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Degradation of beechwood xylan using food-grade bacteria-like particles displayingβ-xylosidase from Limosilactobacillus fermentum 认领 引用
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作者 Robie Vasquez Ji Hoon Song +4 位作者 Jae Seung Lee Bernadette Bagon Sanghoon Kim Valerie Diane Valeriano Dae-Kyung Kang 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期1075-1088,共14页
The display of enzymes on bacterial surfaces is an interesting approach for immobilising industrially important biocatalysts.In recent years,non-recombinant surface display using food-grade bacteria,such as lactic aci... The display of enzymes on bacterial surfaces is an interesting approach for immobilising industrially important biocatalysts.In recent years,non-recombinant surface display using food-grade bacteria,such as lactic acid bacteria(LAB),have gained interest because of their safety,simplicity,and cost-effectiveness.β-Xylosidase is one of the many biocatalytic enzymes targeted for immobilisation due to its key role in the complete saccharification of lignocellulosic biomass,including xylan hemicellulose.Recently,the xylose-tolerantβ-xylosidase,LfXyl43,was identified in Limosilactobacillus fermentum.LfXyl43 is capable of producing xylose from the degradation of xylo-oligosaccharides(XOS)and beechwood xylan.This study aimed to immobilise this new biocatalyst on the surface of LAB-derived bacteria-like particles(BLP)and investigate its applicability and reusability in the degradation of xylan hemicellulose.Additionally,the influence of the anchor position and the presence of linker peptides on the display and activity of theβ-xylosidase was investigated.Four expression vectors were constructed to express different anchor-xylosidase fusion proteins.Upon expression and purification,all anchor-xylosidase fusion proteins were active towards the artificial substrate p-nitrophenyl-β-D-xylopyranoside.In addition,all anchor-xylosidase fusion proteins were successfully displayed on the surface of BLP.However,only theβ-xylosidases with linker peptide showed hydrolytic activity after immobilisation on BLP.BLP displayingβ-xylosidases demonstrated high activity against XOS and beechwood xylan,thereby producing high amounts of xylose.Moreover,the immobilised enzyme demonstrated reusability across several bioconversion cycles.Overall,this study highlights the potential industrial application of surface-displayedβ-xylosidase for the effective degradation of lignocellulosic biomass. 展开更多
关键词 Surface display Beta-xylosidase Lactic acid bacteria Xylan Immobilisation Biocatalyst
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Application of an M13 bacteriophage displaying tyrosine on the surface for detection of Fe3+ and Fe2+ ions 认领 引用
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作者 Xiaohua Guo Chuncheng Niu +1 位作者 Yunhua Wu Xiaosheng Liang 《Virologica Sinica》 SCIE CAS CSCD 2015年第6期410-416,共7页
Ferric and ferrous ion plays critical roles in bioprocesses,their influences in many fields have not been fully explored due to the lack of methods for quantification of ferric and ferrous ions in biological system or... Ferric and ferrous ion plays critical roles in bioprocesses,their influences in many fields have not been fully explored due to the lack of methods for quantification of ferric and ferrous ions in biological system or complex matrix.In this study,an M13 bacteriophage(phage) was engineered for use as a sensor for ferric and ferrous ions via the display of a tyrosine residue on the P8 coat protein.The interaction between the specific phenol group of tyrosine and Fe3+./ Fe2+.was used as the sensor.Transmission electron microscopy showed aggregation of the tyrosine-displaying phages after incubation with Fe3+ and Fe2+.The aggregated phages infected the host bacterium inefficiently.This phenomenon could be utilized for detection of ferric and ferrous ions.For ferric ions,a calibration curve ranging from 200 nmol/L to 8 μmol/L with a detection limit of 58 nmol/L was acquired.For ferrous ions,a calibration curve ranging from 800 nmol/L to 8μmol/L with a detection limit of 641.7 nmol/L was acquired.The assay was specific for Fe(3+) and Fe(2+) when tested against Ni2+,Pb2+,Zn2+,Mn2+,Co2+,Ca2+,Cu2+,Cr3+,Ba2+,and K^+.The tyrosine displaying phage to Fe3+ and Fe2+ interaction would have plenty of room in application to biomatenals and bionanotechnology. 展开更多
关键词 M13 bacteriophage tyrosine display ferric ion ferrous ion aggregation
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Cost effective filamentous phage based immunization nanoparticles displaying a full-length hepatitis B virus surface antigen 认领 引用
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作者 Bertan Koray Balcioglu Aylin Ozdemir-Bahadir +2 位作者 Duygu Hinc Candan Tamerler Berrin Erdag 《Advances in Bioscience and Biotechnology》 CAS 2014年第1期46-53,共8页
