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Distribution and Transmission of Apramycin-Resistant Escherichia coli from Humans and Animal-Producing Sectors:A Multicenter,Cross-Sectional,and One Health Study 认领 引用
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作者 Yue Cao Dejun Liu +23 位作者 Fen Pan Zhenzhen Liu Qin Zhang Chengtao Sun Li Ding Siquan Shen Weishuai Zhai Rina Bai Zhiyu Zou Yiqing Wang Lu Yang Zexun Lv Bo Fu Shizhen Ma Yao Wang Ke Zhao Tingxuan Shi Yingbo Shen Rong Zhang Timothy R.Walsh Jianzhong Shen Fupin Hu Yang Wang Congming Wu 《Engineering》 SCIE EI CSCD 2026年第5期116-126,共11页
Apramycin,an aminoglycoside antibiotic used exclusively in veterinary medicine,has attracted growing interest for its potential clinical application owing to its low toxicity and potent activity against multidrug-resi... Apramycin,an aminoglycoside antibiotic used exclusively in veterinary medicine,has attracted growing interest for its potential clinical application owing to its low toxicity and potent activity against multidrug-resistant(MDR)bacteria.Despite the completion of two Phase I clinical trials,apramycin resistance dynamics across One Health interfaces remain poorly understood.This study,conducted from 2020to 2023 in Chengdu,Qingdao,and Shanghai,China,collected 5160 non-duplicate samples from hospitals,broiler and pig farms and slaughterhouses,and markets.We identified 1394 isolates of apramycinresistant Escherichia coli(E.coli)(AREC),with the highest detection rates in animal feces(58%,700/1214),followed by animal carcasses(47%,183/393),fresh meat(35%,229/659),environments(21%,127/593),human feces(7%,103/1425),and clinical samples(5%,42/876).Detection rates were higher in broiler-producing chains(57%,742/1292)than in pig-producing chains(32%,512/1609).Most AREC isolates(99.7%,1390/1394)carried the aac(3)-Ⅳgene,conferring resistance to apramycin,gentamicin,and tobramycin.Genomic analysis of 742 AREC isolates revealed sporadic clonal transmission events between animals and humans in Qingdao and Shanghai.Long-read sequencing of 66 representative isolates showed that aac(3)-Ⅳgenes were primarily located on IncHI2/IncHI2A plasmids,with high structural conservation across different sources.Temporal surveillance indicated a sharp increase in aac(3)-Ⅳprevalence in livestock-associated E.coli following the adoption of apramycin in China.These findings demonstrate the rapid,plasmid-driven dissemination of apramycin resistance at the One Health interface,underscore the need for prudent veterinary stewardship and careful consideration of apramycin's clinical repurposing for human use. 展开更多
关键词 Apramycin resistance Escherichia coli One Health IncHI2/IncHI2A plasmids aac(3)-IV
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A colorimetric biosensor for Escherichia coli detection in food based on aptamer-enhanced oxidase-mimicking activity of octahedral Ag2O nanoparticles 认领 引用
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作者 Xiaohuan Huang Ling Zhang +6 位作者 Tingshu Lu Jie Li Mian Wang Wei Dai Chengyin Zhou Jia Xu Yuangen Wu 《Food Innovation and Advances》 2026年第2期185-194,共10页
Escherichia coli(E.coli)poses a grave threat to food safety,underscoring the need for expeditious and precise detection methodologies.Conventional colorimetric approaches are simple,but they frequently lack accuracy a... Escherichia coli(E.coli)poses a grave threat to food safety,underscoring the need for expeditious and precise detection methodologies.Conventional colorimetric approaches are simple,but they frequently lack accuracy and stability.This study constructed an aptamer-based colorimetric biosensor,leveraging the enzyme-mimicking activity of octahedral Ag2O nanoparticles(NPs),mediated by oligonucleotides,aiming for highly sensitive foodborne E.coli detection.The P12-55 aptamer can be adsorbed onto the octahedral Ag2O NPs surface,thereby significantly enhancing their oxidase-mimicking activity.The aptamer enhances activity by promoting·O2−generation,and accelerating electron transfer to the 3,3',5,5'-tetramethylbenzidine(TMB)substrate.In the presence of E.coli in the sensing system,the aptamer exhibits a preferential binding affinity for the bacteria,thereby restoring the oxidase-mimicking activity and enabling the transduction of the detection signal.The biosensor exhibited a range of linear detection of 3×102-3×108CFU·mL−1,and the limit of detection(LOD)was as low as 4 CFU·mL−1.Recovery rates of 99.1%to 104%were achieved in milk and tap water samples,demonstrating outstanding practicality and reliability.This study proposes a novel and efficient method for the rapid detection of harmful bacteria in food,which is of significant importance for food safety assurance. 展开更多
关键词 detection methodologiesconventional Escherichia coli detection colorimetric approaches octahedral Ag O nanoparticles colorimetric biosensorleveraging oxidase mimicking activity colorimetric biosensor food safety
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Impact of dietary supplementation of Bacillus subtilis on the metabolic profiles and microbial ecology of weanling pigs experimentally infected with a pathogenic Escherichia coli 认领 引用
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作者 Kwangwook Kim Sangwoo Park +2 位作者 Cynthia Jinno Peng Ji Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期1020-1033,共14页
Background Our previous study demonstrated that dietary supplementation of Bacillus subtilis enhanced growth performance and intestinal integrity in weaned pigs challenged with enterotoxigenic Escherichia coli(ETEC).T... Background Our previous study demonstrated that dietary supplementation of Bacillus subtilis enhanced growth performance and intestinal integrity in weaned pigs challenged with enterotoxigenic Escherichia coli(ETEC).Therefore,this study aimed to explore the impact of Bacillus subtilis on gut health and its role in modulating host–microbe interactions in post-weaning pigs.Results ETEC infection disrupted key metabolic pathways in distal colon,including glutathione,beta-alanine,and pyrimidine metabolism,indicating increased oxidative stress,impaired nucleotide balance,and amino acid catabolic stress.Bacillus subtilis supplementation induced distinct metabolomic and microbiome profiles in colon digesta of weaned pigs challenged with ETEC.Bacillus subtilis-treated pigs under ETEC challenge exhibited significant enrichment in amino acid-and energy-related pathways such as arginine biosynthesis,phenylalanine metabolism,pantothenate and CoA biosynthesis.ETEC infection induced microbial dysbiosis in the distal colon,resulting in decrease(P<0.05)in abundance of Streptococcaceae and Enterobacteriaceae compared to healthy controls.Bacillus subtilis supplementation mitigated the ETEC-induced disruptions by increasing the relative abundance of beneficial bacterial families,including Lachnospiraceae and Bacteroidaceae.Conclusion Supplementation of Bacillus subtilis improves intestinal health and resilience against ETEC challenge by mitigating infection-induced metabolic disruptions and gut dysbiosis in weaned pigs. 展开更多
