The objective of this study was to investigate the effects of total dietary fiber level on nutrient digestibility and the relationship between apparent total tract digestibility of total dietary fiber, and soluble die...The objective of this study was to investigate the effects of total dietary fiber level on nutrient digestibility and the relationship between apparent total tract digestibility of total dietary fiber, and soluble dietary fiber, insoluble dietary fiber and available energy. Sugar beet pulp was as the only fiber source. The experiment was designed as a 6 x 6 Latin square with an adaptation period of 7 d followed by a 5-d total collection of feces and urine. Feed intake tended to decrease (P =0.] O) as total dietary fiber level increased. The apparent total tract digestibility of dry matter, crude protein and gross energy decreased (P 〈0.0]) when total dietary fiber increased but the digestibility of soluble dietary fiber and insoluble dietary fiber increased (P 〈0.01). The digestible energy and metabolizable energy content of diets decreased (P 〈0.01) as the total dietary fiber increased.展开更多
Background:This study aimed to investigate the hydration properties of different-source fibrous materials by com-paring their water-binding capacity(WBC),water swelling capacity(WSC),viscosity,and in vivo effects of s...Background:This study aimed to investigate the hydration properties of different-source fibrous materials by com-paring their water-binding capacity(WBC),water swelling capacity(WSC),viscosity,and in vivo effects of selected samples on growth performance,nutrient digestibility,diarrhea,and intestinal health in weaned piglets.Methods:A total of 13 commercially available fibrous materials were first compared in chemical composition and in vitro hydration property.Subsequently,40 weaned piglets were randomized to five experimental dietary groups(8 piglets per group):control diet(a basal diet without dietary fiber,CON),basal diet supplemented with 5%microcrys-talline cellulose(MCC),5%wheat bran(WB),5%Moringa oleifera leaf powder(MOLP),or 5%sugar beet pulp(SBP),fol-lowed by analyzing their growth performance and diarrhea rate in a 28-d experiment.After the feeding experiment,anaesthetized piglets were killed,and their intestinal and colon content or plasma samples were analyzed in nutrient digestibility,intestinal morphology,intestinal barrier,short-chain fatty acids(SCFAs),and bacterial population.Results:In vitro studies showed low hydration properties for WB and MCC,while medium hydration properties for MOLP and SBP.In vivo studies indicated that compared with medium hydration property groups,low hydration prop-erty groups showed(1)exacerbated diarrhea,impaired intestinal health,and reduced apparent fecal digestibility of dry matter,gross energy,acid detergent fiber,and neutral detergent fiber;(2)decreased SCFAs concentration and rela-tive levels of Lactobacillus and Bifidobacterium,but increased levels of Escherichia coli and Brachyspira hyodysenteriae in colon contents.Additionally,SBP showed optimal performance in reducing diarrhea and increasing SCFAs produc-tion.Correlation analysis revealed a positive correlation of fiber hydration properties with in vitro SCFAs production,and diarrhea index and nutrient digestibility were negatively and positively correlated with SCFAs levels in the colon contents of weaned piglets,respectively.Conclusions:Different-source dietary fibers varied in their hydration properties and impacts on diarrhea,microbial composition and SCFAs production in weaned piglets.WB and MCC could exacerbate diarrhea and impair nutrient digestibility,probably because their low hydration properties were detrimental to gut microbial homeostasis and fermentation.Our findings provide new ideas for rational use of fiber resources in weaned piglets.展开更多
Extruded soybean meal (ESBM) was evaluated as a protein source for partial replacement of fish meal (FM) in diets of juvenile Litopenaeus vannarnei. In the control diet (Diet 1), FM protein was replaced with inc...Extruded soybean meal (ESBM) was evaluated as a protein source for partial replacement of fish meal (FM) in diets of juvenile Litopenaeus vannarnei. In the control diet (Diet 1), FM protein was replaced with increasing dietary levels of ESBM (4.28%, 8.40%, 12.62%, 16.82%, and 25.26%) at 10%, 20%, 30%, 40%, and 60% levels (Diets 2 to 6, respectively). An eight-week feeding trial was conducted on 720 juvenile shrimp (0.67 g ± 0.01 g mean initial Weight), and nutrient digestibility of the six diets was determined. ESBM could replace 20% of FM without causing a significant reduction in growth of shrimp, but other dietary treatments strongly affected whole body composition. Crude protein content of the whole body fed Diet 6 was significantly lower than that fed Diet 2 (P〈0.05), while crude lipid content of the whole body fed Diet 5 or 6 was significantly higher than that fed Diet 2 (P〈0,05). Protein digestibilities of Diets 5 and 6 were significantly lower than that of Diet 1 (P〈0.05). Digestibility of lipids ranged from 96.97% in Diet 6 to 98.34% in Diet 3, whereas dry matter digestibility decreased with increasing replacement level. This study indicates that 20% FM replacement with ESBM in the basic diet containing 40% protein and 30% FM is optimal for juvenile L. vannamei.展开更多
A total of 480 nursery pigs (Duroc x Landrace x Large White) were utilized in two experiments conducted to determine the effects of different ratios of standardized ileal digestible lysine (SID- Lys) to metaboliza...A total of 480 nursery pigs (Duroc x Landrace x Large White) were utilized in two experiments conducted to determine the effects of different ratios of standardized ileal digestible lysine (SID- Lys) to metabolizable energy (ME) ratio on the performance, nutrient digestibility, plasma urea nitrogen (PUN) ,and plasma free amino acids of 10 to 28 kg pigs. In Exp. 1, 192 pigs ( 10.58 kg) were assigned to one of four treatments. The treatments consisted of diets with a ME content of 3.2, 3.25, 3.3, or 3.35 Mcal/kg with a constant SID-Lys:ME ratio of 3.7 g/Mcal. The experiment lasted 28 days. Pigs which were fed the diets containing 3.3 and 3.35 Mcal/kg ME had lower feed intakes ( P 〈 0.05)than those fed 3.2 Mcal/kg. Feed efficiency was linearly improved with increasing dietary ME ( P 〈0.05). Increasing the dietary ME level also increased (P 〈0.05 ) dry matter and energy digestibility. Therefore,3.3 Mcal/kg ME was selected for Exp. 2 in which 288 pigs ( 10.60 kg) were assigned to one of six treatments. Treatments consisted of SID-Lys: ME ratios of 3.1,3.3,3.5,3.7,3.9, or 4.1 g/Mcal with all diets providing 3.3 Mcal of ME/kg. Weight gain and feed efficiency were increased (P 〈0.05 ) as the SID-Lys:ME ratio in diet increased. Based on a straight broken-line model ,the estimated SID-Lys:ME ratio to maximize weight gain was 3.74.展开更多
The experiment was conducted to study the effects of low protein diets on nutrient digestibility and excretion of finishing pigs. For the 30-d trial,fortytwo duroc × landrace × yorkshire( DLY) finishing pigs...The experiment was conducted to study the effects of low protein diets on nutrient digestibility and excretion of finishing pigs. For the 30-d trial,fortytwo duroc × landrace × yorkshire( DLY) finishing pigs with the initial weight of( 91. 13 ± 1. 64) kg were randomly divided into two groups,with three replicates in each group. The pigs in the two groups were fed with different crude protein( CP) levels( control groups: 15. 49% and low protein groups: 12. 59%). The results showed that:( 1) The digestibility of dry matter and crude fat( CF) in low protein groups were increased significantly( P 0. 05). The results showed that 2. 9% reduction of the CP in diets could improve nutrient digestibility and reduce the excretion of N,but reduced the digestibility of Arg.展开更多
This experiment was conducted to determine the effects of a commercial enzyme mixture providing xylanase and protease activities on the digestibility of dietary nutrients when added to diets based on peas and wheat an...This experiment was conducted to determine the effects of a commercial enzyme mixture providing xylanase and protease activities on the digestibility of dietary nutrients when added to diets based on peas and wheat and fed to 9. 5 to 15 kg pigs. Pigs were weaned at 18 to 21 days of age and fitted with a simple T-cannula at the distal ileum,6 to 7 days after weaning. After 5 to 7 days for adaptation to their diets, ileal digesta, and feces were collected. Enzyme supplementation had no effect on the apparent ileal or fecal digestibility of any dietary nutrient. There was a numerical improvement ( P 〉 0.05 ) in the ileal digestibility of fiber. In conclusion, xylanase and protease appeared to be ineffective in improving nutrient digestibility when supplemented in diets based on peas and wheat fed to weaned pigs.展开更多
