Elevated atmospheric[CO2]and nitrogen(N)availability are critical determinants of plant growth.This study investigated the underlying mechanisms of hormones in mediating elevated[CO2]-promoted shoot growth and l...Elevated atmospheric[CO2]and nitrogen(N)availability are critical determinants of plant growth.This study investigated the underlying mechanisms of hormones in mediating elevated[CO2]-promoted shoot growth and leaf elongation under different N conditions in tall fescue(Festuca arundinacea).Plants were grown under low N(LN,0.25 mM)and moderate N(MN,4 mM)conditions.Subsequently,the plants from each N treatment were divided and immediately transferred to ambient(400μmolmol−1)or elevated[CO2](800μmolmol−1).Elevated[CO2]promoted plant growth under both LN andMN conditions through affecting cell division and cell elongation,with a more pronounced effect underMNsupply levels.Elevated[CO2]-induced shoot growth and leaf elongation were associated with increased cytokinin level under LN and with enhanced cytokinin and auxin under MN conditions.Exogenous cytokinin inhibitor(lovastatin)and auxin inhibitor(2,3,5-triiodobenzoic acid)altered elevated[CO2]-enhanced growth in tall fescue regardless of N conditions.Elevation of[CO2]-enhanced growth by modulating cell growth-related genes OsCycD2,OsPCNA,andOsEXPA10was counteracted and reduced in FaCKX11-OE lines under LN and MN conditions,respectively.However,this enhancementwas counteracted in FaDAO-OE lines under MN but not under LN conditions.These results demonstrated that elevated[CO2]-enhanced shoot growth in perennial grass species could be primarily mediated by cytokinin under LN conditions,while both cytokinin and auxin were involved in regulating elevated[CO2]-enhanced growth under MN conditions.展开更多
The mechanical properties of rigid insulation tile(RIT)materials at elevated temperatures(700~1000℃)were studied through compression tests and the digital image correlation(DIC)method.To reduce measurement error in a...The mechanical properties of rigid insulation tile(RIT)materials at elevated temperatures(700~1000℃)were studied through compression tests and the digital image correlation(DIC)method.To reduce measurement error in a thermal environment,an image gradient zero-mean normalized cross-correlation algorithm(ZNCCGI)was added to the DIC algorithm.The DIC algorithm was verified via RIT material mechanical tests at room temperature.Furthermore,the compressive stress–strain curves and Young's modulus of RIT materials at elevated temperatures were obtained.The experimental results show that the Young's modulus of RIT materials significantly increased at 800℃.Moreover,the compressive yield strength was significantly improved at 800℃,which resulted in a random distribution of ceramic fibers and viscous flow deformation at elevated temperatures.Scanning electron microscope analysis demonstrated that the compressive damage occurs due to the breaking of ceramic fibers.展开更多
In tailings permeated by leachate containing thallium(Tl),manganese oxides(MnO x)are recognized as critical substances in immobilizing Tl and preventing its further migration into groundwater.However,dissolved Mn(...In tailings permeated by leachate containing thallium(Tl),manganese oxides(MnO x)are recognized as critical substances in immobilizing Tl and preventing its further migration into groundwater.However,dissolved Mn(Ⅱ)and Fe(Ⅲ)also exist in micro-oxic environments.The effects and mechanisms of the increased levels of these coexisting ions,driven by environmental processes such as rainfall leaching,on Tl(Ⅰ)retention remain largely unclear.This study established two systems using natural manganese sand and limestone sand as porous media,demonstrating that the elevated Mn(Ⅱ)/Fe(Ⅲ)loadings weakened Tl(Ⅰ)retention.Redundancy analysis identified media type and depth as primary factors shaping microbial communities,which subsequently influenced Tl(Ⅰ)immobilization.Manganese sand exhibited superior recovery capacity compared to limestone sand under higher Mn(Ⅱ)and Fe(Ⅲ)loadings.Co-occurrence network analyses revealed that media-microorganism suitability governs microbial community structure and heavy metal retention efficiency.Hydraulic impact and dissolved organic/inorganic cations reduced MnO x content,while the higher retention capacity of manganese sand was attributed to the presence of microorganisms with higher activity and abundance,as well as an increased abundance of microbial-generated MnO_( x).This study offers novel insights into the mechanisms underlying Tl(Ⅰ)retention in tailings,which is crucial for comprehending its environmental fate.展开更多
Thermal charging cells face two main challenges that limit their practical applications.1)Still lacking the systems suitable for operation under higher-temperature environments,even though high-temperature waste-heat ...Thermal charging cells face two main challenges that limit their practical applications.1)Still lacking the systems suitable for operation under higher-temperature environments,even though high-temperature waste-heat recovery systems have greater application potential and practical significance compared with room-temperature systems.2)There are limitations in the self-sustaining performance of continuous discharge under temperature differences,which hold critical significance for the real-world implementation of thermal charging cells.This study has successfully constructed a high-temperature-resistant thermal charging cells system that can operate at 160℃ by optimizing the design of electrode solutions and layered electrode materials,which is currently the highest temperature achieved as far as we know.This high-temperature-resistant thermal charging cells system can achieve a considerable thermal voltage of 960 mV and an impressive Carnot-relative efficiency of 14%,outperforming the state-of-the-art thermoelectric systems.This work has investigated the self-maintained capability of the thermal charging cells system under the opposing effects of ionic concentration and temperature differences between the electrodes and experimentally verified this performance by adjusting the lithium-ion concentration and temperature difference.Furthermore,the stability of the system under long-term charge and discharge cycles was tested,making it the longest running system currently.This work significantly highlighted the broad application prospects of thermal charging cells systems in practical implementations,particularly in advanced thermal energy harvesting and conversion technologies.展开更多
Internal browning(IB)is a common chilling injury(CI)feature in peach fruit after prolonged cold storage.Our previous study demonstrated that low O2and elevated CO2(eCO2)condition of modified atmosphere(MA)sto...Internal browning(IB)is a common chilling injury(CI)feature in peach fruit after prolonged cold storage.Our previous study demonstrated that low O2and elevated CO2(eCO2)condition of modified atmosphere(MA)storage alleviated CI by facilitating the accumulation of jasmonic acids(JAs)andγ-aminobutyric acid(GABA)in‘Zhonghuashoutao’(‘ZHST’)peach fruit.Here we show that 10%CO2alone can improve cold tolerance,with ethylene response factor 17(PpERF17)identified as a pivotal transcription factor(TF)that promotes biosynthesis of JAs and GABA.Stable transformation of PpERF17 in tobacco resulted in reduced cold damage,attributed to decreased levels of hydrogen peroxide(H2O2)and malondialdehyde(MDA),as well as enhanced accumulation of JAs and GABA.Moreover,under eCO2,PpMYC2.1,the master regulator of JA signaling,was found to activate transcription of 13S-lipoxygenase(Pp13S-LOX),allene oxide synthase(PpAOS),12-oxophytodienoate reductase 3(PpOPR3),and glutamate decarboxylase(PpGAD),while also inducing the expression of the upstream TF PpERF17,thereby establishing positive feedback loops upregulating JA and GABA biosynthesis.Finally,application of methyl jasmonate(MeJA)to fruit before shelf transfer from cold storage alleviated chilling injury development,due to increased accumulation of JAs and GABA as a result of raised expression of related biosynthetic genes.Collectively,our results suggest that eCO2-induced PpERF17 enhances JAs and GABA accumulation while activating the JA signaling pathway.This contributes to a positive feedback loop mediated by PpMYC2.1,ultimately alleviating CI of peach fruit through the sustained accumulation of JAs and GABA.展开更多