关键词 Bacillus subtilis Colon microbiota Enterotoxigenic Escherichia coli F18 Metabolomics Weaned pigs
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A microfluidic biosensor based on multiple signal amplification for rapid and sensitive detection of E.coli O157:H7 and Staphylococcus aureus 认领 引用
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作者 Xinrui Zhang Hui Chen +3 位作者 Tingting Fan Xueming Zhu Ying Tan Dan Gao 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第4期694-699,共6页
E.coli O157:H7 and Staphylococcus aureus have emerged as significant foodborne pathogens,characterized by considerable incidence rates and mortality.Despite advancements,current detection methods are hindered by chall... E.coli O157:H7 and Staphylococcus aureus have emerged as significant foodborne pathogens,characterized by considerable incidence rates and mortality.Despite advancements,current detection methods are hindered by challenges in enhancing specificity and sensitivity.Herein,we introduced a cutting-edge biosensor that employs a novel CHA-coupled CRISPR multi-stage signal amplification technique for the rapid and ultra-sensitive detection of these two pathogens.This microfluidic device consisted of an upstream serpentine mixing channel and a downstream boat-shaped microcavity equipped with a microcolumn array,facilitating efficient reagent mixing,robust CHA amplification,and CRISPR reactions.Multiple signal amplification was achieved through bacterial competitive binding triggered by catalytic hairpin assembly(CHA)and cr RNA-mediated CRISPR reactions.Based on this platform,the detection of target bacteria is transformed into nucleic acid detection,with a maximum detection range of 134 CFU/mL for E.coli O157:H7 and 181 CFU/mL for Staphylococcus aureus,which were better or comparable to previously reported biosensors.The entire assay was completed within approximately 1.5 h,with a minimal sample volume requirement of just 10μL.The biosensor exhibited a high recovery rate,ranging from 95%to 115%,and demonstrated excellent specificity towards the target bacteria.In summary,this biosensor offers a rapid,accurate,and highly sensitive tool for food safety and clinical diagnostics. 展开更多
关键词 Microfluidic chip Catalytic hairpin assembly CRISPR Staphylococcus aureus E.coli O157:H7
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A metabolic optimization strategy based on modular genomic integration of the MVA pathway to enhance lycopene production in Escherichia coli 认领 引用
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作者 Wanpeng Xia Wenqian Li +1 位作者 Qiang Yao Jingyu Chen 《Food Innovation and Advances》 2026年第2期251-260,共10页
Lycopene,a bioactive tetraterpenoid antioxidant valued in food,pharmaceutical,and cosmetic sectors,remains constrained by low plant extractability and expensive chemical synthesis;microbial engineering now presents a ... Lycopene,a bioactive tetraterpenoid antioxidant valued in food,pharmaceutical,and cosmetic sectors,remains constrained by low plant extractability and expensive chemical synthesis;microbial engineering now presents a cost-effective alternative.In this study,key genes from the methylerythritol phosphate(MEP)pathway—dxs,idi,and ispDF—were integrated into the genome of the chassis strain Escherichia coli MG1655 using a CRISPR-Cpf1-based system,resulting in MEP pathway-overexpressed strains.Additionally,the downstream module(MBot)of the mevalonate(MVA)pathway was optimized by introducing T7 RNA polymerase,mvaE,and mvaS from different species,including Saccharomyces cerevisiae,Streptococcus pneumoniae,and Staphylococcus aureus.Integration of the MEP genes improved lycopene production by 2 mg/L compared with the initial strain.Notably,fermentation performance varied significantly depending on the source of the downstream MBot module.The optimal combination—erg12 from Saccharomyces cerevisiae,mvaK and mvd1 from Streptococcus pneumoniae,and idi from Escherichia coli—achieved a lycopene titer of 86 mg/L in shake-flask cultures,representing a 21-fold increase compared to the parental strain.Paradoxically,dxr deletion to eliminate endogenous MEP flux precipitated a 7-fold drop in lycopene titre,whereas MEP overexpression failed to enhance production—revealing that MEP–MVA pathway synergy,rather than simple precursor supply,governs efficient carotenoid biosynthesis. 展开更多
关键词 lycopene production mva pathway methylerythritol phosphate mep pathway dxsidiand integrated genome metabolic optimization modular genomic integration chemical synthesismicrobial engineering escherichia coli mg
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Detection of intracellular bacterial communities and biofilms in urinary tract infections with Escherichia coli using staining protocols 认领 引用
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作者 Swathi Pandey Arul Senghor Kadalangudi Aravaanan +1 位作者 Emmanuel Bhaskar Santhi Silambanan 《World Journal of Experimental Medicine》 2026年第2期48-60,共13页
