The multi-pass intermittent local loading process,which features a more flexible processing path,can further enhance the second material distribution during local loading,improve the formability of components,and redu...The multi-pass intermittent local loading process,which features a more flexible processing path,can further enhance the second material distribution during local loading,improve the formability of components,and reduce forming loads.However,the absence of compatible forming equipment makes it difficult to control the constraint in the unloaded zones during the forming process.This difficulty complicates coordination and control of deformation,particularly for asymmetric rib-web components.Additionally,the current implementation involves multi-fire heating,a long process flow,and high energy consumption,which limits the popularization and application of the local loading process.In this study,a new multi-pass local loading hydraulic forming apparatus that can quickly and reliably switch between heavy-load deformation and low-load constraint for different local loading sub-dies was developed.A 10-tonne laboratory prototype was developed,and the forming characteristics during the forming process as well as the response characteristics of the hydraulic system during the multi-pass intermittent local loading of rib-web component were investigated using numerical simulations and physical experiments.Results indicated that,compared to a whole loading process with the same initial geometry of billet,the total forming load(i.e.,the sum of loaded and restrained loads)is reduced by more than 40%with the local loading process,and by nearly 50%with multi-pass local loading.The multi-pass local loading process allows for more effective control of material flow compared to single-pass local loading,leading to improved cavity filling and reduced flow line disturbance.For a large-scale,complex titanium alloy bulkhead,the cavity filling problem was addressed by optimizing the multi-pass local loading path with an unequal thickness billet.The dynamic performance of the multi-pass local loading hydraulic system was found to be robust,with stable pressure transitions during motion and load switching for the sub-die(s).The dynamic characteristic of the hydraulic cylinder when switching from non-moving/unloaded state to a moving/loading state are consistent whether a load is present or not.However,the dynamic characteristics differ when switching from a moving/loading state to non-moving/unloaded state,showing opposite behavior.The developed hydraulic drive mechanism provides a way for implementation of multi-pass local loading without auxiliary operation and extra heating.The results of the study provide a foundation for the industrial production of large-scale,complex components with reduced force requirement and low-energy consumption.展开更多
Microbially-driven alkaline neutralization during soil formation in bauxite residue is a promising technology toward sustainable management of alkaline solid-waste landscapes worldwide.However,our knowledge regarding ...Microbially-driven alkaline neutralization during soil formation in bauxite residue is a promising technology toward sustainable management of alkaline solid-waste landscapes worldwide.However,our knowledge regarding the impacts of microorganisms on the organic components dynamics involved in alkalinity regulation remains rather limited.In this study,we performed water-phase and solid-phase microcosm experiments using Penicillium oxalicum and sugarcane bagasse with gradient addition amount.Multi-spectroscopic techniques and metabolomic analysis were employed to investigate the combined effects of alkaline stabilization and carbon components dynamics in bauxite residue.An addition of 5‰of sugarcane bagasse was appropriate to reduce the pH of bauxite residue from 10.16 to30%.Spectroscopic analysis verified that sugarcane bagasse predominantly formed connections with humic substances,establishing zones rich in carbon containing fulvic and humic acids,which promoted the bio-production of lowmolecular-weight-organic acids.Metabolomic analysis identified 631 compounds,with 126 associated to carbon metabolism.Notably,tricarboxylic acid cycle(TCA)pathway involving acetate and oxalate were significantly upregulated following the combined application.Mantel-test analysis further showed positive correlations between 5-hydroxyindoleacetic acid and 5-phosphonooxy-L-lysine and alkalinity regulation,proving that the combined application regulated alkalinity by improving amino acid metabolism and carbon skeleton reutilization.Overall,our findings highlighted the metabolic mechanisms involved in alkalinity regulation during soil formation process mediated by P.oxalicum and provided an optimized solution for microbially-driven alkaline regulation within bauxite residue.展开更多
To elucidate the origin of mechanical anisotropy in cylindrical Mg alloy components containing long-periodic stacking-ordered(LPSO)phases and to provide guidance for component-level microstructure design,this study sy...To elucidate the origin of mechanical anisotropy in cylindrical Mg alloy components containing long-periodic stacking-ordered(LPSO)phases and to provide guidance for component-level microstructure design,this study systematically investigated the combined effects of texture and oriented LPSO phases on the three-dimensional mechanical anisotropy of a cylindrical Mg-Gd-Y-Zn-Zr alloy component fabricated by back-extrusion.The results of uniaxial tensile tests show that there are significant differences in three-dimensional mechanical properties of cylindrical component,and the plastic anisotropy is more significant compared to the strength anisotropy.By comparing the Schmid factor(SF)distribution and slip mode of primary-texture and secondary-texture,it is revealed that primary-texture grains have a greater influence on strength anisotropy than secondary-texture grains.The larger SF difference between primary-texture grains and secondary-texture grains leads to significant gradient slip,uncoordinated strain and dislocation accumulation,which results in stronger dislocation strengthening,strain strengthening,and strength anisotropy.The fracture of blocky LPSO phases leads to straight cleavage planes,and the matrix is prone to the formation of numerous tearing ridges.The oriented blocky LPSO phase affects the strength anisotropy through load-bearing strengthening effect,and affects the plastic anisotropy by changing microcrack propagation mode and fracture mechanism.Overall,the oriented blocky LPSO phase has a more significant effect on mechanical anisotropy than the texture.展开更多
Due to high-intensity human activities,the ecological environment of the gulf has suffered severe damage that leads to ecosystem imbalances that not only threaten the survival of marine organisms but also pose remarka...Due to high-intensity human activities,the ecological environment of the gulf has suffered severe damage that leads to ecosystem imbalances that not only threaten the survival of marine organisms but also pose remarkable risks to economic and social development in coastal zones.Consequently,to understanding long-term variations in gulf water quality is an urgent need.This paper focuses on Qinzhou Bay(QZB)as the study area and analyzes the long-term sequences(2011-2023)of critical seawater parameters,including pH,salinity(Sal),dissolved oxygen(DO),chemical oxygen demand(COD),dissolved inorganic nitrogen(DIN),total dissolved nitrogen(TDN),dissolved inorganic phosphorus(DIP),total dissolved phosphorus(TDP),and chlorophyll-a(Chl-a)through comprehensive evaluations of water quality pollution status,severity,and trends using single-factor pollution indices,eutrophication indices,and principal component analysis(PCA).The findings reveal that the water quality in QZB exhibited temporal fluctuations between 2014 and 2023 and a spatially decreasing gradient from the inner bay to the outer bay regions.The comprehensive fuzzy method evaluates the QZB water quality grade as class III.The main driving factors are nitrogen and phosphorus discharged from terrestrial sources.Nitrogen and phosphorus in QZB was increased by ca.27%and ca.13%from 2011 to 2023,respectively.This paper aims to provide scientific evidence for the rational exploitation of marine resources.The findings further support the protection of marine environments,promotion of sustainable marine resource utilization,and maintenance of marine ecological balance.展开更多
Hospital-influenced bioaerosols pose critical health risks due to their pathogenic potential,yet their dynamic assembly mechanisms during vulnerable time period remain poorly characterized.Through parallel sampling of...Hospital-influenced bioaerosols pose critical health risks due to their pathogenic potential,yet their dynamic assembly mechanisms during vulnerable time period remain poorly characterized.Through parallel sampling of hospital-and school-influenced bioaerosol during summer periods,we revealed distinct diurnal fluctuations and site-specific microbial assembly patterns.HYSPLIT trajectory modeling demonstrated that school-influenced bioaerosols exhibited greater meteorological source diversity than hospital counterparts,promoting stochastic assembly processes.Conversely,co-occurrence network analysis identified deterministic assembly driven by hospital bioaerosols capacity to utilize particulate-bound polycyclic aromatic hydrocarbons(PAHs).Notably,Proteobacteria-associated pathogens exhibited environmental resilience and were significantly more abundant in hospital aerosols compared to school environments.These findings challenge the conventional view of bioaerosols as passively dispersed entities,establishing a mechanistic link between anthropogenic pollution sources and pathogenic bioaerosol persistence in summer ecosystems.Our work provides critical insights for predicting microbial risks in healthcare-associated atmospheric environments.展开更多
