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Generation of rat-mouse chimeras by introducing single cells of rat inner cell masses into mouse blastocysts 认领 引用
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作者 TiANDa Li Leyun Wang +7 位作者 Xinxin Zhang Liyuan Jiang Yufei Li Junjie Mao Tongtong Cui Wei Li Liu Wang Qi Zhou 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2018年第6期325-328,共4页
In the field of developmental biology and regenerative medicine,mammalian interspecific chimeras have been proved very useful for investigating early embryonic development and the immune system establishment,and exten... In the field of developmental biology and regenerative medicine,mammalian interspecific chimeras have been proved very useful for investigating early embryonic development and the immune system establishment,and extended to a promising potential for human organ generation（Rossant et al.,1982）. 展开更多
关键词 Generation of rat-mouse chimeras by introducing single cells of rat inner cell masses into mouse blastocysts GFP
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Chaperone-mediated autophagy targeting chimeras (CMATAC) forthe degradation of ERα in breast cancer 认领 引用 被引量：1
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作者 JUN ZHANG YEHONG HUANG +2 位作者 WENZHUO LIU LULU LI LIMING CHEN 《BIOCELL》 SCIE 2020年第4期591-595,共5页
Estrogen receptor alpha(ERα/ESR1)is overexpressed in over half of all breast cancers and is considered a valuable therapeutic target in ERαpositive breast cancer.Here,we designed a membrane-permeant Chaperonemediate... Estrogen receptor alpha(ERα/ESR1)is overexpressed in over half of all breast cancers and is considered a valuable therapeutic target in ERαpositive breast cancer.Here,we designed a membrane-permeant Chaperonemediated Autophagy Targeting Chimeras(CMATAC)peptide to knockdown endogenous ERαprotein through chaperone-mediated autophagy.The peptide contains a cell membrane-penetrating peptide(TAT)that allows the peptide to by-pass the plasma membrane,anαI peptide as a protein-binding peptide(PBD)that binds specifically to ERα,and CMA-targeting peptide(CTM)that targeting chaperone-mediated autophagy.We validated that ERαtargeting peptide was able to target and degrade ERαto reduce the viability of ERαpositive breast cancer cells.Taken together,our studies provided a new method to reduce the level of intracellular ERαprotein via CMATAC,and thus may provide a new strategy for the treatment of ERαpositive breast cancer. 展开更多
关键词 Chaperone-mediated Autophagy Targeting Chimeras (CMATAC) Breast cancer Peptide ERα
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Generation of rat blood vasculature and hematopoietic cells in rat-mouse chimeras by blastocyst complementation 认领 引用 被引量：4
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作者 Xiaomin Wang Hui Shi +11 位作者 Juanjuan Zhou Qingjian Zou Quanjun Zhang Shixue Gou Pengfei Chen Lisha Mou Nana Fan Yangyang Suo Zhen Ouyang Chengdan Lai Quanmei Yan Liangxue Lai 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2020年第5期249-261,共13页
Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells.A mismatch of the major histocompatibility complex of va... Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells.A mismatch of the major histocompatibility complex of vascular endothelial cells between the human and host animal will cause graft rejection in the transplanted organs.Therefore,to achieve a transplantable organ in animals without rejection,creation of vascular endothelial cells derived from humans within the organ is necessary.In this study,to explore whether donor xeno-pluripotent stem cells can compensate for blood vasculature in host animals,we generated rat-mouse chimeras by injection of rat embryonic stem cells(rESCs)into mouse blastocysts with deficiency of Flk-1 protein,which is associated with endothelial and hematopoietic cell development.We found that rESCs could differentiate into vascular endothelial and hematopoietic cells in the rat-mouse chimeras.The whole yolk sac(YS)of Flk-1^EGFP/ECFP rat-mouse chimera was full of rat blood vasculature.Rat genes related to vascular endothelial cells,arteries,and veins,blood vessels formation process,as well as hematopoietic cells,were highly expressed in the YS.Our results suggested that rat vascular endothelial cells could undergo proliferation,migration,and self-assembly to form blood vasculature and that hematopoietic cells could differentiate into B cells,T cells,and myeloid cells in rat-mouse chimeras,which was able to rescue early embryonic lethality caused by Flk-1 deficiency in mouse. 展开更多
