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The correlation between characteristics and pharmacological effects of monoterpene glycosides and tannins in Radix Paeoniae Alba 认领 引用
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作者 Qitong Zheng Mengyao Chen +4 位作者 Jialiang Ying Zhichao Wang Qiyuan Shan Xia-Nan Sang Gang Cao 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2026年第3期668-691,共24页
The pharmacodynamic material basis constitutes the central element of traditional Chinese medicine(TCM)in disease treatment.By summarizing the active compounds'characteristics,bioavailability,pharmacological effec... The pharmacodynamic material basis constitutes the central element of traditional Chinese medicine(TCM)in disease treatment.By summarizing the active compounds'characteristics,bioavailability,pharmacological effects,and molecular mechanisms,we can explain the complex interactions between TCMs and diseases.Previous studies have demonstrated that monoterpene glycosides and tannins are related to the pharmacological activity of Radix Paeoniae Alba(RPA).However,research on RPA has primarily focused on monoterpene glycosides,and the functional role of tannins in RPA has received little attention.Observations from animal studies indicate that monoterpene glycosides and tannins exhibit poor bioavailability.Carboxylesterase,produced by gut microbiota,is crucial for metabolizing these compounds in the intestine.Monoterpene glycosides and their gut metabolites can be absorbed into the bloodstream,exerting various pharmacological effects,including anti-inflammatory,immunomodulatory,and neuromodulator activities.In contrast,tannins consist of highly hydrophobic polyphenols that form insoluble protein-tannin complexes.Due to their inability to cross the intestinal barrier,tannins primarily exert localized pharmacological effects within the digestive system.This study systematically reviews the pharmacological activities and mechanisms of monoterpene glycosides and tannins in RPA,while establishing their therapeutic contributions to the herb's pharmacological effects. 展开更多
关键词 Material basis Radix Paeoniae Alba Monoterpene glycosides Tannins Pharmacological action
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Influence of steviol glycosides on the stability of anthocyanins during storage of blueberry juice 认领 引用
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作者 Chunxue Zhang Lin Zhang +4 位作者 Xinmei Yun Ahmed Adel Ashour Hui Tan Yuehua Wang Xinyao Jiao 《Food Innovation and Advances》 2026年第2期219-229,共11页
Blueberry juice maintains the nutritional value of fresh berries while providing their complex flavor profile.Anthocyanins contribute to the vibrant color of blueberry juice and exhibit physiological functions.Steviol... Blueberry juice maintains the nutritional value of fresh berries while providing their complex flavor profile.Anthocyanins contribute to the vibrant color of blueberry juice and exhibit physiological functions.Steviol glycosides are widely utilized as a natural sweetener in fruit beverages,and investigating their regulatory role in the degradation of anthocyanins within juice systems is of considerable importance.This study aimed to examine the effects of steviol glycosides on the color,anthocyanin content,antioxidant capacity,and in vitro simulated digestion stability of anthocyanins in blueberry juice during storage.The juice was stored for 90 d at 4 and 25℃in dark conditions.Throughout storage,the juice exhibited color fading,accompanied by a loss of its original color intensity.Notably,the sample supplemented with 20 mg/100 mL steviol glycosides showed the least color change when stored at 4℃.The addition of steviol glycosides resulted in a reduced degradation rate constant(k)and an extended half-life(t1/2)of anthocyanins.Thermodynamic analysis indicated that steviol glycosides enhanced the thermal stability of anthocyanins in blueberry juice.Furthermore,blueberry juice containing 12 and 16 mg/100 mL steviol glycosides demonstrated the highest hydroxyl radical scavenging capacity at 4℃,and the strongest ABTS radical scavenging activity at 25℃,respectively.During in vitro simulated digestion,the sample with 4 mg/100 mL steviol glycosides exhibited the highest retention rate of total anthocyanins.Molecular docking analysis revealed the formation of hydrophobic interactions and hydrogen bonds between stevioside and cyanidin-3-O-glucoside,as well as malvidin-3-O-galactoside.The findings of this study provide a solid foundation for advancing fruit juice processing technologies. 展开更多
关键词 antioxidant capacity vitro digestion anthocyanins color stability juice systems steviol glycosides blueberry juice
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Total glycosides of Cistanche deserticola enhance the inhibitory effect of Sorafenib on mice with hepatocellular carcinoma 认领 引用
