Knowledge-Based Engineering (KBE) is introduced into the ship structural design in this paper. From the implementation of KBE, the design solutions for both Rules Design Method (RDM) and Interpolation Design Meth...Knowledge-Based Engineering (KBE) is introduced into the ship structural design in this paper. From the implementation of KBE, the design solutions for both Rules Design Method (RDM) and Interpolation Design Method (IDM) are generated. The corresponding Finite Element (FE) models are generated. Topological design of the longitudinal structures is studied where the Gaussian Process (GP) is employed to build the surrogate model for FE analysis. Multi-objective optimization methods inspired by Pareto Front are used to reduce the design tank weight and outer surface area simultaneously. Additionally, an enhanced Level Set Method (LSM) which employs implicit algorithm is applied to the topological design of typical bracket plate which is used extensively in ship structures. Two different sets of boundary conditions are considered. The proposed methods show satisfactory efficiency and accuracy.展开更多
Knowledge-based engineering(KBE) has made success in automobile and molding design industry, and it is introduced into the ship structural design in this paper. From the implementation of KBE, the deterministic design...Knowledge-based engineering(KBE) has made success in automobile and molding design industry, and it is introduced into the ship structural design in this paper. From the implementation of KBE, the deterministic design solutions for both rules design method(RDM) and interpolation design method(IDM) are generated. The corresponding finite element model is generated. Gaussian process(GP) is then employed to build the surrogate model for finite element analysis, in order to increase efficiency and maintain accuracy at the same time, and the multi-modal adaptive importance sampling method is adopted to calculate the corresponding structural reliability.An example is given to validate the proposed method. Finally, the reliabilities of the structures' strength caused by uncertainty lying in water corrosion, static and wave moments are calculated, and the ship structures are optimized to resist the water corrosion by multi-island genetic algorithm. Deterministic design results from the RDM and IDM are compared with each separate robust design result. The proposed method shows great efficiency and accuracy.展开更多
The paper presents a knowledge-based engineering (KBE) approach for ship node components design. In the ship design process, many design tasks need design experiences to support. Howev- er, a ship design process is ...The paper presents a knowledge-based engineering (KBE) approach for ship node components design. In the ship design process, many design tasks need design experiences to support. Howev- er, a ship design process is a complicated process with many simultaneously repetitive and time-con- suming activities. In this research, the method combines KBE with Tribon system's built-in devel- opment language tools of Vitesse, captures and applies design knowledge for achieving standard com- ponents intelligent design modeling. A case study and industry implementation illustrate the feasibili- ty of the proposed methodology. The KBE technique can provide not only proper references, sug- gests and supports but also knowledge integrated in the ship structure design. Especially, these rules related to the design can avoid lots of design mistakes. During the ship design stage, getting more precise and better designs will not only reduce the time of rework and wasting resources but also shorten the construction time_ imnrov~ clilnl;hz ~nA nrnf;t展开更多
Development and application of a prototype KBE system is presented, details of the development tools and platforms, system flow chart, hybrid knowledge representation, and integrated system framework are illustrated. ...Development and application of a prototype KBE system is presented, details of the development tools and platforms, system flow chart, hybrid knowledge representation, and integrated system framework are illustrated. All design tasks of a missile seeker are integrated into a single computer-aided environment with a clear guidance to design processes from the user interface.展开更多
The agility of Internet of Things(IoT)software engineering is benchmarked based on its systematic insights for wide application support infrastructure developments.Such developments are focused on reducing the interfa...The agility of Internet of Things(IoT)software engineering is benchmarked based on its systematic insights for wide application support infrastructure developments.Such developments are focused on reducing the interfacing complexity with heterogeneous devices through applications.To handle the interfacing complexity problem,this article introduces a Semantic Interfacing Obscuration Model(SIOM)for IoT software-engineered platforms.The interfacing obscuration between heterogeneous devices and application interfaces from the testing to real-time validations is accounted for in this model.Based on the level of obscuration between the infrastructure hardware to the end-user software,the modifications through device replacement,capacity amendments,or interface bug fixes are performed.These modifications are based on the level of semantic obscurations observed during the application service intervals.The obscuration level is determined using knowledge learning as a progression from hardware to software semantics.The results reported were computed using specific metrics obtained from these experimental evaluations:an 8.94%reduction in interfacing complexity and a 15.04%improvement in integration progression.The knowledge of obscurationsmaps themodifications appropriately to reinstate the agility testing of the hardware/software integrations.This modification-based semantics is verified using semantics error,modification time,and complexity.展开更多
Patchoulol is a widely used sesquiterpenoid in perfumes,cosmetics,foods and pharmaceuticals.The plant-dependent production is suffering from limited growing area,long seasonal cycle,etc.Microbial production represents...Patchoulol is a widely used sesquiterpenoid in perfumes,cosmetics,foods and pharmaceuticals.The plant-dependent production is suffering from limited growing area,long seasonal cycle,etc.Microbial production represents a sustainable alternative as it is featured with mild operating conditions and eco-friendliness.Herein,we engineered the oleaginous Rhodotorula toruloides toward patchoulol production.First,the patchoulol biosynthesis baseline was constructed by employing a chimeric enzyme of the Pogostemon cablin originated patchoulol synthase and the native FPPS.Second,the supply of essential intermediates was streamlined by redeploying the mevalonate(MVA)pathway while the recycling of NADPH was enhanced through over-expressing related enzymes.Third,the patchoulol production was further enhanced to 724.8 mg/L,6.0 mg/L/h and 36.2 mg/g glucose by down-regulating the squalene biosynthesis and tuning the cultivation condition in shake flask.Finally,the production of patchoulol was increased to 1.31 g/L and 13.8 mg/g glucose in the minimal medium in a 3-L bioreactor.Our study demonstrated the potential of R.toruloides in producing patchoulol,and should shed light on the microbial synthesis of other sesquiterpenes.展开更多