关键词 Blastocyst complementation Interspecies chimera Intraspecies chimera Flk-1 Vascular endothelial cell Hematopoietic cell
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The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras 认领 引用
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作者 郭继彤 李雪峰 +6 位作者 Shahnaz Fida 苟克勉 Nakisa Malakooti ZHANG Chun-fang John R Morrison Alan O Trounson DU Zhong-tao 《Zoological Research》 北大核心 2009年第2期158-164,共7页
The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses （ICMs） and fetal neural stem （FNS） cells. In ... The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses （ICMs） and fetal neural stem （FNS） cells. In result, three rat chimeras were produced by day 5 （D5） Sprague-Dawley （SD） blastocysts injected with ICMs derived from day 6 （D6） and D5 Dark Agouti （DA） blastocysts; four rat chimeras had been generated by D5 DA blastocyst injected with D5 SD ICMs. For the requirement of gene modification, cultured rat inner cell mass cells were assessed to produce chimeras, but no chimeras were generated from injected embryos. The potential to generate chimeras from rFNS and transfected rFNS cells were tested, but no chimeric pups were produced. Only 2 of 41 fetuses derived from D5 DA blastocyst injection with SD LacZ transfected rFNS cells showed very low number of LacZ positive cells in the section. These results indicate that DA and SD rat ICMs arc able to contribute to chimeras, but their potential decreases significantly after culture in vitro （P〈0.05）, and rFNS cells only have the potential to contribute to early fetal development. 展开更多
关键词 Rat chimeras Inner cell mass Rat fetal neural stem cells Blastocyst injection
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Emerging Human Pluripotent Stem Cell-Based Human–Animal Brain Chimeras for Advancing Disease Modeling and Cell Therapy for Neurological Disorders 认领 引用 被引量：1
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作者 Yanru Ji Jenna Lillie McLean Ranjie Xu 《Neuroscience Bulletin》 SCIE CAS CSCD 2024年第9期1315-1332,共18页
Human pluripotent stem cell(hPSC)models provide unprecedented opportunities to study human neurological disorders by recapitulating human-specific disease mechanisms.In particular,hPSC-based human–animal brain chimer... Human pluripotent stem cell(hPSC)models provide unprecedented opportunities to study human neurological disorders by recapitulating human-specific disease mechanisms.In particular,hPSC-based human–animal brain chimeras enable the study of human cell pathophysiology in vivo.In chimeric brains,human neural and immune cells can maintain human-specific features,undergo maturation,and functionally integrate into host brains,allowing scientists to study how human cells impact neural circuits and animal behaviors.The emerging human–animal brain chimeras hold promise for modeling human brain cells and their interactions in health and disease,elucidating the disease mechanism from molecular and cellular to circuit and behavioral levels,and testing the efficacy of cell therapy interventions.Here,we discuss recent advances in the generation and applications of using human–animal chimeric brain models for the study of neurological disorders,including disease modeling and cell therapy. 展开更多
关键词 Human pluripotent stem cell Human–animal chimera Neurological disorder Disease modeling Cell therapy Human neurons and glia Microglia Organoid
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Customizable Aptamer-Engineered Nano-Lysosome-Targeting Chimeras Enable Receptor-Independent and High-Fidelity Degradation of Membrane Protein 认领 引用
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作者 Ran Liu Yangfang Yun +4 位作者 Zhi-Yuan Feng Zhe Feng Wenxian Zhang Yuanzhen Ye Jingjing Zhang 《CCS Chemistry》 CSCD 2026年第3期1480-1494,共15页
