期刊文献+
任意字段
题名或关键词
题名
关键词
摘要
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到125篇文章
< 1 2 … 7 >
每页显示 20 50 100
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
显示方式：
Special Topic on Perovskite Solar Ceils 认领 引用
1
《Journal of Semiconductors》 CAS CSCD 2019年第3期18-18,共1页
关键词 Li Special Topic on Perovskite Solar Ceils Zhang
暂未订购 下载PDF
Sericin protects against diabetes-induced injuries in sciatic nerve and related nerve cells 认领 引用 被引量：2
2
作者 Chengjun Song Zhenjun Yang +1 位作者 Meirong Zhong Zhihong Chen 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第6期506-513,共8页
Sericin from discarded silkworm cocoons of silk reeling has been used in different fields, such as cosmetology, skin care, nutrition, and oncology. The present study established a rat model of type 2 diabetes by conse... Sericin from discarded silkworm cocoons of silk reeling has been used in different fields, such as cosmetology, skin care, nutrition, and oncology. The present study established a rat model of type 2 diabetes by consecutive intraperitoneal injections of low-dose （25 mg/kg） streptozotocin. After intragastrical perfusion of sericin for 35 days, blood glucose levels significantly declined, and the expression of neurofilament protein in the sciatic nerve and nerve growth factor in L4-6 spinal ganglion and anterior horn cells significantly increased. However, the expression of neuropeptide Y in spinal ganglion and anterior horn cells significantly decreased in model rats. These findings indicate that sericin protected the sciatic nerve and related nerve cells against injury in a rat type 2 diabetic model by upregulating the expression of neurofilament protein in the sciatic nerve and nerve growth factor in spinal ganglion and anterior horn cells, and downregulating the expression of neuropeptide Y in spinal ganglion and anterior horn cells. 展开更多
关键词 neural regeneration traditional Chinese medicine peripheral nerve injury diabetes mellitus sericin sciatic nerve spinal ganglion ceils anterior horn ceils nerve cells neurofilament proteinnerve growth factor neuropeptide Y streptozotocin photographs-containing paper neuroregeneration
暂未订购 下载PDF
Energy-Efficient Air Conditioning System with Combined a Ceiling Fan for Thermal Comfort in an Office 认领 引用
3
作者 Linlan Chang Win-Jet Luo +3 位作者 Indra Permana Bowo Yuli Prasetyo Alya Penta Agharid Fujen Wang 《Energy Engineering》 EI 2025年第5期1771-1787,共17页
Heating,Ventilation,andAir Conditioning(HVAC)systems are critical formaintaining thermal comfort in office environments which also crucial for occupant well-being and productivity.This study investigates the impact of... Heating,Ventilation,andAir Conditioning(HVAC)systems are critical formaintaining thermal comfort in office environments which also crucial for occupant well-being and productivity.This study investigates the impact of integrating ceiling fans with higher air conditioning setpoints on thermal comfort and energy efficiency in office environments.Field measurements and questionnaire surveys were conducted to evaluate thermal comfort and energysaving potential under varying conditions.Results show that increasing the AC setpoint from 25○C to 27○C,combined with ceiling fan operation,reduced power consumption by 10%,achieving significant energy savings.Survey data confirmed that 85%of participants reported consistent thermal sensations across all conditions,with ceiling fans effectively compensating for higher setpoints through enhanced air circulation.CFDsimulations revealed that mediumspeed ceiling fan operation produced the most uniformairflowdistribution,with an average air velocity of 0.45 m/s,and minimized temperature variations,ensuring balanced thermal conditions.Temperature analysis showed a reduction in hotspots and cold zones,maintaining an average temperature deviation of less than±0.5○C.Predicted Mean Vote(PMV)evaluations at a 27○C setpoint indicated improved thermal comfort,with average PMV values around−0.3,corresponding to a“neutral”thermal sensation.These findings demonstrate the effectiveness of integrating ceiling fans with HVAC systems in achieving energy efficiency and occupant comfort,offering a sustainable approach to reducing AC energy consumption in office environments. 展开更多
关键词 Thermal comfort ceiling fan field measurement questionnaire survey numerical simulation
暂未订购 下载PDF
西门子Artis Zee Ceiling DSA故障维修案例 认领 引用
4
作者 黄耀盛 钟光 +1 位作者 陈俏均 黎子衡 《医疗装备》 2025年第22期110-111,共2页