The study aimed at evaluating the effect of supplementing Saccharomyces cerevisiae into low quality local-based feeds on performance and nutrient digestibility of late starter to grower stage local pigs. There were to...The study aimed at evaluating the effect of supplementing Saccharomyces cerevisiae into low quality local-based feeds on performance and nutrient digestibility of late starter to grower stage local pigs. There were total 16 late starter local pigs fed with four treatment feeds based on block design with four pigs in each treatment. The four treatment feeds offered consisted of: commercial starter feeds Charoen Pokphand 552 (To), basal feeds + yeast 2% of daily feeds requirement (T0, basal feeds + yeast 4% of daily feeds requirement (T2) and basal feeds + yeast 6% of daily feeds requirement (T3). Feed intake, daily weight gain, feed conversion efficiency, protein and crude fiber digestibility were evaluated in the study. The findings showed that supplementing S. cerevisiae into low quality local-based diet improved 0.9%-2.7% feeds' crude protein, 10%-19% feeds intake, 1%-6% daily weight gain, 3%-4% crude protein digestibility and 4%-5% crude fiber digestibility, but reduced feeds conversion efficiency by 0.3-0.4. The conclusion drawn is that supplementing S. cerevisiae (yeast) up to 6% improved performances of starter local pigs fed low quality feeds and performed the similar result with feeding commercial starter feeds Charoen Pokphand 552. Further research by widening the range and increasing the level of yeast supplementation could be done.展开更多
One hundred and twenty day-old Anak 2,000 broilers were used to test the effect of replacing maize with cornflakes waste meal (CFWM) on their performance and apparent nutrient digestibility in a complete randomized ...One hundred and twenty day-old Anak 2,000 broilers were used to test the effect of replacing maize with cornflakes waste meal (CFWM) on their performance and apparent nutrient digestibility in a complete randomized design. Five dietary, treatments ( l, 2, 3, 4 and 5) were lbnnulated and CFWM was included in the diet in place of maize at concentrations of 0, 25, 50, 75 and 100% respectively. The results revealed that dietary, inclusion of CFWM from 25%-75% significantly (P 〈 0.05) increased average live weight and weight gain, bnt reduced the teed intake and improved the feed to gain ratio of birds compared to those on the control diet (0% CFWM). Besides, apparent digestible crude fibre, ether extract and ash of broilers on the control diet were lower (P 〈 0.05) thm~ those on CFWM based diets. The overall results show that CFWM can successfully replace maize tip to 75% levels as all energ) source with significant improvement in growth rate and nutrient retention of broiler starters.展开更多
This study was conducted to investigate the effect of dietary protease supplementation on the growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression in weaned piglets....This study was conducted to investigate the effect of dietary protease supplementation on the growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression in weaned piglets. A total of 300 weaned piglets(21 days of age Duroc x Large White x Landrace; initial BW = 6.27 ± 0.45 kg) were randomly divided into 5 groups. The 5 diets were: 1) positive control diet(PC), 2) negative control diet(NC), and 3) protease supplementations, which were 100, 200, and 300 mg per kg NC diet. Results indicated that final BW, ADG, ADFI, crude protein digestibility, enzyme activities of stomach pepsin, pancreatic amylase and trypsin, plasma total protein, and intestinal villus height were higher for the PC diet and the supplementations of 200 and 300 mg protease per kg NC diet than for the NC diet(P < 0.05). Supplementations of 200 and 300 mg protease per kg NC diet significantly increased the ratio of villus height to crypt depth(VH:CD) of duodenum, jejunum and ileum compared with NC diet(P < 0.05). Feed to gain ratio, diarrhea index, blood urea nitrogen, and diamine oxidase were lower for the PC diet and supplementations of 200 and 300 mg protease per kg NC diet than for the NC diet(P < 0.05). Piglets fed the PC diet had a higher peptide transporter 1(PepTl) mRNA abundance in duodenum than piglets fed the NC diet(P < 0.05), and supplementations of 100, 200 and 300 mg protease per kg NC diet increased the PepTl mRNA abundance in duodenum(P < 0.05) comparing with the NC diet. Piglets fed the PC diet had a higher b0,+AT mRNA abundance in jejunum than piglets fed the NC diet(P < 0.05), and supplementations of 200 and 300 mg protease per kg NC diet increased the b0,+AT mRNA abundance in jejunum and ileum comparing with the NC diet(P < 0.05). In summary, dietary protease supplementation increases growth performance in weaned piglets, which may contribute to the improvement of intestinal development, protein digestibility, nutrient transport efficiency, and health status of piglets when fed low digestible protein sources.展开更多
To explore the effects of fermented rapeseed meal(FRSM)on growth performance and intestinal health,a total of 30 growing pigs were randomly allotted to three treatments consisting of corn-soybean meal diet(CSD),rapese...To explore the effects of fermented rapeseed meal(FRSM)on growth performance and intestinal health,a total of 30 growing pigs were randomly allotted to three treatments consisting of corn-soybean meal diet(CSD),rapeseed meal diet(RSD),and fermented rapeseed meal diet(FRSD).Results showed that compared with RSD,FRSD feeding increased the average daily gain and final body weight in pigs(P<0.01).Compared with RSD feeding,FRSD feeding elevated the apparent digestibility of crude protein,acid detergent fiber,and ether extract in pigs(P<0.01).Moreover,the FRSD group exhibited greater apparent ileal digestibility of His,Thr,Lys,and Ser than the RSD group(P<0.01).The digestible energy,metabolic energy,and nitrogen utilization were higher in the FRSD and CSD groups than in the RSD group(P<0.01).As compared to the RSD,FRSD feeding decreased the serum concentration of leptin but significantly increased the concentrations of immunoglobulin(Ig)A,IgG,ghrelin,and enzyme activities of amylase,lipase,and trypsin in the pancreas(P<0.05).Interestingly,the villus height,the ratio of villus height to crypt depth,and the activities of brush border enzymes(e.g.,maltase and sucrase)in the small intestine were higher in the CSD and FRSD groups than in the RSD group(P<0.05).As compared to the RSD,the FRSD feeding not only increased the expression level of the occludin in the small intestinal epithelium(P<0.05)but also elevated the expression levels of claudin-1,MUC1,and PepT1 genes in the duodenum,and elevated the expression levels of SGLT1 and CAT1 genes in the jejunum(P<0.05).Importantly,FRSD feeding significantly decreased the abundance of Escherichia coli,but increased the abundance of Lactobacillus and the content of butyrate in the cecum and colon(P<0.05).These results indicated that compared with rapeseed meal,fermented rapeseed meal exhibited a positive effect on improving the growth performance and intestinal health in growing pigs,and the results may also help develop novel protein sources for animal nutrition and the feed industry.展开更多
This study was conducted to evaluate the prebiotic effects of dietary xylooligosaccharide(XOS)sup-plementation on performance,nutrient digestibility,intestinal morphology,and gut microbiota in laying hens.In a 12-wk e...This study was conducted to evaluate the prebiotic effects of dietary xylooligosaccharide(XOS)sup-plementation on performance,nutrient digestibility,intestinal morphology,and gut microbiota in laying hens.In a 12-wk experiment,a total of 288 Hy-Line Brown layers at 50 wk of age were randomly assigned into 3 dietary treatments supplemented with XOS at 0,200 or 400 mg/kg.Each treatment had 8 replicates with 12 birds each.Hens fed XOS diets showed a lower feed-to-egg ratio during wk 7 to 12 and a higher egg yolk color value in wk 12 compared with those fed the control diet(P<0.05).Dietary XOS supplementation improved the apparent total tract digestibility of gross energy and nitrogen at the end of the 12th wk(P<0.05).In addition,a higher villus height-to-crypt depth ratio of the ileum was observed in XOS-added groups(P<0.05).The high throughput sequencing analysis of bacterial 16S rRNA revealed that dietary XOS supplementation at 200 mg/kg altered cecal microbiota.Alpha diversity analysis illustrated a higher cecal bacterial richness in birds fed with XOS at 200 mg/kg.The composition of cecal microbiota modulated by the XOS addition was characterized by an increased abundance of Firmicutes along with a reduced abundance of Bacteroidetes.At the genus level,dietary XOS supple-mentation triggered decreases in Bacteroides and Campylobacter concurrent with increases in Lactoba-cillus and several short chain fatty acid producers including Desulfovibrio,Faecalitalea,Faecalicoccus,and 5 genera of family Lachnospiraceae.Collectively,dietary XOS addition improved the feed conversion ratio by modulating nutrient digestibility and ileal morphology in laying hens,which could be attributed to the enhancement of bacterial diversity and alteration of microbial composition.展开更多