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作者 Duo Feng Jing Wang +6 位作者 Shaoshi Zhang Shiqi Zhou Mengjie Li Xianchun Wang Yu Guo Jian Zhao Wenjie Yan 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第7期3168-3180,共13页
Long-term use of Sorafenib(SOR)in patients with hepatocellular carcinoma will lead to drug resistance,leading to diarrhea,weight loss and other adverse reactions.Cistanche deserticola,as a homologous substance of medi... Long-term use of Sorafenib(SOR)in patients with hepatocellular carcinoma will lead to drug resistance,leading to diarrhea,weight loss and other adverse reactions.Cistanche deserticola,as a homologous substance of medicine and food,its total glycosides(TG)have anti-hepatocellular carcinoma activity.This paper aimed to explore the anti-hepatocellular carcinoma effect of TG combined with SOR on mice and its mechanism.In this study,according to the previous research of experts,the dosage of 30 mg/kg SOR was selected,aiming at giving consideration to the anti-tumor effect and reducing toxicity.Specifically,HepG2 cells were used to establish a subcutaneous tumorigenic model.Histological changes,oxidative stress,immune regulation and intestinal metabolism and microbial composition of mice were detected.It was found TG did not affect the normal growth of mice without any toxic side effects and could synergize with SOR to inhibit tumor growth.Hematoxylin-eosin results found mice in TG group had more complete hepatocyte structure and less pathological changes in liver.The tumor tissue cells became rare and necrotic.TUNEL staining results revealed that TG could significantly promote apoptosis of HepG2 cells,but at a lower level than SOR.In addition,the content of catalase(CAT),superoxide dismutase(SOD)and glutamic-pyruvic transaminase(ALT)increased,aspartate aminotransferase(AST)and alkaline phosphatase(ALP)decreased,indicating TG could effectively reduce liver lesions.TG could also decrease the content of interleukin-2(IL-2),interleukin-17(IL-17),tumor necrosis factor-α(TNF-α),etc.,and increase interferon-γ(IFN-γ),granulocyte-macrophage colony stimulating factor(GM-CSF).Meanwhile,immunohistochemistry was used to observe the decreased expression of Ki67,β-catenin and dishevelled(Dsh)proteins and the increased GSK-3βin the tumor tissues,it was presumed that TG exerted its anti-hepatocellular carcinoma activity through the Wnt/β-catenin signaling pathway.Most importantly,TG could synergize with SOR to regulate metabolic levels in the gut,balance intestinal microbial composition,and increase the abundance of beneficial intestinal bacteria.In conclusion,the results showed that TG(64 mg/kg)could assist SOR(30 mg/kg)to inhibit the development of hepatocellular carcinoma,provide some theoretical basis and technical support.C.deserticola,as a homologous substance of medicine and food,its combination with SOR can provide a new idea for the treatment of hepatocellular carcinoma and its future application potential is worth further exploration and development. 展开更多
关键词 Cistanche deserticola Anti-hepatocellular carcinoma Total glycosides Sorafenib Wnt/β-catenin signaling pathway
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CsUGT89A2 enhances tea plant resistance to Toxoptera aurantia by mediating flavonoid glycosides biosynthesis 认领 引用 被引量：1
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作者 Xingrong Zhou Dingli Chen +11 位作者 Fengyun Tian Jie Ma Mei Chen Youshudi Xie Changli Yang Yanglin Liang Houhong Xiao Xue Dong Di Yang Yingqin He Xinlong Dai Yan Li 《Horticulture Research》 SCIE CSCD 2025年第11期147-160,共14页
Tea plant[Camellia sinensis(L.)O.Kuntze]is a globally important crop but is severely threatened by Toxoptera aurantia infestations,which impact yield and safety.However,the response of tea plants to aphid feeding rema... Tea plant[Camellia sinensis(L.)O.Kuntze]is a globally important crop but is severely threatened by Toxoptera aurantia infestations,which impact yield and safety.However,the response of tea plants to aphid feeding remains largely unexplored.This study investigates the feeding behavior of T.aurantia on different cultivars and identifies‘Huangjinya’and‘Qiancha 1’as susceptible and resistant cultivars,respectively.Transcriptome analysis revealed that CsUGT89A2 was significantly upregulated in response to T.aurantia infestation.In vitro biochemical assays demonstrated that CsUGT89A2 encodes a f lavonoid 7-glycosyltransferase that catalyzes the conversion of f lavonoids and UDP-glucose into f lavonoid 7-O-glucosides.In vivo,silencing CsUGT89A2 significantly reduced f lavonoid glycoside accumulation.To further clarify the role of CsUGT89A2 in tea plant resistance to T.aurantia,we used tobacco and tea f lowers to evaluate aphid feeding and reproduction under chemical treatment,gene silencing,and gene overexpression conditions.Statistical analysis showed that,compared with f lavonoids,the application of f lavonoid 7-O-glycosides significantly reduced T.aurantia reproductive capacity.Furthermore,compared with the control,overexpression of CsUGT89A2 significantly reduced the reproductive ability of aphids,while its silencing increased reproductive rates.Overall,our findings demonstrate that CsUGT89A2 mediates f lavonoid glycosylation and enhances insect resistance in tea plants by increasing f lavonoid glycoside levels,offering new insights into the role of f lavonoid glycosides in the insect resistance of C.sinensis. 展开更多
