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Comprehensive characterization of lncRNA N6-methyladenosine modification dynamics throughout bovine skeletal muscle development 认领 引用 被引量：2
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作者 Cui Mao Wei You +4 位作者 Yuta Yang Haijian Cheng Xin Hu Xianyong Lan Enliang Song 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第3期1025-1040,共16页
Background N6-methyladenosine(m6A)methylation is a key epigenetic modification that can modulate gene expression and strongly affect mammalian developmental processes.However,the genome-wide methylation of long ... Background N6-methyladenosine(m6A)methylation is a key epigenetic modification that can modulate gene expression and strongly affect mammalian developmental processes.However,the genome-wide methylation of long non-coding RNAs(lncRNAs)and its implications for the development of skeletal muscle remain poorly understood.Bovine skeletal muscle samples from five developmental stages were analyzed in this study to establish lncRNA methylome and transcriptomic maps.Results Globally,59.67%of lncRNAs in skeletal muscle with m6A modifications,and this percentage decreased progressively during development.lncRNA expression levels were positively associated with the number of m6A peaks,with lncRNAs possessing 3 or more peaks showing significantly higher expression levels than those with 1 or 2 peaks.Specific lncRNAs involved in skeletal muscle development were identified through two analytical approaches.The first approach employed weighted gene co-expression network analysis(WGCNA)of transcriptomic data to identify correlations between annotated lncRNAs and growth-related traits,resulting in 21 candidate hub lncRNAs.The intersection of these 21 hub lncRNAs with 151 differentially methylated lncRNAs(DM-lncRNAs)identified 10 shared candidate lncRNAs.The second approach integrated MeRIP-seq and RNA-seq data to identify 36 lncRNAs that were both differentially m6A modified and differentially expressed(dme-lncRNAs).GO and KEGG enrichment analyses of cis-target genes associated with these dme-lncRNAs identified eight candidate lncRNAs.Combining the results from the two approaches identified 16 key m6A-modified lncRNAs likely involved in skeletal muscle development.Conclusions These findings highlight the regulatory and functional significance of dynamic lncRNA methylation in skeletal muscle development. 展开更多
关键词 Bovine LncRNA m6A methylation Muscle development
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Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development 认领 引用 被引量：8
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作者 YANG Ya-lan LI Yan +6 位作者 LIANG Ru-yi ZHOU Rong AO Hong MU Yu-lian YANG Shu-lin LI Kui TANG Zhong-lin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第6期1331-1339,共9页
MicroRNAs （miRNAs）, evolutionarily conserved non-coding RNAs in length 21-24 bp, play a critical role in skeletal muscle development. In this study, to explore the function of mircoRNA-127 in porcine skeletal muscle... MicroRNAs （miRNAs）, evolutionarily conserved non-coding RNAs in length 21-24 bp, play a critical role in skeletal muscle development. In this study, to explore the function of mircoRNA-127 in porcine skeletal muscle development, eight tissue samples from adult pigs and longissimus muscle samples at 26 developmental stages were collected from Tongcheng and Landrace pigs. The spatial-temporal expression proifles of miRNA-127 were carried out using step-loop quantitative real-time PCR （stem-loop RT-PCR）. To explore the molecular functions of miRNA-127, we predicted its target genes and performed functional annotation using bioinformatics methods. Results suggested that miRNA-127 was abundantly expressed in heart, ovary, uterus and spleen tissues and was weakly expressed in liver, lung, kidney and small intestine in both Tongcheng and Landrace pigs. And miRNA-127 showed signiifcant expression differences in heart, ovary, spleen and uterus tissues between these two breeds. miRNA-127 basically kept at a relatively stable high level in middle and later embryonic stages and a low expression level in early embryonic stages and postnatal stages, but the expression levels of miRNA-127 were higher in Tongcheng pigs than in Landrace at most developmental stages. miRNA-127 potentially regulated 240 candidate genes. Results of Gene Ontology and KEGG pathway analysis indicated that these genes could be involved in many molecular functions and mechanisms, such as regulation of the force of heart contraction, regulation of transcription, regulation of T cell differentiation, MAPK signaling pathway and GnRH signaling pathway. Many signiifcantly enriched GO terms and KEGG pathways were related to skeletal muscle development. This study will be helpful to understand the biological function for miRNA-127 and identify candidate gene associated with meat production traits in pigs. 展开更多
关键词 porcine microRNA-127 expression profiles skeletal muscle development target prediction
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Vitamin A regulates intramuscular adipose tissue and muscle development:promoting high-quality beef production 认领 引用 被引量：14
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作者 Dong Qiao Peng Stephen B.Smith Hong Gu Lee 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第3期1138-1147,共10页
