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Representative appressorium stage cDNA library of Magnaporthe grisea 认领 引用 被引量：12
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作者 卢建平 刘同宝 +1 位作者 于晓云 林福呈 《Journal of Zhejiang University-SCIENCE B》 EI CAS 2005年第2期132-136,共5页
A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a λTriplEx2 vector by SMART?cDNA library containing 2.37×106 independent clones about 100% of which harbor foreign ... A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a λTriplEx2 vector by SMART?cDNA library containing 2.37×106 independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M grisea. 展开更多
关键词 Magnaporthe grisea Appressorium cDNA library
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Race Specificity of Major Rice Blast Resistance Genes to Magnaporthe grisea Isolates Collected from indica Rice in Guangdong, China 认领 引用 被引量：3
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作者 Jian-yuan YANG Shen CHEN +4 位作者 Lie-xian ZENG Yi-long LI Zhen CHEN Chuan-ying LI Xiao-yuan ZHU 《Rice science》 2008年第4期311-318,共8页
Race-specific resistance and field resistance of 30 rice blast resistance monogenic lines derived from different resources were evaluated. The spectra of resistance to 163 Magnaporthe grisea isolates collected from in... Race-specific resistance and field resistance of 30 rice blast resistance monogenic lines derived from different resources were evaluated. The spectra of resistance to 163 Magnaporthe grisea isolates collected from indica rice in Guangdong Province, China ranged from 0.6% to 89.6%. Most of the monogenic lines showed a narrow resistance spectrum and high susceptibility in rice blast area, whereas the lines with Pikh and Pi1(t) had the broad resistance spectra of 89.6% and 82.2% respectively, showing a high and stable blast resistance in fields. According to the cluster analysis of specific resistance to 163 blast isolates tested, the 30 monogenic lines were divided into 15 groups, and based on the principal factor analysis, nine kinds of race-specific resistance were identified. Pik, Piz5, Pi9 and Pish can be used as candidate resistance genes for rice breeding since their specific resistance differed from those of the backbone parents in Guangdong, China. Gene pyramiding of Pikh [or Pi1(t)], Pi9 (or Piz5) and Pish (or Pita2) will be effective to obtain broad-spectrum blast resistance in rice breeding program in Guangdong, China. The strategies for studying and application of rice blast resistance genes were discussed. 展开更多
关键词 rice race-specific resistance rice blast Magnaporthe grisea field resistance resistance gene
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Expression of a Magnaporthe grisea Elicitor and Its Biological Function in Activating Resistance in Rice 认领 引用 被引量：3
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作者 YAO Qing YANG Xiu-fen +4 位作者 LIANG Ying Xu Feng LIu Zheng YUAN Jing-jing QIU De-wen 《Rice science》 2007年第2期149-156,共8页
The expression of a protein elicitor from Magnaporthe griesea and its biological function in activating resistance in rice （Oryza safiva L） were reported. The gene of elicitor was expressed in Escherichia colicells ... The expression of a protein elicitor from Magnaporthe griesea and its biological function in activating resistance in rice （Oryza safiva L） were reported. The gene of elicitor was expressed in Escherichia colicells and produced a His6-fusion protein with 42 kD apparent molecular weight on SDS-PAGE. The purified protein could induce the resistance to blast disease, with the control efficiency of 46.47% and 36.41% at the 14^th day and the 21^st day after blast inoculation, respectively. After treatment with the expressed protein, the phenylalanine ammonia-lyase （PAL） and peroxidase （POD） activities were promoted in rice plants, meanwhile, the transcription levels of STKM, FAD, PBZ1 and PR1 genes were increased in rice plants. Moreover, after comparing the profile of total rice leaf proteins on two-dimensional electrophoresis gel, about 14 proteins were found to be increased in expression level after the expressed protein treatment. All the results indicated that the expressed protein could act as an elicitor to trigger the resistance in rice. 展开更多