Hepatitis B virus (HBV) is one of the major causes of chronic hepatitis, cirrhosis and liver cancer. In combating HBV infections, HBV diagnosis and vaccination are therefore critical. The hepatitis B virus surface ant... Hepatitis B virus (HBV) is one of the major causes of chronic hepatitis, cirrhosis and liver cancer. In combating HBV infections, HBV diagnosis and vaccination are therefore critical. The hepatitis B virus surface antigen (HBsAg) is a key target molecule in developing vaccines and diagnostic systems. To date, although HBsAg has been expressed in bacteria, yeasts and mammalian cells, there are still limitations in the existing ones, which leave the necessity for searching new HBsAg production methods. In this study, a simple phage display-based method was developed to produce the purified full-length HBsAg molecules for further immunization studies. For this purpose, the HBsAg coding gene was cloned into a pCANTAB5E phagemid vector and expressed on the surface of M13 filamentous phages. The HBsAg-expressing phage nanosystem was then used as immunization agent in BALB/cJ mice. The ELISA results for sera obtained from mice immunized with HBsAg-displaying phage particles revealed an immune response against HBsAg. These results demonstrate the potential use of a full-length antigen to be displayed on phages as cost effective adjuvant-free immunization agents as an alternative to the highly purified and more expensive antigens conjugated with carrier molecules. 展开更多
关键词 Phage Display Hepatitis B Virus Surface Antigen Protein Expression Phage Immunization Nano Vector System
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CPL-enabled spatial displaying for immersive human-machine interaction 认领 引用 被引量：1
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作者 Zhiyong Tang 《Science China Materials》 SCIE EI CAS CSCD 2025年第12期4586-4587,共2页
Three-dimensional(3D) display technology—a cutting-edge medium for human-machine interaction—enhances visual information density via image dimensional expansion and reduces the cognitive load to improve the efficien... Three-dimensional(3D) display technology—a cutting-edge medium for human-machine interaction—enhances visual information density via image dimensional expansion and reduces the cognitive load to improve the efficiency of information exchange [1–3]. 展开更多
关键词 immersive human machine interaction three dimensional display technology cognitive load visual information density image dimensional expansion cpl enabled spatial displaying reduces cognitive load improve efficiency information exchange
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Integrated metasurface-freeform system enabled multi-focal planes augmented reality display 认领 引用 被引量：3
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作者 Shifei Zhang Lina Gao +9 位作者 Yidan Zhao Yongdong Wang Bo Wang Junjie Li Jiaxi Duan Dewen Cheng Cheng-Wei Qiu Yongtian Wang Tong Yang Lingling Huang 《Opto-Electronic Science》 CAS 2026年第1期1-12,共12页
The advent of artificial intelligence(AI)has propelled augmented reality(AR)display technology to a pivotal juncture,positioning it as a contender for the next generation of mobile intelligent terminals.However,the pu... The advent of artificial intelligence(AI)has propelled augmented reality(AR)display technology to a pivotal juncture,positioning it as a contender for the next generation of mobile intelligent terminals.However,the pursuit of advanced AR displays,particularly those capable of delivering immersive 3D experiences,is significantly hindered by the performance limitations of current hardware and the complexity of system integration.In this study,we present an innovative multi-focal plane AR display system that integrates a non-orthogonal polarization-multiplexing metasurface,freeform optical elements,and an OLED display screen.All optical elements are integrated into a single solid-state architecture,based on a joint optimization design approach of ray tracing and diffraction theory.The multi-focal plane AR visual effect is realized by the compact and multiplexing metasurface,which performs distinct phase functions across diverse polarization channels.Meanwhile,freeform surfaces offer ample design flexibility for the collaborative optimization of multi-focal plane imaging and the see-through systems.Followed by a mechanical design and prototype assembly,we demonstrate the system's capabilities in real-time and multi-focal plane display.The digital images at all virtual image distances seamlessly integrate with the real environment,fully exhibiting the system's high parallelism and real-time interactivity.With the innovative design concept and joint design method,we believe that our work will spur more innovative and compact intelligent solutions for AR displays and inject new vitality into hybrid optical systems. 展开更多
关键词 augmented reality metasurface-freeform multi-focal planes display non-orthogonal polarizationmultiplexing metasurfaces