数字减影血管造影(digital subtraction angiography,DSA)是一种通过数字化处理X线图像、动态显示血管结构的影像技术,其在临床中能够精准诊断血管狭窄、栓塞、动脉瘤等病变,并实时引导介入手术(如支架植入、血栓清除),显著提高微创操... 数字减影血管造影(digital subtraction angiography,DSA)是一种通过数字化处理X线图像、动态显示血管结构的影像技术,其在临床中能够精准诊断血管狭窄、栓塞、动脉瘤等病变,并实时引导介入手术(如支架植入、血栓清除),显著提高微创操作的精准性和安全性,同时降低辐射暴露和造影剂用量[1]。该研究阐述西门子Artis Zee Ceiling DSA结构组成及工作原理,并总结使用过程中出现的两例故障维修案例与同行分享。 展开更多
关键词 数字减影血管造影 Artis Zee Ceiling 数字减影 故障维修
暂未订购 下载PDF
Reproductive toxicity of organic extracts from petrochemical plant effluents discharged to the Yangtze River,China 认领 引用 被引量：9
5
作者 Xiaoyi Wang Wei Shi +5 位作者 Jiang Wu Yingqun Hao Guanjiu Hu Hongling Liu Xiaodong Han Hongxia Yu 《Journal of Environmental Sciences》 SCIE EI CAS 2010年第2期297-303,共7页
Water pollution of the Yangtze River in China became one of challenges that the government is facing today. Increasing numbers of petrochemical plants were built along the river in past decades, and numbers of organic... Water pollution of the Yangtze River in China became one of challenges that the government is facing today. Increasing numbers of petrochemical plants were built along the river in past decades, and numbers of organic chemicals were discharged into the river. Our goal was to establish in vitro system on rat sertoli cells, spermatogenic cells and leydig cells to investigate the reproductive toxicity potential induced by organic extracts from petrochemical effluents. Our results showed that the organic extract depressed the viability （p 〈 0.01） and destroyed the plasma membrane integrity of sertoli cells and spermatogenic cells to a certain degree. Accordingly, proportion of early apoptotic sertoli cells and late apoptotic spermatogenic cells increased significantly. Although significant morphological changes were not detected for leydig cells, the extract was observed to inhibit their testosterone production （p 〈 0.01）. Sertoli cells and sperrnatogenic cells appeared to be more sensitive and maybe the main targets of the key toxins. These results suggested that the in vitro system on rat testicular cells may be useful to predicate reproductive toxicity potential of organic extracts from petrochemical effluents. More attention should be paid to the petrochemical effluents, because long-term accumulation of these organic compounds in organisms may cause spermatogenesis malfunction and testosterone reduction. 展开更多
关键词 wastewater in vitro system sertoli cells spermatogenic ceils leydig cells
暂未订购 下载PDF
The Three-Dimensional Collagen Scaffold Improves the Sternness of Rat Bone Marrow Mesenchymal Stem Cells 认领 引用 被引量：7
6
作者 Sufang Han Yannan Zhao +4 位作者 Zhifeng Xiao Jin Han Bing Chen Lei Chen Jianwu Dai 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2012年第12期633-641,共9页
Mesenchymal stem cells （MSCs） show the great promise for the treatment of a variety of diseases because of their self-renewal and multipotential abilities. MSCs are generally cultured on two-dimensional （2D） subst... Mesenchymal stem cells （MSCs） show the great promise for the treatment of a variety of diseases because of their self-renewal and multipotential abilities. MSCs are generally cultured on two-dimensional （2D） substrate in vitro. There are indications that they may simultaneously lose their sternness and multipotentiality as the result of prolonged 2D culture. In this study, we used three-dimensional （3D） collagen scaffolds as rat MSCs cartier and compared the properties of MSCs on 3D collagen scaffolds with monolayer cultured MSCs. The results demonstrated that collagen scaffolds were suitable for rat MSCs adherence and proliferation. More importantly, compared to MSCs under 2D culture, 3D MSCs significantly maintained higher expression levels of stemness genes （Oct4, Sox2, Rex-1 and Nanog）, yielded high frequencies of colony-forming units-fibroblastic （CFU-F） and showed enhanced osteogenic and adipogenic differentiation efficiency upon induction. Thus, 3D collagen scaffolds may be beneficial for expanding rat MSCs while maintaining the stem cell properties in vitro. 展开更多
关键词 Three-dimensional culture Mesenchymal stem ceils Collagen scaffold Stemness
暂未订购 下载PDF
Anticancer Effect of Icaritin on Human Lung Cancer Cells through Inducing S Phase Cell Cycle Arrest and Apoptosis 认领 引用 被引量：23
7
作者 郑倩 刘伟伟 +5 位作者 李斌 陈慧洁 祝文山 杨广笑 陈明洁 何光源 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2014年第4期497-503,共7页