During growth in cattle,the development of intramuscular adipose tissue and muscle is dependent upon cell hyperplasia(increased number of adipocytes)and hypertrophy(increased size of adipocytes).Based on the results o... During growth in cattle,the development of intramuscular adipose tissue and muscle is dependent upon cell hyperplasia(increased number of adipocytes)and hypertrophy(increased size of adipocytes).Based on the results of previous studies,other adipose tissue depots(e.g.,perirenal and subcutaneous)develop from the fetal stage primarily as brown adipose tissue.The hyperplastic stage of intramuscular adipose is considered to develop from late pregnancy,but there is no evidence indicating that intramuscular adipose tissue develops initially as brown adipose tissue.Hyperplastic growth of intramuscular adipose continues well into postweaning and is dependent on the timing of the transition to grain-based diets;thereafter,the late-stage development of intramuscular adipose tissue is dominated by hypertrophy.For muscle development,hyperplasia of myoblasts lasts from early(following development of somites in the embryo)to middle pregnancy,after which growth of muscle is the result of hypertrophy of myofibers.Vitamin A is a fat-soluble compound that is required for the normal immunologic function,vision,cellular proliferation,and differentiation.Here we review the roles of vitamin A in intramuscular adipose tissue and muscle development in cattle.Vitamin A regulates both hyperplasia and hypertrophy in in vitro experiments.Vitamin A supplementation at the early stage and restriction at fattening stage generate opposite effects in the beef cattle.Appropriate vitamin A supplementation and restriction strategy increase intramuscular adipose tissue development(i.e.,marbling or intramuscular fat)in some in vivo trials.Besides,hyperplasia and hypertrophy of myoblasts/myotubes were affected by vitamin A treatment in in vitro trials.Additionally,some studies reported an interaction between the alcohol dehydrogenase-1C(ADH1C)genotype and vitamin A feed restriction for the development of marbling and/or intramuscular adipose tissue,which was dependent on the timing and level of vitamin A restriction.Therefore,the feed strategy of vitamin A has the visible impact on the marbling and muscle development in the cattle,which will be helpful to promote the quality of the beef. 展开更多
关键词 Adipose tissue Cattle Intramuscular adipose tissue Muscle development Vitamin A
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Modified halloysite nanotubes reduce the toxic effects of zearalenone in gestating sows on growth and muscle development of their offsprings 认领 引用 被引量：6
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作者 Rui Gao Qingwei Meng +4 位作者 Jianan Li Min Liu Yuanyuan Zhang Chongpeng Bi Anshan Shan 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2016年第4期570-578,共9页
Background:Zearalenone(ZEN)is an estrogenic mycotoxin that is primarily produced by Fusarium fungi and has been proven to affect the reproductive capacity of many species to varying degrees.The present experiment was ... Background:Zearalenone(ZEN)is an estrogenic mycotoxin that is primarily produced by Fusarium fungi and has been proven to affect the reproductive capacity of many species to varying degrees.The present experiment was designed to study the maternal persistent effects of zearalenone toxicity in gestating sows on growth and muscle development of their offsprings,and the alleviation of zearalenone toxicity by modified halloysite nanotubes(MHNTs).Methods:Eighteen sows were fed with one of three dietary treatments that included the following:(1)a control diet,(2)a contaminated grain diet(with 50%moldy corn,2.77 mg/kg ZEN),and(3)a contaminated grain diet(with 50%moldy corn,2.76 mg/kg ZEN)+1%MHNTs.Each sow was exclusively fed its experimental diets from 35 to 70 d of gestation at a total of 2 kg daily.Muscle samples were collected from six piglets per treatment at birth,weaning and finishing.Results:The results showed that feeding the sows with the ZEN-contaminated diets from 35 to 70 d of gestation decreased the ADG,ADFI and G:F of their offsprings(P〈0.05).The muscle fiber numbers in the newborn,weaning and growing-finishing pigs and the muscle fiber diameters at birth and weaning were also decreased by maternal ZEN exposure(P〈0.05).The expressions of IGF-I,IGF-II,Myf-5 and Mstn at birth and IGF-II,Pax7,Myf-5 and MyoD1 at weaning were altered by feeding gestating sows with ZEN-contaminated diets(P〈0.05).The MHNTs reduced most of the ZEN-induced toxic effects:the ADG and ADFI on growth performance,the muscle fiber numbers at weaning and finishing and the muscle fiber diameters at weaning(P〈0.05).The expression levels of IGF-II and Mstn in newborn piglets and IGF-II and Myf-5 in weaning piglets were also prevented by adding 1%MHNTs(P〈0.05).Conclusions:The present study demonstrated that the offsprings of sows fed with ZEN-contaminated diets from 35 to 70 day of gestation exhibited weakening on growth performance,physiological changes in their muscle fibers and alterations of mRNA expression in their muscle tissues,and also indicated that MHNTs prevented most of the ZEN-induced weakening in the muscle tissues. 展开更多
关键词 Growth MHNTs Muscle development Offsprings Sows Zearalenone
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A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats 认领 引用 被引量：4
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作者 Ying Yuan Wei-Yi Zhang +28 位作者 Bai-Gao Yang Dong-Ke Zhou Lu Xu Yong-Meng He Hao-Yuan Zhang Cheng-Li Liu Yue-Hui Ma Ming-Xing Chu Wen-Guang Zhang Hui-Jiang Gao Lin Jiang Fu-Ping Zhao Lu-Pei Zhang Ri-Su Na Baatarchogt Oyungerel Yan-Guo Han Yan Zeng Shi-Zhi Wang Huai-Zhi Jiang Hong-Ping Zhang Xun-Ping Jiang Jian-Ning He Hao Liang Kaushalendra Kaushalendra Ya-Wang Sun Yong-Fu Huang Yong-Ju Zhao Zhong-Quan Zhao Guang-Xin E 《Zoological Research》 SCIE CSCD 2023年第2期303-314,共12页
The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains ... The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance.However,the genetic basis of muscle development in the Boer goat remains obscure.In this study,we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development.A total of9 959 autosomal copy number variations(CNVs) were identified through selection signal analysis in 127 goat genomes.Specifically,we confirmed that the highest signal CNV(HSV) was a chromosomal arrangement containing an approximately 1.11 Mb(CHIR17:60062304-61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region(CHIR17:60145940-60151302 bp) with overlapping genes(e.g.,ARHGAP10,NR3C2,EDNRA,PRMT9,and TMEM184C).The homozygous duplicated HSV genotype(+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African goats.The expression network of three candidate genes(ARHGAP10,NR3C2,and EDNRA)regulating dose transcription was constructed by RNA sequencing.Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells(SMSCs) and their overexpression significantly increased the expression of SAA3.The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes. 展开更多
