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Transcriptome analysis of genes and pathways associated with growth performance in eyestalks of Exopalaemon carinicauda under different light intensities 认领 引用
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作者 Guifang JIA Chengsong ZHANG Fuhua LI 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2026年第3期1253-1266,共14页
Light is one of the important environmental factors on life activities of aquatic animals.Eyestalks are a multifunctional organ of crustaceans that conducts light signals,produces neurohormones,and regulates growth.Ho... Light is one of the important environmental factors on life activities of aquatic animals.Eyestalks are a multifunctional organ of crustaceans that conducts light signals,produces neurohormones,and regulates growth.However,the mechanism of growth regulation by light intensity remains poorly studied.We evaluated the growth performance of ridgetail white prawn(Exopalaemon carinicauda)under different light intensities(0,100,200,and 400 lx),and determined the regulatory mechanism of growth in response to light intensity through transcriptome analysis of eyestalks.Results show that light intensity at 100 lx significantly improved growth performance in growth rate,specific growth rate,and biomass increase rate,while 0-lx and 400-lx conditions inhibited the growth.A total of 931 DEGs were identified between the 100-lx and 0-lx groups,of which 411 were upregulated and 520 were downregulated.In addition,456 DEGs were identified between groups of 100-lx and 400-lx,including 155 upregulated DEGs and 301 downregulated DEGs.The light intensity at 100 lx significantly improved the growth performance by upregulating the oxidative phosphorylation,Toll and Imd signaling pathway,and ribosome pathway,and downregulating the tyrosine metabolism,phagosome,lysosome,autophagy,and Hippo signaling pathway,whereas 0 lx and 400 lx inhibited growth in the opposite patterns.In total,eight growth-regulation-related candidate genes,i.e.,hemocyanin,NADH dehydrogenase,cytochrome c oxidase,ATP synthase,cathepsin L,phenoloxidase activating factor,CLIP domain-containing serine protease,and 40S/60S ribosomal protein were identified.The optimal light intensity for improved growth of E.carinicauda was about 100 lx when reared indoor.These data shall provide key information for further understanding of the regulatory mechanism of light intensity on the growth performance of this species. 展开更多
关键词 Exopalaemon carinicauda eyestalk growth performance light intensity transcriptome
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Dietary processed former foodstuffs for broilers:impacts on growth performance,digestibility,hematobiochemical profiles and liver gene abundance 认领 引用
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作者 Karthika Srikanthithasan Marta Gariglio +14 位作者 Elena Diaz Vicuna Edoardo Fiorilla Barbara Miniscalco Valeria Zambotto Eleonora Erika Cappone Nadia Stoppani Dominga Soglia Federica Raspa Joana Nery Andrea Giorgino Roser Sala Andrés Luis Martínez Marínz Josefa Madrid Sanchez Achille Schiavone Claudio Forte 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期364-376,共13页
Background The present experiment aimed to evaluate the effects of commercially processed former foodstuffs(cFF)as dietary substitutes of corn,soybean meal and soybean oil on the growth performance,apparent total trac... Background The present experiment aimed to evaluate the effects of commercially processed former foodstuffs(cFF)as dietary substitutes of corn,soybean meal and soybean oil on the growth performance,apparent total tract digestibil-ity(ATTD),hematobiochemical profiles,and liver gene abundance in broiler chickens.Two hundred one-day-old male ROSS-308 chicks were assigned to 4 dietary groups(5 replicates of ten birds per replicate)according to their average body weight(BW,38.0±0.11 g).All groups received a two-phase feeding program:starter,d 1–12 and grower,d 12–33.The control group(cFF0)was fed a standard commercial feed based on corn,soybean meal and soybean oil.The other three groups received diets in which the feed based on corn,soybean meal,and soybean oil was partially replaced with cFF at a substitution level of 6.25%(cFF6.25),12.5%(cFF12.5)or 25%(cFF25)for the following 33 d.Results The growth performance data showed no differences in BW or average daily gain among groups,although the average daily feed intake decreased during the grower period(12–33 d)and over entire experimental period(1–33 d)in a linear manner as the cFF inclusion level rose(P=0.026),positively affecting the gain to feed ratio(P=0.001).The ATTD of dry matter of the cFF-fed groups were greater with respect to control group and increased throughout the experimental period,whereas the ATTD of ether extract linearly decreased with increasing levels of cFF-fed groups compared with control group and throughout the experimental period(P<0.05).Additionally,a lin-ear increase in the heterophil to lymphocyte ratio,serum cholesterol,triglycerides and alanine-aminotransferase were observed with increasing dietary levels of cFF(P<0.05);however,no differences were observed in lipoprotein lipase or sterol regulatory element binding transcription factor gene abundance.Conclusions The results of this experiment demonstrate that it is possible to incorporate cFF into nutritionally balanced diets for broiler chickens,even up to 25%substitution levels,for up to 33 d without adversely impacting the overall growth performance of male broiler chickens raised under commercial conditions.Further studies are essential to validate the hematological trait findings. 展开更多
关键词 Alternative feed Broiler chicken Digestibility Former foodstuff Gene abundance Growth performance
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Growth performance and carcass traits of growing and finishing pigs fed diets with a partial to total replacement of soybean meal with Spirulina powder 认领 引用
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作者 Gregorio Don Diana Giannuzzi +2 位作者 Alessandro Toscano Stefano Schiavon Luigi Gallo 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第4期1718-1731,共14页