Carbon-based substrates in Zn-MnO2flexible batteries have issues of low adhesion to MnO2,impacting cycle stability and capacity performance.A triple-synergistic strategy integrating C-O-Mn covalent bonding,wetta...Carbon-based substrates in Zn-MnO2flexible batteries have issues of low adhesion to MnO2,impacting cycle stability and capacity performance.A triple-synergistic strategy integrating C-O-Mn covalent bonding,wettability optimization,and hierarchical mesoporous engineering via cellulose nanofibers/carbon nanotube(CNF/CNT)-modified carbon cloth(CC)was proposed.This design achieves a“surface-locking”effect between the substrate and electrode materials,which was proven through theory and experiments.Density functional theory(DFT)simulations validate the“surface-locking”mechanism,where oxygen functionalities on CNF can form robust CO-Mn bonds with MnO2,inducing an increase in MnO2adsorption energy from-0.21 e V(pristine CC)to-1.36 e V,effectively suppressing Mn dissolution.Optimal wettability(contact angle:97°)reduced Zn2+desolvation and water-induced side reactions.Hierarchical pore structures accelerated Zn2+diffusion.The optimized CC@CNF1/CNT2-MnO2cathode achieves 92%capacity retention after 2000 cycles at 1 A/g.This study highlights a surface engineering strategy that effectively addresses the individual challenges associated with interfacial adhesion,reaction kinetics,and ion transport.This strategy offers fundamental insights into electrode interface modification for the development of nextgeneration flexible energy storage systems.展开更多
A growing global population and the increasing prevalence of diet-related health issues such as“hidden hunger”,obesity,hypertension,and diabetes necessitate a fundamental rethinking of crop design and breeding.Synth...A growing global population and the increasing prevalence of diet-related health issues such as“hidden hunger”,obesity,hypertension,and diabetes necessitate a fundamental rethinking of crop design and breeding.Synthetic metabolic engineering offers a method to modify and redesign metabolic pathways to increase the nutritional value of crops.We summarize recent advances in the biofortification of key nutrients including provitamin A,vitamin C,vitamin B9,iron,zinc,anthocyanins,flavonoids,and unsaturated fatty acids.We discuss the potential of multi-gene stacking,gene editing,enzyme engineering,and artificial intelligence in synthetic metabolic engineering.We propose future research directions and potential solutions centered on leveraging AI-driven systems biology,precision gene editing,enzyme engineering,agrobacterium-mediated genotype-independent transformation,and modular metabolic engineering strategies to develop next-generation nutritionally enhanced super crops and transform global food systems.展开更多
Electrocatalysis stands as a cornerstone in the pursuit of clean energy conversion and environmental sustainability,with single-atom catalysts(SACs)emerging as a transformative paradigm for enhancing electrocatalytic ...Electrocatalysis stands as a cornerstone in the pursuit of clean energy conversion and environmental sustainability,with single-atom catalysts(SACs)emerging as a transformative paradigm for enhancing electrocatalytic efficiency.In the architectural design of SACs,supports transcend conventional roles as mere supports,actively governing catalytic performance via robust metal-support interactions(SMSI).This review comprehensively analyses the key role of support engineering in modulating SACs performance.The study begins with a systematic assessment of currently popular SACs synthesis strategies,critically comparing their advantages and limitations.Through a hierarchical analysis,it reveals the impact of various support materials,such as carbon-based materials,metal oxides,MXenes,and metal-organic frameworks(MOFs),on the catalytic performance of SACs,with emphasis on their structural characteristics,electronic properties,and interaction mechanisms with active sites.The review further explores applications in energy conversion/storage and environmental remediation,while addressing current challenges and proposing future research directions for SACs development.By providing actionable insights,this work aims to guide the design of next-generation SACs and advance sustainable electrocatalysis.展开更多
Plants produce a vast array of specialized metabolites that serve as essential defenses against herbivores and pathogens.However,the capacity to produce these compounds differs substantially among plant species and is...Plants produce a vast array of specialized metabolites that serve as essential defenses against herbivores and pathogens.However,the capacity to produce these compounds differs substantially among plant species and is frequently diminished during domestication.Advances in synthetic metabolic engineering enable efficient elucidation and engineering of plant specialized metabolic pathways active in crop pest and pathogen resistance.This review summarizes strategies and workflows for selecting defensive metabolic pathways,identifying candidate biosynthetic genes,and rewiring native or introducing heterologous pathways to enhance crop resistance to pests and pathogens.Strategies include weighted gene co-expression network construction,biosynthetic gene cluster scanning,and metabolite genome-wide association studies for pathway discovery,as well as transcriptional reprogramming,enzyme activity optimization,and transporter deployment for pathway engineering.We further discuss challenges in using synthetic metabolic engineering to enhance crop resistance and highlight the potential of artificial intelligence in addressing them.展开更多
l-Isoleucine(L-Ile),a critical branched-chain amino acid with diverse applications in food,pharmaceutical,and cosmetic industries,is difficult to produce efficiently at scale in microbial systems due to metabolic bott...l-Isoleucine(L-Ile),a critical branched-chain amino acid with diverse applications in food,pharmaceutical,and cosmetic industries,is difficult to produce efficiently at scale in microbial systems due to metabolic bottlenecks and cofactor limitations.This study metabolically engineered Escherichia coli BL21(DE3)to develop a whole-cell biocatalyst for efficient L-Ile biosynthesis.Key strategies included screening acetohydroxy acid synthase(AHAS)isoenzymes,identifying ilvGM-encoded AHAS II as the optimal enzyme,relieving feedback inhibition of ilvA(encoding l-threonine dehydratase)through mutant screening,and optimizing genetic circuits(promoter tuning,plasmid copy number).Dual-precursor supplementation revealed l-threonine as a critical factor for suppressing l-valine byproduct.Fed-batch fermentation in a 5 L bioreactor achieved a peak molar conversion rate of 98.4%,yielding 40.1 g/L L-Ile within 36 h.The mass conversion rate(L-Ile/glucose)achieved 0.36 g/g and the production efficiency achieved 1.11 g/L/h,demonstrating the feasibility of whole-cell catalysis.This work provides a robust framework for industrial L-Ile production and transferable strategies for branched-chain amino acid pathway optimization.展开更多
Ergosterol is the key precursor of steroid drug synthesis.In this experiment,we systematically modified the synthesis of ergosterol.Firstly,we identified key rate-limiting enzymes through systematic screening of the p...Ergosterol is the key precursor of steroid drug synthesis.In this experiment,we systematically modified the synthesis of ergosterol.Firstly,we identified key rate-limiting enzymes through systematic screening of the post-squalene pathway.Combinatorial overexpression of IDI1,tHMG1,ERG4,ERG5,ERG27,ERG1 and ERG11 achieved an ergosterol titer of 94.2 mg/L.Molecular dynamics guided mutagenesis of key substrate channel residues,particularly S372V Erg11,enhanced local flexibility and significantly increased ergosterol production.Introduction of the proton-donating mutations S372V-T305H-ERG11 established an artificial proton-dependent pathway,which,together with channel engineering,further increased the titer to 124 mg/L.Lipid droplet engineering and cellular compartmentalization strategies increased the titer to 148.3 mg/L.Ultimately,multi-copy integration of all ergosterol synthesis pathway genes increased the titer to 433.1 mg/L,and fed-batch fermentation in a 5-L bioreactor resulted in a final titer of 4.58 g/L.This study demonstrates a comprehensive hierarchical strategy for high-level sterol production.展开更多