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Active retinal projection augmented reality display via pixel-to-pixel collimation 认领 引用 被引量：1
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作者 Xiang Zhang Yuanlong Huang +2 位作者 Weiyao Fan Enguo Chen Jiajun Luo 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2026年第5期14-23,共10页
As the next-generation human–computer interface,augmented reality display technology has been gradually popular,depending on advances in system architectures.However,existing waveguide and passive retinal projection ... As the next-generation human–computer interface,augmented reality display technology has been gradually popular,depending on advances in system architectures.However,existing waveguide and passive retinal projection display solutions are unable to combine their respective advantages to address increasingly demanding performance requirements.Herein,we propose an active retinal projection display(A-RPD)concept based on collimated active-matrix microdisplay panels.By deriving and validating the collimation-dependent depth of focus,the optimization direction of this architecture is emphasized.Through the direct integration of pixel-to-pixel collimators on the microdisplay panels,an A-RPD prototype featuring a balanced design and performance has been successfully constructed.It enables clear retinal imaging from 40 cm to 160 cm,which significantly surpasses that of the uncollimated microdisplay.The proposed active retinal projection architecture retains the advantages of retinal projection while simplifying the architecture.This work highlights its importance and superiority and provides foundations for its further expansion in practical applications. 展开更多
关键词 retinal projection display augmented reality display optical design display
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Bionic femtosecond laser manufacturing for impressionistic camouflage infrared display 认领 引用 被引量：2
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作者 Jianing Liao Zhuguo Li Dongshi Zhang 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2026年第1期627-653,共27页
White Cyphochilus insulanus beetles,exhibiting both environmental camouflage display and radiative cooling functions,serve as a good prototype for biomimetic fabrication.As inspired,this work presents a femtosecond(fs... White Cyphochilus insulanus beetles,exhibiting both environmental camouflage display and radiative cooling functions,serve as a good prototype for biomimetic fabrication.As inspired,this work presents a femtosecond(fs)laser-based biomimetic fabrication strategy that takes full use of the synthesized radiative cooling nanomaterials for a groundbreaking stimuli-responsive infrared(IR)impressionistic camouflage display.The proposed technique is capable of readily transforming various substrates(quartz glass and metals including Ti,Al,Zr,and W)into self-assembled porous networks(aerogels)consisting of oxygen-vacancy-rich oxide nanoparticles.Surprisingly,the emissions of all as-prepared porous particle-networks in the radiative-cooling long-wavelength infrared(LWIR)band are above 95%,with the SiO2 aerogels reaching a maximum of 99.6%.Benefiting from the far-from-equilibrium thermodynamic kinetics,metastable phases of anatase TiO2,tetragonal zirconia(t-ZrO2),and monoclinic WO3(Pc)are synthesizable,opening up opportunities for exploring their optical applications.Taking the low-temperature metastable phase WO3(Pc)as representative for systematic studies,it is found that(1)the ratio WO3(Pc)phase to that of room-temperature phase of WO3(P21)can be tailored by modulation of processing parameters;(2)laser synthesized aerogels with hybrid phases of WO3(Pc)and WO3(P21)have a brighter visible whiteness,higher visibleearinfrared(NIR)spectral selectivity than the natural prototype of white Cyphochilus insulanus beetles but with comparable LWIR emittance.White WO3 aerogel in situ deposited during flexibly fs laser artistic patterning can blur the painting features due to its radiative cooling effect,allowing a colorful impressionistic IR display in the heating mode.What's more,invisible painting features concealed by the white deposited WO3 aerogel are clearly/faintly distinguishable by introducing external stimuli of a human hand and sample heating,respectively,catalyzing progress in optical encryption and selectively stimuli-responsive decryption display in the infrared band. 展开更多
关键词 femtosecond laser bionic manufacturing metastable phase impressionistic infrared display infrared camouflage
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Isolation of neutralizing antibodies against SARS-CoV-2 through an epitope-guided negative screening by phage display 认领 引用 被引量：1
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作者 Ming Lu Yin Chen +6 位作者 Xiaoyu Liu Fang Gao Liming Gou Wei Ye Jiaqi Wen Xiling Guo Wei Gao 《Journal of Biomedical Research》 CAS CSCD 2026年第2期210-223,共14页
