Camellia impressineruis(Jnhua tea),often called the'Queen of Camellia'or'Flora Panda',is a group of yellow-flowering camellias with notable ornamental,breeding,and medicinal value(Huang et al.,2022).De...Camellia impressineruis(Jnhua tea),often called the'Queen of Camellia'or'Flora Panda',is a group of yellow-flowering camellias with notable ornamental,breeding,and medicinal value(Huang et al.,2022).Despite this inherent value,the molecular regulatory mechanisms that produce its golden flower coloration and characteristic tea flavor remain poorly understood.Here,we present a high-quality,chromosomescale genome assembly of C.impressineruis to understand the molecular basis of yellow flower formation and flavor development,providing valuable scientific resources for future research on Jinhua tea.展开更多
Flowering time is a crucial agronomic trait that affects the adaptability and yield of soybeans.Despite extensive research aimed at uncovering the genetic basis of flowering time in soybean germplasm,investigations in...Flowering time is a crucial agronomic trait that affects the adaptability and yield of soybeans.Despite extensive research aimed at uncovering the genetic basis of flowering time in soybean germplasm,investigations involving the soybean germplasm from Northeast China have been limited.Here,we elucidated the genetic basis of days to flowering(DTF)in the soybean germplasm grown in Northeast China by employing an integrated strategy,including association mapping,quantitative trait locus(QTL)analysis,haplotype analysis,and candidate gene analysis.Overall,the genome-wide association study(GWAS)revealed 15 single-nucleotide polymorphisms(SNPs)significantly linked with DTF across six GWAS models and four individual environments plus a combined environment(CE).Five stable QTL were identified,among which four(viz.,qDTF8,qDTF12,qDTF15.1,and qDTF15.2)are reported for the first time,and the remaining one(qDTF19)was detected in previous studies.On the basis of the findings of expression and haplotype analysis,fourteen putative candidate genes were detected across the genomic intervals of these five QTL.Among these genes,Glyma.19 g197600 and Glyma.19 g200700 were confirmed through both expression and haplotype analysis.Moreover,the relationship of the haplotypes with gene expression indicates that Glyma.19 g197600 is a negative regulator of early flowering,thereby providing evidence of its role in flowering.This study reveals potential genetic loci and genes that could facilitate the expansion of soybean cultivation into regions with short growing seasons and long photoperiodic conditions across the globe.展开更多
Highlights·A TaPRR95-A haplotype reduces flowering time and increases grain yield in wheat.·Protein-protein interaction between TaPRR95-A and TaCOL16-D modulates the TaFT1-mediated flowering pathway.·A ...Highlights·A TaPRR95-A haplotype reduces flowering time and increases grain yield in wheat.·Protein-protein interaction between TaPRR95-A and TaCOL16-D modulates the TaFT1-mediated flowering pathway.·A validated functional marker enables rapid identification of the target haplotype and provides a practical tool for markerassisted selection in wheat breeding.As a staple cereal crop cultivated worldwide,common wheat(Triticum aestivum L.)accounts for approximately 35%of global dietary energy requirements(Sharma and Sharma 2025).However,agricultural yields are currently under significant pressure due to the combined impacts of anthropogenic climate change and geopolitical conflicts(Ortiz-Bobea et al.2021;Hultgren et al.2025),which threatens global food security.These challenges necessitate the urgent development of molecular breeding approaches to improve wheat production sustainably.展开更多
The Jumonji C domain-containing(JmjC)histone demethylases(JMJs)are involved in various aspects of plant development and responses to environmental changes.AtJMJs have been extensively studied in Arabidopsis for their ...The Jumonji C domain-containing(JmjC)histone demethylases(JMJs)are involved in various aspects of plant development and responses to environmental changes.AtJMJs have been extensively studied in Arabidopsis for their roles in regulating flowering time,while their functions and molecular mechanisms in regulating flowering time in rice remain underexplored.Here,we demonstrate that the JmjC domain-only group member OsJMJ712 regulates heading date in rice.OsJMJ712 exhibits H3K36me3 demethylase activity at Ehd1 and RFT1 and represses the expression of Ehd1,Hd3a,and RFT1.Furthermore,loss of function of OsJMJ712 disrupts the circadian clock,and OsLHY directly binds to the promoter of OsJMJ712 to suppress its expression.These findings uncover that OsJMJ712 integrates histone demethylation and the circadian clock to fine-tune photoperiodic flowering in rice,providing new insights into the epigenetic control of photoperiodic flowering in crops.展开更多
Salvia miltiorrhiza-derived carbon dots(SmCDs)have a potent antioxidant capacity.Squamosa promoter-binding protein-like(SPL)transcription factors respond to both heat and cold stress.However,the molecular mechanisms u...Salvia miltiorrhiza-derived carbon dots(SmCDs)have a potent antioxidant capacity.Squamosa promoter-binding protein-like(SPL)transcription factors respond to both heat and cold stress.However,the molecular mechanisms underlying the regulation of both high temperature(HT)and low temperature(LT)stress by SmCDs through SPL-mediated ascorbate(AsA)biosynthesis and recycling pathways remain unexplored.Therefore,we systematically identified 29 BrSPLs and 16 genes related to AsA biosynthesis and recycling pathways in flowering Chinese cabbage.BrSPL11.1 and three genes(BrGalDH,BrAPX,and BrDHAR1)involved in AsA biosynthesis and recycling pathways were induced by both HT and LT,and their expression patterns were modulated by SmCDs.Functional analyses revealed that the expression of BrGalDH,BrAPX,and BrDHAR1 was suppressed in BrSPL11.1 over expressing(BrSPL11.1-OE)plants,leading to reduced AsA content and reactive oxygen species(ROS)accumulation,as well as enhanced sensitivity to thermal extremes.Conversely,atspl11.1 showed inverse phenotypic and biochemical trends.BrSPL11.1 directly bound to the promoters of BrGalDH,BrAPX,or BrDHAR1 and suppressed their transcription.Silencing BrGalDH,BrAPX,or BrDHAR1 decreased the AsA content and increased ROS accumulation,decreasing HT and LT resistance.SmCDs application effectively elevated the AsA content and attenuated ROS accumulation,alleviating oxidative damage under both stress conditions.Our results established that BrSPL11.1 acts as a dual-temperature stress repressor and that SmCDs enhance HT and LT resistance by regulating BrSPL11.1-mediated AsA biosynthesis and recycling pathways.展开更多
Plants perceive rhythmic photoperiodic signals to modulate flowering time.In Arabidopsis thaliana,long-day light conditions accelerate flowering through CONSTANS(CO)-activated FLOWERING LOCUS T(FT)signal pathway.The C...Plants perceive rhythmic photoperiodic signals to modulate flowering time.In Arabidopsis thaliana,long-day light conditions accelerate flowering through CONSTANS(CO)-activated FLOWERING LOCUS T(FT)signal pathway.The CO protein abundance presents circadian oscillation,enabling precise regulation of FT transcription.NTL8 belongs to the NAC transcription factor family and is reported to control leaf trichome development.Here,we reported that NTL8 regulated flowering time in Arabidopsis,because overexpressing NTL8 significantly delayed flowering time,whereas loss-of-function mutant of NTL8 accelerated flowering time.NTL8 also presented circadian expression,maintaining elevated transcript levels during the daytime.Biochemical and genetic analyses revealed that NTL8 physically interacted with CO in planta,thereby antagonizing CO activity and repressing FT expression to delay the flowering time.Furthermore,overexpressing NTL8 reduced the protein stability of CO,particularly by attenuating CO accumulation in the morning time and promoting CO degradation during the night.Collectively,our findings indicated that NTL8 delayed photoperiod-dependent flowering time by suppressing CO-mediated activation of FT expression and destabilizing CO protein.This study uncovers a previously unrecognized role for NTL8 in coordinating circadian and photoperiodic signals to fine-tune flowering time in Arabidopsis thaliana.展开更多