关键词 Boer goat CNV Muscle development SMSCs
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Poor maternal nutrition inhibits muscle development in ovine offspring 认领 引用 被引量：1
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作者 Sarah A Reed Joseline S Raja +2 位作者 Maria L Hoffman Steven A Zinn Kristen E Govoni 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2015年第1期79-89,共11页
Background： Maternal over and restricted nutrition has negative consequences on the muscle of offspring by reducing muscle fiber number and altering regulators of muscle growth. To determine if over and restricted ma... Background： Maternal over and restricted nutrition has negative consequences on the muscle of offspring by reducing muscle fiber number and altering regulators of muscle growth. To determine if over and restricted maternal nutrition affected muscle growth and gene fed 60%, 100% or 140% of National Research Council and protein expression in offspring, 36 pregnant ewes were requirements from d 31 ＋ 1,3 of gestation until parturition. Lambs from control-fed （CON）, restricted-fed （RES） or over-fed （OVER） ewes were necropsied within 1 d of birth （n = 18） or maintained on a control diet for 3 mo （n = ] 5）. Semitendinosus muscle was collected for immunohistochemistry, and protein and gene expression analysis. Results： Compared with CON, muscle fiber cross-sectional area （CSA） increased in RES （58%） and OVER （47%） lambs at 1 d of age （P〈 0.01）; however at 3 mo, CSA decreased 15% and 17% compared with CON, respectively （P 〈 0.01）. Compared with CON, muscle lipid content was increased in OVER （212.4%） and RES （92.5%） at d 1 （P 〈 0.0001）. Muscle lipid content was increased 36.1% in OVER and decreased 23.6% in RES compared with CON at 3 mo （P 〈 0.0001）. At d 1, myostatin mRNA abundance in whole muscle tended to be greater in OVER （P = 0.07） than CON. Follistatin mRNA abundance increased in OVER （P = 0.04） and tended to increase in RES （P = 0.06） compared with CON at d 1. However, there was no difference in myostatin or follistatin protein expression （P 〉 0.3）. Phosphorylated Akt （ser473） was increased in RES at 3 mo compared with CON （P = 0.006）. Conclusions： In conclusion, maternal over and restricted nutrient intake alters muscle lipid content and growth of offspring, possibly through altered gene and protein expression. 展开更多
关键词 Growth Maternal nutrition Muscle development Sheep
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Aflatoxin B1 decreased flesh flavor and inhibited muscle development in grass carp(Ctenopharyngodon idella) 认领 引用 被引量：7
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作者 Xiang Ning He Zhen Zhen Zeng +7 位作者 Wei Dan Jiang Pei Wu Yang Liu Sheng Yao Kuang Ling Tang Shu Wei Li Lin Feng Xiao Qiu Zhou 《Animal Nutrition》 SCIE CAS CSCD 2024年第3期27-38,共12页
In nature,aflatoxins,especially aflatoxin B1(AFB1),are the common mycotoxins,which cause serious health problems for humans and animals.This paper aimed to study the effects of AFB1 on flesh flavor and muscle developm... In nature,aflatoxins,especially aflatoxin B1(AFB1),are the common mycotoxins,which cause serious health problems for humans and animals.This paper aimed to study the effects of AFB1 on flesh flavor and muscle development of grass carp(Ctenopharyngodon idella)and its mechanism.There were 1440 individual fish in total,with 6 treatments and each treatment replicated 3 times.The 6 treatments were fed a control diet with different doses of AFB1(0.04,29.48,58.66,85.94,110.43 and 146.92μg/kg diet)for 60 d.AFB1 increased myofiber diameter,as well as decreased myofiber density of grass carp muscle(P<0.05).The contents of free amino acid decreased gradually(P<0.05)as dietary AFB1 increased in the muscle of grass carp.The levels of reactive oxygen species,malonaldehyde and protein carbonyl(PC)were increased(P<0.05)with the dietary AFB1 increased.The levels of antioxidant enzyme(glutathione peroxidase,glutathione,glutathione reductase,total antioxidant capacity,anti-superoxide anion,and anti-hydroxyl radical)were decreased(P<0.05)with the dietary AFB1 increased.In addition,dietary AFB1 decreased the content of collagen,and downregulated the mRNA and protein levels of transforming growth factor-β(TGF-β)/Smads signaling pathway in grass carp muscle(P<0.05).The mRNA and protein levels of myogenic regulatory factors were downregulated in grass carp muscle(P<0.05).Furthermore,the activities of matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)were increased(P<0.05),and the protein levels of phosphorylate-38 mitogen-activated protein kinase(p-p38MAPK),phosphorylate-c-Jun N-terminal kinase,urokinase-type plasminogen activator(uPA),MMP-2 and MMP-9 were upregulated(P<0.05),but collagenⅠ,lamininβ1 and fibronectin were downregulated(P<0.05)with the dietary AFB1 increased in the muscle of grass carp.Based on the results of this study,we can draw the following conclusion:dietary AFB1 might damage flesh flavor and inhibit the muscle development through MAPK/uPA/MMP/extracellular matrix(ECM)signaling pathway in grass carp.Moreover,the recommended safe limit of AFB1 in feed is no more than 26.77μg/kg diet according to the PC levels in grass carp muscle. 展开更多
关键词 Aflatoxin B1 Flesh flavor Muscle development Grass carp
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A new insight on alleviating the inhibitory effect of aflatoxin B1 on muscle development in grass carp(Ctenopharyngodon idella):The effect of 4-Methylesculetin in vivo and in vitro 认领 引用 被引量：2
