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Seropositivity rates of water channel protein 4 antibodies compared between a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay in neuromyelitis optica patients 认领 引用 被引量：2
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作者 Xiaoli Wu Zhangyuan Liao +3 位作者 Jing Ye Huiqing Dong ChaodongWang Piu Chan 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第32期2490-2494,共5页
A total of 66 samples （from 27 cases with neuromyelitis optica, 26 cases with multiple sclerosis, aa 13 cases with optic neuritis） were tested for aquaporin-4 antibody by a cell-based immunofluorescence assay and an... A total of 66 samples （from 27 cases with neuromyelitis optica, 26 cases with multiple sclerosis, aa 13 cases with optic neuritis） were tested for aquaporin-4 antibody by a cell-based immunofluorescence assay and an enzyme-linked immunosorbent assay. The sensitivities and specificities of the two assays were similar. We further analyzed an additional 68 patients and 93 healthy controls using the enzyme-linked immunosorbent assay. A Kappa test showed good consistency between the two methods in terms of detection of anti-aquaporin-4 antibody in the se of neuromyelitis optica patients. No significant correlations were identified with onset age or disea duration, suggesting that aquaporin-4 antibody is a good marker for neuromyelitis optica. The enzyme-linked immunosorbent assay can be used for quantifying aquaporin-4 antibody concentrations and may be useful to dynamically monitor changes in the levels of aquaporin-4 antibody during disease duration. 展开更多
关键词 neuromyelitis optica cell-based immunofluorescence assay anti-aquaporin 4 antibody enzyme-linked immunosorbent assay long and extended spinal cord lesions neural regeneration
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Applications of multiplexed immunohistochemistry/immunofluorescence and multispectral imaging technology in the field of tumor immunotherapy 认领 引用 被引量：4
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作者 Wenzhe Li Xia Yuan +1 位作者 Bo Xu Shuxiang Song 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2020年第10期734-747,共14页
Multiplexed immunohistochemistry/fluorescence(mIHC/IF)in combination with multispectral unmixing is a novel multitarget histopathological staining and imaging technique.By simultaneously revealing expression level and... Multiplexed immunohistochemistry/fluorescence(mIHC/IF)in combination with multispectral unmixing is a novel multitarget histopathological staining and imaging technique.By simultaneously revealing expression level and spatial information for up to eight biomarkers in situ,in addition to a nuclear stain within a single formalin-fixed paraffin-embedded(FFPE)tissue section,this technology can analyze the phenotype,abundance,morphology and intercellular relationship of cells while providing statistically significant results.In recent years,technical improvements have brought new insight into mIHC/IF and multispectral imaging approaches,which have been successfully applied in the field of cancer immunotherapy,specifically in regard to tumor microenvironment research,immunotherapy drug discovery,and prognostic and metastatic risk evaluation.This review highlights the principle,workflow,advantages and disadvantages of the technology,and discusses the latest applications of mIHC/IF-based imaging technology in the field of TME-related research and immunotherapy drug discovery. 展开更多
关键词 Multiplexed immunohistochemistry/immunofluorescence(mIHC/IF) Multispectral imaging Tumor microenvironment Tumor immunotherapy
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Determination of anti-endomysium IgA antibodies in the diagnosis of celiac disease:Comparison of a novel ELISA-based assay with conventional immunofluorescence 认领 引用 被引量：1
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作者 Dennis CW Poland Huib Ceelie +1 位作者 Rob B Dinkelaar Cornelis Beijer 《World Journal of Gastroenterology》 SCIE CAS 2006年第17期2779-2780,共2页
AIM： To evaluate the novel anti-endomysium （anti-EMA） detection based on ELISA. METHODS： Anti-EMA IgA was measured by a novel ELISA in 196 patients with gastrointestinal symptoms and suspected mal-absorption. Data... AIM： To evaluate the novel anti-endomysium （anti-EMA） detection based on ELISA. METHODS： Anti-EMA IgA was measured by a novel ELISA in 196 patients with gastrointestinal symptoms and suspected mal-absorption. Data were compared with those obtained by the conventional IF test. RESULTS： A good concordance of 98% was found between these two assays. In sera of 161 patients （82%） both assays tested negative whereas in sera of 31 patients （16%） both assays tested positive for the presence of anti-EMA antibodies. Discrepancies between EMA- ELISA and EMA-immunofluorescence （IF） were found in only 4 patients （2%）.CONCLUSION： This ELISA can replace IF for the detection of anti-EMA antibodies and provide clinicians with an excellent tool to screen for celiac disease in patients with gastrointestinal complaints. 展开更多