Objective:To investigate the effects and potential mechanisms of action of Panax notoginseng(Burk)F.H.Chen(P.notoginseng,San Qi)flowers in type 2 diabetes mellitus(T2DM)using network pharmacology,in vivo experiments,a...Objective:To investigate the effects and potential mechanisms of action of Panax notoginseng(Burk)F.H.Chen(P.notoginseng,San Qi)flowers in type 2 diabetes mellitus(T2DM)using network pharmacology,in vivo experiments,and RNA sequencing(RNA-seq).Methods:Network pharmacology analysis was performed to identify and correlate the drug targets of flower buds of P.notoginseng(PNF)with T2DM disease targets and to predict the key targets and pathways involved in the therapeutic effects of PNF in T2DM.In vivo experiments were conducted to assess the effects of PNF on glucose and lipid metabolism in mice with T2DM.RNA-seq was performed,and the results were integrated with network pharmacology data to assess the therapeutic mechanisms of PNF in T2DM.The results from transcriptomics and network pharmacology were validated using real-time polymerase chain reaction.Results:A total of 27 intersecting targets were identified by overlapping 35 drug targets with T2DM targets.Further topological analysis using the Centiscape 2.2 tool revealed five core targets,including signal transducer and activator of transcription 3(STAT3).Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis indicated that the JAK/STAT signaling pathway is a key mechanism underlying the therapeutic effects of PNF in T2DM.In vivo experiments confirmed that PNF effectively regulates glycolipid metabolism in a mouse model of diabetes.KEGG pathway enrichment analysis of RNA-seq data highlighted the JAK2/STAT3 and PI3K/AKT pathway as a potential mechanism.PNF high-dose(PNFH)increased the gene expression levels of PIK3R1 and AKT2,decreased the expression of PCK1,JAK2,and STAT3,and showed a trend toward increasing INSR expression without reaching statistical significance.Conclusion:PNF improves glycolipid metabolism disorders in T2DM,potentially by modulating the JAK2/STAT3 and PI3K/AKT signaling pathway.展开更多
Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due t...Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due to extreme climatic conditions and facilitate the cultivation of subsequent crops on the same land,thereby enhancing overall agricultural efficiency.In this review,we synthesize current information on flowering time regulation in rapeseed through an integrated analysis of its genetic,hormonal,and environmental dimensions,emphasizing their crosstalk and implications for yield.We consolidate multi-omics evidence from population genetics,functional genomics,and systems biology to create a haplotype-based framework that overcomes the trade-off between flowering time and yield,providing support for the precision breeding of early-maturing cultivars.The insights presented here could inform future research on flowering time regulation and guide strategies for increasing rapeseed productivity.展开更多
An inappropriate flowering time and drought stress resistance in Brassica juncea(mustard)will decrease its yield and quality.B-box(BBX)transcription factors play an important role in the regulation of flowering and dr...An inappropriate flowering time and drought stress resistance in Brassica juncea(mustard)will decrease its yield and quality.B-box(BBX)transcription factors play an important role in the regulation of flowering and drought tolerance,but the biological functions and molecular mechanisms of the BBX family—especially BjuBBX6—remain largely unknown in Brassica juncea.In this study,we cloned the BjuBBX6-1 gene from Brassica juncea and found that it was expressed mainly in flowers and leaves.BjuBBX6-1 was localized in the nucleus and had transcriptional activation activity.Overexpressing BjuBBX6-1 caused earlier flowering compared with the wild type,while BjuBBX6-1 silencing in RNAi lines led to later flowering.BjuBBX6-1 interacted with flowering factors BjuNF-YB2 and BjuNF-YB3 and subsequently bound to the promoters of its downstream genes to promote BjuABI2 expression.However,it repressed BjuFLC and BjuGLK expression,resulting in earlier flowering.Under drought stress,overexpressing BjuBBX6-1 plants reduced chlorophyll content and antioxidant enzyme activities,and increased malondialdehyde(MDA)content,while silencing BjuBBX6-1 plants exhibited the opposite trend.The results indicate that BjuBBX6-1 negatively regulates drought tolerance in Brassica juncea.This study lays a theoretical foundation for in-depth research on the molecular mechanism of the BBX family in flowering regulation and drought tolerance breeding of Brassica juncea.展开更多
Almond[Prunus dulcis(Mill.)D.A.Webb]is one of the oldest cultivated nut tree crops in the world.Breeding efforts aim to develop new cultivars with improved economically significant traits,including higher yields and s...Almond[Prunus dulcis(Mill.)D.A.Webb]is one of the oldest cultivated nut tree crops in the world.Breeding efforts aim to develop new cultivars with improved economically significant traits,including higher yields and superior kernel quality,which have a complex genetic architecture.Almond yield has been linked to traits such as flowering or fruit set,among others.Despite the importance of flowering time,its genetic control is still not fully understood in almond trees.Recent advancements in genomics,including the new almond Axiom 60K SNP array and the availability of transcriptomic resources have enabled researchers to integrate various findings from QTL analyses,genome-wide association studies(GWAS)and gene expression and protein network interactions.This study examined 194 individuals,including 80 genotypes from the CEBAS-CSIC germplasm collection and an F1 progeny with 114 descendants from the cross between the French selection‘R1000’and the Spanish cultivar‘Desmayo Largueta’.We obtained an ultra-high-density genetic map with 4583 SNPs,with a density of 0.46 cM/marker and a total length of 509.29 cM.We identified 29 QTLs:10 for flowering time(FT)and 19 for blooming density(BD).The GWAS identified significant associations for 23 SNP traits using different models.A total of 35 SNPs exhibited identical allelic effect patterns in the F1 and the germplasm.Using SNPeff,we detected 104 distinct effects.Combined with re-analyzed RNA-seq data and protein–protein interaction analyses,the results allowed us to detect a list of candidate genes,including Prudul26A008435(L-ascorbate oxidase-like),Prudul26A008619(UDP-glucose 4-epimerase),and Prudul26A007993(gibberellin 2-β-dioxygenase),which are associated with flowering time as well as Prudul26A003733(Aminotransferase class IV),which is linked to blooming density.This information will contribute to a comprehensive understanding of flowering,a crucial almond trait.展开更多
