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The driving role of extracellular polymeric substances in the bioelectrical conversion of petroleum hydrocarbons by rhizosphere microbial fuel cells:bioelectricity production,substrate bioconversion,microbial function and their network correlation 认领 引用
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作者 Lanmei Zhao Yuru Wen +3 位作者 Lyu Can Long Meng Jian Liu Dong Zhao 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期1245-1259,共15页
Extracellular polymeric substances(EPS),are crucial components of biofilms that drive the bioelectrical conversion of petroleum hydrocarbons(PHCs),but their role has not been adequately addressed.This research explore... Extracellular polymeric substances(EPS),are crucial components of biofilms that drive the bioelectrical conversion of petroleum hydrocarbons(PHCs),but their role has not been adequately addressed.This research explores the driving role of EPS in bioelectrical PHC conversion by rhizosphere microbial fuel cells(MFCs).We found that current density,output voltage,coulombic efficiency,power density,current stabilization time,metabolite volatile fatty acid(VFA)production and PHC biodegradation ratio initially increased and then decreased with rising initial EPS level(0-128 mg·g-1),peaking at 64±1 mA·m−2,8.04±0.16 V,60.9±1.2%,129±3 mW·m−2,23±1 days,1.77±0.04 g·kg-1 and 66.7±1.5%,respectively.Fluorescence intensity of proteins having tyrosine-tryptophan demonstrated a continuous enhancement,consistent with increased biofilm thickness.Within an appropriate range of initial EPS levels(0-64 mg·g-1),bioelectricity generation and PHC bioconversion enhanced as the EPS content rose in mature biofilms.However,excessive EPS addition could increase biofilm thickness to 0.48 mm,which in turn reduced biofilm activity and overall system performance.The abundances of electrochemically active and PHC-degrading bacteria presented an initial increase followed by a subsequent decrease as the initial EPS level rose,highlighting that EPS at the optimal level enriched and activated these functional bacteria.The positive correlations between the relative abundances of these bacteria and various metrics of bioelectricity generation and PHC bioconversion underscored the critical role of EPS in shaping microbial community structure and enhancing electron transfer efficiency through biofilm formation and stabilization.These findings not only provide a critical theoretical foundation and novel ideas to promote the conversion of PHC into renewable bioenergy but also highlight the potential scalability and environmental benefits of this technology in the field of clean remediation of PHC-polluted soils and recovery of bioenergy.Integrating EPS-driven MFCs with other renewable energy technologies will offer promising opportunities to develop hybrid systems that generate clean energy while mitigating environmental pollution.Furthermore,this approach also has the potential as biosensors for the real-time detection of PHCs,thus contributing to broadening its application in environmental monitoring. 展开更多
关键词 Bioelectricity generation Bioelectrical conversion Microbial function Network correlation Extracellular polymeric substances
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Effects of Continuous Cropping of Polygonatum odoratum on Microbial Functional Diversity and Selected Environmental Factors in the Rhizosphere Soil 认领 引用
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作者 Manjiang YU Yihong HU +3 位作者 Fan XIE Xiongmei ZHU Chenzhong JIN Jing YE 《Agricultural Biotechnology》 2025年第5期12-18,21,共7页
[Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-cr... [Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-crop control(with Phaseolus vulgaris as the preceding crop),and blank control.The effects of continuous cropping on the functional diversity of soil microorganisms,soil enzyme activities,and soil nutrient coordination in the rhizosphere soil of P.odoratum during different growth stages were investigated.[Results](1)Continuous cropping increased the carbon source metabolic capacity,Shannon diversity index,and richness of rhizosphere soil microorganisms by 3.2%-14.7%,0.9%-3.5%,and 1.3%-12.5%,respectively,but the differences were not significant.(2)Principal component analysis indicated that during the middle stage of rhizome expansion,continuous cropping significantly altered the characteristics of microbial carbon metabolism,and the microbial communities utilizing carbohydrates,amino acids,polymers,carboxylic acids and amines as carbon sources exhibited vigorous metabolism.(3)Continuous cropping significantly reduced the activities of urease,polyphenol oxidase,and acid phosphatase in rhizosphere soil,with decreases of 24.4%-39.5%,3.2%-14.8%,and 7.9%-18.2%,respectively.The activities of catalase and invertase sometimes exceeded and sometimes fell below those of the first crop,showing no consistent pattern.(4)Under continuous cropping conditions,nutrient imbalance occurred in the rhizosphere soil,characterized by nitrogen deficiency and phosphorus surplus.(5)Grey correlation analysis indicated that available phosphorus content,alkali-hydrolyzable nitrogen content and polyphenol oxidase activity in rhizosphere soil were the main factors influencing microbial functional diversity.[Conclusions]This study provides a theoretical basis for understanding the formation mechanism of continuous cropping obstacles in P.odoratum. 展开更多
关键词 Polygonatum odoratum Continuous cropping Rhizosphere soil Microbial function
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Degradation of chlorpyrifos in laboratory soil and its impact on soil microbial functional diversity 认领 引用 被引量：26
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作者 FANG Hua YU Yunlong +3 位作者 CHU Xiaoqiang WANG Xiuguo YANG Xiaoe YU Jingquan 《Journal of Environmental Sciences》 SCIE EI CAS 2009年第3期380-386,共7页