关键词 Celiac disease Endomysium ELISA Immunofluorescence Histology
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Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections 认领 引用 被引量：1
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作者 Tatjana Vilibic-Cavlek Ljubo Barbic +4 位作者 Vladimir Stevanovic Vladimir Savic Anna Mrzljak Maja Bogdanic Irena Tabain 《World Journal of Methodology》 2021年第6期294-301,共8页
BACKGROUND Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results.AIM To analyze the diagnostic value of indirect immunofluorescence assay(IFA)and western blot(WB)in the di... BACKGROUND Serologic cross-reactivity between hantaviruses often complicates the interpretation of the results.AIM To analyze the diagnostic value of indirect immunofluorescence assay(IFA)and western blot(WB)in the diagnosis of hantavirus infections.METHODS One hundred eighty-eight serum samples from Puumala(PUUV)and Dobrava(DOBV)orthohantavirus infected patients were analyzed.Serology was performed using commercial tests(Euroimmun,Lübeck,Germany).RESULTS Using IFA,49.5%of acute-phase samples showed a monotypic response to PUUV,while 50.5% cross-reacted with other hantaviruses.The overall cross-reactivity was higher for immunoglobulin G(IgG)(50.0%)than for immunoglobulin M(IgM)(25.5%).PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan(12.3%/31.5%),Seoul(7.5%/17.8%),DOBV(5.4%/28.1%),and Saaremaa(4.8%/15.7%).Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan(76.2%/95.2%),Saaremaa(80.9%/83.3%),and Seoul(78.6%/85.7%)and moderate with PUUV(28.5%/38.1%).Using a WB,serotyping was successful in most cross-reactive samples(89.5%).CONCLUSION The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections,confirming serotype in most IFA cross-reactive samples. 展开更多
关键词 Hantaviruses Serology Cross-reactivity Indirect immunofluorescence Western blot
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COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA 认领 引用
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作者 Fang Liu Jia-bi Wang +2 位作者 Yue-hua Liu Ya-gang Zuo Xiao-hong Man 《Chinese Medical Sciences Journal》 CAS 2006年第2期125-127,共3页
Objective To compare the effectiveness of immunofluorescence and polymerase chain reaction （PCR） in detecring human papilloma virus （HPV） in condyloma acuminata （CA）. Methods HPVs in CA tissues from 60 patients ... Objective To compare the effectiveness of immunofluorescence and polymerase chain reaction （PCR） in detecring human papilloma virus （HPV） in condyloma acuminata （CA）. Methods HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism （RFLP） . Resuits The positive detective rates of immunofluorescence and PCR were 56. 67 % （34/60） and 96. 67 % （58/ 60）, respectively （ P 〈 0. 01 ）. RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%. Conclusion The sensibility of PCR is superior to that of immunofluorescence. 展开更多
关键词 condyloma acuminata human papilloma virus polymerase chain reaction immunofluorescence
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Immunofluorescence on paraffin embedded renal biopsies:Experience of a tertiary care center with review of literature 认领 引用 被引量：3
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作者 Geetika Singh Lavleen Singh +2 位作者 Ranajoy Ghosh Devajit Nath Amit Kumar Dinda 《World Journal of Nephrology》 2016年第5期461-470,共10页
AIMTo describe the technique of immunofluorescence on paraffin embedded tissue sections and discuss the po-tential pitfalls with an in depth review of literature.METHODSImmunofluorescence is integral to diagnostic ren... AIMTo describe the technique of immunofluorescence on paraffin embedded tissue sections and discuss the po-tential pitfalls with an in depth review of literature.METHODSImmunofluorescence is integral to diagnostic renal pa-thology. Immunofluorescence on paraffin embedded renal biopsies （IF-P） after enzyme treatment has been described in literature, however has not found widespread use in renal pathology laboratories. In our laboratory proteinase K digestion of paraffn embedded renal biopsy material was standardized and applied prospectively in cases where immunofuorescence on fresh