Flowering is a necessary condition and basis for yield in the life cycle of woody fruit trees.Although there has been considerable interest in the regulatory mechanisms underlying floral induction and flowering,the as...Flowering is a necessary condition and basis for yield in the life cycle of woody fruit trees.Although there has been considerable interest in the regulatory mechanisms underlying floral induction and flowering,the associated epigenetic modifications remain poorly characterized.We identified genome-wide DNA methylation changes and the transcriptional responses in axillary buds of‘Qinguan'(QA)and‘Fuji'(FA)varieties with contrasting flowering behaviors.The DNA methylation levels were 19.35,62.96 and 17.68%in FA,and 19.64,62.49 and 17.86%in QA in the CG,CHG and CHH contexts,respectively.The number of hypermethylated and hypomethylated differentially methylated regions(DMRs)in different regions contributed to significantly up-and downregulated gene expression.DNA methylation can positively or negatively regulate gene expression depending on the CG,CHG and CHH contexts and their locations in different regions.Additionally,the huge differences in transcription of MIKCc-type MADS-box genes,and multiple fiowering genes in multiple flowering pathways(i.e.,light,aging,GA and sugar)by changing DNA methylation,contributed to contrasting flowering behaviors in both QA and FA.Specifically,the fioral meristem identity genes(i.e.,FT,LEAFY,AP1 and SOC1)exhibited significantly higher expression in QA than FA,but the fioral repressors(i.e.,SVP,AGL15,and AGL18)showed the opposite trend.Significant differences in multiple hormone levels were due to differentially expressed genes(DEGs)and their DMRs in hormone synthesis pathways,leading to both contrasting axillary bud outgrowth and fiowering behaviors.These findings refiect the diversity in the epigenetic regulation of gene expression and may be helpful for elucidating the epigenetic regulatory mechanism underlying the axillary bud fiowering in apple.展开更多
As a pivotal global oil crop,soybean production plays a vital role in ensuring food security and promoting sustainable development.The processes of flowering and pod drop are critical determinants of soybean yield,thu...As a pivotal global oil crop,soybean production plays a vital role in ensuring food security and promoting sustainable development.The processes of flowering and pod drop are critical determinants of soybean yield,thus,effective regulation of flowering is essential for achieving both high and stable yields.The differentiation of flower buds marks a crucial stage in the flowering process,governed by a complex interplay of environmental and endogenous pathways,including photoperiodic,vernalization,autonomous,gibberellin,and age pathways.These pathways converge to integrate flowering signals,subsequently activating downstream floral meristem identity genes that orchestrate the formation of floral primordia.Sucrose,the primary sugar transport form in soybeans,serves not only as a fundamental component of carbon metabolism but also as a significant signaling molecule.Through the age pathway,sucrose harmonizes various flowering-related genes,thereby influencing the timing of soybean flowering.Gibberellin,an essential hormone for plant growth and development,modulates flowering through the gibberellin pathway,with DELLA proteins acting as key regulators in the signal transduction cascade.The synergistic interaction between sucrose and gibberellin on gene expression occurs via distinct signaling pathways,collectively orchestrating flower bud differentiation.A thorough exploration of the molecular mechanisms by which sugars and hormones regulate flowering is anticipated to yield valuable insights and guidance for enhancing field crop production.展开更多
Cucumber(Cucumis sativus L.)is a major vegetable crop worldwide,and its yield and quality are closely linked to flower development.AGAMOUS-LIKE 6(AGL6),a member of the ancient MADS-box transcription factor family,play...Cucumber(Cucumis sativus L.)is a major vegetable crop worldwide,and its yield and quality are closely linked to flower development.AGAMOUS-LIKE 6(AGL6),a member of the ancient MADS-box transcription factor family,plays a crucial role in flower development.However,the specific functions of its homolog in cucumber remain poorly understood.In this study,we demonstrate that CsAGL6 is predominantly expressed in flowers,with high expression levels observed in all floral organ primordia during the early stages of floral development.The petals of Csagl6 mutants exhibit a greener color compared to wild-type plants,along with a significant increase in total chlorophyll content.Additionally,the mutants show abnormal petal morphology,including changes in size and shape,as well as enlarged sepals resembling leaves occasionally.Molecular analysis reveals that CAULIFLOWER(CAL)and the E-class gene SEPALLATA 4(SEP4)are significantly downregulated in the mutants,while the chlorophyll synthesis gene Early Light-Induced Protein 1(ELIP1)and several stress-related genes in the chloroplasts are dramatically upregulated.Our findings provide novel insights into the functional role of CsAGL6 in regulating sepal and petal development,and offer a potential avenue for understanding the genetic control of flower pigmentation and organ morphology in Cucumis species.展开更多
With the rapid integration of modern information technologies into agriculture,smart agriculture enables increasingly precise phenotyping and yield prediction.Flower counting is a key phenological indicator;however,ac...With the rapid integration of modern information technologies into agriculture,smart agriculture enables increasingly precise phenotyping and yield prediction.Flower counting is a key phenological indicator;however,achieving high precision remains a significant challenge due to severe occlusion,density variations,and environmental variability(e.g.,lighting/weather).Moreover,existing studies remain fragmented without a comprehensive synthesis.To bridge this fundamental research gap,we present the first systematic survey of computer-vision-based flower counting.In this work,we propose a novel taxonomy that categorizes methods into static(single-image)and dynamic(video/multi-view)paradigms and elucidates their evolutionary trajectory.Unlike conventional reviews,we conduct a multi-scale evaluation encompassing both horizontal(methodological evolution from traditional to deep learning)and vertical(cross-species and scene-condition)performance analyses.Crucially,we validate representative algorithms on deployed platforms—UAV and ground robots—through engineering case studies that quantify real-world trade-offs(e.g.,height-accuracy and latency-robustness).Finally,we discuss prevailing limitations and propose future directions,including graph-based reasoning and Agri Verse integration(i.e.,agriculture-centric metaverse or digital twin ecosystems),establishing a foundational framework for both academic research and industrial deployment.展开更多
This study investigated the association between floral and fruiting traits in oil tree peony to inform elite breeding and hybrid programmes.Floral and fruiting metrics of Paeonia ostii‘Fengdanbai’and Paeonia rockii...This study investigated the association between floral and fruiting traits in oil tree peony to inform elite breeding and hybrid programmes.Floral and fruiting metrics of Paeonia ostii‘Fengdanbai’and Paeonia rockii‘Zibanbai’were recorded under natural conditions;data were analysed using independent-samples t-tests,Pearson correlations,stepwise multiple linear regression and membership functions.Paeonia ostii‘Fengdanbai’produced more pollen number per flower,but fewer ovules and seeds per fruit than Paeonia rockii‘Zibanbai’(p<0.05).Pollen viability and stigma receptivity followed similar unimodal temporal curves in both taxa.Peak pollen viability occurred on day 3 after anthesis in P.ostii‘Fengdanbai’(86.95±1.41%)and on day 2 in P.rockii‘Zibanbai’(85.91±2.99%).Optimal stigma receptivity spanned days 3–5 and 2–5 post-anthesis,respectively,yielding a 3-day overlap favourable for synchronous pollination.Seed number per fruit correlated positively with petal number per flower(r=0.49),ovule number per fruit(r=0.68)and seed setting rate(r=0.70,p<0.01);carpel number also contributed(r=0.31,p<0.05).Stepwise multiple regression identified ovule number per fruit and seed setting rate as the most significant predictors of seed number per fruit(p<0.001),together explaining over 99%of the variation in the model(adjusted R2=0.992).Membership function analysis,based on pollen viability,ovule number per fruit,and seed setting rate,suggested that‘Fengdanbai’exhibits superior comprehensive performance,though selection indices should be validated across broader germplasm.We propose ovule number per fruit and seed setting rate as primary selection indices,with pollen viability as a supplementary indicator,for consideration in breeding high-yield cultivars.展开更多
