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Molecular insights into genetic transformation and somatic embryogenesis in cotton(Gossypium hirsutum L.) 认领 引用
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作者 AYUBOV Mirzakamol SHERMATOV Shukhrat +4 位作者 MIRZAKHMEDOV Mukhammad UBAYDULLAEVA Khurshida OBIDOV Nurdinjon BURIEV Zabardast ABDURAKHMONOV Ibrokhim 《Journal of Cotton Research》 CAS CSCD 2026年第2期281-289,共9页
Genetic transformation and somatic embryogenesis(SE)are essential biotechnological tools for cotton improvement.Recent advances in transcriptomics,proteomics,epigenetics,and RNA interference(RNAi)have enabled us to be... Genetic transformation and somatic embryogenesis(SE)are essential biotechnological tools for cotton improvement.Recent advances in transcriptomics,proteomics,epigenetics,and RNA interference(RNAi)have enabled us to better understand the SE regulatory pathways.This review summarizes the findings of several key research studies,focusing on the roles of transcription factors(e.g.,GhAGL15s,GhL1L1,GhWOX),hormonal signaling(auxin and cytokinin),DNA methylation,and small RNAs in SE initiation and embryogenic callus formation.In addition,we discuss recent breakthroughs in Agrobacterium-mediated and RNAi-based transformation.Challenges such as genotype recalcitrance and somaclonal variation,along with new approaches to improve SE efficiency,are also addressed. 展开更多
关键词 Somatic embryogenesis Hormonal signaling Agrobacterium-mediated transformation Transcriptomics RNA interference
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Establishment of somatic embryogenesis regeneration system and transcriptome analysis of early somatic embryogenesis in litchi 认领 引用 被引量：9
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作者 Yaqi Qin Bo Zhang +5 位作者 Shiqian Wang Wuyan Guo Zhike Zhang Yonghua Qin Jietang Zhao Guibing Hu 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第2期535-547,共13页
Litchi chinensis Sonn.is an important economic fruit tree in tropical and subtropical regions.Regrettably,the efficiency of plant regeneration via somatic embryogenesis in litchi is typically low due to the poor conve... Litchi chinensis Sonn.is an important economic fruit tree in tropical and subtropical regions.Regrettably,the efficiency of plant regeneration via somatic embryogenesis in litchi is typically low due to the poor conversion of embryos to plants.The purpose of this study was to establish a regeneration system via somatic embryogenesis from immature embryos explants in‘Heiye'cultivar of litchi.Our results demonstrated that MS medium supplemented with 2.0 mg L-12,4-D was optimal for callus induction.For somatic embryo(SE)induction,MS medium containing0.5 g L-1 activated charcoal(AC)was the most effective,while the use of zeatin(ZT)and thidiazuron(TDZ)resulted in abnormal somatic embryos.The rooting and regeneration rate of 2.15%and 17.5%,respectively,were achieved using MS medium supplemented with 0.5 g L-1 AC.Furthermore,transcriptome analysis was performed on embryogenic callus(EC),globular embryo(GE),and heart embryo(HE)to explore the molecular mechanisms of early somatic embryogenesis.2,587 common differentially expressed genes(DEGs)between EC_vs_GE and EC_vs_HE were identified,and the expression patterns of these common DEGs were separated into twelve major clusters.GO annotation and KEGG pathway analysis revealed that these common DEGs were implicated in plant hormone signal transduction,auxin-activated signaling pathway,and other biological processes.Additionally,differentially expressed transcription factors were identified,and the function of LcBBM2 which is specifically highly expressed during early somatic embryogenesis was verified.Overexpression of LcBBM2 in tomato promotes callus and shoot formation.Therefore,this study can provide a theoretical basis and technical support for genetic breeding improvement of litchi. 展开更多
关键词 Litchi chinensis Somatic embryogenesis Transcriptome LcBBM2
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Genome-wide analysis and stress-responsive expression profiling of the LEA (late embryogenesis abundant) gene family in wild peanut 认领 引用
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作者 Jiang Huanhuan Chen Qiangbin +1 位作者 Wang Tong Chen Gang 《Oil Crop Science》 CAS CSCD 2025年第2期100-108,共9页
Late embryogenesis abundant (LEA) proteins generally accumulate in seeds during the later stages of maturation.Here we studied the LEA genes in two wild peanut species (Arachis duranensis and Arachis ipaensis) in an e... Late embryogenesis abundant (LEA) proteins generally accumulate in seeds during the later stages of maturation.Here we studied the LEA genes in two wild peanut species (Arachis duranensis and Arachis ipaensis) in an effort to create a genetic resource for peanut crop improvement.we identified 65 AdLEA and 69 AiLEA genes representing all 8 LEA subfamilies,which were unevenly distributed across 10 peanut chromosomes.The majority of LEA proteins were found to be highly hydrophilic.MEME analysis indicated that LEA gene motifs were conserved within groups,but not between groups.The LEA genes contained a diverse array of stress-and phytohormoneresponsive cis-acting elements,with the AdLEA2-20 and AiLEA2-20 genes containing the greatest number of elements.Both AdLEA2-20 and AiLEA2-20 were upregulated in response to cold temperatures,drought,salinity,and abscisic acid exposure,although the dynamics were tissue-dependent.This study lays the foundation for future studies on the LEA gene family and abiotic stress in peanut,and our results will be invaluable for the genetic improvement of peanut by characterizing the genetic resources of wild peanut species. 展开更多