Soil salinity hampers plant performance.Elevated atmospheric CO2(e[CO2])could alleviate the detrimental effect of salinity on plants but whether abscisic acid(ABA)is involved in this process is unclear.To addres...Soil salinity hampers plant performance.Elevated atmospheric CO2(e[CO2])could alleviate the detrimental effect of salinity on plants but whether abscisic acid(ABA)is involved in this process is unclear.To address this issue,three tomato(Solanum lycopersicum)genotypes with varying endogenous ABA concentrations(wild-type AC,ABA-deficient mutant flacca and ABA-overproduction line SP5)were grown in pots under ambient(400μmol·mol-1)or elevated(800μmol·mol-1)CO2with or without the addition of 100 mmol·L-1sodium chloride(NaCl).The results showed that e[CO2]favored ion homeostasis by decreasing root-to-shoot delivery of Na+,which was mainly attributed to lowered transpiration rate rather than altered xylem-sap Na+concentration.In AC and SP5,the low transpiration rate of e[CO2]-plants under salinity was accompanied by enhanced endogenous ABA levels,which might play a role in upregulating the abundance of specific transcripts related to Na+homeostasis(i.e.,SALT OVERLY SENSITIVE)under salt stress.In flacca,e[CO2]-induced Na+homeostasis was abolished,which could be ascribed to the low and unaltered ABA levels,albeit the ethylene biosynthesis was enhanced in flacca under salt stress,indicating an antagonistic relationship between ABA and ethylene.Furthermore,e[CO2]inhibited ethylene biosynthesis under salt stress in all three genotypes.The results enrich our comprehension of the fundamental processes of e[CO2]-conferred salt tolerance in tomato.展开更多
An experiments were carried out with treatments differing in nitrogen supply (0, 5 and 15 g N/m^2) and CO2 levels (350 and 700 μmol/mol) using OTC (open top chamber) equipment to investigate the biomass of Cala...An experiments were carried out with treatments differing in nitrogen supply (0, 5 and 15 g N/m^2) and CO2 levels (350 and 700 μmol/mol) using OTC (open top chamber) equipment to investigate the biomass of Calamagrostis angustifolia and soil active carbon contents after two years. The results showed that elevated CO2 concentration increased the biomass of C. angustifolia and the magnitude of response varied with each growth period. Elevated CO2 concentration has increased aboveground biomass by 16.7% and 17.6% during the jointing and heading periods and only 3.5% and 9.4% during dough and maturity periods. The increases in belowground biomass due to CO2 elevation was 26.5%, 34.0% and 28.7% during the heading, dough and maturity periods, respectively. The responses of biomass to enhanced CO2 concentrations are differed in N levels. Both the increase of aboveground biomass and belowground biomass were greater under high level of N supply (15 g N/m^2). Elevated CO2 concentration also increased the allocation of biomass and carbon in root. Under elevated CO2 concentration, the average values of active carbon tended to increase. The increases of soil active soil contents followed the sequence of microbial biomass carbon (10.6%) 〉 dissolved organic carbon (7.5%) 〉 labile oxidable carbon (6.6%) 〉 carbohydrate carbon (4.1%). Stepwise regressions indicated there were significant correlations between the soil active carbon contents and plant biomass. Particularly, microbial biomass carbon, labile oxidable carbon and carbohydrate carbon were found to be correlated with belowground biomass, while dissolved organic carbon has correlation with aboveground biomass. Therefore, increased biomass was regarded as the main driving force for the increase in soil active organic carbon under elevated CO2 concentration.展开更多
Despite its negative impacts on plant functioning,climate change benefits plants at the cellular level.For example,the stimulation of C3 photosynthesis by elevated CO2can increase N2 fixation by 73%and grain yield ...Despite its negative impacts on plant functioning,climate change benefits plants at the cellular level.For example,the stimulation of C3 photosynthesis by elevated CO2can increase N2 fixation by 73%and grain yield by 10%–11%.The global elevated atmospheric CO2concentration has already decreased the nitrogen content in C3 crop species and C3 woody vegetation by 14%and 21%,respectively,regardless of added nitrogen fertilizer.15N-feeding experiments have shown that,after 19 h under elevated CO2,the15N concentration in the stems,roots plus rhizomes,and whole plants of Scirpus olneyi(S.olneyi)decreased by 51%,63%,and 74%,respectively.Moreover,S.olneyi showed reduced NH4+assimilation under elevated CO2,which decreased the amino acid contents in the stems by 25.6%for glycine and 65.0%for serine,and that in the roots plus rhizomes by 2%for gamma-aminobutyric acid(GABA)and 80%for glutamate.Wheat grain protein has also been found to decrease by 7.4%under elevated CO2due to reductions in threonine,valine,iso-leucine,leucine,and phenylalanine.The mineral nutrient contents in grains of rice and maize were similarly found to decrease under high CO2by 1.0%and 7.1%for phosphorus,7.8%and 2.1%for sulfur,5.2%and 5.8%for iron,3.3%and 5.2%for zinc,10.6%and 9.9%for copper,and 7.5%and 4.2%for manganese,respectively.In general,mineral concentrations in C3 plants are predicted to decrease by 8%under elevated CO2,while total non-structural carbohydrates(mainly starch and sugars)are expected to increase.These decreases in grain protein,amino acids,and mineral nutrients could double the incidence of global protein-calorie malnutrition and micronutrient deficiency—especially in Africa,where agricultural soils are inherently low in nutrient elements.Additionally,the increase in total non-structural carbohydrates(mainly starch and sugars)in cereal crops could elevate diabetes incidence due to heavy reliance on starchy diets.The negative effects of elevated CO2on rice,maize,and wheat—the world's three major staple crops—suggest an increase in global food insecurity with rising atmospheric CO2concentration.展开更多
COcapture with ionic liquids(ILs) has attracted many attentions, and most works focused on absorption ability at ambient temperatures, while seldom research was concerned at elevated temperatures.This not only limit...COcapture with ionic liquids(ILs) has attracted many attentions, and most works focused on absorption ability at ambient temperatures, while seldom research was concerned at elevated temperatures.This not only limits the COabsorption application at elevated temperature, but also the determination of the operation condition of the COdesorption generally occurring at higher temperature. This work mainly reported COsolubilities in ILs at elevated temperatures and related properties were also provided. 1-alkyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide([CnMIm][TfN]) ILs were selected as physical absorbents for COcapture in this work due to their relative higher COabsorption capacities and good thermal stabilities. The long-term stability tests showed that [CnMIm][TfN] is thermally stable at 393.15 K for long time. COsolubilities in [CnMIm][TfN] were systematically determined at temperatures from 353.15 K to 393.15 K. It demonstrated that COsolubility obviously increases with the increase of pressure while slightly decreases with increase of temperature. As the length of alkyl chain on the cation increases, COsolubility in ILs increases. Additionally, the thermodynamic properties including the Gibbs free energy, enthalpy, and entropy of COwere also calculated.展开更多
Cyanobacterial blooms,exacerbated by climate change and eutrophication,increasingly compromise water quality by producing taste and odor(T&O)compounds including 2-methylisoborneol(MIB).Cyanobacteria synthesize MIB...Cyanobacterial blooms,exacerbated by climate change and eutrophication,increasingly compromise water quality by producing taste and odor(T&O)compounds including 2-methylisoborneol(MIB).Cyanobacteria synthesize MIB by redirecting carbon metabolism intermediates into the methylerythritol phosphate pathway.This process appears to be influenced by rising atmospheric CO2 partial pressure(pCO2).This study examines how elevated pCO2 affects MIB production in Synechococcus sp.FACHB-1061 under varying nutrient conditions.The results indicate that elevated pCO2 boosts cyanobacterial biomass and total MIB yield,particularly in nutrient-rich environments,by reducing limitations in bioavailable inorganic carbon.In contrast,in nutrient-limited conditions,both total MIB concentration and per-cell production significantly increased with higher pCO2.Elevated pCO2 altered the activities of nitrate reductase and carbonic anhydrase,reducing impaired nitrogen assimilation and enhanced carbon fixation.The increase and compositional changes in extracellular polymeric substances suggested carbon overflow and a decline in cellular quality under insufficient nutrients.Additionally,markers of oxidative stress,such as increased superoxide dismutase activity and higher malondialdehyde levels,suggested that oxidative stress might stimulate secondary metabolite production.Metabolomic analysis revealed that critical intermediates of the Calvin cycle and glycolysis,such as glyceraldehyde-3-phosphate and acetyl-CoA,were upregulated with elevated pCO2 in nutrient-limited status,leading to increase of key precursors for MIB.These results highlight the complex interplay between nutrient and rising pCO2 in regulating cyanobacterial MIB biosynthesis.The findings suggest that conventional nutrient control strategies for mitigating cyanobacterial blooms and associated T&O issues may need reassessment under future climate scenarios.展开更多