Data serves as the foundation for training and testing machine learning and artificial intelligencemodels.The most fundamental part of data is its attributes or features.The feature set size changes from one dataset t...Data serves as the foundation for training and testing machine learning and artificial intelligencemodels.The most fundamental part of data is its attributes or features.The feature set size changes from one dataset to another.Only the relevant features contributemeaningfully to classificationaccuracy.The presence of irrelevant features reduces the system’s effectiveness.Classification performance often deteriorates on high-dimensional datasets due to the large search space.Thus,one of the significant obstacles affecting the performance of the learning process in the majority of machine learning and data mining techniques is the dimensionality of the datasets.Feature selection(FS)is an effective preprocessing step in classification tasks.The aim of applying FS is to exclude redundant and unrelated features while retaining the most informative ones to optimize classification capability and compress computational complexity.In this paper,a novel hybrid binary metaheuristic algorithm,termed hSC-FPA,is proposed by hybridizing the Flower Pollination Algorithm(FPA)and the Sine Cosine Algorithm(SCA).Hybridization controls the exploration capacity of SCA and the exploitation behavior of FPA to maintain a balanced search process.SCA guides the global search in the early iterations,while FPA’s local pollination refines promising solutions in later stages.A binary conversion mechanism using a threshold function is implemented to handle the discrete nature of the feature selection problem.The functionality of the proposed hSC-FPA is authenticated on fourteen standard datasets from the UCI repository using the K-Nearest Neighbors(K-NN)classifier.Experimental results are benchmarked against the standalone SCA and FPA algorithms.The hSC-FPA consistently achieves higher classification accuracy,selects a more compact feature subset,and demonstrates superior convergence behavior.These findings support the stability and outperformance of the hybrid feature selection method presented.展开更多
Anthocyanins(ACNs),a major class of water-soluble flavonoid pigments,are responsible for the vivid red,purple,and blue hues in many edible ornamental flowers.Recently,increasing attention has been directed toward thes...Anthocyanins(ACNs),a major class of water-soluble flavonoid pigments,are responsible for the vivid red,purple,and blue hues in many edible ornamental flowers.Recently,increasing attention has been directed toward these flowers not only for their aesthetic value but also for their nutritional and functional potential,such as antioxidant,anti-inflammatory,antidiabetic,anticancer,and cardioprotective activities.This review summarizes current knowledge on the source and biosynthesis pathways of ACNs in edible ornamental flowers,highlighting the key enzymes and regulatory genes involved.Factors affecting ACN stability,such as chemical structure,pH,temperature,light,oxygen,water activity,copigmentation,and microencapsulation are discussed in detail to provide insights into preserving their color and functionality in food systems.Eight extraction techniques,including solvent extraction(conventional method),and ultrasound-assisted,microwave-assisted,pulsed electric field-assisted,cold plasma-assisted and green solvent methods,are compared in terms of efficiency,sparing energy,environmental impact,and scalability.Among the 11 different types of edible flowers,the top four for total ACNs(g cyanindin-3-glucoside/kg DB)are rose(33.3),saffron(10.2),roselle(10.0),and butterfly pea flower(3.5),making them good sources of red to blue ACNs for exploring industrial scale-up.Finally,the review explores their diverse applications in the food industry as natural colorants,functional ingredients,and intelligent packaging films,offering potential for clean-label and health-oriented products.Overall,edible ornamental flowers represent a sustainable and multifunctional source of ACNs with promising applications in both nutrition and food technology.Future directions should focus on enhancing ACN stability,reducing extraction costs,and validating their efficacy through in vivo studies and industrial-scale trials.展开更多
The photoperiod is essential to flower induction,and the exact timing of the process can be precisely regulated based on the relative duration of light and darkness.However,the mechanisms linking photoperiod and flowe...The photoperiod is essential to flower induction,and the exact timing of the process can be precisely regulated based on the relative duration of light and darkness.However,the mechanisms linking photoperiod and flower induction in woody plants remain largely unexplored.Using RNA-seq,we identified a photoperiod response factor PmNAC32,which is predominantly expressed in early-flowering varieties.Overexpression of PmNAC32 in Arabidopsis thaliana,tobacco,and Prunus mume calli resulted in accelerated flowering.Binding and activation analyses revealed that PmNAC32 can be directly suppressed by REVEILLE 1(RVE1)and REVEILLE 3(RVE3),implying that PmNAC32 plays a role in the photoperiodic signaling pathway.Further studies established that PmNAC32 functions as a positive regulator of CONSTANS-LIKE 5(COL5)and a negative regulator of CONSTANS-LIKE 4(COL4).Interestingly,we identified two homologs of PmNAC32,namely PmNAC29 and PmNAC47.These three proteins can interact with each other and enhance the regulation of PmCOL4 and PmCOL5.Although PmNAC29 and PmNAC47 can promote flower induction respectively,neither of them responded to the photoperiod.Thus,our results reveal a novel mechanism by which PmNAC32 regulates flower induction in Prunus mume.展开更多
Carnation(Dianthus caryophyllus L.)is an important global flower crop,with great ornamental and economic value.It has more than 2000 years of cultivation history and profound cultural heritage known as mother flower.N...Carnation(Dianthus caryophyllus L.)is an important global flower crop,with great ornamental and economic value.It has more than 2000 years of cultivation history and profound cultural heritage known as mother flower.Now,although carnation is deeply loved by the majority of consumers because of its rich color and various varieties,the original carnation unique clove flavor has disappeared.Furthermore,our understanding of carnation traits such as flower shape,flower color,flower fragrance,disease resistance,and vase life remains limited.Previous reviews have primarily concentrated on individual aspects of carnation,failing to present a comprehensive overview.In this review,we summarize the recent progress of carnation in these aspects,so as to provide a reference for the future research direction in carnation.展开更多
基金supported by the Natural Science Foundation of Fujian Province(Grant No.2023J05216)Fujian Forestry Science and Technology Project(Grant No.2021FKJ24)。
摘要Camellia impressineruis(Jnhua tea),often called the'Queen of Camellia'or'Flora Panda',is a group of yellow-flowering camellias with notable ornamental,breeding,and medicinal value(Huang et al.,2022).Despite this inherent value,the molecular regulatory mechanisms that produce its golden flower coloration and characteristic tea flavor remain poorly understood.Here,we present a high-quality,chromosomescale genome assembly of C.impressineruis to understand the molecular basis of yellow flower formation and flavor development,providing valuable scientific resources for future research on Jinhua tea.