The study was conducted to investigate the effect of essential oils on performance, egg quality, nutrient digestibility and yolk fatty acid profile in laying hens. A total of 960 Lohmann laying hens aged 53 weeks were...The study was conducted to investigate the effect of essential oils on performance, egg quality, nutrient digestibility and yolk fatty acid profile in laying hens. A total of 960 Lohmann laying hens aged 53 weeks were enrolled, under 4 different treatment diets supplemented with 0, 50.100 and 150 mg/kg essential oils(Enviva EO, Dupont Nutrition Biosciences ApS, Denmark), respectively. Each treatment was replicated8 times with 30 birds each. Birds were fed dietary treatment diets for 12 weeks(54 to 65 weeks). For data recording and analysis, a 12-week period was divided into 3 periods of 4 weeks' duration each: period 1(54 to 57 weeks), period 2(58 to 61 weeks), and period 3(62 to 65 weeks). For the diet supplemented with Enviva EO, hen-day egg production and the feed conversion ratio(FCR) were significantly improved(P < 0.05) at weeks 58 to 61, and the eggshell thickness was significantly increased(P < 0.05) at week 65.However, egg production, egg weight, feed intake, FCR and other egg quality parameters(albumen height, Haugh unit, egg yolk color and eggshell strength) were not affected by the dietary treatment. In addition, compared with the control diet, protein digestibility in the 100 mg/kg Enviva EO treatment group was significantly increased(P < 0.05), and fat digestibility in the 100 and 150 mg/kg Enviva EO treatment groups was significantly decreased(P < 0.05), but Enviva EO had no effect on energy apparent digestibility. Saturated fatty acid(SFA) and monounsaturated fatty acid(MUFA) gradually decreased and polyunsaturated fatty acid(PUFA) increased with Enviva EO supplementation, but the difference was not significant. The data suggested that the supplementation of essential oils(Enviva EO) in laying hen diet did not show a significant positive effect on performance and yolk fatty acid composition but it tended to increase eggshell thickness and protein digestibility, especially at the dose of 50 mg/kg.展开更多
The purpose of this study was to investigate the effects of dietary L-theanine supplementation on apparent nutrient digestibility,milk yield,milk composition,and blood biochemical indices of dairy cows under heat stre...The purpose of this study was to investigate the effects of dietary L-theanine supplementation on apparent nutrient digestibility,milk yield,milk composition,and blood biochemical indices of dairy cows under heat stress.Thirty Chinese Holstein cows(19.84±2.42 kg milk/d,192.36±40.77 d in milk and2±0.93 parities)were divided into 3 groups of 10 animals each.The control group was fed a basal total mixed ration(TMR)diet,while treatment 1(LTA16)and treatment 2(LTA32)groups were fed a basal TMR diet supplemented with L-theanine at 16 and 32 g/cow per day,respectively.The results showed that feeding the dairy cows with LTA16 treatment decreased(P0.05)on the dry matter intake,nutrient digestibility,total volatile fatty acid(TVFA)concentration and molar proportion of volatile fatty acid,milk yield,milk composition,feed efficiency and antioxidant capacity of the dairy cows.The triglyceride(TG)content of the LTA32 group was significantly greater(P=0.014)than that of the control group.With the increase in L-theanine dosage,the serum cholesterol(CHOL)content significantly increased(P=0.013).The serum albumin(ALB;P=0.067),low-density lipoprotein cholesterol(LDL-C;P=0.053),and high-density lipoprotein cholesterol(HDL-C;P=0.067)contents showed an upward trend as L-theanine dosage increased.Ultimately,the results of this study show that supplementing dairy cow diet with L-theanine could decrease dairy cow rectal temperature,affect lipid metabolism,and potentially relieve the heat stress of dairy cows to some extent.展开更多
The objective of the present study was to investigate the effects of methionine(Met) supplementation on growth performance of cubs, nutrient digestibility, nitrogen metabolism and serum biochemical parameters of femal...The objective of the present study was to investigate the effects of methionine(Met) supplementation on growth performance of cubs, nutrient digestibility, nitrogen metabolism and serum biochemical parameters of female blue foxes. One hundred primiparous female blue foxes that were similar in breeding date, pedigree, age, and weight were selected for the trial. The foxes were randomly assigned to four groups(n = 25 each group) and fed diets supplemented with Met at 2(Met2), 4(Met4), 6(Met6)and 8 g/kg(Met8), respectively, for 40 days. Our data showed that body weights at 20 and 40 d were significantly higher in the Met4 group than in the Met2 group(P < 0.05). The Met4 group also had the highest apparent digestibility of dry matter and crude protein compared with either the Met2, Met6, or Met8 group(P< 0.05). The serum Met and isoleucine(Ile) concentrations were significantly higher in the Met4 group than in the Met6 or Met8 group(P < 0.05). In summary, these data indicate that supplementary Met improves growth performance of cubs likely due to increased crude protein and dry matter and increased nitrogen retention of female blue foxes. The optimal amount of Met supplementation is10 g/kg basal diet.展开更多
In order to increase nutritive values of soybean meal(SBM), 3 species of microbes were used to ferment SBM. Through a 3 x 3 orthogonal design and parameter measurements of soybean peptide and antinutritional factor co...In order to increase nutritive values of soybean meal(SBM), 3 species of microbes were used to ferment SBM. Through a 3 x 3 orthogonal design and parameter measurements of soybean peptide and antinutritional factor contents in the fermented soybean meal(FSBM), it was estimated that the best microbial proportion of Bacillus subtilis, Hansenula anomala and Lactobacillus casei was 2:1:2 for SBM fermentation(P 0.05). However, newlyweaned piglets(d 28-38) fed 10% FSBM and different levels of plasma protein obtained higher average daily gain(ADG) and feed conversion ratio(FCR), compared with those without FSBM but with 6% plasma protein(P < 0.05). Piglets(d 38-68) fed diets supplemented with FSBM and soybean protein concentrate(SBPC) at 3.75% and 7.5% respectively increased nutrient digestibility,fecal enzyme activity and lactic acid bacteria counts, and decreased fecal Escherichia coli counts(P < 0.05), compared with the control. These data indicated that FSBM had positive effects on nutrient digestibility and fecal microflora for piglets.展开更多
Lauric acid(LA)has the possibility to improve milk production in dairy cows by improving mammary gland development,however,the mechanism by which it might regulate mammary gland development is unclear.The influence of...Lauric acid(LA)has the possibility to improve milk production in dairy cows by improving mammary gland development,however,the mechanism by which it might regulate mammary gland development is unclear.The influence of LA on milk production,nutrient digestibility and the expression of proteins related to mammary gland development in dairy cows were evaluated.Forty primiparous Holstein dairy cows were divided into 4 groups in a randomized block design.Four treatments included the control(0 g/d LA per cow),low-LA(100 g/d LA per cow),medium-LA(200 g/d LA per cow),and high-LA(300 g/d LA per cow).Yields of milk,fat-corrected milk,and energy-corrected milk quadratically increased(P<0.05),and yield and content of milk fat linearly increased(P<0.05)with LA supplementation.Percentages of C12:0,C18:1 and C20:1 fatty acids in milk fat linearly increased(P<0.05),but that of C16:0 fatty acid linearly decreased(P=0.046).Supplementation of LA led to a linear and quadratical increase(P<0.05)in digestibility of dry matter,organic matter,neutral detergent fibre and acid detergent fibre,and ruminal total volatile fatty acid concentration but a linear reduction(P=0.018)in the ratio of acetate to propionate.The enzymatic activities of ruminal pectinase,xylanase,andα-amylase,and populations of total bacteria and anaerobic fungi increased linearly(P<0.05),while populations of total protozoa and methanogens decreased linearly(P<0.05)with increased LA addition.Following LA addition,blood glucose,triglyceride,estradiol,prolactin,and insulin-like growth factor 1 concentrations increased linearly(P<0.05)and albumin and total protein concentrations increased quadratically(P<0.05).Moreover,addition of 200 g/d LA promoted(P<0.05)the expression of protein involved in mammary gland development and fatty acids synthesis.These results suggested that LA addition enhanced milk production and fatty acids synthesis by stimulating nutrient digestion,the expression of proteins associated with milk fat synthesis and mammary gland development.展开更多