Effects of elevated CO, (5000 μl/L) on sensitivity comparison of six species of algae and interspecific competition of three species of algae were investigated. The results showed that, the cell densities of six sp...Effects of elevated CO, (5000 μl/L) on sensitivity comparison of six species of algae and interspecific competition of three species of algae were investigated. The results showed that, the cell densities of six species of algae grown in elevated CO2 significantly increased compared to those in ambient CO2 (360 μl/L), and with the time prolonged, the increasing extent increased. Therefore, elevated CO2 can promote the growth of six species of algae. However, there were differences in sensitivity between six species of algae. Based on the effects of elevated CO2 on biomass, the sensitive order (from high to low) was Platymanas sp., Platymanas subcordiformis, Nitzschia closterium, Isochrysis golbana Parke 8701, Dunoliella salina, Chlorella sp., on the condition of solitary cultivation. Compared to ambient CO2, elevated CO2 promoted the growth of three species of algae, Platymanas subcordiformis, Nitzschia closterium and Isochrysis galbana Parke 8701 under the condition of mixed cultivation. The sensitivity of the three species to elevated CO2 in mixed cultivation changed a lot compared to the condition of solitary cultivation. When grown in elevated CO2 under the condition of mixed cultivation, the sensitive order from high to low were Nitzschia clostertium, Platymonas subcordiformis; and Isochrysis galbana Parke 8701. However, under the condition of solitary cultivation, the sensitive order in elevated CO2 was Isochrysis galbana Parke 8701, Nitzschia clostertium, Platymonas subcordiformis, from sensitive to less sensitive. On the day 21, the dominant algae, the sub-dominant algae and inferior algae grown in elevated CO2 did not change. However, the population increasing dynamic and composition proportion of three algal species have significantly changed.展开更多
The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera (Htibner), fed on cotton bolls grown under elevated CO2 (double-ambient vs. ambient) in open-top cha...The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera (Htibner), fed on cotton bolls grown under elevated CO2 (double-ambient vs. ambient) in open-top chambers were examined. Significant decreases in protein, total amino acid, water and nitrogen content and increases in free fatty acid were observed in cotton bolls. Changes in quality of cotton bolls affected the growth, development and food utilization of H. armigera. Significantly longer larval development duration in three successive generations and lower pupal weight of the second and third generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower fecundity was also found in successive three generations of H. armigera fed on cotton bolls grown under elevated CO2. The consumption per larva occurred significant increase in successive three generations and frass per larva were also significantly increased during the second and third generations under elevated CO2. Significantly lower relative growth rate, efficiency of conversion of ingested food and significant higher relative consumption rate in successive three generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower potential female fecundity, larval numbers and population consumption were found in the second and third generations of cotton bollworm fed on cotton bolls grown under elevated CO2. The integrative effect of higher larval mortality rate and lower adult fecundity resulted in significant decreases in potential population consumption in the latter two generations. The results show that elevated CO2 adversely affects cotton bolls quality, which indicates the potential population dynamics and potential population consumption of cotton bollworm will alleviate the harm to the plants in the future rising CO2 atmosphere.展开更多
This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two C...This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0,4,and 16 mg/L) under hydroponic conditions.The results show that elevated levels of CO2 increased shoot biomass more,compared to root biomass,but decreased Cd concentrations in all plant tissues.Cd exposure caused toxicity to both Lolium species,as shown by the restrictions of the root morphological parameters including root length,surface area,volume,and tip numbers.These parameters were significantly higher under elevated levels of CO2 than under ambient CO2,especially for the number of fine roots.The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress,suggesting an ameliorated Cd stress under elevated levels of CO2.The total Cd uptake per pot,calculated on the basis of biomass,was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity,decreased Cd concentration,and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.展开更多
In this work,we coated a layer of Y2O3 particles in Hastelloy X(HX)nickel-based superalloy powder by in situ chemical method and combined with laser powder bed fusion(LPBF)technology to develop a high-performanc...In this work,we coated a layer of Y2O3 particles in Hastelloy X(HX)nickel-based superalloy powder by in situ chemical method and combined with laser powder bed fusion(LPBF)technology to develop a high-performance Y2O3-doping alloy,designated as Y-HX.The results show that the doping of Y2O3 particles prevents crack formation during the printing process and reduces solute segregation at cell and grain boundaries by increasing the viscosity of the molten pool.The doping of Y2O3 particles to the printed Y-HX alloy enhances grain boundary characteristics,transforming coarse sheet-like carbides into finely dispersed granular carbides at the boundaries during subsequent heat treatment.Additionally,doping with Y2O3 particles increases the recrystallization activation energy of the Y-HX alloy from 149.4 to 278.8 kJ mol-1.At 750℃,the Y-HX alloy exhibits an ultimate tensile strength of 619±2 MPa and an elongation of 52%±2%,along with an ultimate tensile strength of 325±3 MPa and an elongation of 47%±2% at 900℃.Our work provides a promising way to develop additive-manufactured superalloys with exceptional thermal stability and remarkable high-temperature mechanical properties.展开更多
A study was conducted to determine the effects of elevated CO2 on soil N process at Changbai Mountain in Jilin Province, northeastern China (42°24"N, 128°06"E, and 738 m elevation). A randomized complete...A study was conducted to determine the effects of elevated CO2 on soil N process at Changbai Mountain in Jilin Province, northeastern China (42°24"N, 128°06"E, and 738 m elevation). A randomized complete block design of ambient and elevated CO2 was established in an open-top chamber facility in the spring of 1999. Changpai Scotch pine (Pinus sylvestris var. sylvestriformis seeds were sowed in May, 1999 and CO2 fumigation treatments began after seeds germination. In each year, the exposure started at the end of April and stopped at the end of October. Soil samples were collected in June and August 2006 and in June 2007, and soil nitrifying, denitrifying and N2-fixing enzyme activities were measured. Results show that soil nitrifying enzyme activities (NEA) in the 5-10 cm soil layer were significantly increased at elevated CO2 by 30.3% in June 2006, by 30.9% in August 2006 and by 11.3% in June 2007. Soil denitrifying enzyme activities (DEA) were significantly decreased by elevated CO2 treatment in June 2006 (P 〈 0.012) and August 2006 (P 〈 0.005) samplings in our study; no significant difference was detected in June 2007, and no significant changes in N2-fixing enzyme activity were found. This study suggests that elevated CO2 can alter soil nitrifying enzyme and denitrifying enzyme activities.展开更多