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作者 Xiangning He Jiajia Zhang +7 位作者 Weidan Jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Hequn Shi Xiaoqiu Zhou Lin Feng 《Animal Nutrition》 SCIE CAS CSCD 2024年第4期339-354,共16页
Aflatoxin B1(AFB1), an important fungal toxin, exists mainly in plant feed ingredients and animals consuming feed contaminated with AFB1 will have reduced growth and impaired health condition mainly due to oxidative s... Aflatoxin B1(AFB1), an important fungal toxin, exists mainly in plant feed ingredients and animals consuming feed contaminated with AFB1 will have reduced growth and impaired health condition mainly due to oxidative stress and reduced immunity. Our previous study found that AFB1 caused oxidative damage and inhibited muscle development of zebrafish. 4-Methylesculetin(4-ME), a coumarin derivative, is now used in biochemistry and medicine widely because of its antioxidant function.Whether 4-ME could alleviate the inhibition of muscle development in grass carp induced by AFB1 has not been reported. In this experiment, 720 healthy grass carp(11.40 ± 0.01 g) were randomly divided into4 groups with 3 replicates of 60 fish each, including control group, AFB1 group(60 μg/kg diet AFB1), 4-ME group(10 mg/kg diet 4-ME), and AFB1+4-ME group(60 μg/kg diet AFB1 + 10 mg/kg 4-ME diet), for a60-d growth experiment. In vitro, we also set up 4 treatment groups for grass carp primary myoblast,including control group, AFB1 group(15 μmol/L AFB1), 4-ME group(0.5 μmol/L 4-ME) and AFB1+4-ME group(15 μmol/L AFB1+0.5 μmol/L 4-ME). The results showed that dietary AFB1 decreased growth performance of grass carp, damaged the ultrastructure and induced oxidative damage in grass carp muscle, and significantly decreased the m RNA and protein expression levels of myogenin(Myo G),myogenic differentiation(Myo D), myosin heavy chain(MYHC), as well as the protein expression levels of laminin β1, fibronectin and collagen I(P < 0.05), significantly activated the protein expression levels of urokinase-type plasminogen activator(u PA), matrix metalloproteinase-2(MMP-2), matrix metalloproteinase-9(MMP-9) and phosphorylate-38 mitogen-activated protein kinase(p38 MAPK) both in grass carp muscle and grass carp primary myoblast(P < 0.05). Supplementation of AFB1 with 4-ME significantly improved the growth performance inhibition and alleviated the muscle fiber development inhibition and extracellular matrix(ECM) degradation in grass carp induced by AFB1(P < 0.05). The present results revealed that supplementation of AFB1 contaminated feed with 4-ME reduced the inhibition of growth and muscle development by alleviating AFB1-induced ECM degradation in grass carp,which might be related to the p38 MAPK/u PA/MMP/ECM pathway. The results implied that 4-ME could be used as a valuable mycotoxin scavenger in animal feed. 展开更多
关键词 4-Methylesculetin Aflatoxin B1 Muscle development Grass carp
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The genetic regulation of skeletal muscle development:insights from chicken studies 认领 引用 被引量：2
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作者 Wen LUO Bahareldin A.ABDALLA +1 位作者 Qinghua NIE Xiquan ZHANG 《Frontiers of Agricultural Science and Engineering》 2017年第3期295-304,共10页
Skeletal muscle development is a complex multi-process trait regulated by various genetic factors.The chicken embryo is an ideal model system for studying skeletal muscle development. However, only a small proportion ... Skeletal muscle development is a complex multi-process trait regulated by various genetic factors.The chicken embryo is an ideal model system for studying skeletal muscle development. However, only a small proportion of the genetic factors affecting skeletal muscle development have been identified in chicken. The aim of this review is to summarize recent knowledge about the genetic factors involved in the regulation of skeletal muscle development in the chicken, such as gene polymorphisms, epigenetic modification, noncoding RNAs and transcription factors, which can influence skeletal muscle development at the genome, epigenome,transcriptome and proteome levels. Research on the regulation of skeletal muscle development in chicken is not yet comprehensive and most of the candidate genes and single nucleotide polymorphisms related to chicken muscle growth remain to be verified in experimental studies. In addition, the data derived from transcriptome sequencing and genome-wide association studies still require further investigation and analysis and comprehensive studies on the regulation of chicken skeletal muscle development will continue as a major research focus. 展开更多
关键词 chicken epigenetic modification miRNAs skeletal muscle development SNP transcription factor
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Proline‑driven metabolic reprogramming promotes skeletal muscle hypertrophy and oxidative myofiber specification in porcine offspring:a stage‑optimized maternal nutritional intervention 认领 引用
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作者 Jun Huang Kaidi Ma +6 位作者 Junyi Wu Shuangbo Huang Zihao Huang Yujiao Chen Shijian Zhou Hefeng Luo Chengquan Tan 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2446-2457,共12页