frozen tissue was non contributory or not possible. Diagnostic utility was assessed and in a cohort of cases comparison of intensity of staining with routine immunofuorescence was performed.RESULTSOver the 5-year study period, of the 3141 renal biopsies received IF-P was performed on 246 cases （7.7%） and was interpretable with optimal digestion in 214 cases （6.8%）. It was of diagnostic utility in the majority of cases, which predominantly included glomerular disease. Non-diagnostic IF-P was found in membranous nephropathy （2 of 11 cases）, membranoproliferative glomerulonephritis （2 of 32 cases）, lupus nephritis （1 of 25 cases）, post infectious glomerulonephritis （1 of 11 cases） and chronic glomerulonephritis （3 of 8 cases）. Comparing cases with both routine IF and IF-P, 35 of 37 showed either equal intensity or a minor difference in intensity of staining（1＋） for the diagnostic immunoglobulin/complement. Technically assessment of immunofluorescence on the paraffin embedded tissue was found to be easier with clearly observed morphology, however a false positive staining pattern was observed in under-digested tissue. CONCLUSIONAs a “salvage” technique, immunofuorescence on paraffn embedded renal biopsies is of great diagnostic utility, however not without pitfalls. 展开更多
关键词 Immunofluorescence on paraffin section Renal biopsy Salvage technique Enzymatic digestion Proteinase KSnea
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New highly specific anti-BnASY polyclonal antibody prepara-tion and application in immunofluorescence assay 认领 引用
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作者 Keqi Li Rong Yuan +3 位作者 Xiaohong Yan Xinhua Zeng Ruixing Guo Gang Wu 《Oil Crop Science》 2016年第4期58-64,共7页
ASY （asynaptic） is a synaptonemal complex （SC） related protein in plant mei- osis. Some ASYs in plants have been cloned and functional determined. ASY in Brassica napus has not been sequenced and functional certif... ASY （asynaptic） is a synaptonemal complex （SC） related protein in plant mei- osis. Some ASYs in plants have been cloned and functional determined. ASY in Brassica napus has not been sequenced and functional certificated. Here, we first cloned ASY gene in Brassica napus （BnASY） and inserted it into vector pET-32a for expression fusion protein BnASY-6His in Escherichia coli BL21 （DE3）. Purified fusion protein was used to produce polyclonal antibody. Specificity and application of polyclonal antibody was examined by Western blot and immunofluorescence assay. Results showed that BnASY-6His recombi-nant protein and anti-BnASY antibody were successfully obtained. Polyclonal anti-BnASY antibody had high specificity. Results of immunofluorescence showed that ASY signals appeared at leptotene stage, faded away gradually at pachytene stage and almost disap-peared at diplonema stage. In this study, highly specific polyclonal antibody was success-fully acquired and used for immunofluorescence assay in plant cells. It will contribute to functional research of ASY in B. napus.We thank Zhigang Li for providing pET-32a vector and Escherichia coli BL21 pLysS strains. 展开更多
关键词 Brassica napus BnASY polyclonal antibody immunofluorescence
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The Application of Quantum Dots Double-Labeling Immunofluorescence Technology in Detection of PR and CD146 in Paraffin-Embedded Tissue Sections of Endometrioid Adenocarcinoma 认领 引用
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作者 Jianping Zheng Qing Huang +3 位作者 Lei Zhang Yong Chen Jinpeng Jiang Cunjian Yi 《Yangtze Medicine》 2017年第1期1-7,共7页
Objective: The aim was to detect the expression of PR and CD146 in paraf-fin-embedded tissue sections of endometrioid adenocarcinoma by using QDs double-labeling immunofluorescence, and evaluate the applied value of Q... Objective: The aim was to detect the expression of PR and CD146 in paraf-fin-embedded tissue sections of endometrioid adenocarcinoma by using QDs double-labeling immunofluorescence, and evaluate the applied value of QDs double-labeling immunofluorescence in endometrioid adenocarcinoma. Methods: To detect the expression of PR and CD146 on 140 cases of paraffin-embedded tissue sections of endometrioid adenocarcinoma by using QDS double-labeling immunofluorescence. Results: The co-expression of PR and CD146 in the endometrioid adenocarcinoma can be detected by QDs double-labeling immunofluorescence, and there was no correlation between them (P > 0.05). Conclusion: QDs double-labeling immunofluorescence can detect the localization and co-expression of PR and CD146 in the endometrioid adenocarcinoma. 展开更多