This article proposes an optimized strategy integrating bimetallic doping with interfacial wettability engineering to successfully fabricate a Ru-Co bimetallic-doped MoS2 catalyst vertically oriented on modified ca...This article proposes an optimized strategy integrating bimetallic doping with interfacial wettability engineering to successfully fabricate a Ru-Co bimetallic-doped MoS2 catalyst vertically oriented on modified carbon cloth for efficient hydrogen evolution reaction.The treated carbon cloth not only enhances its surface hydrophilicity but also provides nucleation sites for the growth of RuCo-MoS2 nanosheets.Subsequently,the development of an oriented growth induction strategy enables the vertical alignment of bimetallic atom-doped MoS2 on modified carbon cloth.This vertically grown structure is conducive to exposing more active sites,shortening the proton transport path,reducing the charge transfer impedance.Moreover,this study employs a reductive bonding technique to precisely modulate the coordination environments and electron distributions of co-doped Co and Ru bimetallic atoms,as well as significantly improving the hydrogen evolution reaction kinetics.Therefore,the as-prepared RuCo-MoS2/MCC catalyst demonstrates excellent HER performance in acidic electrolyte,exhibiting a relatively low overpotential of 62 mV at 10 mA·cm-2and a small Tafel slope of 48.2 mV·dec-1.展开更多
Pitch-derived hard carbons(HC)are promising anodes for sodium-ion batteries(SIBs)due to their high carbonization yield and low cost.However,the inherent compositional heterogeneity of pitch induces non-uniform oxidati...Pitch-derived hard carbons(HC)are promising anodes for sodium-ion batteries(SIBs)due to their high carbonization yield and low cost.However,the inherent compositional heterogeneity of pitch induces non-uniform oxidative cross-linking during conventional pre-oxidation,which not only renders the microstructure of HC difficult to regulate but also significantly degrades its sodium storage performance.Here,we identify the“shielding effect”of oxidation-inert components in pitch as the root cause of this structural inhomogeneity.To overcome this limitation,we propose a novel“sieving-and-reinforcement strategy”.This involves liquid-phase crosslinking to construct a polar three-dimensional(3D)carbon skeleton,followed by stepwise extraction as a molecular sieving process to remove inert components and expose the reactive skeleton,and finally,oxygen etching as a reinforcement step to drastically enhance the crosslinking density and defect population.This controllably engineered carbon skeleton in-situ evolves into an HC with a uniform hierarchical porous structure,featuring abundant ultramicropores,optimally sized closed pores(-2.15 nm),and ultrathin pore walls during carbonization.The resulting HC anode delivers a high reversible capacity of 363.3 mAh g-1at 50 mA g-1,with an impressive plateau capacity contribution of 71.5%.It also demonstrates exceptional cycling stability,retaining203.1 mAh g-1after 500 cycles at a high current density of 1000 mA g-1.This work provides a fundamental understanding of precursor engineering,paving the way for the rational design of advanced carbon materials for next-generation energy storage.展开更多
This paper systematically reviews the development stages and current status of key oil production engineering domains,including injection-production engineering,artificial lift,reservoir stimulation,and workover opera...This paper systematically reviews the development stages and current status of key oil production engineering domains,including injection-production engineering,artificial lift,reservoir stimulation,and workover operations.The major challenges for oil production engineering are identified in four aspects:intelligent terminal equipment and process integration,extreme-environment operations,and collaborative operational constraints;AI-driven data and modeling complexities,and advanced structural and functional materials requirements;and the need for geology-engineering integration in reservoir characterization,operational efficiency and green development.Centered on multidisciplinary integration,the concept of the Oil Production Engineering Agent is introduced as a miniaturized,intelligent,and integrated hardware-software system designed for extreme downhole environments and complex conditions,incorporating power supply,communication,sensing,computation,and actuation modules to enable environmental perception,autonomous decision-making and adaptive control.The characteristics of various agent types,including those for injection-production,lift,fracturing and workover,are analyzed,with key research directions identified in miniaturized self-powered energy management,reliable communication in high-interference environments,highly integrated multi-parameter sensing with long-term drift self-calibration,and high-reliability microsystem integration manufacturing.AI-driven decision optimization remains the core feature,requiring advances in data acquisition,governance,and fusion architectures,alongside algorithmic improvements in model performance and deployment compatibility.Additionally,advanced structural and functional materials support agent construction and extreme-environment adaptability,while geology-engineering integration continues to expand the functional scope of oil production engineering.展开更多
As a new wave of scientific and technological revolution gathers force,China and the world are entering a new era of ecological civilization shaped by both unprecedented opportunities and profound challenges.In such a...As a new wave of scientific and technological revolution gathers force,China and the world are entering a new era of ecological civilization shaped by both unprecedented opportunities and profound challenges.In such a moment,the renewal of a journal is not merely an editorial decision.It is also an intellectual and institutional response to the questions of the age.展开更多
The exposure of specific facets in catalysts plays a pivotal role in surface/interface reactions.This study systematically explores facet engineering as a novel approach to enhancing the piezoelectric and piezo-photoc...The exposure of specific facets in catalysts plays a pivotal role in surface/interface reactions.This study systematically explores facet engineering as a novel approach to enhancing the piezoelectric and piezo-photocatalytic capabilities of metal-organic frameworks(MOFs),with a focus on ZIF-8 as a model compound.By selectively exposing specific facets-(100),(110),and a combination of both in mixed configurations,this research examines how facet orientation affects piezoelectric properties,charge separation efficiency,and catalytic performance.The ZIF-8 samples,identified as ZIF-8-RD,ZIF-8-CUBE,ZIF-8-TRD_1,and ZIF-8-TRD2 demonstrated distinct catalytic activities in photocatalysis,piezocatalysis,and piezo-photocatalysis.Notably,ZIF-8-TRDs,with the mixed-facet exposure,showed superior catalytic performance,achieving up to 94%degradation of tetracycline(TC)in piezo-photocatalysis,a substantial improvement over the single-facet variant.This enhanced performance is attributed to the mixed facets'higher carrier concentration and superior charge separation facilitated by the increased internal piezoelectric potential.Density functional theory(DFT)calculations corroborate the experimental results,revealing that mixed facets contribute to a larger dipole moment,indicating greater structural asymmetry and piezoelectric efficiency.The findings underscore facet engineering as an effective strategy to optimize MOF-based catalysts,opening avenues for high-performance materials tailored for environmental remediation and sustainable energy applications.This work not only pioneers facet engineering in MOF piezophotocatalysts but also opens new avenues for the development and enhancement of high-performance MOF in piezoelectricity.展开更多