Degradation of chlorpyrifos at different concentrations in soil and its impact on soil microbial functional diversity were investigated under laboratory condition. The degradation half-live of chlorpyrifos at levels o... Degradation of chlorpyrifos at different concentrations in soil and its impact on soil microbial functional diversity were investigated under laboratory condition. The degradation half-live of chlorpyrifos at levels of 4, 8, and 12 mg/kg in soil were calculated to be 14.3, 16.7, and 18.0 d, respectively. The Biolog study showed that the average well color development （AWCD） in soils was significantly （P 〈 0.05） inhibited by chlorpyrifos within the first two weeks and thereafter recovered to a similar level as the control. A similar variation in the diversity indices （Simpson index lID and McIntosh index U） was observed, but no significant difference among the values of the Shannon-Wiener index H＇ was found in chlorpyrifos-treated soils. With an increasing chlorpyrifos concentration, the half-life of chlorpyrifos was significantly （P ≤ 0.05） extended and its inhibitory effect on soil microorganisms was aggravated. It is concluded that chlorpyrifos residues in soil had a temporary or short-term inhibitory effect on soil microbial functional diversity. 展开更多
关键词 Biolog chlorpyrifos community-level physiological profile microbial functional diversity
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Tolerance of Grasses to Heavy Metals and Microbial Functional Diversity in Soils Contaminated with Copper Mine Tailings 认领 引用 被引量：21
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作者 TENG Ying LUO Yong-Ming +3 位作者 HUANG Chang-Yong LONG Jian LI Zhen-Gao P. CHRISTIE 《Pedosphere》 SCIE CAS 2008年第3期363-370,共8页
Copper （Cu） mine tailings, because of their high content of heavy metals, are usually hostile to plant colonization. A pot experiment was conducted to determine the tolerance of four forage grasses to heavy metals i... Copper （Cu） mine tailings, because of their high content of heavy metals, are usually hostile to plant colonization. A pot experiment was conducted to determine the tolerance of four forage grasses to heavy metals in Cu mine tailings and to examine the variation in the microbial functional diversity of soils from the tailing sites in southern China. All the four grass species survived on Cu mine tailings and Cu mine tailing-soil mixture. However, on pure mine tailings, the growth was minimal, whereas the growth was maximum for the control without mine tailings. The tolerance of grasses to heavy metals followed the sequence： Paspalum notatum 〉 Festuea arundinaeea 〉 Lolium perenne 〉 Cynodon daetylon. The planting of forage grasses enhanced the soil microbial biomass. The Biolog data indicated that the soil microbial metabolic profile values （average well color development, community richness, and Shannon index） of the four forage grasses also followed the sequence： P. notatum 〉 F. arundinaeea 〉 L. perenne 〉 C. daetylon. Thus, P. notatum, under the experimental conditions of this study, may be considered as the preferred plant species for revegetation of Cu mine tailing areas. 展开更多
关键词 copper mine tailings forage grass heavy metal pollution microbial functional diversity
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Microbial inoculants modify the functions of soil microbes to optimize plant growth at abandoned mine sites 认领 引用 被引量：2
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作者 Zhaohui Jia Chong Li +4 位作者 Shuifeng Zhang Yingzhou Tang Shilin Ma Xin Liu Jinchi Zhang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2025年第8期678-690,共13页
Mining activities have caused significant land degradation globally,emphasizing the need for effective restoration.Microbial inoculants offer a promising solution for sustainable remediation by enhancing soil nutrient... Mining activities have caused significant land degradation globally,emphasizing the need for effective restoration.Microbial inoculants offer a promising solution for sustainable remediation by enhancing soil nutrients,enzyme activities,and microbial communities to support plant growth.However,the mechanisms by which inoculants influence soil microbes and their relationship with plant growth require further investigation.Metagenomic sequencing was employed for this study,based on a one-year greenhouse experiment,to elucidate the effects of Bacillus thuringiensis NL-11 on the microbial functions of abandoned mine soils.Our findings revealed that the application of microbial inoculants significantly enhanced the soil total carbon(TC),total sulfur(TS),organic carbon(SOC),available phosphorus(AP),ammonium(NH4+),urease,arylsulfatase,phosphatase,β-1,4-glucosidase(BG),β-1,4-N-acetylglucosaminidase(NAG).Moreover,this led to substantial improvements in plant height,as well as aboveground and belowground biomass.Microbial inoculants impacted functional gene structures without altering diversity.The normalized abundance of genes related to the degradation of carbon and nitrogen,methane metabolism,and nitrogen fixation were observed to increase,as well as the functional genes related to phosphorus cycling.Significant correlations were found between nutrient cycling gene abundance and plant biomass.Partial Least Squares Path Model analysis showed that microbial inoculants not only directly influenced plant biomass but also indirectly affected the plant biomass through C cycle modifications.This study highlights the role of microbial inoculants in promoting plant growth and soil restoration by improving soil properties and enhancing normalized abundance of nutrient cycling gene,making them essential for the recovery of abandoned mine sites. 展开更多
关键词 Mine restoration Microbial inoculant Metagenomics Microbial function Plant growth
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Arbuscular mycorrhizal symbiosis enhances microbial contribution to mineral-associated organic carbon persistence in soil:Insights from soil microbial community and microbial necromass carbon 认领 引用