Global environmental change affects plant physiological and ecosystem processes. The interaction of elevated CO2, drought and nitrogen (N) deficiency result in complex responses of C4 species photosynthetic process ...Global environmental change affects plant physiological and ecosystem processes. The interaction of elevated CO2, drought and nitrogen (N) deficiency result in complex responses of C4 species photosynthetic process that challenge our current understanding. An experiment of maize (Zea mays L.) involving CO2 concentrations (380 or 750 μmol mol1, climate chamber), osmotic stresses (10% PEG-6000, -0.32 MPa) and nitrogen constraints (N deficiency treated since the 144th drought hour) was carried out to investigate its photosynthesis capacity and leaf nitrogen use efficiency. Elevated CO2 could alleviate drought-induced photosynthetic limitation through increasing capacity of PEPC carboxylation (Vp~,x) and decreasing stomatal limitations (SL). The N deficiency exacerbated drought-induced photosynthesis limitations in ambient CO2. Elevated CO2 partially alleviated the limitation induced by drought and N deficiency through improving the capacity of Rubisco carboxylation (Vmax) and decreasing SL. Plants with N deficiency transported more N to their leaves at elevated CO2, leading to a high photosynthetic nitrogen-use efficiency but low whole-plant nitrogen-use efficiency. The stress mitigation by elevated CO2 under N deficiency conditions was not enough to improving plant N use efficiency and biomass accumulation. The study demonstrated that elevated CO2 could alleviate drought-induced photosynthesis limitation, but the alleviation varied with N supplies.展开更多
The objectives of this study were to investigate the effect of higher CO2 concentrations (500 and 700 μmol mol^-1) in atmosphere on total soil respiration and the contribution of root respiration to total soil resp...The objectives of this study were to investigate the effect of higher CO2 concentrations (500 and 700 μmol mol^-1) in atmosphere on total soil respiration and the contribution of root respiration to total soil respiration during seedling growth of Pinus sylvestris vat. sylvestriformis. During the four growing seasons (May-October) from 1999 to 2003, the seedlings were exposed to elevated concentrations of CO2 in open-top chambers. The total soil respiration and contribution of root respiration were measured using an LI-6400-09 soil CO2 flux chamber on June 15 and October 8, 2003. To separate root respiration from total soil respiration, three PVC cylinders were inserted approximately 30 cm deep into the soil in each chamber. There were marked diurnal changes in air and soil temperatures on June 15. Both the total soil respiration and the soil respiration without roots showed a strong diurnal pattern, increasing from before sunrise to about 14:00 in the afternoon and then decreasing before the next sunrise. No increase in the mean total soil respiration and mean soil respiration with roots severed was observed under the elevated CO2 treatments on June 15, 2003, as compared to the open field and control chamber with ambient CO2. However, on October 8, 2003, the total soil respiration and soil respiration with roots severed in the open field were lower than those in the control and elevated CO2 chambers. The mean contribution of root respiration measured on June 15, 2003, ranged from 8.3% to 30.5% and on October 8, 2003, from 20.6% to 48.6%.展开更多
Exotic plant invasions and increased atmospheric carbon dioxide(CO2)concentration have been determined to independently affect soil nematodes,a key component of soil biota.However,little is known about the long-ter...Exotic plant invasions and increased atmospheric carbon dioxide(CO2)concentration have been determined to independently affect soil nematodes,a key component of soil biota.However,little is known about the long-term effects of these two global change factors and their interactive effects.Over three consecutive years,we cultivated invasive alien plant Xanthium strumarium and its two phylogenetically related natives under both ambient(aCO2)and elevated(eCO2)atmospheric CO2concentrations,and determined the effects of the invader and natives on soil nematodes under different CO2concentrations and the relevant mechanism.The abundance of total soil nematodes and that of the dominant trophic group(herbivores)were significantly affected by plant species and CO2concentration,and these effects were dependent on the experimental duration,however,the Shannon-diversity of nematodes was not affected by these factors.Under aCO2,both invasive and native species significantly increased the total nematode abundance and that of the dominant trophic group with increasing experimental duration,and the amplitude of the increase was greater under the invader relative to the natives.The eCO2increased total nematode abundance(second year)and that of the dominant trophic group(third year)under the invader,but not under the natives(or even decreased)with increasing experimental duration.Root litter had greater effects on soil nematode abundance than leaf litter and root exudates did.This study indicates that eCO2would aggravate effects of invasive plants on soil nematodes by increasing abundance,and these effects would vary with the duration.展开更多
The maintenance of rapid growth under conditions of CO2 enrichment is directly related to the capacity of new leaves to use or store the additional assimilated carbon (C) and nitrogen (N). Under drought conditions...The maintenance of rapid growth under conditions of CO2 enrichment is directly related to the capacity of new leaves to use or store the additional assimilated carbon (C) and nitrogen (N). Under drought conditions, however, less is known about C and N transport in C4 plants and the contributions of these processes to new foliar growth. We measured the patterns of C and N accumulation in maize (Zea mays L.) seedlings using 13C and 15N as tracers in CO2 climate chambers (380 or 750 μmol mol-1) under a mild drought stress induced with 10% PEG-6000. The drought stress under ambient conditions decreased the biomass production of the maize plants; however, this effect was reduced under elevated CO2. Compared with the water-stressed maize plants under atmospheric CO2, the treatment that combined elevated CO2 with water stress increased the accumulation of biomass, partitioned more C and N to new leaves as well as enhanced the carbon resource in ageing leaves and the carbon pool in new leaves. However, the C counterflow capability of the roots decreased. The elevated CO2 increased the time needed for newly acquired N to be present in the roots and increased the proportion of new N in the leaves. The maize plants supported the development of new leaves at elevated CO2 by altering the transport and remobilization of C and N. Under drought conditions, the increased activity of new leaves in relation to the storage of C and N sustained the enhanced growth of these plants under elevated CO2.展开更多
Elevated,rectangular water tanks are an essential component of the water supply network and for emergency water storage.However,determining the dynamic response from seismic loads will require accurate estimates of th...Elevated,rectangular water tanks are an essential component of the water supply network and for emergency water storage.However,determining the dynamic response from seismic loads will require accurate estimates of their natural frequencies to help avoid damage or failure.Several numerical and analytical methodologies depend on assumptions that may not account for the complexity of fluid-structure interaction regarding rectangular geometries,indicating a strong need for solid experimental validation.This study pioneers the application of Digital Image Correlation(DIC),a non-contact optical technique,to measure the natural frequencies of a small-scale acrylic and Teflon tank,addressing this gap with a novel experimental approach.The method includes DIC using a high-speed camera,image analyzed by MATLAB,frequency analysis by Continuous Wavelet Transformation(CWT),and Fast Fourier Transformation(FFT);ANSYS finite element analysis,simplified models based on Eurocode and the Egyptian Code of Practice(ECP).Study results indicate that DIC is critical for attaining high accuracy,with maximum error differing by 2.92% for impulsive and 4.55%for convective frequencies from ANSYS and provides a better measure of dynamic response compared to contact-based measurements.Impulsive frequency decreased from 5.8724 Hz to 4.0085 Hz,and sloshing increased from 1.00 Hz to 1.84 Hz,as the water cover height varied from 0 cm to 9.8 cm.The Eurocode and ECP models describe acceptable errors of 7.36% and 9.21%,respectively.DIC showed higher accuracy,making it a useful tool for seismic design.This study improves the safety and reliability of designs for elevated water tanks in seismic regions with elevated seismic risk.展开更多
基金supported by the National Natural Science Foundation of China(32471773,32401474).