Additive manufacturing,or 3D printing,has transformed tissue engineering by enabling the creation of intricate scaffolds and cell scaffold constructs that closely mimic organic tissues.This technique provides a founda...Additive manufacturing,or 3D printing,has transformed tissue engineering by enabling the creation of intricate scaffolds and cell scaffold constructs that closely mimic organic tissues.This technique provides a foundation for creating structures that promote cell proliferation and tissue growth.The shift from 3D to 4D printing has introduced dynamic and time-responsive scaffolds that enhance the capabilities of tissue engineering by allowing constructs to modify their shape or function in response to environmental stimuli.These advancements are crucial for formulating adaptable treatments for human tissue regeneration.Bioprinting,a branch of 3D and 4D printing,incorporates living cells into printed constructs,resulting in cell-infused entities that harmoniously integrate with the human body.This method has considerable promise for producing live tissues;yet,both 3D/4D printing and bioprinting have technological limitations concerning precision,scalability,and functional intricacy.In response to these challenges,5D printing has emerged as a conceptual innovation beyond traditional dimensions.In 5D bioprinting,information serves as the fifth dimension and is embedded into printed objects along the dimensions of space and time.This supplementary information enables 5D-printed structures to interact more dynamically with their surroundings.Upon activation,this embedded information can alter ambient conditions or trigger functional responses inside printed objects,thereby enhancing the adaptability and integration with biological systems.This article presents a comprehensive analysis of 4D bioprinting methodologies in tissue engineering,followed by an exploration of the potential of 5D printing.5D bioprinting incorporates spatial,temporal,and informational dimensions,fostering innovative prospects in biomedical applications,and advancing the creation of intelligent scaffolds and adaptive biomaterials that can transform regenerative medicine and personalized healthcare.展开更多
Nd-Fe-B sintered magnets,critical for enhancing electromechanical conversion efficiency and operational stability in wind turbines and electric vehicle drives,are prized for their outstanding coercivity,high remanence...Nd-Fe-B sintered magnets,critical for enhancing electromechanical conversion efficiency and operational stability in wind turbines and electric vehicle drives,are prized for their outstanding coercivity,high remanence,and superior maximum energy product.However,the coercivity-remanence trade-off,combined with severe imbalances in rare-earth resource utilization,presents two fundamental bottlenecks hindering the development of cost-effective high-performance Nd-Fe-B sintered magnets.To overcome these limitations,significant research efforts are focused on the in-depth exploration,optimization,and innovative design of microstructures.This review synthesizes the world-wide advances and the author group's latest findings in multidimensional microstructural engineering,anchored in the characteristic microstructural features formed via powder metallurgy technology.It primarily examines the technical strategies,underlying mechanisms and economic evaluation across grain size and morphology optimization,spatial distribution control of the Nd6Fe13Ga phase,grain boundary diffusion sources iteration and process improvements,advancements in dual main-phase Ce magnets,and unique gradient structure design.The persistent challenges for future Nd-Fe-B sintered magnets development and the emerging role of machine learning in guiding technological process,performance prediction,microstructure characterization,and novel permanent magnets discovery are also highlighted.展开更多
The rapid advancement of aerospace and electronic information technologies has imposed increasingly stringent requirements on microwave absorbing materials(MAMs),such as high absorption efficiency,lightweight,and envi...The rapid advancement of aerospace and electronic information technologies has imposed increasingly stringent requirements on microwave absorbing materials(MAMs),such as high absorption efficiency,lightweight,and environmental stability,making the development of advanced MAMs urgent for both civilian and national defense applications.MXenes,as an emerging two-dimensional material,exhibit great potential as MAMs due to their tunable surface chemistry,excellent conductivity,and diverse composite properties.However,existing reviews of MXene-based MAMs lack a systematic overview of the synergistic mechanisms between MXenes and other novel materials as well as composition and structure synergistic regulation strategies for performance optimization.This work comprehensively reviews the latest research progress on MXene-based MAMs,first elaborating on their various loss mechanisms,including conductive loss,polarization loss,and magnetic loss.Furthermore,various composite strategies(hybridization with carbon-based,magnetic,polymeric,and ceramic materials)and their synergistic effects are explored together with the impact of structural engineering(0D/1D/2D/3D,heterostructures,porous structures)on the tuning of electromagnetic wave absorption performance.Finally,this work discusses the current challenges and future development directions of MXene-based MAMs,aiming to establish composition-structure-function correlations and provide a reference for their future development.展开更多
基金financially supported by the Project of Ministry of Education and Finance of China(Grant Nos.200512 and 201335)the Project of the State Key Laboratory of Ocean Engineering,Shanghai Jiao Tong University(Grant No.GKZD010053-10)
摘要Knowledge-Based Engineering (KBE) is introduced into the ship structural design in this paper. From the implementation of KBE, the design solutions for both Rules Design Method (RDM) and Interpolation Design Method (IDM) are generated. The corresponding Finite Element (FE) models are generated. Topological design of the longitudinal structures is studied where the Gaussian Process (GP) is employed to build the surrogate model for FE analysis. Multi-objective optimization methods inspired by Pareto Front are used to reduce the design tank weight and outer surface area simultaneously. Additionally, an enhanced Level Set Method (LSM) which employs implicit algorithm is applied to the topological design of typical bracket plate which is used extensively in ship structures. Two different sets of boundary conditions are considered. The proposed methods show satisfactory efficiency and accuracy.