Background The protein sources in pig diets strongly rely on soybean meal,but its production has been associated with soil degradation,deforestation and loss of biodiversity.Microalga Spirulina can be a potentially mo... Background The protein sources in pig diets strongly rely on soybean meal,but its production has been associated with soil degradation,deforestation and loss of biodiversity.Microalga Spirulina can be a potentially more sustainable alternative to soybean meal,but comprehensive information about its use in growing pigs is still lacking.This study aimed to evaluate the effects of partial to full replacement of dietary soybean meal with Spirulina on the growth and carcass traits of growing pigs and on the chemical and physical attributes of the meat.Methods Eighty-eight pigs,gilts and barrows mixed together,with initial body weight of 52.4±4.2 kg,were allotted into 4 isoenergetic,isoproteic,and isoaminoacidic dietary treatments,which included a conventional control diet based on cereals and soybean meal and one of 3 diets formulated by replacing nearly 33%,66%or 100%soybean with Spirulina.Each treatment had 2 pens(11 pigs/pen),which were equipped with electronic feeders that were able to record individual feed intake.After 138 d on feed,at 174.9±6.4 kg body weight,the pigs were slaughtered,and the carcass traits and meat quality parameters from loin samples were assessed.Results The palatability of feeds was not depressed in pigs fed Spirulina,even when the soybean was completely replaced by the microalga.The incorporation of Spirulina in the diets in place of soybean did not impair the growth rate or feed efficiency,irrespective of the extent of replacement.The carcass traits and yield of commercial cuts were comparable for all Spirulina-included compared with those of the soybean-based groups,and the same was found for the chemical and physical attributes of loin meat.Conclusion The results obtained at the herd and slaughter levels revealed that the replacement of soybean meal with Spirulina did not negatively affect the growth or carcass traits of growing pigs or the main attributes of meat.Therefore,this study provides,for the first time,insights into the technical possibility of switching growing pig feeding systems toward more environmentally sustainable diets by including a microalga originating from landless feed production systems,which does not result in soil degradation or loss of biodiversity. 展开更多
关键词 Carcass traits Growing-finishing pigs Growth performance Meat quality attributes Novel protein sources Spirulina
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Growth performance and total tract digestibility of nutrients for weanling pigs are improved by an exogenous xylanase and a stimbiotic regardless of maternal xylanase consumption 认领 引用
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作者 Jessica P.Acosta Charmaine D.Espinosa +1 位作者 Gemma González‑Ortiz Hans H.Stein 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2120-2133,共14页
Background Exogenous xylanase can increase utilization of fiber and energy when included in diets for pigs,and xylo-oligosaccharides(XOS)may improve growth performance of pigs by modulating intestinal fermentation.How... Background Exogenous xylanase can increase utilization of fiber and energy when included in diets for pigs,and xylo-oligosaccharides(XOS)may improve growth performance of pigs by modulating intestinal fermentation.However,it is unclear if a stimbiotic(i.e.,a combination of xylanase and XOS)has superior effects compared with a xylanase alone,and there is a lack of data demonstrating if xylanase fed to lactating sows influences growth performance of weanling pigs.Therefore,two hypotheses were tested:1)xylanase and stimbiotic improve growth performance,apparent total tract digestibility(ATTD)of gross energy(GE)and total dietary fiber(TDF),digestible energy(DE),and intestinal health of weanling pigs and 2)offspring of sows fed xylanase in lactation have greater growth performance after weaning than offspring of sows fed no xylanase during lactation.Methods One hundred and twenty pigs were weaned from sows fed a diet without xylanase,and 120 pigs were weaned from sows fed a lactation diet containing 16,000 beechwood xylanase units per kg(initial weight:5.81±0.50 kg).Pigs were allotted to a 2×3 factorial with two sow groups(lactation diet without or with xylanase)and three dietary treatments(i.e.,control,control plus xylanase,or control plus stimbiotic).Results There were no interactions between sow treatment and post-weaning pig treatment,and sow treatment did not impact post-weaning growth or ATTD of GE and TDF in weaned pigs.From d 15 to 28 post-weaning,the ADG,G:F,ATTD of GE and TDF,and concentration of DE were greater(P<0.05)for pigs fed the diet with stimbiotic than if fed the xylanase diet or the control diet,and pigs fed the xylanase diet had greater(P<0.05)ADG,G:F,ATTD of GE and TDF,and concentration of DE than pigs fed the control diet.From d 29 to 42 post-weaning,pigs fed the diets with xylanase or stimbiotic had greater(P<0.05)ADG,ATTD of GE and TDF,and DE than pigs fed the control diet.Conclusions Pigs fed xylanase or stimbiotic had greater ATTD of GE and TDF,greater DE,and greater overall ADG,G:F,and final body weight on d 42 post-weaning than pigs fed the control diet,but feeding sows xylanase in lactation did not influence post-weaning growth performance. 展开更多
关键词 Digestibility Growth performance Sows Stimbiotic Weanling pigs Xylanase
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Effect of Moringa stenopetala and Pigeon Pea Supplements on Growth Performance of Short-Eared Somali Goat Breed 认领 引用
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作者 Mahamed Hassen Abdihakim Ma’alin Abdisayid Mohamed 《Open Journal of Animal Sciences》 2025年第1期69-76,共8页
This study was conducted at Fafan Research Center, Golajo research site to evaluate the effect of Moringa stenopetala and pigeon pea leaf supplementation on growth performance of short-eared Somali goat breed. A total... This study was conducted at Fafan Research Center, Golajo research site to evaluate the effect of Moringa stenopetala and pigeon pea leaf supplementation on growth performance of short-eared Somali goat breed. A total of fifteen yearling indigenous short-eared Somali goat breeds with an initial weight of 15.2 ± 0.30 kg were assigned to three treatment groups using completely randomized design. Pigeon pea (Pp) and Moringa stenopetala (MS) feeds were formulated using 0%, 5%, and 10% inclusion levels of MSLM and PPLM as experimental diets, respectively. The feed of the experiment was prepared in two levels (2 kg of Moringa stenopetala and 2 kg of pigeon pea) and was supplemented to experimental animals in treatments one and two, respectively. The average e initial body weight of selected male goats was 18.82 ± 0.37, 18.8 ± 0.37 and 17.8 ± 0.37 kg under treatment groups T1, T2 and control respectively. Data was analyzed using general linear model (GLM) procedure of SAS computer package Version 9.0 (SAS, 2002). The final weights gain (FWG) of goats on T1 and T2 of experimental group was significantly (P 0.05) the final weight gain of goat supplemented on Moringa stenopetala (T1) and pigeon pea levels (T2). The mean average weight gains (AWG) obtained from the supplemented group in this study were 7.50 ± 0.37 and 7.82 ± 0.37 kg for T1 and T2, whereas mean weight gains for un-supplemented goats were found to be 6.26 ± 0.37 kg. Feeding of dried Moringa stenopetala and pigeon pea leaves mixture improved body weights and average daily body weight gain without affecting feed intake and overall health of Somali goat breed. As Moringa stenopetala and pigeon pea leaves are rich nitrogen/protein source, they can be used effectively as substitute for conventional concentrate in the diet of growing goats at small holder farmer’s level where they can be grown in abundance. Therefore, for higher quality of forage and higher total DM yield for animal feeding, moringa should be harvested at wider harvesting intervals of at least 6th- to 8th-week intervals. Similarly, for pigeon peas, 4- to 6-week harvesting interval can result in optimum forage as well as feed quality and resulted in better growth performances for Somali short-eared goat breeds. 展开更多
