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Six-fold swelling expansion microscopy assisted cell painting for high-content phenotypic screening 认领 引用
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作者 Junfang XIE Yipeng DONG +3 位作者 Jin WANG Zhichao DENG Jianguo TIAN Qing YE 《Optoelectronics Letters》 EI 2026年第2期111-117,共7页
Imaging-based phenotypic screening uses cellular phenotypes to describe the drug performance, which generally focuses on single cellular feature, lacking of a comprehensive characterization. Here, we propose a high co... Imaging-based phenotypic screening uses cellular phenotypes to describe the drug performance, which generally focuses on single cellular feature, lacking of a comprehensive characterization. Here, we propose a high content phenotypic screening method based on expansion microscopy(ExM) assisted cell painting, which enables multi-channel imaging with approximately 50 nm resolution. As a demonstration, we applied this method to a phenotypic screening involving five drugs. The morphological attributes of three subcellular structures were summarized to consist a “fingerprint” describing the drug effect. The proposed method can provide comprehensive and detailed clues for drug evaluation, enriching the content of phenotypic screening. 展开更多
关键词 six fold swelling expansion microscopy cellular feature five drugs expansion microscopy exm phenotypic screening cell painting cellular phenotypes describe drug performance
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Correlating corneal and meibomian gland morphology with diabetes duration in T2DM via confocal microscopy 认领 引用
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作者 Ke-Jun Li Yin Zhang +4 位作者 Yi-Ze Han Zhi-Hua Zhao Fang Fan Yi-Ming Chen Jian-Min Wang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2026年第6期1149-1157,共9页
AIM:To investigate morphological changes in the cornea and meibomian glands using in vivo confocal microscopy(IVCM)in patients with type 2 diabetes(T2DM)and to analyze the correlation of these changes with disease dur... AIM:To investigate morphological changes in the cornea and meibomian glands using in vivo confocal microscopy(IVCM)in patients with type 2 diabetes(T2DM)and to analyze the correlation of these changes with disease duration(DD).METHODS:Patients with T2DM who visited the ophthalmology and endocrinology departments of our hospital from May 2023 to December 2023 were selected.According to DD,they were divided into the short-DD(≤10y)and long-DD(>10y)groups.The control group consisted of age-matched non-diabetes patients.All underwent Ocular Surface Disease Index(OSDI),tear break-up time(TBUT),Schirmer Ⅰ test(SIT),and IVCM imaging.Corneal nerve parameters,including corneal nerve fiber density(CNFD),corneal nerve branch density(CNBD),corneal nerve fiber length(CNFL),and meibomian gland metrics,including density of meibomian gland acinar units(MGAUD),the longest diameter of the meibomian gland acini(MGALD),the shortest diameter of meibomian gland acini(MGASD),the area of meibomian gland acinar units(MGAUA),were analyzed using ImageJ and ACCMetrics.RESULTS:A total of 130 patients with T2DM were included in this study,among which 68 were male(52.3%,age 30-76y)and 62 were female(47.7%,age 30-76y),the average age of the group was 55.54±11.65y.Significant differences(P0.05).CONCLUSION:T2DM patients exhibit greater corneal nerve and meibomian gland damage than age 30-76y nontype 2 diabetes patients.Prolonged DD exacerbates these morphological changes. 展开更多
关键词 diabetes corneal nerves meibomian glands in vivo confocal microscopy ocular surface
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Challenges in piezoelectric force microscopy characterization of sliding ferroelectric WTe2 认领 引用
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作者 Yichen Sun Luqi Wei +11 位作者 Wencheng Fan Weihao Sun Haonan Wang Yaqiong Wang Zhao Guan Wenyi Tong Ke Qu Zhenzhong Yang Binbin Chen Pinghua Xiang Chungang Duan Ni Zhong 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第7期198-205,共8页