基金supported by the National Natural Science Foundation of China(U21A20215 and 32572440)the Biological Breeding-National Science and Technology Major Project(2023ZD0403201)+1 种基金the National Key Research and Development Program of China(2022YFD1500503)Science and Technology Development Plan Project of Jilin Province of China(20230101151JC)。
摘要Flowering time is a crucial agronomic trait that affects the adaptability and yield of soybeans.Despite extensive research aimed at uncovering the genetic basis of flowering time in soybean germplasm,investigations involving the soybean germplasm from Northeast China have been limited.Here,we elucidated the genetic basis of days to flowering(DTF)in the soybean germplasm grown in Northeast China by employing an integrated strategy,including association mapping,quantitative trait locus(QTL)analysis,haplotype analysis,and candidate gene analysis.Overall,the genome-wide association study(GWAS)revealed 15 single-nucleotide polymorphisms(SNPs)significantly linked with DTF across six GWAS models and four individual environments plus a combined environment(CE).Five stable QTL were identified,among which four(viz.,qDTF8,qDTF12,qDTF15.1,and qDTF15.2)are reported for the first time,and the remaining one(qDTF19)was detected in previous studies.On the basis of the findings of expression and haplotype analysis,fourteen putative candidate genes were detected across the genomic intervals of these five QTL.Among these genes,Glyma.19 g197600 and Glyma.19 g200700 were confirmed through both expression and haplotype analysis.Moreover,the relationship of the haplotypes with gene expression indicates that Glyma.19 g197600 is a negative regulator of early flowering,thereby providing evidence of its role in flowering.This study reveals potential genetic loci and genes that could facilitate the expansion of soybean cultivation into regions with short growing seasons and long photoperiodic conditions across the globe.
基金supported by the Key R&D Program of Shandong Province,China(2023LZGC022)the Shandong Provincial Natural Science Foundation,China(ZR2023QC103)the National Natural Science Foundation of China(U23A20181)。
摘要Highlights·A TaPRR95-A haplotype reduces flowering time and increases grain yield in wheat.·Protein-protein interaction between TaPRR95-A and TaCOL16-D modulates the TaFT1-mediated flowering pathway.·A validated functional marker enables rapid identification of the target haplotype and provides a practical tool for markerassisted selection in wheat breeding.As a staple cereal crop cultivated worldwide,common wheat(Triticum aestivum L.)accounts for approximately 35%of global dietary energy requirements(Sharma and Sharma 2025).However,agricultural yields are currently under significant pressure due to the combined impacts of anthropogenic climate change and geopolitical conflicts(Ortiz-Bobea et al.2021;Hultgren et al.2025),which threatens global food security.These challenges necessitate the urgent development of molecular breeding approaches to improve wheat production sustainably.
基金funded by the Zhejiang Zhengjingyuan Pharmacy Chain Co.,Ltd.,China(Grant No.2025-KYY-516030-0001).
摘要The Jumonji C domain-containing(JmjC)histone demethylases(JMJs)are involved in various aspects of plant development and responses to environmental changes.AtJMJs have been extensively studied in Arabidopsis for their roles in regulating flowering time,while their functions and molecular mechanisms in regulating flowering time in rice remain underexplored.Here,we demonstrate that the JmjC domain-only group member OsJMJ712 regulates heading date in rice.OsJMJ712 exhibits H3K36me3 demethylase activity at Ehd1 and RFT1 and represses the expression of Ehd1,Hd3a,and RFT1.Furthermore,loss of function of OsJMJ712 disrupts the circadian clock,and OsLHY directly binds to the promoter of OsJMJ712 to suppress its expression.These findings uncover that OsJMJ712 integrates histone demethylation and the circadian clock to fine-tune photoperiodic flowering in rice,providing new insights into the epigenetic control of photoperiodic flowering in crops.
基金financially supported by 2022—2025 Seed Industry Revitalization Project of Rural Revitalization Strategy Special Fund of Guangdong Province[Grant No.Yue Cai Nong(2022)No.184]Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams(Grant No.2023KJ122)+1 种基金Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515010505)Guangzhou Basic and Applied Basic Research Foundation(Grant No.2023A04J0114)。
摘要Salvia miltiorrhiza-derived carbon dots(SmCDs)have a potent antioxidant capacity.Squamosa promoter-binding protein-like(SPL)transcription factors respond to both heat and cold stress.However,the molecular mechanisms underlying the regulation of both high temperature(HT)and low temperature(LT)stress by SmCDs through SPL-mediated ascorbate(AsA)biosynthesis and recycling pathways remain unexplored.Therefore,we systematically identified 29 BrSPLs and 16 genes related to AsA biosynthesis and recycling pathways in flowering Chinese cabbage.BrSPL11.1 and three genes(BrGalDH,BrAPX,and BrDHAR1)involved in AsA biosynthesis and recycling pathways were induced by both HT and LT,and their expression patterns were modulated by SmCDs.Functional analyses revealed that the expression of BrGalDH,BrAPX,and BrDHAR1 was suppressed in BrSPL11.1 over expressing(BrSPL11.1-OE)plants,leading to reduced AsA content and reactive oxygen species(ROS)accumulation,as well as enhanced sensitivity to thermal extremes.Conversely,atspl11.1 showed inverse phenotypic and biochemical trends.BrSPL11.1 directly bound to the promoters of BrGalDH,BrAPX,or BrDHAR1 and suppressed their transcription.Silencing BrGalDH,BrAPX,or BrDHAR1 decreased the AsA content and increased ROS accumulation,decreasing HT and LT resistance.SmCDs application effectively elevated the AsA content and attenuated ROS accumulation,alleviating oxidative damage under both stress conditions.Our results established that BrSPL11.1 acts as a dual-temperature stress repressor and that SmCDs enhance HT and LT resistance by regulating BrSPL11.1-mediated AsA biosynthesis and recycling pathways.
基金supported by the National Natural Science Foundation of China(31970289).
摘要Plants perceive rhythmic photoperiodic signals to modulate flowering time.In Arabidopsis thaliana,long-day light conditions accelerate flowering through CONSTANS(CO)-activated FLOWERING LOCUS T(FT)signal pathway.The CO protein abundance presents circadian oscillation,enabling precise regulation of FT transcription.NTL8 belongs to the NAC transcription factor family and is reported to control leaf trichome development.Here,we reported that NTL8 regulated flowering time in Arabidopsis,because overexpressing NTL8 significantly delayed flowering time,whereas loss-of-function mutant of NTL8 accelerated flowering time.NTL8 also presented circadian expression,maintaining elevated transcript levels during the daytime.Biochemical and genetic analyses revealed that NTL8 physically interacted with CO in planta,thereby antagonizing CO activity and repressing FT expression to delay the flowering time.Furthermore,overexpressing NTL8 reduced the protein stability of CO,particularly by attenuating CO accumulation in the morning time and promoting CO degradation during the night.Collectively,our findings indicated that NTL8 delayed photoperiod-dependent flowering time by suppressing CO-mediated activation of FT expression and destabilizing CO protein.This study uncovers a previously unrecognized role for NTL8 in coordinating circadian and photoperiodic signals to fine-tune flowering time in Arabidopsis thaliana.