关键词 Endometrioid Adenocarcinoma PR CD146 QDs Double-Labeling Immunofluorescence
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(Giga)TIME for the era of AI:virtual multiplex immunofluorescence from routine H&E for large populations 认领 引用
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作者 Shao-Ping Yang Dihua Yu 《Signal Transduction and Targeted Therapy》 SCIE CSCD 2026年第4期1823-1824,共2页
In a recent paper published in Cell,Valanarasu,Xu,Usuyama,Kim et al.introduced GigaTIME,a multimodal artificial intelligence(AI)framework that generates photorealistic virtual multiplex immunofluorescence(mIF)images f... In a recent paper published in Cell,Valanarasu,Xu,Usuyama,Kim et al.introduced GigaTIME,a multimodal artificial intelligence(AI)framework that generates photorealistic virtual multiplex immunofluorescence(mIF)images from routine hematoxylin and eosin(H&E)-stained pathology slides.1 The study proposes that H&Estained images,paired with large-scale multimodal AI training,can serve as a hypothesis-generating route to population-scale modeling of marker-like virtual mIF images that approximate the tumor immune microenvironment(TIME),enabling spatially informed association screening across real-world patient cohorts that would be impractical to profile with experimental multiplex imaging at comparable scale. 展开更多
关键词 tumor immune mi multimodal artificial intelligence ai framework artificial intelligence spatially informed association screening hematoxylin eosin staining mif images gigatime virtual multiplex immunofluorescence
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Quenching autofluorescence in the alimentary canal tissues of Bactericera cockerelli(Hemiptera:Triozidae)for immunofluorescence labeling 认领 引用 被引量：2
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作者 Xiao-Tian Tang Freddy Ibanez Cecilia Tamborindeguy 《Insect Science》 SCIE CAS CSCD 2020年第3期475-486,共12页
Immunofluorescence has been widely used to localize microbes or specific molecules in insect tissues or cells.However,significant autofluorescence is frequently observed in tissues which can interfere with the fluores... Immunofluorescence has been widely used to localize microbes or specific molecules in insect tissues or cells.However,significant autofluorescence is frequently observed in tissues which can interfere with the fluorescent identification of target antigens,leading to inaccurate or even false positive fluorescent labeling.The alimentary canal of the potato psyllid,Bactericera cockerelliŠulc,exhibits intense autofluorescence,hindering the application of immunolocalization for the detection and localization of the economically important pathogen transmitted by this insect,“Candidatus Liberibacter solanacearum”(Lso).In the present study,we tested the use of irradiation,hydrogen peroxide(H2O2)and Sudan black B(SBB)treatments to reduce the autofluorescence in the B.cockerelli alimentary canal tissues.Furthermore,we assessed the compatibility of the above‐mentioned treatments with Lso immunolocalization and actin staining using phalloidin.Our results showed that the autofluorescence in the alimentary canal was reduced by irradiation,H2O2,or SBB treatments.The compatibility assays indicated that irradiation and H2O2 treatment both greatly reduced the fluorescent signal associated with Lso and actin.However,the SBB incubation preserved those target signals,while efficiently eliminating autofluorescence in the psyllid alimentary canal.Therefore,herein we propose a robust method for reducing the autofluorescence in the B.cockerelli alimentary canal with SBB treatment,which may improve the use of immunofluorescence labeling in this organism.This method may also have a wide range of uses by reducing the autofluorescence in other arthropod species. 展开更多
关键词 endosymbionts gut immunofluorescence Liberibacter pathogen psyllid vector
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Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy 认领 引用 被引量：42
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作者 Wei Chang Colin Tan Sanjna Nilesh Nerurkar +6 位作者 Hai Yun Cai Harry Ho Man Ng Duoduo Wu Yu Ting Felicia Wee Jeffrey Chun Tatt Lim Joe Yeong Tony Kiat Hon Lim 《Cancer Communications》 SCIE 2020年第4期135-153,共19页