Icaritin, a prenylflavonoid derivative from Epimedium Genus, has been shown to exhibit many pharmacological and biological activities. However, the function and the underlying mechanisms of icaritin in human non-small... Icaritin, a prenylflavonoid derivative from Epimedium Genus, has been shown to exhibit many pharmacological and biological activities. However, the function and the underlying mechanisms of icaritin in human non-small cell lung cancer have not been fully elucidated. The purpose of this study was to investigate the anticancer effects of icaritin on A549 cells and explore the underlying molecular mechanism. The cell viability after icaritin treatment was tested by MTT assay. The cell cycle distribution, apoptosis and reactive oxygen species（ROS） levels were analyzed by flow cytometry. The mRNA and protein expression levels of the genes involved in proliferation and apoptosis were respectively detected by RT-PCR and Western blotting. The results demonstrated that icaritin induced cell cycle arrest at S phase, and down-regulated the expression levels of S regulatory proteins such as Cyclin A and CDK2. Icaritin also induced cell apoptosis characterized by positive Hoechst 33258 staining, accumulation of the Annexin V-positive cells, increased ROS level and alteration in Bcl-2 family proteins expression. Moreover, icaritin induced sustained phosphorylation of ERK and p38 MAPK. These findings suggested that icaritin might be a new potent inhibitor by inducing S phase arrest and apoptosis in human lung carcinoma A549 cells. 展开更多
关键词 icaritin A549 ceils apoptosis ERK p38 MAPK
暂未订购 下载PDF
Derivation and characterization of human embryonic stem cell lines from poor quality embryos 认领 引用 被引量：4
8
作者 Weiqiang Liu Yifei Yin +12 位作者 Xiaolin Long Yumei Luo Yonghua Jiang Wenhong Zhang Hongzi Du Shaoying Li Yuhong Zheng Qing Li Xinjie Chen Baoping Liao Guohong Xiao Weihua Wang Xiaofang Sun 《Journal of Genetics and Genomics》 SCIE CAS 2009年第4期229-239,共11页
Poor quality embryos discarded from in vitro fertilization(IVF)laboratories are good sources for deriving human embryonic stem cell(hESC)lines.In this study,166 poor quality embryos donated from IVF centers on day 3 w... Poor quality embryos discarded from in vitro fertilization(IVF)laboratories are good sources for deriving human embryonic stem cell(hESC)lines.In this study,166 poor quality embryos donated from IVF centers on day 3 were cultured in a blastocyst medium for 2 days,and 32 early blastocysts were further cultured in a blastocyst optimum culture medium for additional 2 days so that the inner cell masses(ICMs)could be identified and isolated easily.The ICMs of 17 blastocysts were isolated by a mechanical method,while those of the other 15 blastocysts were isolated by immunosurgery.All isolated ICMs were inoculated onto a feeder layer for subcultivation.The rates of ICM attachment,primary ICM colony formation and the efficiency of hESC derivation were similar between the ICMs isolated by the two methods(P〉0.05).As a result,four new hESC lines were established.Three cell lines had normal karyotypes and one had an unbalanced Robertsonian translocation.All cell lines showed normal hESC characteristics and had the differentiation ability.In conclusion,we established a stable and effective method for hESC isolation and culture,and it was confirmed that the mechanical isolation was an effective method to isolate ICMs from poor embryos.These results further indicate that hESC lines can be derived from poor quality embryos discarded by IVF laboratories. 展开更多
关键词 embryonic stem ceils poor quality embryos inner cell mass mechanical isolation immunosurgery
暂未订购 下载PDF
Ginsenoside Rb1 relieves glucose fluctuationinduced oxidative stress and apoptosis in Schwann cells 认领 引用 被引量：3
9
作者 Bing Xue Lianqing Sun +4 位作者 Xiaojin Li Xuan Wang Ying Zhang Yiming Mu Linlang Liang 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第30期2340-2346,共7页
Cultured Schwann cells were treated with 5.6 mM and 50 mM glucose alternating every 8 hours to simulate intermittent high glucose. The present study analyzed the neuroprotective effects of 1, 10 and 100 μM ginsenosid... Cultured Schwann cells were treated with 5.6 mM and 50 mM glucose alternating every 8 hours to simulate intermittent high glucose. The present study analyzed the neuroprotective effects of 1, 10 and 100 μM ginsenoside Rbl on oxidative damage and apoptosis in Schwann cells induced by intermittent high glucose. Flow cytometry demonstrated that ginsenoside Rbl reduced intermittent high glucose-mediated reactive oxygen species production. Enzyme linked immunosorbent assay showed that 8-hydroxy-2-deoxy guanosine levels in Schwann cells decreased following ginsenoside Rbl treatment. Quantitative real-time reverse transcription-PCR and western blot assay results revealed that ginsenoside Rbl inhibited intermittent high glucose-upregulated Bax expression, but antagonized intermittent high glucose-downregulated Bcl-2 expression in Schwann cells. These effects were most pronounced with 100 μM ginsenoside Rbl. These results indicate that ginsenoside Rbl inhibits intermittent high glucose-induced oxidative stress and apoptosis in Schwann cells. 展开更多