摘要Elevated atmospheric[CO2]and nitrogen(N)availability are critical determinants of plant growth.This study investigated the underlying mechanisms of hormones in mediating elevated[CO2]-promoted shoot growth and leaf elongation under different N conditions in tall fescue(Festuca arundinacea).Plants were grown under low N(LN,0.25 mM)and moderate N(MN,4 mM)conditions.Subsequently,the plants from each N treatment were divided and immediately transferred to ambient(400μmolmol−1)or elevated[CO2](800μmolmol−1).Elevated[CO2]promoted plant growth under both LN andMN conditions through affecting cell division and cell elongation,with a more pronounced effect underMNsupply levels.Elevated[CO2]-induced shoot growth and leaf elongation were associated with increased cytokinin level under LN and with enhanced cytokinin and auxin under MN conditions.Exogenous cytokinin inhibitor(lovastatin)and auxin inhibitor(2,3,5-triiodobenzoic acid)altered elevated[CO2]-enhanced growth in tall fescue regardless of N conditions.Elevation of[CO2]-enhanced growth by modulating cell growth-related genes OsCycD2,OsPCNA,andOsEXPA10was counteracted and reduced in FaCKX11-OE lines under LN and MN conditions,respectively.However,this enhancementwas counteracted in FaDAO-OE lines under MN but not under LN conditions.These results demonstrated that elevated[CO2]-enhanced shoot growth in perennial grass species could be primarily mediated by cytokinin under LN conditions,while both cytokinin and auxin were involved in regulating elevated[CO2]-enhanced growth under MN conditions.
基金The National Natural Science Foundation of China(Grant Nos.12472210 and 11902046)the Natural Science Basic Research Plan in Shaanxi Province of China(Grant No.2023-JC-YB-031)the China Postdoctoral Science Foundation(Grant Nos.2021T140635 and 2020M673580XB)contributed financially to this study.
摘要The mechanical properties of rigid insulation tile(RIT)materials at elevated temperatures(700~1000℃)were studied through compression tests and the digital image correlation(DIC)method.To reduce measurement error in a thermal environment,an image gradient zero-mean normalized cross-correlation algorithm(ZNCCGI)was added to the DIC algorithm.The DIC algorithm was verified via RIT material mechanical tests at room temperature.Furthermore,the compressive stress–strain curves and Young's modulus of RIT materials at elevated temperatures were obtained.The experimental results show that the Young's modulus of RIT materials significantly increased at 800℃.Moreover,the compressive yield strength was significantly improved at 800℃,which resulted in a random distribution of ceramic fibers and viscous flow deformation at elevated temperatures.Scanning electron microscope analysis demonstrated that the compressive damage occurs due to the breaking of ceramic fibers.
基金supported by the National Natural Science Foundation of China(No.52422005)the Natural Science Foundation of Chongqing,China(No.CSTB2025NSCQ-JQX0029).
摘要In tailings permeated by leachate containing thallium(Tl),manganese oxides(MnO x)are recognized as critical substances in immobilizing Tl and preventing its further migration into groundwater.However,dissolved Mn(Ⅱ)and Fe(Ⅲ)also exist in micro-oxic environments.The effects and mechanisms of the increased levels of these coexisting ions,driven by environmental processes such as rainfall leaching,on Tl(Ⅰ)retention remain largely unclear.This study established two systems using natural manganese sand and limestone sand as porous media,demonstrating that the elevated Mn(Ⅱ)/Fe(Ⅲ)loadings weakened Tl(Ⅰ)retention.Redundancy analysis identified media type and depth as primary factors shaping microbial communities,which subsequently influenced Tl(Ⅰ)immobilization.Manganese sand exhibited superior recovery capacity compared to limestone sand under higher Mn(Ⅱ)and Fe(Ⅲ)loadings.Co-occurrence network analyses revealed that media-microorganism suitability governs microbial community structure and heavy metal retention efficiency.Hydraulic impact and dissolved organic/inorganic cations reduced MnO x content,while the higher retention capacity of manganese sand was attributed to the presence of microorganisms with higher activity and abundance,as well as an increased abundance of microbial-generated MnO_( x).This study offers novel insights into the mechanisms underlying Tl(Ⅰ)retention in tailings,which is crucial for comprehending its environmental fate.
基金financially supported by the Basic Science Center Program for Ordered Energy Conversion of the National Natural Science Foundation of China(no.52488201)the Natural Science Foundation of Jiangsu Province(no.BK20202008).
摘要Thermal charging cells face two main challenges that limit their practical applications.1)Still lacking the systems suitable for operation under higher-temperature environments,even though high-temperature waste-heat recovery systems have greater application potential and practical significance compared with room-temperature systems.2)There are limitations in the self-sustaining performance of continuous discharge under temperature differences,which hold critical significance for the real-world implementation of thermal charging cells.This study has successfully constructed a high-temperature-resistant thermal charging cells system that can operate at 160℃ by optimizing the design of electrode solutions and layered electrode materials,which is currently the highest temperature achieved as far as we know.This high-temperature-resistant thermal charging cells system can achieve a considerable thermal voltage of 960 mV and an impressive Carnot-relative efficiency of 14%,outperforming the state-of-the-art thermoelectric systems.This work has investigated the self-maintained capability of the thermal charging cells system under the opposing effects of ionic concentration and temperature differences between the electrodes and experimentally verified this performance by adjusting the lithium-ion concentration and temperature difference.Furthermore,the stability of the system under long-term charge and discharge cycles was tested,making it the longest running system currently.This work significantly highlighted the broad application prospects of thermal charging cells systems in practical implementations,particularly in advanced thermal energy harvesting and conversion technologies.
基金supported by the National Key Research and Development Program of China(2022YFD2100103)Ningbo Key Research and Development Program(2022Z179)+1 种基金Zhejiang Provincial Cooperative Extension Project of Agricultural Key Technology(2022XTTGGP01)the 111 Project(B17039).
摘要Internal browning(IB)is a common chilling injury(CI)feature in peach fruit after prolonged cold storage.Our previous study demonstrated that low O2and elevated CO2(eCO2)condition of modified atmosphere(MA)storage alleviated CI by facilitating the accumulation of jasmonic acids(JAs)andγ-aminobutyric acid(GABA)in‘Zhonghuashoutao’(‘ZHST’)peach fruit.Here we show that 10%CO2alone can improve cold tolerance,with ethylene response factor 17(PpERF17)identified as a pivotal transcription factor(TF)that promotes biosynthesis of JAs and GABA.Stable transformation of PpERF17 in tobacco resulted in reduced cold damage,attributed to decreased levels of hydrogen peroxide(H2O2)and malondialdehyde(MDA),as well as enhanced accumulation of JAs and GABA.Moreover,under eCO2,PpMYC2.1,the master regulator of JA signaling,was found to activate transcription of 13S-lipoxygenase(Pp13S-LOX),allene oxide synthase(PpAOS),12-oxophytodienoate reductase 3(PpOPR3),and glutamate decarboxylase(PpGAD),while also inducing the expression of the upstream TF PpERF17,thereby establishing positive feedback loops upregulating JA and GABA biosynthesis.Finally,application of methyl jasmonate(MeJA)to fruit before shelf transfer from cold storage alleviated chilling injury development,due to increased accumulation of JAs and GABA as a result of raised expression of related biosynthetic genes.Collectively,our results suggest that eCO2-induced PpERF17 enhances JAs and GABA accumulation while activating the JA signaling pathway.This contributes to a positive feedback loop mediated by PpMYC2.1,ultimately alleviating CI of peach fruit through the sustained accumulation of JAs and GABA.