Background Addressing the shortage of high-quality protein resources,this study was conducted to investigate the effects of replacing soybean meal(SBM)with different levels of enzymolysis-fe rmentation compound protei...Background Addressing the shortage of high-quality protein resources,this study was conducted to investigate the effects of replacing soybean meal(SBM)with different levels of enzymolysis-fe rmentation compound protein feed(EFCP)in the diets of growing—finishing pigs,focusing on growth perfo rmance,nutrients digestibility;carcass traits,and meat quality.Methods Sixty DLY(Durdcx LandracexYorkshire)pigs with an initial body weight of 42.76±2.05 kg were assigto 5 diietary treatments in a 2×2+1 factorial design.These dietary treatments included a corn-soybean meal diet(CON),untreated compound protein feed(UCP)substitution 50%(U50)and 100%SBM(U100)diets,and EFCP substitution 50%(EF50)and 100%SBM(EF100)diets.Each treatment had 6 pens(replicates)with 2 pigs per pen,and the experiment lasted 58 d,divided into phaseⅠ(1-28 d)and phaseⅡ(29-58 d).Following phaseⅠ,only the CON,U50,and EF50 groups were continued for phaseⅡ,each with 5 replicate pens.On d 59,a total of 15 pigs(1 pig/pen,5pensreatment)were euthanized.Results During phaseⅠ,the EF50 group had a higher average daily gain(ADG)in pigs(P<0.05)compared to the CON group,whereas the U50 group did not have a significant difference.As the substitution ratio of UCP and EFCP increased in phaseⅠ,there was a noticeable reduction in the final body weight and ADG(P<0.05),along with an increase in the feed-to-gain ratio(F/G)(P<0.05).In phaseⅡ,there were no significant differences in growth performance among the treatment groups,but EF50 increased the apparent digestibility of several nutrients(including dry matter,crude protein,crude fiber,acid detergent fiber,ash,gross energy)compared to U50.The EF50 group also exhibited significantly higher serum levels of neuropeptide Y and ghrelin compared to the CON and U50 groups(P<0.05),Moreover,the EF50 group had higher carcass weight and carcass length than those in the CON and U50groups(P<0.05),with no significant difference in meat quality.Conclusions The study findings suggest that replacing 50%SBM with EFCP during the growing-finishing period can improve the growth performance,nutrient digestibility,and carcass traits of pigs without compromising meat quality This research offers valuable insights into the modification of unconventional plant protein meals and developing alternatives to SBM.展开更多
Twenty-eight male, weaned Chinese Holstein calves((156.8±33.4) kg) were used to investigate the effects of dietary forage to concentrate ratio(F:C) and forage length on nutrient digestibility, plasma metab...Twenty-eight male, weaned Chinese Holstein calves((156.8±33.4) kg) were used to investigate the effects of dietary forage to concentrate ratio(F:C) and forage length on nutrient digestibility, plasma metabolites, ruminal fermentation, and fecal microflora. Animals were randomly allocated to four treatments in a 2×2 factorial arrangement: whole-length forage(WL) with low F:C(50:50); WL with high F:C(65:35); short-length forage(SL) with high F:C(65:35); and SL with low F:C(50:50). Chinese wildrye was used as the only forage source in this trial. The grass in the SL treatments was chopped using a chaff cutter to achieve small particle size(-50% particles 〉19 mm). Dry matter intake(DMI) and organic matter(OM) intake was increased by increasing both F:C(P〈0.01) and forage length(FL)(P〈0.05), while acid detergent fiber(ADF) and neutral detergent fiber(NDF) intakes were only increased by increasing the F:C(P〈0.01). The digestibility of NDF was increased as the FL increased(P〈0.01), and it was also affected by interaction between F:C and FL(P〈0.05). Cholesterol(CHO)(P〈0.01), leptin(LP)(P〈0.05), and growth hormone(GH)(P〈0.01) concentrations in plasma were increased as dietary F:C increased. A significant increase in plasma triglyceride(TG)(P〈0.01), insulin(INS)(P〈0.05), and GH(P〈0.01) levels was observed with decreasing dietary FL. Ruminal p H values of calves fed with low F:C diets were significantly lower than those in high F:C treatment(P〈0.05). Increasing the F:C enhanced ruminal acetic acid(P〈0.05) and acetic acid/propionic acid(P〈0.01). Fecal Lactobacillus content was significantly higher, while Escherichia coli and Salmonella contents were significantly lower in WL and high F:C groups(P〈0.05). Lower fecal scores(higher diarrhea rate) were observed in calves fed with SL hay compared to WL hay(P〈0.05). Denatured gradient gel electrophoresis(DGGE) bands and richness index(S) were significantly affected by the interaction between F:C and FL(P〈0.05), under high F:C, band numbers and richness index from WL group were higher than that from SL group(P〈0.05), whereas there were no differences between WL andSL groups under low F:C(P〉0.05). Microflora similarity was 50–73% among the different treatments. It is concluded that the WL with high F:C(65:35) diet is suitable for weaned calves.展开更多
The nutritive quality of guar (Cyamopsis tetragonoloba) residues (GR) was investigated and the effects of partial replacement of wheat straw by GR on nutrients intake, digestibility, and growth performance. Twenty fou...The nutritive quality of guar (Cyamopsis tetragonoloba) residues (GR) was investigated and the effects of partial replacement of wheat straw by GR on nutrients intake, digestibility, and growth performance. Twenty four male lambs, with average body weight (BW) of 40.3 ± 2.5 kg were divided into three groups. Lambs were fed individually for 104 days with concentrate mixture (18% crude protein) and wheat straw as a total mixed ration. Wheat straw was replaced with 0, 100 and 145 g/kg dry matter guar residues. All rations were isonitrogenous and isocaloric. The fattening trial lasted 104 days. At the end of week 10 of the trail, a digestion trial was performed using six lambs from each group. The composition of nutrients in the GR was 87%, 8.5%, 56.4% and 40.1% for DM, CP, NDF and ADF, respectively. DM and crude protein (CP) intake were not affected by the inclusion of GR. However, neutral detergent fibre (NDF) and acid detergent fibre (ADF) intake were lower in lambs fed GR compared to control lambs. At the end of the experiment, lambs fed 100 and 145 g GR/kg DM diets gained more weight (P version (CR). Results from this work suggested that GR had advantages compared to regular roughage (wheat straw) in regard to parameters investigated as well as the significant reduction in fattening diets cost.展开更多
Background Given the high incidence of gastrointestinal disorders in intensive rabbit production,we assessed the effects of graded levels of xylooligosaccharides(XOS)on growth performance,nutrient digestibility and in...Background Given the high incidence of gastrointestinal disorders in intensive rabbit production,we assessed the effects of graded levels of xylooligosaccharides(XOS)on growth performance,nutrient digestibility and intestinal health in growing rabbits.Methods The 35-day-old weaned rabbits(889.41±0.41 g)were randomly assigned to five dietary treatments(0,0.2,0.3,0.4 or 0.5 g/kg XOS)and the trial lasted for 35 d.Results The results revealed that linear trend responses of body weight(BW)to XOS on d 21 and 35(P≤0.05).During d 1-21,0.2 g/kg XOS increased average daily feed intake(ADFI)while 0.5 g/kg improved feed conversion ratio(FCR)significantly(P≤0.05).Weight gain rate(WGR)showed a linear trend,while FCR showed a quadratic response(P≤0.05).Throughout the 35-d trial,0.2 and 0.3 g/kg XOS enhanced ADFI,and 0.4 g/kg XOS improved FCR significantly,average daily gain(ADG)demonstrated linear dose-responsiveness,while WGR and FCR showed quadratic trends(P≤0.05).Notably,0.2 g/kg XOS elevated serum glutathione peroxidase(GSH-Px)activity and ileal secretory immunoglobulin A(sIgA)levels.Furthermore,0.3,0.4 and 0.5 g/kg XOS reduced jejunal malonaldehyde(MDA)content,0.4 g/kg XOS decreased serum MDA,and 0.5 g/kg XOS elevated serum immunoglobulin M(IgM)significantly(P≤0.05).0.2,0.4,0.5 g/kg XOS improved the digestibility of crude fiber(CF),0.2 and 0.4 g/kg XOS increased acid detergent fiber(ADF),and neutral detergent fiber(NDF)also increased among all treatments,although 0.5 g/kg XOS reduced cellulase activity significantly(P≤0.05).Furthermore,graded levels of XOS significantly changed the relative abundance of specific bacteria,and 0.4 and 0.5 g/kg XOS enhanced the content of valeric acid significantly(P≤0.05).Conclusions In conclusion,dietary supplementation of XOS serves as an effective nutritional strategy to optimize bacterial community in the cecum,improve fiber digestion and valeric acid production,while enhances resistance to intestinal pathogen infection and oxidative stress in rabbit production.展开更多
基金financially supported by the National Key Technology R&D Program in the 11th Five Yr Plan of China(2006BAD12B01)Special Public Sector Fund in Agriculture(200903006)
摘要The objective of this study was to investigate the effects of total dietary fiber level on nutrient digestibility and the relationship between apparent total tract digestibility of total dietary fiber, and soluble dietary fiber, insoluble dietary fiber and available energy. Sugar beet pulp was as the only fiber source. The experiment was designed as a 6 x 6 Latin square with an adaptation period of 7 d followed by a 5-d total collection of feces and urine. Feed intake tended to decrease (P =0.] O) as total dietary fiber level increased. The apparent total tract digestibility of dry matter, crude protein and gross energy decreased (P 〈0.0]) when total dietary fiber increased but the digestibility of soluble dietary fiber and insoluble dietary fiber increased (P 〈0.01). The digestible energy and metabolizable energy content of diets decreased (P 〈0.01) as the total dietary fiber increased.
