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Pulmonary hypertension causing uveal effusion syndrome: a case report 认领 引用
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作者 Qian Ma Hong-Lei Liu Wei-Wei Wang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2025年第10期2007-2010,共4页
I am writing this letter to present a case of uveal effusion syndrome(UES)caused by pulmonary hypertension found due to intermittent high intraocular pressure(IOP).Pulmonary hypertension increases cardiac afterload du... I am writing this letter to present a case of uveal effusion syndrome(UES)caused by pulmonary hypertension found due to intermittent high intraocular pressure(IOP).Pulmonary hypertension increases cardiac afterload due to elevated pulmonary vascular resistance,leading to right heart failure and death[1].Notably,ocular complications of pulmonary hypertension have been reported less frequently[2-5].Ophthalmologists need to increase the understanding of the disease so as to perform the proper treatment for these patients.Ethical Approval The study was conducted in accordance with the principles of the Declaration of Helsinki.Written informed consent was obtained from the patient for publication of the images and data included in this article. 展开更多
关键词 Right Heart Failure increase understanding disease pulmonary hypertension Intraocular Pressure uveal effusion syndrome ues caused Ocular Complications pulmonary vascular resistanceleading Uveal Effusion Syndrome
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Whole exome sequencing identifies an AMBN missense mutation causing severe autosomal-dominant amelogenesis imperfecta and dentin disorders 认领 引用 被引量：5
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作者 Ting Lu Meiyi Li +9 位作者 Xiangmin Xu Jun Xiong Cheng Huang Xuelian Zhang Aiqin Hu Ling Peng Decheng Cai Leitao Zhang Buling Wu Fu Xiong 《International Journal of Oral Science》 SCIE CAS CSCD 2018年第4期223-231,共9页
Tooth development is a complex process that involves precise and time-dependent orchestration of multiple genetic, molecular,and cellular interactions. Ameloblastin(AMBN, also named "amelin" or "sheathl... Tooth development is a complex process that involves precise and time-dependent orchestration of multiple genetic, molecular,and cellular interactions. Ameloblastin(AMBN, also named "amelin" or "sheathlin") is the second most abundant enamel matrix protein known to have a key role in amelogenesis. Amelogenesis imperfecta(AI [MIM: 104500]) refers to a genetically and phenotypically heterogeneous group of conditions characterized by inherited developmental enamel defects. The hereditary dentin disorders comprise a variety of autosomal-dominant genetic symptoms characterized by abnormal dentin structure affecting either the primary or both the primary and secondary teeth. The vital role of Ambn in amelogenesis has been confirmed experimentally using mouse models. Only two cases have been reported of mutations of AMBN associated with non-syndromic human AI. However, no AMBN missense mutations have been reported to be associated with both human AI and dentin disorders.We recruited one kindred with autosomal-dominant amelogenesis imperfecta(ADAI) and dentinogenesis imperfecta/dysplasia characterized by generalized severe enamel and dentin defects. Whole exome sequencing of the proband identified a novel heterozygous C-T point mutation at nucleotide position 1069 of the AMBN gene, causing a Pro to Ser mutation at the conserved amino acid position 357 of the protein. Exfoliated third molar teeth from the affected family members were found to have enamel and dentin of lower mineral density than control teeth, with thinner and easily fractured enamel, short and thick roots, and pulp obliteration. This study demonstrates, for the first time, that an AMBN missense mutation causes non-syndromic human AI and dentin disorders. 展开更多
关键词 Whole exome sequencing identifies an AMBN missense mutation causing severe autosomal-dominant amelogenesis imperfecta and dentin disorders
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THE CHARACTERISTIC, DISTRIBUTION AND CAUSING FACTORC OF THE DISABLED IN HENAN PROVINCE 认领 引用
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作者 林富瑞 李春发 《Chinese Geographical Science》 SCIE 1995年第1期77-86,共10页
Henan Province is not only one of the provinces with the most POpulation, but also one of the provinces with the most disabled people in China. The characteristics of the disabled People, the distribution law of six t... Henan Province is not only one of the provinces with the most POpulation, but also one of the provinces with the most disabled people in China. The characteristics of the disabled People, the distribution law of six typeS of the disabled, and the reason c 展开更多