关键词 Effect Growth Performance Moringa Pigeon Pea Supplementation Somali Goat
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Effects of Substituting Corn Pellets with Potato Residue on Growth Performance,Rumen Fermentation Indicators,and Economic Benefits of Xinjiang Brown Cattle 认领 引用
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作者 Tian Yingqiao Zhao Yan +2 位作者 Zhang Xinyu Zhang Ruiying Zhang Hao 《Animal Husbandry and Feed Science》 CAS 2025年第1期14-21,共8页
[Objective]The paper was to investigate the effects of substituting corn pellets with potato residue on the growth performance,rumen fermentation indicators and economic benefits of Xinjiang Brown cattle.[Method]A tot... [Objective]The paper was to investigate the effects of substituting corn pellets with potato residue on the growth performance,rumen fermentation indicators and economic benefits of Xinjiang Brown cattle.[Method]A total of 80 Xinjiang Brown cattle,each 22 months old and with a similar body weight of(250±15)kg,were randomly assigned to four groups,with 20 cattle in each group,utilizing a single-factor experimental design.The total mixed diets for the experimental groups Ⅰ,Ⅱ,and Ⅲ were formulated by substituting corn pellets with 2.00%,4.00%,and 6.00%potato residue,respectively,while the diet in the control group was not substituted with potato residue.The duration of the test was 100 d,which comprised a pre-test period of 10 d and a formal test period of 90 d.During the test period,the average dry matter intake,average daily weight gain and feed-gain ratio were assessed at 30-day intervals,and body size indicators were measured on days 0 and 90.Rumen fluid samples were collected from the oral cavity on days 0,30,60,and 90 to assess rumen fermentation parameters.Additionally,jugular blood samples were obtained during the fasting period,and serum was separated for the analysis of total protein mass concentration,urea nitrogen,and glucose concentration.Furthermore,the economic benefits accrued during the test period were calculated.[Result]The dry matter intake in experimental group Ⅰ was significantly higher than that in the control group from days 31 to 60(P0.05).From days 61 to 90,the dry matter intake in experimental group Ⅰ remained significantly higher than that in the other groups(P0.05).On the 90th day of the experiment,the serum urea nitrogen concentration in beef cattle from experimental groups Ⅰ and Ⅱ was significantly lower than that in the control group and experimental group Ⅲ(P<0.05).Additionally,the profit margins in experimental groups Ⅰ,Ⅱ,and Ⅲ were higher than those in the control group,and the profit margin in experimental group Ⅰ was the highest.[Conclusion]The substitution of corn pellets with potato residue has the potential to enhance the average daily weight gain and total weight gain throughout the entire period in beef cattle,while simultaneously reducing the feed-gain ratio.However,this substitution does not appear to have a significant impact on body size indicators,rumen fermentation parameters,or the concentrations of serum total protein and glucose in beef cattle.Furthermore,this approach may yield improved breeding profitability,with a recommended substitution ratio of 2.00%based on the conditions of this experiment. 展开更多
关键词 Potato residue Corn pellet Xinjiang brown cattle Growth performance Rumen fermentation Economic benefit
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Effects of dietary supplementation of probiotic,Clostridium butyricum,on growth performance,immune response,intestinal barrier function,and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88 认领 引用 被引量:59
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作者 Ling Zhang Lingling Zhang +5 位作者 Xiu'an Zhan Xinfu Zeng Lin Zhou Guangtian Cao An'guo Chen Caimei Yang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2016年第1期107-115,共9页
Background: Colibacillosis caused by enterotoxigenic Escherichia coil (E. coil} results in economic losses in the poultry industry. Antibiotics are usually used to control colibacillosis, however, E. coli has varyin... Background: Colibacillosis caused by enterotoxigenic Escherichia coil (E. coil} results in economic losses in the poultry industry. Antibiotics are usually used to control colibacillosis, however, E. coli has varying degrees of resistance to different antibiotics. Therefore the use of probiotics is becoming accepted as an alternative to antibiotics. In this study, we evaluated the effects of Clostfidium butyricum (C. butyficum) on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Eschefichia coli (E. coil) K88. Methods: The chickens were randomly divided into four treatment groups for 28 days. Negative control treatment (NC) consisted of birds fed a basal diet without E. coil K88 challenge and positive control treatment (PC) consisted of birds fed a basal diet and challenged with E. coil K88. C. buOicum probiotic treatment (CB) consisted of birds fed a diet containing 2 x 107 cfu C. buOicum/kg of diet and challenged with E. coil K88. Colistin sulfate antibiotic treatment (CS) consisted of birds fed a diet containing 20 mg colistin sulfate/kg of diet and challenged with E. coil K88. Results: The body weight (BW) and average day gain (ADG) in the broilers of CB group were higher (P 〈 0.05) than the broilers in the PC group overall except the ADG in the 14-21 d post-challenge. The birds in CB treatment had higher (P 〈 0.05) concentration of tumor necrosis factor-a (TNF-a) at 3 and 7 d post-challenge, and higher (P 〈 0.05) concentration of interleukin-4 (IL-4) at 14 d post-challenge than those in the PC treatment group. The concentration of serum endotoxin in CB birds was lower (P 〈 0.05) at 21 d post-challenge, and the concentrations of serum diamine oxidase in CB birds were lower (P 〈 0.05) at 14 and 21 d post-challenge than in PC birds. Birds in CB treatment group had higher (P 〈 0.05) jejunum villi height than those in PC, NC, or CS treatment at 7, 14, and 21 d post-challenge. In comparison to PC birds, the CB birds had lower (P 〈 0.05) jejunum crypt depth during the whole experiment. The birds in CB or CS treatment group had higher (P 〈 0.05) activities of amylase and protease at 3, 7, and 14 d post-challenge, and higher (P 〈 0.05) activity of lipase at 3, 7 d post-challenge than PC birds. 展开更多
关键词 Broiler chickens Clostridium butyricum Digestive enzyme activity Escherichia coli K88 Growth performance Immune response Intestinal barrier
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Effects of dietary protein/energy ratio on growth performance,carcass trait,meat quality,and plasma metabolites in pigs of different genotypes 认领 引用 被引量:54
