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Two aquaporins,LcPIP1;4 and LcPIP1;4a,cooperatively regulate the onset of dormancy of the terminal buds in evergreen perennial litchi(Litchi chinensis Sonn.) 认领 引用 被引量：1
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作者 Xue Tian Zhi-Qun Zhong +8 位作者 Yu Qi Meng-Meng Ma Ming-Chao Yang Dong-Cheng Li Fang-Yi Zhang Hui-Cong Wang Ji-Yuan Shen Ren-Fang Zeng Xu-Ming Huang 《Horticulture Research》 SCIE CSCD 2025年第8期146-163,共18页
Although extensively studied in various plants,the roles of aquaporin proteins in litchi remain unclear.In this study,low moisture content was observed in the dormant terminal buds of litchi.Transcriptome analysis rev... Although extensively studied in various plants,the roles of aquaporin proteins in litchi remain unclear.In this study,low moisture content was observed in the dormant terminal buds of litchi.Transcriptome analysis revealed that two aquaporin genes,PLASMA MEMBRANE INTRINSIC PROTEIN 1;4(LcPIP1;4)and LcPIP1;5,could be remarkably inhibited by exogenous ethylene(ETH),which also reduced the moisture content of litchi buds.Quantitative real-time polymerase chain reaction assays indicated that LcPIP1;4 expression was relatively elevated in the dormancy stage of litchi terminal buds.Inhibition of LcPIP1;4 in the buds of litchi during the growth stage delayed the onset of dormancy,resulting in a significantly reduced dormancy rate and increased moisture content.Further study indicated that LcPIP1;4 interacts with LcPIP1;4a,and they are capable of self-interaction.Silencing of LcPIP1;4a in litchi buds resulted in a phenotype consistent with silencing of LcPIP1;4.Additionally,simultaneous silencing of both LcPIP1;4 and LcPIP1;4a resulted in a more severe bud dormancy phenotype.Moreover,LcPIP1;4 was directly upregulated by LcRAP2.4.Silencing of LcRAP2.4 also delayed the onset of dormancy in litchi terminal buds,which is regulated by LcSVP2.ETH treatment at 1000 mg/l significantly downregulated the expression of LcPIP1;4 and LcRAP2.4,but had no significant effect on LcPIP1;4a.In contrast,abscisic acid(ABA)treatment at 200 mg/l significantly upregulated the expression of LcPIP1;4,LcPIP1;4a,and LcRAP2.4.Combined treatment with ETH and ABA exerted a stronger inhibitory effect on the bud break and upregulated LcPIP1;4 and LcRAP2.4 to lower degrees than ABA alone,suggesting that ABA reversed the inhibitory effect of ETH on the expression of LcPIP1;4 and LcRAP2.4.ABA treatment and combined treatment with ETH and ABA effectively reduced the moisture content of the terminal buds.These results demonstrate that LcRAP2.4,LcPIP1;4,and LcPIP1;4a play a vital role in dormancy onset of litchi terminal buds by regulating moisture levels. 展开更多
关键词 dormant terminal buds dormancy aquaporin proteins aquaporins lcpip litchi
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Comparative phosphoproteomics analysis of terminal buds at different development stages suggests a pivotal role for protein phosphorylate modification in dormancy regulation of litchi 认领 引用
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作者 Xue Tian Meng-Meng Ma +3 位作者 Hui-Cong Wang Ji-Yuan Shen Ren-Fang Zeng Xu-Ming Huang 《Tropical Plants》 CSCD 2025年第1期167-180,共14页
Protein phosphorylation plays a crucial role in regulating numerous vital cellular and physiological functions in plants.Nevertheless,the molecular mechanisms that control bud dormancy in evergreen fruit trees are not... Protein phosphorylation plays a crucial role in regulating numerous vital cellular and physiological functions in plants.Nevertheless,the molecular mechanisms that control bud dormancy in evergreen fruit trees are not well understood,particularly concerning protein post-translational modifications.In this study,a thorough quantitative phosphoproteomic analysis was performed for litchi terminal buds at different stages to explore the protein phosphorylation dynamics throughout bud development.A total of 6,785 phosphorylation modification sites were identified spanning 2,795 phosphoproteins.Additionally,492 differentially regulated phosphoproteins(DRPs)(253 upregulated and 239 downregulated)were found in the comparison of bud break stage(S2)vs dormancy stage(S1).Two-hundred and eighty four DRPs(147 upregulated and 137 downregulated)were found in the fast growth stage(S3)vs dormancy stage(S1).The comparison between the bud break stage(S2)and the growth cessation stage(S4)(S2 vs S4)revealed 374 DRPs(183 upregulated and 191 downregulated),while in S3 vs S4,186 DRPs(87 upregulated and 99 downregulated)were identified.Gene ontology analysis indicated that most of the DRPs were involved in the protein phosphorylation process,while KEGG pathway analysis revealed that the DRPs were predominantly enriched in metabolism and genetic information processing pathways.Based on the expression patterns,the DRPs at various stages could be categorized into six clusters.An intersection analysis of proteins associated with different functional pathways further highlighted the significant regulatory role of protein kinases in the dormancy removal of litchi terminal buds.Further analysis indicated that the modification levels and expression abundances of DRPs,including CDPK,MAPK,MYB,and zinc proteins,were significantly higher during the growth periods(S2 and S3)compared to the bud growth cessation and dormancy periods(S1 and S4).qRT-PCR results demonstrated that the genes encoding the randomly selected DRPs exhibited notable differential expression patterns across different stages of litchi terminal buds,and some gene expression levels were consistent with the expression abundance levels of their encoded DRPs.This study provides a comprehensive characterization of bud dormancy regulation during the development of shoot terminals in litchi,laying a foundation for further investigation into the regulatory mechanisms of bud dormancy in tropical fruit trees. 展开更多
