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Actionable Genotypes and Their Association with Life Span in Iceland 认领 引用
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作者 Brynjar O Jensson 《四川生理科学杂志》 2026年第3期551-551,共1页
Background:In 2021,the American College of Medical Genetics and Genomics(ACMG)recommended reporting actionable genotypes in 73 genes associated with diseases for which preventive or therapeutic measures are available.... Background:In 2021,the American College of Medical Genetics and Genomics(ACMG)recommended reporting actionable genotypes in 73 genes associated with diseases for which preventive or therapeutic measures are available.Evaluations of the association of actionable genotypes in these genes with life span are currently lacking. 展开更多
关键词 iceland american college medical genetics genomics life span preventive measures actionable genotypes preventive therapeutic measures therapeutic measures
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Comparative analysis between genotypes of adenovirus isolates from hospitalized children with acute respiratory tract infections and clinical manifestations in Wuhan,China,from June 2022 to September 2023 认领 引用 被引量：3
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作者 Chunchen Wu Yanfang Zhang +9 位作者 Ao Liang Xiaoxue Wu Yaqi Zhu Zhaoxuan Huang Jun Wang Yali Deng Lixian Pan Anbang Wang Fei Deng Jianbo Xia 《Virologica Sinica》 SCIE CAS CSCD 2025年第1期50-60,共11页
Acute respiratory tract infections(ARTIs)are among the leading causes of morbidity and mortality in children worldwide.Human adenovirus(HAdV)infections are estimated to account for at least 5%of pediatric ARTIs.The ci... Acute respiratory tract infections(ARTIs)are among the leading causes of morbidity and mortality in children worldwide.Human adenovirus(HAdV)infections are estimated to account for at least 5%of pediatric ARTIs.The circulated genotypes of HAdV and the correlation between genotype and clinical manifestations in Wuhan,China,before and after the complete relaxation of nonpharmaceutical interventions against severe acute respiratory syndrome coronavirus 2,remain unknown.Here,101 HAdV strains were isolated from throat swab samples collected from hospitalized children with ARTIs who tested positive for HAdV nucleic acid.Of these,sixty-six strains from 2022 to twenty-three strains from 2023 were successfully genotyped and subjected to phylogenetic analysis based on the hexon,penton base,and fiber genes.Six genotypes,B3,C1,C2,C5,C104,and C108 were identified.HAdV-B3(84.85%)was the most prevalent type in 2022,while HAdV-C(86.96%),including C1,C2,C108,and C104,was the most prevalent in 2023.These strains were phylogenetically related to strains from Japan,China,and the United States in recent years.When comparing clinical characteristics,pediatric patients infected with B3,C1,C2,C5,C104,or C108 exhibited similar clinical manifestations,primarily fever and cough,but varying interleukin(IL)-10 levels.In conclusion,from June 2022 to September 2023,the circulated genotypes of HAdV in Wuhan included B3,C1,C2,C108,C5,and C104.The endemic pattern of HAdV in Wuhan,China,shifted from species B as the dominant type in 2022 to species C in 2023. 展开更多
关键词 Acute respiratory tract infection (ARTIs) Human adenovirus(HAdV) Viral isolates Genotypes Clinical characteristics Pneumonia
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Genetic diversity assessment,germplasm resource conservation,and utilization in Elymus sibiricus from the margin of Northeast Qinghai-Tibet Plateau based on phenotypes and genotypes 认领 引用
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作者 Yuying Zheng Huanhuan Lu +2 位作者 Liuban Tang Fan Zhang Wengang Xie 《Grass Research》 2025年第1期231-241,共11页
Elymus sibiricus,a dominant native grass species on the margin of the Northeast Qinghai-Tibet Plateau(NEQTP)alpine meadows,is crucial for forage production and ecological restoration.However,limited studies on phenoty... Elymus sibiricus,a dominant native grass species on the margin of the Northeast Qinghai-Tibet Plateau(NEQTP)alpine meadows,is crucial for forage production and ecological restoration.However,limited studies on phenotypic and genetic diversity have hampered the protection and utilization of germplasm in E.sibiricus.In this study,the dual diversity evaluation based on phenotypes and genotypes was performed on 200 germplasms of 20 E.sibiricus populations using 19 phenotypic traits and 14 molecular markers.An abundant polymorphism was observed,with the phenotypic variation coefficient ranging from 2.97%(booting time)to 55.13%(breaking tensile strength)and Nei's genetic diversity index varying from 0.02(TZ03)to 0.17(LQ05).Both phenotype differentiation coefficient and molecular analysis of variance showed variation mainly from among populations.Clustering and STRUCTURE results showed that all populations are divided into two groups.A total of 24 loci were determined with a significant relationship to phenotypic traits by Tassel analysis,and the marker Ltc0121 can be used to identify both yield and flowering traits.Altitude distance(r=0.613,p=0.010)affected genetic diversity rather than geographical distance(r=0.044,p=0.220)for E.sibiricus.Our study evaluated the germplasm of Tianzhu County for the first time.According to the results of dual diversity evaluation,the lowest genetic diversity population TZ03 should be conserved using both in situ and ex situ approaches.The collection and evaluation of E.sibiricus in this study provided a key prerequisite for conservation and utilization in the margin of NEQTP. 展开更多