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作者 Yingying Liu Xiangfeng Kong +6 位作者 Guoli Jiang Bi'e Tan Jinping Deng Xiaojian Yang Fengna Li Xia Xiong Yulong Yin 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2015年第4期435-444,共10页
Background: The protein/energy ratio is important for the production performance and utilization of available feed resources by animals. Increased protein consumption by mammals leads to elevated feed costs and incre... Background: The protein/energy ratio is important for the production performance and utilization of available feed resources by animals. Increased protein consumption by mammals leads to elevated feed costs and increased nitrogen release into the environment. This study aimed to evaluate the effects of dietary protein/energy ratio on the growth performance, carcass traits, meat quality, and plasma metabolites of pigs of different genotypes. Methods: Bama mini-pigs and Landrace pigs were randomly assigned to two dietary treatment groups (Chinese conventional diet with low protein/energy ratio or National Research Council diet with high protein/energy ratio; n = 24 per treatment) in a 2 x 2 factorial arrangement. Blood and muscle samples were collected at the end of the nursery, growing, and finishing phases. Results: We observed significant interactions (P 〈 0.05) between breed and diet for total fat percentage, intramuscular fat (IMF) content, protein content in biceps femoris (BF) muscle, and plasma urea nitrogen (UN) concentration in the nursery phase; for average daily gain (ADG), average daily feed intake (ADFI), dry matter, IMF content in psoas major (PM) muscle, and plasma total protein and albumin concentrations in the growing phase; and for drip loss and plasma UN concentration in the finishing phase. Breed influenced (P 〈 0.05) growth performance, carcass traits, and meat quality, but not plasma metabolites. Throughout the trial, Landrace pigs showed significantly higher (P 〈 0.0_5) ADG, ADFI, dressing percentage, lean mass rate, and loin-eye area than did Bama mini-pigs, but significantly lower (P 〈 0.0.5) feed/gain ratio, fat percentage, backfat thickness, and IMF content. Dietary protein/energy ratio influenced the pH value, chemical composition of BF and PM muscles, and plasma activities of glutamic-pyruvic transaminase and gamma-glutamyl transpeptidase, and plasma concentration of UN. Conclusions: Compared with Landrace pigs, Bama mini-pigs showed slower growth and lower carcass performance, but had better meat quality. Moreover, unlike Landrace pigs, the dietary protein/energy ratio did not affect the growth performance of Bama mini-pigs. These results suggest that, in swine production, low dietary protein/energy ratio may be useful for reducing feed costs and minimizing the adverse effects of ammonia release into the environment. 展开更多
关键词 Dietary protein/energy ratio Growth performance Meat quality Mini-pig Plasma metabolites
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Effects of different forms of yeast Saccharomyces cerevisiae on growth performance,intestinal development,and systemic immunity in early-weaned piglets 认领 引用 被引量:32
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作者 Zongyong Jiang Shaoyong Wei +8 位作者 Zhilin Wang Cui Zhu Shenglan Hu Chuntian Zheng Zhuang Chen Youjun Hu Li Wang Xianyong Ma Xuefen Yang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2016年第1期78-85,共8页
The present study was conducted to determine effects of different forms of yeast (Saccharomyces cerevisiae, strain Y200007) on the growth performance, intestinal development, and systemic immunity in early-weaned pi... The present study was conducted to determine effects of different forms of yeast (Saccharomyces cerevisiae, strain Y200007) on the growth performance, intestinal development, and systemic immunity in early-weaned piglets. A total of 96 piglets (14-d old, initial average body weight of 4.5 kg) were assigned to 4 dietary treatments: (1) basa diet without yeast (Control); (2) basal diet supplemented with 3.00 g/kg live yeast (LY); (3) basal diet supplemented with 2.66 g/kg heat-killed whole yeast (HKY); and (4) basal diet supplemented with 3.00 g/kg superfine yeast powders (SFY). Diets and water were provided ad libitum to the piglets during 3-week experiment. Growth performance of piglets was measured weekly. Samples of blood and small intestine were collected at days 7 and 21 of experiment. Dietary supplementation with LY and SFY improved G:F of piglets at days ]-21 of the experiment (P 〈 0.05) compared to Control group. Serum concentrations of growth hormone (GH), triiodothyronine (T3), tetraiodothyronine (T4), and insulin growth factor 1 (iGF-1) in piglets at day 21 of the experiment were higher when fed diets supplemented with LY and SFY than those in Control group (P 〈 0.05). Compared to Control group, contents of serum urea nitrogen of piglets were reduced by the 3 yeast-supplemented diets (P 〈 0.05). Diets supplemented with LY increased villus height and villus-to-crypt ratio in duodenum and jejunum of piglets (P 〈 0.05) compared to other two groups at day 7 of the experiment. Feeding diets supplemented with LY and SFY increased (P 〈 0.05) serum concentrations of IgA, IL-2, and IL-6 levels in piglets compared to Control. The CD4+/CD8+ ratio and proliferation of T-lymphocytes in piglets fed diets supplemented with LY were increased compared to that of Control group at day 7 of the experiment (P 〈 0.05). In conclusion, dietary supplementation with both LY and SFY enhanced feed conversion, small intestinal development, and systemic immunity in early-weaned piglets, with better improvement in feed conversion by dietary supplementation with LY, while dietary supplementation with SFY was more effective in increasing systemic immune functions in early-weaned piglets. 展开更多
关键词 Growth performance Immunity Intestinal development Piglets Saccharomyces cerevisiae Yeast
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Effects of creep feeding and supplemental glutamine or glutamine plus glutamate (Aminogut) on pre- and post-weaning growth performance and intestinal health of piglets 认领 引用 被引量:25
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作者 Rafael A Cabrera James L Usry +4 位作者 Consuelo Arrellano Eduardo T Nogueira Marianne Kutschenko Adam J Moeser Jack Odle 《Journal of Animal Science and Biotechnology》 SCIE CAS 2013年第3期211-222,共12页