关键词 phosphoproteomics dormancy regulation litchi terminal buds quantitative phosphoproteomic analysis protein phosphorylation bud dormancy litchi cellular physiological functions
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Spatiotemporally transcriptomic analyses of floral buds reveal the high-resolution landscape of flower development and dormancy regulation in peach 认领 引用 被引量：1
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作者 Ya-lin Zhao Yong Li +7 位作者 Dan-dan Guo Xue-jia Chen Ke Cao Jin-long Wu Wei-chao Fang Chang-wen Chen Xin-wei Wang Li-rong Wang 《Horticulture Research》 SCIE CSCD 2025年第5期162-176,共15页
The spatiotemporal transcriptome dataset reported here provides the peach flower bud’s gene expression atlas at spatiotemporal resolution level using the 10x Genomics Visium platform.This dataset can be used to defin... The spatiotemporal transcriptome dataset reported here provides the peach flower bud’s gene expression atlas at spatiotemporal resolution level using the 10x Genomics Visium platform.This dataset can be used to define transcript accumulation for any interesting genes across several flower bud cells.It was generated using three peach flower bud samples during the activity-dormancy period,providing valuable insight into gene expression profiling and developmental stages under different environmental contexts or conditions.Importantly,we found that different cell types are related to specific regulatory programs,including signal transduction,environment and stress responses,and flower development.Our research provides insight into the transcriptomic landscape of the key cell types for flower buds and opens new avenues to study cell-type specification,function,and differentiation in Rosaceae fruit trees.A series of pivotal genes(e.g.AMS,MS188,MS1)for flower bud development were identified.These results provide a valuable reference for the activity-dormancy transition in perennial deciduous fruit trees. 展开更多
关键词 peach flower bud s developmental stages gene expression atlas gene expression profiling x genomics visium platformthis peach flower bud samples spatiotemporally transcriptomic analyses define transcript accumulation
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Identification of potential metabolites responsible for cold tolerance in buds of Vitis amurensis 认领 引用 被引量：1
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作者 Qingyun Li Huimin Zhou +8 位作者 Fengmei Chai Zemin Wang Linchuan Fang Wei Duan Peige Fan Zhenchang Liang Shaohua Li Qingfeng Wang Haiping Xin 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第1期175-188,共14页
Cold tolerance is one of the important traits for grapevine,especially in regions with extremely low temperatures in winter.Vitis amurensis is wild species in the Vitis genus with excellent cold hardiness compared wit... Cold tolerance is one of the important traits for grapevine,especially in regions with extremely low temperatures in winter.Vitis amurensis is wild species in the Vitis genus with excellent cold hardiness compared with Vitis vinifera.However,metabolites that contribute to the cold tolerance of V.amurensis remain unknown.Here,the metabolomics of buds from V.amurensis‘Zuoshan-1'during cold acclimation(CA)were identified,and cold-sensitive cultivar(V.vinifera‘Jingzaojing')was used as the control.The buds were collected in October,November,and December in 2016 and 2018.The cold hardiness of the buds increased during CA in the two grapevines.However,browning was observed only in V.vinifera buds at temperature below-10℃.Among detected metabolites from buds,443 metabolites were overlapped between two years.Forty-four and thirty differentially accumulated metabolites(DAMs)were identified in V.amurensis and V.vinifera,respectively.Ten DAMs including monoacylglycerol(MAG,18:2)isomer 1,trehalose 6-phosphate,and D-glucose showed identical variations in the two grapevines,indicating conserved CA responses within the Vitis genus.Eighteen DAMs exhibited higher accumulation in V.amurensis than in V.vinifera.Maltotetraose,D-glucoronic acid,L-aspartic acid,azelaic acid,and 4-hydroxybenzoic acid were reported to accumulate during CA in other plants.Enhanced cold tolerance was detected in grapevine leaves with exogenous 5 mmol L-1L-aspartic acid and 1%proanthocyanidins.Potential contributions of other DAMs found in V.amurensis such as Cyanidin 3-O-glucoside need to be further elucidated.Thus,eighteen metabolites accumulated in V.amurensis can be used for practical application in improvement of cold resistance in grapevine.Our findings provide new insights into understanding the cold hardiness of V.amurensis. 展开更多