Sliding ferroelectricity,an emerging ferroelectric mechanism in 2D systems whose polarization arises from interlayer charge transfer,has attracted considerable attention due to its unique switching behavior,such as su... Sliding ferroelectricity,an emerging ferroelectric mechanism in 2D systems whose polarization arises from interlayer charge transfer,has attracted considerable attention due to its unique switching behavior,such as superior endurance and high switching speed.However,its experimental characterization remains challenging due to the low polarizability and technical difficulties.Using piezoelectric force microscopy(PFM),we investigate Td-WTe2 as a model sliding ferroelectric system,aiming to fill the gap in the characterization of sliding ferroelectricity from a microscopic perspective.We observed ferroelectric-like PFM phase contrast and amplitude hysteresis in few-layer(1-4 L)WTe2 flakes under ambient conditions,in contrast to the theoretical predictions for flakes thicker than 2 L.Surface oxidation of WTe2 has been identified by Raman and other characterization techniques.Therefore,similar PFM characterization has been carried out on the flakes covered by h-BN,which prevented WTe2 from oxidation,and no ferroelectric behavior could be found.Consequently,the ferroelectric-like PFM phenomena are attributed to oxidation rather than intrinsic ferroelectricity in WTe2.Combined with analyses of PFM testing principles and the mechanism of sliding ferroelectricity,we identify the limitations and challenges of PFM in characterizing sliding ferroelectricity.This work provides valuable insights for the subsequent characterization and application of related sliding ferroelectrics. 展开更多
关键词 2D ferroelectric sliding ferroelectric piezoelectric force microscopy(PFM)
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基于FEM-PFM的并联空间管路系统振动传递分析 认领 引用
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作者 刘宝繁 孙伟 吕尚 《振动与冲击》 EI CSCD 北大核心 2026年第9期137-145,共9页
在外激励作用下振动会沿着航空发动机管路进行传递,获取航空发动机管路的振动传递规律有助于指导管路动力学设计以提升管路系统的抗振性。以并联空间管路为对象,将功率流法和有限元法相结合,提出了单元级的振动传递可视化方法,发现了丰... 在外激励作用下振动会沿着航空发动机管路进行传递,获取航空发动机管路的振动传递规律有助于指导管路动力学设计以提升管路系统的抗振性。以并联空间管路为对象,将功率流法和有限元法相结合,提出了单元级的振动传递可视化方法,发现了丰富的能量传递行为并从模态的角度揭示了振动传递机理,然后对ANSYS软件进行了二次开发使其具有功率流分析与振动传递可视化功能。在以梁单元为管体有限元建模的基础上,详细给出了模拟管接头、单联卡箍和双联卡箍的质量及刚度的方法,最终形成了便于后续振动传递分析的并联空间管路有限元模型。进一步,描述了基于ANSYS软件计算节点功率流以及振动传递方向的方法同时给出了实现振动传递可视化的流程。最后,进行了实例研究,用组建的试验系统校验了所创建的并联空间管路的合理性,在此基础上分析了并联管路系统在第2阶共振状态下的振动传递规律。结果表明:功率流在空间管路折弯区域、单联卡箍和双联卡箍支撑区域附近可能存在突变现象,进而可能改变振动传递的方向,系统的模态位移和模态应力极值区域可能是振源也可能是振汇。 展开更多
关键词 并联空间管路 有限元法(FEM) 功率流法(PFM) 振动传递分析 振动传递可视化
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Advances in deep learning for bacterial image segmentation in optical microscopy 认领 引用
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作者 Zhijun Tan Yang Ding +6 位作者 Huibin Ma Jintao Li Danrou Zheng Hua Bai Weini Xin Lin Li Bo Peng 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2026年第1期30-44,共15页
Microscopy imaging is fundamental in analyzing bacterial morphology and dynamics,offering critical insights into bacterial physiology and pathogenicity.Image segmentation techniques enable quantitative analysis of bac... Microscopy imaging is fundamental in analyzing bacterial morphology and dynamics,offering critical insights into bacterial physiology and pathogenicity.Image segmentation techniques enable quantitative analysis of bacterial structures,facilitating precise measurement of morphological variations and population behaviors at single-cell resolution.This paper reviews advancements in bacterial image segmentation,emphasizing the shift from traditional thresholding and watershed methods to deep learning-driven approaches.Convolutional neural networks(CNNs),U-Net architectures,and three-dimensional(3D)frameworks excel at segmenting dense biofilms and resolving antibiotic-induced morphological changes.These methods combine automated feature extraction with physics-informed postprocessing.Despite progress,challenges persist in computational efficiency,cross-species generalizability,and integration with multimodal experimental workflows.Future progress will depend on improving model robustness across species and imaging modalities,integrating multimodal data for phenotype-function mapping,and developing standard pipelines that link computational tools with clinical diagnostics.These innovations will expand microbial phenotyping beyond structural analysis,enabling deeper insights into bacterial physiology and ecological interactions. 展开更多
关键词 Bacterial image deep learning optical microscopy image segmentation artificial intelligence