关键词 protection utilization germplasm phenotypes genotypes germplasms grass species molecular markersan dual diversity evaluation elymus sibiricusa forage production
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Evaluation of salt stress tolerance of three apple genotypes rootstock combinations in'Luli'apple 认领 引用
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作者 Guo-Lin Chen Da-Ru Wang +9 位作者 Xin Liu Xun Wang Lun Li Man-Shu Qu Jia-Yi Liu Hao-Feng Liu Hai-Bo Wang Sen Wang Lin-Guang Li Chun-Xiang You 《Fruit Research》 2025年第1期68-81,共14页
In rootstock-scion combinations,the rootstock is the key factor determining a plant's salt tolerance,primarily through its root physiology and soil interaction capabilities.However,the interaction between rootstoc... In rootstock-scion combinations,the rootstock is the key factor determining a plant's salt tolerance,primarily through its root physiology and soil interaction capabilities.However,the interaction between rootstock and scion in influencing salt tolerance in apple trees remains underexplored.In this study,three one-year-old dwarf rootstocks—'Qingzhen-1','M9-T337',and'(Malus hupehensis Rehd,var.Pingyiensis,PYTC)'—were selected,with'Luli'used as the scion.The salt tolerance was compared in natural soil(1‰),and saline-alkali soil from Dongying(3‰,6‰,10‰).The results showed that salt stress inhibited plant growth and damaged root structure.Among the combinations,Luli/Qingzhen-1 exhibited superior growth performance,with reduced malondialdehyde content,relative conductivity,and mortality.Additionally,in the Luli/Qingzhen-1 combination,genes associated with antioxidant enzyme activity and stress signal transduction were significantly upregulated.In conclusion,Qingzhen-1 demonstrates greater salt tolerance than M9-T337 and PYTC,making it a promising rootstock for cultivating'Luli'scions in saline-alkali soils. 展开更多
关键词 var Pingyiensis apple genotypes Malus hupehensis Rehd rootstock scion combinations salt tolerance salt stress tolerance Qingzhen
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Competitive outcomes of quinoa genotypes with wild radish and ryegrass at increasing levels of infestation 认领 引用
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作者 Leandro Galon Leticia Bampi +7 位作者 Daniel Cristian Cavaletti Leonardo Brunetto Germani Concenço Janete Denardi Munareto Eduardo Henrique Santin Cechet Alfredo Castamann Maico Andre Michelon Bagnara Gismael Francisco Perin 《Technology in Agronomy》 2025年第1期35-45,共11页
The weed species wild radish(Raphanus raphanistrum)and ryegrass(Lolium multiflorum)exhibit high competitive ability for resources such as water,light,and nutrients when infesting quinoa crops.The present study aimed t... The weed species wild radish(Raphanus raphanistrum)and ryegrass(Lolium multiflorum)exhibit high competitive ability for resources such as water,light,and nutrients when infesting quinoa crops.The present study aimed to compare the competitive ability of three quinoa genotypes(Q 1303,Q 1331,and Q 1324)in the presence of wild radish and ryegrass.The experiments were conducted under greenhouse conditions using a randomized complete block design with four replicates.Initially,the optimal plant density for quinoa genotypes and competing weeds was determined based on the point at which shoot dry matter production stabilized(20 plants per pot).Subsequently,five replacement series experiments were established to assess the competitive interactions between quinoa and the weeds,combining species in relative proportions of 100:0,75:25,50:50,25:75,and 0:100,corresponding to 20:0,15:5,10:10,5:15,and 0:20 plants per pot,respectively.Competitiveness was analyzed using replacement series diagrams and relative competition indices.Variables evaluated included plant height,stem diameter,number of leavesillers,chlorophyll index,leaf area,shoot dry matter,and nutrient content in both quinoa and competing plants.The quinoa genotypes competed for the same environmental resources as the weeds and showed similar competitive ability against each weed species.Overall,quinoa exhibited greater relative growth in the presence of ryegrass and reduced growth when infested with wild radish,which proved to be a more competitive weed species. 展开更多
关键词 compare competitive ability randomized complete block design wild radish ryegrass genotypes weed species competitive ability quinoa
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Transcriptomic Regulation Analysis Reveals Salt-Induced Response at Early Seedling Stages among Three Rice Genotypes 认领 引用
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作者 Sirinthorn KONGPRAPHRUT LÜ Yang +4 位作者 WANG Yueying Mohamed HAZMAN TONG Hanhua QIAN Qian GUO Longbiao 《Rice science》 SCIE CSCD 2025年第5期607-611,I0032-I0065,共5页