基金funded by Project of National Natural Science Foundation of China(No.32172744 and 31902165)Natural Science Foundation of Guangdong Province(2021A1515012116)Natural Science Foundation of Guangzhou City(202102080090).
摘要Background:This study aimed to investigate the hydration properties of different-source fibrous materials by com-paring their water-binding capacity(WBC),water swelling capacity(WSC),viscosity,and in vivo effects of selected samples on growth performance,nutrient digestibility,diarrhea,and intestinal health in weaned piglets.Methods:A total of 13 commercially available fibrous materials were first compared in chemical composition and in vitro hydration property.Subsequently,40 weaned piglets were randomized to five experimental dietary groups(8 piglets per group):control diet(a basal diet without dietary fiber,CON),basal diet supplemented with 5%microcrys-talline cellulose(MCC),5%wheat bran(WB),5%Moringa oleifera leaf powder(MOLP),or 5%sugar beet pulp(SBP),fol-lowed by analyzing their growth performance and diarrhea rate in a 28-d experiment.After the feeding experiment,anaesthetized piglets were killed,and their intestinal and colon content or plasma samples were analyzed in nutrient digestibility,intestinal morphology,intestinal barrier,short-chain fatty acids(SCFAs),and bacterial population.Results:In vitro studies showed low hydration properties for WB and MCC,while medium hydration properties for MOLP and SBP.In vivo studies indicated that compared with medium hydration property groups,low hydration prop-erty groups showed(1)exacerbated diarrhea,impaired intestinal health,and reduced apparent fecal digestibility of dry matter,gross energy,acid detergent fiber,and neutral detergent fiber;(2)decreased SCFAs concentration and rela-tive levels of Lactobacillus and Bifidobacterium,but increased levels of Escherichia coli and Brachyspira hyodysenteriae in colon contents.Additionally,SBP showed optimal performance in reducing diarrhea and increasing SCFAs produc-tion.Correlation analysis revealed a positive correlation of fiber hydration properties with in vitro SCFAs production,and diarrhea index and nutrient digestibility were negatively and positively correlated with SCFAs levels in the colon contents of weaned piglets,respectively.Conclusions:Different-source dietary fibers varied in their hydration properties and impacts on diarrhea,microbial composition and SCFAs production in weaned piglets.WB and MCC could exacerbate diarrhea and impair nutrient digestibility,probably because their low hydration properties were detrimental to gut microbial homeostasis and fermentation.Our findings provide new ideas for rational use of fiber resources in weaned piglets.
基金financially supported by the Special Fund for Agro-scientific Research in the Public Interest of China(201003020)the Guangdong University Innovation Talents Cultivating Project of China(1009324)+1 种基金the Guangdong Natural Science Foundation of China(S2012 040007863)by the Guangdong Province Universities and College Pearl River Scholar Funded Scheme(GD UPS-2011)
摘要Extruded soybean meal (ESBM) was evaluated as a protein source for partial replacement of fish meal (FM) in diets of juvenile Litopenaeus vannarnei. In the control diet (Diet 1), FM protein was replaced with increasing dietary levels of ESBM (4.28%, 8.40%, 12.62%, 16.82%, and 25.26%) at 10%, 20%, 30%, 40%, and 60% levels (Diets 2 to 6, respectively). An eight-week feeding trial was conducted on 720 juvenile shrimp (0.67 g ± 0.01 g mean initial Weight), and nutrient digestibility of the six diets was determined. ESBM could replace 20% of FM without causing a significant reduction in growth of shrimp, but other dietary treatments strongly affected whole body composition. Crude protein content of the whole body fed Diet 6 was significantly lower than that fed Diet 2 (P〈0.05), while crude lipid content of the whole body fed Diet 5 or 6 was significantly higher than that fed Diet 2 (P〈0,05). Protein digestibilities of Diets 5 and 6 were significantly lower than that of Diet 1 (P〈0.05). Digestibility of lipids ranged from 96.97% in Diet 6 to 98.34% in Diet 3, whereas dry matter digestibility decreased with increasing replacement level. This study indicates that 20% FM replacement with ESBM in the basic diet containing 40% protein and 30% FM is optimal for juvenile L. vannamei.
基金support received from the State Key Laboratory of Animal Nutrition of China ( No .2004DA125184-0810)National Natural Science Foundation of China(No .31072040)+1 种基金Guangdong Cooperation Project be-tween Industry-Academia-Research of China ( No .2009B090300110)the Ministry of Science and Technology of the People’s Republic of China ( No .2006BAD12B05-10and No . Nyhyzx07-34)
摘要A total of 480 nursery pigs (Duroc x Landrace x Large White) were utilized in two experiments conducted to determine the effects of different ratios of standardized ileal digestible lysine (SID- Lys) to metabolizable energy (ME) ratio on the performance, nutrient digestibility, plasma urea nitrogen (PUN) ,and plasma free amino acids of 10 to 28 kg pigs. In Exp. 1, 192 pigs ( 10.58 kg) were assigned to one of four treatments. The treatments consisted of diets with a ME content of 3.2, 3.25, 3.3, or 3.35 Mcal/kg with a constant SID-Lys:ME ratio of 3.7 g/Mcal. The experiment lasted 28 days. Pigs which were fed the diets containing 3.3 and 3.35 Mcal/kg ME had lower feed intakes ( P 〈 0.05)than those fed 3.2 Mcal/kg. Feed efficiency was linearly improved with increasing dietary ME ( P 〈0.05). Increasing the dietary ME level also increased (P 〈0.05 ) dry matter and energy digestibility. Therefore,3.3 Mcal/kg ME was selected for Exp. 2 in which 288 pigs ( 10.60 kg) were assigned to one of six treatments. Treatments consisted of SID-Lys: ME ratios of 3.1,3.3,3.5,3.7,3.9, or 4.1 g/Mcal with all diets providing 3.3 Mcal of ME/kg. Weight gain and feed efficiency were increased (P 〈0.05 ) as the SID-Lys:ME ratio in diet increased. Based on a straight broken-line model ,the estimated SID-Lys:ME ratio to maximize weight gain was 3.74.
