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Emergence and molecular characterization of bovine papular stomatitis virus in Chinese cattle 认领 引用
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作者 Yue Sun Wenyuan Gu +3 位作者 Yiman Xue Zhuoya Li Changchun Tu Liqian Zhu 《Virologica Sinica》 SCIE CAS CSCD 2026年第1期227-230,共4页
Dear Editor,Bovine papular stomatitis virus(BPSV)belongs to the Parapoxvirus(PPVs)genus of the Poxviridae family(Proost et al.,2016).It causes bovine papular stomatitis(BPS),a non-systemic proliferative dermatosis tha... Dear Editor,Bovine papular stomatitis virus(BPSV)belongs to the Parapoxvirus(PPVs)genus of the Poxviridae family(Proost et al.,2016).It causes bovine papular stomatitis(BPS),a non-systemic proliferative dermatosis that affects both domestic and wild ruminants(Micheloud et al.,2020). 展开更多
关键词 bovine papular stomatitis bps poxviridae parapoxvirus papular stomatitis bovine papular stomatitis bovine papular stomatitis virus dermatosis
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Mitochondrial dysfunction in the bovine mammary gland:regulatory mechanisms and therapeutic strategies 认领 引用
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作者 Taiyu Shen Ming Li +5 位作者 Xudong Sun Zhaoju Deng Hailey Pitts Derek Nolan Juan J.Loor Chuang Xu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第3期1170-1185,共16页
Periparturient metabolic stress or pathogen infections leading to inflammation reduce milk yield and cause mammary dysfunction,thus,causing severe economic losses to dairy farming.As the primary organelle for cellular... Periparturient metabolic stress or pathogen infections leading to inflammation reduce milk yield and cause mammary dysfunction,thus,causing severe economic losses to dairy farming.As the primary organelle for cellular energy production,calcium regulation,cell death,and metabolism,mitochondrial homeostasis plays a crucial role in maintaining normal mammary gland function.This review focuses on the regulatory mechanisms of mitochondrial homeostasis and the effect of mitochondrial dysfunction on homeostatic mechanisms in bovine mammary gland.Furthermore,we summarize several potential therapeutic strategies targeting mitochondrial metabolic dysfunction to alleviate mammary tissue damage. 展开更多
关键词 Apoptosis Bovine Innate immunity Mammary gland homeostasis Reactive oxygen species
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Deciphering the dialogue between the bovine blastocyst and the uterus:embryo-induced alterations in extracellular vesicle protein content from an ex vivo model and the in vivo environment 认领 引用
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作者 Rosane Mazzarella Jose Maria Sanchez +10 位作者 Sandra Guisado Egido Michael McDonald Alberto Alvarez‑Barrientos Esperanza Gonzalez Juan Manuel Falcon-Perez Mikel Azkargorta Felix Elortza Maria Encina Gonzalez Pat Lonergan Dimitrios Rizos Beatriz Fernandez-Fuertes 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期213-240,共28页
Backgroud Efficient communication between the embryo and the endometrium is essential for the successful establishment and maintenance of pregnancy.Uterine-derived extracellular vesicles(EVs)contribute to embryomatern... Backgroud Efficient communication between the embryo and the endometrium is essential for the successful establishment and maintenance of pregnancy.Uterine-derived extracellular vesicles(EVs)contribute to embryomaternal communication,supporting early embryonic development.This study aimed to:(i)compare the protein cargo of uterine fluid EVs(UF-EVs)from CYCLIC and PREGNANT heifers;(ii)characterize the protein profile of conditioned medium(CM)-EVs from endometrial explants cultured alone(EXPL)or co-cultured with five d 7 blastocysts(EXPL+EMB)in vitro;and(iii)compare the EV protein cargo between the in vivo and in vitro models(i.e.,EXPL vs.CYCLIC and EXPL+EMB vs.PREGNANT).Results We identified 1,459 and 1,752 proteins in the UF-EVs of CYCLIC and PREGNANT heifers,respectively.Among these,12 were exclusive to CYCLIC,and 18 were exclusive to PREGNANT.Among the 1,329 proteins identified in both groups,16 were differently abundant;ten were more abundant,and six were less abundant in UF-EVs from PREGNANT heifers.In vivo,the changes in UF-EV protein cargo induced by the presence of a blastocyst were related to inflammatory and immune responses,endometrial receptivity,and support of early embryonic development by promoting cell polarity,cell–cell adhesion,and stem cell differentiation.In vitro,we identified 1,501 proteins in the CM-EVs from EXPL,1,975 in the CM-EVs from EXPL+EMB,and 82 in the CM-EVs from EMB.Additionally,50 proteins were unique to EXPL+EMB,and another 33 were differentially abundant due to the synergistic interaction between the embryo and the endometrium.These proteins are involved in embryonic development,regulation of stem cell differentiation,establishment and maintenance of cell polarity,interferon tau(IFNT)-mediated cell signaling,endometrial receptivity,and immune modulation.Although there are qualitative and quantitative differences between in vivo and in vitro-derived EVs,UF-EVs from CYCLIC heifers compared to CM-EVs from EXPL,as well as UFEVs from PREGNANT heifers compared to CM-EVs from EXPL+EMB shared common proteins.Conclusions These findings highlight the pivotal role of EVs in embryo-maternal communication,suggesting that their protein cargo may actively contribute to the modulation of the uterine environment to support early embryonic development.Understanding these molecular interactions could provide valuable insights into the mechanisms of implantation and pregnancy establishment. 展开更多