Conventional immunohistochemistry(IHC)is a widely used diagnostic technique in tissue pathology.However,this technique is associated with a number of limitations,including high inter-observer variability and the capac... Conventional immunohistochemistry(IHC)is a widely used diagnostic technique in tissue pathology.However,this technique is associated with a number of limitations,including high inter-observer variability and the capacity to label only one marker per tissue section.This review details various highly multiplexed techniques that have emerged to circumvent these constraints,allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition,cellular functional and cell-cell interactions.Among these techniques,multiplex Immunohistochemistry/Immunofluorescence(mIHC/IF)has emerged to be particularly promising.mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible,efficient and cost-effective tissue studies.This technique has immediate potential for translational research and clinical practice,particularly in the era of cancer immunotherapy. 展开更多
关键词 immunofluorescence immunohistochemistry immunotherapy multiplex overview
Research on the Association Between Anti-Nuclear Antibodies (ANA) and Autoimmune Diseases 认领 引用
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作者 LI Zhijie 《外文科技期刊数据库（文摘版）医药卫生》 2026年第6期085-089,共5页
Objective To investigate the distribution characteristics of antinuclear antibodies (ANA) within complex autoimmune pathological systems and establish their correlation with specific immune dysregulation disorders. Me... Objective To investigate the distribution characteristics of antinuclear antibodies (ANA) within complex autoimmune pathological systems and establish their correlation with specific immune dysregulation disorders. Methods A total of 80 patients with confirmed or suspected immune disorders were enrolled and classified into six groups based on clinical manifestations. Antibody concentrations and karyotype information were determined using indirect immunofluorescence, while linear immunoblotting was employed to assess specific antigen-antibody interactions. Results Different pathological groups exhibited distinct patterns in antibody concentration levels, fluorescence staining profiles, and specific antigen recognition signatures: high-concentration homogeneous types were strongly associated with systemic lupus erythematosus, whereas centromeric types were predominantly observed in patients with systemic sclerosis. Conclusion ANA serological profiles demonstrate highly specific associations with autoimmune disease subtypes, and comprehensive multi-parameter analysis contributes to optimizing clinical diagnostic and therapeutic workflows. 展开更多
关键词 Antinuclear antibodies Autoimmune diseases Indirect immunofluorescence method Fluorescent karyotype Specific autoantibodies
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Distribution of Fucosylated Xyloglucans among the Walls of Different Cell Types in Monocotyledons Determined by Immunofluorescence Microscopy 认领 引用
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作者 Maree Brennan Philip J.Harris 《Molecular Plant》 SCIE CSCD 2011年第1期144-156,共13页
Xyloglucans in the non-lignified primary cell walls of different species of monocotyledons have diverse struc-tures,with widely varying proportions of oligosaccharide units that contain fucosylated side chains(F side ... Xyloglucans in the non-lignified primary cell walls of different species of monocotyledons have diverse struc-tures,with widely varying proportions of oligosaccharide units that contain fucosylated side chains(F side chains).To determine whether fucosylated xyloglucans occur in all non-lignified walls in a range of monocotyledon species,we used immunofluorescence microscopy with the monoclonal antibody CCRC-M1.The epitope of this antibody,α-L-FUCp-(1→2)-β-D-Galp,occurs in F side chains.In most non-commelinid monocotyledons,the epitope was found in all non-lignified walls.A similar distribution was found in the palm Phoenix canariensis,which is a member of the basal commelinid order Arecales.However,in the other commelinid orders Zingiberales,Commelinales,and Poales,the occurrence of the epitope was restricted,sometimes occurring in only the phloem walls,but often also in walls of other cell types including stomatal guard and subsidiary cells and raphide idioblasts.No epitope was found in the walls of the commelinids Tradescantia virginiana(Commelinaceae,Commelinales)and Zea mays(Poaceae,Poales),but it occurred in the phloem walls of two other Poaceae species,Lolium multiflorum and L.perenne.The distribution of the epitope is discussed in relation to xyloglucan structures in the different taxa.However,the functional significance of the restricted distributions is unknown. 展开更多
关键词 Commelinid monocotyledons fucosylated xyloglucans immunofluorescence microscopy monoclona antibody CCRC-M1 non-commelinid monocotyledons plant cell wall.