关键词 Grapevine Metabolomics Bud Low temperature Cold hardiness
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Integration of metabolome and transcriptome reveals flavonoid metabolic network during the developmental stage of flower buds in Cornus wilsoniana 认领 引用
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作者 Jiaqi Cai Haoyu Chao +1 位作者 Ming Chen Dijun Chen 《Medicinal Plant Biology》 2025年第1期276-288,共13页
Secondary metabolites(SMs)are crucial for plant adaptation and human health,yet the regulatory mechanisms underlying their biosynthesis remain incompletely understood.Cornus wilsoniana,a woody oil crop and medicinal p... Secondary metabolites(SMs)are crucial for plant adaptation and human health,yet the regulatory mechanisms underlying their biosynthesis remain incompletely understood.Cornus wilsoniana,a woody oil crop and medicinal plant,is renowned for its rich flavonoid content,yet the genetic and metabolic basis of its secondary metabolism remains largely unexplored.Here,we integrate transcriptomic and metabolomic analyses to dissect the regulatory landscape governing flavonoid biosynthesis during flower bud development.Comparative analyses between high-yield and low-yield genotypes across distinct developmental stages reveal a coordinated reprogramming of flavonoid pathways,with significant shifts in gene expression and metabolite accumulation.WGCNA links critical biosynthetic genes to co-expression modules enriched in hormone signaling,redox homeostasis,and light perception,underscoring the interplay between developmental and environmental cues in shaping metabolic fluxes.Furthermore,comparative genomics reveals 17 candidate genes forming a complete anthocyanin and proanthocyanidin biosynthetic network.Our findings illuminate the molecular framework underlying flavonoid metabolism in C.wilsoniana,providing a foundation for metabolic engineering and genetic improvement strategies aimed at enhancing bioactive compound production for medicinal and agricultural applications. 展开更多
关键词 transcriptome woody oil crop secondary metabolism flavonoid metabolic network secondary metabolites sms transcriptomic metabolomic analyses metabolome flower bud development
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Chemical constituents of the flower buds of Tussilago farfara 认领 引用 被引量：16
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作者 刘玉峰 杨秀伟 武滨 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第4期288-293,共6页
Aim To study the chemical constituents of the flower buds of Tussilago farfara L. in the China National GAP Base of Traditional Chinese Materia Medica and provide scientific basis for quality control. Methods The cons... Aim To study the chemical constituents of the flower buds of Tussilago farfara L. in the China National GAP Base of Traditional Chinese Materia Medica and provide scientific basis for quality control. Methods The constituents were separated and purified by different chromatographic methods, and their structures were elucidated by IR, MS and NMR techniques. Results Twenty eight compounds were isolated from the flower buds of T. farfara. Their structures were identified as n- heptacosane （1）, bis（2-ethylhexyl）phthalate （2）, 7β-[3＇-ethylcrotonoyloxy]-1α-[2＇-methylbutyryloxy]-3,14-dehydro-Z-notonipetranone （3）, 7β-[3＇-ethylcrotonoyloxy]-1α-[2＇-methylbutyryloxy]-3,14-dehydro-E-notonipetranone （4）, tussilagone （5）, dibutyl phthalate （6）, bauer-7-ene-3β,16α-diol （7）, isobauerenol （8）, stigmasterol （9）, β-sitosterol （10）, 2,2-dimethyl-6-acetylchromanone （11）, n- hexadecanoic acid （12）, 7β-hydroxysitosterol （13）, 7α-hydroxysitosterol （14）, 7,14-bisdesacylnotonipetrone （15）, 2,3- dihydroxypropylpalmitate （16）, daucosterol （17）, 6-hydroxy-2,6-dimethylhept-2-en-4-one （18）, ferulic acid （19）, isoferulic acid （20）, caffeic acid （21）, α-D-glucose （22）, sucrose （23）, phthalic acid （24）, p-hydroxybenzoic acid （25）, gallic acid （26）, uridine （27）, and adenosine （28）. Conclusion Compounds 1, 12-16, 18 and 20 were obtained from the genus Tussilago for the first time. 展开更多
关键词 Compositae Flower buds of Tussilago farfara L. Chemical constituents
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Respiratory Response of Dormant Nectarine Floral Buds on Chilling Deficiency 认领 引用 被引量：7
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作者 TAN Yue GAO Dong-sheng +2 位作者 LI Ling CHEN Xiu-de XU Ai-hong 《Agricultural Sciences in China》 2010年第10期1448-1454,共7页
