as the feasibility of RNAi for treating glioma. DESIGN, TIME AND SETTING： This neurooncological, randomized, controlled, in vivo and in vitro experiment, which used RNAi methodology, was performed at the Laboratory of Molecular Biology, Institute of Biochemistry, Chinese Academy of Sciences between August 2005 and February 2008. MATERIALS： Rat C6 cell lines were purchased from Shanghai Institute of Cellular Biology Affiliated to Chinese Academy of Sciences. Small interfering RNA （siRNA） was synthesized by Shanghai GenePharma. Anti-IGF-1, anti-IGF-1R, anti-FGF-2, anti-mouse and anti-rabbit IgG G1-HRP antibodies were provided by Santa Cruz Biotechnology, USA. Four to six week-old BALB/c nude mice were purchased from the Laboratory Animal Center, Chinese Academy of Sciences. METHODS： C6 glioma cells were transfected with siRNA, which was chemically synthesized in vitro to correspond to endogenous FGF-2, IGF-1, and IGF-1R genes. The inhibition ratio of targeting mRNA expression was detected by semiquantitative RT-PCR, and protein expression was determined by Western blot analysis. C6 glioma cell proliferation was observed using a growth curve C6 glioma cell apoptosis rate and cell cycle were detected by flow cytometry. C6 glioma cell growth regression was observed by transwell migration assay. In addition, nude mouse subcutaneous tumor models were used in this study. For studying the anti-tumor effects of IGF-1 and IGF-1R siRNA, two blank control groups, with six mice each, were set up： A （2.5 μg siRNA was injected one week after C6 cells were inoculated, Le., when tumor volume reached 8 mm × 8 mm） and B （siRNA was injected at the same time with C6 cells were inoculated. To study the effects of FGF-2 siRNA, the groups consisted of a blank control group, negative control group, 2.6 μg siRNA group, 4 μg siRNA group, and 5.3 μg siRNA group, with six mice each. MAIN OUTCOME MEASURES： mRNA and protein inhibition ratio of FGF-2, IGF-1, and IGF-1 R; C6 glioma cell proliferation, apoptosis, and cycle growth arrest; C6 glioma cell growth regression and subcutaneous tumorigenicity rates. RESULTS： All siRNA constructs proved to be effective. After 48 hours, transfection of 200 nmol/L siRNA resulted in a FGF-2 or IGF-1R gene inhibition ratio 〉 80% and an IGF-1 gene inhibition ratio of approximately 70%. Protein expression levels for FGF-2, IGF-1, and IGF-1R decreased in a dose-dependent manner following siRNA transfection, with an inhibition rate 〉 85%, 60%, and 50%, respectively. C6 glioma cell proliferation and apoptosis rates increased in proportion to siRNA. The apoptosis rate of C6 glioma cells induced by FGF-2, IGF-1, and IGF-1R siRNA was 39.96%, 15.07% and 22.47%, respectively （P 〈 0.01）. Transfection of 200 nmol/L IGF or IGF-1R siRNA for 48 hours suppressed C6 glioma cell migration. At 30 days after intratumoral injection of 2.6, 4, and 5.3 tJg FGF-2 siRNA, tumor growth regression rate of FGF-2 siRNA was 56%, 67%, and 86%, respectively. The tumor growth regression rate was 71.88% and 45.71%, respectively, when IGF-1 or IGF-1R siRNA was intratumorally injected 1 week after C6 glioma cell transplantation. When IGF-1 or IGF-1 R siRNA was intratumorally injected during C6 glioma cell transplantation, the tumor growth regression rate was 78.13% and 74.29%, respectively. CONCLUSION： siRNA transfection downregulated gene expression of FGF-2, IGF-1, and IGF-1R In addition, siRNA treatment markedly suppressed glioma cell proliferation, growth, and migration, and concomitantly reduced subcutaneous tumorigenicity. 展开更多
关键词 small interference RNA basic fibroblast growth factor-2 insulin-like growth factor 1 insulin-like growth factor 1 receptor C6 glioma cell line
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Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells 认领 引用 被引量：3
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作者 Xin-Yi Fu Xiao-Feng Zhang +4 位作者 Wei Xia Lei Zhong Ying-Ming Wang Zheng-Tai Sun Jing Xia 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2015年第5期904-909,共6页