Polyurethane polymer grouting materials with rapid expansion and solidification characteristics have been widely applied for infrastructure repair and reinforcement.An accurate characterization of the chemical reactio...Polyurethane polymer grouting materials with rapid expansion and solidification characteristics have been widely applied for infrastructure repair and reinforcement.An accurate characterization of the chemical reaction process of the slurry is essential for investigating its grouting mechanism.However,the high concentration of isocyanate groups in such polymer systems causes a“flat-top phenomenon”in Fourier transform infrared(FTIR)spectroscopy,rendering it difficult to accurately determine the conversion rate.To address this issue,a real-time method for measuring the reaction conversion rates of slurry components was proposed.The polyol conversion rate was obtained by tracking the integral area changes of the C―O bond peak in the carbamate product relative to the internal standard.The total slurry volume was determined at different time intervals using light detection and ranging(LiDAR)-based point cloud scanning,from which the gas volume was estimated.Combined with the solubility curve of the physical blowing agent and ideal gas law,the conversion rate of the chemical blowing agent was calculated.The isocyanate conversion rate was indirectly inferred based on the measured polyol and chemical blowing agent conversions.This method was applied to a polyurethane grouting material used in an engineering project,and the time-resolved conversion curves of all components throughout the reaction were obtained.The results revealed a three-stage evolution:a slow initial increase,a rapid rise in the middle stage,and a gradual deceleration in the later stage.The foaming reaction proceeded consistently faster than the gelation reaction did.These findings provide a foundation for further research on the diffusion mechanisms of polyurethane polymer slurries.展开更多
Diesel accounts for over 60%of the products derived from direct coal liquefaction(DCL).Compared to petroleum-based diesel,DCL diesel exhibits a cetane number ranging from 30 to 40,which fails to meet the automotive di...Diesel accounts for over 60%of the products derived from direct coal liquefaction(DCL).Compared to petroleum-based diesel,DCL diesel exhibits a cetane number ranging from 30 to 40,which fails to meet the automotive diesel standard requirement of≥45.Therefore,rapid and accurate analysis of its chemical composition is crucial for property optimization to meet fuel specifications by component blending.Thought traditional methods like gas chromatography offer high accuracy,they are unsuitable for rapid online analysis under industrial conditions.Near-infrared(NIR)spectroscopy can provide advantages in rapid,non-destructive analysis.Its application however,is limited by the complexity of spectral data interpretation.Machine learning(ML)is effective method for extracting valuable information from spectra and establishing high-precision prediction models.This study integrates NIR spectroscopy with ML to construct a spectral-composition database for DCL diesel.Feature extraction was performed using the correlation coefficient and mutual information methods to screen key wavelength variables and reduce data dimensionality.Subsequently,the predictive performance of three ML models—Lasso,SVR and XGBoost—was compared.Results indicate that excluding spectral data with absorbance greater than 1 significantly enhances model accuracy,increasing the test set R2 from 0.85 to 0.96.After feature extraction,the optimal number of wavelength variables was reduced to 177,substantially improving computational efficiency.Among the models evaluated,the SVR-MI-0.9 model,based on mutual information feature selection,demonstrated the best performance,achieving training and test set R2 values both exceeding 0.98.This model enables precise prediction of paraffin,naphthene,and aromatic hydrocarbon contents.This research provides a robust methodology for intelligent online quality monitoring.An intelligent NIR spectroscopy data analysis software was independently developed based on the established model.Compared with comprehensive two-dimensional gas chromatography,the software reduced the analysis time by over 98%,with an absolute prediction error below 0.2%.Thus,rapid analysis of DCL diesel components was successfully realized.展开更多
Methylsiloxanes(MSs)are anthropogenic substances that do not occur naturally,and most studies have focused on their presence in indoor environments.To date,there is limited information regarding their prevalence in ro...Methylsiloxanes(MSs)are anthropogenic substances that do not occur naturally,and most studies have focused on their presence in indoor environments.To date,there is limited information regarding their prevalence in road dust across broad geographical scales,with even fewer studies identifying the sources of MSs.This research investigates the levels,regional distributions,and potential origins of MSs in road dust collected from major cities throughout China.The results indicate that these chemicals are prevalent in road dust,with total concentrations of the target MSs ranging from 6.36 to 8618 ng/g dw.Tetradecamethylcycloheptasiloxane was identified as the dominant methylsiloxane.Spatial variations were primarily associated with traffic flow,the value added by the secondary industry,and local population density.Principal component analysis combined with multiple linear regression and positive matrix factorization revealed that vehicle emissions(41.8%–42.3%),industrial activities(38.9%–47.2%),and the use of personal care and consumer products(11.0%–18.8%)were the major contributors to MSs in road dust.The overall source contributions identified by both methods were consistent.These findings highlight the significant role of vehicle emissions and industrial activities and provide valuable insights for developing policies aimed at managing MSs in urban areas.展开更多
Grain-size sensitive component separation(GSCS)methods are pivotal for paleoenvironmental reconstruction but remain underexplored in tectonically active deep-sea seamount settings like the central South China Sea(SCS)...Grain-size sensitive component separation(GSCS)methods are pivotal for paleoenvironmental reconstruction but remain underexplored in tectonically active deep-sea seamount settings like the central South China Sea(SCS).This study presents the first systematic comparison of three GSCS techniques,namely standard deviation(STD),endmember modeling(EMM),and principal component analysis(PCA).These methods are applied specifically to sediment core GT-06 recovered from the Zhongnan Seamount in the central South China Sea.By integrating grain-size unmixing with complementary proxies,including magnetic susceptibility(MS)and loss on ignition(LOI),we assess the resolving power of each method and relate the resulting components to site-specific sedimentary processes(e.g.,summer monsoon,volcanic activity,seamount collapse-induced turbidity currents).Key results show that all methods identified three dominant grain-size ranges(clay,silt-sand,coarse sand)with robust inter-method correlations.Critically,EMM and PCA uniquely resolved a volcanic-derived silt component(EM2)that was undetectable using the STD method,highlighting a key limitation of STD in complex settings.Four geologically meaningful end-members were identified:(1)fine-grained terrigenous clay linked to the East Asian summer monsoon(EASM),(2)volcanic detritus reflecting Quaternary submarine eruptions,(3)siliceous biogenic debris indicative of monsoon-modulated productivity,and(4)coarse calcareous fragments associated with seamount collapse-induced turbidity currents.The results highlight the superior ability of EMM/PCA to resolve complex signals,whereas STD serves as an efficient yet limited tool for first-order screening.Together,these methods form a site-adapted framework for tectonically active deep-sea seamounts:EMM/PCA enabling fine-scale interpretation of monsoon-volcanic-turbidity interactions,and STD supports rapid large-dataset comparison.This workflow improves the reliability of paleoenvironmental reconstructions in mixed-signal settings like the Zhongnan Seamount.展开更多
Considering the complex occurrence environment and significant compositional variation of continental shale oil,as well as the uncertainties in its mobility and producible amount,this study employs geochemical analysi...Considering the complex occurrence environment and significant compositional variation of continental shale oil,as well as the uncertainties in its mobility and producible amount,this study employs geochemical analysis and production monitoring to investigate the"component flow"phenomenon of shale oil during production from the Permian Lucaogou Formation in the Jimsar Sag,Junggar Basin.It is clarified that the miscibility of different hydrocarbon components and non-hydrocarbon substances improves the flowability of multi-component hydrocarbons and non-hydrocarbons,thereby effectively enhancing the production of shale oil.Research indicates that the"lower sweet spot"has a relatively high content of light and medium hydrocarbon components and strong formation energy compared to the"upper sweet spot"of Lucaogou Formation,resulting in higher density and viscosity of the produced crude oil,which can be regarded as evidence of"component flow"of retained hydrocarbons.The"upper sweet spot"exhibits two scenarios.In areas far from faults with good preservation conditions,the high content of light and medium components in retained hydrocarbons and a high formation pressure coefficient make component flow more likely to occur.Consequently,the produced crude oil has a higher density,and the estimated ultimate recovery(EUR)per well is also higher.In areas near faults with poor preservation conditions,although the produced crude oil has a light density,the EUR per well is relatively low,indicating that the conditions for component flow of retained hydrocarbons underground have deteriorated.The study also demonstrates that preservation conditions(preventing light hydrocarbon escape and maintaining formation energy)and production strategies(controlling production pressure differential and maintaining stable operations)are important factors in regulating the occurrence and continuity of"component flow"to maximize EUR per well.These new insights can be applied to the evaluation of economically productive"sweet spots"and provide guidance for achieving optimal EUR per well in shale oil production.展开更多