Salt stress is a critical factor affecting the growth and yield of rice.Egypt,the largest rice producer in North Africa and the Middle East,is facing contrasting challenges related to salinity in its agroecosystems.In... Salt stress is a critical factor affecting the growth and yield of rice.Egypt,the largest rice producer in North Africa and the Middle East,is facing contrasting challenges related to salinity in its agroecosystems.In this study,we compared the salt-induced responses among three rice varieties:Giza 176(Egyptian variety),Kaituodao(Chinese variety),and Pokkali(Sri Lanka variety,used as control)under normal and saline stress(100 mmol/L NaCl)conditions at two seedling stages through RNA-seq expression analysis.Giza 176 displayed a salt tolerance score of 7 on the SES(Standard Evaluation Score).Its transcriptome showed dynamic changes,with the number of upregulated genes increasing from 180 to 735,and downregulated genes increasing from 918 to 2930 from the one-week to two-week stress stages,with activated pathways in ion transport,reactive oxygen species(ROS)scavenging,and protein biosynthesis. 展开更多
关键词 protein biosynthesis ion transport reactive oxygen species salt stress seedling stages transcriptomic regulation rice genotypes RNA seq
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The differences non-volatile compound and flavor attributes in several Indonesian cocoa genotypes using medium scale fermentation 认领 引用
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作者 Indah Anita Sari Rudi Hari Murti +5 位作者 Misnawi Eka Tarwaca Susila Putra Bayu Setyawan Miftahur Rizqi Akbar Tri Rini Nuringtyas Agung Wahyu Susilo 《Beverage Plant Research》 CAS 2025年第1期308-316,共9页
Flavor is the main parameter of cocoa bean quality.The characteristic of flavor is influenced by the composition and intensity of metabolites both volatile and non volatile.It was hypothesized that non-volatile compou... Flavor is the main parameter of cocoa bean quality.The characteristic of flavor is influenced by the composition and intensity of metabolites both volatile and non volatile.It was hypothesized that non-volatile compounds impacted the flavor preference of whole cocoa beans.This study aimed to identify the differences in the non-volatile compounds between aromatic and non-aromatic genotypes.The study used the genetic materials that have been classified as aromatic and non-aromatic genotypes based on sensory characteristics.The six aromatic cocoa genotypes,including DR 2,ICCRI 03,ICCRI 07,ICCRI 09,TSH 858,and MCC 02,and four non-aromatic cocoa genotypes consisting of Sulawesi 1,Sulawesi 2,KW 516,and KEE 02 were used.The results showed that the non-volatile compounds influenced the cocoa flavor both taste and aroma.There were differences in the content of non-volatile compounds in aromatic and non-aromatic genotypes.The fermentation index,carbohydrate,and polyphenol content of the aromatic genotypes were higher than the non-aromatic group.The fermentation index showed an important indicator of the flavor precursor development of cocoa beans.Polyphenol compounds have a contribution to bitter and astringent taste.The carbohydrate and theobromine content influenced the strength of the fine aroma,such as fresh fruit,browned fruit,woody,and spicy. 展开更多
关键词 sensory cha non volatile compounds genetic materials cocoa bean polyphenols genotypes fermentation flavor
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Higher salt concentration severely affects seed germination of ten Indian genotypes of lucerne(Medicago sativa L.) 认领 引用
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作者 Sagar S.Jade Prashant A.Khedkar +2 位作者 Santosh Y.Shitole Pramodkumar S.Takawale Rahul A.Bahulikar 《Grass Research》 2025年第1期139-148,共10页
Lucerne(Medicago sativa L.)is the world's most important and ancient legume forage crop.It is moderately tolerant to salinity,but crop productivity is hampered.Salinity affects plant development,starting from seed... Lucerne(Medicago sativa L.)is the world's most important and ancient legume forage crop.It is moderately tolerant to salinity,but crop productivity is hampered.Salinity affects plant development,starting from seed germination to the reproductive stage.ISSR markers were employed to assess the genetic diversity among India's ten most common lucerne genotypes;the nine ISSR primers showed 83.50%polymorphism.The dendrogram revealed lower bootstrap support for group I;genotypes from group II were genetically distinct.Seed germination performance among the genotypes was studied at 0(control),85.55,171,256,and 342 mM NaCl concentrations.Higher germination was observed under control conditions.At a salt concentration of 85.55 mM NaCl,the genotypes under study showed less variation for germination and seedling characters.All germination indices and seedling parameters decrease with an increase in salt concentrations.Among the ten genotypes,Alamdar-51 showed high germination indices and seedling parameters under a high salinity level of 256 mM NaCl,indicating its ability for germination and initial growth under high saline conditions.At salinity concentrations of 342 mM NaCl,seed germination of all genotypes was severely affected.RL-88 and Kutchi selection showed the highest recovery of seed germination when seeds were transferred from a saline concentration to distilled water.These two genotypes demonstrated resistance to prolonged exposure to unfavorable salinized environments.This evaluation approach was found to be effective in identifying genotypes based on seed germination and early seedling growth stage under salinity stress. 展开更多