BACKGROUND Urinary tract infections(UTIs)are prevalent worldwide,and Escherichia coli(E.coli)is the most common causative agent.The ability of the bacteria to form intracellular bacterial communities(IBCs)and biofilm ... BACKGROUND Urinary tract infections(UTIs)are prevalent worldwide,and Escherichia coli(E.coli)is the most common causative agent.The ability of the bacteria to form intracellular bacterial communities(IBCs)and biofilm is a major reason for UTIs.Studies have indicated that the persistence of uropathogenic E.coli as IBCs and biofilms has been implicated in UTIs.However,IBCs are not routinely identified by standard diagnostic methods.AIM To compare the various staining techniques for the detection of IBCs in urine samples from E.coli culture-positive UTI patients with the biofilm-forming capability of the isolates.METHODS The study included 73 patients with E.coli culture-confirmed UTI.Before antibiotic treatment midstream urine sample was collected,and the sediment was obtained by centrifugation.The samples were visualized using Sternheimer-Malbin,Wright-Giemsa,Safranin,and immunofluorescence staining to detect IBCs.Formation of biofilms was analyzed by the tube method.Descriptive statistics were used.RESULTS E.coli clusters were seen by light microscopy using various stains.However,immunofluorescence staining showed a better picture in the form of bright intracellular signals,which indicate bacterial aggregates.Biofilm assay showed an association with intracellular colonization.CONCLUSION The various staining techniques help in the identification of uropathogenic E.coli as IBCs inside superficial epithelial cells.These bacteria are also capable of forming biofilms,which resists action of antibiotics.Thus,IBCs and biofilms are rich reservoirs of organisms in the urinary bladder,paving the way for chronic treatment-resistant UTIs.This study requires further larger studies to substantiate these findings. 展开更多
关键词 Urinary tract infections Intracellular bacterial communities Biofilm formation Sustainable Development Goals-3 Escherichia coli
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Multidrug-resistant Escherichia coli:Updated insights into resistance mechanisms,rapid diagnostics,and emerging control strategies 认领 引用
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作者 Asmaa S.Mansour 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2026年第9期363-375,共13页
Multidrug-resistant(MDR)Escherichia coli(E.coli)is a major global health threat,causing increased morbidity,mortality,and healthcare burden.It is a common cause of community-and hospital-acquired infections,with a str... Multidrug-resistant(MDR)Escherichia coli(E.coli)is a major global health threat,causing increased morbidity,mortality,and healthcare burden.It is a common cause of community-and hospital-acquired infections,with a strong ability to acquire and disseminate resistance genes via horizontal gene transfer and mutations.This review provides an overview of MDR E.coli,focusing on resistance mechanisms,diagnostic approaches,and control strategies.Key resistance mechanisms include production of extended-spectrumβ-lactamases(ESBLs),carbapenemases,efflux pump overexpression,target site alterations,and reduced membrane permeability.Quorum sensing and biofilm formation further enhance bacterial survival and resistance.Diagnostic methods range from conventional antimicrobial susceptibility testing to advanced molecular and genomic techniques such as PCR and whole-genome sequencing.Emerging tools,including Fourier transform infrared spectroscopy,surface-enhanced Raman spectroscopy,MALDI-TOF MS,automated systems,and biosensors,offer rapid and accurate detection.Control strategies involve antimicrobial stewardship programs,alternative therapies such as natural compounds and QS inhibitors,and strict infection prevention measures.Addressing MDR E.coli requires a multidisciplinary approach integrating timely diagnosis,effective treatment,and robust infection control policies.A comprehensive literature search was conducted across major databases,and selected studies were qualitatively analyzed to summarize current knowledge and highlight emerging trends.Understanding these aspects is essential for limiting the spread of MDR E.coli and improving clinical outcomes globally. 展开更多
关键词 Multidrug-resistant Escherichia coli Antimicrobial resistance Resistance mechanisms Diagnostic techniques Control approaches
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Dietary iron and metal-based growth promoters differentially modulate the gut resistome and Escherichia coli virulome in weaned pigs 认领 引用
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作者 Shya E.Navazesh Anneliek ter Horst +6 位作者 Weizhang Wen Yanhong Liu David Kiang Zhirong Li Alice Yu C.Titus Brown Peng Ji 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第4期2337-2353,共17页
Background High levels of zinc oxide(ZnO)and copper sulfate are widely used as alternative growth promoters in postweaning pig diet.However,excessive exposure to these metals may drive co-selection for heavy metal(HMR... Background High levels of zinc oxide(ZnO)and copper sulfate are widely used as alternative growth promoters in postweaning pig diet.However,excessive exposure to these metals may drive co-selection for heavy metal(HMR)and antibiotic resistance(AMR).Nursery diets also contain abundant iron to offset the low bioavailability of plantderived iron,yet how dietary iron influence gut dysbiosis and microbial resistance in postweaning pigs remains unclear.This exploratory study examined the effects of dietary iron and metal-based growth promoters on the fecal resistome of postweaning pigs using shotgun metagenomics and whole-genome sequencing(WGS).Methods Fifty weanling pigs were stratified and randomly assigned to five dietary treatments for 24 d.Experimental diets included a control diet(Con)containing 25,139,and 141 mg/kg of Cu,Fe,and Zn,respectively,a low-iron diet(LFe,19 mg Fe/kg),a high-iron diet(HFe,1,219 mg Fe/kg),a high-copper diet(HCu,257 mg Cu/kg),and a high-zinc diet(HZn,2,631 mg Zn/kg,including 2,490 mg Zn/kg from ZnO).All pigs were orally administered with F18 enterotoxigenic Escherichia coli(ETEC)on d 13–16.Metagenome sequencing were performed on d 24 fecal DNA(n=24)to identify HMR genes(BacMet Predicted database)and AMR genes(CARD database).Functional annotation was performed using HUMAnN3.Whole genome sequencing(WGS)was conducted on 120 E.coli isolates from fecal cultures on d 1,12,and 24,and AMR and virulence genes were identified from contig assemblies using ABRicate.Results Dietary metal treatments significantly alteredβ-diversity of HMR genes compared with Con,with HZn differing from both HCu and LFe(P<0.05).Fecal iron levels correlated with sodB(ρ=0.64,P=0.075),an iron-containing superoxide dismutase,while