关键词 disabled basic characteristic distributive law factor of causing disabled
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LOTUS, a potent blocker of Nogo receptor-1 causing inhibition of axonal growth 认领 引用 被引量：1
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作者 Yuji Kurihara Kohtaro Takei 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第1期46-48,共3页
Glia-derived axonal growth inhibitory proteins limit functional repair following damage to the adult cen- tral nervous system （CNS）. Nogo proteins, myelin-as- sociated glycoprotein （MAG）, oligodendrocyte myelin gl... Glia-derived axonal growth inhibitory proteins limit functional repair following damage to the adult cen- tral nervous system （CNS）. Nogo proteins, myelin-as- sociated glycoprotein （MAG）, oligodendrocyte myelin glycoprotein （OMgp） and B lymphocyte stimulator （BLyS）, are 4 inhibitors that commonly interact with the neuronal receptor, Nogo receptor-1 （NgR1）, lead- ing to inhibition of axonal growth. Here, we demon- strate that lateral olfactory tract usher substance （LOTUS） binds to NgR1 and blocks the binding of all four ligands to NgR1, resulting in the suppression of axonal growth inhibition induced by these NgR1 li- gands. LOTUS allows neurons to overcome NgRl-me- diated axonal growth inhibition, raising the possibility that LOTUS may be useful in future therapeutic ap- proaches as an endogenous potent inhibitor of NgR1 for promoting neuronal regeneration. 展开更多
关键词 OMgp MAG a potent blocker of Nogo receptor-1 causing inhibition of axonal growth LOTUS
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Organisms causing spontaneous bacterial peritonitis in children with liver disease and ascites in Southern Iran 认领 引用 被引量：4
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作者 Mahmood Haghighat Seyed Mohsen Dehghani +3 位作者 Abdolvahab Alborzi Mohammad Hadi Imanieh Bahman Pourabbas Mehdi Kalani 《World Journal of Gastroenterology》 SCIE CAS 2006年第36期5890-5892,共3页
AIM： To determine the causative agents of spontaneous bacterial peritonitis （SBP） in children with liver disease and ascites in our center. METHODS： During a 2.5 year period, from September 2003 to March 2006, 12 ... AIM： To determine the causative agents of spontaneous bacterial peritonitis （SBP） in children with liver disease and ascites in our center. METHODS： During a 2.5 year period, from September 2003 to March 2006, 12 patients with 13 episodes of SBP were studied. In all cases at the time of admission serum albumin and glucose, urinalysis and urine culture was performed. Analysis [white blood cell （WBC） count with differential, albumin, glucose], gram stain, culture by BACTEC method and antibiogram was done on ascitic fluids. Abdominal paracentesis was repeated after 48 h of antibiotic therapy for bacteriologic assay. The patients were followed for at least three months in a gastroenterology clinic. RESULTS： There were 7 girls （58%） and 5 boys （42%） with a median age of 5.2 years （range, 6 mo to 16 years）. All cases had positive ascitic fluid culture. Gram stain was positive in 5 （38.5%） of them. The isolated organisms were S. pneumoniae in 5 （38.5%）, E. coli in 2 （15.3%）, S. viridans in 2 （15.3%）, and K. pneumoniae, H. influenza, Enterococci, and nontypable Streptococcus each in one （7.7%）. All of them except Enterococci were sensitive to ciprofloxacin and ceftriaxone. All ascitic fluid cultures were negative after 48 h of antibiotic therapy. CONCLUSION： S. pneumoniae is the most common cause of SBP in the pediatric age group and we recommend a third generation cephalosporine （e.g., Ceftriaxlone or Cefotaxime） for empirical therapy in children with SBP. 展开更多
关键词 Spontaneous bacterial peritonitis Children Cirrhosis Causes Empirical therapy
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Rapid and Accurate Detection of the Pathogen Causing Tobacco Target Spot Based on Loop-Mediated Isothermal Amplification 认领 引用
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作者 LI Ping YIN Xiu-juan +4 位作者 WANG Ying ZHOU Xiang-ping LIU Tian-bo ZHONG Jie XIAO Yan-song 《Agricultural Science & Technology》 CAS 2024年第4期27-32,共6页