关键词 lower bootstrap support genetic diversity salinity tolerance seed germination ISSR markers issr primers genotypes lucerne
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How to select favorable and promising alleles and genotypes for breeding:A case study of rice blast resistance genes in Southern and Northeastern China 认领 引用 被引量：1
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作者 Xing Wang Jinyan Wang +8 位作者 Yaling Zhang Yongxiang Yao Ying Sun Liping Lin Xuemei Ye Rongbai Li Zhibin Liang Yizhen Deng Qinghua Pan 《The Crop Journal》 SCIE CAS CSCD 2025年第1期104-114,共11页
Asian rice comprises two major subspecies:Xian(X)and Geng(G),and the diverged resistance genes(R)have provided a foundation for breeding improved cultivars to control rice blast disease.After conducting two-phase alle... Asian rice comprises two major subspecies:Xian(X)and Geng(G),and the diverged resistance genes(R)have provided a foundation for breeding improved cultivars to control rice blast disease.After conducting two-phase allele mining using six updated FNP marker systems,the functional haplotypes at Pit,Pib,and Pi63 strictly diverged into the X-populations and were defined as X-R loci,while those at Pi54,Pi37,and Pi36 into the G-populations as G-R loci.The genic diversity at the three X-R loci(16 alleles)was twofold higher than that at the three G-R loci(8 alleles),and the allelic diversity in the Southern region(21 alleles)was nearly double that in the Northeastern region(11 alleles).Both observations reflect a significant difference in genetic diversity between X-and G-populations,and indicate that the effective R-genes mainly originated from X-subspecies.Based on the allelic structures characterized by a set of 10 parameters,8 and 16 alleles were respectively recognized as favorable and promising ones for the regional breeding programs.The genotypic structures of the two regional populations were almost different,indicating that the diverged alleles have been further assembled into two series of regional genotypes through long-term breeding programs,despite the presence of one-third of region-common alleles.The genotypic diversity in the Southern region(55 genotypes)was nearly twice as high as that in the Northeastern region(28),which perfectly reflects the aforementioned differences in both genic and allelic diversities.After analyzing the genotypic structures using a set of 13 parameters,4 and 23 genotypes,respectively,can be recommended as the favorable and promising ones for the regional breeding programs.The case study serves as a concrete sample of how to identify the favorable and promising alleles and genotypes,and beneficial parents based their comprehensive population structures for gene-designed breeding. 展开更多
关键词 Rice blast Xian and Geng type resistance genes Allelic and genotypic population structures Favorable allele and genotype Promising allele and genotype
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Actionable Genotypes and Their Association with Life Span in Iceland 认领 引用
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作者 Brynjar O Jensson 《四川生理科学杂志》 2025年第11期2486-2486,共1页
Background:In 2021,the American College of Medical Genetics and Genomics(ACMG)recommended reporting actionable genotypes in 73 genes associated with diseases for which preventive or therapeutic measures are available.... Background:In 2021,the American College of Medical Genetics and Genomics(ACMG)recommended reporting actionable genotypes in 73 genes associated with diseases for which preventive or therapeutic measures are available.Evaluations of the association of actionable genotypes in these genes with life span are currently lacking.Methods:We assessed the prevalence of coding and splice variants in genes on the ACMG Secondary Findings,version 3.0(ACMG SF v3.0),list in the genomes of 57,933 Icelanders.We assigned pathogenicity to all reviewed variants using reported evidence in the ClinVar database,the frequency of variants,and their associations with disease to create a manually curated set of actionable genotypes(variants).We assessed the relationship between these genotypes and life span and further examined the specific causes of death among carriers. 展开更多
关键词 Icelandic population genetic variants pathogenicity life span coding splice variants actionable genotypes preventive therapeutic measures ACMG secondary findings
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Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability 认领 引用 被引量：21