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作者 Yin LIU Jin QIAN +4 位作者 Yueming ZHU Jing HU Bianhe LU Yuxuan HE Junwei SHEN 《Pedosphere》 SCIE CAS CSCD 2026年第2期540-553,共14页
The formation of mineral-associated organic carbon(MAOC),a critical soil fraction related to soil organic carbon(SOC)sequestration,is significantly influenced by microbial processes.Yet,how arbuscular mycorrhizal(AM)s... The formation of mineral-associated organic carbon(MAOC),a critical soil fraction related to soil organic carbon(SOC)sequestration,is significantly influenced by microbial processes.Yet,how arbuscular mycorrhizal(AM)symbiosis impacts MAOC accumulation in rhizosphere soils through its effects on microbial contribution remains poorly understood.Here,a pot experiment was conducted in a climate chamber using Lythrum salicaria L.as the host plant and Archaeospora trappei as the AM fungi inoculum to assess the microbial community in the samples collected from bulk soil(BS)and rhizosphere soil(RS)under the AM fungi inoculation(+AM)and non-inoculation(-AM)treatments using 16S r RNA sequencing.The results showed that AM symbiosis influenced soil microbial community composition and enhanced soil microbial community functions related to carbon(C)degradation.The number of live and dead cells(all cells in the soil)was the highest in RS under the+AM treatment(RS+AM)based on confocal laser scanning microscopy images.Meanwhile,AM symbiosis increased the proportions of apoptotic cells(19.02%)and necrotic cells(12.12%)in RS.Using soil amino sugars as microbial biomarkers,the concentrations of bacterial necromass C(188.01 g kg-1OC)and fungal necromass C(392.19 g kg-1OC)were the highest in the MAOC fraction in RS+AM.Additionally,the MAOC content(17.20 g kg-1soil)and proportion(42.06%)were the highest in RS+AM.This study illustrated two primary mechanisms by which AM symbiosis enhanced MAOC accumulation:1)altering soil microbial community composition and functions related to C degradation and 2)promoting the input of microbial necromass C,especially fungal necromass C.This study broadens our horizons to understand the mechanisms of microbial contribution to MAOC accumulation stimulated by AM symbiosis in rhizosphere soils and provides management practices for the application of AM fungi to SOC sequestration. 展开更多
关键词 arbuscular mycorrhizal fungi carbon sequestration fungal necromass carbon microbial community function soil carbon degradation soil organic carbon
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Increasing tree phylogenetic diversity stimulates microbial functional potential in a subtropical forest 认领 引用 被引量：3
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作者 Yuantian Fang Xunyang He +1 位作者 Qingquan Xie Dejun Li 《Journal of Plant Ecology》 SCIE CSCD 2024年第6期307-319,共13页
Soil microbial functions are closely related to ecosystem productivity,carbon sequestration and their responses to global change.Tree phylogenetic diversity(TPD)has been found to impact microbial community composition... Soil microbial functions are closely related to ecosystem productivity,carbon sequestration and their responses to global change.Tree phylogenetic diversity(TPD)has been found to impact microbial community composition,diversity and functions,but how it modulates the linkage between microbial community facets and functions remains unclear.Here,45 plots covering a natural gradient of TPD were selected in a subtropical forest of southwest China to explore how increasing TPD impacts soil microbial community facets and microbial functional potential.The microbial functional potential was evaluated based on the abundances of carbon,nitrogen and phosphorus cycling-related functional genes.Soil fungal alpha diversity increased significantly,but bacterial alpha diversity did not change as TPD increased.Both soil microbial network complexity and stability improved significantly with increasing TPD.Ultimately,increasing TPD promoted soil microbial functional potential by stimulating soil carbon and nitrogen availability,microbial keystone diversity and network stability collectively.These findings emphasize the critical roles of keystone taxa and network stability as microbial factors in stimulating soil microbial function in response to increasing TPD.Therefore,it is strongly recommended to increase TPD so as to stimulate soil microbial functions and other ecosystem functions when implementing afforestation or ecological restoration projects. 展开更多
关键词 biodiversity microbial functional potential fungal-bacterial interactions tree phylogenetic diversity subtropical forest
Microbial community structure and functional metabolic diversity are associated with organic carbon availability in an agricultural soil 认领 引用 被引量：9
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作者 LI Juan LI Yan-ting +3 位作者 YANG Xiang-dong ZHANG Jian-jun LIN Zhi-an ZHAO Bing-qiang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第12期2500-2511,共12页
Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental ... Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included： a non-amended control（CK）, a commonly used application rate of inorganic fertilizer treatment（NPK）; a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment（NPKM）, and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment（NPKS）. Denaturing gradient gel electrophoresis（DGGE） of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term（NPKM, NPKS） significantly promoted soil bacterial structure than the application of inorganic fertilizer only（NPK）, and NPKM treatment was the most important driver for increases in the soil microbial community richness（S） and structural diversity（H）. Overall utilization of carbon sources by soil microbial communities（average well color development, AWCD） and microbial substrate utilization diversity and evenness indices（H＇ and E） indicated that long-term inorganic fertilizer with organic amendments incorporated（NPKM, NPKS） could significantly stimulate soil microbial metabolic activity and functional diversity