Crassostrea gigas has good taste and high nutritional value;however,there are few assessments of comprehensive and panoramic analyses of the nutritional quality of the northern oyster.To study the nutritional characte...Crassostrea gigas has good taste and high nutritional value;however,there are few assessments of comprehensive and panoramic analyses of the nutritional quality of the northern oyster.To study the nutritional characteristics of C.gigas from different sources(ploidy,region,size,and culture mode),C.gigas from various ploidy(diploid and triploid),regions(Rushan,Off-site fattening,and Rongcheng),sizes(small,medium,and large)and culture modes(nearshore and offshore)were selected for comparative analyses.The nutritional components(moisture,protein,fat,and mineral),flavor substances(taste amino acids,nucleotides,and succinic acid),and functional indices(eicosapentaenoic acid(EPA),docosahexaenoic acid(DHA),and taurine)of C.gigas were determined.Principal component analysis(PCA)was used to comprehensively evaluate the oysters and investigate the variations in nutritional quality.The PCA results indicate that protein,essential fatty acids,selenium,zinc,taste amino acids,taurine,EPA,and DHA were core components contributing to 82.25%of the cumulative variance,providing a more comprehensive reflection of the nutrient composition of C.gigas.The extensive quality rankings for the C.gigas were as follows:diploid>triploid,Rushan>fattening>Rongcheng,medium>large>small,and offshore>nearshore.The score rank revealed that diploid oysters of medium-size from Rushan demonstrated superior nutritional quality compared to other tested samples.This is the first comprehensive and systematic investigation of C.gigas in northern China to reveal the feature of nutrients,flavor,and functional components.The study provided data support for the culture,consumption,processing,research,and nutritional quality improvement of oyster industry.展开更多
Understanding the synergistic mechanism between root mechanical effects and soil ecological effects is essential for revealing the dynamic evolution of root reinforcement.However,how this synergy evolves with plant gr...Understanding the synergistic mechanism between root mechanical effects and soil ecological effects is essential for revealing the dynamic evolution of root reinforcement.However,how this synergy evolves with plant growth stages remains unclear.To address this,a 360-day outdoor experiment was conducted using three herbaceous species:Cynodon dactylon,Trifolium repens,and Lolium perenne.Root-soil composites,unrooted soils,and intact roots were sampled at seven intervals for the measurement of disintegration rate,shear strength,soil physicochemical properties,root morphology,and root mechanical properties.Principal component analysis(PCA)identified organic matter,tensile force,and root length density as key loading factors.The results revealed a clear stage-dependent synergy:during the early stage(30-90 days),root mechanical effects(root winding and tensile force)dominated reinforcement;during the mid-to-late stage(90-360 days),soil ecological effects(organic matter accumulation and aggregate stability)progressively strengthened and co-drove reinforcement together with root traits.This dynamic synergy explained the overall reinforcement order(L.perenne>T.repens>C.dactylon)and the distinct decline observed on day 270 due to growth cycles.Notably,L.perenne exhibited earlier establishment advantages,whereas T.repens showed greater reinforcement potential than C.dactylon during the later stages.The PCA-based Y-values(0.92-5.83)integrated both effects.It is concluded that root reinforcement evolves from a mechanically dominated phase to a mechanically-ecological synergistic phase.This stage-dependent synergy pattern provides a mechanistic basis for the dynamic evaluation of root reinforcement and for vegetation selection according to soil stabilization requirements at different growth stages.展开更多
Titanium alloy large-scale rib-web components,known for their lightweight and highstrength properties,have the potential to substantially lighten the load-bearing components of aircraft.Isothermal Local Loading(ILL)ha...Titanium alloy large-scale rib-web components,known for their lightweight and highstrength properties,have the potential to substantially lighten the load-bearing components of aircraft.Isothermal Local Loading(ILL)has been recognized as an advanced technique for the integrated and less-loading forming of these components.However,material transfer in the transitional region during ILL often leads to folding defect near the die-partition line.To this end,the position of the neutral layer during ILL is calculated via the slab method,which is modified to align with the material flow in the transitional region,taking into account the multi-stage stress states under diverse boundary conditions.By utilizing this calculation model,the material transfer rate and rib-groove filling height can be ascertained.The material transfer rate serves as a criterion for predicting folding defect,and thereby an optimal billet is obtained and implemented in the ILL process.The results demonstrate that the material transfer rate is significantly decreased compared to the non-optimized billet,resulting in a non-folding component of transitional region.展开更多
Blue light and ultraviolet-A(UV-A)radiation are critical light-quality regulators that coordinately modulate plant growth and quality in protected horticulture.However,their interactive effects on celery(Apium graveol...Blue light and ultraviolet-A(UV-A)radiation are critical light-quality regulators that coordinately modulate plant growth and quality in protected horticulture.However,their interactive effects on celery(Apium graveolens L.)remain inadequately characterized.In the present study,we quantified the individual and combined impacts of supplemental UV-A and blue light on the growth,biomass accumulation,photosynthetic performance,and nutritional quality of celery(cv.Dayehuang)in a plant factory with artificial lighting.Our results demonstrated that sole UV-A or blue light application significantly increased biomass,nutritional quality,and light use efficiency(LUE)of celery.Notably,combined supplementation with UV-A and blue light did not enhance chlorophyll content,biomass accumulation,or nutritional quality in celery relative to supplementation with either light quality alone.The principal component analysis(PCA)score plot exhibited clear separation among the four light treatments,with the UV-A treatment distinctly separated along the positive PC1 axis.Our findings demonstrate that UV-A and blue light regulate plant growth and quality via interactive rather than additive effects.Additionally,sole UV-A irradiation significantly improved celery growth and LUE.These findings provide a theoretical foundation for optimizing the light environment for celery under protected cultivation.展开更多
The physicochemical properties of SUS304 foil surfaces are crucial to their applications.Pulsed laser modification was applied to 30μm thick SUS304 foils to systematically investigate the influence of laser energy on...The physicochemical properties of SUS304 foil surfaces are crucial to their applications.Pulsed laser modification was applied to 30μm thick SUS304 foils to systematically investigate the influence of laser energy on surface characteristics.Through multidimensional characterization of surface morphology,three-dimensional profiles and roughness,contact angle,and chemical composition,the structure-function correlation between laser energy and the physicochemical properties of steel surface was revealed.With increasing laser energy,the surface morphology of the steel transitions from a directional rolling-marked structure to a uniform sponge-like isotropic structure,accompanied by increased peak density and an expanded interfacial area.Additionally,the chemical state on the metal surface gradually stabilizes from unstable redox reactions,forming a stable oxide layer and significantly increasing active hydroxyl groups,thereby effectively improving surface wettability.Single lap shear tests reveal an enhancement in the bonding strength of steel/carbon fiber reinforced composites joints after laser modification,which is attributed to the synergistic effects of mechanical interlocking,enhanced wettability,and chemical bonding at the interface.The demonstrated potential of laser surface treatment for modifying SUS304 ultra-thin foils provides theoretical support and technical reference for its application in fiber metal laminates.展开更多
Establishing a robust evaluation model for the soil erosion sensitivity index(SESI)is a fundamental prerequisite for the scientific assessment of regional soil erosion.Furthermore,understanding its spatiotemporal evol...Establishing a robust evaluation model for the soil erosion sensitivity index(SESI)is a fundamental prerequisite for the scientific assessment of regional soil erosion.Furthermore,understanding its spatiotemporal evolution and underlying driving mechanisms is essential for developing differentiated and refined regional optimization strategies.This study takes Fujian Province—a typical red soil erosion-vulnerable area in southern China—as a case study.Using spatial principal component analysis(SPCA)and geographical detectors,we selected seven factors(slope,relief amplitude,soil type,annual precipitation,normalized difference vegetation index(NDVI),population density,and land use type)to construct an evaluation indicator system for SESI.The spatiotemporal dynamics and influencing factors of SESI in Fujian Province were then analyzed.The results show that:(1)The spatial distribution pattern of SESI levels in Fujian Province remained relatively stable over the study period and exhibited significant spatial autocorrelation,characterized by high-high and low-low clustering.(2)SESI in Fujian Province generally transitioned from improving sensitivity along the southeastern coast to worsening sensitivity in the northwestern inland areas.(3)SESI was primarily controlled by natural background conditions,particularly topography and landform,with secondary influences from human activities.The driving effects of different factors were mainly manifested through interactive effects,with interaction types evolving from weak interaction to strong interaction,and finally to balanced interaction.展开更多
