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Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation 认领 引用 被引量：1
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作者 Yinglong Song Wenqian Shang +7 位作者 Zheng Wang Songlin He Xinya Meng Liyun Shi Yuxiao Shen Dan He Xueyuan Lou Yuke Sun 《Phyton-International Journal of Experimental Botany》 SCIE 2022年第12期2791-2812,共22页
Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony.In this study,on the basis of transcriptome sequencing,differentially expressed genes and the associated metabolic pathw... Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony.In this study,on the basis of transcriptome sequencing,differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation.A total of 31.63 Gb raw data were generated and 120,188 unigenes(mean length of 911.98 bp)were annotated according to six databases(NR,NT,GO,KEGG,COG,and Swiss-Prot).Analyses of the ungerminated root primordium period,induced root primordium period,and root formation period detected 8,232,6,907,and 10,687 differentially expressed genes related to 133,132,and 133 metabolic pathways,respectively.Two significantly differentially expressed genes(Unigene13430_All and CL10096.Contig1_All)were associated with the auxin pathway.The full-length Unigene13430_All coding sequence(843 bp)encoded 280 amino acids,whereas the full-length CL10096.Contig1_All coding sequence(1,470 bp)encoded 489 amino acids.Unigene13430_All and CL10096.Contig1_All were identified as IAA gene family members and were respectively named PsIAA27 and PsARF19.The qRT-PCR analysis and functional verification indicated that the expressions of PsARF19 and PsIAA27 in tree peony seedlings,cuttings and grafted seedlings were significant different.PsARF19 promoted root development,it might be a regulatory gene related to the formation of tree peony roots,while PsIAA27 inhibited lateral root development,and it might be involved in controlling auxin sensitivity during root formation.The results of this study may form the basis of future investigations on the mechanism mediating peony root formation.The transcriptome data will be an excellent resource for researchers interested in characterizing the rooting-related tree peony genes. 展开更多
关键词 Tree peony rooting RNA-seq cuttage graft functional verification qRT-PCR
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Genome-wide identification of the JAZ gene family in non-heading Chinese cabbage and the functional verification of BcJAZ2 in the biosynthesis ofβ-caryophyllene 认领 引用 被引量：1
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作者 Tiantian Han Haibin Wang +6 位作者 Zhile Liang Aimei Bai Huanhuan Xu Jianjun Wang Tongkun Liu Xilin Hou Ying Li 《Vegetable Research》 2025年第1期430-441,共12页
JASMONATE ZIM DOMAIN(JAZ)proteins function as negative regulators of the JA signaling pathway and participate in plant development,stress responses,and secondary metabolism.β-caryophyllene is a volatile sesquiterpene... JASMONATE ZIM DOMAIN(JAZ)proteins function as negative regulators of the JA signaling pathway and participate in plant development,stress responses,and secondary metabolism.β-caryophyllene is a volatile sesquiterpene compound that contributes to the formation of plant aromas and possesses antibacterial,anti-inflammatory,and antifungal biological activities.In our previous experiments,we found that the BcJAZ2 was closely related toβ-caryophyllene synthesis under low-temperature treatment in non-heading Chinese cabbage(NHCC).To further explore the function of BcJAZ2,we characterized JAZ gene family in NHCC.In this study,25 BcJAZ genes were discovered in NHCC,and comprehensively analyzed the evolutionary relationships and structural characterizations of BcJAZs.BcMYC2,a positive regulator of terpenoid synthesis,interacted with BcJAZ2 confirmed by yeast two-hybrid and bimolecular fluorescence complementation assays.Overexpression of BcJAZ2 in Arabidopsis and silencing of BcJAZ2 in NHCC showed that BcJAZ2 acted as a negative regulator ofβ-caryophyllene biosynthesis.In addition,three transcription factors BcbHLH137,BcHBI1.1,and BcHBI1.2 were confirmed to be positive regulators of BcJAZ2 by yeast one-hybrid and LUC assays.The above results enrich our understanding of the regulation ofβ-caryophyllene synthesis and provide the foundation for in-depth exploration of regulatory mechanisms of BcJAZs. 展开更多
关键词 ja signaling pathway jaz gene family functional verification sesquiterpene compound genome wide identification bcjaz plant aromas non heading chinese cabbage
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Differing functions of two CYP716 genes for saikosaponin biosynthesis in Bupleurum chinense 认领 引用 被引量：1
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作者 Kaimi Dong Hua Chen +14 位作者 Ma Yu Shiying Wang Xue Yin Chuanxin Mo Meng Zhang Yuchan Li Yong Luo Zeru Yu Zisong Yang Jun Zhao Chao Xin Wenshuai Chen Zhen Ni Zheyong Xue Jianhe Wei 《The Crop Journal》 SCIE CSCD 2026年第1期224-234,共11页