关键词 Bovine blastocysts Early pregnancy Embryonic extracellular vesicles Embryo-maternal communication Endometrial explants Proteomics Uterine fluid
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ACSL4 is a target forβ-hydroxybutyrate-induced increase in fatty acid content and lipid droplet accumulation in bovine mammary epithelial cells 认领 引用
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作者 Ming Li Jingjing Wang +8 位作者 Jia’nan Wen Juan JLoor Qianming Jiang Jingyi Wang Huijing Zhang Yue Yang Wei Yang Bingbing Zhang Chuang Xu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第3期1137-1149,共13页
Ketosis,a common metabolic disease during early lactation,is associated with high circulating levels ofβ-hydroxybutyrate(BHB).A portion of BHB that reaches the mammary gland is utilized as precursor for synthesis of ... Ketosis,a common metabolic disease during early lactation,is associated with high circulating levels ofβ-hydroxybutyrate(BHB).A portion of BHB that reaches the mammary gland is utilized as precursor for synthesis of fatty acids.Recent findings from nonruminant studies revealed that long chain fatty acyl-CoA ligase 4(ACSL4)could play a role in the regulation of cellular fatty acid metabolism,but the mechanisms by which ACSL4 mediates cellular lipid metabolism in response to BHB remains unclear.To achieve the aims,we conducted in vivo or in vitro analyses using bovine mammary gland biopsies and the immortalized mammary epithelial cell line(MAC-T).The in vivo study(n=6 cows per group)involved healthy cows(plasma BHB2.0 mmol L–1)from which mammary gland tissue was biopsied.In vitro,MAC-T cells were challenged with 0,0.3,0.6,1.2,or 2.4 mmol L–1BHB for 24 h to determine an optimal dose.Subsequently,MAC-T were incubated with 1.2 mmol L–1BHB for 0,3,6,12,24,or 48 h.Furthermore,MAC-T cells were treated with small interfering ACSL4(siACSL4)for 24 h or ACSL4 overexpression plasmid(pcACSL4)for 36 h followed by a challenge with 1.2 mmol L–1BHB for 24 h.Results showed that increased mRNA and protein abundance of lipogenic genes were linked to both mammary gland and in vitro challenge with BHB.BHB increased fatty acid content by activating ACSL4 expression,whereas inhibition of ACSL4 reduced BHB-induced reactive oxygen species(ROS)overproduction,enhancement of mitochondrial membrane potential,increase in fatty acid content,and lipid droplet accumulation.Furthermore,we also elevated ACSL4 expression with an overexpression plasmid to clarify its molecular role in response to BHB challenge.ACSL4 overexpression enhances BHB-induced lipid droplet accumulation by increased fatty acid content.Overall,the information showed that ACSL4 is crucial for the process of producing fatty acids from exogenous BHB.Reduced ACSL4 decreased fatty acid content and lipid droplet accumulation,improved mitochondrial function,directed more fatty acids towards oxidation.Thus,ACSL4 plays an important role in determining the fate of intracellular fatty acids and BHB in BMECs. 展开更多
关键词 bovine mammary epithelial cells fatty acid content ketosis BHB ACSL4
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Bovine Serum Albumin over Graphene Oxide Surface:Binding Capacity and Interaction 认领 引用
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作者 RUPALI Pandey SANDEEP Singhania +3 位作者 AN JU RAGINI Chaturvedi RAJESH Chaudhary AMIT Garg 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2026年第4期1159-1165,共7页
We investigated the binding of universal protein BSA(Bovine Serum Albumin)over graphene oxide(GO)surface and performed quantitative measurements to find its maximum binding capacity.The interaction of proteins onto a ... We investigated the binding of universal protein BSA(Bovine Serum Albumin)over graphene oxide(GO)surface and performed quantitative measurements to find its maximum binding capacity.The interaction of proteins onto a 2D surface can happen via two available orthogonal extremes-side-on and end-on.Electrochemical measurements using cyclic voltammetry to define the uniqueness of the present study as it confirms the binding of BSA over GO surface and evaluate its maximum capacity.The reliability of the results is verified by repeating the experiment multiple times over a year.UV-Vis,FTIR,Raman,and SEM measurements clearly show that BSA is perfectly bound over GO.The estimated value of BSA over GO calculated by standard protein estimation test-Lowry’s protein assay is 1.1 mg/mg. 展开更多
关键词 Graphene oxide Bovine Serum Albumin Binding capacity Lowry’s protein assay Cyclic voltammetry
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Pulsed electric fields-mediated inactivation of foodborne pathogens in bovine colostrum 认领 引用
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作者 Jessie King Madeleine Tess Hardie Boys +5 位作者 Daniel Pletzer Jo Finer Cissie Chen Leon Fung Phil Bremer Indrawati Oey 《Food Innovation and Advances》 2026年第1期45-54,共10页