Changes in main biochemical respiratory pathways in dormant nectarine floral buds were studied with nectarine trees(Prunus persica.var,nectariana cv.Shuguang)in order to determine the function of respiration in dorman... Changes in main biochemical respiratory pathways in dormant nectarine floral buds were studied with nectarine trees(Prunus persica.var,nectariana cv.Shuguang)in order to determine the function of respiration in dormancy release.Oxygen-electrode system and respiratory inhibitors were used to measure total respiratory rates and rates of respiratory pathways.Results showed that chilling deficiency blocked the transition of respiratory mode,and made buds stay in a state of high level pentose phosphate pathway(PPP)and low level tricarboxylie acid cycle(TCA).The decline of PPP and activation of TCA occurred synchronously with the release of dormancy.In addition,the inhibition of PPP stimulated a respiration increase related with TCA.It could be concluded that the function of PPP activation in dormancy release might be limited and PPP declination inducing TCA activation might be part of respiration mode transition mechanism during bud sprouting. 展开更多
关键词 nectarine floral buds dormancy release chilling deficiency pentose phosphate pathway tricarboxylic acidcycle
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Respiratory Response of Dormant Nectarine Vegetative Buds to High Temperature Stress 认领 引用 被引量：2
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作者 TAN Yue LI Ling +3 位作者 LENG Chuan-yuan LI Dong-mei CHEN Xiu-de GAO Dong-sheng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第1期80-86,共7页
High temperature stress （HT） is efficient in breaking endo-dormancy of perennial trees. The effects of HT （50°C） on the respiration of dormant nectarine （Prunus persica var. nectariana cv. Shuguang） vegetat... High temperature stress （HT） is efficient in breaking endo-dormancy of perennial trees. The effects of HT （50°C） on the respiration of dormant nectarine （Prunus persica var. nectariana cv. Shuguang） vegetative buds were evaluated in the research. We found that bud respiration was transiently inhibited by HT and the pentose phosphate pathway （PPP） and the cytochrome C pathway （CYT） were significantly affected. On the substrate level, PPP was activated in the HT-treated buds compared with the control group. However, the activation did mot occur until hours after HT treatment. The tricarboxylic acid cycle （TCA） in both the HT-treated buds and in the control group proceeded at a low level most of the time compared with total respiration. On the electron transfer level, CYT was transiently inhibited by HT but became significantly active in the later stage. CYT operation in the control group exhibited an attenuation process. The alternative pathway （ALT） fluctuated both in the HT-treated samples and in the control. The results suggest that the temporary CYT inhibition and the following PPP activation may be involved in HT-induced bud dormancy release and budburst mechanisms. 展开更多
关键词 high temperature stress dormancy release respiration nectarine vegetative buds
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Effect of Photoperiod on Key Enzyme Activities of Respiration in Nectarine Buds During Dormancy Induction 认领 引用 被引量：2
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作者 LI Dong-mei LI Ling +4 位作者 TAN Yue CHEN Xiu-de ZHANG Hai-sen GAO Dong-sheng LI Jin 《Agricultural Sciences in China》 CSCD 2011年第7期1026-1031,共6页
Shuguang （Prunus persica var. nectariana cv. Shuguang） nectarine was used to study effects of photoperiod on key-enzyme activities of respiration during dormancy induction. The dormancy status was determined with sp... Shuguang （Prunus persica var. nectariana cv. Shuguang） nectarine was used to study effects of photoperiod on key-enzyme activities of respiration during dormancy induction. The dormancy status was determined with sprouting ability. Spectrophotometry was used to investigate activities of phosphohexose isomerase （PGI）, malic dehydrogenase （MDH）, and glucose-6-phosphate dehydrogenase （G6PDH）. The results revealed that short day （SD） treatment promoted dormancy induction while long day （LD） treatment postponed the process. During dormancy induction, PGI activities declined, MDH activities changed little, and G6PDH activities increased both in flower buds and leaf buds. PGI activities and MDH activities in SD treatment were lower than control, and G6PDH activities were higher, which was opposite with LD treatment. The changes of respiratory key-enzyme activities were adjusted by photoperiod and correlated with the development of dormancy induction. 展开更多
关键词 photoperiod nectarine buds dormancy induction PGI MDA G6PDH
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Effects of Photoperiod on Alternative Respiration Pathway in Nectarine Flower Buds During Dormancy Induction 认领 引用 被引量：2
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作者 LI Dong-mei TAN Yue +4 位作者 YU Qin CHEN Xiu-de LI Ling ZHANG Hai-sen GAO Dong-sheng 《Agricultural Sciences in China》 CSCD 2011年第12期1881-1886,共6页