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作者 Zhang-yue SONG Jing-luan TIAN +6 位作者 Wei-zhe FU Lin LI Ling-hong LU Lian ZHOU Zhi-hui SHAN Gui-xiang TANG Hui-xia SHOU 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2013年第4期289-298,共10页
The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation.Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most impo... The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation.Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system.In this study,twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection,regeneration capacity,and stable transgenic efficiency.Three genotypes,Yuechun 04-5,Yuechun 03-3,and Tianlong 1,showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system.For the Tianlong 1,the average stable transformation efficiency is 4.59%,higher than that of control genotypes(Jack and Williams 82),which is enough for further genomic research and genetic engineering.While polymerase chain reaction(PCR),LibertyLink strips,and β-glucuronidase(GUS) staining assays were used to detect the insertion and expression of the transgene,leaves painted with 135 mg/L Basta could efficiently identify the transformants. 展开更多
关键词 Soybean Agrobacterium-mediated transformation Genotypes Stable transgenic efficiency
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Prevalence and Genotypes of Rotavirus A and Human Adenovirus among Hospitalized Children with Acute Gastroenteritis in Fujian, China, 2009-2017 认领 引用 被引量：12
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作者 WU Bing Shan HUANG Zhi Miao +4 位作者 WENG Yu Wei CHEN Feng Qin ZHANG Yun Lin LIN Wei Dong YU Ting Ting 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2019年第3期210-214,共5页
Gastroenteritis is an infectious diarrhea that has been considered as an important cause of hospitalizations and death in children aged < 5 years, particularly in developing countries. Unsanitary water, contaminate... Gastroenteritis is an infectious diarrhea that has been considered as an important cause of hospitalizations and death in children aged < 5 years, particularly in developing countries. Unsanitary water, contaminated food, poor hygiene, and inadequate disposal of waste and feces are all risk factors for gastroenteritis, resulting in the higher incidence in developing countries. Gastroenteritis is generally caused by viral infections, among which rotavirus (RV) infections have been reported to be the most common, especially among young children aged < 5 years with acute gastroenteritis in Asia and Africa[1]. Other viruses associated with acute gastroenteritis include human Adenovirus (HAdV), Norovirus, Sapovirus (SaV), human Astrovirus (HAstV), and Aichi virus. Recent research has reported that adenovirus types 40 and 41, belonging to species F, cause gastroenteritis and were therefore termed as enteric adenoviruses. In addition, non-enteric HAdV species such as A, B, C, and D have been associated with diarrheal. 展开更多
关键词 Prevalence and Genotypes Rotavirus A Acute Gastroenteritis
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Hepatitis C virus infections and genotypes in China 认领 引用 被引量：32
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作者 Yuan-Ding Chen Ming-Ying Liu +4 位作者 Wen-Lin Yu Jia-Qi Li Mei Peng Qing Dai Xiao Liu Zhen-Quan Zhou From the Central Laboratory, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China Kunming Hospital for Infectious Diseases, Kunming 650041, China 《Hepatobiliary & Pancreatic Diseases International》 CAS 2002年第2期194-201,共8页
Objective: To define the conditions of hepatitis C vi- rus (HCV) infections, and geographic and demo- graphic distributions of genotypes in China. Methods: HCV infected patients were selected from individuals with dif... Objective: To define the conditions of hepatitis C vi- rus (HCV) infections, and geographic and demo- graphic distributions of genotypes in China. Methods: HCV infected patients were selected from individuals with different patterns of liver diseases and high risk populations in different parts of China. Genotypes of HCV in some isolates were further ana- lyzed, based on the data from our laboratory studies and some carefully selected published literatures. Results: The anti-HCV positive rates were 9.7% in patients with acute hepatitis, 13.3% in patients with chronic hepatitis, 18.3% in patients with hepatocel- lular carcinoma, 33.0% in patients with liver cirrho- sis, and 43.2% in patients with posttransfusional hepatitis (average, 16.2% in patients with liver di- seases). The anti-HCV positive rates in the high risk populations were 36.4% in leukemic patients, 43.0% in hemodialysis patients, 12.7% in blood do- nors, 64.1% in drug abusers, 13.1% in prostitutes, and 2.57% in naturally healthy people. At least 4 clades (clades 1, 2, 3 and 6) of HCV were found in China with different geographic and demographic distributions. Genotype 1b was the most widely dis- tributed genotype, and genotype 3 was mainly found in Yunnan Province, Southwest China. Conclusion: China has a high incidence of HCV in- fection. Our results will provide a strategic basis for diagnosis, treatment and possibly prophylaxis of he- patitis C virus diseases. 展开更多
关键词 HCV infections genotypes China