Bovine colostrum exhibits promising immunological properties,but the degradation of immunoglobulins during conventional thermal pasteurization limits its widespread use.Pulsed electric fields(PEF)processing,with its m... Bovine colostrum exhibits promising immunological properties,but the degradation of immunoglobulins during conventional thermal pasteurization limits its widespread use.Pulsed electric fields(PEF)processing,with its minimal thermal effect,is a promising alternative pasteurization technology.This study explored the potential of using PEF processing to inactivate bacteria in bovine colostrum.The effect of continuous flow PEF on the inactivation of naturally occurring bacteria in early(0-48 h lactation)and late(≤7 d lactation)stage colostrum was tested.Preheating to 45℃combined with PEF treatment(~13 kV/cm,229-239 kJ/L)resulted in a>5-log reduction in microbial numbers for both conditions.Next,the feasibility of using PEF to inactivate surrogate non-pathogenic organisms,Escherichia coli ATCC 25922 and Listeria innocua,was investigated.Following 40℃pre-heating and PEF treatment(11 kV/cm,209 kJ/L),a 5-log reduction was achieved,though L.innocua appeared less sensitive to PEF treatment.Finally,the effect of PEF on pathogenic bacteria was explored in batch mode,where samples were contained within cuvettes.Colostrum was inoculated with two cocktails of either three pathogenic E.coli or five L.monocytogenes strains.At a field strength of 8 kV/cm and pre-heating to 40℃,maximum specific energies of 184 and 175 kJ/kg resulted in 4-and 2.4-log reductions in E.coli and L.monocytogenes,respectively,further supporting the different sensitivities of bacteria to PEF treatment.Based on current knowledge,this is the first study to demonstrate the feasibility of PEF as an alternative pasteurization technology for colostrum. 展开更多
关键词 Pulsed Electric Fields inactivate bacteria conventional thermal pasteurization Colostrum Pasteurization Bacterial Inactivation naturally occurring bacteria electric fields pef processingwith bovine colostrumthe
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Dissecting the development of bovine testicular tissue using spatial transcriptomics 认领 引用
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作者 Haoyan Jin Yuan Ma +5 位作者 Yaru Xie Shunkai Yang Xiaoxu Chen Nana Wang Lingkai Zhang Yun Ma 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期749-767,共19页
Background Mammalian spermatogenesis is critical for the transmission of male genetic information,and singlecell sequencing technology can reveal its complex process.However,at present,there is no research on the dyna... Background Mammalian spermatogenesis is critical for the transmission of male genetic information,and singlecell sequencing technology can reveal its complex process.However,at present,there is no research on the dynamic transcription of bovine germ cell population.Results In this study,we used Stereo-seq to construct a spatial transcription map of bovine testicular tissue at two ages.Four germ cell groups and five somatic cell groups were determined,and functional enrichment characterized their different biological functions and the differences between calves and adult bulls.At the same time,we also defined the subpopulations of cells and marker genes,then,clarified the communications between germ cells.Conclusion Our study constructed a spatial transcription map of bovine testicular tissue for the first time,and systematically described the dynamic transcription changes during spermatogenesis.These data laid the foundation for the study of spermatogenesis in large mammals and elucidated the transcriptional dynamics underlying male germ cell development. 展开更多
关键词 Bovine Spatial transcriptome Spermatogenesis
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Network Pharmacological and Molecular Mechanism Analysis of Bovine Embryonic Stem Cell Mitochondria Polypeptides(EST-Mips)in Anti-Aging 认领 引用
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作者 Junjie Hao Pengcheng Ding +1 位作者 Huoqiang Jiang Jiren Zhang 《Journal of Clinical and Nursing Research》 2026年第6期362-374,共13页
Embryonic stem cell mitochondrial peptides(EST-Mips)are a group of peptide molecules derived from bovine embryonic stem cells,closely associated with telomere maintenance and mitochondrial function.In this study,based... Embryonic stem cell mitochondrial peptides(EST-Mips)are a group of peptide molecules derived from bovine embryonic stem cells,closely associated with telomere maintenance and mitochondrial function.In this study,based on 736 EST-Mips-regulated protein targets provided by users,we employed network pharmacology and bioinformatics approaches to systematically elucidate their molecular mechanisms in delaying the aging process.By screening target-aging associations using platforms such as PubChem,GeneCards,Human Aging Genomic Resources(HAGR/GenAge),Open Genes,and AgeAnno,we identified a total of 179 shared aging-related targets(accounting for 24.3%of all targets).Key targets include hub proteins with node connectivity≥50,such as TP53,AKT1,SRC,EGFR,TNF,IL6,MAPK1,and MAPK3.GO functional enrichment analysis revealed that EST-Mips primarily participate in cell cycle regulation,apoptosis signaling,oxidative stress response,and inflammatory responses.KEGG pathway analysis demonstrated that EST-Mips exert their anti-aging effects by modulating aging-related signaling pathways,including PI3K-Akt,FoxO,p53,MAPK,JAK-STAT,and NF-κB.Comprehensive analysis indicates that EST-Mips exert their anti-aging effects through a multidimensional network involving“telomere maintenance—mitochondrial homeostasis—metabolic remodeling—immune modulation”synergistically. 展开更多
关键词 Bovine embryonic brain cells Mitochondrial polypeptides Network pharmacology Molecular docking Anti-aging
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Bovine colostrum for prevention of recurrent urinary tract infections in susceptible children:A randomized controlled trial 认领 引用
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作者 Ragia M Said Abanoub A Wissa +1 位作者 Sara T Wahib Noha U Hashem 《World Journal of Clinical Pediatrics》 2026年第3期327-336,共10页