Background: Creep feeding is used to stimulate piglet post-weaning feed consumption.L-Glutamine(GLN) is an important source of fuel for intestinal epithelial cells.The objective of this study was to determine the i... Background: Creep feeding is used to stimulate piglet post-weaning feed consumption.L-Glutamine(GLN) is an important source of fuel for intestinal epithelial cells.The objective of this study was to determine the impact of creep feeding and adding GLN or AminoGut(AG;containing glutamine + glutamate) to pre-and post-weaning diets on pig performance and intestinal health.Litters(N = 120) were allotted to four treatments during 14–21 d of lactation: 1) No creep feed(NC,n = 45);2) creep fed control diet(CFCD,n = 45);3) creep fed 1% GLN(CFGLN,n = 15);4) creep fed.88% AG(CFAG,n = 15).After weaning,the NC and CFCD groups were sub-divided into three groups(n = 15 each),receiving either a control nursery diet(NC-CD,CFCD-CD) or a diet supplemented with either GLN(NC-GLN,CFCD-GLN) or with AG(NC-AG,CFCD-AG).Litters that were creep fed with diets containing GLN or AG also were supplemented with those amino acids in the nursery diets(CFGLN-GLN,CFAG-AG).Glutamine was added at 1% in all three post-weaning diet phases and AG was added at.88% in phase 1 and 2 and at.66% in phase 3.Results: Feed conversion(feed/gain) showed means among treatment means close to significance(P = 0.056) and Tukey's test for pairwise mean comparisons showed that Pigs in the CFGLN-GLN group had the best feed conversion(feed/gain) in the first three-week period post-weaning,exceeding(P = 0.044) controls(CFCD-CD) by 34%.The NC-AG group had(P = 0.02) the greatest feed intake in the last three week of the study,exceeding controls(CFCD-CD) by 12%.CFGLN-GLN,CFCD-GLN and sow reared(SR) pigs had the greatest(P = 0.049) villi height exceeding the CFCD-AG group by 18%,20% and 19% respectively.The CFAG-AG group had the deepest(P = 0.001) crypts among all treatments.CFGLN-GLN,CFCD-GLN and SR groups had the greatest(P = 0.001) number of cells proliferating(PCNA) exceeding those in the NC-CD group by 43%,54% and 63% respectively.Sow reared pigs showed the greatest(P = 0.001) intestinal absorption capacity for xylose and mannitol.Conclusion: Supplementation of creep feed and nursery diets with GLN and/or AminoGut in the first three week improved feed conversion possibly due to improved intestinal health. 展开更多
关键词 Creep feeding Glutamine Growth performance Intestinal health SEM Villi
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Effect of Glycyrrhiza uralensis Fisch polysaccharide on growth performance and immunologic function in mice in Ural City,Xinjiang 认领 引用 被引量:30
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作者 Jie Chen Xiao-Qing Zhu +5 位作者 Li Yang Yan Luo Meng-Yuan Wang Xiao-Ting Liu Ke-Xun Liang Xin-Li Gu 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2016年第11期1056-1061,共6页
Objective:To discuss the effect of Glycyrrhiza uralensis(G.uralensis) Fisch polysaccharide on growth performance and immunologic function in mice in Ural City,Xinjiang and to provide important data supporting the appl... Objective:To discuss the effect of Glycyrrhiza uralensis(G.uralensis) Fisch polysaccharide on growth performance and immunologic function in mice in Ural City,Xinjiang and to provide important data supporting the application of Glycyrrhiza polysaccharide.Methods:A total of100 Kunming mice aged 3 weeks old were randomly divided into 5 groups with 20 mice in each group(10 were females and 10 were males).About 0.5 mL normal saline was given to the mice of control group every day and 0.5 mL G.uralensis Fisch polysaccharide was given to the mice of other groups at the concentration of 1,20,50 and 100 mg/mL respectively.The growth performance(average body weight,average daily feed intake and feed efficiency),immune organ indexes(spleen index and thymus index) and immunologic function(serum IL-2,CD4^+/CD8^+ and the activity of NK cells) of mice in each group were detected continuously.Results:The average body weight,feed efficiency,serum IL-2,CD4^+/CD8^+ and the activity of NK cells of mice were increased with the increase of administrated time after administrating G.uralensis Fisch polysaccharide and were reached up the largest level on Day 28.At the same time,each index was proportional to the given dose and was significantly higher than those of control group and reached up the largest level at the administrated dose of 100 mg/mL.After administrating G.uralensis Fisch polysaccharide,the spleen index and thymus index of mice were increased with the increase of administrated dose and the spleen index and thymus index of mice administrated with the dose of 100 mg/mL were maximum which was more than 1.51 times and 1.43 times of that in control group respectively and the comparative differences showed statistical significance(P0.05).Conclusions:G.uralensis Fisch polysaccharide can significantly improve the growth performance and immunologic function of mice and laid a research basis for the clinical application of G.uralensis Fisch polysaccharide. 展开更多
关键词 Glycyrrhiza uralensis Fisch polysaccharide Immune organ index Growth performance Immunologic function
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Intrauterine growth restriction alters growth performance, plasma hormones,and small intestinal microbial communities in growing-finishing pigs 认领 引用 被引量:13
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作者 Liang Xiong Jinming You +4 位作者 Wanghong Zhang Qian Zhu Francois Blachier Yulong Yin Xiangfeng Kong 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第1期340-357,共18页
Background: The interaction of the gut microbiota with key metabolic and physiological processes may be associated with poor growth outcomes in animals born with intrauterine growth restriction(IUGR).Results: Growth p... Background: The interaction of the gut microbiota with key metabolic and physiological processes may be associated with poor growth outcomes in animals born with intrauterine growth restriction(IUGR).Results: Growth performance, plasma hormone concentrations, and intestinal microbiota composition were analyzed in IUGR pigs and in normal birth weight(NBW) pigs when the NBW pigs reached 25, 50, and 100 kg of body weight(BW). Compared to NBW pigs, IUGR pigs had lower initial, weaned, and final BW, and lower average daily gain and average daily feed intake in all the considered time points. In the 25 kg BW group, IUGR pigs had higher concentrations of plasma ghrelin and pancreatic polypeptide(PP), but lower insulin concentration than NBW pigs, while the situation was reversed in the 50 kg BW group. As compared to NBW pigs, IUGR pigs had higher microbial alpha diversity in the jejunum and ileum;in the 50 and 100 kg BW groups, IUGR pigs had higher Firmicutes abundance but lower Proteobacteria abundance in the jejunum, and lower Lactobacillus abundance in the jejunum and ileum;in the 25 kg BW group, IUGR pigs showed higher unclassified Ruminococcaceae abundance in the ileum;and in 25 and 50 kg BW groups, IUGR pigs showed lower Ochrobactrum abundance in the jejunum.Spearman's correlation revealed that Lactobacillus was negatively correlated with growth performance, while unclassified Ruminococcaceae was positively correlated. Predictive metagenomic analysis detected significantly different expression of genes in the intestinal microbiota between IUGR and NBW pigs, suggesting different metabolic capabilities between the two groups.Conclusions: Growing-finishing IUGR pigs showed lower growth performance, higher microbial alpha diversity, and differences in plasma hormone concentrations compared to NBW pigs. Alterations in the abundance of Firmicutes,Proteobacteria, Ruminococcaceae, Lactobacillus, and Ochrobactrum in the small intestine may be associated with IUGR, and may therefore serve as a future target for gut microbiota intervention in growing-finishing IUGR pigs. 展开更多