This study established a loop-mediated isothermal amplification(LAMP)system for specially detecting the pathogen causing tobacco target spot in a rapid manner.With genes encoding the Internal Transcribed Spacer(ITS)as... This study established a loop-mediated isothermal amplification(LAMP)system for specially detecting the pathogen causing tobacco target spot in a rapid manner.With genes encoding the Internal Transcribed Spacer(ITS)as targets,four LAMP primers with high specificity and sensitivity were designed and screened.The specificity and sensitivity of the established method were evaluated,and then the method was used to detect the samples of tobacco plants artificially inoculated with pathogens.The established method enabled the observation of detection results by the color change of dye after 60 min of isothermal amplification at 65°C.In the specificity test,only the reaction tubes of the pathogen causing tobacco target spot appeared green,and the electrophoresis lanes were dispersive,indicating positive results,while all other fungal strains showed negative results.The method had a minimum limit of detection being 0.1 ng/μL for the genomic DNA of the pathogen causing tobacco target spot,allowing direct detection of the pathogen from lesions.The established LAMP method can be directly used for the diagnosis of tobacco plants infected with target spot in the field. 展开更多
关键词 Pathogen causing tobacco target spot Detection Loop-mediated isothermal amplification(LAMP)
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In silico analysis of a disease-causing mutation in PCDH15 gene in a consanguineous Pakistani family with Usher phenotype 认领 引用
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作者 Shamim Saleha Muhammad Ajmal +2 位作者 Muhammad Jamil Muhammad Nasir Abdul Hameed 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第5期662-668,共7页
AIM： To map Usher phenotype in a consanguineous Pakistani family and identify disease-associated mutation in a causative gene to establish phenotype-genotype correlation.· METHODS： A consanguineous Pakistani fa... AIM： To map Usher phenotype in a consanguineous Pakistani family and identify disease-associated mutation in a causative gene to establish phenotype-genotype correlation.· METHODS： A consanguineous Pakistani family in which Usher phenotype was segregating as an autosomal recessive trait was ascertained. On the basis of results of clinical investigations of affected members of this family disease was diagnosed as Usher syndrome（USH）. To identify the locus responsible for the Usher phenotype in this family, genomic DNA from blood sample of each individual was genotyped using microsatellite Short Tandem Repeat（STR） markers for the known Usher syndrome loci. Then direct sequencing was performed to find out disease associated mutations in the candidate gene.· RESULTS： By genetic linkage analysis, the USH phenotype of this family was mapped to PCDH15 locus on chromosome 10q21.1. Three different point mutations in exon 11 of PCDH15 were identified and one of them,c.1304AC was found to be segregating with the disease phenotype in Pakistani family with Usher phenotype.This, c.1304 A C transversion mutation predicts an amino-acid substitution of aspartic acid with an alanine at residue number 435（p.D435A） of its protein product.Moreover, in silico analysis revealed conservation of aspartic acid at position 435 and predicated this change as pathogenic.·CONCLUSION：Theidentificationofc.1304ACpathogenic mutation in PCDH15 gene and its association with Usher syndrome in a consanguineous Pakistani family is thefirst example of a missense mutation of PCDH15 causing USH1 phenotype. In previous reports, it was hypothesized that severe mutations such as truncated protein of PCDH15 led to the Usher I phenotype and that missense variants are mainly responsible for non-syndromic hearing impairment. 展开更多
关键词 deafness and blindness Usher syndrome causative gene missense mutation Pakistani family
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Livelihood Economic Activities Causing Deforestation in Northern Ghana: Evidence of Sissala West District 认领 引用 被引量：1
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作者 Clifford James Fagariba Shaoxian Song Serge K. G. Soule 《Open Journal of Ecology》 2018年第1期57-74,共18页