关键词 elicitor Magnaporthe grisea induced resistance protein expression blast resistance rice biologica l control
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Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea 认领 引用 被引量：5
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作者 JIN Qing-chao DONG Hai-tao +8 位作者 PENG You-liang CHEN Bao-shan SHAO Jing DENG Ye DAI Cheng-en FANG Yong-qi LOU Yi-chun LI You-zhi LI De-bao 《Journal of Zhejiang University-SCIENCE B》 EI CAS 2007年第2期88-97,共10页
Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene expression profiles ofappressorium development provides insight into the molecular basis of pathogenicity and c... Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene expression profiles ofappressorium development provides insight into the molecular basis of pathogenicity and control of this fungal plant disease. A cDNA array representing 2927 unique genes based on a large EST （expressed sequence tag） database ofM. grisea strain Y34 was constructed and used to profile the gene expression patterns at mycelium and appressorium maturation stages. Compared with mycelia, 55 up-regulated and 22 down-regulated genes were identified in mature appressoria. Among 77 genes, 16 genes showed no similarity to the genome sequences of M. grisea. A novel homologue of peptidyl-prolyl cis-trans isomerase was found to be expressed at low-level in mature appressoria of M. grisea. The results indicated that the genes such as pyruvate carboxylase, phospholipid metabolism-related protein and glyceraldehyde 3-phosphate dehydrogenase involved in gluconeogenesis, lipid metabolism and glycolysis, showed differential expression in mature appressoria. Furthermore, genes such as PTHll, beta subunit of G protein and SGTI involved in cell signalling, were expressed differentially in mature appressoria. Northern blot analysis was used to confirm the cDNA array results. 展开更多
关键词 Magnaporthe grisea Mature appressoria cDNA array Gene expression profile
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Cloning,sequencing and expression analysis of the NAR promoter activated during hyphal stage of Magnaporthe grisea 认领 引用 被引量：2
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作者 LU Jian-ping DUAN Zhi-bing +1 位作者 LIU Tong-bao LIN Fu-cheng 《Journal of Zhejiang University-SCIENCE B》 EI CAS 2007年第9期661-665,共5页
The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. Program... The promoter of NAR gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. Programs that predict promoter sequences in-dicated that promoter sequence lies between locations 430 and 857 of the NAR promoter fragment. GFP expression under the NAR promoter and NAR transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus. 展开更多
关键词 Magnaporthe grisea Promoter NAR Clone Hypha
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Sequence analysis and expression pattern of MGTA1 gene in rice blast pathogen Magnaporthe grisea 认领 引用 被引量：3
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作者 王教瑜 刘小红 +1 位作者 卢建平 林福呈 《Journal of Zhejiang University-SCIENCE B》 EI CAS 2005年第8期817-824,共8页
MGTA1, a putative fungal Zn（Ⅱ）2Cys6 transcriptional activator-encoding gene, was isolated from rice blast pathogen Magnaporthe grisea, which is homologous to CLTA1 from Colletotrichum lindemuthianum with 51% identi... MGTA1, a putative fungal Zn（Ⅱ）2Cys6 transcriptional activator-encoding gene, was isolated from rice blast pathogen Magnaporthe grisea, which is homologous to CLTA1 from Colletotrichum lindemuthianum with 51% identity at protein level. MGTA1 cassette contains a 2370 bp open reading frame, consisting of 6 exons, coding a 790 amino acid peptide. MGTA1 gene exists as a single copy in genomes of 7 strains of M. grisea, and is expressed in tip hyphae, conidia, and mature appressoria of strain Guy 11. 展开更多
关键词 Magnaporthe grisea MGTA1 gene Zn（Ⅱ）2Cys6
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Promoter trapping in Magnaporthe grisea 认领 引用 被引量：2
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作者 刘小红 卢建平 +2 位作者 王教瑜 闵航 林福呈 《Journal of Zhejiang University-SCIENCE B》 EI CAS 2006年第1期28-33,共6页
Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protopl... Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis. 展开更多
关键词 Promoter trapping Green fluorescent protein Magnaporthe grisea
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Analysis of the Abnormal Segregation of Pathogenicity in Magnaporthe grisea by Using a Genetic Cross of Oryza and Eleusine Isolates 认领 引用 被引量：3
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作者 YIN Liang-fen LUO Chao-xi +1 位作者 Kusaba Motoaki Yaegashi Hiroshi 《Agricultural Sciences in China》 2010年第3期383-391,共9页
A genetic cross between Oryza isolate Y93-164a-1 and Eleusine isolate SA98-4 was established, and the pathogenicity of 151 F1 progeny isolates was investigated on both host plants rice and finger millet. Results showe... A genetic cross between Oryza isolate Y93-164a-1 and Eleusine isolate SA98-4 was established, and the pathogenicity of 151 F1 progeny isolates was investigated on both host plants rice and finger millet. Results showed that the segregation of pathogenicity in this genetic cross was abnormal, i.e., most of the progeny isolates were nonpathogenic on both host plants. However, no abnormal segregation was observed when middle repetitive sequence MGR586 and 31 single-copy RFLP markers from all of the chromosomes were genetically analyzed. At the same time, comparison of the chromosomal organization among two pairs of parental isolates did not find any genomic abnormity. These results suggested that the ＂abnormal＂ inheritance of pathogenicity in this cross was most likely due to the reassortment of numerous host species specificity genes but not the biased segregation of the host species specificity genes. The host species specificities in M. grisea were likely to be multigenically controlled, at least in the genetic cross involving rice pathogen and the grasses pathogen other than rice. 展开更多
关键词 Magnaporthe grisea rice blast fungus host species specificity pathogenicity contour-clamped homogeneous electric field （CHEF） electrophoresis Southern hybridization
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Antifungal Effect of Streptomyces 702 Antifungal Monomer Component DZP8 on Rhizoctonia solani and Magnaporthe grisea 认领 引用 被引量：2
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作者 芦慧 涂晓嵘 +2 位作者 徐佳 冯华根 涂国全 《Agricultural Science & Technology》 CAS 2012年第11期2370-2374,2378,共5页
The aim of this study was to investigate the in vitro antifungal effects of antifungal monomer component DZP8 isolated from Streptomyces 702 on the mycelium growth, sclerotium formation and germination of Rhizoctonia ... The aim of this study was to investigate the in vitro antifungal effects of antifungal monomer component DZP8 isolated from Streptomyces 702 on the mycelium growth, sclerotium formation and germination of Rhizoctonia solani and on the mycelium growth, conidial formation, germination, appressorium formation of Magnaporthe grisea. The results showed that the antifungal monomer component DZP8 has strong antifungal effect on both the R. solani and M. grisea. The EC50 and EC90 of DZP8 were 1.81 and 3.35 μg/ml on Ft. solani respectively, and 37.01 and 136.21 μg/ml on M. grisea respectively. Under the treatment of 48.01 μg/ml DZP8, the sclerotium formation rate of R. solani was just 39.21%, the formation time delayed by 216 h and the dry weight decreased by 81.37% in comparison the con- trol; and 33.51 μg/ml DZP8 significantly inhibited the sclerotium germination. In the presence of 160.08 μg/ml DZP8, the sporulation of M. grisea was just 9.29% of control sample; 20.14 μg/ml DZP8 inhibited the conidial germination suppression rate by 95.16%, and the appressorium formation by 100%. 展开更多
关键词 Streptomyces 702 Monomer component DZP8 Rhizoctonia solani Mag-naporthe grisea Antifungal effect
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Identification of a Lectin Gene Induced in Rice in Response to Magnaporthe grisea Infection 认领 引用 被引量：12
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作者 秦庆明 张全 +2 位作者 赵文生 王云月 彭友良 《Acta Botanica Sinica》 2003年第1期76-81,共6页