Lysosome-targeting chimeras(LYTACs)have emerged as a promising platform for the selective degradation of membrane proteins,but their reliance on specific lysosome-targeting receptors(LTRs)introduces potential off-targ... Lysosome-targeting chimeras(LYTACs)have emerged as a promising platform for the selective degradation of membrane proteins,but their reliance on specific lysosome-targeting receptors(LTRs)introduces potential off-target effects that can disrupt cellular functions.These unintended interactions compromise the therapeutic efficacy of LYTACs,particularly in normal cells where LTRs regulate essential processes.To overcome these limitations,we introduce Polyvalent Aptamer-gold Nanoparticle-assisted TArgeting Chimeras(PANTACs),an innovative LTR-independent strategy for membrane protein degradation.PANTACs harness clathrinmediated endocytosis for efficient cellular internalization and lysosomal degradation,bypassing the need for LTR engagement.This approach enables highly customizable,precise degradation of membrane proteins with minimal disruption to cellular homeostasis.We demonstrate the versatility and high-fidelity degradation of clinically relevant membrane proteins,such as PD-L1 and estrogen receptorα,at nanomolar concentrations,offering a robust alternative to traditional LTR-dependent methods.Our findings establish PANTACs as a versatile and precise tool for membrane protein degradation,offering a promising strategy for therapeutic targeting with minimal off-target effects and high specificity. 展开更多
关键词 lysosome-targeting chimeras aptamer nanoparticle insulin-like growth factor 2 membrane protein
Controlling chaos and supressing chimeras in a fractional-order discrete phase-locked loop using impulse control 认领 引用
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作者 Karthikeyan Rajagopal Anitha Karthikeyan Balamurali Ramakrishnan 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第12期63-73,共11页
A fractional-order difference equation model of a third-order discrete phase-locked loop(FODPLL) is discussed and the dynamical behavior of the model is demonstrated using bifurcation plots and a basin of attraction. ... A fractional-order difference equation model of a third-order discrete phase-locked loop(FODPLL) is discussed and the dynamical behavior of the model is demonstrated using bifurcation plots and a basin of attraction. We show a narrow region of loop gain where the FODPLL exhibits quasi-periodic oscillations, which were not identified in the integer-order model. We propose a simple impulse control algorithm to suppress chaos and discuss the effect of the control step. A network of FODPLL oscillators is constructed and investigated for synchronization behavior. We show the existence of chimera states while transiting from an asynchronous to a synchronous state. The same impulse control method is applied to a lattice array of FODPLL, and the chimera states are then synchronized using the impulse control algorithm. We show that the lower control steps can achieve better control over the higher control steps. 展开更多
关键词 discrete Josephson junction fractional order chaos impulse control chimera
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Production of chicken chimeras by fusing blastodermal cells with electroporation 认领 引用
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作者 S.Aritomi N.Fujihara 《Asian Journal of Andrology》 SCIE CAS 2000年第4期271-275,共5页
Aim: To establish techniques for producing somatic and germline chimeric chicken by transferring blastodermal cellsfused with electroporation. Methods: Stage-X blastodermal cells isolated from freshly laid fertile uni... Aim: To establish techniques for producing somatic and germline chimeric chicken by transferring blastodermal cellsfused with electroporation. Methods: Stage-X blastodermal cells isolated from freshly laid fertile unincubated whiteLeghorn and Rhode Island red chicken eggs were fused with electroporation. The treated cell suspension was transferredto the recovery medium (DMEM containing 10% FBS) and was injected into the subgenninal cavity of recipient unin-cubated embryos (stage X). Results: Of 177 recipient embryos injected with the fusing blastodermal cells, 6(3.4 %) survived to