Saikosaponins are the major pharmacologically active components in Bupleurum genus and exhibit significant application potential in multiple fields such as immune regulation and anti-tumor activity.To elucidate the bi... Saikosaponins are the major pharmacologically active components in Bupleurum genus and exhibit significant application potential in multiple fields such as immune regulation and anti-tumor activity.To elucidate the biosynthetic pathway of saikosaponins,we identified two cytochrome P450 monooxygenases,CYP716A41 and CYP716Y4,in Bupleurum chinense.These enzymes catalyze the C-28 oxidation and C-16 hydroxylation of oleanane-type triterpene skeletons,respectively.The catalytic efficiency of CYP716A41 from a southern B.chinense variety was significantly higher than that from a northern variety.Molecular docking and mutagenesis experiments revealed that amino acid residues at sites 9 and 35 may contribute to this difference in catalytic efficiency.Additionally,under cold stress,the expression levels of both CYP450 genes and the saikosaponin contents in the leaves of southern varieties were significantly higher compared to those in northern varieties.The variation in the catalytic efficiency of CYP716A41 and the differential expression of the two CYP450 genes under cold stress during winter are associated with the differences in saikosaponin biosynthesis in the leaves of southern and northern B.chinense varieties.This is consistent with the distinct medicinal usage practices observed between southern and northern China. 展开更多
关键词 Bupleurum chinense Medicinal tissue utilization Saikosaponin biosynthesis pathway CYP716 Functional verification
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Pigeon Robot for Navigation Guided by Remote Control:System Construction and Functional Verification 认领 引用 被引量：15
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作者 Zhengyue Zhou Denghui Liu +5 位作者 Hong Sun Wenbo Xu Xinmao Tian Xiaoyuan Li Han Cheng Zhenlong Wang 《Journal of Bionic Engineering》 SCIE EI CSCD 2021年第1期184-196,共13页
Animal robots have outstanding advantages over traditional robots in their own energy supplies,orientation,and natural concealment,delivering significant value in the theories and applications of neural science,nation... Animal robots have outstanding advantages over traditional robots in their own energy supplies,orientation,and natural concealment,delivering significant value in the theories and applications of neural science,national security,and other fields.Presently,many animal robots have been fabricated,but researches about the applications of avian robots are still lacking.In this study,we constructed a Pigeon Robot System(PRS),optimized the electric stimulation parameters,assessed the electric stimulus of navigation,and evaluated the navigation efficiency in the field.Biphasic pulse constant current pattern was adapted,and the optimal stimulus parameters of 4 nuclei tested were of amplitude 0.3 mA,5 pulse trains,frequency 25 Hz,5 pulses,and a 25%duty cycle.Effective ratio of left and right steering behavior response to electric stimulus dorsointermedius ventralis anterior nuclei was 67%and 83%,respectively(mean value 75%).Electrical stimulation efficiency was 0.34-0.68 and path efficiency was 0.72-0.85 among pigeon robot individuals in the open field.Neither electrical stimulation efficiency nor path efficiency differed significantly(P>0.05),suggesting that the navigational PRS performance was not biased in either direction.PRS can achieve continuous navigation along simple pathways and provide the necessary application infrastructure and technical reference for the development of animal robot navigation technology. 展开更多
关键词 pigeon robot bionic robot system construction navigation functional verification
Developing a homology-driven gene silencing system in Iris japonica:a versatile tool for comparative functional studies 认领 引用
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作者 Cheng Luo Zhiyan Wu +3 位作者 Lingmei Shao Jiaping Zhang Yiping Xia Danqing Li 《Ornamental Plant Research》 2025年第1期81-92,共12页
Iris japonica,an evergreen ornamental species known for its beautiful flowers and year-round evergreen foliage,is difficult to genetically manipulate due to the lack of an efficient genetic transformation system.This ... Iris japonica,an evergreen ornamental species known for its beautiful flowers and year-round evergreen foliage,is difficult to genetically manipulate due to the lack of an efficient genetic transformation system.This limitation hinders the functional verification of key genes in I.japonica.To address this,a Virus-Induced Gene Silencing(VIGS)system based on Tobacco rattle virus(TRV)was developed,enabling efficient,transient gene silencing in I.japonica.In this system,the phytoene desaturase(PDS)gene,a common reporter gene used for VIGS,was selected to assess the silencing efficiency.The recombinant vector pTRV2-IjPDS was used to infect I.japonica plants.The PDS gene silencing resulted in distinct photobleaching symptoms,demonstrating the successful silencing of the target gene.The presence of the TRV vector was verified by using a GFP-tagged pTRV2-GFP vector,and GFP expression was detected using fluorescence visualization under ultraviolet(UV)light and confirmed by laser confocal microscopy.Real-time PCR was used to quantify the reduction in IjPDS gene expression,confirming the successful silencing.The study further optimized the VIGS system by evaluating the impact of seedling ages on silencing efficiency,identifying one-year-old seedlings as the most effective for gene silencing(36.67%).This TRV-based VIGS system provides a robust tool for functional gene analysis in I.japonica and offers a new approach to studying the rules of key genes in its biological processes,with potential applications in ornamental plant breeding and genomics research. 展开更多