BACKGROUND Recurrent urinary tract infections(UTIs)in children with congenital anomalies of the kidney and urinary tract(CAKUT)are a major cause of deterioration of kidney functions and possibly,renal scarring.Rising ... BACKGROUND Recurrent urinary tract infections(UTIs)in children with congenital anomalies of the kidney and urinary tract(CAKUT)are a major cause of deterioration of kidney functions and possibly,renal scarring.Rising antimicrobial resistance necessitates alternative preventive strategies.Bovine colostrum(BC),a bioactive prebiotic,has emerged as a promising adjuvant therapy in upper respiratory and gastrointestinal(GIT)infections.Given the shared mucosal immune mechanisms,the urinary tract may represent a suitable target for this preventive strategy to reduce UTI recurrence.AIM To evaluate the potential role of oral BC as an adjuvant therapy for preventing recurrent UTIs in children with CAKUT.METHODS This randomized,double-blinded,placebo-controlled trial enrolled 60 children(0.05).Colostrum supplementation showed a nonsignificant reduction in the overall incidence of upper and lower UTIs vs placebo over six months[relative risk(RR)=0.84,95%CI:0.63-1.12;P=0.22].However,significant reductions of 73%-75%in UTI incidence were observed during the fourth,fifth,and sixth months of follow up(P<0.05 for each).When stratified by infection type,cystitis incidence was significantly lower in the colostrum group(43.3%vs 70.0%;RR=0.62;95%CI:0.39-0.99;P=0.037),while no significant difference was observed in pyelonephritis.GTI and respiratory infections were reduced by 34%(RR=0.66;95%CI:0.50-0.87;P=0.001).No allergic reactions were reported.CONCLUSION BC supplementation may reduce cystitis,as well as respiratory and GIT infections,in children with CAKUT.The observed month-specific reduction in UTIs suggests a possible cumulative benefit with continued supplementation,warranting further research. 展开更多
关键词 Recurrent urinary tract infections Respiratory tract infections Gastrointestinal infections Children Bovine colostrum
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Enzootic bovine leukosis in Indonesia:Current evidence,knowledge gaps,and future research directions 认领 引用
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作者 Arif Nur Muhammad Ansori Maxim Petropavlovskiy +1 位作者 Irina Donnik Maksim Rebezov 《Infection Advances》 2026年第2期17-22,共6页
Enzootic bovine leukosis(EBL)is a chronic lymphoproliferative disease caused by bovine leukemia virus(BLV),a retrovirus that establishes lifelong infection in cattle.While sustained surveillance and control programs h... Enzootic bovine leukosis(EBL)is a chronic lymphoproliferative disease caused by bovine leukemia virus(BLV),a retrovirus that establishes lifelong infection in cattle.While sustained surveillance and control programs have led to successful eradication in several European countries,BLV remains endemic across much of Asia,the Americas,and Africa.Infection is typically asymptomatic;however,a small proportion of infected animals develop malignant lymphoma,resulting in significant economic losses and animal welfare concerns.In Indonesia,understanding of BLV epidemiology remains notably limited.Existing data are derived primarily from small-scale or localized studies,providing an incomplete and fragmented picture of national prevalence and viral genetic diversity.Assessing BLV transmission dynamics is further complicated by the heterogeneous structure of Indonesia’s cattle industry,which is largely dominated by smallholder beef systems utilizing diverse indigenous breeds,alongside a more centralized and intensive dairy production sector.This paper synthesizes current knowledge on BLV biology and global epidemiological patterns,while critically evaluating the sparse evidence available from Indonesia.Key gaps are identified in risk factor characterization,molecular surveillance,and national-level monitoring frameworks.In the absence of effective vaccines,strengthening integrated and systematic surveillance systems is essential for informing evidence-based control strategies and developing a coherent national policy for BLV mitigation in Indonesia. 展开更多
关键词 enzootic bovine leukosis epidemiology Deltaretrovirus Indonesia veterinary medicine
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Functional validation to explore the protective role of miR-223 in Staphylococcus aureus-induced bovine mastitis 认领 引用 被引量：3
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作者 Xueqin Liu Siyuan Mi +4 位作者 Gerile Dari Siqian Chen Jiuzhou Song David E.MacHugh Ying Yu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第3期1009-1024,共16页