AIMTo expose rat retinal M&#x000fc;ller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor... AIMTo expose rat retinal M&#x000fc;ller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-&#x003b2;1 (TGF-&#x003b2;1) expression.METHODSThree groups of rat retinal M&#x000fc;ller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-&#x003b2;1 mRNA expression, protein levels and fluorescence intensity of the M&#x000fc;ller cells were analyzed.RESULTSThe bFGF mRNA expression and protein levels were significantly upregulated in M&#x000fc;ller cells in all three experimental groups compared with the control group (P&#x0003c;0.05), while that of TGF-&#x003b2;1 was downregulated (P&#x0003c;0.05). Also, bFGF expression was positively correlated, but TGF-&#x003b2;1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-&#x003b2;1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P&#x0003c;0.05).CONCLUSIONThe expressions of bFGF and TGF-&#x003b2;1 changed in a time-dependent manner in M&#x000fc;ller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. M&#x000fc;ller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-&#x003b2;1 expression. Changes in cytokine expression in retinal M&#x000fc;ller cells may affect monochromatic light-induced myopia. 展开更多
关键词 monochromatic light myopia Mü ller cells basic fibroblast growth factor transforming growth factor-β 1
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Efficacy of Ciji Hua'ai Baosheng formula on the expressions of vascular endothelial growth factor,kinase insert domain-containing receptor and basic fibroblast growth factor in mouse models of H22 hepatocellular carcinoma 认领 引用 被引量：1
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作者 Zhao Xinyue Xi Shengyan +6 位作者 Wang Yanhui Xu Yangxinzi Pollock Galia Su Yu Cheng Yao Loy Guanjie Liu Pei 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2017年第1期88-95,共8页
OBJECTIVE: To investigate the efficacy of Ciji Hua'ai Baosheng formula(CHBF) on microvessel density(MVD) and vascular endothelial growth factor(VEGF), kinase insert domain-containing receptor(KDR) and basic fibrob... OBJECTIVE: To investigate the efficacy of Ciji Hua'ai Baosheng formula(CHBF) on microvessel density(MVD) and vascular endothelial growth factor(VEGF), kinase insert domain-containing receptor(KDR) and basic fibroblast growth factor(b FGF) expression in serum and tumor tissue of mice receiving chemotherapy for the treatment of H22 hepatocellular carcinoma.METHODS: Sixty Kunming mice were injected subcutaneously with H22 hepatoma carcinoma cell suspensions into the right anterior armpit. Seven days later, all transplanted tumor were formed and the mice were intraperitoneally injected 200 mg/kg cytoxan(CTX) to establish the models of tumor-bearing mouse chemotherapy, then they were randomly divided into model group, continuing CTX chemotherapy group(CTX group), and three CHBF(117, 58.5 and 29.25 g/kg) groups. After ten days of treatments, histology was observed, contents of VEGF, KDR and b FGF in serum and tumor tissue were measured by enzyme-linked immunosorbent assay(ELISA), VEGF and b FGF protein expression and MVD tagged by CD34 were detected by immunohistochemisty.RESULTS: MVD in CHBF(117, 58.5 g/kg) and CTX groups was significantly lower than that in model group(P < 0.01); expressions of VEGF, KDR and b FGF in serum and tumor tissue in CHBF(117 g/kg)group were less than those in model group(P <0.05; P < 0.01); the expressions of MVD, VEGF and b FGF in tumor tissue of CHBF(117 g/kg) groupwere also less than those in CTX group(P < 0.05;P < 0.01).CONCLUSION: CHBF can effectively reduce the expression of VEGF, KDR and b FGF in serum and tumor tissue, and decrease MVD and delay tumor progression. 展开更多
关键词 Drug therapy Angiogenesis inhibitors Vascular endothelial growth factors Receptors vascular endothelial growth factor Basic fibroblast growth factor 2 Ciji Hua’ai Baosheng formula