Policymakers and international organizations are making the conscious effort to address climate change through afforestation and sustainable ecosystem management. Economic activities including agriculture, mining, and... Policymakers and international organizations are making the conscious effort to address climate change through afforestation and sustainable ecosystem management. Economic activities including agriculture, mining, and infra-structure improvement to meet basic human needs continuously degrade the natural and forest resources. The rate of deforestation in Ghana is alarming due to over-reliance on forest resources by forest-dependent communities. Perceived causes of deforestation differ from individuals and groups perspec-tive. This depends on factors including environmental knowledge, education level, market demand and socio-economic activities. Simple random sampling and key informant interview with the aid of semi-structured questionnaire was used to elicit information from the indigenes of Sissala West District to determine their perceptions of causes of deforestation. The study indicated that poverty, high illiteracy, population growth and lack of alternative source of livelihood were the indirect causes which trigger livelihood economic activities such as farming, charcoal burning, wood logging and hunting leading to degradation of the ecosystem. It was also realized that majority of the indigenes are uneducated and this contributes to their unawareness of rate of de-forestation. Recommendations suggested to address challenges were enforce-ment of bye-laws and stringent government environmental policies to deter people from degrading the forest. Education, agroforestry, afforestation, and provision of alternative livelihood were also good interventions suggested. 展开更多
关键词 Deforestation Livelihood Activities Underlying Causes Forest Communities
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Discussion on the Prevention and Treatment of COVID-19 Causing Lung Disease and Heart Damage Based on Lei Zhongyi's Theory of Intermingled Phlegm,Blood Stasis and Toxin 认领 引用
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作者 Jinfeng CHEN Zhongyi LEI +6 位作者 Chaofeng LIU Hong FAN Peng LEI Xueping WU Xiaoyong YU Yanfen ZHOU Jiejun HOU 《Medicinal Plant》 2020年第6期6-11,共6页
Novel coronavirus infection not only damages lung function,but also causes myocardial injury,elevated myocardial enzymes and heart failure,especially for patients with basic heart diseases who develop COVID-19,the fir... Novel coronavirus infection not only damages lung function,but also causes myocardial injury,elevated myocardial enzymes and heart failure,especially for patients with basic heart diseases who develop COVID-19,the first consideration should be the protection of cardiac function.Based on the theory of intermingled phlegm,blood stasis and toxin of heart disease put forward by Master Lei Zhongyi,the dialectical treatment thinking of COVID-19 patients from the concept of damage of phlegm,blood stasis and toxin to the heart were discussed.During the diagnosis,critical stage and recovery period of COVID-19,expectorant and blood-activating agents,heat and detoxification agents can be added to promote lung and asthma,free Bizheng and remove blood stasis,calm the heart and calm the mind,and promote the recovery of cardiopulmonary functions. 展开更多
关键词 COVID-19 Intermingled phlegm Blood stasis and toxin Lei Zhongyi Lung disease and heart damage Blood stasis caused by epidemic toxin
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Perception-Based Assessment of the Factors Causing Delays in Construction Projects 认领 引用
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作者 Om Prakash Giri 《Engineering（科研）》 2023年第7期431-445,共15页
Construction delay is a widespread issue in the construction industry of developing countries, and Nepal is no exception. These delays extend project durations and lead to cost overruns and disputes among stakeholders... Construction delay is a widespread issue in the construction industry of developing countries, and Nepal is no exception. These delays extend project durations and lead to cost overruns and disputes among stakeholders. To address this problem, this study aimed to identify and analyze the significant factors that contribute to construction project delays in Nepal. To gather data, a well-structured questionnaire was developed and administered to a sample of 100 participants, including contractors, consultants, and civil engineers. Various statistical tests were conducted to ensure the data’s integrity and consistency, such as reliability assessments and factor analyses. The findings of the study highlighted multiple factors contributing to delays in construction projects such as inadequate design, poor communication, and coordination among stakeholders, insufficient experience and planning by contractors, delays in material delivery and testing, labor-related problems including shortages and low qualifications, and external factors like regulatory changes and unforeseen circumstances. By identifying these major causes of construction project delays, this study presented insightful information that can contribute to the analysis and evaluation of project performance. 展开更多