关键词 aphid feeding csugt insect resistance tea plants feeding behavior flavonoid glycosides toxoptera aurantia
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Effects of Qingyangshen glycosides on neuroplasticity in a mouse model of social defeat 认领 引用 被引量：1
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作者 Jingru Wang Weishi Chen +4 位作者 Qiang Zhu Yao Liu Zheng Kang Dingding Liu Guirong Zeng 《Animal Models and Experimental Medicine》 CAS CSCD 2025年第4期581-594,共14页
Background:Qingyangshen(Cynanchum otophyllum C.K.Schneid)is a folk drug for treating depression and other mental disorders induced by social defeat stress.Neuroplasticity in the hippocampus is essential for the modula... Background:Qingyangshen(Cynanchum otophyllum C.K.Schneid)is a folk drug for treating depression and other mental disorders induced by social defeat stress.Neuroplasticity in the hippocampus is essential for the modulation of cognition and emotion,and its impairment may contribute to the development and progression of depression.Our previous studies have found that Qingyangshen glycosides(QYS)can improve depression-like behavior in social failure mouse models,mainly through PGC-1α/FNDC5/BDNF signaling pathways activation,but its effects and mechanisms on hippocampal neuroplasticity remain unknown.Methods:Chronic social defeat stress(CSDS)was used to induce social defeat in mice.Morphological changes in the hippocampus were observed by H&E staining and Golgi staining.Immunofluorescence double staining was used to detect the expression of synaptophysin(SYN)and postsynaptic density protein-95(PSD-95),while western blot was employed to evaluate PSD-95,SYN,and doublecortin(DCX)proteins.The pathological processing of social defeat and the therapeutic effects of QYS on it was confirmed through behavioral assessment associated with morpho-logic observation.Results:During the whole study,the sucrose preference indices and OFT activity time of CSDS mice were significantly decreased(p≤0.05),and the tail suspension immobil-ity time was significantly increased(p≤0.05),suggesting that the mice had significant depressive symptoms.Treatment with QYS(25,50,and 100 mg/kg)significantly al-leviated depressive symptoms in CSDS mice,which was demonstrated by significantly(p≤0.05 or p≤0.01)reducing the duration of tail-hanging immobility and increasing the tendency of sucrose preference indices and OFT activity time.QYS treatment also significantly increased the expression of DCX,PSD-95,and SYN proteins,which play a crucial role in depression.Conclusions:QYS alleviated these symptoms by enhancing hippocampal neuroplasti-city through upregulating the expression of synapse-associated proteins(SAPs).The therapeutic mechanism of QYS may involve modulating the neuroplasticity of hip-pocampus neurons by altering the expression of SAPs. 展开更多
关键词 chronic social defeat DCX neuroplasticity PSD-95 QYS glycosides SYN
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Anti-cancer and anti-inflammatory effects of flavan-4-ol and flavan glycosides from the roots of Pronephrium penangianum 认领 引用
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作者 Feibing Huang Yong Yang +5 位作者 Qingling Xie Hanwen Yuan Muhammad Aamer Yuqing Jian Ye Zhang Wei Wang 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2025年第5期593-603,共11页
Five new flavan-4-ol glycosides jixueqiosides A-E(1-5)and two new flavan glycosides jixueqiosides F and G(6 and 7),along with twelve known flavan-4-ol glycosides(8-19),were isolated from the roots of Pronephrium penan... Five new flavan-4-ol glycosides jixueqiosides A-E(1-5)and two new flavan glycosides jixueqiosides F and G(6 and 7),along with twelve known flavan-4-ol glycosides(8-19),were isolated from the roots of Pronephrium penangianum.Comprehensive spectral analyses,X-ray single-crystal diffraction,and theoretical electronic circular dichroism(ECD)calculations established structures and absolute configurations.A single crystal structure of flavan-4-ol glycoside(14)was reported for the first time,while the characteristic ECD and NMR data for all isolated flavan-4-ol glycosides(1-5,8-19)were analyzed,establishing a set of empirical rules.Activity screening of these isolates showed that 8 and 9 could inhibit the proliferation of MDA-MB-231 and MCF-7 cells with IC50 values of 7.93±2.85μmol·L-1and 5.87±1.58μmol·L-1(MDA-MB-231),and 2.21±1.38μmol·L-1and 3.52±1.55μmol·L-1(MCF-7),respectively.Western blotting and flow cytometry analyses demonstrated that 8 and 9 dose-dependently induced apoptosis in MDA-MB-231 cells by up-regulating BAX,activating caspase-3 and down-regulating BCL-2.Additionally,compound 8 affected autophagy-related proteins,increasing the ratio of LC3-II/LC3-I and Beclin-1 levels to inhibit MDA-MB-231 cell proliferation.Moreover,anti-inflammatory studies indicated that 2,3,7,13,14,and 18 moderately inhibited tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and nitric oxide(NO)release. 展开更多