基金supported by the Chinese Scholarship Council(CSC).
摘要Soil salinity hampers plant performance.Elevated atmospheric CO2(e[CO2])could alleviate the detrimental effect of salinity on plants but whether abscisic acid(ABA)is involved in this process is unclear.To address this issue,three tomato(Solanum lycopersicum)genotypes with varying endogenous ABA concentrations(wild-type AC,ABA-deficient mutant flacca and ABA-overproduction line SP5)were grown in pots under ambient(400μmol·mol-1)or elevated(800μmol·mol-1)CO2with or without the addition of 100 mmol·L-1sodium chloride(NaCl).The results showed that e[CO2]favored ion homeostasis by decreasing root-to-shoot delivery of Na+,which was mainly attributed to lowered transpiration rate rather than altered xylem-sap Na+concentration.In AC and SP5,the low transpiration rate of e[CO2]-plants under salinity was accompanied by enhanced endogenous ABA levels,which might play a role in upregulating the abundance of specific transcripts related to Na+homeostasis(i.e.,SALT OVERLY SENSITIVE)under salt stress.In flacca,e[CO2]-induced Na+homeostasis was abolished,which could be ascribed to the low and unaltered ABA levels,albeit the ethylene biosynthesis was enhanced in flacca under salt stress,indicating an antagonistic relationship between ABA and ethylene.Furthermore,e[CO2]inhibited ethylene biosynthesis under salt stress in all three genotypes.The results enrich our comprehension of the fundamental processes of e[CO2]-conferred salt tolerance in tomato.
基金supported by the Chinese Academy of Sciences (No KZCX2-YW-309)the National Basic Research Program (973) of China (No 2004CB418507)
摘要An experiments were carried out with treatments differing in nitrogen supply (0, 5 and 15 g N/m^2) and CO2 levels (350 and 700 μmol/mol) using OTC (open top chamber) equipment to investigate the biomass of Calamagrostis angustifolia and soil active carbon contents after two years. The results showed that elevated CO2 concentration increased the biomass of C. angustifolia and the magnitude of response varied with each growth period. Elevated CO2 concentration has increased aboveground biomass by 16.7% and 17.6% during the jointing and heading periods and only 3.5% and 9.4% during dough and maturity periods. The increases in belowground biomass due to CO2 elevation was 26.5%, 34.0% and 28.7% during the heading, dough and maturity periods, respectively. The responses of biomass to enhanced CO2 concentrations are differed in N levels. Both the increase of aboveground biomass and belowground biomass were greater under high level of N supply (15 g N/m^2). Elevated CO2 concentration also increased the allocation of biomass and carbon in root. Under elevated CO2 concentration, the average values of active carbon tended to increase. The increases of soil active soil contents followed the sequence of microbial biomass carbon (10.6%) 〉 dissolved organic carbon (7.5%) 〉 labile oxidable carbon (6.6%) 〉 carbohydrate carbon (4.1%). Stepwise regressions indicated there were significant correlations between the soil active carbon contents and plant biomass. Particularly, microbial biomass carbon, labile oxidable carbon and carbohydrate carbon were found to be correlated with belowground biomass, while dissolved organic carbon has correlation with aboveground biomass. Therefore, increased biomass was regarded as the main driving force for the increase in soil active organic carbon under elevated CO2 concentration.
基金supported by the Nanfan special project,CAAS(YBXM2408)the Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSIAF-202303)to Huihui Li+1 种基金a grant from Sanya Municipal Program for Science and Technology Innovation(2022KJCX87)the Nanfan special project,CAAS(YBXM2319),to Jun Zhao。
摘要Despite its negative impacts on plant functioning,climate change benefits plants at the cellular level.For example,the stimulation of C3 photosynthesis by elevated CO2can increase N2 fixation by 73%and grain yield by 10%–11%.The global elevated atmospheric CO2concentration has already decreased the nitrogen content in C3 crop species and C3 woody vegetation by 14%and 21%,respectively,regardless of added nitrogen fertilizer.15N-feeding experiments have shown that,after 19 h under elevated CO2,the15N concentration in the stems,roots plus rhizomes,and whole plants of Scirpus olneyi(S.olneyi)decreased by 51%,63%,and 74%,respectively.Moreover,S.olneyi showed reduced NH4+assimilation under elevated CO2,which decreased the amino acid contents in the stems by 25.6%for glycine and 65.0%for serine,and that in the roots plus rhizomes by 2%for gamma-aminobutyric acid(GABA)and 80%for glutamate.Wheat grain protein has also been found to decrease by 7.4%under elevated CO2due to reductions in threonine,valine,iso-leucine,leucine,and phenylalanine.The mineral nutrient contents in grains of rice and maize were similarly found to decrease under high CO2by 1.0%and 7.1%for phosphorus,7.8%and 2.1%for sulfur,5.2%and 5.8%for iron,3.3%and 5.2%for zinc,10.6%and 9.9%for copper,and 7.5%and 4.2%for manganese,respectively.In general,mineral concentrations in C3 plants are predicted to decrease by 8%under elevated CO2,while total non-structural carbohydrates(mainly starch and sugars)are expected to increase.These decreases in grain protein,amino acids,and mineral nutrients could double the incidence of global protein-calorie malnutrition and micronutrient deficiency—especially in Africa,where agricultural soils are inherently low in nutrient elements.Additionally,the increase in total non-structural carbohydrates(mainly starch and sugars)in cereal crops could elevate diabetes incidence due to heavy reliance on starchy diets.The negative effects of elevated CO2on rice,maize,and wheat—the world's three major staple crops—suggest an increase in global food insecurity with rising atmospheric CO2concentration.
基金supported by the National Natural Science Foundation of China (21606233, 21436010)the National Natural Science Fund for Distinguished Young Scholars (21425625)the Research Council of Norway through the CLIMIT program (215732)
摘要COcapture with ionic liquids(ILs) has attracted many attentions, and most works focused on absorption ability at ambient temperatures, while seldom research was concerned at elevated temperatures.This not only limits the COabsorption application at elevated temperature, but also the determination of the operation condition of the COdesorption generally occurring at higher temperature. This work mainly reported COsolubilities in ILs at elevated temperatures and related properties were also provided. 1-alkyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide([CnMIm][TfN]) ILs were selected as physical absorbents for COcapture in this work due to their relative higher COabsorption capacities and good thermal stabilities. The long-term stability tests showed that [CnMIm][TfN] is thermally stable at 393.15 K for long time. COsolubilities in [CnMIm][TfN] were systematically determined at temperatures from 353.15 K to 393.15 K. It demonstrated that COsolubility obviously increases with the increase of pressure while slightly decreases with increase of temperature. As the length of alkyl chain on the cation increases, COsolubility in ILs increases. Additionally, the thermodynamic properties including the Gibbs free energy, enthalpy, and entropy of COwere also calculated.
基金supported by the National Natural Science Foundation of China(No.41977387)the National Research Foundation,Prime Minister’s Office,Singapore under its Campus for Research Excellence and Technological Enterprise(CREATE)program.