关键词 Genome-wide analysis Peanut Late embryogenesis abundant Stress
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Expression dynamics of WOX homeodomain transcription factors during somatic embryogenesis in Liriodendron hybrids 认领 引用 被引量：9
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作者 Xiaofei Long Jiaji Zhang +7 位作者 Dandan Wang Yuhao Weng Siqin Liu Meiping Li Zhaodong Hao Tielong Cheng Jisen Shi Jinhui Chen 《Forestry Research》 2023年第1期158-169,共12页
The relict woody plant genus Liriodendron contains two endangered species,Liriodendron chinense and Liriodendron tulipifera.Understanding the molecular mechanisms involved in early embryo development is important for ... The relict woody plant genus Liriodendron contains two endangered species,Liriodendron chinense and Liriodendron tulipifera.Understanding the molecular mechanisms involved in early embryo development is important for horticultural and ecological research,particularly for the development of improved somatic embryogenesis systems.However,the specific molecular processes underlying embryogenesis in these species remain largely unexplored.To address this,we investigated expression of the WOX(WUSCHEL-related homeobox)gene family of transcription factors throughout somatic embryogenesis.We confirmed expression of eight out of 11 novel candidate LcWOX genes in L.chinense using qRT-PCR and examined spatiotemporal expression patterns of the expressed genes using stable reporter lines that had been transformed with different LcWOX promoters driving GUS expression.We observed embryo developmental stages and expression patterns that broadly correlated with those reported for Arabidopsis somatic embryogenesis.LcWUS was weakly expressed during the transition stage and was predominantly restricted to the apical meristem.LcWOX5 was specifically expressed in the root meristem and restricted to the cotyledons thereafter,and LcWOX4 expression was restricted to the vascular tissue of cotyledonary embryos.In contrast,LcWOX9 was expressed in the embryonic callus and the entire embryonic cell mass,then became restricted to the basal cells,indicating a potential role in regulating embryonic maintenance.Our findings provide insights into spatiotemporally specific WOX transcription and shed new light on potential functions of WOX genes during Liriodendron somatic embryogenesis. 展开更多
关键词 WOX transcription factors development improved somatic embryogenesis systemshoweverthe somatic embryogenesis molecular processes Liriodendron hybrids spatiotemporal expression embryonic development
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Genome-wide identification and expression analysis of late embryogenesis abundant(LEA)genes reveal their potential roles in somatic embryogenesis in hybrid sweetgum(Liquidambar styraciflua×Liquidambar formosana) 认领 引用 被引量：1
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作者 Ying Li Shuaizheng Qi +7 位作者 Siyuan Chen Hongxuan Li Ting Zhang Fen Bao Dingju Zhan Zhenwu Pang Jinfeng Zhang Jian Zhao 《Forestry Research》 2023年第1期194-208,共15页
Late embryogenesis abundant(LEA)proteins are widely distributed in higher plants that play significant roles in embryonic development and abiotic stress response.Hybrid sweetgum is an important forest tree resource ar... Late embryogenesis abundant(LEA)proteins are widely distributed in higher plants that play significant roles in embryonic development and abiotic stress response.Hybrid sweetgum is an important forest tree resource around the world,and somatic embryogenesis is an efficient way of reproduction and utilization.However,a systematic analysis of the LEA family genes in hybrid sweetgum is lacking,this is not conducive to the efficiency of its somatic embryogenesis.From the whole genome of the hybrid sweetgum,utilizing hidden Markov models,an identification of a total of 79 LEA genes was successfully conducted.They were classified into eight different groups based on their conserved domains and phylogenetic relationships,with the LsfLEA2 group of genes being the most abundant.The gene structure and sequence characteristics and chromosomal localization,as well as the physicochemical properties of LEA proteins were meticulously carried out.Analysis of the cis-acting elements shows that most of the LsfLEA genes are associated with light-responsive-elements.In addition,some genes are associated with biosynthetic pathways,such as abscisic acid response,growth hormone response,methyl jasmonate response,somatic embryogenesis,meristematic tissue expression.Furthermore,we systematically analyzed the expression patterns of hybrid sweetgum LEA genes in different stages of somatic embryogenesis and different tissues,in LEA family genes we also found significant specificity in gene expression during somatic embryogenesis.This study provides new insights into the formation of members of the LsfLEA family genes in hybrid sweetgum,while improving the understanding of the potential role of these genes in the process of hybrid sweetgum somatic embryogenesis and abiotic stress response.These results have a certain guiding significance for the future functional study of LsfLEA family genes,and provide a theoretical basis for exploring the regulatory mechanism of LsfLEA genes in the somatic embryo development stage of hybrid sweetgum. 展开更多