By mRNA differential display, eight induced cDNAs were obtained from rice leaves infected with an incompatible race 131 of Magnaporthe grisea, and one of these cDNAs was highly similar to salt-induced mannose-binding ... By mRNA differential display, eight induced cDNAs were obtained from rice leaves infected with an incompatible race 131 of Magnaporthe grisea, and one of these cDNAs was highly similar to salt-induced mannose-binding lectin gene. Using this fragment as a probe, a full length cDNA was isolated from a nice cDNA library, which was constructed using mRNA from the incompatible race-infected leaves. Sequence analysis indicates that the cDNA encodes a protein of 15 kD with 145 amino, acids and shares 96% identity at nucleotide level with MRL and salT, but is identical to MRL at amino acid level. Genomic Southern blotting shows that there are two mannose-binding lectin genes in rice genome. Northern blotting analysis indicates that the gene was strongly and specifically induced in rice leaves infected with the incompatible race, suggesting that the lectin induction be involved in the defense of rice to M. grisea. 展开更多
关键词 rice mRNA differential display lectin Magnaporthe grisea
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Pathotype Structure of Magnaporthe grisea in the Fields of Chongyang and Yuan'an in Hubei Province,China 认领 引用 被引量：1
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作者 YANG Xiao-lin ZHANG Shu +3 位作者 LU Liang CHANG Xiang-qian YUAN Bing YU Da-zhao 《Agricultural Science & Technology》 CAS 2011年第12期1889-1892,共4页
[Objective] This study aimed to explore the pathotype structure of Magnaporthe grisea in Chongyang and Yuan'an in Hubei Province,China.[Method] From the rice-growing fields of Chongyang and Yuan'an in Hubei Pr... [Objective] This study aimed to explore the pathotype structure of Magnaporthe grisea in Chongyang and Yuan'an in Hubei Province,China.[Method] From the rice-growing fields of Chongyang and Yuan'an in Hubei Province where rice blast occurs frequently,60 isolates which were pathotyped against two sets of host differentials:Chinese host differentials and CO39 NILs,were obtained.Then,20 pathotypes with the six indica host differentials(CO39 NILs) were observed,while 13 pathotypes in four race groups were observed out of the same single spore isolates with Chinese host differentials which consists of three indica cultivars and four japonica cultivars.The diversity of the pathotypes of M.grisea populations tested by CO39 NILs was 2.54 and the pathotype 137.1 occurred at predominantly high frequency(21.67%).The diversity of physiological races of M.grisea populations tested by Chinese host differentials was 1.22 and the race group ZA occurred at predominantly high frequency(73.33%).The diversity of physiological races of M.grisea in Chongyang and Yuan'an were also calculated.Overall,the diversity of pathotypes of M.grisea in Yuan'an was higher than that in Chongyang with the two sets of the host differentials.[Conclusion] This study provided current information on the pathotype spectrum of M.grisea populations in the rice fields of Hubei Province to allow the formulation of viable strategies for blast resistance breeding programs in Hubei Province. 展开更多
关键词 Host differentials Magnaporthe grisea Resistance gene Rice blast
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Polymorphism of microsatellite sequence within signal transduction coding genes in phytopathogenic fungus, Magnaporthe grisea 认领 引用
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作者 LIU Lin LI Cheng-yun +4 位作者 YANG Jing LI Jin-bin SU Yuan WANG Yun-yue ZHU You-yong 《Journal of Life Sciences》 2007年第1期18-21,共4页
Sixteen polymorphic microsatellite markers suitable for population genetic structure analysis and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymo... Sixteen polymorphic microsatellite markers suitable for population genetic structure analysis and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymorphism was evaluated by using forty-six isolates collected from diverse geographical locations （including japonica grown zone, indica grown zone） and rice varieties. Preliminary results indicated that each locus resolved multiple alleles ranging from three to fourteen. The results showed that these SSR-containing genes are also polymorphic in the nature population. 展开更多