关键词 ginsenoside Rbt diabetic peripheral neuropathy Schwann ceils oxidative stress apoptosis glucose fluctuation Chinese herb neuroprotection neural regeneration
暂未订购 下载PDF
Germacrone Induces Apoptosis in Human Hepatoma HepG2 Cells through Inhibition of the JAK2/STAT3 Signalling Pathway 认领 引用 被引量：18
10
作者 柳昀熠 郑倩 +10 位作者 方斌 王维 马凤云 Sadia Roshan Amal Banafa 陈明洁 常俊丽 邓小敏 李克秀 杨广笑 何光源 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2013年第3期339-345,共7页
Summary： Previous studies have shown that STAT3 plays a vital role in the genesis and progression of cancer. In this study, we investigated the relationship between the JAK2/STAT3 signalling pathway and germacrone-in... Summary： Previous studies have shown that STAT3 plays a vital role in the genesis and progression of cancer. In this study, we investigated the relationship between the JAK2/STAT3 signalling pathway and germacrone-induced apoptosis in HepG2 cells. HepG2 cells were incubated with germacrone for 24 h, the protein expression of p-STAT3, STAT3, p-JAK2 and JAK2 was detected by Westem Blotting, and RT-PCR was used to determine the expression of STAT3, p53, Bcl-2 and Bax at transcriptional levels. Besides that, HepG2 cells were pre-treated with AG490 or IL-6 for 2 h, and then incubated with ger- macrone for 24 h. The expression ofp-JAK2, JAK2, p-STAT3, STAT3, p53, Bax and Bcl-2 was detected by Western blotting. The activity of HepG2 cells was tested by MTT assay. The apoptosis of HepG2 cells and levels of reactive oxygen species （ROS） were flow cytometrically measured. The results showed that germacrone exposure decreased p-STAT3 and p-JAK2 and regulated expression of p53 and Bcl-2 family members at the same time. Moreover, IL-6 enhanced the activation of the JAK2/STAT3 signalling pathway and therefore attenuated the germacrone-induced apoptosis. Suppression of JAK2/STAT3 signalling pathway by AG490, an inhibitor of JAK2, resulted in apoptosis and an increase in ROS in response to germacrone exposure. We therefore conclude that germacrone induces apoptosis through the JAK2/STAT3 signalling pathway. 展开更多
关键词 germacrone JAK2/STAT3 apoptosis HepG2 ceils
暂未订购 下载PDF
Purification,characterization,in vitro anti-hepatic fibrosis activity of bioactive peptides derived from Carapax Trionycis hydrolysates 认领 引用 被引量：6
11
作者 胡春玲 彭小芝 +3 位作者 唐尹萍 刘焱文 胡祖良 高建蓉 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2017年第8期605-610,共6页
Anti-hepatic fibrosis peptide from Carapax Trionycis was purified, characterized, and inhibitory effect was assessed. Carapax Trionycis extract peptide hydrolysates （CTEPHs） were separated by ultrafiltration, Sephad... Anti-hepatic fibrosis peptide from Carapax Trionycis was purified, characterized, and inhibitory effect was assessed. Carapax Trionycis extract peptide hydrolysates （CTEPHs） were separated by ultrafiltration, Sephadex G-15 gel chromatography and RP-HPLC. One novel anti-hepatic fibrosis peptide （CTEPH-I： Asn-Pro-Asn-Pro-Thr） was obtained and identified. MTS assay and enzyme-linked immunosorbent assay were applied to evaluate the anti-fibrotic effect of CTEPH-1 on activated hepatic stellate cells （HSCs） in vitro. CTEPH-1 efficiently inhibited activation and proliferation of cultured HSC-T6 cells via lowering the contents of collagen and TIMP- 1 except for matrix metalloproteinase- 1 （MMP- 1）. The purified peptide might be beneficial as functional food or potential drug for treatment of liver fibrogenesis. 展开更多
关键词 Carapax Trionycis extract peptide hydrolysates Extracellular matrix Hepatic stellate ceils
暂未订购 下载PDF
Circulating tumor cell isolation:the assets of filtration methods with polycarbonate track-etched filters 认领 引用 被引量：6
12
作者 Claire Dolfus Nicolas Piton +1 位作者 Emmanuel Toure Jean-Christophe Sabourin 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2015年第5期479-487,共9页