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Expression of fibroblast growth factor-2 and fibroblast growth factor receptor-1 protein in the hippocampus in rats exhibiting chronic stress-induced depression 认领 引用
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作者 Gonglin Hou Mingming Tang 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第13期1010-1016,共7页
There is evidence that the expression of members of the fibroblast growth factor(FGF)protein family is altered in post-mortem brains of humans suffering from major depressive disorder.The present study examined whethe... There is evidence that the expression of members of the fibroblast growth factor(FGF)protein family is altered in post-mortem brains of humans suffering from major depressive disorder.The present study examined whether the expression of fibroblast growth factor-2(FGF2)and fibroblast growth factor receptor-1(FGFR1)protein is altered following chronic stress in an animal model.Rats were exposed to 35 days of chronic unpredictable mild stress,and then tested using open-field and sucrose consumption tests.Compared with the control group,rats in the chronic stress group exhibited obvious depressive-like behaviors,including anhedonia,anxiety and decreased mobility.The results of western blot analysis and immunohistochemical analysis revealed a downregulation of the expression of FGF2 and FGFR1 in the hippocampus of rats,particularly in the CA1,CA3 and dentate gyrus.This decreased expression is in accord with the results of post-mortem studies in humans with major depressive disorder.These findings suggest that FGF2 and FGFR1 proteins participate in the pathophysiology of depressive-like behavior,and may play an important role in the mechanism of chronic stress-induced depression. 展开更多
关键词 depression hippocampus fibroblast growth factor-2 fibroblast growth factor receptor-1 neural regeneration
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Effect of basic fibroblast growth factor and danshen on bcl-2 and p53 mRNA expression in the brain of rats exposed to repeated,high,positive acceleration(+Gz) 认领 引用
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作者 Hongjin Liu Qing Cai 《Neural Regeneration Research》 SCIE CAS 2008年第7期747-750,共4页
BACKGROUND:Both animal experiments and clinical studies have shown that basic fibroblast growth factor(bFGF)and danshen(Salvia miltiorrhiza)can exhibit protective effects on ischemia-reperfusion cerebral injury.OBJECT... BACKGROUND:Both animal experiments and clinical studies have shown that basic fibroblast growth factor(bFGF)and danshen(Salvia miltiorrhiza)can exhibit protective effects on ischemia-reperfusion cerebral injury.OBJECTIVE:To test whether bFGF and danshen can protect cerebral injury induced by exposure to repeated,high,positive acceleration(+Gz)in an animal model and to analyze the possible mechanisms.DESIGN,TIME AND SETTING:Randomized controlled animal study.The experiment was performed at the Research Center for Molecular Biology,Air-force General Hospital of Chinese PLA from April to August 2000.MATERIALS:A total of 20 clean grade,healthy,Sprague Dawley rats of both genders,weighing(200±15)g,were provided by our experimental animal center.Rats were randomly divided into 5 groups:the control group,+Gz exposure group,bFGF group,danshen group,and saline group,with 4 animals per group.bFGF(Beijing Bailuyuan Biotechnology Co.Ltd.)and danshen solution(Shanghai Zhongxi Pharmaceutical Co.Ltd.)were used.METHODS:All rats were fixed on a rotary arm of a centrifugal apparatus(2 m in radius)with their heads oriented towards the center of the apparatus.Except for rats in the control group,the value of+Gz exposure was+14 Gz with an acceleration rate of 1.5 G/s.The peak force lasted for 45 seconds.