基金the Project of Ministry of Finance andMinistry of Education of China(Nos.200512 and201335)the State Key Laboratory of Ocean Engineering Foundation of Shanghai Jiao Tong University(No.GKZD010053-10)
摘要Knowledge-based engineering(KBE) has made success in automobile and molding design industry, and it is introduced into the ship structural design in this paper. From the implementation of KBE, the deterministic design solutions for both rules design method(RDM) and interpolation design method(IDM) are generated. The corresponding finite element model is generated. Gaussian process(GP) is then employed to build the surrogate model for finite element analysis, in order to increase efficiency and maintain accuracy at the same time, and the multi-modal adaptive importance sampling method is adopted to calculate the corresponding structural reliability.An example is given to validate the proposed method. Finally, the reliabilities of the structures' strength caused by uncertainty lying in water corrosion, static and wave moments are calculated, and the ship structures are optimized to resist the water corrosion by multi-island genetic algorithm. Deterministic design results from the RDM and IDM are compared with each separate robust design result. The proposed method shows great efficiency and accuracy.
基金Supported by the'Knowledge-based Ship-design Hyper-integrated Platform(KSHIP)'of Ministry of Education and Finance of P.R.China(No.200512)the National Natural Science Foundation of China(No.51009093)
摘要The paper presents a knowledge-based engineering (KBE) approach for ship node components design. In the ship design process, many design tasks need design experiences to support. Howev- er, a ship design process is a complicated process with many simultaneously repetitive and time-con- suming activities. In this research, the method combines KBE with Tribon system's built-in devel- opment language tools of Vitesse, captures and applies design knowledge for achieving standard com- ponents intelligent design modeling. A case study and industry implementation illustrate the feasibili- ty of the proposed methodology. The KBE technique can provide not only proper references, sug- gests and supports but also knowledge integrated in the ship structure design. Especially, these rules related to the design can avoid lots of design mistakes. During the ship design stage, getting more precise and better designs will not only reduce the time of rework and wasting resources but also shorten the construction time_ imnrov~ clilnl;hz ~nA nrnf;t
基金Supported by the National High-Tech. R&D Program (863 program) for CIMS(2003AA411350)
摘要Development and application of a prototype KBE system is presented, details of the development tools and platforms, system flow chart, hybrid knowledge representation, and integrated system framework are illustrated. All design tasks of a missile seeker are integrated into a single computer-aided environment with a clear guidance to design processes from the user interface.
摘要The agility of Internet of Things(IoT)software engineering is benchmarked based on its systematic insights for wide application support infrastructure developments.Such developments are focused on reducing the interfacing complexity with heterogeneous devices through applications.To handle the interfacing complexity problem,this article introduces a Semantic Interfacing Obscuration Model(SIOM)for IoT software-engineered platforms.The interfacing obscuration between heterogeneous devices and application interfaces from the testing to real-time validations is accounted for in this model.Based on the level of obscuration between the infrastructure hardware to the end-user software,the modifications through device replacement,capacity amendments,or interface bug fixes are performed.These modifications are based on the level of semantic obscurations observed during the application service intervals.The obscuration level is determined using knowledge learning as a progression from hardware to software semantics.The results reported were computed using specific metrics obtained from these experimental evaluations:an 8.94%reduction in interfacing complexity and a 15.04%improvement in integration progression.The knowledge of obscurationsmaps themodifications appropriately to reinstate the agility testing of the hardware/software integrations.This modification-based semantics is verified using semantics error,modification time,and complexity.
基金supported by National Natural Science Foundation of China(21602218 to X.B.Y.)Chinese Universities Scientific Fund(2452018314 to X.B.Y.)+1 种基金the Key project at the central government level:the ability establishment of sustainable use for valuable Chinese medicine resources(2060302 to X.B.Y.)the Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(CI2024-C008YNL to Y.N.W.).
摘要Patchoulol is a widely used sesquiterpenoid in perfumes,cosmetics,foods and pharmaceuticals.The plant-dependent production is suffering from limited growing area,long seasonal cycle,etc.Microbial production represents a sustainable alternative as it is featured with mild operating conditions and eco-friendliness.Herein,we engineered the oleaginous Rhodotorula toruloides toward patchoulol production.First,the patchoulol biosynthesis baseline was constructed by employing a chimeric enzyme of the Pogostemon cablin originated patchoulol synthase and the native FPPS.Second,the supply of essential intermediates was streamlined by redeploying the mevalonate(MVA)pathway while the recycling of NADPH was enhanced through over-expressing related enzymes.Third,the patchoulol production was further enhanced to 724.8 mg/L,6.0 mg/L/h and 36.2 mg/g glucose by down-regulating the squalene biosynthesis and tuning the cultivation condition in shake flask.Finally,the production of patchoulol was increased to 1.31 g/L and 13.8 mg/g glucose in the minimal medium in a 3-L bioreactor.Our study demonstrated the potential of R.toruloides in producing patchoulol,and should shed light on the microbial synthesis of other sesquiterpenes.