Circulating tumor cells （CTCs） arise from primary or secondary tumors and enter the bloodstream by active or passive intravasation. Given the low number of CTCs, enrichment is necessary for detection. Filtration met... Circulating tumor cells （CTCs） arise from primary or secondary tumors and enter the bloodstream by active or passive intravasation. Given the low number of CTCs, enrichment is necessary for detection. Filtration methods are based on selection of CTCs by size using a filter with 6.5 to 8 pm pores. After coloration, collected CTCs are evaluated according to morphological criteria. Immunophenotyping and fluorescence in situ hybridization techniques may be used. Selected CTCs can also be cultivated in vitro to provide more material. Analysis of genomic mutations is difficult because it requires adapted techniques due to limited DNA materials. Filtration-selected CTCs have shown prognostic value in many studies but multicentric validating trials are mandatory to strengthen this assessment. Other clinical applications are promising such as follow-up, therapy response prediction and diagnosis. Microfluidic emerging systems could optimize filtration-selected CTCs by increasing selection accuracy. 展开更多
关键词 Circulating tumor ceils （CTCs） biological markers filtration
暂未订购 下载PDF
Toxic effects of Karenia mikimotoi extracts on mammalian cells 认领 引用 被引量：5
13
作者 陈洋 颜天 +1 位作者 于仁成 周名江 《Chinese Journal of Oceanology and Limnology》 CSCD 2011年第4期860-868,共9页
Karenia is one of the most harmful and representative red tide genus in a temperate zone. Blooms caused by this genus have resulted in massive fish death in the South China Sea and the East China Sea. However, the pot... Karenia is one of the most harmful and representative red tide genus in a temperate zone. Blooms caused by this genus have resulted in massive fish death in the South China Sea and the East China Sea. However, the potential effects of this dinoflagellate on human health through the transfer of toxins via marine food webs, and the mechanisms of toxicity, are still unknown. Therefore, we examined the toxic effects of a strain of K. mikimotoi （isolated from the South China Sea） on the proliferation and morphology of four mammalian cell lines （two normal cell lines and two cancer cell lines）. In addition, we carried out a preliminary investigation on the mechanism of toxicity of the alga. The results show that the polar lipid-soluble component ofK. mikimotoi significantly inhibited proliferation of the four cell lines, and resulted in the ceils becoming spherical, swollen and damaged. The result of Annexin V and PI double-staining confirmed that cell membranes were disrupted. The malonaldehyde （MDA） contents in the medium of the four cell lines treated with the polar-lipid extracts all increased significantly, which indicates that the polar-lipid toxins produced by K. rnikimotoi could adversely affect mammalian cells by inducing lipid peroxidation. We conclude that K. mikimotoi is a potential threat to human health, and the comprehensive effect of this dinoflagellate and its mechanisms should be investigated further. 展开更多
关键词 Karenia mikimotoi mammalian ceils super-oxidation
暂未订购 下载PDF
Mechanism of Erhuang capsule for treatment of multiple sclerosis 认领 引用 被引量：4
14
作者 Kangning Li Yongping Fan +1 位作者 Tao Yang Lei Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第6期523-531,共9页
Erhuang capsule, a typical formula based on traditional Chinese medicine theory, is widely used to ameliorate multiple sclerosis, inflammation and side effects of glucocortJcoid treatment. Oligodendrocyte precursor ce... Erhuang capsule, a typical formula based on traditional Chinese medicine theory, is widely used to ameliorate multiple sclerosis, inflammation and side effects of glucocortJcoid treatment. Oligodendrocyte precursor cells are neural stem cells that are important for myelin repair and regeneration. In the present study, Erhuang capsule effectively improved clinical symptoms and neurological function scores, reduced mortality and promoted recovery of neurological functions of mice with experimental autoimmune encephalomyelitis. The mechanism of action involved significant increases in oligodendrocyte precursor cell proliferation in specific regions of the brain and spinal cord, increased oligodendrocyte lineage gene 2 expression and enhanced oligodendrocyte precursor cell differentiation. 展开更多
