A magnetic-fluorescent nanocomposite(CDs-MNPs)was synthesized via a facile one-pot microwave hy-drothermal method using polyethyleneimine(PEI)as a bridging agent.PEI connected magnetic nanoparticles(MNPs)and carbon do...A magnetic-fluorescent nanocomposite(CDs-MNPs)was synthesized via a facile one-pot microwave hy-drothermal method using polyethyleneimine(PEI)as a bridging agent.PEI connected magnetic nanoparticles(MNPs)and carbon dots(CDs),forming a fluffy clustered structure with nanoscale gaps,which effectively sup⁃pressed aggregation-induced photoluminescence quenching(AIQ).CDs-MNPs exhibited a saturation magnetization of 10.4 emu/g and stable solid-state green fluorescence.Benefiting from integrated magnetism,fluorescence,and strong adsorption to fingerprint residues,it achieved clear latent fingerprint(LFP)visualization on dark substrates.展开更多
Efficient,sensitive,and convenient quantum dots(QDs)fluorescent immunosensors have a wide range of applications in the field of in vitro diagnostics(IVD).However,traditional single-signal readout methods are susceptib...Efficient,sensitive,and convenient quantum dots(QDs)fluorescent immunosensors have a wide range of applications in the field of in vitro diagnostics(IVD).However,traditional single-signal readout methods are susceptible to interference,have limited sensitivity,and involve time-consuming detection processes.These have become key bottlenecks restricting their use in point-of-care(POC)detection.Herein,we developed a novel immunosensor based on QDs ratiometric fluorescence(QRF)and magnetic driven technology for sensitive,rapid,accurate detection of C-reactive protein(CRP).High-quality magnetic-fluorescent QDs nanobeads(QBs)were prepared by efficiently and orderly layered loading of magnetic nanoparticles(MNP)and red QDs using mesoporous silica nanoparticles(MSN)as a template.The magnetic driven-QRF-immunosensor was constructed based on green QDs probes-functionalized microplate.The results showed that the linear range of magnetic-fluorescence probes for the detection of CRP was 0.1–1000 ng/mL,with a sensitivity of up to 0.05 ng/mL.Compared to conventional QRF-immunosensor,the detection time was halved(within 30 min),and the sensitivity improved by approximately 5-fold.Recovery experiments and clinical sample analyses also demonstrated the good accuracy of this immunoassay.Therefore,the constructed novel magnetic driven-QRF-immunosensor shows great potential for clinical diagnosis and disease monitoring,and is expected to be applied in POC detection as a new generation of detection technology.展开更多
摘要A magnetic-fluorescent nanocomposite(CDs-MNPs)was synthesized via a facile one-pot microwave hy-drothermal method using polyethyleneimine(PEI)as a bridging agent.PEI connected magnetic nanoparticles(MNPs)and carbon dots(CDs),forming a fluffy clustered structure with nanoscale gaps,which effectively sup⁃pressed aggregation-induced photoluminescence quenching(AIQ).CDs-MNPs exhibited a saturation magnetization of 10.4 emu/g and stable solid-state green fluorescence.Benefiting from integrated magnetism,fluorescence,and strong adsorption to fingerprint residues,it achieved clear latent fingerprint(LFP)visualization on dark substrates.
基金the financial support from the research project of the Science and Technology Department of Henan Province(No.242102231045)Natural Science Foundation of Henan Province(No.232300420261)+1 种基金the National Natural Science Foundation of China(Nos.22405071,51972098)Natural Science Foundation of Liaoning Province(No.2022-BS-363).
摘要Efficient,sensitive,and convenient quantum dots(QDs)fluorescent immunosensors have a wide range of applications in the field of in vitro diagnostics(IVD).However,traditional single-signal readout methods are susceptible to interference,have limited sensitivity,and involve time-consuming detection processes.These have become key bottlenecks restricting their use in point-of-care(POC)detection.Herein,we developed a novel immunosensor based on QDs ratiometric fluorescence(QRF)and magnetic driven technology for sensitive,rapid,accurate detection of C-reactive protein(CRP).High-quality magnetic-fluorescent QDs nanobeads(QBs)were prepared by efficiently and orderly layered loading of magnetic nanoparticles(MNP)and red QDs using mesoporous silica nanoparticles(MSN)as a template.The magnetic driven-QRF-immunosensor was constructed based on green QDs probes-functionalized microplate.The results showed that the linear range of magnetic-fluorescence probes for the detection of CRP was 0.1–1000 ng/mL,with a sensitivity of up to 0.05 ng/mL.Compared to conventional QRF-immunosensor,the detection time was halved(within 30 min),and the sensitivity improved by approximately 5-fold.Recovery experiments and clinical sample analyses also demonstrated the good accuracy of this immunoassay.Therefore,the constructed novel magnetic driven-QRF-immunosensor shows great potential for clinical diagnosis and disease monitoring,and is expected to be applied in POC detection as a new generation of detection technology.