Background While maternal proline(Pro)supplementation has demonstrated efficacy in enhancing placental angiogenesis and farrowing efficiency in swine,its regulatory role in fetal skeletal muscle ontogeny remains undef... Background While maternal proline(Pro)supplementation has demonstrated efficacy in enhancing placental angiogenesis and farrowing efficiency in swine,its regulatory role in fetal skeletal muscle ontogeny remains undefined.This study systematically evaluated the temporal-specific impacts of dietary Pro supplementation during critical phases of fetal myogenesis(encompassing primary myofiber formation and secondary myofiber hyperplasia)on offspring muscle development.A total of 120 sows with similar farrowing schedules were assigned to three groups:CON(basal diet),ST-Pro(0.5%Pro supplementation during secondary myofiber formation period,from d 60 gestation to farrowing),LT-Pro(0.5%Pro supplementation spanning primary and secondary myofiber formation period:from d 20 gestation to farrowing).Results LT-Pro group significantly increased the longissimus dorsi(LD)muscle mass per unit body weight in newborn piglets compared to CON group(P<0.05),while no such effect was observed in the ST-Pro group.Metabolomic profiling revealed elevated Pro,lysine,and tryptophan levels in the LD muscle of LT-Pro group piglets,accompanied by reduced branched-chain amino acids(BCAAs;leucine,isoleucine,and valine)in both serum and muscle(P<0.05).Histological analysis demonstrated a 45.74%increase in myofiber cross-sectional area in the LT-Pro group(P<0.05).At the molecular level,LT-Pro group piglets exhibited upregulated mRNA expression levels of myogenic regulatory genes(MYOD1,MYF6)and the cell cycle accelerator CCND1(P<0.05),coupled with activation of the STAT3 signaling pathway(phosphorylated STAT3 protein increased by 2.53-fold,P<0.01).Furthermore,Pro supplementation enhanced oxidative metabolism,evidenced by elevated mitochondrial biogenesis markers(the mRNA expression levels of PPARGC1A,OPA1,and SQSTM1)and a 61.58%increase in succinate dehydrogenase activity(P<0.05).Notably,LT-Pro group piglets showed a selective shift toward slow-twitch oxidative fibers,with both MyHC1 mRNA and protein expression levels significantly upregulated(P<0.05),while the mRNA expression levels of MyHCIIb showed no significant change.Conclusions This study identified the primary fiber formation period as a critical window.Supplementation with Pro during G20–114 reprogrammed offspring skeletal muscle development through STAT3-CCND1-mediated myoblast proliferation,enhanced mitochondrial bioenergetics,and oxidative fiber specification.However,no such effects were observed during G60–114.These findings propose maternal Pro intervention as a novel strategy to enhance muscle yield and metabolic efficiency in swine production,with potential applications for improving meat quality traits linked to oxidative muscle phenotypes. 展开更多
关键词 Mitochondrial function Oxidative muscle fibers Proline Skeletal muscle development STAT3 signaling pathway
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Circtefb Regulates Myocytes Development by Sponging Pol-miR-138 in Japanese Flounder(Paralichthys olivaceus) 认领 引用
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作者 LIU Min WANG Hao +3 位作者 TANG Hengtai LIU Binghua WEN Haishen HE Feng 《Journal of Ocean University of China》 SCIE CAS CSCD 2025年第5期1364-1372,I0665,I0666,共9页
Circular RNAs(circRNAs)are a type of endogenous competitive RNA that can perform various functions and regulate a number of biological activities in organisms.Many studies have shown that circRNAs can modulate skeleta... Circular RNAs(circRNAs)are a type of endogenous competitive RNA that can perform various functions and regulate a number of biological activities in organisms.Many studies have shown that circRNAs can modulate skeletal muscle development in animals.However,the effects of circRNAs on the skeletal muscle development of Japanese flounder and its regulatory mechanisms remain unclear.We screened circRNA_2111 from previously obtained transcriptomic data related to Japanese flounder skeletal mus-cle development.Derived from the tefb gene,it has been designated as circtefb.Circtefb was formed by the reverse splicing of two exons of tefb.Circtefb was more stable than its corresponding linear RNA.There was a significant difference in the expression of circtefb at different stages P1(90 d),P2(12 months)and P3(24 months)of Japanese flounder(p<0.05).Over-expression of circtefb in Japanese flounder myocytes resulted in a significant upregulation of proliferation marker genes ccnd1,ccnd2a and pcna(p<0.05),suggesting that it promoted proliferation of Japanese flounder myocytes.The expressions of ccnd1,ccnd2a,and pcna were signifi-cantly decreased after the transfection of pol-miR-138 into Japanese flounder myocytes(p<0.05).In addition,circtefb can suppress pol-miR-138 function by binding to it,which was detected by dual-Luciferase reporter assay and co-transfection of circtefb and pol-miR-138,thereby promoting the proliferation of Japanese flounder myocytes.The results indicated the function of circRNAs in Japanese flounder muscle development,,which is also helpful to understand the growth and development of other fish species. 展开更多
关键词 circtefb skeletal muscle development Japanese flounder
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Function and regulation of muscle stem cells in skeletal muscle development and regeneration:a narrative review 认领 引用
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作者 Han Zhu Xin Lin Yarui Diao 《Journal of Bio-X Research》 2021年第3期89-96,共8页
Skeletal muscle plays an essential role in generating the mechanical force necessary to support the movement of our body and daily exercise. Compared with cardiac and smooth muscle, in mammals, skeletal muscle exhibit... Skeletal muscle plays an essential role in generating the mechanical force necessary to support the movement of our body and daily exercise. Compared with cardiac and smooth muscle, in mammals, skeletal muscle exhibits remarkable regenerative capacity in response to damage. Muscle stem cells, also known as satellite cells, directly contribute to regeneration. Here, we review primary and secondary myogenesis processes with a focus on muscle stem cells, as well as the function and regulation of muscle stem cells in adult muscle regeneration in mammals. 展开更多
关键词 muscle stem cell satellite cell skeletal muscle development skeletal muscle regeneration stem cell niche
Integrative single-cell RNA-seq and ATAC-seq profiling identifies NTN5+LSAMP+myoblasts as mediators of impaired embryonic myogenesis 认领 引用