关键词 Pronephrium penangianum Tujia ethnomedicine Jixueqi Flavan-4-ol and Flavan glycosides Anti-cancer Anti-inflammatory
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MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava 认领 引用
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作者 Mengtao Li Xiao Zhao +9 位作者 Yajun Li Yuanchao Li Xiaoye Zhao Weitao Mai Luqman Khan Qibing Liang Qingchun Yin Wenquan Wang Jinping Liu Xin Chen 《The Crop Journal》 SCIE CAS CSCD 2025年第1期204-214,共11页
The high content of cyanogenic glycosides(CG)in cassava tubers affects food safety.CG are involved in the plant growth and development and protect cassava leaves from herbivorous predators.However,the regulatory mecha... The high content of cyanogenic glycosides(CG)in cassava tubers affects food safety.CG are involved in the plant growth and development and protect cassava leaves from herbivorous predators.However,the regulatory mechanism of CG biosynthesis remains poorly understood.Here,yeast one-hybrid assays were performed using a mixed cDNA library of cassava tubers and leaves as prey and the promoter of MeCYP79D2 as bait.MeCYP79D2,a cytochrome P450 protein,is the rate-limiting enzyme for CG synthesis in cassava.From this information,a candidate regulator of MeCYP79D2 was selected and identified as transcription factor MePHD1.2.MePHD1.2,located in the nucleus and exhibiting an inhibitory transcription activity directly bound to an AT-rich motif in the promoter of MeCYP79D2.In cassava,the transcriptional activity of MeCYP79D2 was considerably enhanced in mephd1.2 mutant lines leading to increased linamarin and lotaustralin contents.Deletion of MePHD1.2 promoted the production of CGs in cassava and decreased transcription inhibition on MeCYP79D2,exposing a novel regulatory module governing biosynthesis of CGs. 展开更多
关键词 Cyanogenic glycosides Cytochrome P450 Manihot esculenta MePHD1.2
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Flavonol glycosides in tea:the role of mitigating oxidative stress during thewithering process 认领 引用 被引量：1
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作者 M.Iqbal Prawira-Atmaja Sirima Puangpraphant 《Beverage Plant Research》 CAS 2025年第1期90-102,共13页
Withering is a crucial step in tea production that reduces the moisture content and induces biochemical changes in tea leaves.The withering process triggers oxidative stress by accumulating reactive oxygen species(ROS... Withering is a crucial step in tea production that reduces the moisture content and induces biochemical changes in tea leaves.The withering process triggers oxidative stress by accumulating reactive oxygen species(ROS)in tea leaves.The presence of flavonol glycosides is crucial for tea quality and tea's stress response to environmental changes pre-and post-harvest.However,the role of flavonol glycosides during withering remains unexplored.The degradation of flavonol glycosides during withering is mainly attributed to the enzymatic antioxidant defense system,which produces flavonol aglycones and soluble sugars that protect leaf cells from oxidative stress.This degradation contributes to a sweeter taste and enhances the taste and aroma of tea.This review emphasizes the role of the withering process and the degradation of flavonol glycosides,highlighting their role in neutralizing ROS formation as a response to oxidative stress,and discusses how changes in flavonol glycosides contribute to the taste of tea.The importance of the withering process in the degradation of flavonol glycosides is also highlighted.Further research is needed to understand the dynamic changes of flavonol glycosides during the withering process,which is essential to improving tea quality. 展开更多
关键词 reactive oxygen species ros biochemical changes flavonol glycosides tea quality oxidative stress reduces moisture content withering process
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Asprecosides A-J,ten new pentacyclic triterpenoid glycosides with cytotoxic activity from the roots of Ilex asprella 认领 引用 被引量：1
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作者 Yuwei Wu Baihui Zhang +4 位作者 Wenxian Li Lihua Peng Weilin Qiao Wei Li De-an Guo 《Natural Products and Bioprospecting》 CSCD 2025年第2期14-24,共11页