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Hepatitis C virus in Pakistan:A systematic review of prevalence,genotypes and risk factors 认领 引用 被引量：17
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作者 Yasir Waheed Talha Shafi +1 位作者 Sher Zaman Safi Ishtiaq Qadri 《World Journal of Gastroenterology》 SCIE CAS 2009年第45期5647-5653,共7页
In Pakistan more than 10 million people are living with Hepatitis C virus (HCV), with high morbidity and mortality. This article reviews the prevalence, genotypes and factors associated with HCV infection in the Pakis... In Pakistan more than 10 million people are living with Hepatitis C virus (HCV), with high morbidity and mortality. This article reviews the prevalence, genotypes and factors associated with HCV infection in the Pakistani population. A literature search was performed by using the keywords; HCV prevalence, genotypes and risk factors in a Pakistani population, in Pubmed, PakMediNet and Google scholar. Ninetyone different studies dating from 1994 to May 2009 were included in this study, and weighted mean and standard error of each population group was calculated. Percentage prevalence of HCV was 4.95% ± 0.53% in the general adult population, 1.72% ± 0.24% in the pediatric population and 3.64% ± 0.31% in a young population applying for recruitment, whereas a very high 57% ± 17.7% prevalence was observed in injecting drug users and 48.67% ± 1.75% in a multi-transfused population. Most prevalent genotype of HCV was 3a. HCV prevalence was moderate in the general population but very high in injecting drug users and multi-transfused populations. This data suggests that the major contributing factors towards increased HCV prevalence include unchecked blood transfusions and reuse of injection syringes. Awareness programs are required to decrease the future burden of HCV in the Pakistani population. 展开更多
关键词 Hepatitis C virus Prevalence Genotypes Blood transfusions Injections
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Hepatitis C virus genotypes in Serbia and Montenegro: The prevalence and clinical signifi cance 认领 引用 被引量：7
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作者 Neda Svirtlih Dragan Delic +9 位作者 Jasmina Simonovic Djordje Jevtovic Ljubisa Dokic Eleonora Gvozdenovic Ivan Boricic Dragica Terzic Sladjana Pavic Gorana Neskovic Sonja Zerjav Vladimir Urban 《World Journal of Gastroenterology》 SCIE CAS 2007年第3期355-360,共6页
AIM： To investigate the prevalence of hepatitis C virus （HCV） genotypes in Serbia and Montenegro and their influence on some clinical characteristics in patients with chronic HCV infection. METHODS： A total of 164... AIM： To investigate the prevalence of hepatitis C virus （HCV） genotypes in Serbia and Montenegro and their influence on some clinical characteristics in patients with chronic HCV infection. METHODS： A total of 164 patients was investigated. Complete history, route of infection, assessment of alcohol consumption, an abdominal ultrasound, standard biochemical tests and liver biopsy were done. Gene sequencing of 5＇ NTR type-specific PCR or commercial kits was performed for HCV genotyping and subtyping. The SPSS for Windows （version 10.0） was used for univariate regression analysis with further multivariate analysis. RESULTS： The genotypes 1, 2, 3, 4, 1b3a and 1b4 were present in 57.9%, 3.7%, 23.2%, 6.7%, 6.7% and 1.8% of the patients, respectively. The genotype 1 （mainly the subtype 1b） was found to be independent of age in subjects older than 40 years, high viral load, more severe necro-inflammatory activity, advanced stage of fibrosis, and absence of intravenous drug abuse. The genotype 3a was associated with intravenous drug abuse and the age below 40. Multivariate analysis demonstrated age over 40 and intravenous drug abuse as the positive predictive factors for the genotypes lb and 3a, respectively.CONCLUSION： In Serbia and Montenegro, the genotypes 1b and 3a predominate in patients with chronic HCV infection. The subtype lb is characteristic of older patients, while the genotype 3a is common in drug abusers. Association of the subtype lb with advanced liver disease, higher viral load and histological activity suggests earlier infection with this genotype and eventually its increased pathogenicity. 展开更多
关键词 Hepatitis C virus Genotypes Prevalence Serbia Montenegro
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Changes in Phosphorus Fractions, pH, and Phosphatase Activity in Rhizosphere of Two Rice Genotypes 认领 引用 被引量：18
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作者 LI Yong-Fu LUO An-Cheng +1 位作者 WEI Xing-Hua YAO Xu-Guo 《Pedosphere》 SCIE CAS 2008年第6期785-794,共10页