基金supported by the Creation and Talent Introduction Base of Prevention and Treatment of Diabetes and Its Complications withTraditional Chinese Medicine(B20055).
摘要Objective:To investigate the effects and potential mechanisms of action of Panax notoginseng(Burk)F.H.Chen(P.notoginseng,San Qi)flowers in type 2 diabetes mellitus(T2DM)using network pharmacology,in vivo experiments,and RNA sequencing(RNA-seq).Methods:Network pharmacology analysis was performed to identify and correlate the drug targets of flower buds of P.notoginseng(PNF)with T2DM disease targets and to predict the key targets and pathways involved in the therapeutic effects of PNF in T2DM.In vivo experiments were conducted to assess the effects of PNF on glucose and lipid metabolism in mice with T2DM.RNA-seq was performed,and the results were integrated with network pharmacology data to assess the therapeutic mechanisms of PNF in T2DM.The results from transcriptomics and network pharmacology were validated using real-time polymerase chain reaction.Results:A total of 27 intersecting targets were identified by overlapping 35 drug targets with T2DM targets.Further topological analysis using the Centiscape 2.2 tool revealed five core targets,including signal transducer and activator of transcription 3(STAT3).Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis indicated that the JAK/STAT signaling pathway is a key mechanism underlying the therapeutic effects of PNF in T2DM.In vivo experiments confirmed that PNF effectively regulates glycolipid metabolism in a mouse model of diabetes.KEGG pathway enrichment analysis of RNA-seq data highlighted the JAK2/STAT3 and PI3K/AKT pathway as a potential mechanism.PNF high-dose(PNFH)increased the gene expression levels of PIK3R1 and AKT2,decreased the expression of PCK1,JAK2,and STAT3,and showed a trend toward increasing INSR expression without reaching statistical significance.Conclusion:PNF improves glycolipid metabolism disorders in T2DM,potentially by modulating the JAK2/STAT3 and PI3K/AKT signaling pathway.
基金supported by the National Key Research and Development Program of China(2022YFD1200400)the National Natural Science Foundation of China(32272111)+4 种基金Special fund for youth team of the Southwest Universities(SWU-XJPY202306)Chongqing Natural Science Foundation(CSTB2024NSCQLZX0012)Modern Agro-industry Technology Research System(CARS-12)Chongqing Modern Agricultural Industry Technology System(COMAITS202504)Biological Breeding-National Science and Technology Major Project(2022ZD04008).We sincerely appreciate the Plant Editors team for English language editing of the manuscript,which significantly improved its clarity and overall quality.
摘要Flowering time is a critical agronomic trait with a profound effect on the productivity and adaptabillity of rapeseed(Brassica napus L.).Strategically advancing flowering time can reduce the risk of yield losses due to extreme climatic conditions and facilitate the cultivation of subsequent crops on the same land,thereby enhancing overall agricultural efficiency.In this review,we synthesize current information on flowering time regulation in rapeseed through an integrated analysis of its genetic,hormonal,and environmental dimensions,emphasizing their crosstalk and implications for yield.We consolidate multi-omics evidence from population genetics,functional genomics,and systems biology to create a haplotype-based framework that overcomes the trade-off between flowering time and yield,providing support for the precision breeding of early-maturing cultivars.The insights presented here could inform future research on flowering time regulation and guide strategies for increasing rapeseed productivity.
基金supported by the National Natural Science Foundation of China[Grant No.32172547]the Southwest University&Fuling Academy Joint Construction Project[Grant No.FLYJY202401]+1 种基金Key Project of Technological Innovation Application Development Plan of Chongqing[Grant No.CSTB2024TIAD-CYKJCXX0025]the Modern Pioneer District and County Project of Agricultural Science and Technology in Fulin of Chongqing(Modern Biotechnology Breeding of Mustard).
摘要An inappropriate flowering time and drought stress resistance in Brassica juncea(mustard)will decrease its yield and quality.B-box(BBX)transcription factors play an important role in the regulation of flowering and drought tolerance,but the biological functions and molecular mechanisms of the BBX family—especially BjuBBX6—remain largely unknown in Brassica juncea.In this study,we cloned the BjuBBX6-1 gene from Brassica juncea and found that it was expressed mainly in flowers and leaves.BjuBBX6-1 was localized in the nucleus and had transcriptional activation activity.Overexpressing BjuBBX6-1 caused earlier flowering compared with the wild type,while BjuBBX6-1 silencing in RNAi lines led to later flowering.BjuBBX6-1 interacted with flowering factors BjuNF-YB2 and BjuNF-YB3 and subsequently bound to the promoters of its downstream genes to promote BjuABI2 expression.However,it repressed BjuFLC and BjuGLK expression,resulting in earlier flowering.Under drought stress,overexpressing BjuBBX6-1 plants reduced chlorophyll content and antioxidant enzyme activities,and increased malondialdehyde(MDA)content,while silencing BjuBBX6-1 plants exhibited the opposite trend.The results indicate that BjuBBX6-1 negatively regulates drought tolerance in Brassica juncea.This study lays a theoretical foundation for in-depth research on the molecular mechanism of the BBX family in flowering regulation and drought tolerance breeding of Brassica juncea.
基金supported by grant PID2021-127421OB-I00 funded by MICIU/AEI/10.13039/501100011033“ERDF A way of making Europe” and formed part of the AGROALNEXT program+3 种基金supported by MCIN with funding from European Union NextGenerationEU (PRTR-C17.I1)by the Fundaci on S eneca with funding from Comunidad Aut onoma Regi on de Murcia (CARM)the Spanish MICIU for two predoc-toral grants (FPU20/00614 and FPU22/02784, respectively)the grant PRE2022-105038 funded by MICIU/AEI/10.13039/501100011033 and “ESF Investing in your future”
摘要Almond[Prunus dulcis(Mill.)D.A.Webb]is one of the oldest cultivated nut tree crops in the world.Breeding efforts aim to develop new cultivars with improved economically significant traits,including higher yields and superior kernel quality,which have a complex genetic architecture.Almond yield has been linked to traits such as flowering or fruit set,among others.Despite the importance of flowering time,its genetic control is still not fully understood in almond trees.Recent advancements in genomics,including the new almond Axiom 60K SNP array and the availability of transcriptomic resources have enabled researchers to integrate various findings from QTL analyses,genome-wide association studies(GWAS)and gene expression and protein network interactions.This study examined 194 individuals,including 80 genotypes from the CEBAS-CSIC germplasm collection and an F1 progeny with 114 descendants from the cross between the French selection‘R1000’and the Spanish cultivar‘Desmayo Largueta’.We obtained an ultra-high-density genetic map with 4583 SNPs,with a density of 0.46 cM/marker and a total length of 509.29 cM.We identified 29 QTLs:10 for flowering time(FT)and 19 for blooming density(BD).The GWAS identified significant associations for 23 SNP traits using different models.A total of 35 SNPs exhibited identical allelic effect patterns in the F1 and the germplasm.Using SNPeff,we detected 104 distinct effects.Combined with re-analyzed RNA-seq data and protein–protein interaction analyses,the results allowed us to detect a list of candidate genes,including Prudul26A008435(L-ascorbate oxidase-like),Prudul26A008619(UDP-glucose 4-epimerase),and Prudul26A007993(gibberellin 2-β-dioxygenase),which are associated with flowering time as well as Prudul26A003733(Aminotransferase class IV),which is linked to blooming density.This information will contribute to a comprehensive understanding of flowering,a crucial almond trait.