Phytochemical study of the n-BuOH extract of Ilex asprella resulted in the discovery of ten new pentacyclic triterpenoid glycosides,comprising nine ursane-type glycosides(1-9)and one oleanane-type glycoside(10),along ... Phytochemical study of the n-BuOH extract of Ilex asprella resulted in the discovery of ten new pentacyclic triterpenoid glycosides,comprising nine ursane-type glycosides(1-9)and one oleanane-type glycoside(10),along with seven known compounds(11-17).Compound 1 is the first reported 19,22-epoxy ursane triterpenoid glycoside,whereas 4 and 5 are rare examples of ursane triterpenoid glycosides containing a 28,19-lactone group.The structural characterization of these compounds was achieved using spectroscopic and chemical techniques,as well as single-crystal X-ray analysis.Compounds 7,12,15,and 17 exhibited moderate cytotoxic activities against H1975 and HCC827 cancer cells. 展开更多
关键词 Ilex asprella Pentacyclic triterpenoid glycoside Ursane Oleanane Cytotoxic activity
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Genome-wide screen and multi-omics analysis reveal OGT1 participate in the biosynthesis of safflower flavonoid glycosides 认领 引用
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作者 Bin Xian Yanxun Zhou +8 位作者 Yueying Hu Yanni Peng Xiaominting Song Ziqing Xi Yuhang Li Jie Yan Chaoxiang Ren Jin Pei Jiang Chen 《Horticulture Research》 SCIE CSCD 2024年第12期117-131,共15页
Safflower,an economic crop,is renowned for its flowers,which are widely used in medicines for treating cardiovascular and cerebrovascular diseases and in dyes for food and industry.The utility of safflower depends on ... Safflower,an economic crop,is renowned for its flowers,which are widely used in medicines for treating cardiovascular and cerebrovascular diseases and in dyes for food and industry.The utility of safflower depends on its flavonoid glycosides.Therefore,the biosynthesis of safflower flavonoid glycosides has been a focus of attention,but the present mechanisms remain poorly understood.This study aims to identify functional genes associated with flavonoid glycoside biosynthesis in safflower through a comprehensive approach that integrates whole-genome screen and multi-omics correlation studies.CYP and UGT are two crucial gene families involved in flavonoid glycoside biosynthesis.We have screened 264 CYP genes and 140 UGT genes in the genome of safflower and conducted analyses including phylogenetic relationships,conserved motifs,gene structures,cis-acting elements,and chromosome mapping,which provided extensive and comprehensive data on the CYP and UGT gene families.Integration of phenotype and metabolic data from different safflower tissues helped narrow down the screening by confirming that HSYA is synthesized only in flowers.Based on the gene expression patterns and phylogenetic analysis,CtOGT1 was ultimately identified,which could catalyze the generation of glycosides using various flavonoid substrates and exhibited strong substrate affinity.Moreover,molecular docking studies elucidated CtOGT1’s highly active intrinsic mechanism.In conclusion,this study effectively identified genes responsible for flavonoid glycoside biosynthesis in safflower through the integration of whole-genome screen and multi-omics analysis,and established a comprehensive foundation of data,methodology,and experimental evidence for further elucidating the pathways of safflower flavonoid glycoside biosynthesis. 展开更多
关键词 identify functional genes associated OGT flavonoid glycosidesthereforethe flavonoid glycosides multi omics analysis medicines treating cardiovascular cerebrovascular diseases genome wide screen safflower
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Identification and screening of cardiac glycosides in Streptocaulon griffithii using an integrated data mining strategy based on high resolution mass spectrometry 认领 引用 被引量：6
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作者 ZHU Xiao-Yu LIU Jia-Zhuo +2 位作者 DONG Zhen-Huan FENG Feng LIU Wen-Yuan 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2018年第7期546-560,共15页
The present study was designed to develop a practical strategy to tackle the problem of lacking standard compounds and limited references for identifying structure-related compounds in Streptocaulon griffithii Hook. f... The present study was designed to develop a practical strategy to tackle the problem of lacking standard compounds and limited references for identifying structure-related compounds in Streptocaulon griffithii Hook. f., especially those in trace concentrations, with a focus on antitumor activity. The cardiac glycosides(CGs)-enriched part was determined using in vitro bioactive assays in three cancer cell lines and then isolated using macroporous resins. The MS and MS/MS data were acquired using a high performance liquid chromatography coupled with hybrid quadrupole-time of flight(HPLC-Q-TOF-MS) system. To acquire data of trace compound in the extract, a multiple segment program was applied to modify the HPLC-Q-TOF-MS method. A mass defect filter(MDF) approach was employed to make a primary MS data filtration. Utilizing a MATLAB program, the redundant peaks obtained by imprecise MDF template calculated with limited references were excluded by fragment ion classification, which was based on the ion occurrence number in the MDF-filtered total ion chromatograms(TIC). Additionally, the complete cleavage pathways of CG aglycones were proposed to assist the structural identification of 29 common fragment ions(CFIs, ion occurrence number ≥ 5) and diagnostic fragment ions(DFIs, ion occurrence number < 5). As a result, 30 CGs were filtered out from the MDF results, among which 23 were identified. This newly developed strategy may provide a rapid and effective tool for identifying structure-related compounds in herbal medicines. 展开更多