关键词 iris japonicaan tobacco rattle virus phytoene desaturase pds genea gene silencing genetic transformation systemthis functional verification key genes tobacco rattle virus trv virus induced gene silencing
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Functional analysis of the nitrogen metabolism-related gene CsGS1 in cucumber 认领 引用 被引量：1
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作者 XIN Ming QIN Zhi-wei +2 位作者 YANG Jing ZHOU Xiu-yan WANG Lei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第6期1515-1524,共10页
Glutamine synthetase(GS)plays an important role in nitrogen(N)metabolism in cucumber.In this study,we cloned and sequenced the CsGS1 gene,and analyzed the expression patterns and subcellular localization of the GS1 pr... Glutamine synthetase(GS)plays an important role in nitrogen(N)metabolism in cucumber.In this study,we cloned and sequenced the CsGS1 gene,and analyzed the expression patterns and subcellular localization of the GS1 protein in response to different N conditions in order to determine its role in low-nitrogen(LN)tolerance.CsGS1 was abundantly expressed in the leaves of the low N-requiring cultivar D0328,while the high N-requiring cultivar D0422 showed similar expression levels across different tissues including leaves,shoots and roots.Furthermore,the GS1 protein was primarily localized in the cytoplasm of plant cells.Both cultivars were then transformed with the CsGS1 coding sequence or antisense sequence via Agrobacterium tumefaciens in order to overexpress and silence GS1 expression,respectively.Overexpression of CsGS1 significantly improved LN tolerance and photosynthetic parameters,and increased chlorophyll b content,biomass,plant height,root length,N accumulation and GS activity under LN condition compared to the control.CsGS1 silencing on the other hand significantly reduced the above indices.Taken together,CsGS1 is crucial for maintaining N metabolism in cucumber plants during N deprivation,and is a promising target for generating novel transgenic breeds with increasing nitrogen utilization efficiency. 展开更多
关键词 cucumber CsGS1 low nitrogen functional verification
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Safety Design and Verification Analysis in the Satellite Development Stage 认领 引用
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作者 REN Xiangwen CHEN Baifeng 《Aerospace China》 2021年第4期21-27,共7页
A safety design applies to every stage in a satellite system development life cycle to identify and analyze hazards in the satellite at a system level, eliminating or controlling various safety risks, while verifying ... A safety design applies to every stage in a satellite system development life cycle to identify and analyze hazards in the satellite at a system level, eliminating or controlling various safety risks, while verifying the functions of the satellite system have safety characteristics, so as to optimize the satellite system for the best performance in terms of time and cost. This article comprehensively leverages such factors as satellite reliability, complexity and life cycle by considering the overall satellite safety work plan, hazard analysis, hazard sources, pyrotechnic devices and other module safety critical designs. Safety design measures were formulated to review and verify the effectiveness of system functions including a safe power supply to a satellite and pyrotechnic explosives to achieve the safety requirements of the satellite from a development stage. Safety design activities for each subsystem will ensure meeting the development requirements of the satellite system as a whole, and ensure the satellite system cannot be the cause of casualties, equipment damage, property loss, or have a health-threatening impact or detrimental impact on the environment. 展开更多
关键词 satellite system development project safety design functional verification
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The miR164a targets CsNAC1 to negatively regulate the cold tolerance of tea plants(Camellia sinensis) 认领 引用 被引量：1
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作者 Siya Li Lu Cao +3 位作者 Ziwen Zhou Yaohua Cheng Xianchen Zhang Yeyun Li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2025年第8期3073-3086,共14页