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作者 Rong Xu Xiao-Yu Wang +7 位作者 Zhi-Ting Feng Shu-Fang Cai Zi-Yun Liang Li-Ping Pan Qing-Shuang Zhou Xiao-Hong Liu Yao-Sheng Chen De-Lin Mo 《Zoological Research》 SCIE CSCD 2026年第2期561-576,共16页
Prenatal myogenesis establishes the cellular and regulatory framework that determines postnatal muscle mass and contractile performance,and represents a critical period for elucidating mechanisms underlying developmen... Prenatal myogenesis establishes the cellular and regulatory framework that determines postnatal muscle mass and contractile performance,and represents a critical period for elucidating mechanisms underlying developmental myopathies and impaired fetal growth.In this study,integrated single-cell transcriptomic and chromatin accessibility profiling was applied to compare the regulatory landscapes between growth-restricted and normal porcine embryonic somites.This analysis uncovered a distinct pathogenic myoblast population characterized by co-expression of netrin-5(NTN5)and limbic system-associated membrane protein(LSAMP),markedly enriched in growth-restricted embryos.These NTN5+LSAMP+myoblasts exhibited a cell-autonomous differentiation blockade accompanied by coordinated epigenetic and metabolic dysregulation.Chromatin landscapes revealed sustained accessibility at the cytoskeletal regulator tropomyosin-3(TPM3),suppressed accessibility at the muscle-specific actin depolymerization factor cofilin-2(CFL2),and transcriptional programs consistent with impaired glycolytic flux.Perturbation of the Hippo/TGF-βsignaling pathway,together with disrupted ligand–receptor interactions,exacerbated the differentiation arrest.Reconstruction of a multi-omics regulatory network delineated the mechanisms underlying aberrant embryonic myogenesis,identifying NTN5 and LSAMP as key regulators of porcine skeletal muscle development.Collectively,these findings define mechanistic determinants of impaired fetal myogenesis and provide candidate targets for therapeutic intervention in growth restriction as well as molecular strategies for optimizing muscle accretion in livestock. 展开更多
关键词 Pig Differentiation arrest Skeletal muscle development NTN5 LSAMP
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Sequence-based genome-wide association study reveals genetic and metabolic mechanisms underlying feed efficiency-related traits in beef cattle 认领 引用
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作者 Leonardo M.Arikawa Lucio F.M.Mota +11 位作者 Larissa F.S.Fonseca Gerardo A.Fernandes Júnior Bruna M.Salatta Gabriela B.Frezarim Patricia I.Schmidt Sindy L.C.Nasner Julia P.S.Valente Amalia M.Pelaez Roberta C.Canesin Josineudson A.Ⅱ.Ⅴ.Silva Maria Eugênia Z.Mercadante Lucia G.Albuquerque 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第3期1264-1283,共20页
Background Efficiency is characterized by maximum productivity with lower inputs and minimal waste,resulting in greater output with the same or even fewer resources.In livestock,more efficient animals in converting fo... Background Efficiency is characterized by maximum productivity with lower inputs and minimal waste,resulting in greater output with the same or even fewer resources.In livestock,more efficient animals in converting food into protein may improve the economic efficiency of production systems,as feed costs represent a significant expense in beef production.Thus,the present study aimed to use imputed whole-genome sequencing(WGS)data to perform a genome-wide association study(GWAS)in order to identify genomic regions and potential candidate genes involved in the biological processes and metabolic pathways associated with feed efficiency-related traits(RFI:residual feed intake,DMI:dry matter intake,FE:feed efficiency,FC:feed conversion,and RWG:residual weight gain)in Nellore cattle.Results The GWAS identified significant SNPs associated with feed efficiency traits in Nellore cattle.A total of 42 SNPs were detected for RFI,10 for DMI,99 for FC,15 for FE,and 3 for RWG,distributed in different autosomes.Annotation analysis identified several candidate genes,and the prioritization highlighted 21,9,68,23,and 8 key genes for RFI,DMI,FC,FE,and RWG,respectively.The prioritized candidate genes are involved in muscle development,lipid metabolism,response to oxidative stress,nutrient metabolism,neurotransmission,and oxidative phosphorylation.Additionally,enrichment analysis indicated that these genes act in several signaling pathways related to signal transduction,the nervous system,the endocrine system,energy metabolism,the digestive system,and nutrient metabolism.Conclusion The use of imputed WGS data in GWAS analyses enabled the broad identification of regions and candidate genes throughout the genome that regulate expression of feed efficiency-related traits in Nellore cattle.Our results provide new perspectives into the molecular mechanisms underlying feed efficiency in Nellore cattle,offering a genetic basis to guide the breeding of efficient animals,thereby optimizing resource utilization and the profitability of production systems. 展开更多
关键词 Bos indicus Energy metabolism Imputed WGS Muscle development Oxidative phosphorylation Residual feed intake
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Effect of Botubinum toxin type A injection into the masseter muscle on mandibular development in rats 认领 引用
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作者 黄进军 《外科研究与新技术（中英文）》 2011年第2期132-132,共1页
Objective To investigate the effect of Botulinum toxin type A(Botox)injection into the masseter muscle on mandibular development in rats.Methods 1228-day-old Wistar rats were divided into two groups as Botox group(n=6... Objective To investigate the effect of Botulinum toxin type A(Botox)injection into the masseter muscle on mandibular development in rats.Methods 1228-day-old Wistar rats were divided into two groups as Botox group(n=6)and control group(n=6) 展开更多