Characteristics of dormancy induction and alternative respiration pathway （also known as cyanide-resistant respiration） of nectarine flower buds in different photoperiods were studied to determine the function of ph... Characteristics of dormancy induction and alternative respiration pathway （also known as cyanide-resistant respiration） of nectarine flower buds in different photoperiods were studied to determine the function of photoperiod and alternative respiration pathway in dormancy induction. Oxygen-electrode system and respiratory inhibitors were used to measure total respiratory rates and rates of alternative respiration pathway. The results showed that total respiration rate （Vt） in flower buds showed to be double hump-shaped curves. Short day raised, brought the first-hump of Vt forward and delayed the second-hump, while long day delayed the whole curve. The capacity （Vast） and activity （pValt） of SD and LD changed synchronously and both showed to be double hump-shaped curves. Short day made the first climax of Vast and pValt existed much earlier, while long day increased their rates significantly. The length of day had little effects on the later period climax. Long day also increased the contributions of altemative respiration pathway in total respiration rate （pValt/Vt）. The changes in alternative respiration pathway were correlated with the induction of dormancy and adjusted by photoperiod. Short day promoted dormancy induction of nectarine trees, while long day delayed it. 展开更多
关键词 photoperiod nectarine flower buds dormancy induction alternative respiration pathway
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Relation Between Endodormancy Induction and Changes in Two Main Electron Transport Pathways of Nectarine Buds 认领 引用 被引量：1
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作者 YU Qin GAO Dong-sheng XU Xiao-ming LI Jin XU Chen-shan 《Agricultural Sciences in China》 2008年第10期1203-1209,共7页
Operation regulations of two main electron transport pathways in nectarine （Prunus persica var. nectariana cv. Shuguang） buds during endodormancy induction were studied to understand possible roles which two main el... Operation regulations of two main electron transport pathways in nectarine （Prunus persica var. nectariana cv. Shuguang） buds during endodormancy induction were studied to understand possible roles which two main electron transport pathways played in the buds of deciduous fruit trees during endodormancy induction. Respiratory inhibitors （KCN and SHAM） were used to investigate total respiration rate （Vt）, the development and operation of the alternative pathway and partitioning of electrons between the cytochrome and alternative pathways in nectarine buds during endodormancy induction. Results indicated that changes of Vt in flower and leaf buds showed single and double hump-shaped curves, respectively. In endodormancy induction, the capacity （Valt,） and activity （ρValt） of the alternative pathway rapidly increased, but changes of them had different patterns during the entire measuration. At the same time, changes of engagements of the alternative （ρValt/Vt） and cytochrome pathway （ρ′Vcyt/Vt） were opposite, and ρ′Vcyt/Vt was always further higher than ρValt/Vt during the entire measuration. All these results indicated that the development and operation of the alternative pathway played important roles in endodormancy induction, but the cytochrome pathway was the main pathway for mitochondrial electron transport in buds during endodormancy induction. 展开更多
关键词 nectarine buds endodormancy induction cytochrome pathway alternative pathway
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MIKC type MADS-box transcription factor LcSVP2 is involved in dormancy regulation of the terminal buds in evergreen perennial litchi(Litchi chinensis Sonn.) 认领 引用 被引量：4
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作者 Meng-Meng Ma Hui-Fen Zhang +5 位作者 Qi Tian Hui-Cong Wang Fang-Yi Zhang Xue Tian Ren-Fang Zeng Xu-Ming Huang 《Horticulture Research》 SCIE CSCD 2024年第7期294-306,共13页
SHORT VEGETATIVE PHASE(SVP),a member of the MADS-box transcription factor family,has been reported to regulate bud dormancy in deciduous perennial plants.Previously,three LcSVPs(LcSVP1,LcSVP2 and LcSVP3)were identifie... SHORT VEGETATIVE PHASE(SVP),a member of the MADS-box transcription factor family,has been reported to regulate bud dormancy in deciduous perennial plants.Previously,three LcSVPs(LcSVP1,LcSVP2 and LcSVP3)were identified from litchi genome,and LcSVP2 was highly expressed in the terminal buds of litchi during growth cessation or dormancy stages and down-regulated during growth stages.In this study,the role of LcSVP2 in governing litchi bud dormancy was examined.LcSVP2 was highly expressed in the shoots,especially in the terminal buds at growth cessation stage,whereas low expression was showed in roots,female flowers and seeds.LcSVP2 was found to be located in the nucleus and have transcription inhibitory activity.Overexpression of LcSVP2 in Arabidopsis thaliana resulted in a later flowering phenotype compared to the wild-type control.Silencing LcSVP2 in growing litchi terminal buds delayed re-entry of dormancy,resulting in significantly lower dormancy rate.The treatment also significantly up-regulated litchi FLOWERING LOCUS T2(LcFT2).Further study indicates that LcSVP2 interacts with an AP2-type transcription factor,SMALL ORGAN SIZE1(LcSMOS1).Silencing LcSMOS1 promoted budbreak and delayed bud dormancy.Abscisic acid(200 mg/L),which enforced bud dormancy,induced a short-term increase in the expression of LcSVP2 and LcSMOS1.Our study reveals that LcSVP2 may play a crucial role,likely together with LcSMOS1,in dormancy onset of the terminal bud and may also serve as a flowering repressor in evergreen perennial litchi. 展开更多