关键词 neural regeneration traditional Chinese medicine neurodegenerative diseases oligodendrocyteprecursor ceils Erhuang capsule Chinese compound recipe methods of tonifying the kidney resolving phlegm and activating blood oligodendrocyte lineage gene 2 experimental autoimmuneencephalomyelitis multiple sclerosis central nervous system grants-supported paper photographs-containing paper neuroregeneration
暂未订购 下载PDF
Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells 认领 引用 被引量：2
15
作者 Jinghui Zheng Jian Liang +6 位作者 Xin Deng Xiaofeng Chen Fasheng Wu Xiaofang Zhao Yuan Luo Lei Fu Zuling Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第18期1370-1377,共8页
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell diff... Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively, mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction. 展开更多
关键词 Buyang Huanwu decoction bone marrow mesenchymal stem ceils extracellular signal-regulatedprotein kinase mitogen-activated protein kinase signaling pathway neuron specific enolase nestin cell signal transduction pathway neural regeneration
暂未订购 下载PDF
Over-expression of VEGF and MMP-9 in Residual Tumor Cells of Hepatocellular Carcinoma after Embolization with Lipidol 认领 引用 被引量：6
16
作者 石玉龙 徐涛 +1 位作者 李乐平 陈孝平 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2013年第1期90-95,共6页
The expression and implication of vascular endothelial growth factor （VEGF） and matrix metalloproteinase-9 （MMP-9） in residual hepatic tumor cells after lipiodol embolization were investi- gated. Two weeks after t... The expression and implication of vascular endothelial growth factor （VEGF） and matrix metalloproteinase-9 （MMP-9） in residual hepatic tumor cells after lipiodol embolization were investi- gated. Two weeks after transplantation of VX2 tumor cells into the livers of rabbits, a xenograft model of the human hepatic neoplasm was successfully established. Forty rabbits were randomly divided into control group （n=20） and lipiodol group （n=20）. For the control group, 1 mL normal saline was injected through the gastroduodenal artery, whereas 0.3 mL/kg lipiodol was applied for the lipiodol group. One week after embolization, the expression level of VEGF in the plasma was measured by using en- zyme-linked immunosorbent assay （ELISA）. A three-step immunohistochemieal technique （ABC） was employed to detect the protein levels of VEGF and MMP-9 and the quantitative PCR for their mRNA levels was performed in the residual tumor cells. The VEGF in the plasma was significantly higher in the lipiodol group （1.42~0.29 ng/mL） than in the control group （1.12~0.21 ng/mL） （P〈0.01）. Moreover, the positive rate of VEGF protein in the residual tumor cells was significantly higher in the lipiodol group （62.13%~7.69%） than in the control group （53.16%~9.17%） （P〈0.05）. Similarly, the MMP-9 ex- pression in the residual ~mor cells was higher in the lipiodol group. The mRNA levels of VEGF （2.9313~2.4231） and MMP-9 （3.5721~1.6107） in the lipiodol group were significantly higher than those in the control group （1.5728~0.9453 and 1.7573~1.0641, respectively, P〈0.05）. Therefore, it was rea- sonable to speculate that the increased expression of VEGF and MMP-9 in residual hepatic tumor cells and tumor angiogenesis post-embolization would be responsible for the increased metastatic potentiality and invasiveness of these cells. 展开更多
关键词 vascular endothelial growth factor matrix metalloproteinase-9 embolization residual he- patic tumor ceils metastasis
暂未订购 下载PDF
Effect of IKVAV Peptide Nanofiber on Proliferation,Adhesion and Differentiation into Neurocytes of Bone Marrow Stromal Cells 认领 引用 被引量：3
17
作者 吴斌 郑启新 +2 位作者 吴永超 郭晓东 邹枕玮 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2010年第2期178-182,共5页
This study examined the effect of IKVAV peptide nanofiber on proliferation, adhesion and differentiation into neurocytes of bone marrow stromal cells （BMSCs）. IKVAV Peptide-amphiphile was synthesized and purified. T... This study examined the effect of IKVAV peptide nanofiber on proliferation, adhesion and differentiation into neurocytes of bone marrow stromal cells （BMSCs）. IKVAV Peptide-amphiphile was synthesized and purified. Then, hydrogen chloride was added to the diluted aqueous solutions of PA to induce spontaneous formation of nanofiber in vitro. The resultant samples was observed tmder transmission electron microscope. BMSCs were cultured with IKVAV peptide nanofiber. The effect of IKVAV nanofiber on the proliferation, adhesion and induction differentiation of BMSCs was observed by inverted microscopy, calcein-AM/PI staining, cell counting and immunofluorescence staining. The results demonstrated that IKVAV peptide-amphiphile could self-assemble to form nanofiber gel. BMSCs cultured in combination with IKVAV peptide nanofiber gel grew well and the percentage of live cells was over 90%. IKVAV peptide nanofiber gel exerted no influence on the proliferation of BMSCs and could promote the adhesion of BMSCs and raise the ra- tio of neurons when BMSCs were induced to differentiate into neurocytes. It is concluded that BMSCs could proliferate and adhere well and yield more neurons during when induced to differente into neurocytes on IKVAV peptide nanofiber gel. 展开更多