关键词 Magnaporthe grisea signal transduction microsatellite
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Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea 认领 引用
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作者 ZHENG Wu CHEN Ji-sheng ZHENG Shi-qin LU Guo-dong WANG Zong-hua 《Agricultural Sciences in China》 2006年第10期780-786,共7页
MgCdc42 （Cdc42 in Magnaporthe grisea）, with high homology to ScCdc42 （Cdc42 in Saccharomyces cerevisiae）, has been demonstrated to involve in the morphogenesis and infection process. To further understand the sign... MgCdc42 （Cdc42 in Magnaporthe grisea）, with high homology to ScCdc42 （Cdc42 in Saccharomyces cerevisiae）, has been demonstrated to involve in the morphogenesis and infection process. To further understand the signaling network, the putative MgCdc42-interacting proteins were analyzed. ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semiquantitative RT-PCR. All retrieved analogs of ScCdc42-interacting proteins from the M. grisea database have conserved domains as those in S. cerevisiae. Expression of their encoding genes increased in MgCdc42CA mutant and decreased in MgCdc42KO mutant. However, MgBeml, Chin1, and MgGicl in MgCdc42DN mutant had the same expression level as that in the wild type, although MgBem4, MgBoi2, MgCdc24, MgGic2, MgRgal, and Mst20 had decreased expression level, as expected. Overall, it is concluded that there may exist a similar Cdc42 signal pathway in M. grisea as in S. cerevisiae and MgCdc42 plays a key role in the pathway. 展开更多
关键词 Cdc42 Magnaporthe grisea guanine nucleotide exchange factor GTPase-activating protein
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A New p-Terphenyl Derivative from Basidiomycetes Boletopsis grisea 认领 引用 被引量：3
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作者 Lin HU Ze Jun DON Ji Kai LIU 《Chinese Chemical Letters》 SCIE CAS 2001年第4期335-336,共2页
A new p-terphenyl derivative 1 was isolated from the fruiting bodies of Boletopsis grisea. Its structure was established as 2. 3-diacetoxy-4 ', 4 ', 5, 6-tetrahydroxy-p-terphenyl by spectroscopic and chemical ... A new p-terphenyl derivative 1 was isolated from the fruiting bodies of Boletopsis grisea. Its structure was established as 2. 3-diacetoxy-4 ', 4 ', 5, 6-tetrahydroxy-p-terphenyl by spectroscopic and chemical means. 展开更多
关键词 Boletopsis grisea p-terphenyl derivative Basidiomycetes.
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Efficacy of Selected Plant Extracts against Pyricularia grisea, Causal Agent of Rice Blast Disease 认领 引用 被引量：7
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作者 Judith Hubert Robert B. Mabagala Delphina P. Mamiro 《American Journal of Plant Sciences》 CAS 2015年第5期602-611,共10页
Rice blast disease, caused by a seed-borne fungus Pyricularia grisea, is an important and serious disease of rice (Oryza sativa L.) worldwide. The disease has been reported to cause yield losses of up to 40% in Tanzan... Rice blast disease, caused by a seed-borne fungus Pyricularia grisea, is an important and serious disease of rice (Oryza sativa L.) worldwide. The disease has been reported to cause yield losses of up to 40% in Tanzania. Studies were conducted to determine the effect of aqueous extracts of Aloe vera, Allium sativum, Annona muricata, Azadirachta indica, Bidens pilosa, Camellia sinensis, Chrysanthemum coccineum, processed Coffee arabica, Datura stramonium, Nicotiana tabacum and Zingiber officinalis for control of rice blast disease (Pyricularia grisea) in-vitro and in-vivo. The results indicate that processed C. arabica at 10% and 25% (v/v) had the highest (81.12%) and (89.40%) inhibitory effect, respectively, against P. grisea. Aqueous extract from N. tabacum at 10% concentration ranked third (80.35%) in inhibiting P. grisea. These were followed by extracts from 25% A. vera (79.45%) and 25% C. coccineum flower (78.83%). The results also indicate that, extracts from A. indica, A. vera, A. sativum, C. arabica, D. stramonium, C. sinensis, Z. officinalis and N. tabacum did not have any phytotoxic effect on seed germination, shoot height, root length, dry weight, seedling growth and seedling vigour index. These plant extracts can thus be used for rice seed treatment to manage rice blast disease. 展开更多