Neutralizing antibodies are essential tools in antiviral therapy and epidemic preparedness,capable of directlyinhibiting viral entry and limiting disease progression.However,traditional antibody discovery strategies—... Neutralizing antibodies are essential tools in antiviral therapy and epidemic preparedness,capable of directlyinhibiting viral entry and limiting disease progression.However,traditional antibody discovery strategies—suchas animal immunization or B cell isolation from infected individuals—are often hindered by biosafety concerns,lengthy development timelines,and limited adaptability during outbreaks.In the present study,we aimed toestablish a robust and rapid in vitro platform for the efficient isolation of neutralizing antibodies targetingconserved viral epitopes.We developed an epitope-guided negative screening strategy that integrates phagedisplay technology with rational antigen mutagenesis to exclude antibodies against variable regions whileenriching for those that recognize functionally constrained epitopes.When applied to the receptor-binding domainof severe acute respiratory syndrome coronavirus 2,this method enabled the identification of six neutralizingantibodies(one IgG and five nanobodies)exhibiting broad-spectrum neutralizing activity across multiple viralvariants.Notably,antibodies recognizing distinct epitopes demonstrated significant synergistic neutralizationwhen used in combination(P<0.05).This screening approach facilitates the rapid discovery of potent andmutation-resistant antibodies and holds promise for application to other emerging pathogens.Our findingsunderscore the potential of epitope-guided,in vitro platforms in expediting therapeutic antibody developmentunder conditions of high biosafety requirements. 展开更多
关键词 epitope-guided neutralizing antibody SARS-CoV-2 phage display
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Zn Anode-Based Electrochromic Devices:Progress&Challenges 认领 引用 被引量：1
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作者 Bingkun Huang Feifei Zhao +6 位作者 Pengcheng Liu Yukai Xu Bin Wang Jiaqi Yang Jingwei Chen Haizeng Li William W.Yu 《Energy & Environmental Materials》 SCIE EI CAS CSCD 2026年第1期15-33,共19页
Unlike conventional electrochromic devices,Zinc anode-based electrochromic devices(ZECDs)ensure excellent charge balance between the electrochromic layer and Zn anode during the coloring/bleaching by reversible metal ... Unlike conventional electrochromic devices,Zinc anode-based electrochromic devices(ZECDs)ensure excellent charge balance between the electrochromic layer and Zn anode during the coloring/bleaching by reversible metal deposition/stripping on the Zn anode.Meanwhile,the inherent potential difference between the metal anode and the electrochromic layer can drive the spontaneous coloration/bleaching of ZECDs,featuring energy retrieval functionality.This review discusses the working mechanisms,performance indexes of ZECDs,and the impact of material selection on ZECD performance.Furthermore,we comprehensively summarize the latest research progress of ZECDs in energy storage,smart windows,and multicolor displays.We argue that using high-transparency zinc mesh,additive manufacturing processes,and self-healing electrochromic materials can significantly advance the commercialization of large-area ZECDs.Finally,“electrode-free”device structures,renewable or replaceable electrolytes,and strategies to suppress zinc dendrites are prospected to overcome cost-effectiveness and lifespan issues of ZECDs.This review aims at enabling more efficient and advanced ZECDs for multifunctional applications. 展开更多
关键词 electrochromic devices energy storage multicolor displays smart windows zinc anode
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Unity-based virtual reality for detector and event visualization in JUNO experiment 认领 引用
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作者 Kai-Xuan Huang Tian-Zi Song +4 位作者 Yu-Ning Su Cheng-Xin Wu Xue-Sen Wang Yu-Mei Zhang Zheng-Yun You 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2026年第4期127-138,共12页
Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effect... Detector and event visualization are crucial components of high-energy physics(HEP)experimental software.Virtual reality(VR)technologies and multimedia development platforms,such as Unity,offer enhanced display effects and flexible extensibility for visualization in HEP experiments.In this study,we present a VR-based method for detector and event displays in the Jiangmen Underground Neutrino Observatory(JUNO)experiment.This method shares the same detector geometry descriptions and event data model as those in the offline software and provides the necessary data conversion interfaces.The VR methodology facilitates an immersive exploration of the virtual environment in JUNO,enabling users to investigate the detector geometry,visualize event data,and tune the detector simulation and event reconstruction algorithms.Additionally,this approach supports applications in data monitoring,physics data analysis,and public outreach initiatives. 展开更多