关键词 Growing-finishing pigs Growth performance Intrauterine growth restriction Microbial community Small intestine
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Changes in the gut microbiota mediate the differential regulatory effects of two glucose oxidases produced by Aspergillus niger and Penicillium amagasakiense on the meat quality and growth performance of broilers 认领 引用 被引量:10
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作者 Shengru Wu Xiaodong Chen +3 位作者 Taohuan Li Hao Ren Lixin Zheng Xiaojun Yang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2020年第4期1232-1244,共13页
Background: Glucose oxidase(GOD), an aerobic dehydrogenase, has been used as an antibiotic substitute in feed.A study was conducted to evaluate the differential effects of 2 different GODs fermented by Aspergillus nig... Background: Glucose oxidase(GOD), an aerobic dehydrogenase, has been used as an antibiotic substitute in feed.A study was conducted to evaluate the differential effects of 2 different GODs fermented by Aspergillus niger or Penicillium amagasakiense on caecal microbiota and to further illuminate the potential roles of changes in the gut microbiota in regulating the growth performance and meat quality of broiler chickens.Results: A total of 420 one-day-old healthy Arbor Acres broilers were randomly assigned to 4 treatments: the control group,the antibiotic growth promoter(AGP) supplementation group, and the GOD-A and GOD-P(GODs produced by A. niger and P. amagasakiense, respectively) groups. As a result, supplementation with GOD produced by P. amagasakiense could significantly improve the average daily weight gain and average daily feed intake of broilers before 21 days of age by significantly increasing the enzymatic activities of jejunal amylase and those of ileal amylase, chymotrypsin, and lipase in21-day-old broilers and could increase the enzymatic activities of duodenal amylase, jejunal amylase and lipase, and ileal chymotrypsin and lipase in 42-day-old broilers. Meanwhile, compared with AGP treatment, supplementation with GOD produced by P. amagasakiense significantly decreased the L value of 21-day-old broilers and the Δp H and L* value of 42-day-old broilers, while supplementation with GOD produced by A. niger significantly increased the p H24 hvalue of 21-day-old and 42-day-old broilers by reducing plasma malondialdehyde content. By using 16 S r RNA sequencing, we found that the beneficial bacteria and microbiota in broilers were not disturbed but were improved by GOD supplementation compared with ADP treatment, including the genera Eubacterium and Christensenel a and the species uncultured_Eubacterium_sp,Clostridium_asparagiforme, and uncultured_Christensenel a_sp, which were positively related to the improved intestinal digestive enzymatic activities, growth performance, and meat quality of broilers.Conclusion: The altered gut microbiota induced by supplementation with glucose oxidase produced by P. amagasakiense mediate better regulatory effects on the meat quality and growth performance of broilers than that induced by supplementation with glucose oxidase produced by A. niger. 展开更多
关键词 Broiler Glucose oxidase Growth performance Gut function Gut microbiota
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Effects of dietary supplementation with Clostridium butyricum on the growth performance and humoral immune response in Miichthys miiuy 认领 引用 被引量:29
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作者 SONG Zeng-fu WU Tian-xing +1 位作者 CAI Li-sheng ZHENG Xiao-dong 《Journal of Zhejiang University-SCIENCE B》 EI CAS 2006年第7期596-602,共7页
The effects of dietary supplementation with Clostridium butyricum on growth performance and humoral immune response in Miichthys miiuy were evaluated. One hundred and fifty Miichthys miiuy weighing approximately 200-2... The effects of dietary supplementation with Clostridium butyricum on growth performance and humoral immune response in Miichthys miiuy were evaluated. One hundred and fifty Miichthys miiuy weighing approximately 200-260 g were divided into five groups and reared in 15 tanks with closed circuiting culture system. The animals were fed 5 diets: basal diet only (control) or supplemented of the basal diet with C. butyricum at doses of 10^3 (CB1), 10^5 (CB2), 10^7 (CB3) or 10^9 (CB4) CFU/g. Compared with the control, the serum phenoloxidase activity was significantly increased by the supplementation (P〈0.05), acid phosphatases activity was increased significantly (P〈0.05) at the doses of 10^9 CFU/g. Serum lysozyme activity peaked at dose of 10^7 CFU/g and in the skin mucus at dose of 10^9 CFU/g. Immunoglobulin M level in the serum and skin mucus was increased except at dose of 10^3 CFU/g (P〈0.05). The growth at the dose of 10^9 CFU/g was higher than that of the control (P〈0.05). It is concluded that supplementation of C. butyricum can mediate the humoral immune responses and improve the growth performance in Miichthys miiuy. 展开更多
关键词 Clostridium butyricum Growth performance Humoral immune response Miichthys miiuy
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Effects of selenium source and level on growth performance, antioxidative ability and meat quality of broilers 认领 引用 被引量:8
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作者 WANG Chuan-long XING Guan-zhong +5 位作者 WANG Li-sai LI Su-fen ZHANG Li-yang LU Lin LUO Xu-gang LIAO Xiu-dong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第1期227-235,共9页
This experiment was conducted to investigate the effects of selenium(Se) source and level on growth performance, carcass traits, antioxidative ability and meat quality of broilers. A total of 320 one-d-old Arbor Acres... This experiment was conducted to investigate the effects of selenium(Se) source and level on growth performance, carcass traits, antioxidative ability and meat quality of broilers. A total of 320 one-d-old Arbor Acres commercial broilers were randomly assigned to 1 of 5 treatments with 8 replicates in a completely randomized design involving a 2×2 factorial arrangement of treatments plus one Se-unsupplemented basal diet control for 42 d. The two Se sources were sodium selenite and Se yeast, and the two supplemental Se levels were 0.20 and 0.40 mg Se kg^–1. The results showed that broilers fed the Se-supplemented diets had higher(P<0.05) average daily gain and average daily feed intake from 22 to 42 d of age, eviscerated yield and abdominal fat percentages, Se concentrations and glutathione peroxidase(GSH-Px) activities in breast and thigh muscles on d 42, and lower(P<0.05) feed/gain from 1 to 21 and 22 to 42 d of age, mortality from 22 to 42 d of age and