关键词 Construction Projects Construction Industry Delay Causes Construction De-lays Project Performance
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Staphylococcus aureus Cultured from Sushi in China:Revealing the Potential Foodborne Pathogenic Risks based on Antimicrobial Susceptibility,Virulence Factors and Genetic Characteristics 认领 引用
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作者 Hui Li Xin Gan +6 位作者 Menghang Li Shiwei Liu Dajin Yang Lin Yan Séamus Fanning Li Bai Zhaoping Liu 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2026年第2期239-244,共6页
Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contribu... Staphylococcus aureus(S.aureus)is the third most common pathogen causing 10.6%of bacterial foodborne illnesses in China in 2021[1].Heat-stable Staphylococcal Enterotoxins(SEs)produced by S.aureus are the main contributors to staphylococcal food poisoning(SFP),causing vomiting,diarrhea,abdominal pain,headache,muscle cramps,and other acute gastroenteritis symptoms.More than 25 SEs and staphylococcal enterotoxin-like toxins(SE/s)have been described and which together comprise a superfamily of pyrogenic toxin superantigens(SAgs)[2]. 展开更多
关键词 bacterial foodborne illnesses staphylococcus aureus saureus foodborne pathogenic risks staphylococcal food poisoning sfp causing pyrogenic toxin sup antimicrobial susceptibility genetic characteristics virulence factors
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Negative electricity prices in electricity markets:causes,impacts,and response strategies 认领 引用 被引量：1
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作者 Qingkai Sun Haifeng Zheng +3 位作者 Zheng Zhao Menghua Fan Xiaojun Wang Yiru Shi 《Global Energy Interconnection》 EI CSCD 2026年第3期531-543,共13页
Against the backdrop of the accelerating global clean energy transition and the rapid increase in renewable energy source(RES)penetration,temporal imbalances between supply and demand in power systems are becoming inc... Against the backdrop of the accelerating global clean energy transition and the rapid increase in renewable energy source(RES)penetration,temporal imbalances between supply and demand in power systems are becoming increasingly prominent.Consequently,the phenomenon of negative electricity prices has evolved from sporadic occurrences into an increasingly prevalent market signal.This paper focuses on case studies of negative electricity prices in major international electricity markets(the Netherlands,Germany,Australia)and selected provincial markets in China(Shandong,Zhejiang).It systematically analyzes their underlying causal mechanisms,assesses their multidimensional impacts,and comparatively examines the mitigation strategies adopted across these diverse markets.The study reveals that the core driver of negative prices is the concentrated high generation feed-in from RES during low-load periods.This is concurrently influenced by multiple factors,including insufficient system flexibility,inter-zonal transmission congestion,and constraints imposed by specific market rules such as subsidies and bidding strategies.Major international electricity markets are widely exploring mitigation measures such as optimizing market clearing mechanisms,incentivizing energy storage and demand-side response,and adjusting subsidy policies.In contrast,the occurrence of negative prices in China’s electricity markets,while sharing common drivers,also exhibits complex correlations with domestic factors,notably medium-and long-term contracts and market power dynamics.Consequently,formulating and implementing differentiated and systematic mitigation strategies,drawing upon international experience while being tailored to China’s specific national context,is crucial for the long-term sustainable development of its electricity markets. 展开更多
关键词 Negative electricity price Electricity market Causes and impact Response strategy
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Probabilistic Risk model of Ship Allision Accidents with Offshore Platforms 认领 引用 被引量：1
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作者 Utkarsh Bhardwaj Angelo Palos Teixeira C.Guedes Soares 《哈尔滨工程大学学报（英文版）》 CSCD 2026年第3期713-727,共15页