Background Mastitis caused by Staphylococcus aureus(S.aureus)is one of the most intractable problems for the dairy industry,causing significantly reduced milk yields and early slaughter of cows worldwide.MicroRNAs(miR... Background Mastitis caused by Staphylococcus aureus(S.aureus)is one of the most intractable problems for the dairy industry,causing significantly reduced milk yields and early slaughter of cows worldwide.MicroRNAs(miRNAs)can post-transcriptionally regulate gene expression and studies in recent years have shown the importance of miRNA-associated gene regulation in S.aureus-induced mastitis.Results In this study,to investigate the role of miR-223 in mastitis,we performed experiments to overexpress and suppress miR-223 in an immortalized bovine mammary epithelial cell line(MAC-T)infected with S.aureus.Overexpression of miR-223 in MAC-T cells repressed cell apoptosis and necrosis induced by S.aureus infection,whereas suppression of miR-223 had the opposite effect.Transcriptome expression profiling with weighted gene co-expression network analysis(WGCNA)and gene set variation analysis(GSVA)showed that miR-223 affects apoptosis and inflammation-related pathways.Furthermore,differentially expressed(DE)genes were evaluated,and genes exhibiting contrasting expression trends in the miR-223 overexpressed and suppressed groups were assessed as potential target genes of miR-223.Potential target genes,including CDC25B,PTPRF,DCTN1,and DPP9,were observed to be associated with apoptosis and necroptosis.Finally,through integrative analysis of genome-wide association study(GWAS)data and the animal quantitative trait loci(QTL)database,we determined that target genes of miR-223 were significantly enriched in single-nucleotide polymorphisms(SNP)and QTLs related to somatic cell count(SCC)and mastitis.Conclusion In summary,miR-223 has an inhibitory effect on S.aureus-induced cell apoptosis and necrosis by regulating PTPRF,DCTN1,and DPP9.These genes were significantly enriched in QTL regions associated with bovine mastitis resistance,underscoring their relevance in genetic regulation of disease resilience.Our findings provide critical genetic markers for enhancing mastitis resistance,particularly S.aureus-induced mastitis,through selective breeding.This work offers valuable insights for developing cattle with improved resistance to mastitis via targeted genetic selection. 展开更多
关键词 Bovine mastitis Gene regulation Mammary epithelial cells MiR-223 Staphylococcus aureus
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β‑Hydroxybutyrate‑induced mitochondrial DNA (mtDNA) release mediated innate inflammatory response in bovine mammary epithelial cells by inhibiting autophagy 认领 引用 被引量：2
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作者 Yihui Huo Taiyu Shen +6 位作者 Tianyin Feng Moli Li Wanli Zhao Juan JLoor Ben Aernouts Androniki Psifidi Chuang Xu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第2期653-667,共15页
Background In perinatal dairy cows,ketosis is a prevalent metabolic disorder that lowers milk output and per-formance.Mitochondrial dysfunction and chronic inflammation in mammary tissue are linked to elevated blood k... Background In perinatal dairy cows,ketosis is a prevalent metabolic disorder that lowers milk output and per-formance.Mitochondrial dysfunction and chronic inflammation in mammary tissue are linked to elevated blood ketone levels,particularlyβ-hydroxybutyrate(BHB).Recent research has linked cytosolic mitochondrial DNA(mtDNA)with chronic aseptic inflammation by activating the cGAS-STING pathway during metabolic disorders,while autophagy activation effectively reverses this process.However,whether it is involved in mammary gland damage during ketosis is poorly understood.Therefore,this study aimed to explore the underlying mechanisms of mtDNA-induced inflammation under BHB stress and evaluate the potential therapeutic strategy of autophagy activation in mitigating this damage.Results Our study found an increased cytoplasmic mtDNA abundance in mammary gland tissues of dairy cows with ketosis and bovine mammary epithelial cell line(MAC-T)subjected to BHB stress.Further investigations revealed the activation of the cGAS-STING pathway and inflammatory response,indicated by elevated levels of cGAS and STING,along with increased phosphorylation levels of TBK1,P65,and IκB,and higher transcript levels of pro-inflammatory factors(IL-1B,IL-6,and TNF-α)in both in vivo and in vitro experiments.Notably,STING inhibition via si-STING transfection reversed BHB-induced inflammation.Additionally,autophagy activation appeared to protect against BHB stress by facilitating the removal of cytoplasmic mtDNA and preventing cGAS-STING pathway-mediated inflammation.Conclusions The findings illustrate that elevated BHB levels lead to the release of cytoplasmic mtDNA,which in turn activates the cGAS-STING pathway and triggers an inflammatory response in the mammary glands during hyper-ketonemia.Conversely,autophagy activation has been shown to alleviate this process by promoting cytoplasmic mtDNA degradation. 展开更多
关键词 Autophagy Bovine mammary gland Inflammation Mitochondria DNA
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Comprehensive characterization of lncRNA N6-methyladenosine modification dynamics throughout bovine skeletal muscle development 认领 引用 被引量：2
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作者 Cui Mao Wei You +4 位作者 Yuta Yang Haijian Cheng Xin Hu Xianyong Lan Enliang Song 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第3期1025-1040,共16页
Background N6-methyladenosine(m6A)methylation is a key epigenetic modification that can modulate gene expression and strongly affect mammalian developmental processes.However,the genome-wide methylation of long ... Background N6-methyladenosine(m6A)methylation is a key epigenetic modification that can modulate gene expression and strongly affect mammalian developmental processes.However,the genome-wide methylation of long non-coding RNAs(lncRNAs)and its implications for the development of skeletal muscle remain poorly understood.Bovine skeletal muscle samples from five developmental stages were analyzed in this study to establish lncRNA methylome and transcriptomic maps.Results Globally,59.67%of lncRNAs in skeletal muscle with m6A modifications,and this percentage decreased progressively during development.lncRNA expression levels