关键词 bone marrow stem ceils IKVAV self-assembly nerve tissue engineering
暂未订购 下载PDF
Effects of Estrogen-related Receptor alpha (ERRα) on Proliferation and Metastasis of Human Lung Cancer A549 Cells 认领 引用 被引量：3
18
作者 黄建伟 管保章 +6 位作者 尹良红 刘璠娜 胡波 郑绮宜 李佛兰 钟影雪 陈宇 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2014年第6期875-881,共7页
Estrogen-related receptor alpha （ERRα） plays an important role in the development of hor- monezdependent cancers, but its roles in lung cancer remain elusive. The present study was aimed to investigate the effects ... Estrogen-related receptor alpha （ERRα） plays an important role in the development of hor- monezdependent cancers, but its roles in lung cancer remain elusive. The present study was aimed to investigate the effects of ERRα on the proliferation and metastasis of lung cancer A549 cells. The mRNA and protein levels of ERRor were detected in lung cancer A549 and MCF-7 cells and bronchial epithelial BEAS-2B cells by qRT-PCR and Western blotting, respectively. ERRor plasmid transfection and XCT-790 （an inverse agonist of ERRc0 were used to up-regulate or down-regulate ERRα expression in A549 cells, respectively. The viability of A549 cells was measured by cell counting kit-8 （CCK-8） and the motility of A549 cells by wound healing assay and Transwell migration/invasion assay. The epithelial markers E-cadherin （E-Cad） and zona occludin-1 （ZO-1）, the mesenchymal markers fi- bronectin （FN） and vimentin （Vim） and the transcription factors （Snail, Zebl Twist and Slug） were fur- ther detected at mRNA and protein levels by qRT-PCR and Western blotting, respectively. The results showed that ERRor promoted the growth of lung cancer A549 cells in vitro. XCT-790 significantly in- hibited the migration and invasion of A549 cells. Over-expression of ERRα promoted the epithe- lial-to-mesenchymal transition （EMT） of A549 ceils, down-regulated the epithelial makers E-Cad and ZO-1, and up-regulated the mesenchymal makers FN and Vim. Silencing of Slug, but not other tran- scription factors, significantly abolished the ERRs-induced EMT of A549 cells. It was suggested that ERRor promoted the migration and invasion of A549 cells by inducing EMT, and Slug was involved in the process. Targeting ERRor might be an efficient approach for lung cancer treatment. 展开更多
关键词 estrogen-related receptor alpha XCT-790 migration invasion A549 ceils
暂未订购 下载PDF
Presence of circulating tumor cells is associated with metabolic-related variables in postoperative patients with early-stage breast cancer 认领 引用 被引量：1
19
作者 Yumei Shi Guochun Zhang +5 位作者 Yulei Wang Chongyang Ren Lingzhu Wen Wenzhen Zhu Xiaoqing Chen Ning Liao 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2018年第3期340-350,共11页
Objective：Although circulating tumor cells（CTCs）have been well-established as promising prognostic biomarkers in both early breast cancer and metastatic settings,little is known regarding the prognostic relevance o... Objective：Although circulating tumor cells（CTCs）have been well-established as promising prognostic biomarkers in both early breast cancer and metastatic settings,little is known regarding the prognostic relevance of CTCs in the long-term postoperative monitoring of patients with non-metastatic breast cancer（non-MBC）.In this study,we investigated the associations of CTCs with clinicopathological features and metabolic-related variables,such as obesity and hyperglycemia.Methods：In this retrospective study,we recruited 264 patients with postoperative stage Ⅰ–Ⅲ breast cancer at Guangdong General Hospital from January 2009 to December 2015.The prevalence and number of CTCs were assessed using the Cell Search System at a median time of 19.0 months[interquartile range（IQR）,7.8–33.0]after surgery.The CTC assay results were correlated with the clinicopathological