+Gz exposure was performed three times with intervals of 30 minutes.Rats in the control group received the same+Gz procedure,but the G value was+1 Gz.Rats in bFGF group and danshen group were intraperitoneally injected with 100μg/kg bFGF or 15 g/kg danshen solution,respectively,at 30 minutes prior to centrifugation and immediately after centrifugation.Rats in saline group were injected with the same volume of saline.Six hours after exposure,rats were decapitated.One hemisphere was preserved in liquid nitrogen for RNA extraction and the other was processed for apoptosis detection.MAIN OUTCOME MEASURES:mRNA levels of bcl-2 and p53 were measured by semi-quantitative reverse-transcription polymerase chain reaction.Apoptotic cell death was detected by terminal deoxynuleotidyl transferase-mediated dUTP nick end labeling.RESULTS:Changes in mRNA expression of bcl-2 and p53 and apoptotic cells were observed in rat brain six hours after repeated+Gz exposures,bFGF and danshen were able block the changes of bcl-2 and p53 expression and inhibit apoptotic cell death.CONCLUSION:The data suggest that apoptosis and changes in bcl-2 and p53 expression in the rat brain can be induced by repeated+Gz exposures.Apoptosis is,therefore,one of the molecular mechanisms of brain damage induced by repeated+Gz exposures,bFGF and danshen were of the equal potency in preventing brain injury induced by repeated+Gz exposures. 展开更多
关键词 positive acceleration rats apoptosis bcl-2 p53 gene expression basic fibroblast growth factor danshen
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Effect of charge at an amino acid of basic fibroblast growth factor on its mitogenic activity 认领 引用 被引量：1
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作者 Wu, Xiao Ping Li, Xiao Kun 《Chinese Chemical Letters》 SCIE CAS 2010年第4期468-471,共4页
The amino acid at the 119th position of human basic fibroblast growth factor(hbFGF),lysine(K119),is a critical component for its mitogenic activity.However,little is known about the effects of the characteristics of t... The amino acid at the 119th position of human basic fibroblast growth factor(hbFGF),lysine(K119),is a critical component for its mitogenic activity.However,little is known about the effects of the characteristics of this residue including charge on the mitogenic activity of hbFGF.Herein,this basic residue was replaced with neutral glutamine residue and acidic glutamic acid residue to construct mutants hbFGFK119Q and hbFGFK119E,respectively.The mutants were produced by BL21(DE3)/pET3c expression system... 展开更多
关键词 Human basic fibroblast growth factor Amino acid charge Expression Mitogenic activity Erk1/2 activation
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健脾补肾益肺方联合布地格福治疗肺脾气虚型慢性阻塞性肺疾病稳定期患者疗效及对血清bFGF、COX-2水平的影响 认领 引用
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作者 吴峥嵘 李渊 郝素英 《广州中医药大学学报》 CAS 2026年第1期63-71,共9页
【目的】探究健脾补肾益肺方联合布地格福治疗肺脾气虚型慢性阻塞性肺疾病稳定期患者的疗效及其对血清碱性成纤维细胞生长因子(bFGF)、环加氧酶2(COX-2)的影响。【方法】选取2022年2月至2024年1月北京中医药大学东方医院收治的肺脾气虚... 【目的】探究健脾补肾益肺方联合布地格福治疗肺脾气虚型慢性阻塞性肺疾病稳定期患者的疗效及其对血清碱性成纤维细胞生长因子(bFGF)、环加氧酶2(COX-2)的影响。【方法】选取2022年2月至2024年1月北京中医药大学东方医院收治的肺脾气虚型慢性阻塞性肺疾病稳定期患者138例,采用随机数字表法将患者随机分为对照组和试验组,每组各69例。2组患者均给予加强营养、康复锻炼及避免有害因素等常规治疗,在此基础上,对照组给予布地格福吸入气雾剂吸入治疗,试验组在对照组的基础上联合健脾补肾益肺方治疗,疗程为3个月。观察2组患者治疗前后肺功能、血气指标、圣乔治呼吸问卷(SGRQ)评分、6 min步行距离(6MWD)及血清bFGF、COX-2水平的变化情况,比较2组患者的临床疗效,分析血清bFGF与COX-2水平、动脉血二氧化碳分压(PaCO2)、动脉血氧分压(PaO2)的相关性,并采用多因素Logistic回归分析肺脾气虚型慢性阻塞性肺疾病的影响因素。【结果】(1)疗效方面,治疗3个月后,试验组的总有效率为94.20%(65/69),对照组为78.26%(54/69),组间比较,试验组的疗效明显优于对照组(χ2=6.103,P<0.05)。(2)血清bFGF、COX-2水平方面,治疗后,2组患者的血清bFGF、COX-2水平均较治疗前降低(P<0.05),且试验组的降低幅度均明显优于对照组(P<0.01)。(3)肺功能方面,治疗后,2组患者的呼气峰流速(PEF)、第一秒用力呼气容积(FEV1)、第一秒用力呼气容积与用力肺活量的比值(FEV1/FVC)均较治疗前升高(P<0.05),且试验组的升高幅度均明显优于对照组(P<0.01)。(4)血气分析指标方面,治疗后,2组患者的PaCO2均较治疗前降低(P<0.05),PaO2均较治疗前升高(P<0.05),且试验组对PaCO2的降低幅度及对PaO2的升高幅度均明显优于对照组(P<0.01)。(5)生活质量方面,治疗后,2组患者的SGRQ评分均较治疗前降低(P<0.05),6MWD均较治疗前升高(P<0.05),且试验组对SGRQ评分的降低幅度及对6MWD的升高幅度均明显优于对照组(P<0.01)。(6)相关性分析方面,经对年龄、病程、烟酒史、糖尿病史、性别等校正后,血清bFGF与COX-2、PaCO2呈正相关(P<0.01),与PaO2呈负相关(P<0.01)。(7)肺脾气虚型慢性阻塞性肺疾病的影响因素方面,多因素Logistic回归结果显示:bFGF、COX-2、PaCO2、SGRQ评分为保护因素(P<0.01),PEF、FEV1、FEV1/FVC、PaO2、6MWD为危险因素(P<0.01)。【结论】健脾补肾益肺方联合布地格福治疗肺脾气虚型慢性阻塞性肺疾病稳定期患者疗效确切,能更有效地改善患者肺功能和血气指标,提高患者生活质量,降低血清bFGF、COX-2水平。 展开更多
关键词 健脾补肾益肺方 布地格福 肺脾气虚型 慢性阻塞性肺疾病稳定期 碱性成纤维细胞生长因子 环加氧酶2 肺功能 血气指标 生活质量