This paper proposes a Bayesian Network-based framework for risk assessment and probability estimation of vessel-platform allision accidents,using a novel technique that derives probabilities from incidental data.A dat... This paper proposes a Bayesian Network-based framework for risk assessment and probability estimation of vessel-platform allision accidents,using a novel technique that derives probabilities from incidental data.A dataset of 557 allision incidents collected from multiple open source agencies is analysed to identify causation patterns.Basic causes could only be determined for 375 incidents,with supply vessels involved in 61%of cases.Statistical analysis revealed that vessel type and the month of occurrences are significantly associated,and most incidents arose during cargo transfer operations.Fixed installation accounted for the majority of allisions with moving vessels,and human error emerged as the leading contributor(30%).Building on these insights,a Bayesian Network model is developed incorporating 42 identified causes,three causal factors and four consequence levels.Using a recent probabilistic approach,probabilities of basic causes are derived from annual allision occurrence rates.The BN model is then applied to predict annual allision probabilities and to conduct sensitivity analyses.Results show that weather-related causes and misalignment errors exert the strongest influences on accident probabilities.The methodology is transparent and holistic in providing better discernment of the causation probability of allision accidents. 展开更多
关键词 Vesselplatform allision accidents Bayesian networks Causal factors Basic causes Sensitivity analysis
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Combined liver resection and peritoneal stripping for Echinococcus multilocularis 认领 引用
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作者 Kaitlyn R Musto Kristopher P Croome 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS CSCD 2026年第1期97-99,共3页
Echinococcus is a zoonotic parasite and 1 of 17 neglected tropical diseases with a worldwide distribution.The World Health Organization(WHO)targeted for control or elimination by 2050[1,2].Two main species of Echinoco... Echinococcus is a zoonotic parasite and 1 of 17 neglected tropical diseases with a worldwide distribution.The World Health Organization(WHO)targeted for control or elimination by 2050[1,2].Two main species of Echinococcus infect humans:Echinococcus granulosus,causing cystic echinococcosis(CE),and Echinococcus multilocularis(EM),causing alveolar echinococcosis(AE)[3].AE is much rarer but far more severe than CE and ranks as one of the most dangerous helminthic zoonoses in the world[4,5].Humans are rare aberrant intermediate hosts and typically become infected through the ingestion of EM eggs shed in the feces of definitive hosts[6].After an incubation period of many years,humans may develop AE[7].While slow-growing,AE is a devastating clinical condition characterized by silent progression and infiltrative proliferation of the parasite,mimicking a malignancy[7].Without appropriate treatment,AE has a death rate of more than 90%within 10 years of diagnosis[5].For this reason,AE must be considered in the differential diagnosis of patients presenting with a hepatic mass or malignancy. 展开更多
关键词 Echinococcus multilocularis echinococcus multilocularis em causing neglected tropical diseases Liver resection cystic echinococcosis ce helminthic zoonoses alveolar echinococcosis ae ae Peritoneal stripping
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A case of isolated hepatic actinomycosis causing right pulmonary empyema 认领 引用 被引量：2
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作者 Gonenc Kocabay Atahan Cagatay +3 位作者 Haluk Eraksoy Betul Tiryaki Aydin Alper Semra Calangu 《Chinese Medical Journal》 SCIE CAS 2006年第13期1133-1135,共3页
The clinical picture of actinomycosis was first described in 1878.Actinomycosis agents are found in the natural flora of the oral cavity, upper gastrointestinal system and female genital systems. Actinomyces israelii ... The clinical picture of actinomycosis was first described in 1878.Actinomycosis agents are found in the natural flora of the oral cavity, upper gastrointestinal system and female genital systems. Actinomyces israelii is usually responsible for the infections and causes chronic suppurative and granulomatous infections. The most common disease form is cervicofascial infection. Liver is involved in 5% of all actinomycosis infections. Liver involvement secondary to any primary infection site in the abdomen frequently occurs. In 15% of abdominal infections the liver is also involved. However, in very rare cases, a primary focus cannot be found, and these cases are called primary or isolated hepatic actinomycosis （IHA）. IHA which is frequently seen as a solitary abscess can be confused with a malignancy both clinically and radiologically. 展开更多