关键词 regulate bud dormancy deciduous perennial plantspreviouslythree short vegetative phase svp litchi bud dormancy lcsvp growth cessation mads box transcription factor terminal buds
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In comparison with vitamin C and butylated hydroxytoluene, the antioxidant capacity of aqueous extracts from buds and flowers of Lonicera japonica Thunb. 认领 引用 被引量：5
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作者 Zhou Yunfeng Li Lin +2 位作者 Sun Lan Zhou Lidong Xu Yang 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2018年第3期373-379,共7页
OBJECTIVE: To assess the antioxidant capacity of aqueous extracts of buds and flowers of Lonicera japonica Thunb.(BLJ and FLJ) using in vitro assays.METHODS: We assessed the in vitro antioxidant activities of aqueous ... OBJECTIVE: To assess the antioxidant capacity of aqueous extracts of buds and flowers of Lonicera japonica Thunb.(BLJ and FLJ) using in vitro assays.METHODS: We assessed the in vitro antioxidant activities of aqueous extracts of BLJ and FLJ and compared with that of classical antioxidants vitamin C and butylated hydroxytoluene, using several well-established methods including the 1,1-diphenyl-2-picryl-hydrazyl assay, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay, reducing power assay, fluorescence recovery after photobleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.RESULTS: The aqueous extracts of both BLJ and FLJ had similarly potent antioxidant capacity. There were no significance differences between BLJ and FLJ in all the assays.CONCLUSION: The aqueous extracts of both BLJ and FLJ have antioxidant activity with comparable efficacy. These findings suggest that both BLJ and FLJ may have the potential as natural antioxidants. 展开更多
关键词 Buds Flowers Lonicera Japonica Freeradical scavengers Lipid peroxidation
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An efficient method for clonal propagation and in vitro establishment of softwood shoots from epicormic buds of teak(Tectona grandis L.) 认领 引用 被引量：5
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作者 Muhammad AKRAM Faheem AFTAB 《Forestry Studies in China》 2009年第2期105-110,共6页
Softwood shoots were produced from 40 cm long stem segments placed horizontally in flat trays containing sterilized sand under natural light or shade conditions for subsequent rooting and micropropagation studies in t... Softwood shoots were produced from 40 cm long stem segments placed horizontally in flat trays containing sterilized sand under natural light or shade conditions for subsequent rooting and micropropagation studies in teak （Tectona grand＆ L.）. Higher number of shoots （6.17） per log was produced under natural light as compared to shade conditions. Forcing was also better in natural light as compared to shade in terms of shoot length, number of nodes or leaves. For rooting, 2-4 cm long softwood shoots were excised and treated with either indole-3-butyric acid （IBA） or α-naphthyl acetic acid （NAA） at 0, 1000, 2000 or 3000 μmol.L^-1 each or with combinations （1000 ＋ 1000, 2000 ＋ 2000 or 3000 ＋ 3000 μmol.L^-1） and then placed in flat trays containing autoclaved sand at 25 ± 2℃ in 16 h photoperiod at 35 μmol.m^-2.s^-1. After 28 days, softwood cuttings treated with IBA ＋ NAA （3000 ＋ 3000 μmol.L^-1） had highest rooting percentage （89.3%） with 5.5 mean roots. Shoot apex and nodal explants of softwood cuttings were pretreated with 0.1% （w/v） ascorbic acid, boric acid, activated charcoal, citric acid, glutamine or polyvinylpolypyrollidone （PVP） for 24 h to remove phenolic compounds before surface disinfestation. Glutamine （G1） and PVP were equally effective resulting in 60% establishment of shoot apices on MS medium supplemented with 10 μmol.L^-1 6-benzylaminopurine （BAP） ＋ 5 μmol.L^-1 NAA. Using shoot apices, highest （42.80） number of multiple shoots with 54.33 mm shoot length were obtained on MS ＋ BAP （8.8 p.mol.L 1） ＋ IBA （2 μmol.L^-1） after 45 days. Shoots were successfully rooted and acclimatized to greenhouse conditions. 展开更多
关键词 epicormic buds glutamine PVP phenolics Tectona grandis L. tissue culture softwood shoots
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Effect of auxins on axillary and de novo shoot regeneration from in vitro shoot cultures derived from forced epicormic buds of teak (Tectona grandis L.) 认领 引用 被引量：2
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作者 Akram MUHAMMAD Aftab FAHEEM 《Forestry Studies in China》 2012年第3期180-186,共7页