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垂体腺瘤组织中RAB1A、bFGF、SOX2的表达及与术后复发的关系 认领 引用
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作者 王振 史桃圳 +2 位作者 肖维汉 张桐 纵振坤 《分子诊断与治疗杂志》 2026年第1期9-11,15,共3页
目的探究垂体腺瘤组织中Ras相关蛋白Rab-1A(RAB1A)、碱性成纤维细胞生长因子(bFGF)、性别决定区Y框蛋白2(SOX2)的表达及与术后复发的关系。方法选取2022年1月至2025年1月徐州医科大学附属医院收治的100例垂体腺瘤患者临床资料,根据末次... 目的探究垂体腺瘤组织中Ras相关蛋白Rab-1A(RAB1A)、碱性成纤维细胞生长因子(bFGF)、性别决定区Y框蛋白2(SOX2)的表达及与术后复发的关系。方法选取2022年1月至2025年1月徐州医科大学附属医院收治的100例垂体腺瘤患者临床资料,根据末次随访患者预后情况将其分为复发组与未复发组,比较两组的RAB1A、bFGF、SOX2表达情况。比较两组患者临床资料,采用多因素Logistic回归分析探究垂体腺瘤患者术后复发的影响因素。结果100例患者中RAB1A、bFGF、SOX2高表达分别为67例(67.00%)、84例(84.00%)、82例(82.00%);末次随访显示,100例患者中共31例复发(复发组),复发率为31.00%,另69例未复发(未复发组);复发组RAB1A、SOX2高表达率以及bFGF阳性率高于未复发组,差异有统计学意义(P0.05);多因素Logistic回归分析结果显示,RAB1A、bFGF、SOX2为垂体腺瘤患者术后复发的影响因素(P<0.05)。结论临床可对垂体腺瘤患者进行RAB1A、bFGF、SOX2检测,对RAB1A、SOX2高表达及bFGF阳性患者需引起重视,尽早采取措施进行干预以降低患者术后复发率。 展开更多
关键词 垂体腺瘤 Ras相关蛋白Rab-1A 碱性成纤维细胞生长因子 性别决定区Y框蛋白2
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点阵CO2激光联合短波治疗仪导入rb-bFGF修复痤疮凹陷性瘢痕的临床观察 认领 引用 被引量：1
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作者 任晓萍 杨蓉娅 +3 位作者 解佳璐 庞亚妮 周丽媛 张纯 《中国美容医学》 CAS 2025年第10期108-112,共5页
目的:探讨点阵CO2激光联合短波治疗仪导入重组牛碱性成纤维细胞生长因子(Recombinant bovine basic fibroblast growth factor,rb-bFGF)凝胶修复痤疮凹陷性瘢痕的美学效果及安全性。方法:纳入2023年1月-2024年1月就诊于笔者医院的13... 目的:探讨点阵CO2激光联合短波治疗仪导入重组牛碱性成纤维细胞生长因子(Recombinant bovine basic fibroblast growth factor,rb-bFGF)凝胶修复痤疮凹陷性瘢痕的美学效果及安全性。方法:纳入2023年1月-2024年1月就诊于笔者医院的134例痤疮凹陷性瘢痕患者,根据随机数字表法将其分为对照组和观察组,各67例;对照组接受点阵CO2激光治疗,观察组接受点阵CO2激光联合短波治疗仪导入rb-bFGF凝胶治疗。比较两组临床疗效、皮肤生理指标、美学效果评分及不良事件。结果:观察组临床疗效率(94.03%)显著高于对照组(76.12%)(P0.05)。结论:点阵CO2激光联合短波治疗仪导入rb-bFGF用于痤疮凹陷性瘢痕患者的临床疗效确切,可改善皮肤生理指标,显著提升美学效果,且安全性良好。 展开更多
关键词 点阵CO2激光 修复 重组牛碱性成纤维细胞生长因子(rb-bFGF) 痤疮凹陷性瘢痕 美学 安全性 短波治疗
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血清ENA-78、bFGF和组织TGF-β1、COX-2表达水平与腹腔镜术后患者腹腔粘连的关系 认领 引用
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作者 赵朋伟 李干 李徐奇 《分子诊断与治疗杂志》 2025年第11期2060-2063,共4页
目的 探讨血清中性粒细胞活化肽-78(ENA-78)、碱性成纤维细胞生长因子(bFGF)和组织转化生长因子-β1(TGF-β1)、环氧化酶-2(COX-2)表达水平与腹腔镜术后患者腹腔粘连的关系。方法 按照1∶2比例分别纳入2023年1月至2024年12月西安交通大... 目的 探讨血清中性粒细胞活化肽-78(ENA-78)、碱性成纤维细胞生长因子(bFGF)和组织转化生长因子-β1(TGF-β1)、环氧化酶-2(COX-2)表达水平与腹腔镜术后患者腹腔粘连的关系。方法 按照1∶2比例分别纳入2023年1月至2024年12月西安交通大学第一附属医院收治的104例腹腔镜术后腹腔粘连患者和208例腹腔镜术后无腹腔粘连患者作为研究对象,并分别作为研究组和对照组。比较两组临床资料、血清ENA-78、bFGF和组织TGF-β1、COX-2表达水平,比较不同病理特征腹腔镜术后腹腔粘连患者血清ENA-78、bFGF和组织TGF-β1、COX-2表达水平,采用Spearman相关性分析腹腔镜术后腹腔粘连患者粘连程度与血清ENA-78、bFGF和组织TGF-β1、COX-2表达水平的相关性。结果 较对照组,研究组的血清ENA-78、bFGF水平及组织TGF-β1、COX-2高表达的占比均更高,差异有统计学意义(P<0.05)。较Ⅰ级/Ⅱ级,Ⅲ级/Ⅳ级的腹腔镜术后腹腔粘连患者血清ENA-78、bFGF水平及组织TGF-β1、COX-2高表达的占比均更高,差异有统计学意义(P<0.05)。腹腔镜术后腹腔粘连患者血清ENA-78、bFGF和组织TGF-β1、COX-2表达水平与粘连程度呈正相关(r=0.748、0.659、0.862、0.844,P<0.05)。结论 血清ENA-78、bFGF和组织TGF-β1、COX-2或可作为反映腹腔镜术后腹腔粘连患者粘连程度的潜在生物标志物。 展开更多
关键词 腹腔镜手术 腹腔粘连 中性粒细胞活化肽-78 碱性成纤维细胞生长因子 组织转化生长因子-β1 环氧化酶-2
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痤疮瘢痕CO2点阵激光术后应用bFGF临床疗效的Meta分析 认领 引用 被引量：2
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作者 陈蹬蹬 郭杏 +1 位作者 左彧之 孙一 《中国烧伤创疡杂志》 2025年第3期247-252,共6页
目的系统评价痤疮瘢痕CO2点阵激光术后应用碱性成纤维细胞生长因子(bFGF)治疗局部创面的临床疗效。方法检索Cochrane Library、中国学术期刊数据库(万方)等数据库建库至2023年8月公开发表的痤疮瘢痕CO2点阵激光术后应用bFGF治疗... 目的系统评价痤疮瘢痕CO2点阵激光术后应用碱性成纤维细胞生长因子(bFGF)治疗局部创面的临床疗效。方法检索Cochrane Library、中国学术期刊数据库(万方)等数据库建库至2023年8月公开发表的痤疮瘢痕CO2点阵激光术后应用bFGF治疗局部创面的中英文文献,经筛选后对符合要求的文献进行质量评价与数据分析。结果共纳入文献15篇,包含1266例痤疮瘢痕患者。Meta分析结果显示,与单纯应用CO2点阵激光照射相比,联合应用bFGF治疗的患者总有效率明显更高(OR=4.15,95%CI为2.63~6.56,P<0.00001)、红斑及结痂持续时间更短(SMD=-1.53、-1.85,95%CI为-2.38~-0.68、-2.67~-1.03,P=0.0004、P<0.00001)、痤疮瘢痕权重(ECCA)评分及不良反应发生率更低(SMD/OR=-1.96、0.31,95%CI为-2.85~-1.07、0.20~0.50,P均<0.00001)。结论与单纯应用CO2点阵激光相比,在其基础上联合应用bFGF治疗痤疮瘢痕,更能有效改善瘢痕症状,缩短激光术后红斑及结痂持续时间,降低不良反应发生率,疗效更显著。 展开更多
关键词 碱性成纤维细胞生长因子 CO2点阵激光 痤疮 瘢痕 Meta分析
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牛碱性成纤维细胞生长因子凝胶联合超脉冲点阵CO2激光治疗中重度痤疮的效果及对炎症反应、皮肤屏障功能的影响 认领 引用 被引量：2
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作者 杨彬彬 李秀荣 《临床医学研究与实践》 2025年第31期114-117,共4页