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Instantaneous desulfurization of molten steel with varied aluminum and silicon by CaO-Al2O3 particles:In situ observation using confocal scanning laser microscopy 认领 引用
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作者 Chunjie She Hejun Zhang +2 位作者 Yanhui Zhang Ying Ren Lifeng Zhang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2026年第2期545-554,共10页
Desulfurization of CaO–Al2O3 particles in molten steel was observed in situ using high-temperature confocal scanning laser microscopy.The effects of the aluminum and silicon contents of molten steel on desulfur... Desulfurization of CaO–Al2O3 particles in molten steel was observed in situ using high-temperature confocal scanning laser microscopy.The effects of the aluminum and silicon contents of molten steel on desulfurization were analyzed.When the total aluminum content in the steel increased from 6 to 1100 ppm,the CaS content in CaO–Al2O3 particles increased from 2.1wt%to 84.84wt%after the reaction for 90 s.Furthermore,when the silicon content in the steel increased from 0.01wt%to 2.20wt%,the CaS content in CaO–Al2O3 particles increased from 1.53wt%to 79.01wt%after the reaction for 90 s.This indicates that the increase in the aluminum and silicon contents of the steel promoted the desulfurization of CaO–Al2O3 particles.A kinetic model was established to predict the CaO–Al2O3 particles composition,and the diffusion coefficient of sulfur in CaO–Al2O3 particles was 9.375×10−10m2·s−1 at 1600℃,which provided a new method for the calculation of diffusion coefficient. 展开更多
关键词 desulfurization high-temperature confocal scanning laser microscopy steel desulfurizer kinetic model aluminum silicon
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DenT:Dense-Transformer for Label-Free Microscopy Image Segmentation 认领 引用
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作者 Chan-Min Hsu Shang-Ru Yang +1 位作者 Yi-Ju Lee An-Chi Wei 《Computers, Materials & Continua》 SCIE EI 2026年第7期278-293,共16页
U-Net,a fully convolutional neural network(FCNN)with U-shaped features,has demonstrated significant success in biomedical image segmentation.However,the locality of convolution operations in the U-Net limits its abili... U-Net,a fully convolutional neural network(FCNN)with U-shaped features,has demonstrated significant success in biomedical image segmentation.However,the locality of convolution operations in the U-Net limits its ability to learn long-range dependencies.Transformers,originally developed for natural language processing,have recently been adapted for image segmentation because of their global self-attention mechanisms.Inspired by the long-range feature learning capability of transformers,we propose Dense-Transformer(DenT),an architecture designed for volumetric microscopy image segmentation.DenT incorporates transformers as encoders within each convolutional layer to capture global contextual information.Additionally,dense skip connections at multiple resolutions enhance feature propagation,enabling precise localization.We evaluated DenT on mitochondrial segmentation using our confocal microscopy dataset and a public fluorescence microscope dataset from the Allen Institute for Cell Science.The experimental results demonstrate that DenT incrementally improves the segmentation of mitochondria and mitochondrial DNA substructures from transmitted light microscopy images.DenT offers a tool for visualization,measurement,and analysis of mitochondrial morphology and mitochondrial DNA in label-free microscopy. 展开更多
关键词 UNet transformer image segmentation mitochondrial organelle deep learning microscopy imaging
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Structural anisotropy in celery stalks by microscopic MRI and polarized light microscopy 认领 引用
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作者 Sarah Salem Farid Badar +3 位作者 Amanveer Singh Vladimir Sokolovsky Gregory Furman Yang Xia 《Magnetic Resonance Letters》 EI CAS 2026年第2期80-86,共7页