Cold stress widely impairs the quality and yield of tea plants. The miR164 family and its target NAC transcription factor have been identified as crucial regulators in response to cold stress. However, the role of miR... Cold stress widely impairs the quality and yield of tea plants. The miR164 family and its target NAC transcription factor have been identified as crucial regulators in response to cold stress. However, the role of miR164 and CsNAC in cold tolerance in tea plants was little understood. In our study, the expression level of CsMIR164a was significantly reduced under cold stress and significantly and negatively correlated with that of CsNAC1.5' RACE and GUS histochemical assays showed that CsNAC1 was cleaved by CsMIR164a. The CsMIR164a-silenced tea leaves promoted the expression levels of CsNAC1 and CsCBFs and exhibited greater cold tolerance. Also, the overexpression of CsNAC1 enhanced cold tolerance in transgenic Arabidopsis plants by promoting the expression levels of AtCBFs. In contrast, the heterologous overexpression of CsMIR164a in Arabidopsis decreased the expression level of AtNACs and AtCBFs and thus impaired cold tolerance. Additionally, silencing of CsNAC1 impaired the expression levels of CsCBFs, resulting in greater cold sensitivity in tea leaves. Our present study demonstrated that the miR164a-CsNAC1 module may play a negative role in the cold tolerance of tea plants via the CsCBF-dependent pathway. 展开更多
关键词 tea plants cold stress miRNAs NAC transcription factor functional verification
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Optimization of Callus Induction Conditions from Immature Embryos in Maize and Plant Regeneration 认领 引用 被引量：4
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作者 Peng Jiao Ruiqi Ma +5 位作者 Zhuo Qi Zhenzhong Jiang Siyan Liu Jing Qu Shuyan Guan Yiyong Ma 《Phyton-International Journal of Experimental Botany》 SCIE 2020年第1期121-130,共10页
This research uses the immature embryos of inbred maize lines(GSH9901,Hi01,Hi02,and Chang 7-2)as receptor materials to establish the callus induction system.These inbred lines provide the receptor materials for the ge... This research uses the immature embryos of inbred maize lines(GSH9901,Hi01,Hi02,and Chang 7-2)as receptor materials to establish the callus induction system.These inbred lines provide the receptor materials for the genetic regeneration of maize and the verification of the genetic functions of maize.The factor experiment and orthogonal experiments were used to investigate the impacts of different genotypes,immature embryo size,shield orientation,2,4-D concentration,proline concentration,and folic acid concentration on the induction rate of embryogenic callus tissue.A sensitivity experiment testing glyphosate(Bar)and an antibiotic(Cefotaxime sodium)were also conducted.The results indicate that the immature embryos of inbred maize line GSH9901 were the most effective for callus tissue induction,and the immature embryos with a length of 1.6-2.0 mm produce the best result.The upward shield face is more successful for the formation of induced callus.Using orthogonal analysis,we found that the optimal combination for the induction system was A3(2,4-D concentration 0.25 mg mL-1),B1C3(proline concentration 0.8 mg mL-1),and D 2(folate Concentration 0.5 mg mL-1)and the induction rate reached 84%.We found that cold storage at 4℃ for 1 d is more conducive for the formation of embryogenic callus than the other treatments tested.The sensitivity experiment for callus tissue screening revealed the critical concentration of glyphosate to be 10 mg ml-1,and the critical concentration of antibiotic is 250 mg ml-1.Using this combination of glyphosate and antibiotic resulted in regenerated plants.This study established the optimal conditions for immature embryo callus tissue induction in maize. 展开更多
关键词 Maize optimization genetic regeneration immature embryos function verification regenerated plants
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Genome-wide association studies reveal the genetic basis of amino acid content variation in tea plants 认领 引用 被引量：6
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作者 GUO Ya-fei LI Dai-li +4 位作者 QIU Hai-ji ZHANG Xiao-liang LIU Lin ZHAO Jing-jing JIANG De-yuan 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第11期3364-3379,共16页
Tea is one of the most popular non-alcoholic beverages in the world,and free amino acids,especially theanine,make a major contribution to the umami taste of tea.However,the genetic basis of the variation in amino acid... Tea is one of the most popular non-alcoholic beverages in the world,and free amino acids,especially theanine,make a major contribution to the umami taste of tea.However,the genetic basis of the variation in amino acid content in tea plants remains largely unknown.Here,we measured the free amino acid content in fresh leaves of 174 tea accessions over two years using a targeted metabolomics approach and obtained genotype data via RNA sequencing.Genome-wide association studies were conducted to investigate loci affecting the content of free amino acids.A total of 69 quantitative trait loci(–log10(P-value)>5)were identified.Functional annotation revealed that branched-chain amino acid aminotransferase,glutamine synthetase,nitrate transporter,and glutamate decarboxylase might be important for amino acid metabolism.Two significant loci,glutamine synthetase(Glu1,P=3.71×10−4;Arg1,P=4.61×10−5)and branched-chain amino acid aminotransferase(Val1,P=4.67×10−5;I_Leu1,P=3.56×10−6),were