目的研究牛碱性成纤维细胞生长因子凝胶联合超脉冲点阵CO2激光治疗中重度痤疮的效果及对炎症反应、皮肤屏障功能的影响。方法将86例中重度痤疮患者以挂号单尾号单双数分为对照组(n=43,超脉冲点阵CO2激光治疗)和观察组(n=43,在对... 目的研究牛碱性成纤维细胞生长因子凝胶联合超脉冲点阵CO2激光治疗中重度痤疮的效果及对炎症反应、皮肤屏障功能的影响。方法将86例中重度痤疮患者以挂号单尾号单双数分为对照组(n=43,超脉冲点阵CO2激光治疗)和观察组(n=43,在对照组基础上给予牛碱性成纤维细胞生长因子凝胶治疗)。两组均给予口服盐酸多西环素片、外用夫西地酸乳膏和阿达帕林凝胶基本治疗。比较两组的治疗效果。结果观察组的治疗总有效率高于对照组(P<0.05)。观察组的皮损结痂时间、结痂脱落时间、愈合时间及红斑持续时间短于对照组(P<0.05)。治疗后,两组的C反应蛋白(CRP)、白细胞介素-6(IL-6)及降钙素原(PCT)水平均降低,且观察组低于对照组(P<0.05)。治疗后,观察组的皮肤红斑指数(EI)、pH及经皮水分丢失量(TEWL)低于对照组,角质层含水量高于对照组(P<0.05)。观察组的美观满意度高于对照组(P<0.05)。结论牛碱性成纤维细胞生长因子凝胶联合超脉冲点阵CO2激光治疗中重度痤疮的效果显著,可促进皮损愈合,改善炎症反应,调节皮肤屏障功能,且患者的满意度高。 展开更多
关键词 牛碱性成纤维细胞生长因子凝胶 超脉冲点阵CO2激光 中重度痤疮
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Material surface conjugated with fibroblast growth factor-2 for pluripotent stem cell culture and differentiation 认领 引用
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作者 Tzu-Cheng Sung Zhi-Xian Pan +11 位作者 Ting Wang Hui-Yu Lin Chia-Lun Chang Ling-Chun Hung Suresh Kumar Subbiah Remya Rajan Renuka Shih-Jie Chou Shih-Hwa Chiou Idaszek Joanna Henry Hsin-Chung Lee Gwo-Jang Wu Akon Higuchi 《Regenerative Biomaterials》 SCIE EI CSCD 2025年第4期84-100,共17页
Fibroblast growth factor-2(FGF-2)is a critical molecule for sustaining the pluripotency of human pluripotent stem(PS)cells.However,FGF-2 is extremely unstable and cannot be stored long periods at room temperature.Ther... Fibroblast growth factor-2(FGF-2)is a critical molecule for sustaining the pluripotency of human pluripotent stem(PS)cells.However,FGF-2 is extremely unstable and cannot be stored long periods at room temperature.Therefore,the following FGF-2-conjugated cell culture materials were developed to stabilize FGF-2:FGF-2-conjugated polyvinyl alcohol(PVAI-C-FGF)hydro-gels and FGF-2-conjugated carboxymethyl cellulose-coated(CMC-C-FGF)dishes.Human induced pluripotent stem(iPS)cells were proliferated on recombinant vitronectin(rVN)-coated PVAI-C-FGF hydrogels and CMC-C-FGF dishes in medium without FGF-2.Human iPS cells could not be cultivated on rVN-coated PVAI-C-FGF hydrogels for more than two passages but could proliferate on rVN-coated CMC-C-FGF dishes.These results indicated that the amount of immobilized FGF-2 and the base cell materials are important,including the amount of immobilized rVN and the conformation of FGF-2 on the surfaces.When human iPS cells were pro-liferated on rVN-coated CMC-C-FGF surfaces in medium containing no FGF-2 for 10 passages,their pluripotency and potential to differen-tiate into cells originating from three germ layers were maintained in vivo and in vitro.Furthermore,the cells could extensively differentiate into cardiomyocytes,which can be used for cardiac infarction treatment in future and retinal pigment epithe-lium for retinal pigmentosa treatment in future.The FGF-2-immobilized surface could enable human PS cell culture in medium that does not need to contain unstable FGF-2.The amount of FGF-2 immobilization on the rVN-coated CMC-C-5FGF and CMC-C-20FGF dishes was reduced to 93.6 and 52.2 times,respectively,which is less than the conventional amount of FGF-2 used in culture medium for one passage(6 days)of human iPS cell culture.This reduction resulted from the stabilization of unstable FGF-2 by the immobilization of FGF-2,which was achieved by utilizing optimal base materials(CMC),coating materials(rVN)and long-joint segment(PEG4-SPDP)design. 展开更多
关键词 human pluripotent stem cells carboxymethyl cellulose fibroblast growth factor-2 cell differentiation material design
Thermostabilizing Functional Proteins with Matrix-Assisted Room-Temperature Drying 认领 引用
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作者 Yejiong Yu Siqi Dai +2 位作者 Johnny Xiangyi Zhou Wei E.Huang Zhanfeng Cui 《Engineering》 SCIE EI CSCD 2026年第3期157-168,共12页