fecal copper levels correlated with pcoC(ρ=0.66,P=0.075),a plasmid-mediated copper resistance gene.Across metagenomes,172 AMR genes were identified,dominated by glycopeptide and tetracycline resistance.While dietary iron had minimal effects on fecal AMR profile,HZn induced the largest shifts in resistome,including increases of ant(9)-la,conferring aminoglycoside resistance on mobile genetic elements,and adeF,encoding a multidrug efflux pump(P<0.05).Functional profiling revealed enrichment of carbohydrate metabolism pathways in HZn group(P<0.05).WGS of E.coli isolates showed distinct AMR profiles under HZn on d 24 and distinct virulence profile under LFe on d 12,exhibiting increased prevalence of exotoxin and T3SS genes(P<0.05).Conclusion Dietary iron restriction enhanced E.coli virulence genes,whereas excessive ZnO induced the most pronounced changes in the gut resistome and microbial metabolism,highlighting a risk for AMR co-selection and marked influence on gut microbiota. 展开更多
关键词 Antimicrobial resistance Dietary iron supplementation Enterotoxigenic E.coli Growth promoter Heavy metal resistance Pharmacological zinc oxide Postweaning pig
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Effects of dietary Lactobacillus postbiotics and bacitracin on the modulation of mucosaassociated microbiota and pattern recognition receptors affecting immunocompetence of jejunal mucosa in pigs challenged with enterotoxigenic F18+Escherichia coli 认领 引用 被引量：5
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作者 Marcos Elias Duarte Zixiao Deng Sung Woo Kim 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期282-299,共18页
Background Enterotoxigenic Escherichia coli(E.coli)is a threat to humans and animals that causes intestinal dis-orders.Antimicrobial resistance has urged alternatives,including Lactobacillus postbiotics,to mitigate th... Background Enterotoxigenic Escherichia coli(E.coli)is a threat to humans and animals that causes intestinal dis-orders.Antimicrobial resistance has urged alternatives,including Lactobacillus postbiotics,to mitigate the effects of enterotoxigenic E.coli.Methods Forty-eight newly weaned pigs were allotted to NC:no challengeo supplement;PC:F18+E.coli chal-lengeo supplement;ATB:F18+E.coli challenge/bacitracin;and LPB:F18+E.coli challenge/postbiotics and fed diets for 28 d.On d 7,pigs were orally inoculated withF18+E.coli.At d 28,the mucosa-associated microbiota,immune and oxidative stress status,intestinal morphology,the gene expression of pattern recognition receptors(PRR),and intestinal barrier function were measured.Data were analyzed using the MIXED procedure in SAS 9.4.Results PC increased(P<0.05)Helicobacter mastomyrinus whereas reduced(P<0.05)Prevotella copri and P.ster-corea compared to NC.The LPB increased(P<0.05)P.stercorea and Dialister succinatiphilus compared with PC.The ATB increased(P<0.05)Propionibacterium acnes,Corynebacterium glutamicum,and Sphingomonas pseudosanguinis compared to PC.The PC tended to reduce(P=0.054)PGLYRP4 and increased(P<0.05)TLR4,CD14,MDA,and crypt cell proliferation compared with NC.The ATB reduced(P<0.05)NOD1 compared with PC.The LPB increased(P<0.05)PGLYRP4,and interferon-γand reduced(P<0.05)NOD1 compared with PC.The ATB and LPB reduced(P<0.05)TNF-αand MDA compared with PC.Conclusions TheF18+E.coli challenge compromised intestinal health.Bacitracin increased beneficial bacteria show-ing a trend towards increasing the intestinal barrier function,possibly by reducing the expression of PRR genes.Lac-tobacillus postbiotics enhanced the immunocompetence of nursery pigs by increasing the expression of interferon-γand PGLYRP4,and by reducing TLR4,NOD1,and CD14. 展开更多
关键词 Escherichia coli Immunocompetence Intestinal health Pattern recognition receptors Pigs
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Advances and mechanisms of traditional Chinese medicine and its active ingredients against antibiotic-resistant Escherichia coli infections 认领 引用 被引量：3
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作者 Shuo Yang Ping Su +2 位作者 Lu Li Shuang Liu Yi Wang 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2025年第2期375-388,共14页
In clinical practice,antibiotics have historically been utilized for the treatment of pathogenic bacteria.However,the gradual emergence of antibiotic resistance among bacterial strains has posed a significant challeng... In clinical practice,antibiotics have historically been utilized for the treatment of pathogenic bacteria.However,the gradual emergence of antibiotic resistance among bacterial strains has posed a significant challenge to this approach.In 2022,Escherichia coli,a Gram-negative bacterium renowned for its widespread pathogenicity and high virulence,emerged as the predominant pathogenic bacterium in China.The rapid emergence of antibiotic-resistant E.coli strains has rendered antibiotics insufficient to fight E.coli infections.Traditional Chinese medicine(TCM)has made remarkable contributions to the health of Chinese people for thousands of years,and its significant therapeutic effects have been proven in clinical practice.In this paper,we provide a comprehensive review of the advances and mechanisms of TCM and its active ingredients against antibiotic-resistant E.coli infections.First of all,this review introduces the classification,antibiotic resistance characteristics and mechanisms of E.coli.Then,the TCM formulas and extracts are listed along with their active ingredients against E.coli,including extraction solution,minimum inhibitory concentration(MIC),and the antibacterial mechanisms.In addition,there is growing evidence supporting the synergistic therapeutic strategy of combining TCM with antibiotics for the treatment of antibiotic-resistant E.coli infections,and we provide a summary of this evidence and its underlying mechanisms.In conclusion,we present a comprehensive review of TCM and highlight its potential and advantages in the prevention and treatment of E.coli infections.We hold the opinion that TCM will play an important role in global health,pharmaceutical development,and livestock farming in the future. 展开更多
关键词 Escherichia coli Antibiotic-resistance Traditional Chinese medicine Active ingredients Antibacterial mechanism