relative to CK, while no differences of them were found between NPKS and NPK treatments. Principal component analysis（PCA） based on carbon source utilization profiles also showed significant separation of soil microbial community under long-term fertilization regimes and NPKM treatment was significantly separated from the other three treatments primarily according to the higher microbial utilization of carbohydrates, carboxylic acids, polymers, phenolic compounds, and amino acid, while higher utilization of amines/amides differed soil microbial community in NPKS treatment from those in the other three treatments. Redundancy analysis（RDA） indicated that soil organic carbon（SOC） availability, especially soil microbial biomass carbon（Cmic） and Cmic/SOC ratio are the key factors of soil environmental characteristics contributing to the increase of both soil microbial community structure and functional metabolic diversity in the long-term fertilization trial. Our results showed that long-term inorganic fertilizer and swine manure application could significantly improve soil bacterial community structure and soil microbial metabolic activity through the increases in SOC availability, which could provide insights into the sustainable management of China＇s soil resource. 展开更多
关键词 long-term fertilization regimes organic amendment soil microbial community structure microbial functional metabolic activity carbon substrate utilization
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Metagenomic insights into seasonal variations in the soil microbial community and function in a Larix gmelinii forest of Mohe,China 认领 引用 被引量：1
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作者 Tong Bao Shilin Deng +2 位作者 Kaiyue Yu Weiyi Li Airong Dong 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第1期371-383,共13页
The eff ect of seasons on the soil microbiome in a Larix gmelinii forest of Mohe,China,where winter temperatures are generally below−40°C,was evaluated with metagenomics analysis.Taxonomic profi ling using sequen... The eff ect of seasons on the soil microbiome in a Larix gmelinii forest of Mohe,China,where winter temperatures are generally below−40°C,was evaluated with metagenomics analysis.Taxonomic profi ling using sequencing information revealed that Proteobacteria,Actinobacteria,Acidobacteria and Verrucomicrobia were the dominant phyla in spring,summer,and fall,as were Bradyrhizobium,Chthoniobacter,Streptomyces,Acid Candidatus Koribacter at the genus level.Some species that were abundant in spring and fall greatly diminished in abundance in summer.Clusters of orthologous groups(COG)of proteins,carbohydrate-active enzymes(CAZy),Kyoto Encyclopedia of Genes and Genomes(KEGG)and NCBI databases were used to elucidate the function of diverse proteins and metabolites of the microbial community of L.gmelinii forest.COG analysis showed that fewer genes were detected in spring than in fall and summer,indicating that many soil microbes in the L.gmelinii forest were not tolerant to cold.Based on KEGG analysis,some pathways in the soil microbes were activated in spring and autumn and deactivated in summer.CAZy analysis revealed that most CAZy were more active in summer than in spring or autumn and were severely inhibited in the spring.Many functional pathways,proteins,and CAZy involved in the community changes were concerned with cold or heat resistance.Therefore,the soil in the L.gmelinii forest can be a valuable resource for further research on heat and cold tolerance of soil microbes. 展开更多
关键词 Soil microbial community Microbial function Metagenomic Seasonal variation
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Effect of Long-Term Application of Chemical Fertilizers on Microbial Biomass and Functional Diversity of a Black Soil 认领 引用 被引量：25
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作者 KONG Wei-Dong ZHU Yong-Guan +3 位作者 FU Bo-Jie HAN Xiao-Zeng ZHANG Lei HE Ji-Zheng 《Pedosphere》 SCIE CAS 2008年第6期801-808,共8页
An experiment with seven N, P, K-fertilizer treatments, i.e., control (no fertilizer), NP, NK, PK, NPK, NP2K, and NPK2 where P2 and K2 indicate double amounts of P and K fertilizers respectively, was conducted to exam... An experiment with seven N, P, K-fertilizer treatments, i.e., control (no fertilizer), NP, NK, PK, NPK, NP2K, and NPK2 where P2 and K2 indicate double amounts of P and K fertilizers respectively, was conducted to examine the effect of long-term continuous application of chemical fertilizers on microbial biomass and functional diversity of a black soil (Udoll in the USDA Soil Taxonomy) in Northeast China. The soil microbial biomass C ranged between 94 and 145 mg kg-1, with the NK treatment showing a lower biomass; the functional diversity of soil microbial community ranged from 4.13 to 4.25, with an increasing tendency from control to double-fertilizer treatments, and to triple-fertilizer treatments. The soil microbial biomass, and the microbial functional diversity and evenness did not show any significant differences among the different fertilizer treatments including control, suggesting that the long-term application of chemical fertilization would not result in significant changes in the microbial characteristics of the black soil. 展开更多
关键词 Biolog black soil chemical fertilizer microbial biomass microbial functional diversity
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Structure and functional heterogeneity of soil microbial communities in different farmland types on the Loess Plateau 认领 引用 被引量：2
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作者 XU Chen SU Cuicui +3 位作者 CHEN Yiping MA Jifu WU Junhua JIANG Yao 《地球环境学报》 CSCD 2024年第4期653-664,共12页