Structural anisotropy of celery stalks was studied using T2 anisotropy in microscopic MRI(μMRI)and supplemented by quantitative polarized light microscopy(PLM)at optical resolutions.Parenchyma,which is the ground ... Structural anisotropy of celery stalks was studied using T2 anisotropy in microscopic MRI(μMRI)and supplemented by quantitative polarized light microscopy(PLM)at optical resolutions.Parenchyma,which is the ground tissue in celery and has a diameter in the range of 50–90μm,was found to have isotropic T2;in contrast,collenchyma,which is the structural part of the ground tissue in celery and has a diameter in the range of 8–12μm,was found to have strong anisotropic T2.Substantial size variations within each porous structure and substantial co-existences of more than one type of structural tissues within a singleμMRI voxel were noticed in the optical images,which can contribute to the less clear anisotropies in the smaller vascular structures(e.g.,phloem(approximately 2–6μm in diameter)and xylem(approximately 5–15μm in diameter)).Celery could be used as a simple plant model to study the relationships between tissue microstructures and nuclear spin relaxation in fibrous and porous specimens. 展开更多
关键词 T2anisotropy Celery Microscopic resolution Polarized light microscopy
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Microscopy and molecular approaches:A combined diagnostic framework for malaria surveillance in Malaysia 认领 引用
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作者 Kumuthamalar Sangaran Si Jie Goh +2 位作者 Ameera Aleeshya Mohd Fadrul Izwan Guan Min Wee Zulkarnain Md Idris 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2026年第5期193-195,共3页
By 2024,Malaysia achieved a significant public health milestone by marking its seventh consecutive year without a single local transmission of indigenous human Plasmodium species.The country has sustained elimination ... By 2024,Malaysia achieved a significant public health milestone by marking its seventh consecutive year without a single local transmission of indigenous human Plasmodium species.The country has sustained elimination of non-zoonotic malaria,primarily caused by Plasmodium(P.)falciparum and P.vivax,while remaining vigilant against imported and introduced cases driven by cross-border movement and international travel.However,despite this achievement,Malaysia has emerged as the global epicentre of zoonotic for P.knowlesi. 展开更多
关键词 indigenous human plasmodium species p vivax Malaysia malaria surveillance molecular approaches elimination microscopy imported cases
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The Serendipitous Rise of Mid-infrared Photothermal Microscopy 认领 引用
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作者 Xinyu Deng Hyeon Jeong Lee Delong Zhang 《Chemical & Biomedical Imaging》 CAS CSCD 2026年第1期1-5,共5页
The recent Nature Methods cover featuresafocusissueon bond-selective imaging,1−7 celebrating the renaissance of vibrational microscopy and highlighting how chemical bond vibrations have emerged as powerful reporters o... The recent Nature Methods cover featuresafocusissueon bond-selective imaging,1−7 celebrating the renaissance of vibrational microscopy and highlighting how chemical bond vibrations have emerged as powerful reporters of molecular identity and function in living systems.When we talk about“bond”,it more often come to mind of another Bond,James Bond.Here’s a brain teaser:What do James Bond and a chemical bond have in common?Both are excellent agents for extracting information.Why? 展开更多
关键词 mid infrared photothermal microscopy serendipitous rise chemical bond vibrational microscopy extracting informationwhy james bond bond selective imaging brain teaser what
Self-supervised denoising strategies for multimodality light-field microscopy 认领 引用
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作者 Xuyu Zeng Xingye Chen +2 位作者 Zhifeng Zhao Yuanlong Zhang Fei Dai 《Advanced Photonics Nexus》 CSCD 2026年第5期57-68,共12页
Light-field fluorescence microscopy(LFM)enables high-speed volumetric imaging by capturing 3D information in a single snapshot.However,its reconstruction quality is severely degraded by detection noise.We propose SSD-... Light-field fluorescence microscopy(LFM)enables high-speed volumetric imaging by capturing 3D information in a single snapshot.However,its reconstruction quality is severely degraded by detection noise.We propose SSD-LFM,a self-supervised denoising framework that incorporates physical models,to substantially remove noise-induced artifacts in multimodality LFM.The SSD-LFM model is trained exclusively on low-signal-to-noise ratio(SNR)data,bypassing the need for paired noisy-clean datasets for network training.The denoising performance of SSD-LFM is validated on conventional LFM,scanning LFM(sLFM),and two-photon excitation LFM(TP-LFM).Comprehensive evaluations on both simulated and experimental data demonstrate that SSD-LFM achieves artifact-free 3D reconstruction with over 5-fold fewer photons.For time-lapse imaging,the self-supervised architecture further enables SSD-LFM to operate only on the collected noisy raw data,overcoming generalization constraints inherent in supervised methods.Due to the significantly improved quality under low-SNR excitation,SSD-LFM enables long-term volumetric observation of phototoxicity-vulnerable bioprocesses. 展开更多