基金Supported by Suzhou Agricultural Science and Technology Support Program(SNG201233)
摘要The experiment was conducted to study the effects of low protein diets on nutrient digestibility and excretion of finishing pigs. For the 30-d trial,fortytwo duroc × landrace × yorkshire( DLY) finishing pigs with the initial weight of( 91. 13 ± 1. 64) kg were randomly divided into two groups,with three replicates in each group. The pigs in the two groups were fed with different crude protein( CP) levels( control groups: 15. 49% and low protein groups: 12. 59%). The results showed that:( 1) The digestibility of dry matter and crude fat( CF) in low protein groups were increased significantly( P 0. 05). The results showed that 2. 9% reduction of the CP in diets could improve nutrient digestibility and reduce the excretion of N,but reduced the digestibility of Arg.
摘要This experiment was conducted to determine the effects of a commercial enzyme mixture providing xylanase and protease activities on the digestibility of dietary nutrients when added to diets based on peas and wheat and fed to 9. 5 to 15 kg pigs. Pigs were weaned at 18 to 21 days of age and fitted with a simple T-cannula at the distal ileum,6 to 7 days after weaning. After 5 to 7 days for adaptation to their diets, ileal digesta, and feces were collected. Enzyme supplementation had no effect on the apparent ileal or fecal digestibility of any dietary nutrient. There was a numerical improvement ( P 〉 0.05 ) in the ileal digestibility of fiber. In conclusion, xylanase and protease appeared to be ineffective in improving nutrient digestibility when supplemented in diets based on peas and wheat fed to weaned pigs.
摘要The study aimed at evaluating the effect of supplementing Saccharomyces cerevisiae into low quality local-based feeds on performance and nutrient digestibility of late starter to grower stage local pigs. There were total 16 late starter local pigs fed with four treatment feeds based on block design with four pigs in each treatment. The four treatment feeds offered consisted of: commercial starter feeds Charoen Pokphand 552 (To), basal feeds + yeast 2% of daily feeds requirement (T0, basal feeds + yeast 4% of daily feeds requirement (T2) and basal feeds + yeast 6% of daily feeds requirement (T3). Feed intake, daily weight gain, feed conversion efficiency, protein and crude fiber digestibility were evaluated in the study. The findings showed that supplementing S. cerevisiae into low quality local-based diet improved 0.9%-2.7% feeds' crude protein, 10%-19% feeds intake, 1%-6% daily weight gain, 3%-4% crude protein digestibility and 4%-5% crude fiber digestibility, but reduced feeds conversion efficiency by 0.3-0.4. The conclusion drawn is that supplementing S. cerevisiae (yeast) up to 6% improved performances of starter local pigs fed low quality feeds and performed the similar result with feeding commercial starter feeds Charoen Pokphand 552. Further research by widening the range and increasing the level of yeast supplementation could be done.
摘要One hundred and twenty day-old Anak 2,000 broilers were used to test the effect of replacing maize with cornflakes waste meal (CFWM) on their performance and apparent nutrient digestibility in a complete randomized design. Five dietary, treatments ( l, 2, 3, 4 and 5) were lbnnulated and CFWM was included in the diet in place of maize at concentrations of 0, 25, 50, 75 and 100% respectively. The results revealed that dietary, inclusion of CFWM from 25%-75% significantly (P 〈 0.05) increased average live weight and weight gain, bnt reduced the teed intake and improved the feed to gain ratio of birds compared to those on the control diet (0% CFWM). Besides, apparent digestible crude fibre, ether extract and ash of broilers on the control diet were lower (P 〈 0.05) thm~ those on CFWM based diets. The overall results show that CFWM can successfully replace maize tip to 75% levels as all energ) source with significant improvement in growth rate and nutrient retention of broiler starters.
基金supported by National Basic Research Program of China(2013CB127301)National Scientific and Technology Support Project(2013BAD21B04)Guangdong Province Natural Science Foundation of China(Project No.S2013010013215)
摘要This study was conducted to investigate the effect of dietary protease supplementation on the growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression in weaned piglets. A total of 300 weaned piglets(21 days of age Duroc x Large White x Landrace; initial BW = 6.27 ± 0.45 kg) were randomly divided into 5 groups. The 5 diets were: 1) positive control diet(PC), 2) negative control diet(NC), and 3) protease supplementations, which were 100, 200, and 300 mg per kg NC diet. Results indicated that final BW, ADG, ADFI, crude protein digestibility, enzyme activities of stomach pepsin, pancreatic amylase and trypsin, plasma total protein, and intestinal villus height were higher for the PC diet and the supplementations of 200 and 300 mg protease per kg NC diet than for the NC diet(P < 0.05). Supplementations of 200 and 300 mg protease per kg NC diet significantly increased the ratio of villus height to crypt depth(VH:CD) of duodenum, jejunum and ileum compared with NC diet(P < 0.05). Feed to gain ratio, diarrhea index, blood urea nitrogen, and diamine oxidase were lower for the PC diet and supplementations of 200 and 300 mg protease per kg NC diet than for the NC diet(P < 0.05). Piglets fed the PC diet had a higher peptide transporter 1(PepTl) mRNA abundance in duodenum than piglets fed the NC diet(P < 0.05), and supplementations of 100, 200 and 300 mg protease per kg NC diet increased the PepTl mRNA abundance in duodenum(P < 0.05) comparing with the NC diet. Piglets fed the PC diet had a higher b0,+AT mRNA abundance in jejunum than piglets fed the NC diet(P < 0.05), and supplementations of 200 and 300 mg protease per kg NC diet increased the b0,+AT mRNA abundance in jejunum and ileum comparing with the NC diet(P < 0.05). In summary, dietary protease supplementation increases growth performance in weaned piglets, which may contribute to the improvement of intestinal development, protein digestibility, nutrient transport efficiency, and health status of piglets when fed low digestible protein sources.
基金This work was funded by the Key Research and Development Program of Sichuan Province(no.2020YFN0147)the National Natural Science Foundation of China(no.31972599).
摘要To explore the effects of fermented rapeseed meal(FRSM)on growth performance and intestinal health,a total of 30 growing pigs were randomly allotted to three treatments consisting of corn-soybean meal diet(CSD),rapeseed meal diet(RSD),and fermented rapeseed meal diet(FRSD).Results showed that compared with RSD,FRSD feeding increased the average daily gain and final body weight in pigs(P<0.01).Compared with RSD feeding,FRSD feeding elevated the apparent digestibility of crude protein,acid detergent fiber,and ether extract in pigs(P<0.01).Moreover,the FRSD group exhibited greater apparent ileal digestibility of His,Thr,Lys,and Ser than the RSD group(P<0.01).The digestible energy,metabolic energy,and nitrogen utilization were higher in the FRSD and CSD groups than in the RSD group(P<0.01).As compared to the RSD,FRSD feeding decreased the serum concentration of leptin but significantly increased the concentrations of immunoglobulin(Ig)A,IgG,ghrelin,and enzyme activities of amylase,lipase,and trypsin in the pancreas(P<0.05).Interestingly,the villus height,the ratio of villus height to crypt depth,and the activities of brush border enzymes(e.g.,maltase and sucrase)in the small intestine were higher in the CSD and FRSD groups than in the RSD group(P<0.05).As compared to the RSD,the FRSD feeding not only increased the expression level of the occludin in the small intestinal epithelium(P<0.05)but also elevated the expression levels of claudin-1,MUC1,and PepT1 genes in the duodenum,and elevated the expression levels of SGLT1 and CAT1 genes in the jejunum(P<0.05).Importantly,FRSD feeding significantly decreased the abundance of Escherichia coli,but increased the abundance of Lactobacillus and the content of butyrate in the cecum and colon(P<0.05).These results indicated that compared with rapeseed meal,fermented rapeseed meal exhibited a positive effect on improving the growth performance and intestinal health in growing pigs,and the results may also help develop novel protein sources for animal nutrition and the feed industry.
基金National Key R&D Program of China(2018YFD0500603)China Agricultural Research System(CARS40-K12)the China Agriculture Research System-Beijing Team for Poultry Industry and the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences.