摘要Cyanobacterial blooms,exacerbated by climate change and eutrophication,increasingly compromise water quality by producing taste and odor(T&O)compounds including 2-methylisoborneol(MIB).Cyanobacteria synthesize MIB by redirecting carbon metabolism intermediates into the methylerythritol phosphate pathway.This process appears to be influenced by rising atmospheric CO2 partial pressure(pCO2).This study examines how elevated pCO2 affects MIB production in Synechococcus sp.FACHB-1061 under varying nutrient conditions.The results indicate that elevated pCO2 boosts cyanobacterial biomass and total MIB yield,particularly in nutrient-rich environments,by reducing limitations in bioavailable inorganic carbon.In contrast,in nutrient-limited conditions,both total MIB concentration and per-cell production significantly increased with higher pCO2.Elevated pCO2 altered the activities of nitrate reductase and carbonic anhydrase,reducing impaired nitrogen assimilation and enhanced carbon fixation.The increase and compositional changes in extracellular polymeric substances suggested carbon overflow and a decline in cellular quality under insufficient nutrients.Additionally,markers of oxidative stress,such as increased superoxide dismutase activity and higher malondialdehyde levels,suggested that oxidative stress might stimulate secondary metabolite production.Metabolomic analysis revealed that critical intermediates of the Calvin cycle and glycolysis,such as glyceraldehyde-3-phosphate and acetyl-CoA,were upregulated with elevated pCO2 in nutrient-limited status,leading to increase of key precursors for MIB.These results highlight the complex interplay between nutrient and rising pCO2 in regulating cyanobacterial MIB biosynthesis.The findings suggest that conventional nutrient control strategies for mitigating cyanobacterial blooms and associated T&O issues may need reassessment under future climate scenarios.
摘要Effects of elevated CO, (5000 μl/L) on sensitivity comparison of six species of algae and interspecific competition of three species of algae were investigated. The results showed that, the cell densities of six species of algae grown in elevated CO2 significantly increased compared to those in ambient CO2 (360 μl/L), and with the time prolonged, the increasing extent increased. Therefore, elevated CO2 can promote the growth of six species of algae. However, there were differences in sensitivity between six species of algae. Based on the effects of elevated CO2 on biomass, the sensitive order (from high to low) was Platymanas sp., Platymanas subcordiformis, Nitzschia closterium, Isochrysis golbana Parke 8701, Dunoliella salina, Chlorella sp., on the condition of solitary cultivation. Compared to ambient CO2, elevated CO2 promoted the growth of three species of algae, Platymanas subcordiformis, Nitzschia closterium and Isochrysis galbana Parke 8701 under the condition of mixed cultivation. The sensitivity of the three species to elevated CO2 in mixed cultivation changed a lot compared to the condition of solitary cultivation. When grown in elevated CO2 under the condition of mixed cultivation, the sensitive order from high to low were Nitzschia clostertium, Platymonas subcordiformis; and Isochrysis galbana Parke 8701. However, under the condition of solitary cultivation, the sensitive order in elevated CO2 was Isochrysis galbana Parke 8701, Nitzschia clostertium, Platymonas subcordiformis, from sensitive to less sensitive. On the day 21, the dominant algae, the sub-dominant algae and inferior algae grown in elevated CO2 did not change. However, the population increasing dynamic and composition proportion of three algal species have significantly changed.
基金Project supported by the National Basic Research Program(973)of China(No.2006CB102002)the Pilot Project of Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-YW-N-006)National Natural Science Foundation of China(No.30571253,30621003)
摘要The growth, development and consumption of successive three generations of cotton bollworm, Helicoverpa armigera (Htibner), fed on cotton bolls grown under elevated CO2 (double-ambient vs. ambient) in open-top chambers were examined. Significant decreases in protein, total amino acid, water and nitrogen content and increases in free fatty acid were observed in cotton bolls. Changes in quality of cotton bolls affected the growth, development and food utilization of H. armigera. Significantly longer larval development duration in three successive generations and lower pupal weight of the second and third generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower fecundity was also found in successive three generations of H. armigera fed on cotton bolls grown under elevated CO2. The consumption per larva occurred significant increase in successive three generations and frass per larva were also significantly increased during the second and third generations under elevated CO2. Significantly lower relative growth rate, efficiency of conversion of ingested food and significant higher relative consumption rate in successive three generations were observed in cotton bollworm fed on cotton bolls grown under elevated CO2. Significantly lower potential female fecundity, larval numbers and population consumption were found in the second and third generations of cotton bollworm fed on cotton bolls grown under elevated CO2. The integrative effect of higher larval mortality rate and lower adult fecundity resulted in significant decreases in potential population consumption in the latter two generations. The results show that elevated CO2 adversely affects cotton bolls quality, which indicates the potential population dynamics and potential population consumption of cotton bollworm will alleviate the harm to the plants in the future rising CO2 atmosphere.
基金Project supported by the Central Public Research Institute Basic Fund for Research and Development (2008-jxh-1),Agro-environmental Protection Institute,Ministry of Agriculture,China
摘要This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0,4,and 16 mg/L) under hydroponic conditions.The results show that elevated levels of CO2 increased shoot biomass more,compared to root biomass,but decreased Cd concentrations in all plant tissues.Cd exposure caused toxicity to both Lolium species,as shown by the restrictions of the root morphological parameters including root length,surface area,volume,and tip numbers.These parameters were significantly higher under elevated levels of CO2 than under ambient CO2,especially for the number of fine roots.The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress,suggesting an ameliorated Cd stress under elevated levels of CO2.The total Cd uptake per pot,calculated on the basis of biomass,was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity,decreased Cd concentration,and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.
基金the National Key R&D Program of China(No.2023YFB3712002)the National Natural Science Founda-tion of China(Grant Nos.U22A20172 and 52171044)the Seed Foundation of Tianjin University(Grant No.2023XZL-0015).
摘要In this work,we coated a layer of Y2O3 particles in Hastelloy X(HX)nickel-based superalloy powder by in situ chemical method and combined with laser powder bed fusion(LPBF)technology to develop a high-performance Y2O3-doping alloy,designated as Y-HX.The results show that the doping of Y2O3 particles prevents crack formation during the printing process and reduces solute segregation at cell and grain boundaries by increasing the viscosity of the molten pool.The doping of Y2O3 particles to the printed Y-HX alloy enhances grain boundary characteristics,transforming coarse sheet-like carbides into finely dispersed granular carbides at the boundaries during subsequent heat treatment.Additionally,doping with Y2O3 particles increases the recrystallization activation energy of the Y-HX alloy from 149.4 to 278.8 kJ mol-1.At 750℃,the Y-HX alloy exhibits an ultimate tensile strength of 619±2 MPa and an elongation of 52%±2%,along with an ultimate tensile strength of 325±3 MPa and an elongation of 47%±2% at 900℃.Our work provides a promising way to develop additive-manufactured superalloys with exceptional thermal stability and remarkable high-temperature mechanical properties.
基金supported by the National Natural Science Foundation of China (No.90411020)Major State Basic Research Development Program of China (973 Program)(2002CB412502).
摘要A study was conducted to determine the effects of elevated CO2 on soil N process at Changbai Mountain in Jilin Province, northeastern China (42°24"N, 128°06"E, and 738 m elevation). A randomized complete block design of ambient and elevated CO2 was established in an open-top chamber facility in the spring of 1999. Changpai Scotch pine (Pinus sylvestris var. sylvestriformis seeds were sowed in May, 1999 and CO2 fumigation treatments began after seeds germination. In each year, the exposure started at the end of April and stopped at the end of October. Soil samples were collected in June and August 2006 and in June 2007, and soil nitrifying, denitrifying and N2-fixing enzyme activities were measured. Results show that soil nitrifying enzyme activities (NEA) in the 5-10 cm soil layer were significantly increased at elevated CO2 by 30.3% in June 2006, by 30.9% in August 2006 and by 11.3% in June 2007. Soil denitrifying enzyme activities (DEA) were significantly decreased by elevated CO2 treatment in June 2006 (P 〈 0.012) and August 2006 (P 〈 0.005) samplings in our study; no significant difference was detected in June 2007, and no significant changes in N2-fixing enzyme activity were found. This study suggests that elevated CO2 can alter soil nitrifying enzyme and denitrifying enzyme activities.