关键词 Streptocaulongriffithii Cardiac glycosides Mass defect filter HPLC-Q-TOF-MS
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Pharmacokinetic and tissue distribution studies of paeoniflorin and albiflorin in rats after oral administration of total glycosides of paeony by HPLC-MS/MS 认领 引用 被引量：11
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作者 赵敏敏 高萌 +5 位作者 田玉路 杜英峰 王春英 许慧君 张兰桐 王巧 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2014年第6期403-411,共9页
A simple, sensitive and specific high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）method combined with solid phase extraction has been developed and validated for the simultaneous quantifi... A simple, sensitive and specific high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）method combined with solid phase extraction has been developed and validated for the simultaneous quantification of paeoniflorinand albiflorin in rats plasma and tissue homogenate after administration of total glycosides of paeony （TGP）. Chromatographicseparation was achieved on a C15 column （150 mmx4.6 mm, 5 pro） with mobile phase consisted of acetonitrile-0.05% formicacid aqueous （20： 80, v/v） at a flow rate of 0.5 mL/min. The analytes were detected with triple-quadrupole mass spectrometer usingturbo ion spray with negative ionization in the multiple reaction-monitoring （MRM） modes. The results of method validationconformed to the requirements for the determination of biological samples. This method was successfully applied to thepharmacokinetics and tissue distribution study of TGP in rats. The results showed that paeonifiorin and albiflorin wereabsorbed and reached peak value quickly, and had the similar pharmacokinetic characteristics. Both of them underwent a rapid andwide distribution in the tissues throughout the whole body, among which stomach was the main distribution tissue. Moreover, theyhad the ability to cross the blood-brain barrier. 展开更多
关键词 Total glycosides of paeony Solid-phase extraction Albiflorin Paeoniflorin HPLC-MS/MS
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Fingerprint analysis of Zhimu-Huangbai herb pair and simultaneous determination of its alkaloids, xanthone glycosides and steroidal saponins by HPLC-DAD-ELSD 认领 引用 被引量：8
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作者 ZHANG Feng YANG Qi +3 位作者 SUN Lian-Na GAO Shou-Hong TAO Xia CHEN Wan-Sheng 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2014年第7期525-534,共10页
AIM: To develop and validate a high performance liquid chromatography(HPLC) coupled with diode array and evaporative light scattering detectors(DAD-ELSD) method for the quantitative determination and fingerprint analy... AIM: To develop and validate a high performance liquid chromatography(HPLC) coupled with diode array and evaporative light scattering detectors(DAD-ELSD) method for the quantitative determination and fingerprint analysis of ten active constituents in three chemical classes(namely, xanthone glycosides, steroidal saponins, and alkaloids) in Zhimu-Huangbai herb pair(ZB). METHOD: Chromatographic separation was performed on a Diamonsil C18 column(4.6 mm × 250 mm, 5 μm, Dikma) by gradient elution using acetic acid in acetonitrile solution at a flow rate of 1.0 mL·min–1 at 260 nm. The drift tube temperature of ELSD was set to 60 ℃ and nebulizer gas pressure was 4.0 Bar. Method validation was performed to assure its linearity, limits of detection and quantification, precision, repeatability, stability, and accuracy. RESULTS: The HPLC-DAD-ELSD method allowed the quantification of ten compounds(phellodendrine, jatrorrhizine, palmatine, berberine, neomangiferin, mangiferin, timosaponin E-I, timosaponin B-II, timosaponin B, and timosaponin A-III), and was successfully applied to fingerprint analysis for ten batches of ZB samples. CONCLUSION: This was the first time to apply the combination of DAD and ELSD for the simultaneous determination of ten active ingredients in ZB. The results showed that the combination of quantitative analysis for marker ingredients and chemical fingerprint for the TCM herb pair provides a potentially powerful, widely introduced, and internationally accepted strategy for assessment of complex TCM formulas. 展开更多