malondialdehyde(MDA) concentration in thigh muscle on d 42 than those fed the control diet. Broilers fed the diets supplemented with Se yeast had higher(P<0.05) p H value and lower(P<0.05) shear force in thigh muscle than those fed the diets supplemented with sodium selenite. Additionally, broilers fed the diets supplemented with 0.40 mg Se kg^–1 had lower(P<0.05) shear force in thigh muscle and higher(P<0.05) GSH-Px activities in breast and thigh muscles than those fed the diets supplemented with 0.20 mg Se kg^–1. Furthermore, broilers fed the diet supplemented with Se yeast at 0.40 mg Se kg^–1 had higher(P<0.05) Se concentrations in breast and thigh muscles than those fed the diet supplemented with Se yeast at 0.20 mg Se kg^–1, but no differences(P<0.05) were observed in these indices of broilers fed the diets supplemented with sodium selenite between 0.20 and 0.40 mg Se kg^–1. The results from the present study indicated that supplemental Se could increase the growth performance, muscle Se concentration and antioxidative ability of broilers;and the Se from Se yeast was more effective than the Se from sodium selenite in improving meat quality of broilers. 展开更多
关键词 selenium source growth performance meat quality antioxidative ability broiler
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Supplementation of different fat sources affects growth performance and carcass composition of finishing pigs 认领 引用 被引量:7
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作者 Yanhong Liu Dong Yong Kil +2 位作者 Victor G.Perez-Mendoza Minho Song James E.Pettigrew 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2018年第4期949-956,共8页
Background: There are various fat sources with different energy values and fatty acid compositions that may affect growth performance and carcass composition of grow-finishing pigs. A higher net energy was recently re... Background: There are various fat sources with different energy values and fatty acid compositions that may affect growth performance and carcass composition of grow-finishing pigs. A higher net energy was recently reported in choice white grease compared with soybean oil. Therefore, two experiments were conducted to determine whether practical responses confirm that difference between choice white grease and soybean oil, and to extend the observations to other fat sources.Results: In Exp. 1, pigs fed fats had lower(P < 0.05) average daily feed intake in phase II and overall period,greater(P < 0.05) gain:feed in phase I, phase II, and overall period than pigs fed the control diet. Pigs fed fats tended(P = 0.057) to have thicker backfat depth at the last rib than those fed control. Pigs fed 6% fats had greater(P < 0.01) gain:feed in phase II and overall period than pigs fed 3% fats. During phase I, pigs fed choice white grease grew faster(P < 0.05) than pigs fed soybean oil. In Exp. 2, pigs fed dietary fats(soybean oil, choice white grease, animal-vegetable blend, palm oil, or tallow) had greater(P < 0.01) gain:feed in each phase and overall period, greater(P < 0.01) average daily gain in phase I, but lower(P < 0.01) average daily feed intake in phase II an overall than pigs fed the control diets. The choice white grease also increased(P < 0.05) average daily gain during phase I compared with soybean oil. Pigs fed palm oil had thicker(P < 0.05) backfat depth at the 10 thrib than those fed soybean oil, animal-vegetable blend, or tallow.Conclusions: Inclusion of 6% dietary fat improved feed efficiency of finishing pigs, while different fats produced different practical results that may be consistent with their different energy values. Results from the early stage indicate that dietary fats with relatively more saturated fatty acids may provide greater energy than those with relatively more unsaturated fatty acids for growing pigs. 展开更多
关键词 Carcass composition Dietary fats Energy values Finishing pigs Growth performance
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Effects of Dietary Corn Gluten Meal on Growth Performance and Protein Metabolism in Relation to IGF-I and TOR Gene Expression of Juvenile Cobia (Rachycentron canadum) 认领 引用 被引量:11
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作者 LUO Yiwen AI Qinghui +4 位作者 MAI Kangsen ZHANG Wenbing XU Wei ZHANG Yanjiao LIUFU Zhiguo 《Journal of Ocean University of China》 SCIE CAS 2013年第3期418-426,共9页
A growth experiment was conducted on cobia(Rachycentron canadum,initial weight 108.2 g ± 3.0 g) to investigate the effects of dietary corn gluten meal(CGM) levels on the fish growth,whole body composition and pro... A growth experiment was conducted on cobia(Rachycentron canadum,initial weight 108.2 g ± 3.0 g) to investigate the effects of dietary corn gluten meal(CGM) levels on the fish growth,whole body composition and protein metabolism in relation to specific gene expression.Five isonitrogenous(crude protein 45%) and isoenergetic(gross energy 20 kJ g 1) practical diets were formulated by replacing 0%(the control),17.5%,35.0%,52.5%,and 70.0% of fish meal(FM) protein with CGM protein.No significant differences were observed in the survival,feed intake(FI),specific growth rate(SGR),feed efficiency(FE) and protein productive value(PPV) among fish fed diets with 0%,17.5%,35.0%,and 52.5% of CGM protein.However,these indices were significantly lower in fish fed the diet with 70.0% of CGM protein than those in fish fed the control diet(P < 0.05).The whole-body crude protein and lipid contents were significantly lower while the whole-body moisture content was significantly higher in fish fed the diet with 70.0% of CGM protein compared with the control group(P < 0.05).When 70.0% of FM protein was replaced by CGM,plasma total protein and cholesterol contents were significantly lower than those in the control group(P < 0.05).Fish fed the diet with 70.0% of CGM protein had significantly lower hepatic insulin-like growth factor I(IGF-I) expression levels than those in the control group(P < 0.05).However,no significant differences were observed in hepatic target of rapamycin(TOR),dorsal muscle IGF-I and TOR expression levels among dietary treatments.Results of the present study indicated that 52.5% of FM protein could be replaced by CGM in the diets without significant influences on the growth,feed utilization and protein metabolism of juvenile cobia.The present results might be useful for developing cost effective and sustainable cobia dietary formulations. 展开更多
关键词 Cobia corn gluten meal growth performance protein metabolism gene expression
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Effect of Dietary Supplementation with Hydrolyzed Wheat Gluten on Growth Performance, Cell Immunity and Serum Biochemical Indices of Weaned Piglets (Sus scrofa) 认领 引用 被引量:11
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作者 WANG Xiu-qi FENG You +3 位作者 SHU Gang JIANG Qing-yan YANG Jing-pei ZHANG Zi-feng 《Agricultural Sciences in China》 CSCD 2011年第6期938-945,共8页