IGF2BP3-mediated m6A modification of RASGRF1 promoting joint injury in rheumatoid arthritis 认领 引用
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作者 Qishun Geng Yi Jiao +10 位作者 Wenya Diao Jiahe Xu Zhaoran Wang Xing Wang Zihan Wang Lu Zhao Lei Yang Yilin Wang Tingting Deng Bailiang Wang Cheng Xiao 《Bone Research》 SCIE CAS CSCD 2025年第4期1015-1027,共13页
With the deepening of epigenetic research,studies have shown that N6-methyladenosine(m6A)is closely related to the development of rheumatoid arthritis(RA),but the mechanism is still unclear.In the study,we collecte... With the deepening of epigenetic research,studies have shown that N6-methyladenosine(m6A)is closely related to the development of rheumatoid arthritis(RA),but the mechanism is still unclear.In the study,we collected synovial tissues from normal controls and patients with osteoarthritis(OA)or RA.The levels of m6A and inflammation were analyzed by immunofluorescence staining and western blotting.The roles of IGF2BP3 in cell proliferation and inflammatory activation were explored using transfection and RNA immunoprecipitation assays.IGF2BP3−/−mice were generated and used to establish an arthritis mouse model by transferring serum from adult arthritis K/BxN mice.We found m6A levels were markedly increased in RA patients and mouse models,and the expression of IGF2BP3 was upregulated in individuals with RA and related to the levels of inflammatory markers.IGF2BP3 played an important part in RA-fibroblast-like synoviocytes(FLS)by promoting cell proliferation,migration,invasion,inflammatory cytokine release and inhibiting autophagy.In addition,IGF2BP3 inhibited autophagy to reduce ROS production,thereby decreasing the inflammatory activation of macrophages.More importantly,RASGRF1-mediated mTORC1 activation played a crucial role in the ability of IGF2BP3 to promote cell proliferation and inflammatory activation.In an arthritis model of IGF2BP3−/−mice,IGF2BP3 knockout inhibited RA-FLS proliferation and inflammatory infiltration,and further ameliorated RA joint injury.Our study revealed an important role for IGF2BP3 in RA progression.The targeted inhibition of IGF2BP3 reduced cell proliferation and inflammatory activation and limited RA development,providing a potential strategy for RA therapy. 展开更多
关键词 epigenetic researchstudies transfection rna cell proliferation immunofluorescence staining synovial tissues m inflammatory activation western blottingthe
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Analysis of antinuclear antibody pattern distribution and correlation in patients with colorectal cancer 认领 引用
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作者 Zi-Zhen Liang Jin-Hua He +2 位作者 Ze-Ping Han Xiao-Yan Yang Li-Yin Liao 《World Journal of Gastrointestinal Surgery》 SCIE 2025年第12期81-88,共8页
BACKGROUND Patients with tumors often develop multiple autoantibodies against tumorassociated antigens.Among these,antinuclear antibodies(ANAs)constitute a clinically important group distributed across the nucleus,cyt... BACKGROUND Patients with tumors often develop multiple autoantibodies against tumorassociated antigens.Among these,antinuclear antibodies(ANAs)constitute a clinically important group distributed across the nucleus,cytoplasm,and cytoskeleton.Emerging evidence suggests that ANAs are closely associated with the development and progression of various malignancies,including colorectal cancer(CRC).AIM To detect ANA fluorescence patterns in CRC using indirect immunofluorescence(IIF)and investigate their correlation with the disease.METHODS We collected serum samples from patients and healthy controls visiting The Affiliated Panyu Central Hospital of Guangzhou Medical University between May 2023 and March 2024 for analysis.The study included 38 patients with newly diagnosed CRC,43 patients with colorectal polyps(CRP),and 29 healthy controls.Serum ANA expression was assessed by IIF,and fluorescence patterns were recorded for each group.Differences in ANA titers were compared among each group to analyze the differences in serum ANA-positive expression,which were further analyzed to explore the correlation between ANA expression and CRC screening.RESULTS ANA positivity rates were 50.00%in the CRC group,46.51%in the colorectal polyp group,and 6.90%in the healthy control group,with significantly higher rates in the two patient groups compared to the control group(P<0.05).In the CRC group,the most common fluorescence patterns were nuclear speckled(15.79%)and cytoplasmic speckled(15.79%),with titers predominantly low(1:100,28.95%).In the colorectal polyp group,nuclear speckled(18.60%)and nuclear homogeneous(11.63%)were the most frequent,with titers also predominantly low(1:100,37.21%).The distribution of intermediate titers differed significantly among groups(P<0.05).CONCLUSION ANAs are associated with both CRP and CRC and may be useful in early CRC screening.Medium-to-high ANA titers,in particular,should prompt further evaluation for possible CRC correlation.Multiple ANA fluorescence patterns can be detected across all groups,with patients with CRP and CRC showing greater pattern diversity than healthy controls. 展开更多