关键词 Anemarrhena asphodeloides Phellodendron amurense Chromatographic fingerprint HPLC-DAD-ELSD Xanthone glycosides Steroidal saponins Alkaloids Simultaneous determination
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Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type Ⅱ cytoskeletal 6A in a rat model of vascular dementia 认领 引用 被引量：6
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作者 Yan-mei Zhang Wei Wu +2 位作者 Wei Ma Fang Wang Jun Yuan 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第7期1152-1158,共7页
Glycosides of Cistanche（GC）is a preparation used extensively for its neuroprotective effect against neurological diseases,but its mechanisms of action remains incompletely understood.Here,we established a bilateral ... Glycosides of Cistanche（GC）is a preparation used extensively for its neuroprotective effect against neurological diseases,but its mechanisms of action remains incompletely understood.Here,we established a bilateral common carotid artery occlusion model of vascular dementia in rats and injected the model rats with a suspension of GC（10 mg/kg/day,intraperitoneally）for 14 consecutive days.Immunohistochemistry showed that GC significantly reduced p-tau and amyloid beta（Aβ）immunoreactivity in the hippocampus of the model rats.Proteomic analysis demonstrated upregulation of mitochondrial precursor protein and downregulation of keratin type II cytoskeletal6A after GC treatment compared with model rats that had received saline.Western blot assay confirmed these findings.Our results suggest that the neuroprotective effect of GC in vascular dementia occurs via the promotion of neuronal cytoskeleton regeneration. 展开更多
关键词 nerve regeneration vascular dementia glycosides of Cistanche mitochondrial precursor protein keratin type Ⅱ cytoskeletal 6A proteomics neuroprotection neural regeneration
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Two new secoiridoid glycosides from the roots of Picrorhiza scrophulariiflora 认领 引用 被引量：10
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作者 Lian Chun Zou Tong Fei Zhu +3 位作者 Shu Cai Gan Da Cheng Wang Yu Zhang Xu Ming Deng 《Chinese Chemical Letters》 SCIE CAS 2008年第10期1224-1227,共4页
From the roots ofPicrorhiza scrophulariiflora, two new secoifidoid glycosides, named picrosecosides Ⅰ and Ⅱ （1, 2） have been isolated. Their structures were elucidated on the basis of chemical and spectropic evide... From the roots ofPicrorhiza scrophulariiflora, two new secoifidoid glycosides, named picrosecosides Ⅰ and Ⅱ （1, 2） have been isolated. Their structures were elucidated on the basis of chemical and spectropic evidences. 展开更多
关键词 Picrorhiza scrophulariiflora Secoiridoid glycosides Picrosecosides Ⅰ and Ⅱ
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Simultaneous determination of five phenylethanoid glycosides in Cistanches Herba using quantitative analysis of multi-components by single marker 认领 引用 被引量：11
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作者 Ruiyan Li Mingbo Zhao +1 位作者 Pengfei Tu Yong Jiang 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2019年第8期537-546,共10页
In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside... In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside, isoacteoside, and2’-acetylacteoside in Cistanches Herba. ECH was used as the internal standard(IS) to obtain the relative correction factors(RCFs) of the other four phenylethanoid glycosides(PhGs);meanwhile, various influencing factors on RCFs were investigated under different conditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method(ESM) to verify the feasibility and accuracy of the established QAMS method. No significant difference was found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM. The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba. 展开更多
关键词 Cistanches Herba Phenylethanoid glycosides Quantitative analysis of multi-components by single-marker Relative correction factors
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Two New Flavonoid Glycosides from Chrysanthemum morifolium 认领 引用 被引量：14
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作者 Jian ZHANG An Wei DING +3 位作者 You Bin LI Da Wei QIAN Jin Ao DUAN Zhi Qi YIN 《Chinese Chemical Letters》 SCIE CAS 2006年第8期1051-1053,共3页
Two new flavonoid glycosides were isolated from the flowering heads of Chrysanthemum morifolium. Their structures were determined to be luteolin 4＇-methoxy-7- O-（6＂-O-acetyl）-β-D-glucopyranoside （1） and acaceti... Two new flavonoid glycosides were isolated from the flowering heads of Chrysanthemum morifolium. Their structures were determined to be luteolin 4＇-methoxy-7- O-（6＂-O-acetyl）-β-D-glucopyranoside （1） and acacetin 7-O-（3＂-O-acetyl）-β-D-glucopyranoside （2） by means of 1H and 13C NMR spectroscopic analysis, including 2D NMR technique. 展开更多
关键词 Chrysanthemum morifolium flavonoid glycosides luteolin 4＇-methoxy-7-O-（6＂-O-acetyl）-β-D-glucopyranoside acacetin 7-O-（3＂-O-acetyl）-β-D-glucopyranoside.