were positively associated with the number of m6A peaks,with lncRNAs possessing 3 or more peaks showing significantly higher expression levels than those with 1 or 2 peaks.Specific lncRNAs involved in skeletal muscle development were identified through two analytical approaches.The first approach employed weighted gene co-expression network analysis(WGCNA)of transcriptomic data to identify correlations between annotated lncRNAs and growth-related traits,resulting in 21 candidate hub lncRNAs.The intersection of these 21 hub lncRNAs with 151 differentially methylated lncRNAs(DM-lncRNAs)identified 10 shared candidate lncRNAs.The second approach integrated MeRIP-seq and RNA-seq data to identify 36 lncRNAs that were both differentially m6A modified and differentially expressed(dme-lncRNAs).GO and KEGG enrichment analyses of cis-target genes associated with these dme-lncRNAs identified eight candidate lncRNAs.Combining the results from the two approaches identified 16 key m6A-modified lncRNAs likely involved in skeletal muscle development.Conclusions These findings highlight the regulatory and functional significance of dynamic lncRNA methylation in skeletal muscle development. 展开更多
关键词 Bovine LncRNA m6A methylation Muscle development
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Bovine milk-derived extracellular vesicles reduce oxidative stress and ferroptosis induced by Klebsiella pneumoniae in bovine mammary epithelial cells 认领 引用 被引量：1
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作者 Bingchun Liang Yindi Xiong +4 位作者 Eduardo R.Cobo John Kastelic Xiaofang Tong Bo Han Jian Gao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第3期1129-1141,共13页
Background Ferroptosis is characterized by increased production of reactive oxygen species(ROS)and membrane lipid peroxidation that can exacerbate inflammatory damage.Extracellular vesicles(EVs)isolated from bovine mi... Background Ferroptosis is characterized by increased production of reactive oxygen species(ROS)and membrane lipid peroxidation that can exacerbate inflammatory damage.Extracellular vesicles(EVs)isolated from bovine milk have many biological functions,including antioxidant properties.However,the role of EVs on Klebsiella pneumoniaeinduced ferroptosis and oxidative stress in bovine mammary epithelial cells(bMECs)and murine mammary tissue is unclear.In this study,EVs were isolated from bovine colostrum,mature milk and clinical mastitis milk(defined as C-EVs,M-EVs and CM-EVs,respectively)and assessed by transmission electron microscopy,Western blot and transcriptome sequencing.Effects of EVs on K.pneumoniae-induced ferroptosis and oxidative stress in bMECs were evaluated with immunofluorescence and Western blot.Results In bMECs,infection with K.pneumoniae induced oxidative stress,decreasing protein expression of Nrf2,Keap1 and HO-1 plus SOD activity,and increasing ROS concentrations.However,protein expression of GPX4,ACSL4 and S100A4 in bMECs,all factors that regulate ferroptosis,was downregulated by K.pneumoniae.Furthermore,this bacterium compromised tight junctions in murine mammary tissue,with low expression of ZO-1 and Occludin,whereas protein expression of Nrf2 and GPX4 was also decreased in mammary tissue.Adding C-EVs,M-EVs or CMEVs reduced oxidative stress and ferroptosis in K.pneumoniae-infected bMECs in vitro and murine mammary tissues in vivo.Conclusion In conclusion,all 3 sources of milk-derived EVs alleviated oxidative stress and ferroptosis in K.pneumoniae-infected bMECs and mammary tissues. 展开更多
关键词 Bovine mammary epithelial cells Extracellular vesicles Ferroptosis Klebsiella pneumoniae Oxidative stress
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Maternal stress and the early embryonic microenvironment:investigating long‑term cortisol effects on bovine oviductal epithelial cells using air–liquid interface culture 认领 引用
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作者 Fiona Wahl Jianchao Huo +2 位作者 Shuaizhi Du Jennifer Schoen Shuai Chen 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期153-166,共14页
The oviduct epithelium is the initial maternal contact site for embryos after fertilization,offering the microenviron-ment before implantation.This early gestation period is particularly sensitive to stress,which can ... The oviduct epithelium is the initial maternal contact site for embryos after fertilization,offering the microenviron-ment before implantation.This early gestation period is particularly sensitive to stress,which can cause reduced fertil-ity and reproductive disorders in mammals.Nevertheless,the local impact of elevated stress hormones on the ovi-duct epithelium has received limited attention to date,except for a few reports on polyovulatory species like mice and pigs.In this study,we focused on the effects of chronic maternal stress on cattle,given its association with infertil-ity issues in this monoovulatory species.Bovine oviduct epithelial cells(BOEC)differentiated at the air–liquid interface(ALI)were stimulated with 250 nmol/L cortisol for 1 or 3 weeks.Subsequently,they were assessed for morphology,bioelectrical properties,and gene expression related to oviduct function,glucocorticoid pathway,cortisol metabo-lism,inflammation,and apoptosis.Results revealed adverse effects of cortisol on epithelium structure,featured by deciliation,vacuole formation,and multilayering.Additionally,cortisol exposure led to an increase in transepithelial potential difference,downregulated mRNA expression of the major glucocorticoid receptor(NR3C1),upregulated the expression of cortisol-responsive genes(FKBP5,TSC22D3),and significant downregulation of oviductal glycopro-tein 1(OVGP1)and steroid receptors PGR and ESR1.The systematic comparison to a similar experiment previously performed by us in porcine oviduct epithelial cells,indicated that bovine cultures were more susceptible to elevated cortisol levels than porcine.The distinct responses between both species are likely linked to their divergence in the cortisol-induced expression changes of HSD11B2,an enzyme controlling the cellular capacity to metabolise cortisol.These findings provide insights into the species-specific reactions and reproductive consequences triggered by maternal stress. 展开更多