关键词 Colorectal cancer Antinuclear antibodies Colorectal polyps Indirect immunofluorescence Distribution of titers
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Revolutionizing Tissue Clearing and 3-Dimensional Imaging:Transparent Embedding Solvent System for Uniform High-Resolution Imaging 认领 引用
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作者 Yating Yi Hu Zhao 《Biomedical Engineering Frontiers》 EI CAS 2025年第1期583-587,共5页
Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional(3D)imaging.A newly published transparent embedding system,TESOS(Transparent Embedding Solvent ... Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional(3D)imaging.A newly published transparent embedding system,TESOS(Transparent Embedding Solvent System),ensures consistent submicron resolution imaging throughout the entire sample,and can be compatible with different microscopy systems.This method shows great potential in connectome mapping,and might be an optimal option for future 3D multiplex immunofluorescence and RNA in situ hybridization imaging.Additional efforts would be needed to innovate labeling,imaging,and data processing strategies to fully utilize the potential of transparent embedding systems in highresolution imaging of large-scale samples. 展开更多
关键词 rna situ hybridizat connectome mappingand microscopy systemsthis tissue clearing submicron resolution imaging d multiplex immunofluorescence embedding solvent system ensures transparent embedding
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Erratum to:Stereotactic Injection of shRNA GSK-3β-AAV Promotes Axonal Regeneration after Spinal Cord Injury 认领 引用
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作者 Yu-chao Zuo Nan-xiang Xiong Hong-yang Zhao 《Current Medical Science》 SCIE CAS CSCD 2025年第1期154-155,共2页
Erratum to:J Huazhong Univ Sci Technol[Med Sci]36(4):548–553,2016 http://gffzzd3cc09b8251d45dfsbubqwpk60oxu6b9q.ffgz.tsg.suse.edu.cn/10.1007/s11596-016-1623-6 In the originally published article(http://gffzzd3cc09b8251d45dfsbubqwpk60oxu6b9q.ffgz.tsg.suse.edu.cn/10.1007/s11596-016-1623-6),the immunofluorescence images... Erratum to:J Huazhong Univ Sci Technol[Med Sci]36(4):548–553,2016 http://gffzzd3cc09b8251d45dfsbubqwpk60oxu6b9q.ffgz.tsg.suse.edu.cn/10.1007/s11596-016-1623-6 In the originally published article(http://gffzzd3cc09b8251d45dfsbubqwpk60oxu6b9q.ffgz.tsg.suse.edu.cn/10.1007/s11596-016-1623-6),the immunofluorescence images in shRNA group in Fig.3 were accidentally used rather than the final,formal experiments.To retain consistency,the entire Fig.3 is replaced here with original images of the experiments.The authors declare that this correction will not affect the conclusion of the study. 展开更多
关键词 spinal cord injury stereotactic injection GSK axonal regeneration immunofluorescence images shrna AAV shrna group
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Prevalence and Risk Factors for Cryptosporidium Diarrhea among Children Aged Five Years and below in Selected Health Institutions in Abakaliki, South-East Nigeria 认领 引用
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作者 Onyinye Ifeyinwa Nkeiru Onyekachi Uduma Victor Uduma +14 位作者 Emeka Donald Ogiji Kenechukwu Emmanuel Onyekachi Nweke Chinedu Idakari Nneka Marian Chika-Igenyi Chidiebere Brown Ene Chinwe Ifeoma Joe-Akunne Chinedum Christabel Amagwu Shedrach Chikezie Emeribe Felix Osogu Edegbe Bolaji Abdulazeez Akanni Chibuike Sunday Ugwuocha Kingsley Achi Adamma Gloria Olisa Uzochukwu Chimdindu Ibe Chiedozie Kingsley Ojide 《Advances in Microbiology》 CAS 2025年第1期1-18,共18页
Background: Diarrheal diseases have globally decreased over the past few decades, yet they remain one of the top three causes of mortality in children under five years, especially in sub-Saharan Africa and Nigeria. Se... Background: Diarrheal diseases have globally decreased over the past few decades, yet they remain one of the top three causes of mortality in children under five years, especially in sub-Saharan Africa and Nigeria. Seasonal peaks of diarrheal episodes continue to contribute significantly to childhood mortality in these regions. One of the notable causes of diarrhea in children is parasitic infections, particularly Cryptosporidium, which poses a serious health risk. In Nigeria, the burden of Cryptosporidium diarrhea is under-researched, making it imperative to investigate its prevalence and associated risk factors. Study Objectives: The study aims to determine the prevalence and risk factors associated with Cryptosporidium diarrhea among children aged five years and below in selected health institutions in Abakaliki, South-East Nigeria. Methodology: This cross-sectional study was conducted from January to May 2017, recruiting 200 children under five years with diarrhea from health institutions in Abakaliki. Fecal specimens were analyzed for Cryptosporidium oocysts using light microscopy with modified Ziehl-Neelsen staining and immunofluorescent antibody test (IFAT). Deoxyribonucleic acid (DNA) was extracted from positive samples using QIAmp® DNA stool kit, followed by Polymerase Chain Reaction (PCR) and molecular genotyping. Results: Cryptosporidium was detected in 0.5% (1/200) of children via light microscopy and 6.5% (13/200) via IFAT. All positive samples were confirmed as Cryptosporidium hominis by PCR. The prevalence of infection was significantly higher in children from institutionalized homes (50.0%) compared to monogamous homes (6.2%) (p Conclusion: Cryptosporidium hominis is a notable cause of diarrhea among children in Abakaliki, primarily transmitted through human-to-human contact. The study underscores the need for targeted interventions in childcare institutions to prevent outbreaks. Health authorities should promote breastfeeding and enhance education on hygiene practices in vulnerable populations. 展开更多
关键词 Cryptosporidium hominis Immunofluorescent Antibody Test Ziehl-Neelsen Stain
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Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry 认领 引用 被引量：5
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作者 Mostafa K El-Awady Ashraf A Tabll +4 位作者 El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy 《World Journal of Gastroenterology》 SCIE CAS 2005年第33期5203-5208,共6页
AIM： We designed two synthetic-core-specific peptides core 1 （C1） and core 2 （C2）, and an E1-specific peptide （El）. We produced specific polyclonal antibodies against these peptides and used the antibodies for ... AIM： We designed two synthetic-core-specific peptides core 1 （C1） and core 2 （C2）, and an E1-specific peptide （El）. We produced specific polyclonal antibodies against these peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes. METHODS： Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for i h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry. RESULTS： After 1 h of incubation, antibodies against C1, C2, and El detected HCV antigens on the surface of 27%, 26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection. Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection. CONCLUSION： Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle. Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. 展开更多
关键词 Flow cytometry Hepatitis C virus Envelope Core Antibodies Indirect immunofluorescence Minus and plus RNA strand Peripheral blood mononudear cells
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Elastic modulus affects the growth and differentiation of neural stem cells 认领 引用 被引量：4
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作者 Xian-feng Jiang Kai Yang +4 位作者 Xiao-qing Yang Ying-fu Liu Yuan-chi Cheng Xu-yi Chen Yue Tu 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第9期1523-1527,共5页
It remains poorly understood if carrier hardness, elastic modulus, and contact area affect neural stem cell growth and differentiation. Tensile tests show that the elastic moduli of Tiansu and SMI silicone membranes a... It remains poorly understood if carrier hardness, elastic modulus, and contact area affect neural stem cell growth and differentiation. Tensile tests show that the elastic moduli of Tiansu and SMI silicone membranes are lower than that of an ordinary dish, while the elastic modulus of SMI silicone membrane is lower than that of Tiansu silicone membrane. Neural stem cells from the cerebral cortex of embryonic day 16 Sprague-Dawley rats were seeded onto ordinary dishes as well as Tiansu silicone membrane and SMI silicone membrane. Light microscopy showed that neural stem cells on all three carriers show improved adherence. After 7 days of differentiation, neuron specific enolase, glial fibrillary acidic protein, and myelin basic protein expression was detected by immunofluorescence. Moreover, flow cytometry revealed a higher rate of neural stem cell differentiation into astrocytes on Tiansu and SMI silicone membranes than on the ordinary dish, which was also higher on the SMI than the Tiansu silicone membrane. These findings con- firm that all three cell carrier types have good biocompatibility, while SMI and Tiansu silicone membranes exhibit good mechanical homogenization. Thus, elastic modulus affects neural stem cell differentiation into various nerve cells. Within a certain range, a smaller elastic modulus re- sults in a more obvious trend of cell differentiation into astrocytes. 展开更多
关键词 nerve regeneration neural stem cells carrier mechanical properties elastic modulus cell differentiation neurons immunofluorescence astrocytes neural regeneration
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