关键词 A case of isolated hepatic actinomycosis causing right pulmonary empyema oral
对虾致玻璃苗弧菌多克隆抗体制备与胶体金免疫层析试纸条的研制 认领 引用
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作者 杨昊璇 王一周 +3 位作者 丁晓辉 姜伟民 王雅丽 胡鲲 《淡水渔业》 CSCD 北大核心 2026年第4期60-71,共12页
致玻璃苗弧菌(Vibrio spp.causing TPV,VTPV)是近年来严重威胁对虾养殖业的玻璃苗弧菌病重要病原之一,本研究主要开展了VTPV多克隆抗体的制备及免疫学特性评价,并研制了VTPV胶体金免疫层析试纸条,为VTPV的早期监测与快速诊... 致玻璃苗弧菌(Vibrio spp.causing TPV,VTPV)是近年来严重威胁对虾养殖业的玻璃苗弧菌病重要病原之一,本研究主要开展了VTPV多克隆抗体的制备及免疫学特性评价,并研制了VTPV胶体金免疫层析试纸条,为VTPV的早期监测与快速诊断提供了一种新的技术手段。实验采用VTPV灭活菌免疫兔制备VTPV的多克隆抗体,并建立间接ELISA检测体系以对抗体性能进行系统评价。交叉反应实验结果表明,该抗体对VTPV具有最强识别能力(P/N=18.65),对副溶血弧菌和溶藻弧菌分别产生38.39%和27.06%的交叉反应率,对嗜水气单胞菌、温和气单胞菌、维氏气单胞菌及假单胞菌等其他常见水产病原菌均未出现明显反应,表明该抗体在弧菌属内部存在一定程度的交叉识别,但整体仍具有较好的检测特异性。在获得具有较高反应活性的多克隆抗体基础上,进一步构建了VTPV胶体金免疫层析试纸条,并对其检测性能进行了系统评价。通过对不同浓度菌悬液进行梯度检测,确定该试纸的最低可见检出限为1.0×104CFU/mL。特异性实验结果显示,试纸条对VTPV呈现强阳性反应(L4.5~L5),而对副溶血弧菌和溶藻弧菌仅表现为较弱显色(L1.5~L2),对嗜水气单胞菌、气单胞菌属其他菌株、假单胞菌等菌株均未出现明显反应。稳定性实验结果表明,该试纸在180 d储存周期内检测性能总体保持稳定。进一步通过与qPCR分子检测技术对临床真实样本(n=119)进行平行比对,结果显示该试纸条的诊断特异度高达100.00%,总符合率为96.64%,与qPCR结果的Kappa值达0.86。综上所述,本研究建立了胶体金免疫层析快速检测技术,实现了对致玻璃苗弧菌的快速可视化检测。 展开更多
关键词 致玻璃苗弧菌(Vibrio spp.causing TPV) 多克隆抗体 胶体金免疫层析试纸条 快速检测 对虾病害
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Evolution and Causes of the Convective Structure and Microphysical Characteristics of Super Typhoon Saola 认领 引用
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作者 XIAO Liu-si ZHANG Hua-long +3 位作者 WU Nai-geng FENG Lu ZHANG A-si CHEN Sheng 《Journal of Tropical Meteorology》 SCIE CAS CSCD 2026年第2期158-175,共18页
Guangdong Province experienced heavy to extremely heavy rainstorms during Super Typhoon Saola(2023).Areas such as Yunfu,which are typically drier,experienced anomalously extreme rainfall during this event.We analyzed ... Guangdong Province experienced heavy to extremely heavy rainstorms during Super Typhoon Saola(2023).Areas such as Yunfu,which are typically drier,experienced anomalously extreme rainfall during this event.We analyzed the convective structure and microphysical characteristics of the typhoon using dual-polarization radar,disdrometer,and ERA5 reanalysis data.The results show that following Saola’s second landfall,convection redeveloped on the northern side,which was characterized by rich mid-level ice particles and low rain mixing ratios.This resulted in a phase difference in the precipitation particles along Saola’s path.Stations that recorded heavy rain were categorized into four types based on raindrop spectral,spatial,and temporal variables by employing the K-means clustering algorithm.Specifically,the particles observed at Doumen(DM)were classified into a different category from those observed at Yangchun(YC)and Luoding(LD).The microphysical characteristics of raindrop particles evolve under the influence of vertical motion.At DM,the convective structure exhibited a single center of vertical motion with fewer high-altitude ice particles,resulting in a uniform oceanic raindrop type.By contrast,YC was sequentially influenced by two vertically separated centers of vertical motion in the upper and lower atmospheres.These centers alternately dominated the ice-phase and warm-rain processes,resulting in a complex raindrop evolution that transitioned from an intermediate state to a continental type before rapidly reverting to an oceanic type.At LD,the structure showed more pronounced baroclinicity with a higher concentration of water vapor in lower levels,with raindrops tending towards an intermediate continental type.The evolution of vertical motion was driven by changes in environmental conditions.The addition of cold air complicated raindrop evolution in western Guangdong Province compared with that in the Pearl River Estuary.The upper-level vertical-motion center,induced by the uplifting effect of the frontal zone associated with cold air,promoted the development of ice-phase processes and an increase in raindrop size.Subsequently,the lower-level upward-motion center became dominant,which enhanced precipitation concentration.A unique circulation pattern created by the interaction of twin typhoons with each other and with cold air intensified the low-level easterly winds and moisture influx,contributing to extreme rainfall in a typically drier region.Orographic convergence and uplift also played a role in the microphysical evolution of raindrops. 展开更多