Rockbursts frequently occur during deep mining.They are catastrophic failures of the coal-rock composite(CRC);however,identifying their precursory signals remains challenging.This study presents a principal component ...Rockbursts frequently occur during deep mining.They are catastrophic failures of the coal-rock composite(CRC);however,identifying their precursory signals remains challenging.This study presents a principal component analysis(PCA)-density-based spatial clustering of applications with noise(DBSCAN)-assisted workflow for warning-time analysis of CRCs.Conventional uniaxial and cyclic loading-unloading tests were conducted on CRC with four different roof rock lithologies.PCA was used to reduce redundancy among multiple acoustic emission(AE)parameters,and DBSCAN was used to organize AE samples in the reduced feature space for subsequent warning-time analysis.The results indicate an inflection point in the axial strain rate before failure,which may reflect a transition in the deformation state of the composite near the critical stage.AE signal evolution shows distinct stages,with AE count and AE energy rising sharply near failure.The PCA-DBSCAN-assisted workflow identifies different warningrelated patterns:responses in the coal body are more dispersed and appear earlier,whereas responses in the roof rock are more concentrated closer to failure.The workflow provides a structured basis for organizing correlated AE responses and comparatively analyzing warning-related evolution in the coal body and the roof rock.Importantly,warning time is affected by roof lithology and generally appears earlier in the coal body than in the roof rock.These findings provide a laboratory-scale basis for comparative warning analysis of CRCs under different lithological conditions.展开更多
Pollution of potentially harmful elements(PHEs)poses a significant challenge to an urban environment.Fine roadway dust(FRD,particle size<63μm)often accumulates elevated concentrations of PHEs,posing a threat to th...Pollution of potentially harmful elements(PHEs)poses a significant challenge to an urban environment.Fine roadway dust(FRD,particle size<63μm)often accumulates elevated concentrations of PHEs,posing a threat to the ecological environment and human health,while also curbing urban sustainable development.In this study,a source–receptor model combined with one-dimensional Monte Carlo simulation(1-D MCS)and two-dimensional Monte Carlo simulation(2-D MCS)was used to assess the probabilistic eco-health risks of PHEs in FRD from Xingtai,a representative industrial city in northern China.The average concentrations of Ba,Cr,Cu,Mn,Ni,Pb,V,and Zn exceeded the background values of the local soils,and the overall degree of contamination was moderate.The PHEs originated from traffic,industrial,and natural sources,accounting for 44.2%,37.8%,and 18.0%of the total sources of PHEs,respectively.The overall eco-risk of PHEs was relatively low,whereas PHEs could pose a certain non-carcinogenic risk to children.The cancer risks to all populations,especially children,cannot be ignored.Cr was a priority pollutant,and industrial sources were the primary control targets.This study recommended the threshold values of exposure concentrations using the 2-D MCS model.The results showed that the cancer risk to children exceeded the safety threshold at Cr levels higher than 62.67 mg/kg,and Pb could pose a potential ecological risk to the environment at concentrations reaching 174.99 mg/kg.This study provides scientific guidance for pollution control and risk management of PHEs in industrial cities worldwide.展开更多
Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containin...Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containing 2(ZMIZ2)and minichromosome maintenance complex component 3(MCM3)in TNBC progression.Methods:The relationship between ZMIZ2 expression and clinical characteristics of TNBC was investigated.In vitro and in vivo experiments were performed to investigate the role of ZMIZ2 dysregulation in TNBC cell malignant behaviors.The regulatory relationship between ZMIZ2 and MCM3 was also explored.Transcriptome sequencing was performed to elucidate possible mechanisms underlying the ZMIZ2/MCM3 axis in TNBC.Results:High ZMIZ2 expression levels were associated with the malignant degree of TNBC.ZMIZ2 overexpression promoted TNBC cell proliferation,migration,and invasion;inhibited apoptosis;and induced G1 phase cell cycle arrest,whereas knockdown of ZMIZ2 had the opposite effect.ZMIZ2 directly targeted and positively regulated MCM3 expression.MCM3 knockdown reversed the effect of ZMIZ2 overexpression on TNBC tumor growth both in vitro and in vivo.High MCM3 expression levels were linked to the degree of malignancy and poor prognosis in TNBC.The differentially expressed genes associated with the ZMIZ2/MCM3 axis were significantly enriched in multiple pathways,such as the mitogen-activated protein kinase(MAPK),mechanistic target of rapamycin(mTOR),Wnt,and Ras signaling pathways,as verified by The Cancer Genome Atlas data.Conclusions:ZMIZ2 and MCM3 were highly expressed in TNBC.ZMIZ2 promoted the development by positively regulating MCM3 expression.Key pathways,such as the Ras/MAPK,phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mTOR,and Wnt signaling pathways,may be key downstreammechanisms.展开更多
Benefitting from the non-invasive and quantitative detection property,liquid NMR spectroscopy shows broad applications in the identification,separation,and structural determination of chemical substances in mixtures.H...Benefitting from the non-invasive and quantitative detection property,liquid NMR spectroscopy shows broad applications in the identification,separation,and structural determination of chemical substances in mixtures.However,its applications,especially to probing complex mixtures that contain abundant components and complicated molecular structures,somewhat encounter the challenge of spectral congestion.In this study,we demonstrate the ultra-selective extraction of targeted components from overlapped NMR spectra for mixture analyses by investigating the applications of the recent 1D selective GEMSTONE-TOCSY approach.This strategy first adopts an ultra-selective chemical shift filter to choose the targeted spins related to the components of interest,even though situated at overlapped spectral regions with decent suppression on unwanted surrounding signals.Sequentially,it utilizes the 1D selective TOCSY scheme to accurately extract total coupling networks associated with targeted spins,and then spectroscopically separate individual components,thus resolving overlapped NMR spectra.Apart from the measurements on electrolyte and sugar mixtures,experimental results demonstrate that it also allows one to analyze heterogeneous biological tissues containing plentiful metabolites and intrinsic field inhomogeneity via specific probe access to targeted components.Therefore,this study demonstrates a useful tool for component analyses and molecular structure measurements on complex chemical and biological mixtures,and it shows promising prospects for wide applications in the fields of chemistry,biology,energy,etc.展开更多
基金the supports of the National Natural Science Foundation of China(Grant No.52375378)。
摘要The multi-pass intermittent local loading process,which features a more flexible processing path,can further enhance the second material distribution during local loading,improve the formability of components,and reduce forming loads.However,the absence of compatible forming equipment makes it difficult to control the constraint in the unloaded zones during the forming process.This difficulty complicates coordination and control of deformation,particularly for asymmetric rib-web components.Additionally,the current implementation involves multi-fire heating,a long process flow,and high energy consumption,which limits the popularization and application of the local loading process.In this study,a new multi-pass local loading hydraulic forming apparatus that can quickly and reliably switch between heavy-load deformation and low-load constraint for different local loading sub-dies was developed.A 10-tonne laboratory prototype was developed,and the forming characteristics during the forming process as well as the response characteristics of the hydraulic system during the multi-pass intermittent local loading of rib-web component were investigated using numerical simulations and physical experiments.Results indicated that,compared to a whole loading process with the same initial geometry of billet,the total forming load(i.e.,the sum of loaded and restrained loads)is reduced by more than 40%with the local loading process,and by nearly 50%with multi-pass local loading.The multi-pass local loading process allows for more effective control of material flow compared to single-pass local loading,leading to improved cavity filling and reduced flow line disturbance.For a large-scale,complex titanium alloy bulkhead,the cavity filling problem was addressed by optimizing the multi-pass local loading path with an unequal thickness billet.The dynamic performance of the multi-pass local loading hydraulic system was found to be robust,with stable pressure transitions during motion and load switching for the sub-die(s).The dynamic characteristic of the hydraulic cylinder when switching from non-moving/unloaded state to a moving/loading state are consistent whether a load is present or not.However,the dynamic characteristics differ when switching from a moving/loading state to non-moving/unloaded state,showing opposite behavior.The developed hydraulic drive mechanism provides a way for implementation of multi-pass local loading without auxiliary operation and extra heating.The results of the study provide a foundation for the industrial production of large-scale,complex components with reduced force requirement and low-energy consumption.