关键词 light-field microscopy imaging denoising self-supervised learning
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Tunable waist control in axially swept light-sheet microscopy for optimized signal and axial resolution 认领 引用
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作者 Nikhil Sain Ellie S.Kennedy +3 位作者 Muthubharathi B.Chellammal Rita E.Serda Olga Ponomarova Tonmoy Chakraborty 《Advanced Photonics Nexus》 CSCD 2026年第4期90-100,共11页
Ever since its inception,axially swept light-sheet microscopy(ASLM)has demonstrated its diverse imaging capability,particularly in the context of large,cleared specimens.Yet current implementations face fundamental li... Ever since its inception,axially swept light-sheet microscopy(ASLM)has demonstrated its diverse imaging capability,particularly in the context of large,cleared specimens.Yet current implementations face fundamental limitations in optimizing imaging performance for heterogeneous samples.Although significant advances have been made in ASLM development,obtaining sufficient signal while maintaining optimal axial resolution remains a persistent challenge.Moreover,current ASLM implementations use fixed illumination numerical apertures(NAs)or lossy mechanical slits,preventing dynamic light-sheet waist control,thereby restricting multiscale imaging flexibility.Here,we present an ASLM-based optical method that enhances sample signal detection without the need to increase laser power while providing flexibility in axial resolution control ranging from 500 nm to 1.6μm.Our approach enables flexible and lossless control of light-sheet thickness in the sample space to achieve variable axial resolution within the specified range.This tunable beam shaping capability addresses the fundamental limitations of current ASLM systems by providing researchers with the ability to optimize imaging parameters by bridging the critical gap between resolution and signal in ASLM-based microscopy.We demonstrate the utility of our approach by imaging fluorescent beads and various cleared-tissue samples. 展开更多
关键词 biomedical imaging microscopy tissue clearing techniques photonics light
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Seeing the Unseen:Super-Resolution Microscopy in Protein Aggregation Research 认领 引用
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作者 Molly J.M.Turner Junsheng Chen +1 位作者 Nikos S.Hatzakis Min Zhang 《Chemical & Biomedical Imaging》 CAS CSCD 2026年第2期116-129,共14页
Super-resolution microscopy surpasses the diffraction limit and enables the visualization of biomolecular structures with unprecedented detail.These techniques have been widely used in many scientific areas,including ... Super-resolution microscopy surpasses the diffraction limit and enables the visualization of biomolecular structures with unprecedented detail.These techniques have been widely used in many scientific areas,including cell biology,genomics,microbiology,and material science.In the field of protein aggregation,a process intimately linked to numerous neurodegenerative diseases,the high spatial resolution of super-resolution microscopy enables the direct observation of the fine structure of different species,ranging from small oligomers to mature aggregates,providing insights into molecular aggregation mechanisms and the pathology of neurodegenerative diseases,such as Parkinson’s,Alzheimer’s,and Huntington’s disease.In this review,we outline the principles of three major super-resolution microscopy techniques,including stimulated emission depletion(STED),structured illumination microscopy(SIM),and single-molecule localization microscopy(SMLM),and compare their respective strengths and limitations in studying protein aggregation.We then highlight the recent applications of these techniques in studying protein aggregation,with a focus on aggregate morphology,dynamic formation processes,and interactions with cellular components. 展开更多