基金supported by the National Natural Science Foundation of China(32401531,32301530,32271814)the Innovation Project of Excellent Doctoral Dissertation of Tianjin University of Science and Technology(YB2023004)+4 种基金the China Scholarship Council(No.202408120105,202308120079,202208120049)the Young Elite Scientist Sponsorship Program by Cast(No.YESS20230242)the Natural Science Foundation of Tianjin(24JCZDJC00630,23JCZDJC00630)Guangxi Key Technologies R&D Program“Research and demonstration of key technologies for preparing high-performance wood-based panels from agricultural and forestry residues”under Grant No.AB23026096the Tianjin Enterprise Technology Commissioner Project(25YDTPJC00690)。
摘要Carbon-based substrates in Zn-MnO2flexible batteries have issues of low adhesion to MnO2,impacting cycle stability and capacity performance.A triple-synergistic strategy integrating C-O-Mn covalent bonding,wettability optimization,and hierarchical mesoporous engineering via cellulose nanofibers/carbon nanotube(CNF/CNT)-modified carbon cloth(CC)was proposed.This design achieves a“surface-locking”effect between the substrate and electrode materials,which was proven through theory and experiments.Density functional theory(DFT)simulations validate the“surface-locking”mechanism,where oxygen functionalities on CNF can form robust CO-Mn bonds with MnO2,inducing an increase in MnO2adsorption energy from-0.21 e V(pristine CC)to-1.36 e V,effectively suppressing Mn dissolution.Optimal wettability(contact angle:97°)reduced Zn2+desolvation and water-induced side reactions.Hierarchical pore structures accelerated Zn2+diffusion.The optimized CC@CNF1/CNT2-MnO2cathode achieves 92%capacity retention after 2000 cycles at 1 A/g.This study highlights a surface engineering strategy that effectively addresses the individual challenges associated with interfacial adhesion,reaction kinetics,and ion transport.This strategy offers fundamental insights into electrode interface modification for the development of nextgeneration flexible energy storage systems.
基金supported by grants from the Guangxi Science and Technology Major Project(GKAA24206023)the Biological Breeding-National Science and Technology Major Project(2024ZD04077)+2 种基金the National Natural Science Foundation of China(32272120)the National Key Research and Development Program of China(2024YFF1000800)the Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops Major Project(FCBRCE-202502,FCBRCE-202504).
摘要A growing global population and the increasing prevalence of diet-related health issues such as“hidden hunger”,obesity,hypertension,and diabetes necessitate a fundamental rethinking of crop design and breeding.Synthetic metabolic engineering offers a method to modify and redesign metabolic pathways to increase the nutritional value of crops.We summarize recent advances in the biofortification of key nutrients including provitamin A,vitamin C,vitamin B9,iron,zinc,anthocyanins,flavonoids,and unsaturated fatty acids.We discuss the potential of multi-gene stacking,gene editing,enzyme engineering,and artificial intelligence in synthetic metabolic engineering.We propose future research directions and potential solutions centered on leveraging AI-driven systems biology,precision gene editing,enzyme engineering,agrobacterium-mediated genotype-independent transformation,and modular metabolic engineering strategies to develop next-generation nutritionally enhanced super crops and transform global food systems.
基金financially supported by the Guangxi Natural Science Fund for Distinguished Young Scholars(No.2024GXNSFFA010008)the Special Fund for Science and Technology Development of Guangxi(No.AD25069078)the National Natural Science Foundation of China(No.22469002)。
摘要Electrocatalysis stands as a cornerstone in the pursuit of clean energy conversion and environmental sustainability,with single-atom catalysts(SACs)emerging as a transformative paradigm for enhancing electrocatalytic efficiency.In the architectural design of SACs,supports transcend conventional roles as mere supports,actively governing catalytic performance via robust metal-support interactions(SMSI).This review comprehensively analyses the key role of support engineering in modulating SACs performance.The study begins with a systematic assessment of currently popular SACs synthesis strategies,critically comparing their advantages and limitations.Through a hierarchical analysis,it reveals the impact of various support materials,such as carbon-based materials,metal oxides,MXenes,and metal-organic frameworks(MOFs),on the catalytic performance of SACs,with emphasis on their structural characteristics,electronic properties,and interaction mechanisms with active sites.The review further explores applications in energy conversion/storage and environmental remediation,while addressing current challenges and proposing future research directions for SACs development.By providing actionable insights,this work aims to guide the design of next-generation SACs and advance sustainable electrocatalysis.
基金supported by the National Natural Science Foundation of China (32402306)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences+1 种基金National Key Research and Development Program of China (2022YFE0203300)the China-Uruguay Joint Laboratory on Soybean Research and Innovation
摘要Plants produce a vast array of specialized metabolites that serve as essential defenses against herbivores and pathogens.However,the capacity to produce these compounds differs substantially among plant species and is frequently diminished during domestication.Advances in synthetic metabolic engineering enable efficient elucidation and engineering of plant specialized metabolic pathways active in crop pest and pathogen resistance.This review summarizes strategies and workflows for selecting defensive metabolic pathways,identifying candidate biosynthetic genes,and rewiring native or introducing heterologous pathways to enhance crop resistance to pests and pathogens.Strategies include weighted gene co-expression network construction,biosynthetic gene cluster scanning,and metabolite genome-wide association studies for pathway discovery,as well as transcriptional reprogramming,enzyme activity optimization,and transporter deployment for pathway engineering.We further discuss challenges in using synthetic metabolic engineering to enhance crop resistance and highlight the potential of artificial intelligence in addressing them.
基金supported by the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(32021005)National First-class Discipline Program of Light Industry Technology and Engineering(QGJC20230102).
摘要l-Isoleucine(L-Ile),a critical branched-chain amino acid with diverse applications in food,pharmaceutical,and cosmetic industries,is difficult to produce efficiently at scale in microbial systems due to metabolic bottlenecks and cofactor limitations.This study metabolically engineered Escherichia coli BL21(DE3)to develop a whole-cell biocatalyst for efficient L-Ile biosynthesis.Key strategies included screening acetohydroxy acid synthase(AHAS)isoenzymes,identifying ilvGM-encoded AHAS II as the optimal enzyme,relieving feedback inhibition of ilvA(encoding l-threonine dehydratase)through mutant screening,and optimizing genetic circuits(promoter tuning,plasmid copy number).Dual-precursor supplementation revealed l-threonine as a critical factor for suppressing l-valine byproduct.Fed-batch fermentation in a 5 L bioreactor achieved a peak molar conversion rate of 98.4%,yielding 40.1 g/L L-Ile within 36 h.The mass conversion rate(L-Ile/glucose)achieved 0.36 g/g and the production efficiency achieved 1.11 g/L/h,demonstrating the feasibility of whole-cell catalysis.This work provides a robust framework for industrial L-Ile production and transferable strategies for branched-chain amino acid pathway optimization.
基金supported by The National Key Research and Development Program of China(2024YFA0919900)The National Natural Science Foundation of China(22578175).