基金supported by the Key project of the National Natural Science Foundation of China(No.42030711)the National Natural Science Foundation of China(Nos.42477437 and 42177391)the Science and Technology Innovation Program of Hunan Province(No.2024RC3041).
摘要Microbially-driven alkaline neutralization during soil formation in bauxite residue is a promising technology toward sustainable management of alkaline solid-waste landscapes worldwide.However,our knowledge regarding the impacts of microorganisms on the organic components dynamics involved in alkalinity regulation remains rather limited.In this study,we performed water-phase and solid-phase microcosm experiments using Penicillium oxalicum and sugarcane bagasse with gradient addition amount.Multi-spectroscopic techniques and metabolomic analysis were employed to investigate the combined effects of alkaline stabilization and carbon components dynamics in bauxite residue.An addition of 5‰of sugarcane bagasse was appropriate to reduce the pH of bauxite residue from 10.16 to30%.Spectroscopic analysis verified that sugarcane bagasse predominantly formed connections with humic substances,establishing zones rich in carbon containing fulvic and humic acids,which promoted the bio-production of lowmolecular-weight-organic acids.Metabolomic analysis identified 631 compounds,with 126 associated to carbon metabolism.Notably,tricarboxylic acid cycle(TCA)pathway involving acetate and oxalate were significantly upregulated following the combined application.Mantel-test analysis further showed positive correlations between 5-hydroxyindoleacetic acid and 5-phosphonooxy-L-lysine and alkalinity regulation,proving that the combined application regulated alkalinity by improving amino acid metabolism and carbon skeleton reutilization.Overall,our findings highlighted the metabolic mechanisms involved in alkalinity regulation during soil formation process mediated by P.oxalicum and provided an optimized solution for microbially-driven alkaline regulation within bauxite residue.
基金Project(2024YFB3411200)supported by the National Key Research and Development Program of ChinaProjects(U1837207,52205433)supported by the National Natural Science Foundation of China+1 种基金Project(2022JJ40608)supported by the Natural Science Foundation of Youth Science Foundation Project of Hunan Province,ChinaProject(ZZYJKT2023-04)supported by the Research Fund of State Key Laboratory of Precision Manufacturing for Extreme Service Performance,China。
摘要To elucidate the origin of mechanical anisotropy in cylindrical Mg alloy components containing long-periodic stacking-ordered(LPSO)phases and to provide guidance for component-level microstructure design,this study systematically investigated the combined effects of texture and oriented LPSO phases on the three-dimensional mechanical anisotropy of a cylindrical Mg-Gd-Y-Zn-Zr alloy component fabricated by back-extrusion.The results of uniaxial tensile tests show that there are significant differences in three-dimensional mechanical properties of cylindrical component,and the plastic anisotropy is more significant compared to the strength anisotropy.By comparing the Schmid factor(SF)distribution and slip mode of primary-texture and secondary-texture,it is revealed that primary-texture grains have a greater influence on strength anisotropy than secondary-texture grains.The larger SF difference between primary-texture grains and secondary-texture grains leads to significant gradient slip,uncoordinated strain and dislocation accumulation,which results in stronger dislocation strengthening,strain strengthening,and strength anisotropy.The fracture of blocky LPSO phases leads to straight cleavage planes,and the matrix is prone to the formation of numerous tearing ridges.The oriented blocky LPSO phase affects the strength anisotropy through load-bearing strengthening effect,and affects the plastic anisotropy by changing microcrack propagation mode and fracture mechanism.Overall,the oriented blocky LPSO phase has a more significant effect on mechanical anisotropy than the texture.
基金supported by grants from the Guangxi Natural Science Foundation(Nos.2025GXNSFHA069202 and 2025GXNSFDA02850005)the Natural Science Foundation of China(Nos.42576261,41966002,and 42166002)+4 种基金the Guangxi Natural Science Foundation(No.2021GXNSFDA075004)the Natural Science Foundation of China Shiptime Sharing Project(No.42349911)for supporting the Beibu Gulf cruise(No.NORC2024-11)the Guangxi Key Laboratory of Marine Environmental Change and Disaster in Beibu Gulf(Nos.2023ZZ01,2020KF02,and 2020ZD01)the Scientific Research Capacity Building Project for Beibu Gulf Marine Ecological Environment Field ObservationResearch Station of Guangxi(No.Guike23-026-271)。
摘要Due to high-intensity human activities,the ecological environment of the gulf has suffered severe damage that leads to ecosystem imbalances that not only threaten the survival of marine organisms but also pose remarkable risks to economic and social development in coastal zones.Consequently,to understanding long-term variations in gulf water quality is an urgent need.This paper focuses on Qinzhou Bay(QZB)as the study area and analyzes the long-term sequences(2011-2023)of critical seawater parameters,including pH,salinity(Sal),dissolved oxygen(DO),chemical oxygen demand(COD),dissolved inorganic nitrogen(DIN),total dissolved nitrogen(TDN),dissolved inorganic phosphorus(DIP),total dissolved phosphorus(TDP),and chlorophyll-a(Chl-a)through comprehensive evaluations of water quality pollution status,severity,and trends using single-factor pollution indices,eutrophication indices,and principal component analysis(PCA).The findings reveal that the water quality in QZB exhibited temporal fluctuations between 2014 and 2023 and a spatially decreasing gradient from the inner bay to the outer bay regions.The comprehensive fuzzy method evaluates the QZB water quality grade as class III.The main driving factors are nitrogen and phosphorus discharged from terrestrial sources.Nitrogen and phosphorus in QZB was increased by ca.27%and ca.13%from 2011 to 2023,respectively.This paper aims to provide scientific evidence for the rational exploitation of marine resources.The findings further support the protection of marine environments,promotion of sustainable marine resource utilization,and maintenance of marine ecological balance.
基金supported by the Fundamental Research Program of Shanxi Province(Nos.202103021224035 and 20210302123460)the Research Support Project for Returned Overseas in Shanxi Province(No.2020–011)Shanxi Laboratory for Yellow River(No.YRL-202101)。
摘要Hospital-influenced bioaerosols pose critical health risks due to their pathogenic potential,yet their dynamic assembly mechanisms during vulnerable time period remain poorly characterized.Through parallel sampling of hospital-and school-influenced bioaerosol during summer periods,we revealed distinct diurnal fluctuations and site-specific microbial assembly patterns.HYSPLIT trajectory modeling demonstrated that school-influenced bioaerosols exhibited greater meteorological source diversity than hospital counterparts,promoting stochastic assembly processes.Conversely,co-occurrence network analysis identified deterministic assembly driven by hospital bioaerosols capacity to utilize particulate-bound polycyclic aromatic hydrocarbons(PAHs).Notably,Proteobacteria-associated pathogens exhibited environmental resilience and were significantly more abundant in hospital aerosols compared to school environments.These findings challenge the conventional view of bioaerosols as passively dispersed entities,establishing a mechanistic link between anthropogenic pollution sources and pathogenic bioaerosol persistence in summer ecosystems.Our work provides critical insights for predicting microbial risks in healthcare-associated atmospheric environments.