hatching. Somatic chimerism was examined in the melanocyte of the feather. The presence offeathers originating from the donor cell was observed in 1 bird (16.7%) out of the 6 hatched birds. After 21 days ofincubation two birds out of five embryos were subjected to polymerase chain reaction (PCR) analysis for W-chromo-some-specific DNA for each tissue. One bird possessed W-chromosome-specific DNA in the stomach, and the other ex-hibited the same DNA in the left and right gonads and other tissues, but not the stomach. Conclusion: Recipientembryo having electrofused blastodennal cells yields somatic and germline chimeric chickens more successfully.(Asian J Androl 2000 Dec; 2: 271-275) 展开更多
关键词 chicken blastoderm electroporation chimera
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Establishment of Embryonic Stem Cell Lines from C57BL/6J Mice and Generation of Chimeras 认领 引用
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作者 何维 高建刚 +1 位作者 刘晓 孙方臻 《Developmental and Reproductive Biology》 1997年第2期13-20,共8页
Four embryonic stem (ES) cell lines, designated CE1, CE2, CE3 and CE4, were isolated from C57BL/6J blastocysts. The ratio of normal diploid composition of these cell lines is above 70%. To examine the differentiation ... Four embryonic stem (ES) cell lines, designated CE1, CE2, CE3 and CE4, were isolated from C57BL/6J blastocysts. The ratio of normal diploid composition of these cell lines is above 70%. To examine the differentiation potential of the ES cells, the CE2 cells were injected subcutaneously into syngenic mice, and many kinds of differentiated cells were observed on the sections of the teratoma derived from this ES cell line. On the other hand, to test the chimeric ability of the ES cells, the CE2 cells were microinjected into the blastocysts of ICR mice, and a chimera was obtained among living pups. These results show that CE2 ES cells are pluripotent stem cells, which can differentiate into many kinds of cell types, and can be used as a cell system for further research.  展开更多
关键词 C57BL/6J mouse ES cell line establishment chimera.
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Leveraging glypican-3(GPC3)-mediated lysosome-targeting chimeras(GLTACs)for targeted membrane protein degradation 认领 引用
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作者 Bin Yu 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2025年第4期2293-2294,共2页
Targeted protein degradation(TPD)has transformed drug discovery by eliminating disease-causing proteins rather than merely inhibiting their activity.Proteolysis-targeting chimeras(PROTACs)have significantly advanced t... Targeted protein degradation(TPD)has transformed drug discovery by eliminating disease-causing proteins rather than merely inhibiting their activity.Proteolysis-targeting chimeras(PROTACs)have significantly advanced this field by using bifunctional small molecules to recruit E3 ubiquitin ligases to degrade proteins of interest.However,PROTACs,relying on the ubiquitin-proteasome system,predominantly target cytosolic and nuclear proteins,but they struggle to degrade membrane or extracellular proteins.To address this gap,scientists have developed lysosome-targeting chimeras(LYTACs),which consist of an antibody or peptide binding the target protein,linked to a ligand that binds a cellsurface lysosomal trafficking receptor.By bridging a target protein on the cell surface to a lysosome-shuttling receptor,LYTACs are internalized into the cell and delivered to lysosomes,where acidic enzymes degrade various membrane proteins.In essence,LYTACs broaden the druggable proteome,allowing researchers to modulate“undruggable”targets that PROTACs and traditional inhibitors cannot touch. 展开更多
关键词 gltacs protacs ubiquitin proteasome system lysosome targeting chimeras drug discovery targeted protein degradation targeted protein degradation tpd bifunctional small molecules
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Charting bioethical frontiers:China’s human organoid guidelines in a global context 认领 引用 被引量：2
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作者 Liang-Bin Zhou Yi-Ting Lei Xin-Xin Han 《Military Medical Research》 SCIE CAS CSCD 2026年第2期340-344,共5页