摘要Ergosterol is the key precursor of steroid drug synthesis.In this experiment,we systematically modified the synthesis of ergosterol.Firstly,we identified key rate-limiting enzymes through systematic screening of the post-squalene pathway.Combinatorial overexpression of IDI1,tHMG1,ERG4,ERG5,ERG27,ERG1 and ERG11 achieved an ergosterol titer of 94.2 mg/L.Molecular dynamics guided mutagenesis of key substrate channel residues,particularly S372V Erg11,enhanced local flexibility and significantly increased ergosterol production.Introduction of the proton-donating mutations S372V-T305H-ERG11 established an artificial proton-dependent pathway,which,together with channel engineering,further increased the titer to 124 mg/L.Lipid droplet engineering and cellular compartmentalization strategies increased the titer to 148.3 mg/L.Ultimately,multi-copy integration of all ergosterol synthesis pathway genes increased the titer to 433.1 mg/L,and fed-batch fermentation in a 5-L bioreactor resulted in a final titer of 4.58 g/L.This study demonstrates a comprehensive hierarchical strategy for high-level sterol production.
基金the financial support of the National Natural Science Foundation of China(22108145)State Key Laboratory of Heavy Oil Processing(SKLHOP202203008)the Outstanding Young Innovation Teams of Colleges and Universities in Shandong Province(2023KJC016)。
摘要This article proposes an optimized strategy integrating bimetallic doping with interfacial wettability engineering to successfully fabricate a Ru-Co bimetallic-doped MoS2 catalyst vertically oriented on modified carbon cloth for efficient hydrogen evolution reaction.The treated carbon cloth not only enhances its surface hydrophilicity but also provides nucleation sites for the growth of RuCo-MoS2 nanosheets.Subsequently,the development of an oriented growth induction strategy enables the vertical alignment of bimetallic atom-doped MoS2 on modified carbon cloth.This vertically grown structure is conducive to exposing more active sites,shortening the proton transport path,reducing the charge transfer impedance.Moreover,this study employs a reductive bonding technique to precisely modulate the coordination environments and electron distributions of co-doped Co and Ru bimetallic atoms,as well as significantly improving the hydrogen evolution reaction kinetics.Therefore,the as-prepared RuCo-MoS2/MCC catalyst demonstrates excellent HER performance in acidic electrolyte,exhibiting a relatively low overpotential of 62 mV at 10 mA·cm-2and a small Tafel slope of 48.2 mV·dec-1.
基金financial support from the National Natural Science Foundation of China(22108309 and 22478433)the Postdoctoral Innovation Project in Shandong Province(SDCX-ZG-202203099)+2 种基金the Shandong Provincial Natural Science Foundation(ZR2023MB005)the Fundamental Research Fund for the Central Universities(No.24CX06047A)the Taishan Scholar Program of Shandong(No.ts20190919 and No.tsqn202312135)。
摘要Pitch-derived hard carbons(HC)are promising anodes for sodium-ion batteries(SIBs)due to their high carbonization yield and low cost.However,the inherent compositional heterogeneity of pitch induces non-uniform oxidative cross-linking during conventional pre-oxidation,which not only renders the microstructure of HC difficult to regulate but also significantly degrades its sodium storage performance.Here,we identify the“shielding effect”of oxidation-inert components in pitch as the root cause of this structural inhomogeneity.To overcome this limitation,we propose a novel“sieving-and-reinforcement strategy”.This involves liquid-phase crosslinking to construct a polar three-dimensional(3D)carbon skeleton,followed by stepwise extraction as a molecular sieving process to remove inert components and expose the reactive skeleton,and finally,oxygen etching as a reinforcement step to drastically enhance the crosslinking density and defect population.This controllably engineered carbon skeleton in-situ evolves into an HC with a uniform hierarchical porous structure,featuring abundant ultramicropores,optimally sized closed pores(-2.15 nm),and ultrathin pore walls during carbonization.The resulting HC anode delivers a high reversible capacity of 363.3 mAh g-1at 50 mA g-1,with an impressive plateau capacity contribution of 71.5%.It also demonstrates exceptional cycling stability,retaining203.1 mAh g-1after 500 cycles at a high current density of 1000 mA g-1.This work provides a fundamental understanding of precursor engineering,paving the way for the rational design of advanced carbon materials for next-generation energy storage.
基金Supported by the National Key Research and Development Program Project(2024YFE0213100)China National Science and Technology Major Project(2024ZD1406500)+2 种基金General Program of the National Natural Science Foundation of China(52374067)Joint Fund Project of the National Natural Science Foundation of China(U25B20129)CNPC Prospective and Basic Technological Project(2023ZZ11).
摘要This paper systematically reviews the development stages and current status of key oil production engineering domains,including injection-production engineering,artificial lift,reservoir stimulation,and workover operations.The major challenges for oil production engineering are identified in four aspects:intelligent terminal equipment and process integration,extreme-environment operations,and collaborative operational constraints;AI-driven data and modeling complexities,and advanced structural and functional materials requirements;and the need for geology-engineering integration in reservoir characterization,operational efficiency and green development.Centered on multidisciplinary integration,the concept of the Oil Production Engineering Agent is introduced as a miniaturized,intelligent,and integrated hardware-software system designed for extreme downhole environments and complex conditions,incorporating power supply,communication,sensing,computation,and actuation modules to enable environmental perception,autonomous decision-making and adaptive control.The characteristics of various agent types,including those for injection-production,lift,fracturing and workover,are analyzed,with key research directions identified in miniaturized self-powered energy management,reliable communication in high-interference environments,highly integrated multi-parameter sensing with long-term drift self-calibration,and high-reliability microsystem integration manufacturing.AI-driven decision optimization remains the core feature,requiring advances in data acquisition,governance,and fusion architectures,alongside algorithmic improvements in model performance and deployment compatibility.Additionally,advanced structural and functional materials support agent construction and extreme-environment adaptability,while geology-engineering integration continues to expand the functional scope of oil production engineering.
摘要As a new wave of scientific and technological revolution gathers force,China and the world are entering a new era of ecological civilization shaped by both unprecedented opportunities and profound challenges.In such a moment,the renewal of a journal is not merely an editorial decision.It is also an intellectual and institutional response to the questions of the age.