Akram MUHAMMAD, Aftab FAHEEM＊Abstract In this presentation, we report on de novo and axillary shoot regeneration and rooting of shoots maintained over a long term, from cultures of Tectona grandis L. Shoot-tips of te... Akram MUHAMMAD, Aftab FAHEEM＊Abstract In this presentation, we report on de novo and axillary shoot regeneration and rooting of shoots maintained over a long term, from cultures of Tectona grandis L. Shoot-tips of teak shoots forced from epicormic buds were used as the starting material for axenie shoot-culture establishment. Long term maintenance of such axenic shoot cultures was carried out by regular sub-culturing on MS media supplemented with N6-benzyleadenine （BA, 8.8 μmol·L^-1） and indole-3-butyric acid （IBA, 2 μmol·L ^1） for 24 months. Vigorously growing shoot tips （2-3 cm long） were inoculated on the MS basal medium supplemented with different concentrations （0, 1, 2, 4, 6, 8 or 10 p.mol-L-~） of either [BA or a-naphthaleneacetic acid （NAA） for rooting. Axillary and de novo shoots were de- veloped from axillary and cut basal ends of shoots, respectively. Shoots growing on auxins were further sub-cultured （every 15 days） and maintained for 45 days. The greatest number of de novo （5.06） as well as axillary shoots （2.85） was observed on the MS medium supplemented with 10 μmol-L^-1 NAA or 8 μmol·L^-1 IBA, respectively, after 45 days. The combinations of both IBA （μmol·L^-1） ＋ NAA （μmol·L^-1） were tested at different concentrations （4 ＋ 4, 6 ＋ 6, 8 ＋ 8） supplemented to a half strength MS basal medium with 0.1% activated charcoal for rooting of decapitated and non-decapitated de novo and axillary shoots. Rooting from non-decapitated de novo shoots was highest （93.33%） with a mean number of roots of 4.61 on this medium, supplemented with 6 μmol·L^-1 IBA ＋ 6 gmol.L l NAA, after 36 days of initial culture. Individual auxin, however, was not effective for root induction. Rooted shoots were acclimatized in a green house and after four weeks plantlets were transferred to the field. 展开更多
关键词 auxins de novo shoots epicormic buds in vitro teak
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Effects of key growth conditions on endogenous hormone content in tillering stem bases,germination of lateral buds,and biomass allocation in Indocalamus decorus 认领 引用 被引量：3
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作者 Guibin Gao Hao Zhong +4 位作者 Zhizhuang Wu Neng Li Zheke Zhong Yanhong Pan Liangru Wu 《Journal of Forestry Research》 SCIE CAS CSCD 2018年第6期1547-1555,共9页
Physiological responses and changes in growth of Indocalamus decorus Q.H.Dai under different ecological conditions are essential for further understanding growth regulation and adaptive mechanisms and establishing an ... Physiological responses and changes in growth of Indocalamus decorus Q.H.Dai under different ecological conditions are essential for further understanding growth regulation and adaptive mechanisms and establishing an evidence-based management system for optimal growth. In this study, the endogenous hormone content in tillering stem bases, germination of lateral buds, and biomass allocation of this bamboo species in different growth environments were investigated. Among the endogenous hormones in the basal stems of tillers, indole-3-pyruvic acid and zeatin riboside were highly correlated with lateral buds that germinated to form shoots, while gibberellic acid was highly correlated with lateral buds that germinated to form rhizomes. The best lateral bud germination characteristics were achieved with full sun, a density of six plantlets per pot, and watering every 6 days. I. decorus plantlets used different resource allocation strategies depending on treatment. Different ecological factors influenced endogenous hormones in the bamboo stem base,which affected lateral bud germination and biomass allocation. 展开更多
关键词 Biomass allocation Ecological factor Indocalamus decorus Lateral bud Plantlet Tillering
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GC-MS Analysis of Liposoluble Constituents from Buds,Flowers and Fruits of Millettia speciosa Champ. 认领 引用 被引量：1
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作者 Maoyuan WANG Qing YANG +3 位作者 Qinglong WANG Xiaoxia YAN Jianrong WANG Zhunian WANG 《Medicinal Plant》 2017年第4期10-14,共5页
[Objectives] To study the material foundation of liposoluble constituents from buds,flowers and fruits of Millettia speciosa Champ.and provide a reference for the development of flowers and fruits of Millettia specios... [Objectives] To study the material foundation of liposoluble constituents from buds,flowers and fruits of Millettia speciosa Champ.and provide a reference for the development of flowers and fruits of Millettia speciosa Champ.[Methods]The liposoluble constituents were extracted from buds,flowers and fruits of Millettia speciosa by Soxhlet extraction and solvent extraction method,and analyzed by GC-MS.[Results]24 compounds were identified from the liposoluble constituents of buds,accounting for 88. 31 % of the total liposoluble constituents,mainly comprising alkanes and olefins compounds( 52. 00%) and alcohols compounds( 17. 46%); 29 compounds were identified from the liposoluble constituents of flowers,accounting for 91. 38 % of the total liposoluble constituents,mainly comprising alkanes and olefins compounds( 60. 64%) and alcohols compounds( 17. 17%); 32 compounds were identified from the liposoluble constituents of fruits,accounting for 80. 01 % of the total liposoluble constituents,mainly comprising alkanes and olefins compounds( 32. 56%),phenyl and its derivatives compounds( 22. 46%) and fatty acids compounds( 12. 54%). 6 compounds were common in buds,flowers and fruits. [Conclusions] Although there were some differences in liposoluble constituents from flowers,fruits,leaves and roots of Millettia speciosa Champ.,the different parts of Millettia speciosa Champ. had development value. 展开更多