关键词 hybrid sweetgum higher plants somatic embryogenesis forest tree resource embryonic development genome wide identification expression analysis somatic embryogenesisfrom
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Selection of culture conditions for callus induction and proliferation by somatic embryogenesis of Pinus koraiensis 认领 引用 被引量：18
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作者 Fang Gao Chunxue Peng +2 位作者 Hao Wang Hailong Shen Ling Yang 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第2期483-491,共9页
The induction and proliferation of embryogenic callus are key steps for large-scale propagation of somatic embryogenesis pathway and long-term preservation of coniferous germplasm.Callus can be induced from immature e... The induction and proliferation of embryogenic callus are key steps for large-scale propagation of somatic embryogenesis pathway and long-term preservation of coniferous germplasm.Callus can be induced from immature embryos of Korean pine(Pinus koraiensis Sieb.et Zucc.;Pinaceae)as explants,but there are problems,such as low proliferation efficiency,loss of embryogenicity,poor vigor;thus,best conditions for proliferation and culture of immature embryos of Korean pine are not yet clear.To solve the problems with somatic embryogenesis of Korean pine and determine the best culture conditions for callus induction and proliferation,we varied hormone concentration,subculture cycle of proliferation and other plant growth regulators combinations in media to induce callus formation by megagametophytes of three Korean pine families at different developmental stages,then analyzed the effects on embryogenic callus retention and cell proliferation using a quadratic regression orthogonal rotation design.The results showed that the family origin and collection date of explants significantly affected callus induction(induction rate reached 1.67%).Embryogenic maintenance and callus proliferation were best on DCR medium supplemented with 0.25 mg L−16-benzyl adenine,1 mg L−1naphthaleneacetic acid,30 g L−1sucrose,500 mg L−1,L-glutamine,500 mg L−1casein hydrolysis and 6.5 g L−1agar.In addition,the combination of 2,4-dichlorophenoxyacetic acid+6-benzyl adenine also had a better proliferative effect on callus.The effects of different combinations of growth regulators on callus proliferation efficiency were significantly different.Transfer to new medium every 13–15 days not only maintained robust callus vigor,but also yielded a larger proliferation coefficient.The techniques and conditions for embryogenic callus induction and proliferation of Korean determined here will serve as a foundation for establishing a large-scale system for somatic embryogenesis and propagation of Korean pine. 展开更多
关键词 Korean pine Somatic embryogenesis Embryogenic callus Megagametophytes Embryonic maintenance
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The paternal epigenome and embryogenesis: poising mechanisms for development 认领 引用 被引量：16
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作者 Timothy G Jenkins Douglas T Carrell 《Asian Journal of Andrology》 SCIE CAS CSCD 2011年第1期76-80,共5页
The scope of paternal contributions during early embryonic development has long been considered limited. Dramatic changes in chromatin structure throughout spermatogenesis have been thought to leave the sperm void of ... The scope of paternal contributions during early embryonic development has long been considered limited. Dramatic changes in chromatin structure throughout spermatogenesis have been thought to leave the sperm void of complex layers of epigenetic regulation over the DNA blueprint, thus leaving the balance of that regulation to the oocyte. However, recent work in the fields of epigenetics and male factor infertility has placed this long-held, and now controversial dogma, in a new light. Elegant studies investigating chromatin and epigenetic modifications in the developing sperm cell have provided new insights that may establish a more critical role for the paternal epigenome in the developing embryo. DNA methylation, histone tail modifications, targeted histone retention and protamine incorporation into the chromatin have great influence in the developing sperm cell. Perturbations in the establishment and/or maintenance of any of these epigenetic marks have been demonstrated to affect fertility status, ranging in severity from mild to catastrophic. Sperm require this myriad of chromatin structural changes not only to serve a protective role to DNA throughout spermatogenesis and future delivery to the egg, but also, it appears, to contribute to the developmental program of the future embryo. This review will focus on our current understanding of the epigenetics of sperm. We will discuss sperm-specific chromatin modifications that result in genes essential to development being poised for activation early in embryonic development, the disruption of which may result in reduced fecundity. 展开更多