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Characterization and genomics analysis of phage PGX1 against multidrug-resistant enterotoxigenic E.coli with in vivo and in vitro efficacy assessment 认领 引用 被引量：1
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作者 Dayue Hu Ping Qian +5 位作者 Dongyang Gao Xinxin Li Linkang Wang Hongyue Ji Shuang Wang Xiangmin Li 《Animal Diseases》 CAS CSCD 2025年第1期85-100,共16页
Enterotoxigenic E.coli is one of the bacterial pathogens contributing to the global resistance crisis in public health and animal husbandry.The problem of antibiotic resistance is becoming more and more serious,and ph... Enterotoxigenic E.coli is one of the bacterial pathogens contributing to the global resistance crisis in public health and animal husbandry.The problem of antibiotic resistance is becoming more and more serious,and phage is con-sidered one of the potential alternatives to antibiotics that could be utilized to treat bacterial infections.Our study isolated and identified a lytic phage PGX1 against multidrug-resistant enterotoxigenic E.coli EC6 strain from sew-age.The phage lysis profile revealed that PGX1 exhibited a lytic effect on multidrug-resistant enterotoxigenic E.coli strains of serotype O60.Through phage whole genome sequencing and bioinformatics analysis,PGX1 was found to be the class Caudoviricetes,family Autographiviridae,genus Teseptimavirus.The length of the PGX1 genome is about 37,009 bp,containing 54 open reading frames(ORFs).Notably,phage PGX1 lacks any lysogenic-related genes or virulence genes.Furthermore,phage PGX1 demonstrates strong adaptability,tolerance,and stability in various pH(pH4-10)and temperatures(4–40°C).The in vivo and in vitro tests demonstrated that phage PGX1 significantly removes and inhibits the formation of multidrug-resistant EC6 biofilm and effectively controls the Galleria mel-lonella larvae and enterotoxigenic E.coli EC6 during mice infection.In conclusion,the above findings demonstrated that phage PGX1 may be a novel antimicrobial agent to control multidrug-resistant E.coli infections. 展开更多
关键词 Enterotoxigenic E.coli Multidrug-resistant bacteria Phage PGX1
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Escherichia coli O157:H7的表面增强拉曼光谱检测方法建立 认领 引用
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作者 王盼雪 李岑 +2 位作者 李香 刘莹 李国梁 《食品与生物技术学报》 CAS CSCD 北大核心 2025年第6期97-105,共9页
【目的】提高大肠杆菌(Escherichia coli,E.coli)O157:H7检测的特异性和灵敏度。【方法】基于磁分离和信号放大策略,结合表面增强拉曼光谱(SERS)技术建立了一种特异性好、灵敏度高的E.coli O157:H7检测方法。以适配体为识别元件,联合磁... 【目的】提高大肠杆菌(Escherichia coli,E.coli)O157:H7检测的特异性和灵敏度。【方法】基于磁分离和信号放大策略,结合表面增强拉曼光谱(SERS)技术建立了一种特异性好、灵敏度高的E.coli O157:H7检测方法。以适配体为识别元件,联合磁珠和聚苯乙烯(PS)微球,分别构建分离探针MB@Apt和信号放大型拉曼标签4-MBN@PS@Apt。【结果】当E.coli O157:H7存在时,MB@Apt、4-MBN@PS@Apt与E.coli O157:H7可形成MB@Apt/E.coli O157:H7/4-MBN@PS@Apt“三明治”结构复合物,该复合物可借助外源磁场实现快速分离和富集;加入四氢呋喃(THF)后,拉曼标签上负载的低生物背景拉曼报告物4-MBN可被快速释放。4-MBN在1073.9 cm-1处的相对拉曼强度与E.coli O157:H7浓度的对数值在1×103~1×108 CFU/mL的线性关系良好,决定系数(R2)为0.98。该方法对E.coli O157:H7的检测限(LOD)为312 CFU/mL,在自来水和牛奶样本中的检测回收率为61.87%~135.55%,相对标准偏差(RSD)为0.27%~5.28%。【结论】该方法对E.coli O157:H7的检测灵敏度较高,为食源性致病菌的快速检测提供了新思路。 展开更多
关键词 Escherichia coli O157:H7 快速检测 表面增强拉曼光谱 聚苯乙烯微球 低生物背景
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Metagenomics reveals an increased proportion of an Escherichia colidominated enterotype in elderly Chinese people 认领 引用
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作者 Jinyou LI Yue WU +10 位作者 Yichen YANG Lufang CHEN Caihong HE Shixian ZHOU Shunmei HUANG Xia ZHANG Yuming WANG Qifeng GUI Haifeng LU Qin ZHANG Yunmei YANG 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2025年第5期477-492,共16页
Gut microbial communities are likely remodeled in tandem with accumulated physiological decline during aging,yet there is limited understanding of gut microbiome variation in advanced age.Here,we performed a metagenom... Gut microbial communities are likely remodeled in tandem with accumulated physiological decline during aging,yet there is limited understanding of gut microbiome variation in advanced age.Here,we performed a metagenomics-based enterotype analysis in a geographically homogeneous cohort of 367 enrolled Chinese individuals between the ages of 60 and 94 years,with the goal of characterizing the gut microbiome of elderly individuals and identifying factors linked to enterotype variations.In addition to two adult-like enterotypes dominated by Bacteroides(ET-Bacteroides)and Prevotella(ET-Prevotella),we identified a novel enterotype dominated by Escherichia(ET-Escherichia),whose prevalence increased in advanced age.Our data demonstrated that age explained more of the variance in the gut microbiome than previously identified factors such as type 2 diabetes mellitus(T2DM)or diet.We characterized the distinct taxonomic and functional profiles of ET-Escherichia,and found the strongest cohesion and highest robustness of the microbial co-occurrence network in this enterotype,as well as the lowest species diversity.In addition,we carried out a series of correlation analyses and co-abundance network analyses,which showed that several factors were likely linked to the overabundance of Escherichia members,including advanced age,vegetable intake,and fruit intake.Overall,our data revealed an enterotype variation characterized by Escherichia enrichment in the elderly population.Considering the different age distribution of each enterotype,these findings provide new insights into the changes that occur in the gut microbiome with age and highlight the importance of microbiome-based stratification of elderly individuals. 展开更多
关键词 Gut microbiome Aging Elderly population Escherichia-dominated enterotype Enterotype Escherichia coli
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Evaluation of antimicrobial resistance and virulence characteristics in Escherichia coli and Proteus mirabilis isolates from dead Crested Ibis embryos at the artificial breeding base 认领 引用
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作者 Gang Shu Lu Qu +9 位作者 Weiyi Yang Ziqin Lin Hanqian Dong Danqin Li Haiqiong Yang Wei Zhang Haohuan Li Funeng Xu Hualin Fu Juchun Lin 《Avian Research》 SCIE CAS CSCD 2025年第2期275-283,共9页