The rapid progress of human organoid toolkits presents unprecedented opportunities in disease modeling,drug discovery,and personalized therapy,alongside profound bioethical challenges and concerns.On April 29th,2025,C... The rapid progress of human organoid toolkits presents unprecedented opportunities in disease modeling,drug discovery,and personalized therapy,alongside profound bioethical challenges and concerns.On April 29th,2025,China’s National Science and Technology Ethics Committee(Life Science Ethics Subcommittee)issued the Human Organoid Research Ethical Guidelines[1],establishing the world’s first comprehensive governance framework,especially focusing on brain organoids,embryo models,and chimeric research.This Guidelines represent a pioneering governance model in emerging biotechnology.This commentary offers an in-depth analysis to examine the policy’s innovative three-tiered structure,contrast it with current global regulatory standards,evaluate its domestic impacts as well as potential implications for international biotechnology governance,and provide recommendations for future directions. 展开更多
关键词 Human organoids Regulatory policy Ethical guidelines Brain organoids Embryo models Organoid chimeras
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Novel Small Molecule DZ-865B Effectively Degrades BCL6,Promotes Apoptosis and Reduces Proliferation of Diffuse Large B-Cell Lymphoma Cells 认领 引用
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作者 Yanfeng Wang Xinyi Chen +2 位作者 Yichen Yin Tao Li Jing Chen 《Oncology Research》 SCIE 2026年第3期602-621,共20页
Objectives:B-cell lymphoma 6(BCL6)is a transcriptional repressor whose overexpression is closely linked to the progression of diffuse large B-cell lymphoma(DLBCL),making it a promising therapeutic target.This study ai... Objectives:B-cell lymphoma 6(BCL6)is a transcriptional repressor whose overexpression is closely linked to the progression of diffuse large B-cell lymphoma(DLBCL),making it a promising therapeutic target.This study aims to identify a novel small molecule,synthesized via proteolysis-targeting chimeras(PROTACs),capable of degrading BCL6,thereby inhibiting DLBCL growth and providing a foundation for future preclinical studies.Methods:The expression of BCL6 in DLBCL was analyzed using The Cancer Genome Atlas(TCGA)database and the Human Protein Atlas.Western blotting assays confirmed BCL6 expression in tumor cell lines,leading to the identification of the small molecule compound DZ-865B.To evaluate DZ-865B’s in vitro efficacy,multiple assays were performed,including protein immunoblotting,immunofluorescence,reverse transcription quantitative PCR,EDU proliferation,and soft agar cloning assays.Results:TCGA analysis revealed significant overexpression of BCL6 in DLBCL(p<0.05),corroborated by immunohistological staining and western blotting.DZ-865B induced BCL6 degradation in DLBCL cell lines(OCI-LY-1 and SU-DHL-4)in a concentration-and time-dependent manner,and induced the degradation of nuclear BCL6 through the ubiquitin-proteasome pathway.Notably,DZ-865B did not alter BCL6 mRNA levels but modulated downstream gene expression,leading to the activation of apoptosis pathway proteins and inhibition of DNA synthesis,effectively suppressing DLBCL cell growth.Conclusion:This study demonstrates that the small molecule DZ-865B targets and degrades BCL6 in DLBCL cells,promoting apoptosis and inhibiting cellular proliferation.These findings highlight DZ-865B as a potential therapeutic agent for diffuse large B-cell lymphoma. 展开更多
关键词 Diffuse large B-cell lymphoma(DLBCL) B-cell lymphoma 6(BCL6) proteolysis-targeting chimeras(PROTACs) proliferation
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A Proximity-Triggered Strategy toward Transferable Proteolysis Targeting Chimeras 认领 引用 被引量：1
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作者 Yuena Wang Rongtong Zhao +14 位作者 Chuan Wan Wei Kang Rui Wang Chengyao Chiang Xiaochun Guo Qi Chang Zhanfeng Hou Yuxin Ye Qinhong Luo Ziyuan Zhou Jianbo Liu Shuiming Li Dongyuan Wang Feng Yin Zigang Li 《CCS Chemistry》 CSCD 2023年第6期1433-1442,共10页