关键词 typhoon convective structure microphysical characteristics evolution and causes
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Kumtag 061:A heavily shocked brachinite 认领 引用
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作者 Yuwei Zhang Dongliang Zhang +5 位作者 Thomas Smith P.M.Ranjith Huaiyu He Guangming Song Yan Fan Shijie Li 《Acta Geochimica》 EI CAS CSCD 2026年第1期86-98,共13页
Brachinite is a group of primitive achondrites that enables investigating the evolution of asteroids not fully diff erentiated in the early stage of the solar system.Kumtag 061 is a new meteorite sample collected on O... Brachinite is a group of primitive achondrites that enables investigating the evolution of asteroids not fully diff erentiated in the early stage of the solar system.Kumtag 061 is a new meteorite sample collected on October 27,2019,in Kumtag Desert,Xinjiang Province,China.The oxygen isotope composition(δ18O=5.086‰,δ17O=2.396‰,Δ’17O=-0.298‰)and petrologic and mineralogic analysis suggest Kumtag 061 is a heavy-impacted brachinite(S4-S5).The geochemical composition suggests Kumtag 061 represents a partial melting residue of the brachinite parent body.Based on the noble gas composition,the cosmic ray exposure age of Kumtag 061 is 60.9±9.0 Ma.Combined with the gas retention ages,they indicate a(series of)thermal events on the parent body of brachinites before Kumtag 061 was ejected into space. 展开更多
关键词 Brachinite Impaction caused features Partial melting residues of the parent body Noble gases
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Advances in monogenic female infertility 认领 引用
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作者 Hao Gu Lei Wang Qing Sang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2026年第4期555-576,共22页
Human reproduction requires the fertilization of a mature oocyte with a sperm to form a high-quality embryo.Oogenesis,folliculogenesis,and the activities of the endocrine system play essential roles in female fertilit... Human reproduction requires the fertilization of a mature oocyte with a sperm to form a high-quality embryo.Oogenesis,folliculogenesis,and the activities of the endocrine system play essential roles in female fertility,and disturbances in these processes can result in female infertility,which has become an urgent public health issue worldwide.Genetic studies have identified multiple variants in key genes underlying these processes in infertile females,and these patients are mainly diagnosed with disorders of sex development,premature ovarian insufficiency,congenital hypogonadotropic hypogonadism,central precocious puberty,resistant ovary syndrome,oocyte maturation arrest,fertilization failure,and early embryonic defects.Notably,the known genes account for about 13.2%cases of oocyte and embryo defects(479/3627)and 18.7%cases of premature ovarian insufficiency(193/1030).Here,we review the critical events in female reproduction and highlight the single gene variants with Mendelian inheritance patterns that are responsible for female infertility. 展开更多
关键词 Female infertility Oocyte maturation Folliculogenesis Monogenic causes Mendelian inheritance
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Yttrium-90 downstages giant hepatocellular carcinoma to resectable size 认领 引用
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作者 Qin-Liang Fang Yu Xiong +5 位作者 Ying-Lang Zeng Zi-Qing Zhao Yi-Bin Zhang Yun-Tong Li Zhen-Yu Yin Jian-Yin Zhou 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS CSCD 2026年第2期212-216,共5页
Primary liver cancer is the fourth most common malignancy and the second leading cause of cancer-related death in China[1].Most patients are diagnosed at an advanced stage,which renders them ineligible for curative su... Primary liver cancer is the fourth most common malignancy and the second leading cause of cancer-related death in China[1].Most patients are diagnosed at an advanced stage,which renders them ineligible for curative surgical resection.Thus,improving the resectability rate of hepatocellular carcinoma(HCC)remains a critical challenge in liver cancer research.Selective internal radiotherapy(SIRT)is currently utilized to downstage unresectable HCC,with objective response rates ranging from 85% to 93%[2]. 展开更多
关键词 hepatocellular carcinoma hcc remains second leading cause cancer related death internal radiotherapy sirt yttrium giant hepatocellular carcinoma downstaging primary liver cancer resectable size primary liver cancer
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