关键词 chromatin DNA methylation embryogenesis epigenetics histone modification male infertility
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Characterization of Gh SERK2 and its expression associated with somatic embryogenesis and hormones level in Upland cotton 认领 引用 被引量：4
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作者 LIU Zheng-jie ZHAO Yan-peng +3 位作者 ZENG Ling-he ZHANG Yuan WANG Yu-mei HUA Jin-ping 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第3期517-529,共13页
Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function... Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function in SE. A homolog GhSERK2 （accession number： JF430801） was cloned from Upland cotton and characterized for its functions in SE. GhSERK2 expressed in different tissues and showed higher expression level in floral organs than vegetative ones with the highest levels in ovule and anther. GhSERK2 expressed during SE with a high level at globular embryos stage. Upon treatment with indole-3-butytic acid （IBA）, the transcription level of GhSERK2 was induced and promoted SE subsequently. A 2-day treatment of 2,4-dichlorophenoxyacetic acid （2,4-D） induced the expression of GhSERK2, but treatments of 2,4-D for longer periods sharply inhibited the GhSERK2 transcription level of embryogenic callus （EC）. The levels of hormones, including 3-indoleacetic acid （IAA）, abscisic acid （ABA）, and brassinosteroid （BR）, were increased in the initial calli induced from the over-expression of GhSERK2 cotton. Our results indicated that GhSERK2 expression was associated with induction of SE and closely related to hormone levels during tissue culture in Upland cotton, and the gene might play an important role in regeneration of cotton. 展开更多
关键词 GhSERK2 somatic embryogenesis hormone level expression pattern Upland cotton （Gossypium hirsutum L.）
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Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’ 认领 引用 被引量：4
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作者 Jingjue Zeng Yi Deng +8 位作者 Shahid Iqbal Jiarui Zhang Kunlin Wu Guohua Ma Lin Li Guangyi Dai Rufang Deng Lin Fang Songjun Zeng 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期556-572,共17页
Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis(SE) is an efficient method for mass production of Hippeastrum plantlets. Previo... Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis(SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the SE and its regulatory networks are rarely reported. In this study, we established a direct SE method of Hippeastrum Bangkok Rose' using leaf bases as explants. MS supplemented with 1.00 mg·L-1NAA +1.00 mg·L-1KT + 0.25 mg·L-1TDZ was the optimal medium for SE. Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation,globular, scutellar and coleoptile stages. During SE, endogenous hormones of IAA, CTK, ABA, and SA were highly accumulated. Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced, while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated, respectively, leading to suppression of auxin signaling. In contrast, cytokine signaling was promoted at the early stage of SE, as biosynthesis, transport, and signaling components were up-regulated.Various stress-related genes were up-regulated at the early or late stages of SE. Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE. Moreover, key SE regulators, including WOXs and SERKs were highly expressed along with SE. Overall, the present study provides insights into the SE regulatory mechanisms of the Hippeastrum. 展开更多
关键词 Hippeastrum Tissue culture Somatic embryogenesis Gene regulation
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Comparative transcriptome study provides insights into acquisition of embryogenic ability in upland cotton during somatic embryogenesis 认领 引用 被引量：5
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作者 SUN Ruibin TIAN Ruiping +2 位作者 MA Dan WANG Shaohui LIU Chuanliang 《Journal of Cotton Research》 CAS 2018年第2期44-56,共13页