基金financially supported by the National Natural Science Foundation of China(31370425,61273329)the Specialized Research Fund for the Doctoral Program of Higher Education,China(20130204110024)
摘要Global environmental change affects plant physiological and ecosystem processes. The interaction of elevated CO2, drought and nitrogen (N) deficiency result in complex responses of C4 species photosynthetic process that challenge our current understanding. An experiment of maize (Zea mays L.) involving CO2 concentrations (380 or 750 μmol mol1, climate chamber), osmotic stresses (10% PEG-6000, -0.32 MPa) and nitrogen constraints (N deficiency treated since the 144th drought hour) was carried out to investigate its photosynthesis capacity and leaf nitrogen use efficiency. Elevated CO2 could alleviate drought-induced photosynthetic limitation through increasing capacity of PEPC carboxylation (Vp~,x) and decreasing stomatal limitations (SL). The N deficiency exacerbated drought-induced photosynthesis limitations in ambient CO2. Elevated CO2 partially alleviated the limitation induced by drought and N deficiency through improving the capacity of Rubisco carboxylation (Vmax) and decreasing SL. Plants with N deficiency transported more N to their leaves at elevated CO2, leading to a high photosynthetic nitrogen-use efficiency but low whole-plant nitrogen-use efficiency. The stress mitigation by elevated CO2 under N deficiency conditions was not enough to improving plant N use efficiency and biomass accumulation. The study demonstrated that elevated CO2 could alleviate drought-induced photosynthesis limitation, but the alleviation varied with N supplies.
基金Project supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (No.KZCX2-YW-416)National NaturM Science Foundation of China (No.90411020)
摘要The objectives of this study were to investigate the effect of higher CO2 concentrations (500 and 700 μmol mol^-1) in atmosphere on total soil respiration and the contribution of root respiration to total soil respiration during seedling growth of Pinus sylvestris vat. sylvestriformis. During the four growing seasons (May-October) from 1999 to 2003, the seedlings were exposed to elevated concentrations of CO2 in open-top chambers. The total soil respiration and contribution of root respiration were measured using an LI-6400-09 soil CO2 flux chamber on June 15 and October 8, 2003. To separate root respiration from total soil respiration, three PVC cylinders were inserted approximately 30 cm deep into the soil in each chamber. There were marked diurnal changes in air and soil temperatures on June 15. Both the total soil respiration and the soil respiration without roots showed a strong diurnal pattern, increasing from before sunrise to about 14:00 in the afternoon and then decreasing before the next sunrise. No increase in the mean total soil respiration and mean soil respiration with roots severed was observed under the elevated CO2 treatments on June 15, 2003, as compared to the open field and control chamber with ambient CO2. However, on October 8, 2003, the total soil respiration and soil respiration with roots severed in the open field were lower than those in the control and elevated CO2 chambers. The mean contribution of root respiration measured on June 15, 2003, ranged from 8.3% to 30.5% and on October 8, 2003, from 20.6% to 48.6%.
基金supported by the National Key R&D Program of China(2023YFC2604500)the National Natural Science Foundation of China(32171662,32471753 and 32171666)the Natural Science Foundation of Liaoning(2020-MS-199).
摘要Exotic plant invasions and increased atmospheric carbon dioxide(CO2)concentration have been determined to independently affect soil nematodes,a key component of soil biota.However,little is known about the long-term effects of these two global change factors and their interactive effects.Over three consecutive years,we cultivated invasive alien plant Xanthium strumarium and its two phylogenetically related natives under both ambient(aCO2)and elevated(eCO2)atmospheric CO2concentrations,and determined the effects of the invader and natives on soil nematodes under different CO2concentrations and the relevant mechanism.The abundance of total soil nematodes and that of the dominant trophic group(herbivores)were significantly affected by plant species and CO2concentration,and these effects were dependent on the experimental duration,however,the Shannon-diversity of nematodes was not affected by these factors.Under aCO2,both invasive and native species significantly increased the total nematode abundance and that of the dominant trophic group with increasing experimental duration,and the amplitude of the increase was greater under the invader relative to the natives.The eCO2increased total nematode abundance(second year)and that of the dominant trophic group(third year)under the invader,but not under the natives(or even decreased)with increasing experimental duration.Root litter had greater effects on soil nematode abundance than leaf litter and root exudates did.This study indicates that eCO2would aggravate effects of invasive plants on soil nematodes by increasing abundance,and these effects would vary with the duration.
基金financially supported by the National Natural Science Foundation of China (31501276 and 31370425)the Ph D Research Startup Foundation of Shanxi Agricultural University,China (2013YT05)the Specialized Research Fund for the Doctoral Program of Higher Education,China (20130204110024)
摘要The maintenance of rapid growth under conditions of CO2 enrichment is directly related to the capacity of new leaves to use or store the additional assimilated carbon (C) and nitrogen (N). Under drought conditions, however, less is known about C and N transport in C4 plants and the contributions of these processes to new foliar growth. We measured the patterns of C and N accumulation in maize (Zea mays L.) seedlings using 13C and 15N as tracers in CO2 climate chambers (380 or 750 μmol mol-1) under a mild drought stress induced with 10% PEG-6000. The drought stress under ambient conditions decreased the biomass production of the maize plants; however, this effect was reduced under elevated CO2. Compared with the water-stressed maize plants under atmospheric CO2, the treatment that combined elevated CO2 with water stress increased the accumulation of biomass, partitioned more C and N to new leaves as well as enhanced the carbon resource in ageing leaves and the carbon pool in new leaves. However, the C counterflow capability of the roots decreased. The elevated CO2 increased the time needed for newly acquired N to be present in the roots and increased the proportion of new N in the leaves. The maize plants supported the development of new leaves at elevated CO2 by altering the transport and remobilization of C and N. Under drought conditions, the increased activity of new leaves in relation to the storage of C and N sustained the enhanced growth of these plants under elevated CO2.
摘要Elevated,rectangular water tanks are an essential component of the water supply network and for emergency water storage.However,determining the dynamic response from seismic loads will require accurate estimates of their natural frequencies to help avoid damage or failure.Several numerical and analytical methodologies depend on assumptions that may not account for the complexity of fluid-structure interaction regarding rectangular geometries,indicating a strong need for solid experimental validation.This study pioneers the application of Digital Image Correlation(DIC),a non-contact optical technique,to measure the natural frequencies of a small-scale acrylic and Teflon tank,addressing this gap with a novel experimental approach.The method includes DIC using a high-speed camera,image analyzed by MATLAB,frequency analysis by Continuous Wavelet Transformation(CWT),and Fast Fourier Transformation(FFT);ANSYS finite element analysis,simplified models based on Eurocode and the Egyptian Code of Practice(ECP).Study results indicate that DIC is critical for attaining high accuracy,with maximum error differing by 2.92% for impulsive and 4.55%for convective frequencies from ANSYS and provides a better measure of dynamic response compared to contact-based measurements.Impulsive frequency decreased from 5.8724 Hz to 4.0085 Hz,and sloshing increased from 1.00 Hz to 1.84 Hz,as the water cover height varied from 0 cm to 9.8 cm.The Eurocode and ECP models describe acceptable errors of 7.36% and 9.21%,respectively.DIC showed higher accuracy,making it a useful tool for seismic design.This study improves the safety and reliability of designs for elevated water tanks in seismic regions with elevated seismic risk.