Background,aim,and scope Soil microbes are important drivers of nutrient transformation and energy f low.Reclaiming forest land for agricultural use may have profound effects on soil properties and microbial communiti... Background,aim,and scope Soil microbes are important drivers of nutrient transformation and energy f low.Reclaiming forest land for agricultural use may have profound effects on soil properties and microbial communities.However,the response of soil microbial communities to soil reclamation in the dryland agroecosystem is less understood.Therefore,it is necessary to investigate the changes of soil microbial communities driven by land use conversion to promote nutrient cycling in reclaimed farmland.Materials and methods Based on the metagenomic technique,we evaluated the microbial composition and function of the newly created farmland(NF)after reclamation with two types of traditional farmland(slope farmland(SF),checkdam farmland(CF))on the Loess Plateau,and explored the response of nutrient cycling function to dominant genera and soil properties.Results The results showed that Proteobacteria,Actinobacteria,and Acidobacteria were prevalent in the three types of farmlands.Compared with SF and CF,NF increased the relative abundance of Actinobacteria and Nocardioides,as well as genes related to amino acid metabolism and carbohydrate metabolism.The relative abundance of functional genes related to carbon and nitrogen cycling in the NF was higher than that in the traditional farmland(SF and CF).The relative abundance of nutrient cycling functional genes was positively correlated with dominant genera in the three types of farmlands.Except for pH,soil physicochemical factors were negatively correlated with genes related to amino acid metabolism and carbon cycle.Discussion Previous studies have shown that the nutrient conditions of the soil may intensify the competition between the eutrophic and oligotrophic microbial populations.After long-term cultivation and fertilization,the soil properties of traditional farmland were significantly different from those of NF,leading to the differentiation of dominant microbial groups.Microbes usually have functional redundancy to cope with changing environments.Soil microbes in traditional farmland may contain more genes related to replication and repair,cell growth and death,and environmental adaptation in response to disturbances caused by agricultural practices.On the contrary,the NF was less disturbed by agricultural activities,and the soil properties were more similar to forest land,so the carbon and nitrogen cycle function genes were more abundant.The nutrient cycling function was affected by the abundance of microbial dominant groups and soil properties,which may be related to the availability of soil nutrients and agricultural disturbance in different farmlands.Aspects of soil microbial-driven nutrient cycling in agriculture could be regulated in sustainable method.Conclusions The change from forest land to farmland kept more carbon and nitrogen cycling function in the newly created farmland,while long-term agricultural activities have drastically changed the functional structure of traditional farmland,resulting in the nutrient cycling function more concentrated to meet the needs of crop growth.Recommendations and perspectives Hence,it is necessary to apply sustainable agricultural method to regulate microbial-driven nutrient cycling.The insights are meaningful for sustainable agricultural development and land management in arid areas. 展开更多
关键词 metagenomics microbial function farmland nutrient cycling Loess Plateau
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Effects of turning frequency on fermentation efficiency and microbial community metabolic function of sheep manure composting on the Qinghai-Tibet Plateau 认领 引用 被引量：6
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作者 Rui Cai Sasa Zuo +2 位作者 Xiaohui Cao Xin Jiang Chuncheng Xu 《Bioresources and Bioprocessing》 SCIE EI 2023年第1期649-663,共15页
This study explored the effects of turning frequency on fermentation efficiency and microbial metabolic function of sheep manure composting on the Qinghai-Tibet Plateau(QTP).Five treatments with different turning freq... This study explored the effects of turning frequency on fermentation efficiency and microbial metabolic function of sheep manure composting on the Qinghai-Tibet Plateau(QTP).Five treatments with different turning frequencies were set up in this study:turning every 1 day(T1),2 days(T2),4 days(T3),6 days(T4),and 8 days(T5).Results showed that the high temperature period for T1 and T5 lasted only 4 days,while that for T2-T4 lasted more than 8 days.The germination index of T1 and T5 was lower than 80%,while that of T2-T4 was 100.6%,97.8%,and 88.6%,respectively.This study further predicted the microbial metabolic function of T2-T4 using the bioinformatics tool PICRUSt2(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States)and determining the activities of various functional enzymes.The results showed that carbohydrate metabolism,protein metabolism,and nucleotide metabolism were the main metabolic pathways of microorganisms,and that T2 increased the abundance of functional genes of these metabolic pathways.The activities of protease,cellulase,and peroxidase in T2 and T3 were higher than those in T4,and the effect of T2 was more significant.In conclusion,turning once every 2 days can improve the quality of sheep manure compost on the QTP. 展开更多
关键词 Sheep manure composting Qinghai-Tibet Plateau Turning frequency Fermentation efficiency Microbial community metabolic function
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Microbial Community Composition and Function in Sediments from the Pearl River Mouth Basin 认领 引用 被引量：4
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作者 CHEN Ye MI Tiezhu +2 位作者 LIU Yueteng LI Siqi ZHEN Yu 《Journal of Ocean University of China》 SCIE CAS CSCD 2020年第4期941-953,共13页