Over the past 20 years,great efforts have been invested in developing site-specific approaches to protein modification to dissect protein functions directly and accurately.Here,we report a proximitytriggered group tra... Over the past 20 years,great efforts have been invested in developing site-specific approaches to protein modification to dissect protein functions directly and accurately.Here,we report a proximitytriggered group transfer strategy from a sulfonium warhead to a Cysteine(Cys)residue of the target protein.With a guiding ligand,cargoes could be transferred selectively from a sulfonium center onto the Cys residue in the vicinity of their binding interface.The successful thalidomide transfer of sulfonium 1-X could be applied intracellularly for epidermal growth factor receptor degradation,highlighting the potential of group transfer strategy as a suite of chemical biology studies,including cell imaging,protein profiling,and protein degradation by simply employing different transferrable groups. 展开更多
关键词 sulfonium protein covalent modification proteolysis targeting chimeras site-specific modification degradation
Fixed points as regulatory hubs in discrete memristive neural networks:An analysis of the FitzHugh–Nagumo model 认领 引用
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作者 Shaobo He Jiawei Xiao +1 位作者 Qilai Chen Huihai Wang 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第6期207-226,共20页
This study investigates the dynamics of discrete memristive FitzHugh–Nagumo(FHN)neural networks.We introduce a discrete memristor with hyperbolic tangent nonlinearity and incorporate it into neuron models ranging fro... This study investigates the dynamics of discrete memristive FitzHugh–Nagumo(FHN)neural networks.We introduce a discrete memristor with hyperbolic tangent nonlinearity and incorporate it into neuron models ranging from single neurons and coupled pairs to complex networks with ring and small-world topologies.Stability and bifurcation analyses reveal transitions from periodic to chaotic dynamics.A key contribution is the identification of a constant fixed point that remains invariant across periodic,weakly chaotic,and chaotic regimes.Linear stability analysis of this fixed point provides a fundamental basis for understanding the system's dynamical evolution.The fixed point theory explains how memristive coupling induces diverse synchronization patterns,including stable phase-locking and synchronization–desynchronization transitions,and further accounts for the emergence of chimera states in ring networks as well as their alteration in smallworld networks owing to long-range connections.Field-programmable gate array(FPGA)implementation successfully validates the mathematical models,confirming the feasibility of hardware realization.Overall,this work establishes a theoretical framework linking fixed point properties with firing mechanisms and synchronization dynamics in discrete memristive FHN neural networks,providing insights into potential applications in neuromorphic computing. 展开更多
关键词 discrete memristor discrete neuron complex network chimera state FPGA implementation
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Studies of substrate binding region of mEAAC1 and mASCT1 by constructing chimeras 认领 引用
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作者 Jing Li Jibin Peng +3 位作者 Jian Fei Fang Huang Quanbao Gu Lihe Guo 《Chinese Science Bulletin》 1999年第6期524-528,共5页
The cDNA chimeras between two subtypes of mouse excitatory amino acid transporter family, mouse excitatory amino acid carrier 1 （mEAAC1） and mouse alanine serine cysteine transporter 1 （mASCT1）, were constructed b... The cDNA chimeras between two subtypes of mouse excitatory amino acid transporter family, mouse excitatory amino acid carrier 1 （mEAAC1） and mouse alanine serine cysteine transporter 1 （mASCT1）, were constructed by recombinant PCR. After transcription in vitro, the cRNA was injected and expressed in Xenopus laevis oocytes. 3H-Glu and 3H-Ser were used as isotopic tracer to measure the flux of amino acids. The results showed that there might not be the key amino acids responsible for substractive specificity in the NH2-terminal and its adjacent regions of these two transporters, which probably supported the formation of the substrata binding sites. 展开更多
关键词 mEAAC1 mASCT1 chimeras substrate binding region.