关键词 Virtual reality Event display Unity Detector geometry JUNO
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Diquafosol ophthalmic solution on ocular surface parameters in visual display terminal-associated dry eye disease 认领 引用
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作者 Ting-Ting Yang Hong-Yu Duan +7 位作者 Yi-Fan Zhou Bai-Kai Ma Lu Zhao Qian-Qian Lan Jia-Wei Chen Xiao-Yu Wang Kyung Chul Yoon Hong Qi 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2026年第4期673-680,共8页
AIM:To evaluate 3%diquafosol ophthalmic solution on ocular surface parameters and the alterations of lipid and muco-aqueous layer in tear film of patients with visual display terminal(VDT)-associated dry eye disease(D... AIM:To evaluate 3%diquafosol ophthalmic solution on ocular surface parameters and the alterations of lipid and muco-aqueous layer in tear film of patients with visual display terminal(VDT)-associated dry eye disease(DED).METHODS:This study included patients with VDTassociated DED.It was a prospective single-arm interventional clinical trial.Patients were provided with 3%diquafosol ophthalmic solution for 3mo and were followed up in 1,2 and 3mo after treatment.Tear breakup time(TBUT),ocular surface staining score,and ocular surface disease index(OSDI)score were ocular surface characteristics.Lipid layer thickness(LLT),tear meniscus height(TMH),and mucin mRNA expression levels(MUC1,MUC4,MUC5AC,MUC16,and MUC20)were used to measure changes in the tear film.The LipiView interferometer was used to measure the partial blink rate(PBR).RESULTS:Sixty-eight eyes of 68 participants(54 females;mean age 25.12±4.10y;mean spherical equivalent-4.35±2.69 D)were enrolled.Compared with the pre-treatment,OSDI scores and TBUT improved significantly at all follow-up time points(all P<0.01),and TMH increased significantly at 1 and 3mo(P<0.01,P<0.001,respectively).Conjunctival lissamine green staining improved only at 2mo(P<0.05),while corneal fluorescein staining showed no significant changes.Overall LLT remained unchanged,but the PBR<1 subgroup exhibited significant LLT elevation at 3mo(P<0.05),unlike the PBR=1 subgroup.Conjunctival mRNA expression of MUC1,MUC5AC,MUC16,and MUC20 was significantly upregulated at 1 and 3mo(all P<0.01),and MUC4 expression increased significantly only at 1mo(P<0.001).CONCLUSION:In patients with VDT-associated DED,3%diquafosol ophthalmic solution dramatically reduced symptoms and enhanced tear film stability by promoting ocular surface muco-aqueous secretion.Patients with better blinking habits(PBR<1)demonstrate greater LLT improvement than those with poorer habits. 展开更多
关键词 dry eye disease visual display terminal diquafosol mucin
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Tunable color display and efficient thermal regulation with grating colored radiative cooler 认领 引用
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作者 Chunzhen Fan Cong Ren Hengli Xie 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第6期757-765,共9页
Integrating color display with radiative cooling is of great importance for applications in both aesthetic appeal and thermal management.However,current colored radiative coolers primarily rely on geometric modulation... Integrating color display with radiative cooling is of great importance for applications in both aesthetic appeal and thermal management.However,current colored radiative coolers primarily rely on geometric modulation,resulting in monochromatic color and limiting their applicability.Here,we present a tunable colored radiative cooler(GCRC)comprising an upper grating emitter and a lower grating reflector.The emitter achieves a high average emissivity of 98.8%and 86.5%within the first and second atmospheric transparency windows simultaneously,and it demonstrates angularinsensitive emissivity(>70%)across 0–90°incident angle.Meanwhile,the grating reflector enables dynamic color manipulation through variations in polarization angle,dielectric layer thickness,and grating filling fraction.Notably,the GCRC demonstrates a maximum net nighttime cooling power of 232.08 W·mm-2at 300 K,and it reaches up to 74.6 W·m-2under solar irradiation.Thus,our design not only delivers vibrant,adjustable color but also outperforms conventional radiative coolers in terms of cooling efficiency,making it a promising solution for architectural coatings,smart aesthetics,and advanced thermal management systems. 展开更多
关键词 grating radiative cooling color display emitter reflector
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Sustainable biopolymer hydrogel electrolytes for electrochromics:Materials,mechanisms,and roadmaps to next-generation smart technologies 认领 引用
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作者 Pramod V.Rathod Pooja V.Chavan Hern Kim 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2026年第4期660-682,I0016,共23页