关键词 super-resolution microscopy protein aggregation neurodegenerative disease morphology aggregation pathways distribution toxicity pathology
High-fidelity microsecond-scale cellular imaging using two-axis compressed streak imaging fluorescence microscopy 认领 引用
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作者 Mark A.Keppler Sean P.O’Connor +4 位作者 Zachary A.Steelman Xianglei Liu Jinyang Liang Vladislav V.Yakovlev Joel N.Bixler 《Advanced Photonics Nexus》 CSCD 2026年第4期185-201,共17页
Compressed streak imaging(CSI)is a computational imaging strategy that can acquire video at over 150 trillion frames per second.Despite this achievement,CSI faces challenges in detecting subtle intensity fluctuations ... Compressed streak imaging(CSI)is a computational imaging strategy that can acquire video at over 150 trillion frames per second.Despite this achievement,CSI faces challenges in detecting subtle intensity fluctuations in slow-moving,continuously illuminated objects.This limitation,largely attributable to high streak compression and motion blur,has curtailed the broader adoption of CSI in cellular fluorescence microscopy.To address these issues and expand the utility of CSI,we developed a two-axis CSI(TACSI)method that results in significant improvements to the reconstructed video fidelity.TACSI introduces a second scanning axis,which shuttles a conjugate image of the object with respect to the coded aperture(CA).The moving image decreases the localized spatiotemporal mixing and produces a“flash and shutter”phenomenon that reduces CA motion blur,overcoming the limitations of current CSI technologies.This approach is supported by an analytical model describing the TACSI compression ratio,along with simulated and empirical measurements.We demonstrate TACSI’s ability tomeasure rapid variations in cellmembrane potentials,previously unattainable with conventional CSI.This method has broad implications for high-speed photography,including visualization of action potentials,muscle contractions,and enzymatic reactions that occur on microsecond and faster timescales using fluorescence microscopy. 展开更多
关键词 compressed sensing streak photography fluorescence microscopy high-speed imaging cellular biology electrophysiology
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Visualizing Single Molecular Crystals by Electrochemiluminescence Microscopy 认领 引用
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作者 Yufei Wang Jianping Lei 《Chemical & Biomedical Imaging》 CAS CSCD 2026年第2期113-115,共3页
Electrochemiluminescence(ECL)is a light-emitting process driven by electrochemical reactions near the electrode surface,first pioneered by Bard's group in 2002.1 Due to the absence of external light excitation,the... Electrochemiluminescence(ECL)is a light-emitting process driven by electrochemical reactions near the electrode surface,first pioneered by Bard's group in 2002.1 Due to the absence of external light excitation,the ECL technique enables an excitation-emission decoupling capa-bility with remarkable advantages such as near-zero back-ground,high sensitivity,and controllable spatial resolution.By integrating high-resolution microscopy with ECL signal transduction,ECL microscopy originating directly from electrochemical reactions serves as a spatiotemporal imaging tool for studying ECL reaction kinetics and visualizing single particles,even down to single molecules. 展开更多
关键词 microscopy spatial resolution sensitivity single molecular crystals electrochemiluminescence electrochemical reactions electrode surfacefirst reaction kinetics
Role of transmission electron microscopy in heterogeneous structural nanomaterials for photocatalytic applications 认领 引用 被引量：1
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作者 Chunqiang Zhuang Can Zhang +6 位作者 Dong Zhang Yihong Zhang Zizhao Liu Peng Shan Wei Zhang Peng Xu Shijie Li 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2025年第36期195-233,共39页