This study evaluated the efficacy of the proposed matrix-assisted room-temperature(MART)drying as an alternative to freeze-drying for the thermostabilization of functional proteins in a solid state.To achieve this,pro... This study evaluated the efficacy of the proposed matrix-assisted room-temperature(MART)drying as an alternative to freeze-drying for the thermostabilization of functional proteins in a solid state.To achieve this,protective agents were formulated with functional proteins,and the mixture was dried on a biocompatible cellulose fiber matrix.Drying was carried out at room temperature or elevated temperatures(~30℃),either through dry air circulation(MART-DA drying)or under a vacuum(MART-V drying).The entire drying process did not involve any refrigeration or freezing steps.The results demonstrated the successful thermostabilization of lactate dehydrogenase(LDH),fibroblast growth factor-2(FGF-2),and functional enzymes in reverse transcription loop-mediated isothermal amplification(RT-LAMP)reagents through the use of MART drying.The functional proteins were immobilized and effectively encapsulated in sugar glass films,preserving the proteins'native structure and functions.The sugar glass films were supported by a low-cost three-dimensional(3D)cellulose fiber matrix.Overall,MART drying offers a simple,fast,low energy-consumption,and cost-effective strategy for drying functional proteins for long-term room-temperature storage. 展开更多
关键词 Protein stabilization Matrix-assisted room temperature drying Bioproduct drying Lactate dehydrogenase Fibroblast growth factor-2 RT-LAMP reagents
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骨形态发生蛋白-2与碱性成纤维细胞生长因子在异位和原位成骨中的作用 认领 引用 被引量：13
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作者 王磊 章燕 +2 位作者 游素兰 谭鸾君 黄远亮 《华西口腔医学杂志》 CAS CSCD 北大核心 2012年第4期420-424,共5页
目的通过骨髓基质细胞(BMSCs)的体外增殖和分化、异位成骨和原位成骨实验来观察骨形态发生蛋白-2(BMP-2)和碱性成纤维细胞生长因子(bFGF)在成骨过程中的作用。方法分别用含BMP-2、bFGF和BMP-2+bFGF的培养液体外培养Beagle犬的BMSCs,通... 目的通过骨髓基质细胞(BMSCs)的体外增殖和分化、异位成骨和原位成骨实验来观察骨形态发生蛋白-2(BMP-2)和碱性成纤维细胞生长因子(bFGF)在成骨过程中的作用。方法分别用含BMP-2、bFGF和BMP-2+bFGF的培养液体外培养Beagle犬的BMSCs,通过甲基噻唑基四唑(MTT)比色法测定细胞增殖水平,通过测定碱性磷酸酶(ALP)活性观察细胞的分化情况。将BMSCs与多孔磷酸钙(CPC)分别在含BMP-2、bFGF和BMP-2+bFGF的培养液中复合培养,制成复合材料,一部分植入裸鼠皮下,观察异位成骨情况,另一部分植入Beagle犬的种植体周围骨缺损区,经过荧光标记观察原位成骨情况。结果含有BMP-2+bFGF的培养液促进BMSCs增殖和分化的能力最强。异位成骨情况:BMP-2+bFGF组的成骨量较其他组明显增加,其新骨形成百分比为48.79%±11.31%,高于单一BMP-2组(30.71%±10.85%)和bFGF组(27.33%±9.67%)以及对照组(10.65%±6.05%)。原位成骨术后12周,BMP-2+bFGF组的矿化沉积率高于其他组,其差异有统计学意义(P<0.01)。结论在促进成骨方面,BMP-2和bFGF共同作用优于单一因子。 展开更多
关键词 骨髓基质细胞 骨形态发生蛋白-2 碱性成纤维细胞生长因子 成骨
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环氧合酶-2和碱性成纤维细胞生长因子在鼻咽癌组织中的表达及与放疗敏感性的相关性 认领 引用 被引量：5
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作者 赵建夫 徐萌 +3 位作者 陈文慧 赵凤芝 王奕鸣 庄承海 《南方医科大学学报》 CAS CSCD 北大核心 2016年第12期1718-1722,共5页
目的探讨环氧合酶-2(COX-2)和碱性成纤维细胞生长因子(bFGF)在鼻咽癌组织中的表达及其与放疗敏感性的相关性。方法应用免疫组化方法检测97例鼻咽癌患者活检组织放疗前COX-2和bFGF的表达,并分析二者与放疗敏感性的关系。结果97例鼻咽癌组... 目的探讨环氧合酶-2(COX-2)和碱性成纤维细胞生长因子(bFGF)在鼻咽癌组织中的表达及其与放疗敏感性的相关性。方法应用免疫组化方法检测97例鼻咽癌患者活检组织放疗前COX-2和bFGF的表达,并分析二者与放疗敏感性的关系。结果97例鼻咽癌组织COX-2和bFGF阳性表达率分别为71.1%(69/97)和64.9%(63/97);COX-2的阳性表达与鼻咽癌的T分期和N分期有显著的相关性(P<0.05),bFGF的阳性表达与鼻咽癌的N分期有显著的相关性(P<0.05)。COX-2在放疗敏感组和放疗不敏感组的阳性表达率分别为62.8%和92.6%,bFGF在放疗敏感组和放疗不敏感组的阳性表达率分别为57.1%和85.2%,在鼻咽癌组织中Spearman相关分析发现,COX-2的表达与bFGF的表达呈明显正相关(相关系数r=0.486,P<0.05);根据COX-2和bFGF表达情况,把患者分为4组:COX-2(-)and bFGF(-),COX-2(-)and bFGF(+),COX-2(+)and bFGF(-),and COX-2(+)and bFGF(+)。联合COX-2表达和bFGF表达分析后,发现不同亚组与鼻咽癌的放疗敏感性明显相关(P<0.05)。结论 COX-2和bFGF在鼻咽癌组织中的表达升高,且两者与肿瘤的放疗敏感性明显相关,可作为预测鼻咽癌放疗敏感性的重要指标。 展开更多
关键词 鼻咽癌 环氧合酶-2 碱性成纤维细胞生长因子 放疗敏感性
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Roles of cyclooxygenase-2 in microvascular endothelial cell proliferation induced by basic fibroblast growth factor 认领 引用 被引量：5
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作者 QIAN Rui-zhe YUE Fei +3 位作者 ZHANG Guo-ping HOU Li-kun WANG Xin-hong JIN Hui-ming 《Chinese Medical Journal》 SCIE CAS 2008年第24期2599-2603,共5页