基金supported by the National Natural Science Foundation of China(32472668 and 32072522)。
摘要Flowering is a necessary condition and basis for yield in the life cycle of woody fruit trees.Although there has been considerable interest in the regulatory mechanisms underlying floral induction and flowering,the associated epigenetic modifications remain poorly characterized.We identified genome-wide DNA methylation changes and the transcriptional responses in axillary buds of‘Qinguan'(QA)and‘Fuji'(FA)varieties with contrasting flowering behaviors.The DNA methylation levels were 19.35,62.96 and 17.68%in FA,and 19.64,62.49 and 17.86%in QA in the CG,CHG and CHH contexts,respectively.The number of hypermethylated and hypomethylated differentially methylated regions(DMRs)in different regions contributed to significantly up-and downregulated gene expression.DNA methylation can positively or negatively regulate gene expression depending on the CG,CHG and CHH contexts and their locations in different regions.Additionally,the huge differences in transcription of MIKCc-type MADS-box genes,and multiple fiowering genes in multiple flowering pathways(i.e.,light,aging,GA and sugar)by changing DNA methylation,contributed to contrasting flowering behaviors in both QA and FA.Specifically,the fioral meristem identity genes(i.e.,FT,LEAFY,AP1 and SOC1)exhibited significantly higher expression in QA than FA,but the fioral repressors(i.e.,SVP,AGL15,and AGL18)showed the opposite trend.Significant differences in multiple hormone levels were due to differentially expressed genes(DEGs)and their DMRs in hormone synthesis pathways,leading to both contrasting axillary bud outgrowth and fiowering behaviors.These findings refiect the diversity in the epigenetic regulation of gene expression and may be helpful for elucidating the epigenetic regulatory mechanism underlying the axillary bud fiowering in apple.
基金supported by the earmarked fund for the Program on Industrial Technology System of National Soybean(CARS-04-PS21)。
摘要As a pivotal global oil crop,soybean production plays a vital role in ensuring food security and promoting sustainable development.The processes of flowering and pod drop are critical determinants of soybean yield,thus,effective regulation of flowering is essential for achieving both high and stable yields.The differentiation of flower buds marks a crucial stage in the flowering process,governed by a complex interplay of environmental and endogenous pathways,including photoperiodic,vernalization,autonomous,gibberellin,and age pathways.These pathways converge to integrate flowering signals,subsequently activating downstream floral meristem identity genes that orchestrate the formation of floral primordia.Sucrose,the primary sugar transport form in soybeans,serves not only as a fundamental component of carbon metabolism but also as a significant signaling molecule.Through the age pathway,sucrose harmonizes various flowering-related genes,thereby influencing the timing of soybean flowering.Gibberellin,an essential hormone for plant growth and development,modulates flowering through the gibberellin pathway,with DELLA proteins acting as key regulators in the signal transduction cascade.The synergistic interaction between sucrose and gibberellin on gene expression occurs via distinct signaling pathways,collectively orchestrating flower bud differentiation.A thorough exploration of the molecular mechanisms by which sugars and hormones regulate flowering is anticipated to yield valuable insights and guidance for enhancing field crop production.
基金was supported by funding from the National Natural Science Foundation of China(32222075 and 32472768)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2024-IVF)。
摘要Cucumber(Cucumis sativus L.)is a major vegetable crop worldwide,and its yield and quality are closely linked to flower development.AGAMOUS-LIKE 6(AGL6),a member of the ancient MADS-box transcription factor family,plays a crucial role in flower development.However,the specific functions of its homolog in cucumber remain poorly understood.In this study,we demonstrate that CsAGL6 is predominantly expressed in flowers,with high expression levels observed in all floral organ primordia during the early stages of floral development.The petals of Csagl6 mutants exhibit a greener color compared to wild-type plants,along with a significant increase in total chlorophyll content.Additionally,the mutants show abnormal petal morphology,including changes in size and shape,as well as enlarged sepals resembling leaves occasionally.Molecular analysis reveals that CAULIFLOWER(CAL)and the E-class gene SEPALLATA 4(SEP4)are significantly downregulated in the mutants,while the chlorophyll synthesis gene Early Light-Induced Protein 1(ELIP1)and several stress-related genes in the chloroplasts are dramatically upregulated.Our findings provide novel insights into the functional role of CsAGL6 in regulating sepal and petal development,and offer a potential avenue for understanding the genetic control of flower pigmentation and organ morphology in Cucumis species.
基金supported in part by the Foundation of Key Laboratory of Landscaping(KFL202402)the Ministry of Agriculture and Rural Affairs,Chinathe Research Start-Up Fund for Talent Introduction of Nanjing Agricultural University(77H0603)。
摘要With the rapid integration of modern information technologies into agriculture,smart agriculture enables increasingly precise phenotyping and yield prediction.Flower counting is a key phenological indicator;however,achieving high precision remains a significant challenge due to severe occlusion,density variations,and environmental variability(e.g.,lighting/weather).Moreover,existing studies remain fragmented without a comprehensive synthesis.To bridge this fundamental research gap,we present the first systematic survey of computer-vision-based flower counting.In this work,we propose a novel taxonomy that categorizes methods into static(single-image)and dynamic(video/multi-view)paradigms and elucidates their evolutionary trajectory.Unlike conventional reviews,we conduct a multi-scale evaluation encompassing both horizontal(methodological evolution from traditional to deep learning)and vertical(cross-species and scene-condition)performance analyses.Crucially,we validate representative algorithms on deployed platforms—UAV and ground robots—through engineering case studies that quantify real-world trade-offs(e.g.,height-accuracy and latency-robustness).Finally,we discuss prevailing limitations and propose future directions,including graph-based reasoning and Agri Verse integration(i.e.,agriculture-centric metaverse or digital twin ecosystems),establishing a foundational framework for both academic research and industrial deployment.
基金supported by the Innovation Scientists and Technicians Troop Construction Projects of Henan Province(212101510003).
