Cytoplasmic male sterility(CMS)is a widespread phenomenon in crops and is beneficial for commercial hybrid breeding.Although CMS systems in Brassica species are extensively utilized,they are derived from relatively li...Cytoplasmic male sterility(CMS)is a widespread phenomenon in crops and is beneficial for commercial hybrid breeding.Although CMS systems in Brassica species are extensively utilized,they are derived from relatively limited sources,and the underlying molecular mechanisms are not well understood.In this study,a novel CMS line,named Neau,was identified through distant hybridization between Matthiola incana and Brassica rapa.Neau CMS exhibits agronomic traits similar to its maintainer line of Chinese cabbage and displays sterility at the tetrad stage.The mitochondrial genomes of Neau CMS and its maintainer line were assembled to 359596 bp and 219771 bp,respectively,encoding 99 and 66 unknown open reading frames(orfs).Six orfs were identified as specific to Neau CMS with sequence alignments,with orf154 and two copies of orf138 identified as candidate genes based on their transmembrane structures and their ability to encode toxic proteins that inhibited Escherichia coli cell growth.The expression of orf154 was induced during the tetrad stage by quantitative real-time polymerase chain reaction(qRT-PCR),whereas orf138a showed higher expression at the microspore mononuclear stage in Neau CMS.Transcriptome analysis revealed reduced expressions of most pollen development-related genes,as well as genes involved in peroxidase/oxidative stress responses and the electron transfer chain in Neau CMS.Additionally,Neau CMS exhibited increased levels of O2.-,H2O2,and malondialdehyde(MDA),alongside reduced activities of SOD,CAT,and POD.Both ATP content and ATPase activity were down-regulated in Neau CMS.Overexpression of orf138 and orf154 in Arabidopsis thaliana resulted in reductions in pollen quantity,viability,and seed production.These findings suggest that orf138 and orf154 are key candidate genes responsible for the sterility observed in Neau CMS in Chinese cabbage.展开更多
为了更好地适配我国工业化特点和需求,推动产业升级和创新发展,设计并实现基于国产操作系统KylinOS的抓斗卸船机起重机管理系统(crane management system,CMS)架构。简要阐述抓斗卸船机CMS数据层与展现层的双层架构设计,探究程序开发过...为了更好地适配我国工业化特点和需求,推动产业升级和创新发展,设计并实现基于国产操作系统KylinOS的抓斗卸船机起重机管理系统(crane management system,CMS)架构。简要阐述抓斗卸船机CMS数据层与展现层的双层架构设计,探究程序开发过程控制的对象链接与嵌入(object linking and embedding for process control,OPC)软件通信、数据采集、CMS界面设计与功能实现。研究结果表明,基于国产操作系统重新研发设计的抓斗卸船机CMS架构可行性较高,该系统能正常运行且稳定可靠,可以解决技术依赖、数据安全、版权许可等问题,并为后续我国工业国产化升级提供参考。展开更多
Glucosinolates(GSLs),primarily found in Brassicaceae species,endow plants with functions in enhancing defense,providing therapeutic benefits,and contributing to unique food flavors.In the Ogu CMS restorer lines of rap...Glucosinolates(GSLs),primarily found in Brassicaceae species,endow plants with functions in enhancing defense,providing therapeutic benefits,and contributing to unique food flavors.In the Ogu CMS restorer lines of rapeseed(Brassica napus L.),the introduction of extra radish genome segments contributes to the elevated accumulation of aliphatic glucosinolates(AGSLs)in leaves and seeds.However,the molecular mechanisms underlying AGSL biosynthesis in these restorer lines remain unclear.In this study,the expression profile of genes involved in the glucosinolate(GSL)biosynthesis pathway,were characterized revealing that GSL biosynthesis is most active in the silique pericarps.Through sequence alignment and functional analysis,a foreign RsMYB28 allele was identified and proposed as the key regulator of AGSL biosynthesis in Ogu CMS restorer lines.Functional validation via RsMYB28 overexpression confirmed its role in promoting AGSL accumulation.RsMYB28 specifically bound to the promoters of BnaIMDH3 and BnaCYP83A1,and activated their transcription.This study elucidates the core function of RsMYB28 in regulating AGSL biosynthesis in Ogu CMS restorer lines,providing a valuable genetic resource for improving rapeseed resistance and quality through breeding.展开更多
基金funded by National Natural Science Foundation of China(Grant No.32202483)the Foundation for Key Research and Development Program of Heilongjiang Province(Grant No.SC2022ZX02C0202).