To investigate the effects of dietary supplementation with hydrolyzed wheat gluten (HWG) on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed (Large White×andrace)... To investigate the effects of dietary supplementation with hydrolyzed wheat gluten (HWG) on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed (Large White×andrace) and weaned piglets were randomly divided into 4 treatments with 4 replicates of 10 piglets each. The piglets in each treatment were fed an experimental diet containing either 0 g kg-1 HWG (control group), 30 g kg-1 HWG (3% HWG group), 50 g kg-1 HWG (5% HWG group), or 2.5 g kg-1 glycyl-L-glutamine (0.25% Gly-Gln group). The results showed that the diarrhea rates in 3% HWG and 5% HWG groups were significantly lower than in control group from d 1 to 14 (P〈0.05), while the average daily gain (ADG) in each of two groups was increased (P〉0.05). When compared with control group, dietary supplementation with 3% HWG increased the ratio of CD4+:CD8+ and the ratio of serum albumin and globulin concentrations (A:G) on d 14 and 28, as well as the proliferation of T- and B-lymphocytes (P〉0.05) on d 28. In addition, on d 14 and 28, the A:G ratio in 5% HWG group was significantly higher than in control group (P〈0.05), while the ratio of CD4+:CD8+ increased slightly (P〉0.05). Interestingly, 0.25% Gly-Gln group had higher proportion of CD3+ (P〉0.05) and CD4+ (P〈0.05) on d 14 than control group, but growth performances of 0.25% Gly-Gln group were negatively affected at all experiment stages. These results suggested that HWG might improve the growth performance of piglets by strengthening cell immunity and decreasing the occurrence of diarrhea during the prophase after weaning. 展开更多
关键词 hydrolyzed wheat gluten weaned piglets growth performance cell immunity serum biochemical indices
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The Effect of Dietary Supplementation with Phytase Transgenic Corn on Growth Performance,Phosphorus Utilization and Excretion in Growing Pigs (Sus scrofa) 认领 引用 被引量:12
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作者 WANG Xiu-qi WANG Sheng +1 位作者 ZHANG Jun-min YANG Jing-pei 《Agricultural Sciences in China》 CSCD 2011年第5期769-776,共8页
Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn (PTC) on growth performance,phosphorus (P) utilization and excretion in growing pigs.In Exp.1,180 pi... Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn (PTC) on growth performance,phosphorus (P) utilization and excretion in growing pigs.In Exp.1,180 pigs (Large White × Landrace,BW=37.7 kg) were randomly allotted to 4 treatments with 5 replicates of 9 pigs each in order to evaluate the effect of PTC supplementation in low-P diets on growth performance.Four corn soybean meal-based diets consisted of a positive control (PC) diet,a diet containing 500 units (U) of exogenous phytase kg-1 (EP) on the basis of low-P (inorganic P reduced by 0.05% from PC diet) and the low-P+500 (PTC1) or 750 (PTC2) phytase U of PTC kg-1.In Exp.2,20 barrows (Large White×Landrace,BW=31 kg,4 treatments with 5 replicates of 1 pig each) were randomly selected to evaluate the effect of PTC in low-P diets on serum parameters and nutrient utilization.Diets in Exp.2 were similar to those in Exp.1 except that the EP group was replaced by a low-P diet without exogenous phytase supplementation as a negative control (NC) group.The results from Exp.1 showed that the average daily gain (ADG) in the PTC2 group was significantly higher (P〈0.05) than that in the EP group over all periods.On the other hand,the feed:gain (F:G) ratio of the EP group was significantly higher (P〈0.05) than that of the PTC2 group during 1-21 and 1-42 d,respectively.There were no differences in average daily feed intake (ADFI) among all treatments (P〉0.05).The results from Exp.2 showed that the concentration of serum Ca in the NC group was the highest (P〈0.05),while the concentration of serum P in the PTC2 group was the highest (P〈0.05) among all treatments.There was a significant decrease (P〈0.05) in the P apparent digestibility of the NC group compared with the other groups,and that of PTC2 group was the best.Furthermore,fecal P excretion was reduced (P〈0.05) from 1.80 g d-1 in the PC group to 1.28 g d-1 in the PTC2 group.In conclusion,dietary supplementation with PTC could reduce the application of inorganic P,decrease fecal P excretion,and improve the growth performance of growing pigs. 展开更多
关键词 phytase transgenic corn growth performance nutrient apparent digestibility phosphorus excretion growing pigs
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Effects of different proportions of two Bacillus sp. on the growth performance, small intestinal morphology, caecal microbiota and plasma biochemical profile of Chinese Huainan Partridge Shank chickens 认领 引用 被引量:4
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作者 YANG Jia-jun QIAN Kun +3 位作者 WU Dong ZHANG Wei WU Yi-jing XU Ya-yuan 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第6期1383-1392,共10页
A total of 900 one-d-old Chinese Huainan Partridge Shank chickens were randomly allocated into nine groups with five replicates of 20 each.Birds were fed with basal diet,basal diet supplemented with 150 mg kg^(–1) ... A total of 900 one-d-old Chinese Huainan Partridge Shank chickens were randomly allocated into nine groups with five replicates of 20 each.Birds were fed with basal diet,basal diet supplemented with 150 mg kg^(–1) aureomycin,basal diet supplemented with different proportions of Bacillus licheniformis and Bacillus subtilis,which was 0:1.0×10~6,2.5×10~5:7.5×10~5,3.3×10~5:6.6×10~5,5.0×10~5:5.0×10~5,6.6×10~5:3.3×10~5,7.5×10~5:2.5×10~5 and 1.0×10~6:0,respectively.The duration of the experiment was 56 d.The results indicated that dietary supplementation of 6.6×10~5:3.3×10~5 of B.lichenifornis:B.subtilis improved final body weight,increased the average daily gain,and reduced feed/gain ratio(P〈0.05).The numbers of total Lactobacillus and Bifidobacterium sp.in the caecum significantly increased,and the numbers of Escherichia coli and Salmonella sp.significantly declined compared to that of the control(P〈0.05).Intestinal villous height and villous height to crypt depth ratio of the duodenum,jejunum,and ileum were significantly higher than that of the control,and intestinal crypt depth of the duodenum and ileum was significantly lower(P〈0.05).The total antioxidant capacity,total superoxide dismutase,and glutathione peroxidase ability in plasma significantly improved,while the malondialdehyde concentration in plasma decreased(P〈0.05).Compared to the control,plasma concentrations of ammonia,uric acid and urea nitrogen and the activity of xanthine oxidase were reduced(P〈0.05).In conclusion,an inclusion of 6.6×10~5:3.3×10~5 of B.licheniformis:B.subtilis to the diet improved the growth performance,caecal microbiota,plasma biochemical profile,and significantly improved the small intestine morphology,while reducing the mortality rate. 展开更多
关键词 two-strain Bacillus proportion growth performance small intestinal morphology biochemical profile
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