摘要This study investigated the association between floral and fruiting traits in oil tree peony to inform elite breeding and hybrid programmes.Floral and fruiting metrics of Paeonia ostii‘Fengdanbai’and Paeonia rockii‘Zibanbai’were recorded under natural conditions;data were analysed using independent-samples t-tests,Pearson correlations,stepwise multiple linear regression and membership functions.Paeonia ostii‘Fengdanbai’produced more pollen number per flower,but fewer ovules and seeds per fruit than Paeonia rockii‘Zibanbai’(p<0.05).Pollen viability and stigma receptivity followed similar unimodal temporal curves in both taxa.Peak pollen viability occurred on day 3 after anthesis in P.ostii‘Fengdanbai’(86.95±1.41%)and on day 2 in P.rockii‘Zibanbai’(85.91±2.99%).Optimal stigma receptivity spanned days 3–5 and 2–5 post-anthesis,respectively,yielding a 3-day overlap favourable for synchronous pollination.Seed number per fruit correlated positively with petal number per flower(r=0.49),ovule number per fruit(r=0.68)and seed setting rate(r=0.70,p<0.01);carpel number also contributed(r=0.31,p<0.05).Stepwise multiple regression identified ovule number per fruit and seed setting rate as the most significant predictors of seed number per fruit(p<0.001),together explaining over 99%of the variation in the model(adjusted R2=0.992).Membership function analysis,based on pollen viability,ovule number per fruit,and seed setting rate,suggested that‘Fengdanbai’exhibits superior comprehensive performance,though selection indices should be validated across broader germplasm.We propose ovule number per fruit and seed setting rate as primary selection indices,with pollen viability as a supplementary indicator,for consideration in breeding high-yield cultivars.
基金supported by a research grant from Lahore College for Women University(LCWU),Lahore,Pakistan.
摘要Data serves as the foundation for training and testing machine learning and artificial intelligencemodels.The most fundamental part of data is its attributes or features.The feature set size changes from one dataset to another.Only the relevant features contributemeaningfully to classificationaccuracy.The presence of irrelevant features reduces the system’s effectiveness.Classification performance often deteriorates on high-dimensional datasets due to the large search space.Thus,one of the significant obstacles affecting the performance of the learning process in the majority of machine learning and data mining techniques is the dimensionality of the datasets.Feature selection(FS)is an effective preprocessing step in classification tasks.The aim of applying FS is to exclude redundant and unrelated features while retaining the most informative ones to optimize classification capability and compress computational complexity.In this paper,a novel hybrid binary metaheuristic algorithm,termed hSC-FPA,is proposed by hybridizing the Flower Pollination Algorithm(FPA)and the Sine Cosine Algorithm(SCA).Hybridization controls the exploration capacity of SCA and the exploitation behavior of FPA to maintain a balanced search process.SCA guides the global search in the early iterations,while FPA’s local pollination refines promising solutions in later stages.A binary conversion mechanism using a threshold function is implemented to handle the discrete nature of the feature selection problem.The functionality of the proposed hSC-FPA is authenticated on fourteen standard datasets from the UCI repository using the K-Nearest Neighbors(K-NN)classifier.Experimental results are benchmarked against the standalone SCA and FPA algorithms.The hSC-FPA consistently achieves higher classification accuracy,selects a more compact feature subset,and demonstrates superior convergence behavior.These findings support the stability and outperformance of the hybrid feature selection method presented.
基金funded by the Research Fund of Shanghai Sanda University(Project Numbers:2025YB12 and 2024BSZX02).
摘要Anthocyanins(ACNs),a major class of water-soluble flavonoid pigments,are responsible for the vivid red,purple,and blue hues in many edible ornamental flowers.Recently,increasing attention has been directed toward these flowers not only for their aesthetic value but also for their nutritional and functional potential,such as antioxidant,anti-inflammatory,antidiabetic,anticancer,and cardioprotective activities.This review summarizes current knowledge on the source and biosynthesis pathways of ACNs in edible ornamental flowers,highlighting the key enzymes and regulatory genes involved.Factors affecting ACN stability,such as chemical structure,pH,temperature,light,oxygen,water activity,copigmentation,and microencapsulation are discussed in detail to provide insights into preserving their color and functionality in food systems.Eight extraction techniques,including solvent extraction(conventional method),and ultrasound-assisted,microwave-assisted,pulsed electric field-assisted,cold plasma-assisted and green solvent methods,are compared in terms of efficiency,sparing energy,environmental impact,and scalability.Among the 11 different types of edible flowers,the top four for total ACNs(g cyanindin-3-glucoside/kg DB)are rose(33.3),saffron(10.2),roselle(10.0),and butterfly pea flower(3.5),making them good sources of red to blue ACNs for exploring industrial scale-up.Finally,the review explores their diverse applications in the food industry as natural colorants,functional ingredients,and intelligent packaging films,offering potential for clean-label and health-oriented products.Overall,edible ornamental flowers represent a sustainable and multifunctional source of ACNs with promising applications in both nutrition and food technology.Future directions should focus on enhancing ACN stability,reducing extraction costs,and validating their efficacy through in vivo studies and industrial-scale trials.
基金funded through the National Natural Science Foundation of China(32372670,31971703)the‘JBGS'Project of Seed Industry Revitalization in Jiangsu Province(BGS(2021]019)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Postgraduate Research and Practice Innovation Program of Jiangsu Province,China(KYCX23_0798).
摘要The photoperiod is essential to flower induction,and the exact timing of the process can be precisely regulated based on the relative duration of light and darkness.However,the mechanisms linking photoperiod and flower induction in woody plants remain largely unexplored.Using RNA-seq,we identified a photoperiod response factor PmNAC32,which is predominantly expressed in early-flowering varieties.Overexpression of PmNAC32 in Arabidopsis thaliana,tobacco,and Prunus mume calli resulted in accelerated flowering.Binding and activation analyses revealed that PmNAC32 can be directly suppressed by REVEILLE 1(RVE1)and REVEILLE 3(RVE3),implying that PmNAC32 plays a role in the photoperiodic signaling pathway.Further studies established that PmNAC32 functions as a positive regulator of CONSTANS-LIKE 5(COL5)and a negative regulator of CONSTANS-LIKE 4(COL4).Interestingly,we identified two homologs of PmNAC32,namely PmNAC29 and PmNAC47.These three proteins can interact with each other and enhance the regulation of PmCOL4 and PmCOL5.Although PmNAC29 and PmNAC47 can promote flower induction respectively,neither of them responded to the photoperiod.Thus,our results reveal a novel mechanism by which PmNAC32 regulates flower induction in Prunus mume.
基金supported by the National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops(Horti-3Y-2024-015)the Knowledge Innovation Program of Wuhan-Basic Research(Program No.2023020201010106)+4 种基金the Fundamental Research Funds for the Central Universities(Program No.2662023PY023 and 2662019PY049)the Thousand Youth Talents Plan Project and the Start-up Funding from Huazhong Agricultural University to FZby the Fundamental Research Funds for the Central Universities(Program No.2662023PY011)the Young Scientist Fostering Funds for the National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops(Program No.Horti-PY-2023-001)the Yunnan Xingdian Talents-Special Selection Project for High-level Scientific and Technological Talents and Innovation Teams-Team Specific Project(Program No.202505AS350021).
摘要Carnation(Dianthus caryophyllus L.)is an important global flower crop,with great ornamental and economic value.It has more than 2000 years of cultivation history and profound cultural heritage known as mother flower.Now,although carnation is deeply loved by the majority of consumers because of its rich color and various varieties,the original carnation unique clove flavor has disappeared.Furthermore,our understanding of carnation traits such as flower shape,flower color,flower fragrance,disease resistance,and vase life remains limited.Previous reviews have primarily concentrated on individual aspects of carnation,failing to present a comprehensive overview.In this review,we summarize the recent progress of carnation in these aspects,so as to provide a reference for the future research direction in carnation.