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Phenylethanoid glycosides from traditional Mongolian medicine Cymbaria daurica alleviate alloxan-induced INS-1 cells oxidative stress and apoptosis 认领 引用 被引量：6
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作者 Ruyu Shi Xing Li +2 位作者 Bing Gao Chunhong Zhang Minhui Li 《Food Science and Human Wellness》 SCIE CAS CSCD 2023年第5期1580-1589,共10页
Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetesrelated conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed t... Cymbaria daurica L. is a well-known traditional Mongolian medicine, which has been used to treat diabetesrelated conditions characterized by persistent thirst and hunger, copious urination, and weight loss. We aimed to investigate the protective effects of C. daurica extracts and phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. We discovered phenylethanoid glycosides from n-butanol extract with large content through extraction and separation. We continue to study the protective effects of phenylethanoid glycosides including verbascoside and isoacteoside on INS-1 cells. INS-1 cells were treated with C. daurica, cell viability assay, RNA-seq technology, superoxide dismutase activity and malonaldehyde content, quantitative real time-PCR and Western blot analysis were used to study the protective effects of C. daurica. Cell viability assay resulted that n-butanol extract and verbascoside, isoacteoside showed protective effects of C. daurica. According to the RNA-seq technology to identify the differentially expressed genes in INS-1 cells, the pathway of gene enrich the protective effect of C. daurica on oxidative stress. SOD activity and the content of MDA indicated that C. daurica could enhance the antioxidant capacity of INS-1 cells. Further investigation indicated C. daurica alleviate oxidative stress by inhibiting INS-1 cell apoptosis. C. daurica may play an anti-diabetic role by inhibiting islet cell apoptosis. 展开更多
关键词 Diabetes mellitus Cymbaria daurica L. Phenylethanoid glycosides Alloxan-induced cell injury
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Minor antifungal aromatic glycosides from the roots of Gentiana rigescens(Gentianaceae) 认领 引用 被引量：12
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作者 Min Xu Chong Ren Yang Ying Jun Zhang 《Chinese Chemical Letters》 SCIE CAS 2009年第10期1215-1217,共3页
Two new phenolic glycosides,2,3-dihydroxybenzoic acid methyl ester 3-O-β-o-glucopyranosyl-(1-6)-β-D-glucopyranoside(1)and 2,5-dihydroxylbenzofuran 5-O-β-D-xylopyranosyl-(1-6)-O-β-D-glucopyranoside(2),were isolated... Two new phenolic glycosides,2,3-dihydroxybenzoic acid methyl ester 3-O-β-o-glucopyranosyl-(1-6)-β-D-glucopyranoside(1)and 2,5-dihydroxylbenzofuran 5-O-β-D-xylopyranosyl-(1-6)-O-β-D-glucopyranoside(2),were isolated as the minor chemical constituents from the roots of Gentiana rigescens,along with 15 known compounds.Their structures were elucidated by detailed spectroscopic analysis,including 1D,2D NMR and chemical method.All of these compounds were isolated for the first time from the title plant.Moreover,compounds 1 and 2 were tested for the antifungal activities on three plant pathogens Peronophythora litchi,Glomerella cingulata,and Glorosprium musarum. 展开更多
关键词 Gentiana rigescen Phenolic glycosides Anfifungal activity Plant pathogen
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Three new bioactive flavonoid glycosides from Viscum album 认领 引用 被引量：3
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作者 DAI Jia-Kun CAO Duo +6 位作者 LI Cui-Hua GAO Jing LI Meng-Qing FAN Na WEI Ya-Hui SUN Zheng-Liang HOU Meng-Yang 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2019年第7期545-550,共6页
Two new flavonoid glycosides, named viscumneoside XⅡ(1), and viscumneoside XⅢ(2);a new dihydrogen flavonoid glycoside product named viscumneoside XⅣ(3), were isolated from the aerial part of Viscum album, along wit... Two new flavonoid glycosides, named viscumneoside XⅡ(1), and viscumneoside XⅢ(2);a new dihydrogen flavonoid glycoside product named viscumneoside XⅣ(3), were isolated from the aerial part of Viscum album, along with seven known compounds(4-10). Their structures were identified by analysis of spectroscopic data. In addition, cytotoxicity assay showed that 1, 2 and 3 possessed significant inhibitory activities against C6, A549 and MDA-MB-231(the inhibition rate arrived about 50%, 70% and74% respectively with IC50 ≤ 60.00 μmol·L^-1), while the inhibition of TF-1 and Hela was not significant. 展开更多
关键词 Viscum album Flavonoid glycosides Cytotoxic activities
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