Biopolymer-based hydrogel electrolytes are rapidly emerging as sustainable alternatives for electrochromic devices(ECDs)due to their environmental compatibility,tunable ionic conductivity,and mechanical flexibility.Re... Biopolymer-based hydrogel electrolytes are rapidly emerging as sustainable alternatives for electrochromic devices(ECDs)due to their environmental compatibility,tunable ionic conductivity,and mechanical flexibility.Recent advances leverage renewable biopolymers such as cellulose,chitosan,and alginate,along with their nanostructured derivatives,to create dynamic hydrogel matrices with optimized ionic transport and multifunctional properties.This review comprehensively details the evolution of biopolymer hydrogel electrolytes in ECDs by emphasizing structure-performance relationships,including molecular design,crosslinking chemistry,and interfacial engineering for enhanced electro-optical properties.However,despite notable progress,several critical bottlenecks remain:limited long-term operational stability under varying humidity and temperature,susceptibility to water evaporation and drying,narrow electrochemical voltage windows,and challenges in scaling for large-area device integration.These issues currently hinder their widespread deployment relative to synthetic counterparts.We critically analyze recent strategies to overcome these limitations,such as scalable fabrication techniques and adaptive encapsulation methods,aiming to accelerate material innovation and device performance optimization.Furthermore,the review highlights the expanding application scope of biopolymer-based ECDs,including adaptive smart windows,wearable biosensors,and sustainable display technologies.By integrating achievements with current challenges,this work offers a balanced roadmap toward durable,high-performance,and environmentally responsible electrochromic systems based on next-generation biopolymer hydrogels. 展开更多
关键词 Biopolymer electrolyte Sustainable electrochromic device Energy-efficient displays Wearable optoelectronics
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A MAP OF LOCAL PATTERN ANALOGUE COEFFICIENT——A TOOL FOR DISPLAYING CIRCULATION ANOMALY 认领 引用 被引量：6
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作者 王盘兴 《Acta meteorologica Sinica》 1992年第3期325-331,共7页
Limitations of difference maps showing circulation anomalies are analyzed, and the definition of the local pattern analogue coefficient (LPAC) is given together with the procedure for constructing such a map, followed... Limitations of difference maps showing circulation anomalies are analyzed, and the definition of the local pattern analogue coefficient (LPAC) is given together with the procedure for constructing such a map, followed by an example illustrating its useful application in circulation anomaly. 展开更多
关键词 LPAC (local pattern analogue coefficient) circulation anomaly displaying tool
Hybrid metasurfaces-freeform optics bring multi-depth AR close to reality 认领 引用
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作者 Daping Chu 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2026年第5期1-2,共2页
Achieving realistic depth perception in augmented reality(AR)displays remains a central challenge due to the reliance on single focal planes.In the recent work published in Opto-Electronic Science,researchers from Bei... Achieving realistic depth perception in augmented reality(AR)displays remains a central challenge due to the reliance on single focal planes.In the recent work published in Opto-Electronic Science,researchers from Beijing Institute of Technology and their collaborators report a hybrid metasurface-freeform optical architecture that enables simultaneous multi-focal plane display within a compact,solid-state system.By combining polarizationmultiplexed metasurfaces with freeform optics through a joint design framework,the system generates multiple depth cues without time multiplexing and remains compatible with practical display hardware.This work highlights a promising route toward cross-scale optical co-design for realistic near-eye display systems. 展开更多
关键词 multi-depth AR display metasurface-freeform joint design non-orthogonal polarization multiplexing
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Understanding and Detecting Inefficient Image Displaying Issues in Android Apps 认领 引用
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作者 李文杰 马骏 +2 位作者 蒋炎岩 许畅 马晓星 《Journal of Computer Science & Technology》 SCIE EI CSCD 2024年第2期434-459,共26页