关键词 Air–liquid interface Bovine Cortisol Early embryonic microenvironment Epithelial cells Maternal stress Oviduct
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Exosomes separation with aqueous two-phase systems from bovine milk 认领 引用
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作者 Jingjing Sun Ruoque Mao +2 位作者 Xiaoqian Fu Shanjing Yao Dongqiang Lin 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2025年第5期1-10,共10页
The exosomes hold significant potential in disease diagnosis and therapeutic interventions.The objective of this study was to investigate the potential of aqueous two-phase systems(ATPSs)for the separation of bovine m... The exosomes hold significant potential in disease diagnosis and therapeutic interventions.The objective of this study was to investigate the potential of aqueous two-phase systems(ATPSs)for the separation of bovine milk exosomes.The milk exosome partition behaviors and bovine milk separation were investigated,and the ATPSs and bovine milk whey addition was optimized.The optimal separation conditions were identified as 16%(mass)polyethylene glycol 4000,10%(mass)dipotassium phosphate,and 1%(mass)enzymatic hydrolysis bovine milk whey.During the separation process,bovine milk exosomes were predominantly enriched in the interphase,while protein impurities were primarily found in the bottom phase.The process yielded bovine milk exosomes of 2.0×1011particles per ml whey with high purity(staining rate>90%,7.01×1010particles per mg protein)and high uniformity(polydispersity index<0.03).The isolated exosomes were characterized and identified by transmission electron microscopy,zeta potential and size distribution.The results demonstrated aqueous two-phase extraction possesses a robust capability for the enrichment and separation of exosomes directly from bovine milk whey,presenting a novel approach for the large-scale isolation of exosomes. 展开更多
关键词 Aqueous two-phase extraction Bovine milk exosomes Separation Purification
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A Quantitative Assay for Measuring of Bovine Immunodeficiency Virus Using a Luciferase-based Indicator Cell Line 认领 引用
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作者 Xue YAO Hong-yan GUO +5 位作者 Chang LIU Xuan XU Jian-sen DU Hao-yue LIANG Yun-qi GENG Wen-tao QIAO 《Virologica Sinica》 CAS 2010年第2期137-144,共8页
In order to quantitate the bovine immunodeficiency virus line （BIVL） was established by transfecting baby hamster kidney （BIV） cells infection in vitro, a BIV indicator cell with reporter plasmids containing the f... In order to quantitate the bovine immunodeficiency virus line （BIVL） was established by transfecting baby hamster kidney （BIV） cells infection in vitro, a BIV indicator cell with reporter plasmids containing the firefly luciferase gene driven by a BIV long terminal repeat promoter. The BIV activates promoter activity of the LTR to express luciferase upon infection. BIV infection could therefore by quantified by detection of luciferase activity. Compared to standard assays used to detect BIV infection, the BIVL-based assay is 10 times more sensitive than the the CPE-based assay, and has similar sensitivity with the viral capsid protein Western blot assay BIV indicator cell line could detect BIV infection specifically. Luciferase activity of BIV infected BIVL cells showed a time dependent manner, and 60 h post infection is the optimal time to detect BIV infection. Luciferase activity of BIVL cells correlates with the BIV capsid protein expression. Moreover, a linear relationship was found between MOI and the activated intensity of luciferase expression. In brief, the BIV indicator cell line is an easy, robust and quantitive method for monitoring BIV infection. 展开更多
关键词 Bovine immunodeficiency virus （BIV） Bovine foamy virus （BFV） Luciferase Indicator cell line
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Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway 认领 引用 被引量：19
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作者 Hai-na GAO Han HU +1 位作者 Nan ZHENG Jia-qi WANG 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2015年第6期560-572,共13页