Background： The conversion from non-embryogenic callus （NEC） to embryogenic callus （EC） is the key bottleneck step in regeneration of upland cotton （Gossypium hirsutum）, and hinders the transgenic breeding of u... Background： The conversion from non-embryogenic callus （NEC） to embryogenic callus （EC） is the key bottleneck step in regeneration of upland cotton （Gossypium hirsutum）, and hinders the transgenic breeding of upland cotton. To investigate molecular mechanisms underlying acquisition of embryogenic potential during this process, comparation analysis of transcriptome dynamics between two upland cotton cultivars with different somatic embryogenesis abilities was conducted. Results： Differentially expressed genes involved in the transformation from NEC to EC were detected in the two different cultivars. Principal component analysis based on DEGs showed that the NEC tissues of the two cultivars were highly heterogeneous, whereas the derived EC tissues were similar, which suggested the homogeneousness of EC between different lines. In the highly embryogenic cultivar CCRI 24, more of these genes were down-regulated, whereas, in the recalcitrant cultivar CCRI 12, more were up-regulated. Bioinformatics analysis on these DEGs showed that the vast majority of differentially expressed genes were enriched in metabolism and secondary metabolites biosynthesis pathways. Flavonoid biosynthesis and phenylpropanoid biosynthesis pathways were enriched in both cultivars, and the associated genes were down-regulated more in CCRI 24 than in CCRI 12. We deduced that vigorous secondary metabolism in CCRI 12 may hinder primary metabolism, resulting in tardiness of cell differentiation. Interestingly, genes involved in the plant hormone signal transduction pathway were enriched in the recalcitrant cultivar CCRI 12, but not in CCRI 24, suggesting more radical regulation of hormone signal transduction in the recalcitrant cultivar. Signal transduction rather than biosynthesis of plant hormones is more likely to be the determining factor triggering NEC to EC transition in recalcitrant cotton lines. Transcription factor encoding genes showed differential regulation between two cultivars. Conclusions： Our study provides valuable information about the molecular mechanism of conversion from NEC to EC in cotton and allows for identification of novel genes involved. By comparing transcriptome changes in transformation from NEC to EC between the two cultivars, we identified 46 transcripts that may contribute to initiating embryogenic shift. 展开更多
关键词 Upland cotton Transcriptome Non-embryogenic callus （NEC） Embryogenic callus （EC） Somatic embryogenesis
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Transcriptomic and physiological analysis identifies a gene network module highly associated with brassinosteroid regulation in hybrid sweetgum tissues differing in the capability of somatic embryogenesis 认领 引用 被引量：3
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作者 Ruirui Zhao Shuaizheng Qi +5 位作者 Ying Cui Ying Gao Shuaifei Jiang Jian Zhao Jinfeng Zhang Lisheng Kong 《Horticulture Research》 SCIE CSCD 2022年第1期449-460,共12页
Somatic embryogenesis is a preferred method for large-scale production of forest trees due to its high propagation efficiency.In this study,hybrid sweetgum leaves with phase changes from mature to embryogenic state we... Somatic embryogenesis is a preferred method for large-scale production of forest trees due to its high propagation efficiency.In this study,hybrid sweetgum leaves with phase changes from mature to embryogenic state were selected as experimental material to study somatic embryo initiation.Embryogenicity ranged from high to low,i.e.from 45%,25%,and 12.5%to 0,with the samples of embryogenic callus(EC),whiten leaf edge(WLI),whiten leaf(WLII),and green leaf(GL)respectively.High correlations existed between embryogenicity and endogenous brassinosteroids(BRs)(r=0.95,p<0.05).Similarly,concentrations of endogenous BRs of the sample set correlated positively(r=0.93,0.99,0.87,0.99,0.96 respectively,P<0.05)to expression of somatic embryo(SE)-related genes,i.e.BBM,LEC2,ABI3,PLT2,and WOX2.Hierarchical cluster and weighted gene coexpression network analysis identifiedmodules of coexpressed genes and network in 4820 differentially expressed genes(DEGs)from All-BR-Regulated Genes(ABRG).Moreover,exogenously-supplemented epiBR,together with 2,4-D and 6-BA,increased embryogenicity of GL-sourced callus,and expression of SE-and auxin-related genes,while brassinazole(BRZ),a BR biosynthesis inhibitor,reduced embryogenicity.Evidences obtained in this study revealed that BRs involved in phase change of leaf explants andmay function in regulating gene expression and enhancing auxin effects.This study successfully established protocols for inducing somatic embryogenesis from leaf explants in hybrid sweetgum,which could facilitate the propagation process greatly,and provide theoretical basis for manipulating SE competence of explants in ornamental woody plants. 展开更多
关键词 forest trees physiological analysis brassinosteroid regulation somatic embryogenesis sweetgum leaves transcriptomic analysis somatic embryo initiationembryogenicity hybrid sweetgum
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Cloning and Characterization of a Somatic Embryogenesis Receptor-Like Kinase Gene in Cotton (Gossypium hirsutum) 认领 引用 被引量：5
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作者 SHI Ya-li ZHANG Rui +2 位作者 WU Xiao-ping MENG Zhi-gang GUO San-dui 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第6期898-909,共12页
