【目的】构建油橄榄四倍体植株,分析其与野生型二倍体植株的表型差异,探索油橄榄四倍体巨大化表型形成的基因调控机制。【方法】使用秋水仙素和安磺灵混合溶液,以滴液法诱导植株,应用流式细胞术快速鉴定植株倍性;通过改良苯酚品红溶液染...【目的】构建油橄榄四倍体植株,分析其与野生型二倍体植株的表型差异,探索油橄榄四倍体巨大化表型形成的基因调控机制。【方法】使用秋水仙素和安磺灵混合溶液,以滴液法诱导植株,应用流式细胞术快速鉴定植株倍性;通过改良苯酚品红溶液染色,检测植株核型;通过振荡切片和甲苯胺蓝溶液染色,观察四倍体植株叶片横切面;通过PI染色,观察四倍体植株叶片表皮细胞;使用Illumina平台对不同倍性的叶片进行基因表达分析和DEGs富集分析;基于T2T油橄榄高质量基因组,利用Blastp、HMM、ProtParam、cello version 2.5、MEGA11、AlphaFold3.0、SAVES、PyMOL等分析平台,分析鉴定EXP基因家族成员,解析其基因表达模式。【结果】共筛选出23株四倍体油橄榄植株,得率为24.46%;四倍体与二倍体相比,植株出现巨大化的表型,表皮细胞和气孔极显著增大(P<0.0001);共筛选到4743个差异基因,其中2371个上调,2372个下调;通过差异基因分析,富集到与细胞巨大化密切相关的生长素信号转导通路,鉴定出2个显著上调的EXP成员,并进行qRT-PCR验证;在全基因组水平上完成了对EXP基因家族的分析。【结论】EXP基因家族与四倍体细胞巨大化密切相关,为今后进一步探索多倍体巨大化表型奠定了基础。展开更多
In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to...In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to be special values, the solitary wave solutions are derived from the exact traveling wave solutions. The validity and reliability of the method are tested by its applications to Nano-ionic solitons wave’s propagation along microtubules in living cells and Nano-ionic currents of MTs which play an important role in biology.展开更多
Objective To investigate the distribution of bra in-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immu...Objective To investigate the distribution of bra in-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, a lso in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulati on of RGCs.展开更多
For the multi-parameter discrete exponential family,we construct an empirical Bayes(EB)estimator of the vector-valued parameterθ.under some conditions,this estimator is proved to be asymptotically optimal.
This paper applies the EXP-function method to find exact solutions of various nonlinear equations. Tzitzeica- Dodd-Bullough (TDB) equation was selected to illustrate the effectiveness and convenience of the suggested ...This paper applies the EXP-function method to find exact solutions of various nonlinear equations. Tzitzeica- Dodd-Bullough (TDB) equation was selected to illustrate the effectiveness and convenience of the suggested method. More generalized solitonary solutions with free parameters were obtained by suitable choice of the free parameters, and also the obtained solitonary solutions can be converted into periodic solutions.展开更多
摘要【目的】构建油橄榄四倍体植株,分析其与野生型二倍体植株的表型差异,探索油橄榄四倍体巨大化表型形成的基因调控机制。【方法】使用秋水仙素和安磺灵混合溶液,以滴液法诱导植株,应用流式细胞术快速鉴定植株倍性;通过改良苯酚品红溶液染色,检测植株核型;通过振荡切片和甲苯胺蓝溶液染色,观察四倍体植株叶片横切面;通过PI染色,观察四倍体植株叶片表皮细胞;使用Illumina平台对不同倍性的叶片进行基因表达分析和DEGs富集分析;基于T2T油橄榄高质量基因组,利用Blastp、HMM、ProtParam、cello version 2.5、MEGA11、AlphaFold3.0、SAVES、PyMOL等分析平台,分析鉴定EXP基因家族成员,解析其基因表达模式。【结果】共筛选出23株四倍体油橄榄植株,得率为24.46%;四倍体与二倍体相比,植株出现巨大化的表型,表皮细胞和气孔极显著增大(P<0.0001);共筛选到4743个差异基因,其中2371个上调,2372个下调;通过差异基因分析,富集到与细胞巨大化密切相关的生长素信号转导通路,鉴定出2个显著上调的EXP成员,并进行qRT-PCR验证;在全基因组水平上完成了对EXP基因家族的分析。【结论】EXP基因家族与四倍体细胞巨大化密切相关,为今后进一步探索多倍体巨大化表型奠定了基础。
摘要In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to be special values, the solitary wave solutions are derived from the exact traveling wave solutions. The validity and reliability of the method are tested by its applications to Nano-ionic solitons wave’s propagation along microtubules in living cells and Nano-ionic currents of MTs which play an important role in biology.
摘要Objective To investigate the distribution of bra in-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, a lso in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulati on of RGCs.
摘要For the multi-parameter discrete exponential family,we construct an empirical Bayes(EB)estimator of the vector-valued parameterθ.under some conditions,this estimator is proved to be asymptotically optimal.
摘要This paper applies the EXP-function method to find exact solutions of various nonlinear equations. Tzitzeica- Dodd-Bullough (TDB) equation was selected to illustrate the effectiveness and convenience of the suggested method. More generalized solitonary solutions with free parameters were obtained by suitable choice of the free parameters, and also the obtained solitonary solutions can be converted into periodic solutions.