This study was conducted to characterize the diversity and function of microbial communities in marine sediments of the Pearl River Mouth Basin(PRMB)in the South China Sea.The results showed that the bacterial and arc... This study was conducted to characterize the diversity and function of microbial communities in marine sediments of the Pearl River Mouth Basin(PRMB)in the South China Sea.The results showed that the bacterial and archaeal communities varied greatly with depth.Proteobacteria in bacterial communities and Nitrososphaeria and Woesearchaeota in archaeal communities were dominant in the shallow sediments(1-40 cm),while Chloroflexi in bacterial communities and Bathyarchaeia in archaeal communities were dominant in the deep sediments(50-200 cm).Regarding ecological functions based on the metatranscriptomic data,genes involved in various pathways of nitrogen metabolism and sulfur metabolism were observed in the tested sediment samples.Metagenomic analysis revealed that Proteobacteria contribute the most to nearly all genes involved in nitrogen and sulfur metabolism.Moreover,Thaumarchaeota contribute the most to certain genes involved in nitrification,denitrification and assimilatory sulfate reduction pathways.The most abundant bacterial genus,Candidatus Scalindua,is crucial for nitrification,dissimilatory nitrate reduction,denitrification and assimilatory sulfate reduction pathways. 展开更多
关键词 microbial community and function high-throughput sequencing Pearl River Mouth Basin(PRMB)
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Changes in Transformation of Soil Organic C and Functional Diversity of Soil Microbial Community Under Different Land Uses 认领 引用 被引量：24
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作者 LI Zhong-pei WU Xiao-chen CHEN Bi-yun 《Agricultural Sciences in China》 2007年第10期1235-1245,共11页
Changes in soil biological and biochemical properties under different land uses in the subtropical region of China were investigated in order to develop rational cultivation and fertilization management. A small water... Changes in soil biological and biochemical properties under different land uses in the subtropical region of China were investigated in order to develop rational cultivation and fertilization management. A small watershed of subtropical region of China was selected for this study. Land uses covered paddy fields, vegetable farming, fruit trees, upland crops, bamboo stands, and forestry. Soil biological and biochemical properties included soil organic C and nutrient contents, mineralization of soil organic C, and soil microbial biomass and community functional diversity. Soil organic C and total N contents, microbial biomass C and N, and respiration intensity under different land uses were changed in the following order： paddy fields （and vegetable farming） 〉 bamboo stands 〉 fruit trccs （and upland）. The top surface （0-15 cm） paddy fields （and vegetable farming） were 76.4 and 80.8% higher in soil organic C and total N contents than fruit trees （and upland） soils, respectively. Subsurface paddy soils （15-30 cm） were 59.8 and 67.3% higher in organic C and total N than upland soils, respectively. Soil microbial C, N and respiration intensity in paddy soils （0-15 cm） were 6.36, 3.63 and 3.20 times those in fruit tree （and upland） soils respectively. Soil microbial metabolic quotient was in the order： fruit trees （and upland） 〉 forestry 〉 paddy fields. Metabolic quotient in paddy soils was only 47.7% of that in fruit tree （and upland） soils. Rates of soil organic C mineralization during incubation changed in the order： paddy fields 〉 bamboo stands 〉 fruit trees （and upland） and soil bacteria population： paddy fields 〉 fruit trees （and upland） 〉 forestry. No significant difference was found for fungi and actinomycetes populations. BIOLOG analysis indicated a changing order of paddy fields 〉 fruit trees （and upland） 〉 forestry in values of the average well cell development （AWCD） and functional diversity indexes of microbial community. Results also showed that the conversion from paddy fields to vegetable farming for 5 years resulted in a dramatic increase in soil available phosphorus content while insignificant changes in soil organic C and total N content due to a large inputs of phosphate fertilizers. This conversion caused 53, 41.5, and 41.3% decreases in soil microbial biomass C, N, and respiration intensity, respectively, while 23.6% increase in metabolic quotient and a decrease in soil organic C mineralization rate. Moreover, soil bacteria and actinomycetes populations were increased slightly, while fungi population increased dramatically. Functional diversity indexes of soil microbial community decreased significantly. It was concluded that land uses in the subtropical region of China strongly affected soil biological and biochemical properties. Soil organic C and nutrient contents, mineralization of organic C and functional diversity of microbial community in paddy fields were higher than those in upland and forestry. Overuse of chemical fertilizers in paddy fields with high fertility might degrade soil biological properties and biochemical function, resulting in deterioration of soil biological quality. 展开更多
关键词 land use patterns transformation of soil organic carbon functional diversity of soil microbial community
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Application Effect of Functional Microbial Products in the Cultivation of Green Ecological Strawberry 认领 引用
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作者 Houjun HE Shuwei YAO +2 位作者 Defu LI Muxiang JI Yushan QIAO 《Asian Agricultural Research》 2023年第2期46-47,54,共2页
In order to promote the transformation and high-quality development of strawberry industry and speed up the application of functional microbial products in strawberry,the application technology of functional microbial... In order to promote the transformation and high-quality development of strawberry industry and speed up the application of functional microbial products in strawberry,the application technology of functional microbial products in strawberry planting is studied and summarized.It mainly includes:the periods and methods of continuous cropping strawberry soil reduction and disinfection at high temperature,before and after strawberry planting,before plastic film mulching and whole process of plant spraying.Through multi-point test and demonstration,the purpose of advancing the season of strawberries,improving the quality,increasing production and increasing efficiency is achieved,and there is no risk of agricultural residue pollution,which meets the needs of people's consumption upgrading.Therefore,the application of functional microbial products in green ecological planting is very necessary,and it is worth further speeding up the demonstration and promotion. 展开更多