A rhizobox experiment with two phosphorus (P) treatments, zero-P (0 mg P kg-1) and plus-P (100 mg P kg-1) as Ca(H2PO4)2·H2O, was conducted to study the chemical and biochemical properties in the rhizosphere of tw... A rhizobox experiment with two phosphorus (P) treatments, zero-P (0 mg P kg-1) and plus-P (100 mg P kg-1) as Ca(H2PO4)2·H2O, was conducted to study the chemical and biochemical properties in the rhizosphere of two rice genotypes (cv. Zhongbu 51 and Pembe) different in P uptake ability and their relationship with the depletion of soil P fractions. Plant P uptake, pH, phosphatase activity, and soil P fractions in the rhizosphere were measured. Both total dry weight and total P uptake of Pembe were significantly (P < 0.05) higher than those of Zhongbu 51 in the zero-P and plus-P treatments. Significant depletions of resin-Pi, NaHCO3-Pi, NaHCO3-Po, and NaOH-Pi, where Pi stands for inorganic P and Po for organic P, were observed in the rhizosphere of both Zhongbu 51 and Pembe under both P treatments. Pembe showed a greater ability than Zhongbu 51 in depleting resin-Pi, NaHCO3-Pi, NaHCO3-Po, NaOH-Pi, and NaOH- Po in the rhizosphere. HCl-Pi and residual-P were not depleted in the rhizosphere of both genotypes, regardless of P treatments despite significant acidification in the rhizosphere of Pembe under zero-P treatment. Higher acid phosphatase (AcPME) activity and alkaline phosphatase (AlPME) activity were observed in the rhizosphere of both Zhongbu 51 and Pembe compared to the corresponding controls without plant. AcPME activity was negatively (P < 0.01) correlated to NaHCO3-Po concentration in the rhizosphere of both Zhongbu 51 and Pembe, suggesting that AcPME was associated with the mineralization of soil organic P. 展开更多
关键词 phosphatase phosphorus fractions rhizosphere rice genotypes soil pH
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Screening and evaluation of reliable traits of upland cotton(Gossypium hirsutum L.)genotypes for salt tolerance at the seedling growth stage 认领 引用 被引量：10
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作者 SIKDER Ripon Kumar WANG Xiangru +6 位作者 JIN Dingsha ZHANG Hengheng GUI Huiping DONG Qiang PANG Nianchang ZHANG Xiling SONG Meizhen 《Journal of Cotton Research》 CAS 2020年第2期90-102,共13页
Background:Salt stress significantly inhibits the growth,development,and productivity of cotton because of osmotic,ionic,and oxidative stresses.Therefore,the screening and development of salt tolerant cotton cultivars... Background:Salt stress significantly inhibits the growth,development,and productivity of cotton because of osmotic,ionic,and oxidative stresses.Therefore,the screening and development of salt tolerant cotton cultivars is a key issue towards sustainable agriculture.This study subjected 11 upland cotton genotypes at the seedling growth stage to five different salt concentrations and evaluated their salt tolerance and reliable traits.Results:Several morpho-physiological traits were measured after 10 days of salinity treatment and the salt tolerance performance varied significantly among the tested cotton genotypes.The optimal Na Cl concentration for the evaluation of salt tolerance was 200 mmol·L-1.Membership function value and salt tolerance index were used to identify the most consistent salt tolerance traits.Leaf relative water content and photosynthesis were identified as reliable indicators for salt tolerance at the seedling stage.All considered traits related to salt tolerance indices were significantly and positively correlated with each other except for malondialdehyde.Cluster heat map analysis based on the morpho-physiological salt tolerance-indices clearly discriminated the 11 cotton genotypes into three different salt tolerance clusters.Cluster I represented the salt-tolerant genotypes(Z9807,Z0228,and Z7526)whereas clusters II(Z0710,Z7514,Z1910,and Z7516)and III(Z0102,Z7780,Z9648,and Z9612)represented moderately salttolerant and salt-sensitive genotypes,respectively.Conclusions:A hydroponic screening system was established.Leaf relative water content and photosynthesis were identified as two reliable traits that adequately represented the salt tolerance of cotton genotypes at the seedling growth stage.Furthermore,three salt-tolerant genotypes were identified,which might be used as genetic resources for the salt-tolerance breeding of cotton. 展开更多
关键词 Cotton genotypes Salt stress Screening Membership function value Cluster analysis
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Does copper reduce cadmium uptake by different rice genotypes? 认领 引用 被引量：9
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作者 CUI Yujing ZHANG Xuhong ZHU Yongguan 《Journal of Environmental Sciences》 SCIE EI CAS 2008年第3期332-338,共7页
A hydroponics experiment was conducted to investigate the effect of copper （Cu） on cadmium （Cd）, calcium （Ca）, iron （Fe）, and zinc （Zn） uptake by several rice genotypes. The experiment was carried out as a 2... A hydroponics experiment was conducted to investigate the effect of copper （Cu） on cadmium （Cd）, calcium （Ca）, iron （Fe）, and zinc （Zn） uptake by several rice genotypes. The experiment was carried out as a 2×2×4 factorial with four rice genotypes and two levels of Cu and Cd in nutrient solution. Plants were grown in a growth chamber with controlled environment. The results showed a significant difference between the biomass of different rice genotypes （P 〈 0.001）. The Cd and Cu concentration in the solution had no significant effect on the biomass. The addition of Cu significantly decreased Cd uptake by shoots and roots of rice （P 〈 0.001）. The Cd concentration did not significantly influence Ca uptake by plants, whereas the Cu concentration did （P = 0.034）. There was a significant influence of Cd on Fe uptake by shoots and roots （P 〈 0.001, P = 0.003, respectively）. Zn uptake decreased significantly as the addition of Cd and Cu increased in shoots. We concluded that Cu had significant influence on Cd uptake. The possible mechanisms were discussed. 展开更多