关键词 Plant Extracts Pyricularia grisea Rice
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Geographic Distribution of Mating Types in Magnaporthe grisea and the Relationship Between Fertile Isolates in China 认领 引用 被引量：5
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作者 SHEN Ying, Notteghem Jean Loup., Milazzo Joelle., YUAN Xiao-ping, Adreit Henry, ZHAO Xin-hua, WANG Yan-li and Tharreau Didier.(China National Rice Research Institute, Hangzhou 310006, P. R. China National Institute of Agronomic Research ,Unit of Training and Biological Research , Ecology and Plant Pathology, 2 Place Viala , 34062 Montpellier , France International Center for collaboration in Agronomic Research and Development,TA 73 /09 , 34398 Montpellier , Cedex 05 , France) 《Agricultural Sciences in China》 2002年第6期648-656,共9页
377 isolates of Magnaporthe grisea were collected from 17 provinces in China and their geographic distribution of mating types and their fertility was tested with four standard isolates, KA3 and TH12 (Mat1.1) and Guy1... 377 isolates of Magnaporthe grisea were collected from 17 provinces in China and their geographic distribution of mating types and their fertility was tested with four standard isolates, KA3 and TH12 (Mat1.1) and Guy11 and TH16 (Mat1.2) provided by CIRAD. 73 fertile isolates were tested with SCAR markers of 13 pairs of primers. Preliminary results showed that the geographic distribution of M.grisea existed among isolates collected from the same location as well as different locations and the genetic relationship between fertile isolates of the fungus in China. The existence of sexual reproduction of M .grisea was explored in the field as well. 展开更多
关键词 Magnaporthe grisea Fertile isolates Geographic Distribution SCARs Genetic relationship
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Physiological Macro-lesions Enhanced Resistance to Blast (Magnaporthe grisea) in Rice Near-isogenic Lines 认领 引用 被引量：1
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作者 HUANG Shi-wen Lu Ji-ying +2 位作者 Luo Kun ZHANG Xiu-fu QIAN Qian 《Rice science》 2005年第2期148-150,共3页
Roll-leaf-1 （rl-1） and spot-leaf-1 （spl-1） were two near-isogenic lines, which were obtained after 3 to 4 backcrosses with early season indica rice Zhefu 802 as recurrent parent. Henna macro-lesions, referred as p... Roll-leaf-1 （rl-1） and spot-leaf-1 （spl-1） were two near-isogenic lines, which were obtained after 3 to 4 backcrosses with early season indica rice Zhefu 802 as recurrent parent. Henna macro-lesions, referred as physiological or morphological markers, began to appear on leaves at 4.5- to 6.0-leaf stage. The rice seedlings were inoculated at 3.5-, 5.0- and 7.0-leaf stages with high pathogenic races Zhong A1 and Zhong B1 of Magnaporthe grisea, respectively. The resistance of rl-1, spl-1 and Zhefu 802 against blast was significantly different. The seedlings of Zhefu 802 at 3.5- to 7.0-leaf stage were susceptible to races Zhong A1 and Zhong B1 of M. grisea, whereas those of rl-1 and spl-1 at 3.5-, 5.0- and 7.0-leaf stages were susceptible, moderately resistant and resistant, respectively. These results suggested that the enhanced resistance of rl-1 and spl-1 related to the appearance of their morphological marker lesions. The experiment provided a basis for studying lesion mimic and hypersensitive response in association with disease resistance. 展开更多
关键词 rice near-isogenic lines Magnaporthe grisea resistance
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Factors affecting appresorium formation of Magnaporthe grisea 认领 引用 被引量：1
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作者 WANG Hongkai LIN Fucheng LI Debao Biotechnologv Inst,Zhe- jiang Univ Hangzhou 310029,China 《Chinese Rice Research Newsletter》 2001年第4期6-7,共2页
M. grisea causes one of the major diseases on rice, rice blast. Appresoriumformation is the key for the pathogen to penetrate into host tissue. So, it is im-portant to study the effect of environmental factors on the ... M. grisea causes one of the major diseases on rice, rice blast. Appresoriumformation is the key for the pathogen to penetrate into host tissue. So, it is im-portant to study the effect of environmental factors on the appresorinm formation 展开更多