Spiral wave chimeras in populations of oscillators coupled to a slowly varying diffusive environment 认领 引用
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作者 Lei Yang Yuan He Bing-Wei Li 《Frontiers of physics》 SCIE CSCD 2023年第1期71-83,共13页
Chimera states are firstly discovered in nonlocally coupled oscillator systems.Such a nonlocal coupling arises typically as oscillators are coupled via an external environment whose characteristic time scaleτis so sm... Chimera states are firstly discovered in nonlocally coupled oscillator systems.Such a nonlocal coupling arises typically as oscillators are coupled via an external environment whose characteristic time scaleτis so small(i.e.,τ→0)that it could be eliminated adiabatically.Nevertheless,whether the chimera states still exist in the opposite situation(i.e.,τ≫1)is unknown.Here,by coupling large populations of Stuart–Landau oscillators to a diffusive environment,we demonstrate that spiral wave chimeras do exist in this oscillator-environment coupling system even whenτis very large.Various transitions such as from spiral wave chimeras to spiral waves or unstable spiral wave chimeras as functions of the system parameters are explored.A physical picture for explaining the formation of spiral wave chimeras is also provided.The existence of spiral wave chimeras is further confirmed in ensembles of FitzHugh–Nagumo oscillators with the similar oscillator-environment coupling mechanism.Our results provide an affirmative answer to the observation of spiral wave chimeras in populations of oscillators mediated via a slowly changing environment and give important hints to generate chimera patterns in both laboratory and realistic chemical or biological systems. 展开更多
关键词 spiral wave chimeras reaction-diffusion systems oscillator-environment coupling pattern formation
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Research Progress in Induction Technique and Mechanism of Polyploid Lilium 认领 引用
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作者 Chen Mingxuan Wang Zhaowen Su Jingyan 《Journal of Northeast Agricultural University(English Edition)》 CAS 2026年第2期77-84,共8页
Polyploid breeding in Lilium is a pivotal biotechnology for generating novel germplasm,overcoming hybridization barriers and enhancing ornamental and economic value.This review systematically reviewed the latest resea... Polyploid breeding in Lilium is a pivotal biotechnology for generating novel germplasm,overcoming hybridization barriers and enhancing ornamental and economic value.This review systematically reviewed the latest research advances in lily polyploid induction,providing an in-depth analysis of two core technical pathways,somatic chromosome doubling and sexual polyploidization.The review compared the molecular mechanisms of traditional colchicine treatment with novel herbicides,such as oryzalin and trifluralin,and elaborated on strategies to optimize induction efficiency through explant selection and physico-chemical synergistic treatments.Simultaneously,the sexual polyploidization pathway was explored,analyzing the mechanisms of inducing unreduced gametes(2n gametes)via heat stress or N2O treatment and their application value in distant hybridization.To address the prevalent issue of chimera post-induction,this review outlined a tiered identification system ranging from morphological preliminary screening and precise flow cytometry detection to molecular cytogenetic confirmation.Furthermore,a chimera purification protocol based on in vitro regeneration cycles was proposed.Through comparative analysis,this review clarified the applicable scenarios and optimization directions for different technical solutions,aiming to provide a systematic reference for the rational design and efficient creation of lily polyploidy. 展开更多
关键词 Lilium polyploid induction flow cytometry chimera
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Entangled chimeras in nonlocally coupled bicomponent phase oscillators:From synchronous to asynchronous chimeras 认领 引用
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作者 Qiong-Lin Dai Xiao-Xuan Liu +4 位作者 Kai Yang Hong-Yan Cheng Hai-Hong Li Fagen Xie Jun-Zhong Yang 《Frontiers of physics》 SCIE CSCD 2020年第6期115-123,共9页