Mobile applications(apps for short)often need to display images.However,inefficient image displaying(IID)issues are pervasive in mobile apps,and can severely impact app performance and user experience.This paper first... Mobile applications(apps for short)often need to display images.However,inefficient image displaying(IID)issues are pervasive in mobile apps,and can severely impact app performance and user experience.This paper first establishes a descriptive framework for the image displaying procedures of IID issues.Based on the descriptive framework,we conduct an empirical study of 216 real-world IID issues collected from 243 popular open-source Android apps to validate the presence and severity of IID issues,and then shed light on these issues’characteristics to support research on effective issue detection.With the findings of this study,we propose a static IID issue detection tool TAPIR and evaluate it with 243 real-world Android apps.Encouragingly,49 and 64 previously-unknown IID issues in two different versions of 16 apps reported by TAPIR are manually confirmed as true positives,respectively,and 16 previously-unknown IID issues reported by TAPIR have been confirmed by developers and 13 have been fixed.Then,we further evaluate the performance impact of these detected IID issues and the performance improvement if they are fixed.The results demonstrate that the IID issues detected by TAPIR indeed cause significant performance degradation,which further show the effectiveness and efficiency of TAPIR. 展开更多
关键词 Android application(app) inefficient image displaying(IID) performance empirical study static analysis
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Assembly and integration of micro-led displays:a review of transfer methods targeting near-perfect yield 认领 引用 被引量：1
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作者 Seong Woo Hong Hyeon Ho Shin +5 位作者 Seol Ahn Hyeon Ji Yang Kyung Jun Lee Myungwon Lee Ji Woong Cho Yei Hwan Jung 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2026年第2期284-314,共31页
Micro-light-emitting diodes(micro-LEDs)are widely recognized for their superior brightness,efficiency,and durability,offering transformative potential for next-generation displays and emerging applications.However,the... Micro-light-emitting diodes(micro-LEDs)are widely recognized for their superior brightness,efficiency,and durability,offering transformative potential for next-generation displays and emerging applications.However,the path to commercialization remains hindered by a critical barrier:achieving an extremely high production yield.Unlike conventional displays,micro-LED displays require the precise transfer of millions of individual micro-LED chips,even the slightest defects can significantly affect the overall yield.This review focuses on the technological challenges of pushing micro-LED assembly yield to extremely high thresholds of 99.99999%.We explore six key transfer methods—elastomeric transfer,roll-to-roll printing,electrostatic and electromagnetic assembly,laser transfer,microvacuum assembly,and fluidic self-assembly—and analyze their respective impacts on yield.Recent advancements in each method are discussed,with an emphasis on strategies to overcome yield challenges.By framing yield as the central metric and not as a side concern,this review aims to provide a roadmap for overcoming bottlenecks in micro-LED assembly and enabling industrial-scale deployment. 展开更多
关键词 assembly integration micro-led displays transfer method yield
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Optimized anchor and cell wall engineering boost UDP-glycosyltransferase display for glycyrrhetinic acid glycosylation in Komagataella phaffii 认领 引用
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作者 Weijie Cao Jing Wang +2 位作者 Xudong Feng Hu Liu Chun Li 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2026年第3期60-68,共9页
The glycosylation of natural products like glycyrrhetinic acid(GA)is critical for improving their pharmaceutical properties.However,the industrial application of uridine diphosphate-glycosyltransferases(UGTs)is hinder... The glycosylation of natural products like glycyrrhetinic acid(GA)is critical for improving their pharmaceutical properties.However,the industrial application of uridine diphosphate-glycosyltransferases(UGTs)is hindered by their low stability and difficult recovery.Microbial surface display addresses these issues but faces a key bottleneck in achieving high display efficiency and robustness,primarily dependent on the anchoring system.This study presents a systematic engineering approach to optimize the display of UGT on the surface of Komagataella phaffii(K.phaffii)for enhanced biocatalytic performance in GA glycosylation.Initial attempts using the K.phaffii-derived anchored protein GCW51 revealed low display efficiency,with only 2852 enzymes per cell and 8.1μmol·L-1 GA-3-O-Glc production,which severely limited its industrial application potential.To address this,we expanded screening to five additional anchor proteins,and structural characterization via fluorescence microscope,flow cytometry,and TEM confirmed successful surface localization of UGT in all engineered strains,with distinct differences in display efficiency.Among them,the GPI-anchored Agαdemonstrated superior performance,achieving 8379 enzymes per cell(2.9-fold