关键词 Functional microbial products Strawberry cultivation Application effect
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Effect of Intensive Inorganic Fertilizer Application on Microbial Properties in a Paddy Soil of Subtropical China 认领 引用 被引量：5
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作者 LIU Ming Klemens Ekschmitt +4 位作者 ZHANG Bin Stephanie IJHolzhauer LI Zhong-pei ZHANG Tao-lin Sabine Rauch 《Agricultural Sciences in China》 CSCD 2011年第11期1758-1764,共7页
A field experiment with rice-rice rotation was conducted since 2002 in southeast China for investigating the response of soil microbial properties to intensive nitrogen fertilizer application.The tested soil was a sub... A field experiment with rice-rice rotation was conducted since 2002 in southeast China for investigating the response of soil microbial properties to intensive nitrogen fertilizer application.The tested soil was a subtropical paddy soil derived from Quaternary red clay.Differences between treatments existed in different application rates of urea when the experiment was designed.Urea was applied in five rates,i.e.,0,0.5,1,1.5,and 2 U,equivalent to 0,0.5,1,1.5,and 2 times the local average amount of urea application(900 kg urea ha-~yr-~,equivalent to 414 kg N ha-1 yr-~).In 2007,soil total nitrogen,available nitrogen,and soil organic carbon contents were increased by 10.2-27.9,8.0-16.0,and 10.2-30.6%,respectively,in treatments with urea application rates of 0.5 to 2 U compared to control(0 U).Microbial biomass carbon and nitrogen were also increased by 3.1-30.8 and 1.3-13.9%,respectively,in treatments with urea application.Basal respiration in treatments with urea input were 9.4-29.1%higher than that in control.However,changes of bacterial functional diversity had different trends.Urea fertilization enhanced bacterial functional diversity until treatment of 1 U,but re-decreased it from treatment of 1.5 U.Principal components analysis indicated that there were intimate relationships among soil organic matter,nitrogen nutrient,microbial biomass,and respiration.Nevertheless,microbial diversity was related to soil moisture contents after urea application.We conclude here that the application of N fertilizer improved soil microbial biomass and respiratory activity.But,microbial diversity was reduced when excessive urea was applied in the tested paddy soil. 展开更多
关键词 paddy soil intensive N application microbial properties microbial functional diversity red soil region
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Soil physicochemical and microbial drivers of temperature sensitivity of soil organic matter decomposition under boreal forests 认领 引用 被引量：2
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作者 Beata KLIMEK Marcin CHODAK +2 位作者 Matgorzata JAZWA Hamed AZARBAD Maria NIKLINSKA 《Pedosphere》 SCIE CAS CSCD 2020年第4期528-534,共7页
Soil organic matter(SOM)in boreal forests is an important carbon sink.The aim of this study was to assess and to detect factors controlling the temperature sensitivity of SOM decomposition.Soils were collected from Sc... Soil organic matter(SOM)in boreal forests is an important carbon sink.The aim of this study was to assess and to detect factors controlling the temperature sensitivity of SOM decomposition.Soils were collected from Scots pine,Norway spruce,silver birch,and mixed forests(O horizon)in northern Finland,and their basal respiration rates at five different temperatures(from 4 to 28℃)were measured.The Q10 values,showing the respiration rate changes with a 10℃ increase,were calculated using a Gaussian function and were based on temperature-dependent changes.Several soil physicochemical parameters were measured,and the functional diversity of the soil microbial communities was assessed using the MicroResp?method.The temperature sensitivity of SOM decomposition differed under the studied forest stands.Pine forests had the highest temperature sensitivity for SOM decomposition at the low temperature range(0–12℃).Within this temperature range,the Q10 values were positively correlated with the microbial functional diversity index(H’mic)and the soil C-to-P ratio.This suggested that the metabolic abilities of the soil microbial communities and the soil nutrient content were important controls of temperature sensitivity in taiga soils. 展开更多
关键词 CoO2 evolution global warming microbial functional diversity MicroResp^TM Q10 metabolic coefficient
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Lowered water table causes species substitution while nitrogen amendment causes species loss in alpine wetland microbial communities 认领 引用 被引量：1
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作者 Yuntao LI Jin-Sheng HE +3 位作者 Hao WANG Jizhong ZHOU Yunfeng YANG Haiyan CHU 《Pedosphere》 SCIE CAS CSCD 2021年第6期912-922,共11页