The aim of this study is to investigate the effects of leucine（Leu） and histidine（His） on the expression of both the mammalian target of rapamycin（mTOR） signaling pathway-related proteins and caseins in immortal... The aim of this study is to investigate the effects of leucine（Leu） and histidine（His） on the expression of both the mammalian target of rapamycin（mTOR） signaling pathway-related proteins and caseins in immortalized bovine mammary epithelial cells（CMEC-H）, using a single supplement through Western blotting. The Earle＇s balanced salt solution（EBSS） was set as the control group and other treatment groups, based on the EBSS, were added with different concentrations of Leu or His, respectively. The results showed that, compared with the control group, the expression of caseins and the phosphorylation of mTOR（Ser^2481）, Raptor（Ser^792）, e IF4E（Ser^209）, and e EF2（Thr^56） increased with the Leu concentrations ranging from 0.45 to 10.80 mmol/L（P〈0.01）. The P-4EBP1（Thr^37） at 10.80 mmol/L Leu, and P-RPS6（Ser^235/236） at 5.40 to 10.80 mmol/L Leu all decreased. Similarly, the His supplementation from 0.15 to 9.60 mmol/L increased the expression of αs2-casein, β-casein, κ-casein, P-mTOR（Ser^2481）, P-Raptor（Ser^792）, P-S6K1（Thr^389）, P-4EBP1（Thr^37）, P-e IF4E（Ser^209）, and P-e EF2（Thr^56）（P〈0.01） in CMEC-H, whereas the αs1-casein expression was only reduced at 9.60 mmol/L His, G protein β subunit-like protein（GβL） at 0.15 and 9.60 mmol/L His, and P-RPS6 at 4.80 to 9.60 mmol/L His. Our linear regression model assay suggested that the αs1-casein expression was positively correlated with P-mTOR（P〈0.01）, P-S6K1（P〈0.01）, and P-e EF2（P〈0.01） for the addition of Leu, while the expressions of β-casein（P〈0.01） and κ-casein（P〈0.01） were positively correlated with P-e EF2 for the addition of His. In conclusion, the milk protein synthesis was up-regulated through activation of the mTOR pathway with the addition of Leu and His in CMEC-H. 展开更多
关键词 Bovine mammary epithelial cells Leucine Histidine Western blotting mTOR Casein
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Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China 认领 引用 被引量：14
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作者 Jian-ping LI Hai-jian ZHOU +2 位作者 Lin YUAN Ting HE Song-hua HU 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS 2009年第10期753-760,共8页
This study was conducted to determine genetic diversity and antimicrobial susceptibility profiles of Staphylococcus aureus recovered from bovine mastiffs in Zhejiang Province, China. Out of 3178 quarter milk samples f... This study was conducted to determine genetic diversity and antimicrobial susceptibility profiles of Staphylococcus aureus recovered from bovine mastiffs in Zhejiang Province, China. Out of 3178 quarter milk samples from 846 lactating cows, among which 459 cows （54.3%） were found HMT positive, 890 quarters （28%） were found having subclinical mastitis. From 75 representative S. aureus isolates, 16 distinct types were identified by pulsed-field gel electrophoresis （PFGE）. Four major PFGE types （A, B, C, and D） accounted for 82.7% of all isolates, and type A （41.3%） was observed in multiple herds across the studied areas. Each region was found to have a predominant type： Hangzhou type A （64.1%）, Ningbo type C （34.5%） and type B （23.1%）, Jinhua type D （53.3%）, and Taizhou type C （62.5%）. Results of antimicrobial susceptibility tests showed that 90.7% of the isolates were resistant to at least one antimicrobial. Resistance to penicillin and ampicillin （77.3%）, tetracycline （60.0%）, or erythromycin （48.0%） was observed. The bacteria resistant to multiple antibiotics such as penicillin, ampicillin, tetracycline, and erythromycin were commonly found. The information obtained from this study is useful for designing specific control programs for bovine S. aureus mastitis in this region. 展开更多
关键词 Staphylococcus aureus Bovine mastitis Antimicrobial resistance Genetic diversity Pulsed-field gel electrophoresis
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Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury 认领 引用 被引量：13
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作者 An LYU Jia-jia CHEN +5 位作者 Hui-chuan WANG Xiao-hong YU Zhi-cong ZHANG Ping GONG Lin-shu JIANG Feng-hua LIU 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2017年第6期481-491,共11页
Objective：Bovine endometritis is one of the most common reproductive disorders in cattle.The aim of this study was to investigate the anti-inflammation potential of punicalagin in lipopolysaccharide（LPS）-induced bo... Objective：Bovine endometritis is one of the most common reproductive disorders in cattle.The aim of this study was to investigate the anti-inflammation potential of punicalagin in lipopolysaccharide（LPS）-induced bovine endometrial epithelial cells（bE ECs）and to uncover the underlying mechanisms.Methods：bE ECs were stimulated with different concentrations（1,10,30,50,and 100μg/ml）of LPS for 3,6,9,12,and 18 h.MTT assay was used to assess cell viability and to identify the conditions for inflammatory injury and effective concentrations of punicalagin.Quantitative real-time polymerase chain reaction（q RT-PCR）was used to assess gene expression of pro-inflammatory cytokines.Western blotting was used to assess levels of inflammation-related proteins.Results：Treatment of b EECs with 30μg/ml LPS for 12 h induced cell injury and reduced cell viability.Punicalagin（5,10,or 20μg/ml）pretreatment significantly decreased LPS-induced productions of interleukin（IL）-1β,IL-6,IL-8,and tumor necrosis factor-α（TNF-α）in bE ECs.Molecular research showed that punicalagin inhibited the activation of the upstream mediator nuclear factor-κB（NF-κB）by suppressing the production of inhibitorκBα（IκBα）and phosphorylation of p65.Results also indicated that punicalagin can suppress the phosphorylation of mitogen-activated protein kinases（MAPKs）including p38,c-Jun N-terminal kinase（JNK）,and extracellular signal-regulated kinase（ERK）.Conclusions：Punicalagin may attenuate LPS-induced inflammatory injury and provide a potential option for the treatment of dairy cows with Escherichia coli endometritis. 展开更多
关键词 Bovine endometrial epithelial cell Cytokine Inflammatory injury Punicalagin
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