关键词 cadmium calcium copper food chain genotypes zinc
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Evaluation of common bean(Phaseolus vulgaris L.)genotypes for drought stress adaptation in Ethiopia 认领 引用 被引量：4
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作者 Kwabena Darkwa Daniel Ambachew +2 位作者 Hussein Mohammed Asrat Asfaw Matthew W.Blair 《The Crop Journal》 SCIE CSCD 2016年第5期367-376,共10页
Drought stress linked with climate change is one of the major constraints faced by common bean farmers in Africa and elsewhere. Mitigating this constraint requires the selection of resilient varieties that withstand d... Drought stress linked with climate change is one of the major constraints faced by common bean farmers in Africa and elsewhere. Mitigating this constraint requires the selection of resilient varieties that withstand drought threats to common bean production.This study assessed the drought response of 64 small red-seeded genotypes of common bean grown in a lattice design replicated twice under contrasting moisture regimes,terminal drought stress and non-stress, in Ethiopia during the dry season from November2014 to March 2015. Multiple plant traits associated with drought were assessed for their contribution to drought adaptation of the genotypes. Drought stress determined by a drought intensity index was moderate(0.3). All the assessed traits showed significantly different genotypic responses under drought stress and non-stress conditions. Eleven genotypes significantly(P ≤ 0.05) outperformed the drought check cultivar under both drought stress and non-stress conditions in seed yielding potential. Seed yield showed positive and significant correlations with chlorophyll meter reading, vertical root pulling resistance force, number of pods per plant, and seeds per pod under both soil moisture regimes, indicating their potential use in selection of genotypes yielding well under drought stress and non-stress conditions. Clustering analysis using Mahalanobis distance grouped the genotypes into four groups showing high and significant inter-cluster distance, suggesting that hybridization between drought-adapted parents from the groups will provide the maximum genetic recombination for drought tolerance in subsequent generations. 展开更多
关键词 Climate resilient varieties Correlation analysis Drought-adapted common bean genotypes Multiple adaptive traits
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Sodium and Potassium Absorption and Distribution in Relation to Growth and Internal Potassium Use Efficiency of K-efficient and -inefficient rice genotypes 认领 引用 被引量：5
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作者 LIU JIANXIANG YANG XIAOE +1 位作者 NI WUZHONG and YANG YUAI College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310029 《Pedosphere》 SCIE CAS 2001年第3期235-242,共8页
A pot experiment with two rice (Oriza sativa L.) genotypes differing in internal potassium use efficiency (IKUE) was conducted under different sodium (Na) and potassium (K) levels. Adding NaCl at a proper level enhanc... A pot experiment with two rice (Oriza sativa L.) genotypes differing in internal potassium use efficiency (IKUE) was conducted under different sodium (Na) and potassium (K) levels. Adding NaCl at a proper level enhanced rice vegetative growth and increased grain yield and IKUE under low potassium. Addition of higher rate of NaCl had a negative effect on the growth of the K-efficient rice genotype, but did not for the K-inefficient genotype. Under low-K stress, higher NaCl decreased IKUE of the K-efficient rice genotype but increased IKUE for the K-inefficient genotype. At tillering stage and under low-K stress, adding NaCl increased K and Na contents and decreased the ratio of K/Na for both genotypes. At harvesting stage under low-K stress, adding NaCl increased K and Na contents and K/Na ratio for the K-efficient genotype but decreased the K/Na ratio for the K-inefficient genotype. The accumulated Na was mostly deposited in the roots and sheaths. At tillering stage, the K and Na contents and the K/Na ratios in different parts for both genotypes decreased in the following sequence: K+ in sheaths > K+ in blades > K+ in roots; Na+ in roots > Na+ in sheaths > Na+ in blades; and K/Na in sheaths 》 K/Na in roots. The K-efficient genotype had a lower K/Na ratio in roots and sheaths than the K-inefficient genotype under low-K stress. At harvesting stage, K and Na contents in grains were not affected, whereas K/Na ratio in the rice straws was increased for the K-efficient genotype but decreased for the K-inefficient genotype by Na addition. However, this was not the case under K sufficient condition. 展开更多
关键词 distribution internal K use efficiency (IKUE) K Na rice genotypes
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