A novel gene, GhSERK1, was identified in cotton. It encoded a protein belonging to the somatic embryogenesis receptor- like kinase （SERK） family. The genomic sequence of GhSERK1 was 6 920 bp in length, containing a ... A novel gene, GhSERK1, was identified in cotton. It encoded a protein belonging to the somatic embryogenesis receptor- like kinase （SERK） family. The genomic sequence of GhSERK1 was 6 920 bp in length, containing a predicted transcriptional start site （TSS）. Its full-length cDNA was 2 502 bp, encoding a protein of 627 amino acids. Sequence analysis of GhSERK1 revealed high levels of similarity to other reported SERKs, as well as a conserved intron/exon structure that was unique to members of the SERK family. Expression analysis showed that GhSERK1 mRNA was present in all organs of cotton plants and at different developmental stages, but its transcripts were most abundant in reproductive organs. Compared with that of the male-fertile line, the level of GhSERK1 mRNA was lower in the anther of the male-sterile cotton line, in which the pollen development was defected. Taken together, these findings illustrated that the GhSERK1 play a critical role during the anther formation, and may also have a broad role in other aspects of plant development. 展开更多
关键词 cotton （Gossypium hirsutum） somatic embryogenesis receptor-like kinase （SERK） reproductive organs,anther development
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Exogenous Spermidine Promotes Somatic Embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content 认领 引用 被引量：4
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作者 Dandan Wang Yulin Guo +7 位作者 Xiaofei Long Yan Pan Dingjie Yang Rui Li Ye Lu Ying Chen Jisen Shi Jinhui Chen 《Phyton-International Journal of Experimental Botany》 SCIE 2020年第1期27-34,共8页
In order to study how exogenous hormones in C.lanceolata(gymnosperm)regulate somatic embryogenesis,we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found th... In order to study how exogenous hormones in C.lanceolata(gymnosperm)regulate somatic embryogenesis,we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic embryogenesis.By applying exogenous spermidine,we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormone content.Based on these results,further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic embryogenesis.Our research may benefit the long-term economic output of the forestry industry and lays the foundation to studying the molecular mechanism that controls somatic embryogenesis efficiency. 展开更多
关键词 Spermidine C.lanceolata somatic embryogenesis endogenous phytohormones
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Relationship between H2O2 accumulation and NO synthesis during osmotic stress:promoted somatic embryogenesis of Fraxinus mandshurica 认领 引用 被引量：2
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作者 Ling Yang Hanyang Guo +3 位作者 Yingying Liu Dongyan Zhang Hongnan Liu Hailong Shen 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第3期917-925,共9页
Osmotic stress promotes somatic embryogenesis of Fraxinus mandshurica,which leads to accumulation of reactive oxygen species(ROS).The single pieces of cotyledons of F.mandshurica were used as explants to induce somati... Osmotic stress promotes somatic embryogenesis of Fraxinus mandshurica,which leads to accumulation of reactive oxygen species(ROS).The single pieces of cotyledons of F.mandshurica were used as explants to induce somatic embryogenesis in osmotic-stress medium.Furthermore,the hydrogen peroxide H2O2 content of explanted cells was varied by adding exogenous H2O2 or catalase solution to assess the effects of the exogenous H2O2on somatic embryogenesis,intracellular H2O2accumulation,and the relationship between signaling mediated by ROS or reactive nitrogen species.The results revealed that exogenous H2O2(100?300μmol L–1)increased the number of somatic embryos.On 60th day of exogenous H2O2(200μmol L–1)treatment,the number of somatic embryos of explants treated,which was 136.54%,was higher than the control.Moreover,exogenous H2O2(100μmol L–1)significantly increased the intracellular H2O2content and enhanced the activities of superoxidase dismutase and peroxidase.Finally,exogenous H2O2(100μmol L–1)activated the intracellular non-enzymatic pathway for nitric oxide(NO)synthesis.The somatic embryogenesis in broadleaf trees increases with the change of endogenic ROS content,and depends on the upregulation of antioxidant enzymes.Both H2O2and NO,as signaling molecules,were found to be involved in the process of somatic embryogenesis in broadleaf trees.In the process of exogenous H2O2promoting somatic embryogenesis,NO synthesis depended on non-enzymatic reactions.These results provide a scientific basis for resolving the mechanism by which ROS levels are regulated during somatic embryogenesis of broadleaf trees and establish a reasonable and efficient technology system for regulating somatic embryogenesis of trees. 展开更多
关键词 Fraxinus mandshurica Somatic embryogenesis Hydrogen peroxide Nitric oxide Osmotic stress
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Reactive oxygen species, nitric oxide and plant cell death associated with caspase-like protease activity during somatic embryogenesis in Fraxinus mandshurica 认领 引用 被引量：2
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作者 Hao Wang Dongyan Zhang +2 位作者 Juan Luis Fernández-Lorenzo Hailong Shen Ling Yang 《Journal of Forestry Research》 SCIE CAS CSCD 2022年第3期1005-1017,共13页