基金supported by the National Natural Science Foundation of China(No.52178401)Science and Technology Innovation Team Support Program for Henan Universities(No.23IRTSTHN014)+2 种基金Natural Science Foundation of Henan Province of China(No.252300421251)National Natural Science Foundation of China(Nos.52578540 and 52478477)the Cross-disciplinary Innovation Research Group Project of the Natural Science Foundation of Henan Province(No.252300421827)。
摘要Polyurethane polymer grouting materials with rapid expansion and solidification characteristics have been widely applied for infrastructure repair and reinforcement.An accurate characterization of the chemical reaction process of the slurry is essential for investigating its grouting mechanism.However,the high concentration of isocyanate groups in such polymer systems causes a“flat-top phenomenon”in Fourier transform infrared(FTIR)spectroscopy,rendering it difficult to accurately determine the conversion rate.To address this issue,a real-time method for measuring the reaction conversion rates of slurry components was proposed.The polyol conversion rate was obtained by tracking the integral area changes of the C―O bond peak in the carbamate product relative to the internal standard.The total slurry volume was determined at different time intervals using light detection and ranging(LiDAR)-based point cloud scanning,from which the gas volume was estimated.Combined with the solubility curve of the physical blowing agent and ideal gas law,the conversion rate of the chemical blowing agent was calculated.The isocyanate conversion rate was indirectly inferred based on the measured polyol and chemical blowing agent conversions.This method was applied to a polyurethane grouting material used in an engineering project,and the time-resolved conversion curves of all components throughout the reaction were obtained.The results revealed a three-stage evolution:a slow initial increase,a rapid rise in the middle stage,and a gradual deceleration in the later stage.The foaming reaction proceeded consistently faster than the gelation reaction did.These findings provide a foundation for further research on the diffusion mechanisms of polyurethane polymer slurries.
基金Supported by National Natural Science Foundation of China(U24B6018,22178243)。
摘要Diesel accounts for over 60%of the products derived from direct coal liquefaction(DCL).Compared to petroleum-based diesel,DCL diesel exhibits a cetane number ranging from 30 to 40,which fails to meet the automotive diesel standard requirement of≥45.Therefore,rapid and accurate analysis of its chemical composition is crucial for property optimization to meet fuel specifications by component blending.Thought traditional methods like gas chromatography offer high accuracy,they are unsuitable for rapid online analysis under industrial conditions.Near-infrared(NIR)spectroscopy can provide advantages in rapid,non-destructive analysis.Its application however,is limited by the complexity of spectral data interpretation.Machine learning(ML)is effective method for extracting valuable information from spectra and establishing high-precision prediction models.This study integrates NIR spectroscopy with ML to construct a spectral-composition database for DCL diesel.Feature extraction was performed using the correlation coefficient and mutual information methods to screen key wavelength variables and reduce data dimensionality.Subsequently,the predictive performance of three ML models—Lasso,SVR and XGBoost—was compared.Results indicate that excluding spectral data with absorbance greater than 1 significantly enhances model accuracy,increasing the test set R2 from 0.85 to 0.96.After feature extraction,the optimal number of wavelength variables was reduced to 177,substantially improving computational efficiency.Among the models evaluated,the SVR-MI-0.9 model,based on mutual information feature selection,demonstrated the best performance,achieving training and test set R2 values both exceeding 0.98.This model enables precise prediction of paraffin,naphthene,and aromatic hydrocarbon contents.This research provides a robust methodology for intelligent online quality monitoring.An intelligent NIR spectroscopy data analysis software was independently developed based on the established model.Compared with comprehensive two-dimensional gas chromatography,the software reduced the analysis time by over 98%,with an absolute prediction error below 0.2%.Thus,rapid analysis of DCL diesel components was successfully realized.
基金supported by the National Natural Science Foundation of China(No.41771511)the Natural Science Foundation of Henan Province,China(No.252300421469)+1 种基金the Excellent Science and Technology Innovation Team of Henan Normal University(No.2021TD03)the National Scientific Research Project Cultivation Fund of Henan Normal University(No.2021PL12).
摘要Methylsiloxanes(MSs)are anthropogenic substances that do not occur naturally,and most studies have focused on their presence in indoor environments.To date,there is limited information regarding their prevalence in road dust across broad geographical scales,with even fewer studies identifying the sources of MSs.This research investigates the levels,regional distributions,and potential origins of MSs in road dust collected from major cities throughout China.The results indicate that these chemicals are prevalent in road dust,with total concentrations of the target MSs ranging from 6.36 to 8618 ng/g dw.Tetradecamethylcycloheptasiloxane was identified as the dominant methylsiloxane.Spatial variations were primarily associated with traffic flow,the value added by the secondary industry,and local population density.Principal component analysis combined with multiple linear regression and positive matrix factorization revealed that vehicle emissions(41.8%–42.3%),industrial activities(38.9%–47.2%),and the use of personal care and consumer products(11.0%–18.8%)were the major contributors to MSs in road dust.The overall source contributions identified by both methods were consistent.These findings highlight the significant role of vehicle emissions and industrial activities and provide valuable insights for developing policies aimed at managing MSs in urban areas.
基金The Guangxi Scientific Projects under contract No.2025GXNSFAA069160the Guangxi Science and Technology Program under contract No.AD25069075+2 种基金the National Natural Science Foundation of China under contract No.42366002the Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Program under contract Nos SML2021SI1004,SML2023SP215,SML2023SP218,and SML2023SP238the NSFC Shiptime Sharing Project under contract Nos 42349911,42449910,and 42449911。
摘要Grain-size sensitive component separation(GSCS)methods are pivotal for paleoenvironmental reconstruction but remain underexplored in tectonically active deep-sea seamount settings like the central South China Sea(SCS).This study presents the first systematic comparison of three GSCS techniques,namely standard deviation(STD),endmember modeling(EMM),and principal component analysis(PCA).These methods are applied specifically to sediment core GT-06 recovered from the Zhongnan Seamount in the central South China Sea.By integrating grain-size unmixing with complementary proxies,including magnetic susceptibility(MS)and loss on ignition(LOI),we assess the resolving power of each method and relate the resulting components to site-specific sedimentary processes(e.g.,summer monsoon,volcanic activity,seamount collapse-induced turbidity currents).Key results show that all methods identified three dominant grain-size ranges(clay,silt-sand,coarse sand)with robust inter-method correlations.Critically,EMM and PCA uniquely resolved a volcanic-derived silt component(EM2)that was undetectable using the STD method,highlighting a key limitation of STD in complex settings.Four geologically meaningful end-members were identified:(1)fine-grained terrigenous clay linked to the East Asian summer monsoon(EASM),(2)volcanic detritus reflecting Quaternary submarine eruptions,(3)siliceous biogenic debris indicative of monsoon-modulated productivity,and(4)coarse calcareous fragments associated with seamount collapse-induced turbidity currents.The results highlight the superior ability of EMM/PCA to resolve complex signals,whereas STD serves as an efficient yet limited tool for first-order screening.Together,these methods form a site-adapted framework for tectonically active deep-sea seamounts:EMM/PCA enabling fine-scale interpretation of monsoon-volcanic-turbidity interactions,and STD supports rapid large-dataset comparison.This workflow improves the reliability of paleoenvironmental reconstructions in mixed-signal settings like the Zhongnan Seamount.
基金Supported by the Joint Fund Project of the National Natural Science Foundation(U22B6004)National Postdoctoral Funding Program(GZC20242013)Petro China Scientific Research and Technological Development Project(2022yjcq03).
摘要Considering the complex occurrence environment and significant compositional variation of continental shale oil,as well as the uncertainties in its mobility and producible amount,this study employs geochemical analysis and production monitoring to investigate the"component flow"phenomenon of shale oil during production from the Permian Lucaogou Formation in the Jimsar Sag,Junggar Basin.It is clarified that the miscibility of different hydrocarbon components and non-hydrocarbon substances improves the flowability of multi-component hydrocarbons and non-hydrocarbons,thereby effectively enhancing the production of shale oil.Research indicates that the"lower sweet spot"has a relatively high content of light and medium hydrocarbon components and strong formation energy compared to the"upper sweet spot"of Lucaogou Formation,resulting in higher density and viscosity of the produced crude oil,which can be regarded as evidence of"component flow"of retained hydrocarbons.The"upper sweet spot"exhibits two scenarios.In areas far from faults with good preservation conditions,the high content of light and medium components in retained hydrocarbons and a high formation pressure coefficient make component flow more likely to occur.Consequently,the produced crude oil has a higher density,and the estimated ultimate recovery(EUR)per well is also higher.In areas near faults with poor preservation conditions,although the produced crude oil has a light density,the EUR per well is relatively low,indicating that the conditions for component flow of retained hydrocarbons underground have deteriorated.The study also demonstrates that preservation conditions(preventing light hydrocarbon escape and maintaining formation energy)and production strategies(controlling production pressure differential and maintaining stable operations)are important factors in regulating the occurrence and continuity of"component flow"to maximize EUR per well.These new insights can be applied to the evaluation of economically productive"sweet spots"and provide guidance for achieving optimal EUR per well in shale oil production.