The photocatalytic activity of a photocatalyst is significantly influenced by its microstructure.Therefore,it is crucial to understand and characterize the microstructure.Transmission electron microscopy(TEM)has been ... The photocatalytic activity of a photocatalyst is significantly influenced by its microstructure.Therefore,it is crucial to understand and characterize the microstructure.Transmission electron microscopy(TEM)has been an indispensable tool that provides direct visualization of the microstructure at the atomic level.Despite numerous reviews on photocatalytic processes,there has been a lack of comprehensive summaries that focus on the critical role of TEM in photocatalytic applications.This review aims to fill this gap by highlighting the significant contributions of TEM techniques in understanding key photocatalytic processes,including light absorption,charge separation and transfer,and surface reaction.In addition,this review discusses in-situ TEM techniques for observing photocatalytic reactions and explores the challenges and future perspectives related to the application of TEM in photocatalysis. 展开更多
关键词 Photocatalytic reaction Photocatalyst design Transmission electron microscopy In-situ transmission electron microscopy
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In-situ observation of nonmetallic inclusions in steel using confocal scanning laser microscopy:A review 认领 引用 被引量：4
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作者 Ying Ren Lifeng Zhang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2025年第5期975-991,共17页
The characteristics of nonmetallic inclusions formed during steel production have a significant influence on steel performance.In this paper,studies on inclusions using confocal scanning laser microscopy(CSLM)are revi... The characteristics of nonmetallic inclusions formed during steel production have a significant influence on steel performance.In this paper,studies on inclusions using confocal scanning laser microscopy(CSLM)are reviewed and summarized,particularly the col-lision of various inclusions,dissolution of inclusions in liquid slag,and reactions between inclusions and steel.Solid inclusions exhibited a high collision tendency,whereas pure liquid inclusions exhibited minimal collisions because of the small attraction force induced by their<90°contact angle with molten steel.The collision of complex inclusions in molten steel was not included in the scope of this study and should be evaluated in future studies.Higher CaO/Al2O3and CaO/SiO2ratios in liquid slag promoted the dissolution of Al2O3-based in-clusions.The formation of solid phases in the slag should be prevented to improve dissolution of inclusions.To accurately simulate the dissolution of inclusions in liquid slag,in-situ observation of the dissolution of inclusions at the steel-slag interface is necessary.Using a combination of CSLM and scanning electron microscopy-energy dispersive spectroscopy,the composition and morphological evolution of the inclusions during their modification by the dissolved elements in steel were observed and analyzed.Although the in-situ observa-tion of MnS and TiN precipitations has been widely studied,the in-situ observation of the evolution of oxide inclusions in steel during so-lidification and heating processes has rarely been reported.The effects of temperature,heating and cooling rates,and inclusion character-istics on the formation of acicular ferrites(AFs)have been widely studied.At a cooling rate of 3-5 K/s,the order of AF growth rate in-duced by different inclusions,as reported in literature,is Ti-O<Ti-Ca-Zr-Al-O<Mg-O<Ti-Zr-Al-O<Mn-Ti-Al-O<Ti-Al-O<Zr-Ti-Al-O.Further comprehensive experiments are required to investigate the quantitative relationship between the formation of AFs and inclusions. 展开更多
关键词 inclusion steel in-situ observation confocal scanning laser microscopy
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Fusion-based enhancement of multi-exposure Fourier ptychographic microscopy 认领 引用 被引量：2
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作者 Zhiping Wang Tianci Feng +2 位作者 Aiye Wang Jinghao Xu An Pan 《Advanced Photonics Nexus》 CSCD 2025年第4期1-11,共11页