关键词 Effect of Botubinum toxin type A injection into the masseter muscle on mandibular development in rats type
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Transcriptomic and epigenomic landscapes of muscle growth during the postnatal period of broilers 认领 引用 被引量：4
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作者 Shuang Gu Qiang Huang +3 位作者 Yuchen Jie Congjiao Sun Chaoliang Wen Ning Yang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第5期1851-1865,共15页
Background Broilers stand out as one of the fastest-growing livestock globally,making a substantial contribution to animal meat production.However,the molecular and epigenetic mechanisms underlying the rapid growth an... Background Broilers stand out as one of the fastest-growing livestock globally,making a substantial contribution to animal meat production.However,the molecular and epigenetic mechanisms underlying the rapid growth and development of broiler chickens are still unclear.This study aims to explore muscle development patterns and regulatory networks during the postnatal rapid growth phase of fast-growing broilers.We measured the growth performance of Cornish(CC)and White Plymouth Rock(RR)over a 42-d period.Pectoral muscle samples from both CC and RR were randomly collected at day 21 after hatching(D21)and D42 for RNA-seq and ATAC-seq library construction.Results The consistent increase in body weight and pectoral muscle weight across both breeds was observed as they matured,with CC outpacing RR in terms of weight at each stage of development.Differential expression analysis identified 398 and 1,129 genes in the two dimensions of breeds and ages,respectively.A total of 75,149 ATAC-seq peaks were annotated in promoter,exon,intron and intergenic regions,with a higher number of peaks in the promoter and intronic regions.The age-biased genes and breed-biased genes of RNA-seq were combined with the ATAC-seq data for subsequent analysis.The results spotlighted the upregulation of ACTC1 and FDPS at D21,which were primarily associated with muscle structure development by gene cluster enrichment.Additionally,a noteworthy upregulation of MUSTN1,FOS and TGFB3 was spotted in broiler chickens at D42,which were involved in cell differentiation and muscle regeneration after injury,suggesting a regulatory role of muscle growth and repair.Conclusions This work provided a regulatory network of postnatal broiler chickens and revealed ACTC1 and MUSTN1 as the key responsible for muscle development and regeneration.Our findings highlight that rapid growth in broiler chickens triggers ongoing muscle damage and subsequent regeneration.These findings provide a foundation for future research to investigate the functional aspects of muscle development. 展开更多
关键词 ATAC-seq Broiler Pectoral muscle development Rapid growth RNA-seq
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Tumor necrosis factor α deficiency promotes myogenesis and muscle regeneration 认领 引用 被引量：1
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作者 Yu Fu Jing-Ru Nie +4 位作者 Peng Shang Bo Zhang Da-Wei Yan Xin Hao Hao Zhang 《Zoological Research》 SCIE CSCD 2024年第4期951-960,共10页
Tumor necrosis factorα(TNFα)exhibits diverse biological functions;however,its regulatory roles in myogenesis are not fully understood.In the present study,we explored the function of TNFαin myoblast proliferation,d... Tumor necrosis factorα(TNFα)exhibits diverse biological functions;however,its regulatory roles in myogenesis are not fully understood.In the present study,we explored the function of TNFαin myoblast proliferation,differentiation,migration,and myotube fusion in primary myoblasts and C2C12 cells.To this end,we constructed TNFαmuscle-conditional knockout(TNFα-CKO)mice and compared them with flox mice to assess the effects of TNFαknockout on skeletal muscles.Results indicated that TNFα-CKO mice displayed phenotypes such as accelerated muscle development,enhanced regenerative capacity,and improved exercise endurance compared to flox mice,with no significant differences observed in major visceral organs or skeletal structure.Using label-free proteomic analysis,we found that TNFα-CKO altered the distribution of several muscle development-related proteins,such as Hira,Casz1,Casp7,Arhgap10,Gas1,Diaph1,Map3k20,Cfl2,and Igf2,in the nucleus and cytoplasm.Gene set enrichment analysis(GSEA)further revealed that TNFαdeficiency resulted in positive enrichment in oxidative phosphorylation and MyoD targets and negative enrichment in JAK-STAT signaling.These findings suggest that TNFα-CKO positively regulates muscle growth and development,possibly via these newly identified targets and pathways. 展开更多
关键词 TNFα Muscle-conditional knockout Myogenesis Regeneration Muscle development
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BTG1 as a New Candidate Gene for Muscle Growth in Pigs: Cloning,Expression and Association Analysis 认领 引用 被引量：2
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作者 Zheng Feng He Shen +5 位作者 Zhiqiang Du Mengjin Zhu Bin Fan M. F. Rothschild Shuhong Zhao Changchun Li 《Journal of Animal Science and Biotechnology》 CAS 2011年第3期121-130,共10页