基金Supported by the Central Public-interest Scientific Institution Basal Research Fund,YSFRI,CAFS(No.20603022024016)the Central Public-interest Scientific Institution Basal Research Fund,CAFS(Nos.2023TD52,2023TD76)the earmarked fund for CARS(No.CARS-49)。
摘要Crassostrea gigas has good taste and high nutritional value;however,there are few assessments of comprehensive and panoramic analyses of the nutritional quality of the northern oyster.To study the nutritional characteristics of C.gigas from different sources(ploidy,region,size,and culture mode),C.gigas from various ploidy(diploid and triploid),regions(Rushan,Off-site fattening,and Rongcheng),sizes(small,medium,and large)and culture modes(nearshore and offshore)were selected for comparative analyses.The nutritional components(moisture,protein,fat,and mineral),flavor substances(taste amino acids,nucleotides,and succinic acid),and functional indices(eicosapentaenoic acid(EPA),docosahexaenoic acid(DHA),and taurine)of C.gigas were determined.Principal component analysis(PCA)was used to comprehensively evaluate the oysters and investigate the variations in nutritional quality.The PCA results indicate that protein,essential fatty acids,selenium,zinc,taste amino acids,taurine,EPA,and DHA were core components contributing to 82.25%of the cumulative variance,providing a more comprehensive reflection of the nutrient composition of C.gigas.The extensive quality rankings for the C.gigas were as follows:diploid>triploid,Rushan>fattening>Rongcheng,medium>large>small,and offshore>nearshore.The score rank revealed that diploid oysters of medium-size from Rushan demonstrated superior nutritional quality compared to other tested samples.This is the first comprehensive and systematic investigation of C.gigas in northern China to reveal the feature of nutrients,flavor,and functional components.The study provided data support for the culture,consumption,processing,research,and nutritional quality improvement of oyster industry.
基金We acknowledge the technical support from the National Natural Science Foundation of China(No.42307256)the Joint Funds of the Nature Science Foundation of Hubei Province(No.2022CFD172)+4 种基金the Joint Funds of the National Nature Science Foundation of China(U22A20232)the Innovation Research Group Project of the Hubei Provincial Department of Science and Technology(2025AFA020)the Open Project Funding of Hubei Key Laboratory of Environmental Geotechnology and Ecological Remediation for Lake&River(HJKFYB202405)the Hubei Provincial Department of Education's Outstanding Mid-aged and Young Technological Innovation Team(No.T2024006)the Innovation Demonstration Base of Ecological Environment Geotechnical,Ecological Restoration of Rivers and Lakes(2020EJB004)。
摘要Understanding the synergistic mechanism between root mechanical effects and soil ecological effects is essential for revealing the dynamic evolution of root reinforcement.However,how this synergy evolves with plant growth stages remains unclear.To address this,a 360-day outdoor experiment was conducted using three herbaceous species:Cynodon dactylon,Trifolium repens,and Lolium perenne.Root-soil composites,unrooted soils,and intact roots were sampled at seven intervals for the measurement of disintegration rate,shear strength,soil physicochemical properties,root morphology,and root mechanical properties.Principal component analysis(PCA)identified organic matter,tensile force,and root length density as key loading factors.The results revealed a clear stage-dependent synergy:during the early stage(30-90 days),root mechanical effects(root winding and tensile force)dominated reinforcement;during the mid-to-late stage(90-360 days),soil ecological effects(organic matter accumulation and aggregate stability)progressively strengthened and co-drove reinforcement together with root traits.This dynamic synergy explained the overall reinforcement order(L.perenne>T.repens>C.dactylon)and the distinct decline observed on day 270 due to growth cycles.Notably,L.perenne exhibited earlier establishment advantages,whereas T.repens showed greater reinforcement potential than C.dactylon during the later stages.The PCA-based Y-values(0.92-5.83)integrated both effects.It is concluded that root reinforcement evolves from a mechanically dominated phase to a mechanically-ecological synergistic phase.This stage-dependent synergy pattern provides a mechanistic basis for the dynamic evaluation of root reinforcement and for vegetation selection according to soil stabilization requirements at different growth stages.
基金the financial support from the National Natural Science Foundation of China(No.52465047)the Natural Science Foundation of Jiangxi Province,China(No.20232BAB204050)the support from Alexander von Humboldt Foundation,Germany。
摘要Titanium alloy large-scale rib-web components,known for their lightweight and highstrength properties,have the potential to substantially lighten the load-bearing components of aircraft.Isothermal Local Loading(ILL)has been recognized as an advanced technique for the integrated and less-loading forming of these components.However,material transfer in the transitional region during ILL often leads to folding defect near the die-partition line.To this end,the position of the neutral layer during ILL is calculated via the slab method,which is modified to align with the material flow in the transitional region,taking into account the multi-stage stress states under diverse boundary conditions.By utilizing this calculation model,the material transfer rate and rib-groove filling height can be ascertained.The material transfer rate serves as a criterion for predicting folding defect,and thereby an optimal billet is obtained and implemented in the ILL process.The results demonstrate that the material transfer rate is significantly decreased compared to the non-optimized billet,resulting in a non-folding component of transitional region.
基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(No.PAPD-2023-87)Jiangsu Provincial Frontier Technology R&D Program(Modern Agriculture)(No.BF2025314)+3 种基金the Modern Agricultural Industrial Technology System of Shandong Province(No.SDAIT-05)the Key Research and Development Program of Shandong Province(2021TZXD007)Youth Innovation Team Plan of Colleges and Universities of Shandong Province(2023KJ167)the Foundation for High-level Talents of Qingdao Agricultural University(6631120098).
摘要Blue light and ultraviolet-A(UV-A)radiation are critical light-quality regulators that coordinately modulate plant growth and quality in protected horticulture.However,their interactive effects on celery(Apium graveolens L.)remain inadequately characterized.In the present study,we quantified the individual and combined impacts of supplemental UV-A and blue light on the growth,biomass accumulation,photosynthetic performance,and nutritional quality of celery(cv.Dayehuang)in a plant factory with artificial lighting.Our results demonstrated that sole UV-A or blue light application significantly increased biomass,nutritional quality,and light use efficiency(LUE)of celery.Notably,combined supplementation with UV-A and blue light did not enhance chlorophyll content,biomass accumulation,or nutritional quality in celery relative to supplementation with either light quality alone.The principal component analysis(PCA)score plot exhibited clear separation among the four light treatments,with the UV-A treatment distinctly separated along the positive PC1 axis.Our findings demonstrate that UV-A and blue light regulate plant growth and quality via interactive rather than additive effects.Additionally,sole UV-A irradiation significantly improved celery growth and LUE.These findings provide a theoretical foundation for optimizing the light environment for celery under protected cultivation.
基金supported by Major Program of National Natural Science Foundation of China(U22A20188)National Science Fund for Distinguished Young Scholars(52425504).
摘要The physicochemical properties of SUS304 foil surfaces are crucial to their applications.Pulsed laser modification was applied to 30μm thick SUS304 foils to systematically investigate the influence of laser energy on surface characteristics.Through multidimensional characterization of surface morphology,three-dimensional profiles and roughness,contact angle,and chemical composition,the structure-function correlation between laser energy and the physicochemical properties of steel surface was revealed.With increasing laser energy,the surface morphology of the steel transitions from a directional rolling-marked structure to a uniform sponge-like isotropic structure,accompanied by increased peak density and an expanded interfacial area.Additionally,the chemical state on the metal surface gradually stabilizes from unstable redox reactions,forming a stable oxide layer and significantly increasing active hydroxyl groups,thereby effectively improving surface wettability.Single lap shear tests reveal an enhancement in the bonding strength of steel/carbon fiber reinforced composites joints after laser modification,which is attributed to the synergistic effects of mechanical interlocking,enhanced wettability,and chemical bonding at the interface.The demonstrated potential of laser surface treatment for modifying SUS304 ultra-thin foils provides theoretical support and technical reference for its application in fiber metal laminates.