Fourier ptychographic microscopy(FPM)is an innovative computational microscopy approach that enables high-throughput imaging with high resolution,wide field of view,and quantitative phase imaging(QPI)by simultaneously... Fourier ptychographic microscopy(FPM)is an innovative computational microscopy approach that enables high-throughput imaging with high resolution,wide field of view,and quantitative phase imaging(QPI)by simultaneously capturing bright-field and dark-field images.However,effectively utilizing dark-field intensity images,including both normally exposed and overexposed data,which contain valuable high-angle illumination information,remains a complex challenge.Successfully extracting and applying this information could significantly enhance phase reconstruction,benefiting processes such as virtual staining and QPI imaging.To address this,we introduce a multi-exposure image fusion(MEIF)framework that optimizes dark-field information by incorporating it into the FPM preprocessing workflow.MEIF increases the data available for reconstruction without requiring changes to the optical setup.We evaluate the framework using both feature-domain and traditional FPM,demonstrating that it achieves substantial improvements in intensity resolution and phase information for biological samples that exceed the performance of conventional high dynamic range(HDR)methods.This image preprocessing-based information-maximization strategy fully leverages existing datasets and offers promising potential to drive advancements in fields such as microscopy,remote sensing,and crystallography. 展开更多
关键词 Fourier ptychographic microscopy multi-exposure image fusion computational imaging feature-domain nonlinear image fusion
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A promising approach for quantifying focal stroke modeling and assessing stroke progression:optical resolution photoacoustic microscopy photothrombosis 认领 引用 被引量：2
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作者 Xiao Liang Xingping Quan +6 位作者 Xiaorui Geng Yujing Huang Yonghua Zhao Lei Xi Zhen Yuan Ping Wang Bin Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2025年第7期2029-2037,共9页
To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these me... To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly age-related.In this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed laser.We observed the molding process in mice of different ages and presented age-dependent vascular embolism differentiation.Moreover,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a stroke.Our imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes. 展开更多
关键词 age-dependent cerebral cortex ischemic stroke mouse model optical coherence tomography angiography photoacoustic microscopy photothrombosis vascular imaging
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Characteristics and differential diagnosis of common verrucous proliferative skin diseases under dermoscopy and reflectance confocal microscopy 认领 引用 被引量：2
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作者 ZHOU Lu FU Yule +7 位作者 HUANG Jian TANG Zhen LU Jianyun TAN Lina WANG Dan ZENG Jinrong WANG Jia GAO Lihua 《中南大学学报（医学版）》 CAS CSCD 北大核心 2025年第3期358-365,共8页
Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often ... Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often posing diagnostic challenges.Dermoscopy and reflectance confocal microscopy(RCM)can aid in their differentiation,yet their specific features under these tools have not been systematically described.This study aims to summarize and analyze the dermoscopic and RCM features of VEN,SK,VP,VV,and NS.Methods:A total of 121 patients with histopathologically confirmed verrucous proliferative skin diseases were enrolled.Dermoscopy and RCM imaging was used to observe and analyze the microscopic features of these conditions.Results:Under dermoscopy,the 5 diseases displayed distinct characteristics:VEN typically showed gyriform structures;SK was characterized by gyriform structures,comedo-like openings,and milia-like cysts;VP and VV featured dotted vessels and frogspawn-like structures;NS presented as brownish-yellow globules.RCM revealed shared features such as hyperkeratosis and acanthosis across all 5 diseases.Specific features included gyriform structures and elongated rete ridges in VEN;pseudocysts and gyriform structures in SK;evenly distributed ring-like structures in VP;vacuolated cells and papillomatous proliferation in VV;and frogspawn-like structures in NS.Conclusion:These 5 verrucous proliferative skin conditions exhibit distinguishable features under both dermoscopy and RCM.The combination of these 2 noninvasive imaging modalities holds significant clinical value for the differential diagnosis of verrucous proliferative skin diseases. 展开更多
关键词 reflectance confocal microscopy dermoscopy verrucous proliferation verrucous epidermal nevus seborrheic keratosis verruca plana verruca vulgaris nevus sebaceous
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