BTG1 （B-cell Translocation Gene 1） , a member of the BTG / TOB （Transducer of ErbB-2） family of anti-proliferation factors,has been proven to have an unfavorable effect on muscle fiber growth in several species. T... BTG1 （B-cell Translocation Gene 1） , a member of the BTG / TOB （Transducer of ErbB-2） family of anti-proliferation factors,has been proven to have an unfavorable effect on muscle fiber growth in several species. The porcine BTG1 gene was cloned and its 5＇ flanking promoter region sequence, and characterized the expression patterns in different tissues of adult pigs and in fetal skeletal muscle at different developmental stages in two breeds. The tissue distribution pattern analyses revealed that the mRNA of porcine BTG1 was ubiquitously expressed in the six tissues of both Landrace and Tongcheng pigs. Real-time quantitative reverse transcriptase-PCR results showed that BTG1 mRNA expression levels were significantly different among the three fetal ages in Tongcheng pigs,while no significant differences were found among the three ages in Landrace pigs. Furthermore,the expression of BTG1 in Landrace pigs was significantly lower than in Tongcheng pigs at all three ages. The temporal expression profiles of the BTG1 gene in mouse myoblast C 2 C 12 cells were shown to be consistent with those of the myogenin gene. A single nucleotide polymorphism （SNP） ,g. 281C 〉 T,was identified in the 3＇UTR and allele frequencies were detected in seven pig breed populations. Significant associations were found between the g. 281C 〉 T polymorphism and growth and meat quality traits. Our results indicate that the porcine BTG1 gene could play a potential role in markerassisted selection and as such may be a gene of economic importance. 展开更多
关键词 BTG1 C 2 C 12 cells gene expression muscle development pigs
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Targeted myostatin loss-of-function mutation increases type Ⅱ muscle fibers in Meishan pigs 认领 引用 被引量：2
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作者 QIAN Li-li XIE Jing-yi +5 位作者 GAO Ting CAI Chun-bo JIANG Sheng-wang BI Han-fang XIE Shan-shan CUI Wen-tao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第1期188-198,共11页
Myostatin(MSTN) is a negative regulator of skeletal muscle growth and development. The skeletal muscle in MSTN-/-mice is significantly hypertrophied, with muscle fiber type II increasing significantly while muscle ... Myostatin(MSTN) is a negative regulator of skeletal muscle growth and development. The skeletal muscle in MSTN-/-mice is significantly hypertrophied, with muscle fiber type II increasing significantly while muscle fiber type I decreasing.However, it is still not clear how the skeletal muscle types change in MSTN-/-pigs, and how the mechanism for MSTN regulates fiber types, especially in large animals like pigs. This study conducted a comprehensive analysis of the composition of skeletal muscle fibers in MSTN-/-pigs produced in our laboratory. It was observed that, compared with wild-type(WT) pigs, both the total mass of skeletal muscle and type IIb muscle fibers increased significantly(P<0.01),while the type I and type IIa muscle fibers decreased significantly(P<0.01), in MSTN-/-Meishan pigs. In addition, to explore the influence of MSTN on muscle fiber type and its regulation mechanism in the embryonic stage, this study selected a few genes(Myf5, Mef2 d, MyoD and Six1) associated with muscle fiber type and validated their expression by quantitative RT-PCR. Herein, it was found that Myh7, Myh2, Myh4 and Myh1 can be detected in the skeletal muscle of pigs at 65 days of gestation(dg). Compared with WT pigs, in MSTN-/-Meishan pigs, Myh7 decreased significantly(P<0.01), while Myh4(P<0.001) and Myh1(P<0.05) increased significantly. Meanwhile, the increased expression of Myf5(P<0.05), Mef2 d(P<0.01) and Six1(P<0.05) in MSTN-/-Meishan pigs suggested that MSTN should regulate the directional development of muscle fiber types in the early stage of embryonic development. Thus, at the embryonic stage, the type II muscle fibers began to increase in MSTN-/-pigs. These results can provide valuable information not only for pig meat quality improvement, but also for the study of human skeletal muscle development and disease treatment. 展开更多
关键词 MSTN Meishan pigs muscle fiber type muscle fiber development
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Integration of genome-wide association study and selection signatures reveals genetic determinants for skeletal muscle production traits in an F2 chicken population 认领 引用 被引量：2
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作者 LI Yu-dong BAI Xue +8 位作者 LIU Xin WANG Wei-jia LI Zi-wei WANG Ning XIAO Fan GAO Hai-he GUO Huai-shun LI Hui WANG Shou-zhi 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第7期2065-2075,共11页
Improving the production of broiler chicken meat has been a goal of broiler breeding programs worldwide for many years.However,the genetic architectures of skeletal muscle production traits in chickens have not yet be... Improving the production of broiler chicken meat has been a goal of broiler breeding programs worldwide for many years.However,the genetic architectures of skeletal muscle production traits in chickens have not yet been fully elucidated.In the present study,a total of 519 F2birds,derived from a cross of Arbor Acres broiler and Baier layer,were re-sequenced(26 F0individuals were re-sequenced at a 10-fold depth;519 F2individuals were re-sequenced at a 3-fold depth)and the coupling of genome-wide association study(GWAS)and selection signatures(FST(fixation index)andθπ(nucleotide diversity))was carried out to pinpoint the associated loci and genes that contribute to pectoral muscle weight(PMW)and thigh muscle weight(TMW).A total of 7890258 single nucleotide polymorphisms(SNPs)remained to be analyzed after quality control and imputation.The integration of GWAS and selection signature analyses revealed that genetic determinants responsible for skeletal muscle production traits were mainly localized on chromosomes 1(168.95–172.43 Mb)and 4(74.37–75.23 Mb).A total of 17 positional candidate genes(PCGs)(LRCH1,CDADC1,CAB39 L,LOC112531568,LOC112531569,FAM124 A,FOXO1,NBEA,GPALPP1,RUBCNL,ARL11,KPNA3,LHFP,GBA3,LOC112532426,KCNIP4,and SLIT2)were identified in these regions.In particular,KPNA3 and FOXO1 were the most promising candidates for meat production in chickens.These findings will help enhance our understanding of the genetic architecture of chicken muscle production traits,and the significant SNPs identified could be promising candidates for integration into practical breeding programs such as genome-wide selection(GS)to improve the meat yield of chickens. 展开更多
关键词 chicken muscle growth and development GWAS selection signature
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