higher than GCW51)and 26.8μmol·L-1 GA-3-O-Glc production(3.3-fold improvement over GCW51).All displayed systems exhibited significantly improved thermal stability,with Agα-mediated display showing 3.0-fold longer half-life at 50℃ than free enzyme and 1.2-fold longer than GCW51.To further enhance performance,we implementedβ-glucan biosynthesis pathway engineering,yielding strain UGT-A-OE-3 with 11369 enzymes per cell(4.0-fold increase over GCW51)and 53% higher β-glucan content.The optimized system achieved 34.9μmol·L-1 GA-3-O-Glc production,representing a 4.3-fold improvement over the initial GCW51 system and 30% greater activity than the parental Agαstrain.This work establishes an effective dual-engineering strategy combining anchor optimization with metabolic pathway enhancement,significantly elevating display efficiency.The developed platform shows significant potential for industrial biocatalysis applications,particularly in glycosylation reactions requiring robust and reusable enzyme systems. 展开更多
关键词 Yeast surface display Biocatalysis Catalyst support Protein stability Synthetic biology Immobilization
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Monolithic integration of sub-volt memristor-driven pixels for capacitorless active-matrix micro-LED displays 认领 引用
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作者 Ho Jin Lee Seok Hee Hong +4 位作者 Kyung Rock Son Sim Hun Yuk Sung Keun Choi Tukaram D Dongale Tae Geun Kim 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2026年第2期742-755,共14页
Active-matrix(AM)micro light-emitting diode(micro-LED)displays rely on transistors and capacitors,thereby limiting integration density,power efficiency,and manufacturing simplicity.While the monolithic integration of ... Active-matrix(AM)micro light-emitting diode(micro-LED)displays rely on transistors and capacitors,thereby limiting integration density,power efficiency,and manufacturing simplicity.While the monolithic integration of transistors onto micro-LED chips or complementary metal oxide semiconductor-based driving circuits has been investigated,these approaches still face challenges such as complex processing,unstable transistor performance,and dependence on capacitor-integrated thin-film transistor(TFT)backplanes.In this study,we present a capacitorless AM micro-LED display architecture driven by a germanium telluride memristor(GeTe memristor),monolithically integrated with the micro-LED chip.The GeTe memristor demonstrates multilevel switching,strong drive capability,and ultra-low operating voltages(SET−0.2 V)along with excellent thermal and electrical stability.Notably,its fabrication requires no thermal annealing,thus simplifying array-level integration compared to conventional TFT-based systems.Using the aforementioned architecture,we successfully demonstrated a capacitorless 12×12 AM micro-LED array capable of displaying alphabetic characters.This approach simplifies the manufacturing process and improves pixel density.Hence,the energy-efficient GeTe memristor offers a promising alternative to conventional TFT-capacitor configurations,thereby enabling the development of low-power,high-resolution display systems. 展开更多
关键词 capacitorless active-matrix display memristor micro light-emitting diodes monolithic integration high-resolution low-power consumption
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An event visualization software based on Phoenix for the CEPC experiment 认领 引用
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作者 Yu‑Jie Zeng Tian‑Zi Song +3 位作者 Xue‑Sen Wang Yu‑Mei Zhang Tao Lin Zheng‑Yun You 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2026年第7期68-77,共10页
In high-energy physics(HEP)experiments,visualization software plays a pivotal role in detector design,offline software development,and event data analysis.The visualization tools integrate detailed detector geometries... In high-energy physics(HEP)experiments,visualization software plays a pivotal role in detector design,offline software development,and event data analysis.The visualization tools integrate detailed detector geometries with complex event data models,providing researchers with invaluable insights into experimental results.Phoenix is an emerging general-purpose visualization platform for current and next-generation HEP experiments.In this study,we developed an event display software based on Phoenix for the CEPC experiment.It offers the necessary functionalities for visualizing detector geometries and displaying event data,allowing researchers to optimize detector design,test simulation and reconstruction algorithms,and analyze event data in a visualized manner.Additionally,we discuss the future applications of the event display software,including its use in online monitoring and the potential to build virtual reality projects for enhanced data visualization. 展开更多
关键词 Visualization Event display Phoenix CEPC
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