关键词 Millettia speciosa Champ Buds Flowers Fruits GC-MS Liposoluble constituents
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Effect of Low Temperature Treatment on Agrobacterium-mediated Transformation of Maize Seed Buds 认领 引用 被引量：1
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作者 Mengyu LYU Fushuang DONG +6 位作者 Junmin ZHANG He ZHAO Jianfang CHAI Shuo ZHOU Fan YANG Yongwei LIU Haibo WANG 《Agricultural Biotechnology》 2018年第3期75-76,共2页
[Objectives]This study was conducted to improve the genetic transformation rate of maize. [Methods]The seeds of maize ＂ Zheng 58＂ as the experimental material were germinated and treated under freezing temperature o... [Objectives]This study was conducted to improve the genetic transformation rate of maize. [Methods]The seeds of maize ＂ Zheng 58＂ as the experimental material were germinated and treated under freezing temperature of-18 ℃ for 30 and 35 min. The EGFP gene was transformed into the growth points of these seed buds by Agrobacterium tumefaciens EHA105. The transformation effect was determined by fluorescence protein detection on transformed buds. [Results]After a certain period of treatment at-18 ℃ following germination and transformation of maize bud growth points with A. tumefaciens,although the survival rate of maize buds was reduced,the percentage of transformed cells significantly increased. [Conclusions]Low temperature treatment can improve the transformation efficiency of A. tumefaciens to the growth point of maize bud. 展开更多
关键词 Maize buds Low temperature treatment Agrobacterium Transgenic
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Preliminary Study on Induction and Identification of Polyploidy of Shandong Salvia miltiorrhiza Buds Treated by Colchicine 认领 引用 被引量：1
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作者 房翠萍 单成钢 +3 位作者 王维婷 陈庆亮 倪大鹏 王志芬 《Agricultural Science & Technology》 CAS 2011年第9期1338-1341,共4页
[Objective] The aim was to study the feasibility of using colchicine to induce polyploidy from the buds of Shandong Salvia miltiorrhiza.[Method] The buds of Shandong Salvia miltiorrhiza were induced by colchicine.[Res... [Objective] The aim was to study the feasibility of using colchicine to induce polyploidy from the buds of Shandong Salvia miltiorrhiza.[Method] The buds of Shandong Salvia miltiorrhiza were induced by colchicine.[Result] When the concentration of colchicine supplemented in culture medium was 12 mg/L,the induction rate of polyploidy was the highest（36.6%）,but its survival rate was only 16.7%.When the concentration of colchicine was 8 mg/L,the induction rate of polyploidy was 15.9%,and its survival rate was up to 40.0%.Polyploidy plant of Shandong Salvia miltiorrhiza was strong and had thick,large and dark leaves,and long stomata on the lower epidermis of leaves had small density.[Conclusion] Colchicine could induce buds effectively,and the polyploidy breeding material of Salvia miltiorrhiza was obtained,which laid a foundation for shortening breeding process. 展开更多
关键词 Coichicine Shandong Salvia miltiorrhiza Multiple bud Polyploidy Stomatal identification
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Optimum harvesting time of herbaceous peony buds for cutting flowers 认领 引用 被引量：2
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作者 YU Xiao-nan GUO Peng-peng LU Guang-pei ZHANG Qi-xiang 《Journal of Forestry Research》 CAS CSCD 2011年第1期137-140,共4页
The herbaceous peony is one of the cut flowers that has become increasingly popular in the international market in recent years. In the study, 11 cultivars of herbaceous peonies suitable for cutting flowers were selec... The herbaceous peony is one of the cut flowers that has become increasingly popular in the international market in recent years. In the study, 11 cultivars of herbaceous peonies suitable for cutting flowers were selected; different harvesting stages （three or four stages） were identified according to bud development observation （bud firmness, bud diameter, sepal angle, petal angle, and color showing）. Moreover, flower development and vase life were also recorded in the experiment of vase. Bud development observation shows that there are great differences in optimum harvesting stage between the culti- vars, especially between the hybrid peony group and lactiflora group. This implies that bud diameter could not be the only indicator for optimum harvesting stage. Bud description, including sepal opening angle, sepal color, petal color showing, and degree of firmness, is necessary for the determination of the harvesting stage. Among the 11 cultivars,‘Pink Hawaiian Coral＇, ‘Red Charm＇, ‘Edulis Superba＇, ‘Red Magic＇, and ‘Sarah Bernhardt＇ should be harvested at stage 1. ‘Duchesse de Nemours＇, ‘Taff＇, ‘Sorbet＇ and ‘Monsieur Jules Elie＇ should be harvested at stage 2. ‘Kansas＇ should be at stage 3 and ‘Karl Rosenfield＇ at stage 4. The determination of optimum harvesting stage for each cultivar is an important element in cut flower production. 展开更多
关键词 herbaceous peony cut flower harvesting stage bud maturity
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