Background The level of basic fibroblast growth factor(bFGF)increases rapidly after cerebral ischemia.However,the molecular mechanisms for the effects of bFGF on cerebral microvascular endothelial cells(cMVECs)have no... Background The level of basic fibroblast growth factor(bFGF)increases rapidly after cerebral ischemia.However,the molecular mechanisms for the effects of bFGF on cerebral microvascular endothelial cells(cMVECs)have not yet been fully elucidated.In this study,a murine cMVEC line,bEnd.3,was employed to study the effects of bFGF on cyclooxygenase(COX)expression and its downstream effects in cMVECs.Methods After treatment with bFGF,RT-PCR and Western blotting analyses were carried out to evaluate the changes in COX-2 mRNA and protein expression,respectively.MTT assays were performed to measure cell proliferation.The prostaglandin E2(PGE2)and vascular endothelial growth factor(VEGF)concentrations in the culture medium were measured by enzyme-linked immunosorbent assay(ELISA).Results COX-2 mRNA and protein expressions in bEnd.3 cells were induced by bFGF in time-and dose-dependent manners.The bFGF-induced COX-2 upregulation led to enhanced PGE2 production by bEnd.3 cells,and this effect was abolished by the selective COX-2 inhibitor NS-398.bFGF also increased VEGF production by bEnd.3 cells,and this effect was blocked by NS-398 and the EP1/2(PGE2 receptors)antagonist AH6809.Furthermore,exogenous PGE2 increased VEGF production in bend.3 cells,and AH6809 blocked this effect.Conclusion bFGF increases VEGF production in an autocrine manner by increasing COX-2-generated PGE2 in cMVECs and subsequently stimulates MVEC proliferation and angiogenesis. 展开更多
关键词 cerebral ischemia cerebral microvascular endothelial cell basic fibroblast growth factor cyclooxygenase prostaglandin E2 vascular endothelial growth factor
COX-2、bFGF及TGFβ1在慢性粒细胞白血病中的表达及其意义 认领 引用 被引量：9
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作者 王晓军 茹甫毅 +2 位作者 吴双有 朱卫民 田培军 《现代肿瘤医学》 CAS 2017年第4期603-607,共5页
目的:检测不同分期慢性粒细胞白血病(chronic myeloid leukemia,CML)患者血清环氧合酶-2(serum cyclooxygenase-2,COX-2)、碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)及转化生长因子(transforming growth factorβ1... 目的:检测不同分期慢性粒细胞白血病(chronic myeloid leukemia,CML)患者血清环氧合酶-2(serum cyclooxygenase-2,COX-2)、碱性成纤维细胞生长因子(basic fibroblast growth factor,b FGF)及转化生长因子(transforming growth factorβ1,TGFβ1)的变化,并探讨其在CML的诊治及预后判定中的临床意义。方法:以在安康市中心医院就诊的50例初诊患者(其中慢性期30例,加速期10例,急变期10例)为研究对象,以该院体检中心健康体检者40例为正常对照,采用ELISA方法检测血清COX-2、b FGF及TGFβ1水平,并对CML患者进行治疗前、后两个不同阶段的动态检测。结果:初诊时,CML组患者的血清COX-2及b FGF含量显著升高,而TGFβ1含量明显减低(P0.05);未达到缓解时,血清b FGF、COX-2及TGFβ1水平与CR组比较差异显著(均为P<0.001)。CML初诊患者治疗前血清b FGF、VEGF水平与TGFβ1呈负相关(分别为r=-0.330,P=0.020;r=-0.632,P<0.001)。结论 :CML患者初诊时COX-2及b FGF高表达,TGFβ1低表达,经过甲磺酸伊马替尼治疗后,达到CR时,血清这些因子水平基本恢复正常,提示C ML的发生、发展与这些因子密切相关。因此,动态监测CML患者血清COX-2、b FGF及TGFβ1水平,可作为CML病情评估的重要参考指标。 展开更多
关键词 慢性粒细胞白血病 环氧合酶-2(COX-2) 碱性成纤维细胞生长因子(bFGF) 转化生长因子(TGFβ1)
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地西他滨联合小剂量HAG方案对急性髓系白血病患者细胞免疫功能及血清COX-2、bFGF的影响 认领 引用 被引量：9
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作者 安晋婷 马梁明 +2 位作者 王涛 田卫伟 贺少龙 《现代生物医学进展》 CAS 2021年第2期347-350,共4页
目的:探讨地西他滨联合小剂量高三尖杉酯碱+阿糖胞苷+重组人粒细胞集落刺激因子(HAG)方案对急性髓系白血病(AML)患者细胞免疫功能及血清环氧合酶-2(COX-2)、碱性成纤维细胞生长因子(bFGF)的影响。方法:选取2016年4月~2019年4月期... 目的:探讨地西他滨联合小剂量高三尖杉酯碱+阿糖胞苷+重组人粒细胞集落刺激因子(HAG)方案对急性髓系白血病(AML)患者细胞免疫功能及血清环氧合酶-2(COX-2)、碱性成纤维细胞生长因子(bFGF)的影响。方法:选取2016年4月~2019年4月期间山西白求恩医院收治的AML患者93例,根据随机数字表法分为对照组(n=46,HAG方案)和研究组(n=47,地西他滨联合小剂量HAG方案),比较两组患者临床疗效、细胞免疫功能、血清COX-2、bFGF水平的变化情况,记录两组治疗期间不良反应情况。结果:研究组治疗后的临床总有效率为80.85%(38/47),高于对照组的56.52%(26/46)(P0.05)。结论:地西他滨联合小剂量HAG方案治疗可减轻AML患者机体免疫抑制,改善血清COX-2、bFGF水平,且用药安全性较好。 展开更多
关键词 地西他滨 小剂量HAG方案 急性髓系白血病 细胞免疫 环氧合酶-2 碱性成纤维细胞生长因子
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BMP-2和bFGF在强直性脊柱炎活动期骶髂关节滑膜组织中的表达 认领 引用 被引量：16
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作者 李建明 初同伟 周跃 《第三军医大学学报》 北大核心 2008年第3期251-253,共3页
目的探讨骨形态发生蛋白2(bone morphogenetic protein-2,BMP-2)和碱性成纤维细胞生长因子(basic fibro-blast growth factor,bFGF)在强直性脊柱炎(ankylosing spondylitis,AS)活动期的病理性表达。方法通过免疫组化技术检测活动期AS患... 目的探讨骨形态发生蛋白2(bone morphogenetic protein-2,BMP-2)和碱性成纤维细胞生长因子(basic fibro-blast growth factor,bFGF)在强直性脊柱炎(ankylosing spondylitis,AS)活动期的病理性表达。方法通过免疫组化技术检测活动期AS患者与对照组(骨盆骨折患者)骶髂关节滑膜组织中BMP-2、bFGF的表达,用图像分析系统比较二者的表达差异。结果在AS活动期患者骶髂关节滑膜组织中BMP-2和bFGF为阳性表达,而对照组骶髂关节滑膜组织中BMP-2和bFGF为阴性表达,图像灰度值比较二者差异有统计学意义(P<0.01)。结论BMP-2和bFGF均为AS病理性成骨过程中重要的成骨因子,它们与强直性脊柱炎骶髂关节成骨硬化过程有密切关系。 展开更多
关键词 强直性脊柱炎 骨形态发生蛋白2 碱性成纤维细胞生长因子
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