摘要This study was conducted to evaluate the prebiotic effects of dietary xylooligosaccharide(XOS)sup-plementation on performance,nutrient digestibility,intestinal morphology,and gut microbiota in laying hens.In a 12-wk experiment,a total of 288 Hy-Line Brown layers at 50 wk of age were randomly assigned into 3 dietary treatments supplemented with XOS at 0,200 or 400 mg/kg.Each treatment had 8 replicates with 12 birds each.Hens fed XOS diets showed a lower feed-to-egg ratio during wk 7 to 12 and a higher egg yolk color value in wk 12 compared with those fed the control diet(P<0.05).Dietary XOS supplementation improved the apparent total tract digestibility of gross energy and nitrogen at the end of the 12th wk(P<0.05).In addition,a higher villus height-to-crypt depth ratio of the ileum was observed in XOS-added groups(P<0.05).The high throughput sequencing analysis of bacterial 16S rRNA revealed that dietary XOS supplementation at 200 mg/kg altered cecal microbiota.Alpha diversity analysis illustrated a higher cecal bacterial richness in birds fed with XOS at 200 mg/kg.The composition of cecal microbiota modulated by the XOS addition was characterized by an increased abundance of Firmicutes along with a reduced abundance of Bacteroidetes.At the genus level,dietary XOS supple-mentation triggered decreases in Bacteroides and Campylobacter concurrent with increases in Lactoba-cillus and several short chain fatty acid producers including Desulfovibrio,Faecalitalea,Faecalicoccus,and 5 genera of family Lachnospiraceae.Collectively,dietary XOS addition improved the feed conversion ratio by modulating nutrient digestibility and ileal morphology in laying hens,which could be attributed to the enhancement of bacterial diversity and alteration of microbial composition.
基金supported by Dupont Nutrition Biosciences ApS and Specific Research Team Supporting program of Sichuan Agricultural University
摘要The study was conducted to investigate the effect of essential oils on performance, egg quality, nutrient digestibility and yolk fatty acid profile in laying hens. A total of 960 Lohmann laying hens aged 53 weeks were enrolled, under 4 different treatment diets supplemented with 0, 50.100 and 150 mg/kg essential oils(Enviva EO, Dupont Nutrition Biosciences ApS, Denmark), respectively. Each treatment was replicated8 times with 30 birds each. Birds were fed dietary treatment diets for 12 weeks(54 to 65 weeks). For data recording and analysis, a 12-week period was divided into 3 periods of 4 weeks' duration each: period 1(54 to 57 weeks), period 2(58 to 61 weeks), and period 3(62 to 65 weeks). For the diet supplemented with Enviva EO, hen-day egg production and the feed conversion ratio(FCR) were significantly improved(P < 0.05) at weeks 58 to 61, and the eggshell thickness was significantly increased(P < 0.05) at week 65.However, egg production, egg weight, feed intake, FCR and other egg quality parameters(albumen height, Haugh unit, egg yolk color and eggshell strength) were not affected by the dietary treatment. In addition, compared with the control diet, protein digestibility in the 100 mg/kg Enviva EO treatment group was significantly increased(P < 0.05), and fat digestibility in the 100 and 150 mg/kg Enviva EO treatment groups was significantly decreased(P < 0.05), but Enviva EO had no effect on energy apparent digestibility. Saturated fatty acid(SFA) and monounsaturated fatty acid(MUFA) gradually decreased and polyunsaturated fatty acid(PUFA) increased with Enviva EO supplementation, but the difference was not significant. The data suggested that the supplementation of essential oils(Enviva EO) in laying hen diet did not show a significant positive effect on performance and yolk fatty acid composition but it tended to increase eggshell thickness and protein digestibility, especially at the dose of 50 mg/kg.
基金financially supported by National Key Research and Development Program(Grant No.2018YFD0501604)Hunan Provincial Science and Technology Major Project(Grant No.2017NK1020)+3 种基金Hunan Provincial Natural Science Foundation(Grant No.2019JJ50279)The Construction of Innovative in Hunan Province(Grant No.2019RS3021)Hunan Agricultural University(Grant No.17QN09)Effect of secondary metabolites of rapeseed meal on methane emission from goats(Grant No.2019ZDSYS01)。
摘要The purpose of this study was to investigate the effects of dietary L-theanine supplementation on apparent nutrient digestibility,milk yield,milk composition,and blood biochemical indices of dairy cows under heat stress.Thirty Chinese Holstein cows(19.84±2.42 kg milk/d,192.36±40.77 d in milk and2±0.93 parities)were divided into 3 groups of 10 animals each.The control group was fed a basal total mixed ration(TMR)diet,while treatment 1(LTA16)and treatment 2(LTA32)groups were fed a basal TMR diet supplemented with L-theanine at 16 and 32 g/cow per day,respectively.The results showed that feeding the dairy cows with LTA16 treatment decreased(P0.05)on the dry matter intake,nutrient digestibility,total volatile fatty acid(TVFA)concentration and molar proportion of volatile fatty acid,milk yield,milk composition,feed efficiency and antioxidant capacity of the dairy cows.The triglyceride(TG)content of the LTA32 group was significantly greater(P=0.014)than that of the control group.With the increase in L-theanine dosage,the serum cholesterol(CHOL)content significantly increased(P=0.013).The serum albumin(ALB;P=0.067),low-density lipoprotein cholesterol(LDL-C;P=0.053),and high-density lipoprotein cholesterol(HDL-C;P=0.067)contents showed an upward trend as L-theanine dosage increased.Ultimately,the results of this study show that supplementing dairy cow diet with L-theanine could decrease dairy cow rectal temperature,affect lipid metabolism,and potentially relieve the heat stress of dairy cows to some extent.
摘要The objective of the present study was to investigate the effects of methionine(Met) supplementation on growth performance of cubs, nutrient digestibility, nitrogen metabolism and serum biochemical parameters of female blue foxes. One hundred primiparous female blue foxes that were similar in breeding date, pedigree, age, and weight were selected for the trial. The foxes were randomly assigned to four groups(n = 25 each group) and fed diets supplemented with Met at 2(Met2), 4(Met4), 6(Met6)and 8 g/kg(Met8), respectively, for 40 days. Our data showed that body weights at 20 and 40 d were significantly higher in the Met4 group than in the Met2 group(P < 0.05). The Met4 group also had the highest apparent digestibility of dry matter and crude protein compared with either the Met2, Met6, or Met8 group(P< 0.05). The serum Met and isoleucine(Ile) concentrations were significantly higher in the Met4 group than in the Met6 or Met8 group(P < 0.05). In summary, these data indicate that supplementary Met improves growth performance of cubs likely due to increased crude protein and dry matter and increased nitrogen retention of female blue foxes. The optimal amount of Met supplementation is10 g/kg basal diet.
摘要In order to increase nutritive values of soybean meal(SBM), 3 species of microbes were used to ferment SBM. Through a 3 x 3 orthogonal design and parameter measurements of soybean peptide and antinutritional factor contents in the fermented soybean meal(FSBM), it was estimated that the best microbial proportion of Bacillus subtilis, Hansenula anomala and Lactobacillus casei was 2:1:2 for SBM fermentation(P 0.05). However, newlyweaned piglets(d 28-38) fed 10% FSBM and different levels of plasma protein obtained higher average daily gain(ADG) and feed conversion ratio(FCR), compared with those without FSBM but with 6% plasma protein(P < 0.05). Piglets(d 38-68) fed diets supplemented with FSBM and soybean protein concentrate(SBPC) at 3.75% and 7.5% respectively increased nutrient digestibility,fecal enzyme activity and lactic acid bacteria counts, and decreased fecal Escherichia coli counts(P < 0.05), compared with the control. These data indicated that FSBM had positive effects on nutrient digestibility and fecal microflora for piglets.
基金funded by the Education Department of Shanxi Province,Excellent Doctor Work Award Fund Research Project[SXYBKY2018036]Modern Agro industry Technology Research System of Shanxi Province (2023CYJSTX13).