Programmed cell death occurs in browning explants of Fraxinus mandshurica during somatic embryogenesis, but the underlying mechanism is unclear. In this study, single cotyledons of zygotic embryos of F. mandshurica we... Programmed cell death occurs in browning explants of Fraxinus mandshurica during somatic embryogenesis, but the underlying mechanism is unclear. In this study, single cotyledons of zygotic embryos of F. mandshurica were used as explants. Mitochondrial structure and function, caspase-3-like protease activity, hydrogen peroxide metabolism, and nitric oxide accumulation induced by high concentrations of sucrose and plant growth regulators were studied. The results show that plant growth regulators induced somatic embryogenesis and also promoted explant browning. High sucrose concentrations had similar effects. High concentrations of sucrose and plant growth regulators led to the accumulation of hydrogen peroxide and nitric oxide which induced changes in mitochondrial structure and function such as modifications in mitochondrial morphology, increased membrane permeability, decreased membrane potential, and the release of cytochrome c into the cytoplasm. An increase in caspase-3-like protease activity triggered programmed cell death in some browning explant cells. During somatic embryogenesis there were increased activities of superoxide dismutase, peroxidase, and catalase, which are associated with hydrogen peroxide metabolism and jointly maintain reactive oxygen species levels. Intracellular nitric oxide synthase and nitrate reductase activities were not significantly correlated with nitric oxide content. Instead, intracellular nitric oxide may be derived from non-enzymatic reactions. Our results indicate that hydrogen peroxide and nitric oxide may function as signals, playing key roles in somatic embryogenesis and programmed cell death of explant cells of F. mandshurica. The interaction between nitric oxide and reactive oxygen species determines the occurrence of programmed cell death in explant cells;somatic embryogenesis and programmed cell death are positively regulated by hydrogen peroxide. However, the regulation of nitric oxide is complex. 展开更多
关键词 Manchurian ash Somatic embryogenesis Programmed cell death Mitochondria Reactive oxide species Nitric oxide
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Foregut duplication cysts:A report of two cases with emphasis on embryogenesis 认领 引用 被引量：6
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作者 Thaer Khoury Louis Rivera 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第1期130-134,共5页
Duplication cyst of the stomach with a pseudostratifie columnar ciliated epithelium is extremely rare.We de scribe two cases of these cysts,with emphasis on the immunophenotype and embryogenesis.The first patien was a... Duplication cyst of the stomach with a pseudostratifie columnar ciliated epithelium is extremely rare.We de scribe two cases of these cysts,with emphasis on the immunophenotype and embryogenesis.The first patien was a 29-year-old man who presented with crampin abdominal pain in his left lower quadrant.The secon patient was a 26-year-old woman who had a history over several years,of chronic epigastric abdominal pai radiating to her back.Both lesions were surgically re moved.They showed the same histomorphology.Th cysts were lined by a pseudostratified respiratory ep thelium with ciliated cells.The first cyst was connecte to the stomach,while the second cyst was not connect ed.Both cysts expressed thyroid transcription factor(TTF-1) and surfactant.In this report,we explore th possible embryogenesis of these lesions in the light o TTF-1 and surfactant expression. 展开更多
关键词 Duplication cyst Stomach Thyroid transcription factor-1 Surfactant Embryogenesis
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Functional analysis of the eTM-miR171-SCL6 module regulating somatic embryogenesis in Lilium pumilum DC.Fisch 认领 引用 被引量：2
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作者 Rui Yan Shengli Song +1 位作者 Hongyu Li Hongmei Sun 《Horticulture Research》 SCIE CSCD 2022年第1期2103-2116,共14页
Somatic embryogenesis(SE)is of great significance in Lilium bulb production,germplasm preservation,and genetic improvement.miRNAs are important regulators of plant growth and development at the transcriptional level.P... Somatic embryogenesis(SE)is of great significance in Lilium bulb production,germplasm preservation,and genetic improvement.miRNAs are important regulators of plant growth and development at the transcriptional level.Previous research by our group has shown that lpu-miR171 and its target gene SCARECROW-LIKE 6(SCL6)play an important regulatory role in lily SE,and we predicted and identified that endogenous target mimics(eTMs)can regulate lpu-miR171.However,the associated mechanism and internal regulatory network are not yet clear.In the present study,lpu-miR171 was used as an entry point to explore the regulatory network between its upstream eTMs and its downstream target gene LpSCL6,as well as to identify the mechanism of this regulatory network in Lilium SE.Tobacco transient transformation confirmed that miRNA171 