Bacterial infections of avian embryos can lead to an increase in embryo mortality,and the proliferation of antimicrobial-resistant bacteria aggravates the situation.A low hatching rate also poses a challenge to the po... Bacterial infections of avian embryos can lead to an increase in embryo mortality,and the proliferation of antimicrobial-resistant bacteria aggravates the situation.A low hatching rate also poses a challenge to the population of artificially bred Crested Ibises(Nipponia nippon).This study aims to determine the potential association between bacterial infection and the death of Crested Ibis embryos,and whether there is convergence between antimicrobial resistance and virulence in strain.In this study,13 Escherichia coli and 12 Proteus mirabilis isolates were recovered from dead Crested Ibis embryos.The pathogenicity examination confirmed the pathogenicity of all isolates,and multiple virulence genes detected by PCR-sequencing demonstrated the presence of irp2 and iuc D(100%),fim C and iss(92.31%)in E.coli,and uca A(58.33%)in P.mirabilis.Antimicrobial susceptibility test demonstrated that isolates were mainly resistant to amoxicillin(E.coli:76.92%,P.mirabilis:91.67%),cefazolin(E.coli:76.92%,P.mirabilis:91.67%),oxytetracycline(E.coli:92.31%,P.mirabilis:75.00%)and sulfamethoxazole-trimethoprim(E.coli:53.85%,P.mirabilis:33.33%),and more than 30%of isolates showed multidrug-resistance(MDR).Further analyses detected extended-spectrumβ-lactamase(ESBL)genes,of which blaTEM-1(E.coli:100%,P.mirabilis:100%)had the highest frequency,followed by the blaCTX-M-55(E.coli:92.31%,P.mirabilis:50%),blaCTX-M-14(E.coli:76.92%,P.mirabilis:33.33%),blaCTX-M-65(E.coli:15.38%,P.mirabilis:16.67%),and all isolates were negative for blaSHV and blaOXA.Pearson's correlation analysis showed a positive correlation between the presence ofβ-lactam resistance and ESBL genes,while mainly negative correlations were observed between the presence of ESBL genes and virulence genes.Furthermore,the conjugation experiment and PFGE revealed that the isolates were primarily polyclonal,and there was horizontal transfer of resistance or virulence genes by plasmids.Based on the results,E.coli and P.mirabilis were responsible for embryonic mortality of the ibises in this study.The co-presence and co-transfer of ESBL genes and virulence genes can pose a potential threat to the health of the Crested Ibis,and measures such as prudent use of antimicrobials,and constant surveillance of resistance and pathogenicity,must be implemented at the Crested Ibis breeding base. 展开更多
关键词 Dead embryo Escherichia coli Nipponia nippon Proteus mirabilis Resistance ESBL gene Virulence gene
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Genome-scale metabolic network model-guided genetic modification of Escherichia coli for pyruvate accumulation 认领 引用
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作者 LI Xuefei GUO Chaohao +4 位作者 TONG Wenyue YANG Sen LIU Xiaoyun LI Jingchen KANG Ming 《微生物学报》 CAS CSCD 北大核心 2025年第10期4374-4391,共18页
[Objective]To construct an Escherichia coli mutant strain that accumulates pyruvate by genetic modification guided by the genome-scale metabolic network model.[Methods]Using a genome-scale metabolic network model as a... [Objective]To construct an Escherichia coli mutant strain that accumulates pyruvate by genetic modification guided by the genome-scale metabolic network model.[Methods]Using a genome-scale metabolic network model as a guide,we simulated pyruvate production of E.coli,screened key genes in metabolic pathways,and developed gene editing procedures accordingly.We knocked out the acetate kinase gene ackA,phosphate acetyltransferase gene pta,alcohol dehydrogenase adhE,glycogen synthase gene glgA,glycogen phosphorylase gene glgP,phosphoribosyl pyrophosphate(PRPP)synthase gene prs,ribose 1,5-bisphosphate phosphokinase gene phnN,and transporter encoding gene proP.Furthermore,we knocked in the transporter encoding gene ompC,flavonoid toxin gene fldA,and D-serine ammonia lyase gene dsdA.[Results]A shake flask process with the genetically edited mutant strain MG1655-6-2 under anaerobic conditions produced pyruvate at a titer of 10.46 g/L and a yield of 0.69 g/g.Metabolomic analysis revealed a significant increase in the pyruvate level in the fermentation broth,accompanied by notable decreases in the levels of certain related metabolic byproducts.Through 5 L fed-batch fermentation and an adaptive laboratory evolution,the strain finally achieved a pyruvate titer of 45.86 g/L.[Conclusion]This study illustrated the efficacy of a gene editing strategy predicted by a genome-scale metabolic network model in enhancing pyruvate accumulation in E.coli under anaerobic conditions and provided novel insights for microbial metabolic engineering. 展开更多
关键词 Escherichia coli pyruvate genome-scale metabolic network model CRISPR-Cas9 adaptive laboratory evolution
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Effects of monoglyceride blend on systemic and intestinal immune responses,and gut health of weaned pigs experimentally infected with a pathogenic Escherichia coli 认领 引用 被引量：3
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作者 Sangwoo Park Shuhan Sun +5 位作者 Lauren Kovanda Adebayo O.Sokale Adriana Barri Kwangwook Kim Xunde Li Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期345-363,共19页
Background Monoglycerides have emerged as a promising alternative to conventional practices due to their biolog-ical activities,including antimicrobial properties.However,few studies have assessed the efficacy of mono... Background Monoglycerides have emerged as a promising alternative to conventional practices due to their biolog-ical activities,including antimicrobial properties.However,few studies have assessed the efficacy of monoglyceride blend on weaned pigs and their impacts on performance,immune response,and gut health using a disease chal-lenge model.Therefore,this study aimed to investigate the effects of dietary monoglycerides of short-and medium-chain fatty acids on the immunity and gut health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli F18.Results Pigs supplemented with high-dose zinc oxide(ZNO)had greater(P<0.05)growth performance than other treatments,but no difference was observed in average daily feed intake between ZNO and monoglycerides groups during the post-challenge period.Pigs in ZNO and antibiotic groups had lower(P<0.05)severity of diarrhea than control,but the severity of diarrhea was not different between antibiotic and monoglycerides