features and metabolic-related variables.A multivariate logistic regression analysis was performed to further determine the independent predictors of CTCs.Results：CTCs were detected in 10.6%of all patients.The positive rate of CTCs in patients with infiltrating ductal carcinoma was lower than that in patients with other pathological types（9.0%vs.28.6%,P=0.020）.More importantly,the presence of CTCs was correlated with blood glucose level（P=0.015）and high-density lipoprotein level（P=0.030）.The multivariate logistic regression analysis showed that the pathological type[odds ratio（OR）：1.757,95%CI：1.021–3.023;P=0.042]and blood glucose level（OR：1.218,95%CI：1.014–1.465;P=0.035）were independent predictors of the presence of CTCs.Conclusions：This study revealed potential associations between CTCs and metabolic-related factors in Chinese patients with non-MBC and supports the hypothesis that metabolic dysfunction in breast cancer patients might influence the biological activity of metastatic breast cancer,leading to a higher prevalence of CTCs. 展开更多
关键词 Breast cancer circulating tumor ceils hyperglycemia metabolic-related variables
暂未订购 下载PDF
Mitofusin2 Decreases Intracellular Cholesterol of Oxidized LDL-Induced Foam Cells from Rat Vascular Smooth Muscle Cells 认领 引用 被引量：2
20
作者 贺超 陈颖 +3 位作者 刘纯 操明 范玉璟 郭小梅 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2013年第2期212-218,共7页
Mitofusin2 （Mfn2） plays a pivotal role in the proliferation and apoptosis of vascular smooth muscle cells （VSMCs）. The purpose of this study was to investigate the effects of Mfn2 on the traffick- ing of intracell... Mitofusin2 （Mfn2） plays a pivotal role in the proliferation and apoptosis of vascular smooth muscle cells （VSMCs）. The purpose of this study was to investigate the effects of Mfn2 on the traffick- ing of intracellular cholesterol in the foam ceils derived from rat VSMCs （rVSMCs） and also to investigate the effects of Mfn2 on the expression of adenosine triphosphate-binding cassette subfamily A member 1 （ABCA1）, adenosine triphosphate-binding cassette subfamily G member 1 （ABCG1） and peroxisome proliferator-activated receptor gamma （PPARy）. The rVSMCs were co-cultured with oxi- dized low density lipoprotein （LDL, 80 ~tg/mL） to produce foam cells and cholesterol accumulation in cells. Before oxidized LDL treatment, different titers （20, 40 and 60 pfu/cell） of recombinant adenovirus containing Mfn2 gene （Adv-Mfn2） were added into the culture medium for 24 h to transfect the Mfn2 gene into the rVSMCs. Then the cells were harvested for analyses. The protein expression of Mfn2 was significantly higher in Adv-Mfn2-transfected group than in untransfected group （P〈0.05）, and the ex- pression levels significantly increased when the titer of Adv-Mfn2 increased （P〈0.05）. At 24 or 48 h af- ter oxidized LDL treatment, rVSMCs became irregular and their nuclei became larger, and their plasma abounded with red lipid droplets. However, the number of red lipid droplets was significantly decreased in Adv-Mfn2-transfected group as compared with untransfected group. At 48 h after oxidized LDL treatment, the intracellular cholesterol in rVSMCs was significantly increased （P〈0.05）, but it was sig- nificantly decreased in Adv-Mfn2-transfected group as compared with untransfected group （P〈0.05）, and it also significantly decreased when the titer of Adv-Mfn2 increased （P〈0.05）. The mRNA and pro- tein expression levels of ABCA1 and ABCG1 were significantly increased in Adv-Mfn2-transfected group as compared with untransfected group （P〈0.05）. Though the mRNA and protein expression levels of PPARy was not significantly increased （P〉0.05）, the phosporylation levels of PPARy were signifi- cantly decreased in Adv-Mfn2-transfected group as compared with untransfected group （P〈0.05）. These results suggest that the transfection of Adv-Mfn2 can significantly reduce intracellular cholesterol in oxidized LDL-induced rVSMCs possibly by decreasing PPAR＇/phosporylation and then increasing pro- tein expression levels of ABCAI and ABCG1, which may be helpful to suppress the formation of foam cells. 展开更多
关键词 Mitofusin 2, peroxisome proliferator-activated receptor gamma adenosine triphosphatebinding cassette subfamily A member 1 adenosine triphosphate-binding cassette subfamily G member 1 vascular smooth muscle ceils oxidized low density lipoprotein rats
暂未订购 下载PDF
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
上一页 1 2 … 7 下一页 到第 页
在线咨询 使用帮助 返回顶部 意见反馈