significantly inhibited the expression of LpSCL6.On this basis,the Lilium stable genetic transformation systemwas used to demonstrate that silencing lpu-miR171a and lpu-miR171b and overexpressing LpSCL6-II and LpSCL6-I promoted starch accumulation in calli and the expression of key cell cycle genes,thus providing energy tomeet preconditions for SE and accelerate the formation and development of Lilium somatic embryos.LpSCL6-II and LpSCL6-I are nuclear proteins with self-activation activity in yeast cells.In addition,we confirmed in Lilium that lpu-eTM171 is the eTM of lpu-miR171 that binds lpu-miR171 to prevent cleavage of the target gene LpSCL6,thereby promoting SE.Therefore,the present study established a new mechanism whereby the eTM-miR171-SCL6 module regulates SE in Lilium pumilum and provides new insights clarifying the mechanism of SE. 展开更多
关键词 miRNA genetic improvementmirnas SCL endogenous target mimics etms can somatic embryogenesis lilium bulb Lilium pumilum endogenous target mimic
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Molecular basis of the first cell fate determination in mouse embryogenesis 认领 引用 被引量：2
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作者 Lingyi Chen Dekun Wang +2 位作者 Zhaoting Wu Liping Ma George Q Daley 《Cell Research》 SCIE CAS 2010年第9期982-993,共12页
Through proliferation and differentiation, a single cell, the zygote, can give rise to a complex organism composed of many types of cells. Up to the eight-cell embryo stage, the blastomeres are morphologically identic... Through proliferation and differentiation, a single cell, the zygote, can give rise to a complex organism composed of many types of cells. Up to the eight-cell embryo stage, the blastomeres are morphologically identical and distributed symmetrically in the mammalian embryo. Functionally, in some species, they are all totipotent. However, due to the compaction of blastomeres and the asymmetrical cell division at the late phase of the eight-cell embryo, the blastomeres of the morula are no longer identical. During the transition from morula to blastocyst, blastomeres differentiate, resulting in the first cell fate decision in embryogenesis, namely, the segregation of the inner cell mass and the tropheetoderm. In this review, we will discuss the regulatory mechanisms essential for the cell fate choice during blastocyst development, including transcriptional regulation, epigenetic regulation, mieroRNAs, and signal transduction. 展开更多
关键词 inner cell mass trophectoderm embryogenesis
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Effect of plant growth regulators on direct somatic embryogenesis in camphor tree(Cinnamomum camphora L.)from immature zygotic embryos and embryogenic calli induction 认领 引用 被引量：3
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作者 Du Li Zhou Suo Bao Man-zhu 《Forestry Studies in China》 2007年第4期267-271,共5页
A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree （Cinnamomum camphora L.） is presented. After a subculture of 2-3 years, embryogenic calli could be... A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree （Cinnamomum camphora L.） is presented. After a subculture of 2-3 years, embryogenic calli could be derived from primary somatic embryos. Immature zygotic embryos were cultured on a Murashige and Skoog （MS） basal medium supplemented with a range of combinations of cytokinins （BA） and auxins （2,4-D or NAA） for somatic embryo induction. Primary somatic embryos could be induced directly in almost all PGR combinations. A positive effect of 2,4-D on somatic embryogenesis from immature zygotic embryos of camphor tree was obtained. BA at appropriate concentrations （〈 5 mg-L-1） had an effect similar to 2,4-D, whereas high concentrations （〉 5 mg·L^-1） of BA had the effect of restraining somatic embryo induction. NAA had a less positive effect on somatic embryogenesis than 2,4-D. 展开更多
关键词 Cinnamomum camphora L. somatic embryogenesis primary somatic embryo secondary somatic embryogenesis em-bryogenic calli
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Histological Study on Soybean Somatic Embryogenesis 认领 引用 被引量：11
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作者 赵桂兰 杨向东 +1 位作者 郭东全 胡赞民 《Agricultural Science & Technology》 CAS 2008年第2期49-53,75,共5页
Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean... Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency, beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation, differentiation and development of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histological study. The result showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic cells were differentiated from epidermis of explant or cells in 1 layer or 2 layers, with the division of embryogenic cells and degradation and disorganization of surrounding cells, the embryogenic cells would form embryoid with analogous suspensor structure. Later, globular embryoid would extrude from epidermis then developed into heart-shape embryo. The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogenesis induced by young cotyledon of soybean. 展开更多
关键词 Soybean Somatic embryogenesis Histology
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