identified,respectively.Based on the genotyping result,two alleles of CsGS(CsGS-L and CsGS-H)and CsBCAT(CsBCAT-L and CsBCAT-H)were selected to perform function verification.Overexpression of CsGS-L and CsGS-H enhanced the contents of glutamate and arginine in transgenic plants,and overexpression of CsBCAT-L and CsBCAT-H promoted the accumulation of valine,isoleucine and leucine.Enzyme activity assay uncovered that SNP1054 is important for CsGS catalyzing glutamate into glutamine.Furthermore,CsGS-L and CsGS-H differentially regulated the accumulation of glutamine,and CsBCAT-L and CsBCAT-H differentially regulated the accumulation of branched-chain amino acids.In summary,the findings in our study would provide new insights into the genetic basis of amino acids contents variation in tea plants and facilitate the identification of elite genes to enhance amino acids content. 展开更多
关键词 Camellia sinensis amino acids genetic variation association studies genotype analysis functional verification
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Functional Verification for Agile Processor Development: A Case for Workflow Integration 认领 引用 被引量：1
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作者 徐易难 余子濠 +10 位作者 王凯帆 王华强 蔺嘉炜 金越 张林隽 张紫飞 唐丹 王卅 石侃 孙凝晖 包云岗 《Journal of Computer Science & Technology》 SCIE EI CSCD 2023年第4期737-753,共17页
Agile hardware development methodology has been widely adopted over the past decade.Despite the research progress,the industry still doubts its applicability,especially for the functional verification of complicated p... Agile hardware development methodology has been widely adopted over the past decade.Despite the research progress,the industry still doubts its applicability,especially for the functional verification of complicated processor chips.Functional verification commonly employs a simulation-based method of co-simulating the design under test with a reference model and checking the consistency of their outcomes given the same input stimuli.We observe limited collaboration and information exchange through the design and verification processes,dramatically leading to inefficiencies when applying the conventional functional verification workflow to agile development.In this paper,we propose workflow integration with collaborative task delegation and dynamic information exchange as the design principles to effectively address the challenges on functional verification under the agile development model.Based on workflow integration,we enhance the functional verification workflows with a series of novel methodologies and toolchains.The diff-rule based agile verification methodology(DRAV)reduces the overhead of building reference models with runtime execution information from designs under test.We present the RISC-V implementation for DRAV,DiffTest,which adopts information probes to extract internal design behaviors for co-simulation and debugging.It further integrates two plugins,namely XFUZZ for effective test generation guided by design coverage metrics and LightSSS for efficient fault analysis triggered by co-simulation mismatches.We present the integrated workflows for agile hardware development and demonstrate their effectiveness in designing and verifying RISC-V processors with 33 functional bugs found in NutShell.We also illustrate the efficiency of the proposed toolchains with a case study on a functional bug in the L2 cache of XiangShan. 展开更多
关键词 functional verification agile development open-source hardware workflow integration
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Automatic Circuit Extractor for HDL Description Using Program Slicing 认领 引用 被引量：1
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作者 TunLi YangGuo Si-KunLi 《Journal of Computer Science & Technology》 SCIE EI 2004年第5期718-728,共11页
Design extraction and reduction have been extensively used in modern VLSI design process. The extracted and reduced design can be efficiently processed by various applications, such as formal verification, simulation,... Design extraction and reduction have been extensively used in modern VLSI design process. The extracted and reduced design can be efficiently processed by various applications, such as formal verification, simulation, automatic test pattern generation (ATPG), etc. This paper presents a new circuit extraction method using program slicing technique, and develops an elegant theoretical basis based on program slicing for circuit extraction from Verilog description. The technique can obtain a chaining slice for given signals of interest. Compared with related researches, the main advantages of the method include that it is fine grain, it has no hardware description language (HDL) coding style limitation; it is precise and is capable of dealing with various Verilog constructions. The technique has been integrated with a commercial simulation environment and incorporated into a design process. The results of practical designs show the significant benefits of the approach. 展开更多
关键词 program slicing chaining slice process dependence graph circuit extraction VLSI functional verification
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