基金supported by the National Natural Science Foundation of China(No.52302160)Beijing Municipal Education Commission(No.KM202310011007)+1 种基金the China Postdoctoral Science Foundation(No.2023M732522)the Jiangsu Funding Program for Excellent Postdoctoral Talent(No.2023ZB206)for financial support。
摘要The exposure of specific facets in catalysts plays a pivotal role in surface/interface reactions.This study systematically explores facet engineering as a novel approach to enhancing the piezoelectric and piezo-photocatalytic capabilities of metal-organic frameworks(MOFs),with a focus on ZIF-8 as a model compound.By selectively exposing specific facets-(100),(110),and a combination of both in mixed configurations,this research examines how facet orientation affects piezoelectric properties,charge separation efficiency,and catalytic performance.The ZIF-8 samples,identified as ZIF-8-RD,ZIF-8-CUBE,ZIF-8-TRD_1,and ZIF-8-TRD2 demonstrated distinct catalytic activities in photocatalysis,piezocatalysis,and piezo-photocatalysis.Notably,ZIF-8-TRDs,with the mixed-facet exposure,showed superior catalytic performance,achieving up to 94%degradation of tetracycline(TC)in piezo-photocatalysis,a substantial improvement over the single-facet variant.This enhanced performance is attributed to the mixed facets'higher carrier concentration and superior charge separation facilitated by the increased internal piezoelectric potential.Density functional theory(DFT)calculations corroborate the experimental results,revealing that mixed facets contribute to a larger dipole moment,indicating greater structural asymmetry and piezoelectric efficiency.The findings underscore facet engineering as an effective strategy to optimize MOF-based catalysts,opening avenues for high-performance materials tailored for environmental remediation and sustainable energy applications.This work not only pioneers facet engineering in MOF piezophotocatalysts but also opens new avenues for the development and enhancement of high-performance MOF in piezoelectricity.
摘要Additive manufacturing,or 3D printing,has transformed tissue engineering by enabling the creation of intricate scaffolds and cell scaffold constructs that closely mimic organic tissues.This technique provides a foundation for creating structures that promote cell proliferation and tissue growth.The shift from 3D to 4D printing has introduced dynamic and time-responsive scaffolds that enhance the capabilities of tissue engineering by allowing constructs to modify their shape or function in response to environmental stimuli.These advancements are crucial for formulating adaptable treatments for human tissue regeneration.Bioprinting,a branch of 3D and 4D printing,incorporates living cells into printed constructs,resulting in cell-infused entities that harmoniously integrate with the human body.This method has considerable promise for producing live tissues;yet,both 3D/4D printing and bioprinting have technological limitations concerning precision,scalability,and functional intricacy.In response to these challenges,5D printing has emerged as a conceptual innovation beyond traditional dimensions.In 5D bioprinting,information serves as the fifth dimension and is embedded into printed objects along the dimensions of space and time.This supplementary information enables 5D-printed structures to interact more dynamically with their surroundings.Upon activation,this embedded information can alter ambient conditions or trigger functional responses inside printed objects,thereby enhancing the adaptability and integration with biological systems.This article presents a comprehensive analysis of 4D bioprinting methodologies in tissue engineering,followed by an exploration of the potential of 5D printing.5D bioprinting incorporates spatial,temporal,and informational dimensions,fostering innovative prospects in biomedical applications,and advancing the creation of intelligent scaffolds and adaptive biomaterials that can transform regenerative medicine and personalized healthcare.
基金financially supported by the National Key Research and Development Program of China(Grant Nos.2024YFB3508800 and 2024YFB3508803)the High-quality Development Special Funds Program Ministry of Industry and Information Technology(Grant No.TC220H06G)the Inner Mongolia Autonomous Region Unveils Marshal Program from Rare Earth Advanced Materials Technology Innovation Center,and the Major Projects in Inner Mongolia Autonomous Region(Grant No.20212D0035).
摘要Nd-Fe-B sintered magnets,critical for enhancing electromechanical conversion efficiency and operational stability in wind turbines and electric vehicle drives,are prized for their outstanding coercivity,high remanence,and superior maximum energy product.However,the coercivity-remanence trade-off,combined with severe imbalances in rare-earth resource utilization,presents two fundamental bottlenecks hindering the development of cost-effective high-performance Nd-Fe-B sintered magnets.To overcome these limitations,significant research efforts are focused on the in-depth exploration,optimization,and innovative design of microstructures.This review synthesizes the world-wide advances and the author group's latest findings in multidimensional microstructural engineering,anchored in the characteristic microstructural features formed via powder metallurgy technology.It primarily examines the technical strategies,underlying mechanisms and economic evaluation across grain size and morphology optimization,spatial distribution control of the Nd6Fe13Ga phase,grain boundary diffusion sources iteration and process improvements,advancements in dual main-phase Ce magnets,and unique gradient structure design.The persistent challenges for future Nd-Fe-B sintered magnets development and the emerging role of machine learning in guiding technological process,performance prediction,microstructure characterization,and novel permanent magnets discovery are also highlighted.
基金financially supported by the project of the National Natural Science Foundation of China(Grant Nos.52402354,62174016,and 12374394)China Postdoctoral Science Foundation(Grant No.2023M740471)the Natural Science Foundation of Jiangsu Higher Education Institutions(Grant No.24KJB430002)。
摘要The rapid advancement of aerospace and electronic information technologies has imposed increasingly stringent requirements on microwave absorbing materials(MAMs),such as high absorption efficiency,lightweight,and environmental stability,making the development of advanced MAMs urgent for both civilian and national defense applications.MXenes,as an emerging two-dimensional material,exhibit great potential as MAMs due to their tunable surface chemistry,excellent conductivity,and diverse composite properties.However,existing reviews of MXene-based MAMs lack a systematic overview of the synergistic mechanisms between MXenes and other novel materials as well as composition and structure synergistic regulation strategies for performance optimization.This work comprehensively reviews the latest research progress on MXene-based MAMs,first elaborating on their various loss mechanisms,including conductive loss,polarization loss,and magnetic loss.Furthermore,various composite strategies(hybridization with carbon-based,magnetic,polymeric,and ceramic materials)and their synergistic effects are explored together with the impact of structural engineering(0D/1D/2D/3D,heterostructures,porous structures)on the tuning of electromagnetic wave absorption performance.Finally,this work discusses the current challenges and future development directions of MXene-based MAMs,aiming to establish composition-structure-function correlations and provide a reference for their future development.