摘要Cytoplasmic male sterility(CMS)is a widespread phenomenon in crops and is beneficial for commercial hybrid breeding.Although CMS systems in Brassica species are extensively utilized,they are derived from relatively limited sources,and the underlying molecular mechanisms are not well understood.In this study,a novel CMS line,named Neau,was identified through distant hybridization between Matthiola incana and Brassica rapa.Neau CMS exhibits agronomic traits similar to its maintainer line of Chinese cabbage and displays sterility at the tetrad stage.The mitochondrial genomes of Neau CMS and its maintainer line were assembled to 359596 bp and 219771 bp,respectively,encoding 99 and 66 unknown open reading frames(orfs).Six orfs were identified as specific to Neau CMS with sequence alignments,with orf154 and two copies of orf138 identified as candidate genes based on their transmembrane structures and their ability to encode toxic proteins that inhibited Escherichia coli cell growth.The expression of orf154 was induced during the tetrad stage by quantitative real-time polymerase chain reaction(qRT-PCR),whereas orf138a showed higher expression at the microspore mononuclear stage in Neau CMS.Transcriptome analysis revealed reduced expressions of most pollen development-related genes,as well as genes involved in peroxidase/oxidative stress responses and the electron transfer chain in Neau CMS.Additionally,Neau CMS exhibited increased levels of O2.-,H2O2,and malondialdehyde(MDA),alongside reduced activities of SOD,CAT,and POD.Both ATP content and ATPase activity were down-regulated in Neau CMS.Overexpression of orf138 and orf154 in Arabidopsis thaliana resulted in reductions in pollen quantity,viability,and seed production.These findings suggest that orf138 and orf154 are key candidate genes responsible for the sterility observed in Neau CMS in Chinese cabbage.
摘要为了更好地适配我国工业化特点和需求,推动产业升级和创新发展,设计并实现基于国产操作系统KylinOS的抓斗卸船机起重机管理系统(crane management system,CMS)架构。简要阐述抓斗卸船机CMS数据层与展现层的双层架构设计,探究程序开发过程控制的对象链接与嵌入(object linking and embedding for process control,OPC)软件通信、数据采集、CMS界面设计与功能实现。研究结果表明,基于国产操作系统重新研发设计的抓斗卸船机CMS架构可行性较高,该系统能正常运行且稳定可靠,可以解决技术依赖、数据安全、版权许可等问题,并为后续我国工业国产化升级提供参考。
基金supported by the National Key Research and Development Program of China(2023YFD1201403)the Natural Science Foundation of Hunan Province(2025JJ60173)+4 种基金the Science and Technology Innovation Program of Hunan Province(2023RC1077)the Hunan Provincial Science and Technology Talent Promotion Project(2023TJ-Z09)the Hunan Agricultural Sci-Tech Innovation Funding Project(2024CX032)the Yuelushan Laboratory Talent Program(2024RC2071)the Yuelushan Laboratory Breeding Program(YLS-2025-ZY02016).
摘要Glucosinolates(GSLs),primarily found in Brassicaceae species,endow plants with functions in enhancing defense,providing therapeutic benefits,and contributing to unique food flavors.In the Ogu CMS restorer lines of rapeseed(Brassica napus L.),the introduction of extra radish genome segments contributes to the elevated accumulation of aliphatic glucosinolates(AGSLs)in leaves and seeds.However,the molecular mechanisms underlying AGSL biosynthesis in these restorer lines remain unclear.In this study,the expression profile of genes involved in the glucosinolate(GSL)biosynthesis pathway,were characterized revealing that GSL biosynthesis is most active in the silique pericarps.Through sequence alignment and functional analysis,a foreign RsMYB28 allele was identified and proposed as the key regulator of AGSL biosynthesis in Ogu CMS restorer lines.Functional validation via RsMYB28 overexpression confirmed its role in promoting AGSL accumulation.RsMYB28 specifically bound to the promoters of BnaIMDH3 and BnaCYP83A1,and activated their transcription.This study elucidates the core function of RsMYB28 in regulating AGSL biosynthesis in Ogu CMS restorer lines,providing a valuable genetic resource for improving rapeseed resistance and quality through breeding.