groups.Pigs fed with monoglycerides or ZNO had lower(P<0.05)serum haptoglobin on d 2 or 5 post-inoculation than control.Pigs in ZNO had greater(P<0.05)goblet cell numbers per villus,villus area and height,and villus height:crypt depth ratio(VH:CD)in duodenum on d 5 post-inoculation than pigs in other treatments.Pigs supplemented with monoglyc-erides,ZNO,or antibiotics had reduced(P<0.05)ileal crypt depth compared with control on d 5 post-inoculation,contributing to the increase(P=0.06)in VH:CD.Consistently,pigs in ZNO expressed the lowest(P<0.05)TNFa,IL6,IL10,IL12,IL1A,IL1B,and PTGS2 in ileal mucosa on d 5 post-inoculation,and no difference was observed in the expres-sion of those genes between ZNO and monoglycerides.Supplementation of ZNO and antibiotic had significant impacts on metabolic pathways in the serum compared with control,particularly on carbohydrate and amino acid metabolism,while limited impacts on serum metabolites were observed in monoglycerides group when compared with control.Conclusions The results suggest that supplementation of monoglyceride blend may enhance disease resist-ance of weaned pigs by alleviating the severity of diarrhea and mitigating intestinal and systemic inflammation,although the effectiveness may not be comparable to high-dose zinc oxide. 展开更多
关键词 Diarrhea Enterotoxigenic Escherichia coli Gut health Monoglycerides Systemic immunity Weaned pigs
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Dietary supplementation of blend of organic acids and monoglycerides alleviated diarrhea and systemic inflammation of weaned pigs experimentally infected with enterotoxigenic Escherichia coli F18 认领 引用 被引量：2
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作者 Sangwoo Park Shuhan Sun +3 位作者 Supatirada Wongchanla Ying Chen Xunde Li Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第3期1255-1268,共14页
Background The emergence of antibiotic resistant microorganisms associated with conventional swine production practices has increased interest in acid-based compounds having antimicrobial properties and other biologic... Background The emergence of antibiotic resistant microorganisms associated with conventional swine production practices has increased interest in acid-based compounds having antimicrobial properties and other biological functions as nutritional interventions.Despite the interest in organic acids and monoglycerides,few studies have examined the effects of the combination of these acid-based additives in weaned pigs under disease challenge conditions.Therefore,this study aimed to investigate the effects of dietary supplementation with blend of organic acids and/or medium-chain fatty acid monoglycerides on intestinal health and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli(ETEC)F18 at 4-week of age.Results Dietary supplementation of organic acids,monoglycerides,or both organic acids and monoglycerides(combination)reduced(P<0.05)the diarrhea frequency of ETEC F18-infected pigs throughout the experimental period(d−7 to 21 post-inoculation).This is consistent with the reduced(P<0.05)proportion ofβ-hemolytic coliforms in feces observed for the organic acid and combination treatments on d 10 post-inoculation.Supplementation of organic acids,monoglycerides,or combination also reduced(P<0.05)bacterial translocation in mesenteric lymph nodes on d 21 post-inoculation.Pigs fed with monoglycerides or combination had lower(P<0.05)white blood cells on d 5 post-inoculation,and pigs fed the combination also had lower(P<0.05)lymphocytes than pigs in control group.Monoglyceride supplementation increased(P<0.05)white blood cells and neutrophils compared with control group on d 14 post-inoculation.However,supplementation with organic acid blend,monoglyceride blend,or combination did not affect growth performance in this experiment.Conclusions Supplementation with monoglycerides or organic acids alone or in combination improves the detrimental effects of ETEC F18 infection in weaned pigs,as indicated by reduced diarrhea,fecal shedding ofβ-hemolytic coliforms,and bacterial translocation,and thus enhancing disease resistance.Monoglycerides reduced the inflammatory response during peak infection,but their immunomodulatory and possible synergistic effects with organic acids need to be further investigated. 展开更多
关键词 Acidifiers Antimicrobial agents Diarrhea Enterotoxigenic Escherichia coli Monoglycerides Systemic immunity Weaned pigs
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Effects of dietary L-glutamate and L-aspartate supplementation on growth performance,severity of diarrhea,intestinal barrier integrity,and fecal microbiota of weaned piglets challenged with F18 enterotoxigenic Escherichia coli 认领 引用 被引量：1
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作者 Supatirada Wongchanla Kunal Dixit +9 位作者 Sangwoo Park Kwangwook Kim Shuhan Sun Maria Marco Steven B.Palomares Alejandra Mejia-Caballero Sahana Mohan Xunde Li Xiaojing Li Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第6期2895-2909,共15页
Background L-Glutamate and L-aspartate are functional amino acids that play pivotal roles in the cellular metabolic pathways of swine enterocytes.Therefore,this study aimed to investigate the effects of dietary L-glut... Background L-Glutamate and L-aspartate are functional amino acids that play pivotal roles in the cellular metabolic pathways of swine enterocytes.Therefore,this study aimed to investigate the effects of dietary L-glutamate and L-aspartate on growth performance,diarrhea severity,intestinal barrier integrity,and fecal microbiota of weaned piglets challenged with F18 enterotoxigenic Escherichia coli(ETEC).Weaned piglets were randomly assigned to seven dietary treatments,including unchallenged and ETEC-challenged controls,amino acid-supplemented groups,and an antibiotic control,to assess their responses to ETEC challenge.Results Supplementation with 1%L-glutamate or 2%L-aspartate enhanced growth performance,with significantly greater(P<0.05)average daily weight gain and gain-to-feed ratio compared with the positive control group from d 0 to d 5 post-inoculation.Pigs fed with 1%or 2%L-aspartate had reduced(P<0.05)diarrhea severity in ETEC-challenged pigs compared with the positive control group.The 1%L-aspartate supplementation also supported intestinal structure by