Chimera states,a symmetry-breaking spatiotemporal pattern in nonlocally coupled identical dynamical units,have been identified in various systems and generalized to coupled nonidentical oscillators.It has been shown t... Chimera states,a symmetry-breaking spatiotemporal pattern in nonlocally coupled identical dynamical units,have been identified in various systems and generalized to coupled nonidentical oscillators.It has been shown that strong heterogeneity in the frequencies of nonidentical oscillators might be harmful to chimera states.In this work,we consider a ring of nonlocally coupled bicomponent phase oscillators in which two types of oscillators are randomly distributed along the ring:some oscillators with natural.frequency w1 and others with w2.In this model,the heterogeneity in frequency is measured by frequency mismatch|w1-w2|between the oscillators in these two subpopulations.We report that the nonlocally coupled bicomponent phase oscillators allow for chimera states no matter how large the frequency mismatch is.The bicomponent oscillators are composed of two chimera states,one supported by oscillators with natural frequency wI and the other by oscillators with natural frequency w2.The two chimera states in two subpopulations are synchronized at weak frequency mismatch,in which the coberent oscillators in thern share similar mean phase velocity,and are desynchronized at large frequency mismatch,in which the coherent oscillators in different subpopulations have distinct mean phase velocities.The synchronization-desynchronization transition between chimera states in these two subpopulations is observed with the increase in the frequency mismatch.The observed phenomena are theoretically analyzed by passing to the continuum limit and using the Ott-Antonsen approach. 展开更多
关键词 chimera states bicomponent phase oscillators nonlocal coupling desynchronization transition
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Construction of serial deletants and chimeras of multi-kringle containing molecules and primary analysis of their functions 认领 引用
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作者 宫锋 董春娜 +3 位作者 张咏 章杨培 吴祖泽 贺福初 《Science China(Life Sciences)》 CAS 1999年第5期548-553,共6页
In comparison of amino acid sequences of 4 kringles of both macrophage stimulating protein (MSP) and hepatocyte growth factor (HGF), consensus motif sequence was determined. According to this consensus sequence, a pai... In comparison of amino acid sequences of 4 kringles of both macrophage stimulating protein (MSP) and hepatocyte growth factor (HGF), consensus motif sequence was determined. According to this consensus sequence, a pair of universal primers were designed. In combination with specific upstream or downstream primer of MSP or HGF respectively, serial fragments containing variant number of kringle (from 1 to 4) can be obtained by once PCR. By ligating the C terminal and N terminal fragments with different combination, serial deletants and chimeras of MSP and HGF were constructed. Sequence analysis showed that the degeneracy for universal primers and the sequences of those constructed deletants and chimeras are desired. Biological assay of these deletants revealed that wild type MSP can inhibit the growth of some tumor cell lines and that kringle 1 of MSP is essential for function as that of HGF. 展开更多
关键词 kringle HGF MSP deletant chimera.
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“Chimera+MolAICal”软件在药物化学教学中的应用——伊马替尼与蛋白酪氨酸激酶的分子对接 认领 引用 被引量：1
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作者 臧青民 章烨雯 +1 位作者 蒋德旗 吴桂卓 《化学教育（中英文）》 CAS 北大核心 2025年第4期99-107,共9页
探讨了Chimera和MolAICal软件在药物化学教学中的应用。传统药物化学教学往往受限于理论知识的抽象性和学生实践经验的匮乏,故难以有效地培养学生的理解能力和应用能力。为了解决这一问题,将Chimera与MolAICal软件相结合,应用于药物化... 探讨了Chimera和MolAICal软件在药物化学教学中的应用。传统药物化学教学往往受限于理论知识的抽象性和学生实践经验的匮乏,故难以有效地培养学生的理解能力和应用能力。为了解决这一问题,将Chimera与MolAICal软件相结合,应用于药物化学教学中。首先介绍了Chimera和MolAICal软件的基本功能和特点,然后通过设计抗癌药物伊马替尼与Bcr-Abl蛋白酪氨酸激酶的分子对接实验,学生们可以直观地观察到药物分子与靶蛋白的结合模式,增强了他们对分子间相互作用的理解。此外,学生通过自主选择最新的苗头化合物进行分子对接拓展实验,进一步加深了学生对药物设计和开发流程的感性认识和理性理解。将Chimera与MolAICal软件融入药物化学教学中,不仅丰富了教学手段,还显著提升了教学效果。 展开更多
关键词 MolAICal Chimera 伊马替尼 蛋白酪氨酸激酶 分子对接 药物化学教学
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