Alpine wetlands are hotspots of carbon(C)storage and methane emission,and they could be key contributors to global warming.In recent years,rapid warming has lowered the water table in alpine wetlands on the Tibetan Pl... Alpine wetlands are hotspots of carbon(C)storage and methane emission,and they could be key contributors to global warming.In recent years,rapid warming has lowered the water table in alpine wetlands on the Tibetan Plateau,concurrent with intensified nitrogen(N)deposition via anthropogenic activities.We carried out a field experiment to investigate the ecological impacts of these two factors on soil bacterial and functional communities,which are essential drivers of greenhouse gas emissions.Nitrogen amendment alone decreased the phylogenetic alpha-diversity of bacterial communities which could be offset by lowered water table.In contrast,microbial functional alpha-diversity,revealed by a high-throughput microarray,remained unchanged.Both bacterial and functional beta-diversity responded to lowered water table,but only bacterial community responded to N amendment.The alpha-Proteobacteria,beta-Proteobacteria,and Bacteroidetes were the major responsive bacterial lineages,and C degradation,methanogenesis,alkaline shock,and phosphorus oxidation were the major responsive functional processes.Partitioning analysis revealed that N amendment changed bacterial community structure mainly via species loss processes but did not affect bacterial functional communities,with soil pH and ammonium as the key factors influencing changes in bacterial community structure.Conversely,lowered water table altered bacterial and functional communities through species substitution processes linked to soil pH and soil moisture.According to our results,the response mechanisms of microbial communities to lowered water table and N amendment are fundamentally different in alpine wetlands. 展开更多
关键词 alpha-diversity bacterial community beta-diversity microbial functional community N amendment water table lowering
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Changes in bacterial community and abundance of functional genes in paddy soil with cry1Ab transgenic rice 认领 引用 被引量：1
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作者 SONG Ya-na CHEN Zai-jie +2 位作者 WU Ming-ji LI Gang WANG Feng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第6期1674-1686,共13页
A field experiment involving cry1Ab transgenic rice(GM) and its parental non-cry1Ab rice(M) has been on-going since 2014. The diversity of the bacterial communities and the abundance of the microbial functional genes ... A field experiment involving cry1Ab transgenic rice(GM) and its parental non-cry1Ab rice(M) has been on-going since 2014. The diversity of the bacterial communities and the abundance of the microbial functional genes which drive the conversion of nitrogen in paddy soil were analyzed during the growth period of rice in the fifth year of the experiment, using 16 S rRNAbased Illumina Mi Seq and real-time PCR on the amoA, nirS and nirK genes. The results showed no differences in the alpha diversity indexes of the bacterial communities, including Chao1, Shannon and Simpson, between the fields cultivated with line GM and cultivar M at any of the growth stages of rice. However, the bacterial communities in the paddy soil with line GM were separated from those of paddy soil with cultivar M at each of the growth stages of rice, based on the unweighted Uni Frac NMDS or PCoA. In addition, the analyses of ADONIS and ANOSIM, based on the unweighted Uni Frac distance, indicated that the above separations between line GM and cultivar M were statistically significant(P<0.05) during the growth season of rice. The increases in the relative abundances of Acidobacteria or Bacteroidetes, in the paddy soils with line GM or cultivar M, respectively, led to the differences in the bacterial communities between them. At the same time, functional gene prediction based on Illumina Mi Seq data suggested that the abundance of many functional genes increased in the paddy soil with line GM at the maturity stage of rice, such as genes related to the metabolism of starch, amino acids and nitrogen. Otherwise, the copies of bacterial amo A gene, archaeal amo A gene and denitrifying bacterial nir K gene significantly increased(P<0.05 or 0.01) in the paddy soil with line GM. In summary, the release of cry1Ab transgenic rice had effects on either the composition of bacterial communities or the abundance of microbial functional genes in the paddy soil. 展开更多
关键词 cry1Ab transgenic rice bacterial community microbial functional gene Illumina MiSeq Platform real-time PCR
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Investigation of kinetics in bioaugmentation of crude oil via high-throughput sequencing: Enzymatic activities, bacterial community composition and functions 认领 引用 被引量：3
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作者 Yong-Rui Pi Mu-Tai Bao 《Petroleum Science》 SCIE CAS CSCD 2022年第4期1905-1914,共10页
The enzymes and the characteristics of the community of the petroleum-degrading bacteria play a crucial role in the crude oil biodegradation. The prediction of kinetics of the key groups of hydrocarbons in crude oil w... The enzymes and the characteristics of the community of the petroleum-degrading bacteria play a crucial role in the crude oil biodegradation. The prediction of kinetics of the key groups of hydrocarbons in crude oil was important to evaluate the bioremediation speed and constant. Most of the n-alkanes(C9-C29) were degraded in 25 days, and the average degradation rates of C18~C27 higher than 100 μg g-1 d-1.The hopanes, such as H30, had a biodegradation rate more than 10 μg g-1 d-1. The related enzymes activities changed along with the crude oil biodegradation, especially dehydrogenase. The 16 S rRNA gene amplicon sequencing revealed that Proteobacteria, Firmcutes, Bacteroidetes, Actinobacteria, Acidobacteria were the main petroleum hydrocarbon degraders during the crude oil biodegradation, and the top two highest relative abundance of the genera were Alcaligenes and Acinetobacter. Acinetobacter presented positive correlation to biodegradation of n-alkanes and PAHs. Based on COG analysis, the largest group involved in the general function was amino acid transport and metabolism. The functional categories of bacterial communities were mainly focused on the carbohydrate and amino acid metabolism, xenobiotics biodegradation and metabolism, membrane transport, and so on. Overall, these findings highlight the potential guideline for more adequate monitoring of microbial degradation of crude oil. 展开更多
关键词 Crude oil Bioaugmentation Kinetics Enzyme activities Microbial functions
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