关键词 IBMX Factors affecting appresorium formation of Magnaporthe grisea rate
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Vegetative Compatibility and Asexual Recombination in Magnaporthe grisea from Jiangsu Province, China 认领 引用
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作者 WANG Yan-li, ZHANG Zheng-guang and ZHENG Xiao-bo 《Agricultural Sciences in China》 2003年第7期768-772,共5页
Vegetative compatibility among isolates of different races in Magnaporthe grisea collected from Jiangsu Province and asexual recombination among compatible isolates by anastomosis were tested. Twenty isolates involvin... Vegetative compatibility among isolates of different races in Magnaporthe grisea collected from Jiangsu Province and asexual recombination among compatible isolates by anastomosis were tested. Twenty isolates involving seven races from diseased rice plants were paired on polished rice rose bengal medium and incubated at 25℃ in darkness for 18 days. Among 173 pairings tested, solid hyphal fusion lines formed by anastomosis between 124 pairings, indicated that these isolates were vegetative compatible with each other. The result showed that most M. grisea isolates were vegetative compatible. Furthermore, 17 vegetative compatible pairings between monoconidial isolates with MBCsIPTr marker and isolates with MBCrIPTs marker were selected to detect the asexual recombination between the compatible isolates of different races. The asexual recombinants with MBCrIPTr marker were detected in single hyphal fragment progenies in thirteen of the seventeen pairings. The percentage of recombinants was about 0. 6 -11.3%. Results showed that vegetative compatibility was prevailing among isolates of M. grisea in Jiangsu Province in vitro. These results also suggested that asexual recombination may be an important mechanism for M. grisea to maintain genetic diversity in nature. 展开更多
关键词 Magnaporthe grisea Vegetative compatibility Asexual recombination
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Sources of Resistance to Blast Disease(Pyricularia grisea L.)in Finger Millet(Eleusine Coracana)Germplasm 认领 引用
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作者 C.M.Wekesa P.K.Kimurto +5 位作者 C.O.Oduori B.K.Towett L.Jeptanui H.Ojulong E.Manyasa M.Siambi 《Journal of Life Sciences》 2019年第1期34-47,共14页
Blast has been a continuous threat to finger millet production in semi-arid tropics.The disease is economically important and widespread in finger millet major growing areas of western,Nyanza and Rift valley in Kenya.... Blast has been a continuous threat to finger millet production in semi-arid tropics.The disease is economically important and widespread in finger millet major growing areas of western,Nyanza and Rift valley in Kenya.Host resistance is the most economical and effective means of controlling the disease as finger millet is grown by resource-poor farmers.This study evaluated a hundred finger millet genotypes for resistance to blast at KALRO(Kenya Agricultural Research and Livestock Organization)Kakamega and Alupe,Kenya for two seasons in 2011/2012 in a lattice square design for two seasons each.Data on blast incidence and severity were measured.Data were analyzed using ANOVA(analysis of variance)and means separated using Duncan multiple range test(DMRT)at p<0.005.The results showed that disease severity was highest in early maturing varieties and lowest in the late maturing varieties.The most resistant genotypes were:GBK000702,GBK000513,GBK029869,GULU-E,GBK000752 and Busibwabo.Pearson correlation analysis between neck severity and physiological maturity was positively significant(r=-0.47).A strong positive correlation between finger severity and neck severity(r=0.87)was observed.These tolerant genotypes could be utilized as donor parents for breeding durable blast resistant varieties. 展开更多
关键词 Eleusine coracana incidence Pyricularia grisea severity mid altitude zones
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