increasing(P<0.05)duodenal villi height and ileal villi width compared with carbadox supplementation.Additionally,1%L-glutamate supplementation significantly improved(P<0.05)resilience in ETEC-challenged pigs by reducing fecal shedding ofβ-hemolysin-producing bacteria compared with the positive control group on d 14 post-inoculation.Moreover,1%L-aspartate supplementation promoted intestinal barrier integrity by significantly up-regulated(P<0.05)the expression of ileal OCDN and ileal ZO-1 compared with the positive control group on d 14 post-inoculation.Interestingly,2%L-aspartate supplementation altered the intestinal mucosa by down-regulating(P<0.05)the expression of jejunal CLDN-1,while up-regulating(P<0.05)the expression of ileal CLDN-1 compared with the negative control group on d 14 post-inoculation.Furthermore,L-glutamate supplementation significantly changed proportions of Firmicutes and Bacteroidota and showed the trend for enrichment in beneficial bacterial genera such as Bifidobacterium and Megasphaera in ETEC-infected pigs by d 14 post-inoculation.Conclusion Supplementation with L-glutamate or L-aspartate promoted growth performance,supported gut health,and enhanced disease resistance in weaned pigs challenged with F18 ETEC.During the weaning period,L-glutamate or L-aspartate could potentially be considered conditionally essential amino acids,helping to alleviate weaning complications and reduce the need for antibiotic use in swine farming. 展开更多
关键词 Diarrhea Escherichia coli Fecal microbiota L-Aspartate L-Glutamate Weaned pigs
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Recombinant Escherichia coli-driven whole-cell bioconversion for selective 5-Aminopentanol production as a novel bioplastic monomer 认领 引用
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作者 Byung Wook Lee Hee Taek Kim +8 位作者 Hyun Gi Koh Kyungjae Yu Gaeul Kim Yoon Jung Jung Haeng-Geun Cha Yunhee Jeong Yung-Hun Yang See-Hyoung Park Kyungmoon Park 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期944-954,共11页
5-Aminopentanol(5-AP)is a valuable amino alcohol with potential applications in polymer synthesis and bioplastics.Conventional production methods rely on petroleum-based feedstocks and metal catalysts,which raise envi... 5-Aminopentanol(5-AP)is a valuable amino alcohol with potential applications in polymer synthesis and bioplastics.Conventional production methods rely on petroleum-based feedstocks and metal catalysts,which raise environmental and sustainability concerns.In this study,a de novo biosynthetic pathway for 5-AP production from l-lysine was developed in Escherichia coli.The engineered pathway consisted of lysine decarboxylase 2(LdcC),putrescine aminotransferase(PatA),and tested aldehyde reductase(YahK,YihU,YqhD).Among the tested reductases,aldehyde reductase exhibited the highest catalytic efficiency,producing 44.5±2.6 mM of 5-AP(0.44±0.03 mol5−AP/molL−lysine).The replacement of the expression system with a T7-based dual-plasmid platform,pET24ma::ldcC,and pCDFDuet-1::yqhD::patA co-transformed into E.coli,increased the production to 60.7±5.8 mM,accompanied by reduced cadaverine accumulation.Further enhancement was achieved by increasing the gene dosage of PatA,leading to 68.5±4.2 mM 5-AP and reduced by 40%in cadaverine levels.Cadaverine is a precursor in the production of 5-AP,and its accumulation is an important factor in the limitation of conversion to 5-AP.Intracellular cofactor regeneration is expected to cause an indirect supply ofα-KG,a cofactor,to enhance conversion to 5-AP.To support intracellular cofactor regeneration,glucose supplementation and increased aeration were applied,resulting in a final titer of 78.5±1.2 mM 5-AP and improved precursor utilization.This study is the first report of selective microbial 5-AP production and highlights the importance of PatA expression in pathway optimization.The newly established l-lysine(C6)valorization process which converts l-lysine to high-value materials such as 1,5-PDO,glutarate,and 5-AP offers a promising route for the sustainable biosynthesis of amino alcohols,laying the groundwork for future improvements through enzyme engineering and metabolic design. 展开更多
关键词 5-Aminopentanol L-Lysine(C6)valorization process Whole-cell bioconversion Escherichia coli Novel bioplastic monomer
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Role and mechanisms of action of escherichia coli nissle 1917 in the maintenance of remission in ulcerative colitis patients: an update 认领 引用 被引量：29
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作者 Franco Scaldaferri Viviana Gerardi +8 位作者 Francesca Mangiola Loris Riccardo Lopetuso Marco Pizzoferrato Valentina Petito Alfredo Papa Jovana Stojanovic Andrea Poscia Giovanni Cammarota Antonio Gasbarrini 《World Journal of Gastroenterology》 SCIE CAS 2016年第24期5505-5511,共7页
Ulcerative colitis (UC) is a chronic inflammatory disease, whose etiology is still unclear. Its pathogenesis involves an interaction between genetic factors, immune response and the &#x0201c;forgotten organ&#x... Ulcerative colitis (UC) is a chronic inflammatory disease, whose etiology is still unclear. Its pathogenesis involves an interaction between genetic factors, immune response and the &#x0201c;forgotten organ&#x0201d;, Gut Microbiota. Several studies have been conducted to assess the role of antibiotics and probiotics as additional or alternative therapies for Ulcerative Colitis. Escherichia coli Nissle (EcN) is a nonpathogenic Gram-negative strain isolated in 1917 by Alfred Nissle and it is the active component of microbial drug Mutaflor&#x000ae; (Ardeypharm GmbH, Herdecke, Germany and EcN, Cadigroup, In Italy) used in many gastrointestinal disorder including diarrhea, uncomplicated diverticular disease and UC. It is the only probiotic recommended in ECCO guidelines as effective alternative to mesalazine in maintenance of remission in UC patients. In this review we propose an update on the role of EcN 1917 in maintenance of remission in UC patients, including data about efficacy and safety. Further studies may be helpful for this subject to further the full use of potential of EcN. 展开更多
关键词 Ulcerative colitis Escherichia coli Nissle Metanalysis Probiotic Randomized trial Inflammatory bowel disease
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