基金funded by the National Natural Science Foundation of China(Grant No.71973027)The Fujian Provincial Department of Finance Project(Grant No.KSC22R06D)+1 种基金the Program of Research Institute of Xi Jinping Ecological Civilization,Fujian Agriculture and Forestry University(Grant No.STWMSX23-01)the Youth Backbone Training Fund of Fujian Agriculture and Forestry University(Grant No.K1523020B).
摘要Establishing a robust evaluation model for the soil erosion sensitivity index(SESI)is a fundamental prerequisite for the scientific assessment of regional soil erosion.Furthermore,understanding its spatiotemporal evolution and underlying driving mechanisms is essential for developing differentiated and refined regional optimization strategies.This study takes Fujian Province—a typical red soil erosion-vulnerable area in southern China—as a case study.Using spatial principal component analysis(SPCA)and geographical detectors,we selected seven factors(slope,relief amplitude,soil type,annual precipitation,normalized difference vegetation index(NDVI),population density,and land use type)to construct an evaluation indicator system for SESI.The spatiotemporal dynamics and influencing factors of SESI in Fujian Province were then analyzed.The results show that:(1)The spatial distribution pattern of SESI levels in Fujian Province remained relatively stable over the study period and exhibited significant spatial autocorrelation,characterized by high-high and low-low clustering.(2)SESI in Fujian Province generally transitioned from improving sensitivity along the southeastern coast to worsening sensitivity in the northwestern inland areas.(3)SESI was primarily controlled by natural background conditions,particularly topography and landform,with secondary influences from human activities.The driving effects of different factors were mainly manifested through interactive effects,with interaction types evolving from weak interaction to strong interaction,and finally to balanced interaction.
基金funded by the Deep Earth Probe and Mineral Resources Exploration-National Science and Technology Major Project(No.2024ZD1003903)the National Natural Science Foundation of China(Nos.52374078 and U24A20616)+1 种基金the Sichuan-Chongqing Science and Technology Innovation Cooperation Program Project(No.2024TIAD-CYKJCXX0011)the Fundamental Research Funds for the Central Universities(No.2023CDJKYJH021)。
摘要Rockbursts frequently occur during deep mining.They are catastrophic failures of the coal-rock composite(CRC);however,identifying their precursory signals remains challenging.This study presents a principal component analysis(PCA)-density-based spatial clustering of applications with noise(DBSCAN)-assisted workflow for warning-time analysis of CRCs.Conventional uniaxial and cyclic loading-unloading tests were conducted on CRC with four different roof rock lithologies.PCA was used to reduce redundancy among multiple acoustic emission(AE)parameters,and DBSCAN was used to organize AE samples in the reduced feature space for subsequent warning-time analysis.The results indicate an inflection point in the axial strain rate before failure,which may reflect a transition in the deformation state of the composite near the critical stage.AE signal evolution shows distinct stages,with AE count and AE energy rising sharply near failure.The PCA-DBSCAN-assisted workflow identifies different warningrelated patterns:responses in the coal body are more dispersed and appear earlier,whereas responses in the roof rock are more concentrated closer to failure.The workflow provides a structured basis for organizing correlated AE responses and comparatively analyzing warning-related evolution in the coal body and the roof rock.Importantly,warning time is affected by roof lithology and generally appears earlier in the coal body than in the roof rock.These findings provide a laboratory-scale basis for comparative warning analysis of CRCs under different lithological conditions.
基金supported by the National Natural Science Foundation of China (No. 42277487)the Science and Technology Program of Xi'an, China: Special Project of Xi'an University 'Scientific and technological personnel in universities serve enterprises' (No. 24GXFW0081– 06)
摘要Pollution of potentially harmful elements(PHEs)poses a significant challenge to an urban environment.Fine roadway dust(FRD,particle size<63μm)often accumulates elevated concentrations of PHEs,posing a threat to the ecological environment and human health,while also curbing urban sustainable development.In this study,a source–receptor model combined with one-dimensional Monte Carlo simulation(1-D MCS)and two-dimensional Monte Carlo simulation(2-D MCS)was used to assess the probabilistic eco-health risks of PHEs in FRD from Xingtai,a representative industrial city in northern China.The average concentrations of Ba,Cr,Cu,Mn,Ni,Pb,V,and Zn exceeded the background values of the local soils,and the overall degree of contamination was moderate.The PHEs originated from traffic,industrial,and natural sources,accounting for 44.2%,37.8%,and 18.0%of the total sources of PHEs,respectively.The overall eco-risk of PHEs was relatively low,whereas PHEs could pose a certain non-carcinogenic risk to children.The cancer risks to all populations,especially children,cannot be ignored.Cr was a priority pollutant,and industrial sources were the primary control targets.This study recommended the threshold values of exposure concentrations using the 2-D MCS model.The results showed that the cancer risk to children exceeded the safety threshold at Cr levels higher than 62.67 mg/kg,and Pb could pose a potential ecological risk to the environment at concentrations reaching 174.99 mg/kg.This study provides scientific guidance for pollution control and risk management of PHEs in industrial cities worldwide.
基金supported by the Jilin Province Health Science and Technology Ability Improvement Project(2023JL057).
摘要Objective:Triple-negative breast cancer(TNBC)is highly aggressive and lacks an effective targeted therapy.This study aimed to elucidate the functions and possible mechanisms of action of zinc finger miz-type containing 2(ZMIZ2)and minichromosome maintenance complex component 3(MCM3)in TNBC progression.Methods:The relationship between ZMIZ2 expression and clinical characteristics of TNBC was investigated.In vitro and in vivo experiments were performed to investigate the role of ZMIZ2 dysregulation in TNBC cell malignant behaviors.The regulatory relationship between ZMIZ2 and MCM3 was also explored.Transcriptome sequencing was performed to elucidate possible mechanisms underlying the ZMIZ2/MCM3 axis in TNBC.Results:High ZMIZ2 expression levels were associated with the malignant degree of TNBC.ZMIZ2 overexpression promoted TNBC cell proliferation,migration,and invasion;inhibited apoptosis;and induced G1 phase cell cycle arrest,whereas knockdown of ZMIZ2 had the opposite effect.ZMIZ2 directly targeted and positively regulated MCM3 expression.MCM3 knockdown reversed the effect of ZMIZ2 overexpression on TNBC tumor growth both in vitro and in vivo.High MCM3 expression levels were linked to the degree of malignancy and poor prognosis in TNBC.The differentially expressed genes associated with the ZMIZ2/MCM3 axis were significantly enriched in multiple pathways,such as the mitogen-activated protein kinase(MAPK),mechanistic target of rapamycin(mTOR),Wnt,and Ras signaling pathways,as verified by The Cancer Genome Atlas data.Conclusions:ZMIZ2 and MCM3 were highly expressed in TNBC.ZMIZ2 promoted the development by positively regulating MCM3 expression.Key pathways,such as the Ras/MAPK,phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mTOR,and Wnt signaling pathways,may be key downstreammechanisms.
基金funded by the National Natural Science Foundation of China(22204038)the Fundamental Research Funds for the Central Universities(JZ2024HGTB0231)the National Natural Science Foundation of China(22073078,12075072,and 12275228).
摘要Benefitting from the non-invasive and quantitative detection property,liquid NMR spectroscopy shows broad applications in the identification,separation,and structural determination of chemical substances in mixtures.However,its applications,especially to probing complex mixtures that contain abundant components and complicated molecular structures,somewhat encounter the challenge of spectral congestion.In this study,we demonstrate the ultra-selective extraction of targeted components from overlapped NMR spectra for mixture analyses by investigating the applications of the recent 1D selective GEMSTONE-TOCSY approach.This strategy first adopts an ultra-selective chemical shift filter to choose the targeted spins related to the components of interest,even though situated at overlapped spectral regions with decent suppression on unwanted surrounding signals.Sequentially,it utilizes the 1D selective TOCSY scheme to accurately extract total coupling networks associated with targeted spins,and then spectroscopically separate individual components,thus resolving overlapped NMR spectra.Apart from the measurements on electrolyte and sugar mixtures,experimental results demonstrate that it also allows one to analyze heterogeneous biological tissues containing plentiful metabolites and intrinsic field inhomogeneity via specific probe access to targeted components.Therefore,this study demonstrates a useful tool for component analyses and molecular structure measurements on complex chemical and biological mixtures,and it shows promising prospects for wide applications in the fields of chemistry,biology,energy,etc.