摘要Lauric acid(LA)has the possibility to improve milk production in dairy cows by improving mammary gland development,however,the mechanism by which it might regulate mammary gland development is unclear.The influence of LA on milk production,nutrient digestibility and the expression of proteins related to mammary gland development in dairy cows were evaluated.Forty primiparous Holstein dairy cows were divided into 4 groups in a randomized block design.Four treatments included the control(0 g/d LA per cow),low-LA(100 g/d LA per cow),medium-LA(200 g/d LA per cow),and high-LA(300 g/d LA per cow).Yields of milk,fat-corrected milk,and energy-corrected milk quadratically increased(P<0.05),and yield and content of milk fat linearly increased(P<0.05)with LA supplementation.Percentages of C12:0,C18:1 and C20:1 fatty acids in milk fat linearly increased(P<0.05),but that of C16:0 fatty acid linearly decreased(P=0.046).Supplementation of LA led to a linear and quadratical increase(P<0.05)in digestibility of dry matter,organic matter,neutral detergent fibre and acid detergent fibre,and ruminal total volatile fatty acid concentration but a linear reduction(P=0.018)in the ratio of acetate to propionate.The enzymatic activities of ruminal pectinase,xylanase,andα-amylase,and populations of total bacteria and anaerobic fungi increased linearly(P<0.05),while populations of total protozoa and methanogens decreased linearly(P<0.05)with increased LA addition.Following LA addition,blood glucose,triglyceride,estradiol,prolactin,and insulin-like growth factor 1 concentrations increased linearly(P<0.05)and albumin and total protein concentrations increased quadratically(P<0.05).Moreover,addition of 200 g/d LA promoted(P<0.05)the expression of protein involved in mammary gland development and fatty acids synthesis.These results suggested that LA addition enhanced milk production and fatty acids synthesis by stimulating nutrient digestion,the expression of proteins associated with milk fat synthesis and mammary gland development.
基金supported by the Major Science and Technology Program of Sichuan Province(No.2021ZDZX0009)Key Research and Development Program of Sichuan Province(No.2020YFN0147)。
摘要Background Addressing the shortage of high-quality protein resources,this study was conducted to investigate the effects of replacing soybean meal(SBM)with different levels of enzymolysis-fe rmentation compound protein feed(EFCP)in the diets of growing—finishing pigs,focusing on growth perfo rmance,nutrients digestibility;carcass traits,and meat quality.Methods Sixty DLY(Durdcx LandracexYorkshire)pigs with an initial body weight of 42.76±2.05 kg were assigto 5 diietary treatments in a 2×2+1 factorial design.These dietary treatments included a corn-soybean meal diet(CON),untreated compound protein feed(UCP)substitution 50%(U50)and 100%SBM(U100)diets,and EFCP substitution 50%(EF50)and 100%SBM(EF100)diets.Each treatment had 6 pens(replicates)with 2 pigs per pen,and the experiment lasted 58 d,divided into phaseⅠ(1-28 d)and phaseⅡ(29-58 d).Following phaseⅠ,only the CON,U50,and EF50 groups were continued for phaseⅡ,each with 5 replicate pens.On d 59,a total of 15 pigs(1 pig/pen,5pensreatment)were euthanized.Results During phaseⅠ,the EF50 group had a higher average daily gain(ADG)in pigs(P<0.05)compared to the CON group,whereas the U50 group did not have a significant difference.As the substitution ratio of UCP and EFCP increased in phaseⅠ,there was a noticeable reduction in the final body weight and ADG(P<0.05),along with an increase in the feed-to-gain ratio(F/G)(P<0.05).In phaseⅡ,there were no significant differences in growth performance among the treatment groups,but EF50 increased the apparent digestibility of several nutrients(including dry matter,crude protein,crude fiber,acid detergent fiber,ash,gross energy)compared to U50.The EF50 group also exhibited significantly higher serum levels of neuropeptide Y and ghrelin compared to the CON and U50 groups(P<0.05),Moreover,the EF50 group had higher carcass weight and carcass length than those in the CON and U50groups(P<0.05),with no significant difference in meat quality.Conclusions The study findings suggest that replacing 50%SBM with EFCP during the growing-finishing period can improve the growth performance,nutrient digestibility,and carcass traits of pigs without compromising meat quality This research offers valuable insights into the modification of unconventional plant protein meals and developing alternatives to SBM.
基金supported by the earmarked fund for China Agriculture Research System (CARS-37)Special Fund for Agro-scientific Research in the Public Interest (201303144)
摘要Twenty-eight male, weaned Chinese Holstein calves((156.8±33.4) kg) were used to investigate the effects of dietary forage to concentrate ratio(F:C) and forage length on nutrient digestibility, plasma metabolites, ruminal fermentation, and fecal microflora. Animals were randomly allocated to four treatments in a 2×2 factorial arrangement: whole-length forage(WL) with low F:C(50:50); WL with high F:C(65:35); short-length forage(SL) with high F:C(65:35); and SL with low F:C(50:50). Chinese wildrye was used as the only forage source in this trial. The grass in the SL treatments was chopped using a chaff cutter to achieve small particle size(-50% particles 〉19 mm). Dry matter intake(DMI) and organic matter(OM) intake was increased by increasing both F:C(P〈0.01) and forage length(FL)(P〈0.05), while acid detergent fiber(ADF) and neutral detergent fiber(NDF) intakes were only increased by increasing the F:C(P〈0.01). The digestibility of NDF was increased as the FL increased(P〈0.01), and it was also affected by interaction between F:C and FL(P〈0.05). Cholesterol(CHO)(P〈0.01), leptin(LP)(P〈0.05), and growth hormone(GH)(P〈0.01) concentrations in plasma were increased as dietary F:C increased. A significant increase in plasma triglyceride(TG)(P〈0.01), insulin(INS)(P〈0.05), and GH(P〈0.01) levels was observed with decreasing dietary FL. Ruminal p H values of calves fed with low F:C diets were significantly lower than those in high F:C treatment(P〈0.05). Increasing the F:C enhanced ruminal acetic acid(P〈0.05) and acetic acid/propionic acid(P〈0.01). Fecal Lactobacillus content was significantly higher, while Escherichia coli and Salmonella contents were significantly lower in WL and high F:C groups(P〈0.05). Lower fecal scores(higher diarrhea rate) were observed in calves fed with SL hay compared to WL hay(P〈0.05). Denatured gradient gel electrophoresis(DGGE) bands and richness index(S) were significantly affected by the interaction between F:C and FL(P〈0.05), under high F:C, band numbers and richness index from WL group were higher than that from SL group(P〈0.05), whereas there were no differences between WL andSL groups under low F:C(P〉0.05). Microflora similarity was 50–73% among the different treatments. It is concluded that the WL with high F:C(65:35) diet is suitable for weaned calves.
摘要The nutritive quality of guar (Cyamopsis tetragonoloba) residues (GR) was investigated and the effects of partial replacement of wheat straw by GR on nutrients intake, digestibility, and growth performance. Twenty four male lambs, with average body weight (BW) of 40.3 ± 2.5 kg were divided into three groups. Lambs were fed individually for 104 days with concentrate mixture (18% crude protein) and wheat straw as a total mixed ration. Wheat straw was replaced with 0, 100 and 145 g/kg dry matter guar residues. All rations were isonitrogenous and isocaloric. The fattening trial lasted 104 days. At the end of week 10 of the trail, a digestion trial was performed using six lambs from each group. The composition of nutrients in the GR was 87%, 8.5%, 56.4% and 40.1% for DM, CP, NDF and ADF, respectively. DM and crude protein (CP) intake were not affected by the inclusion of GR. However, neutral detergent fibre (NDF) and acid detergent fibre (ADF) intake were lower in lambs fed GR compared to control lambs. At the end of the experiment, lambs fed 100 and 145 g GR/kg DM diets gained more weight (P version (CR). Results from this work suggested that GR had advantages compared to regular roughage (wheat straw) in regard to parameters investigated as well as the significant reduction in fattening diets cost.
基金supported by the Key Research and Development Program of Sichuan Province(2023YFN0075)the Sichuan Rabbit Innovation Team of National Modern Agricultural Industry Technology System of China(SCCXTD-24)。
摘要Background Given the high incidence of gastrointestinal disorders in intensive rabbit production,we assessed the effects of graded levels of xylooligosaccharides(XOS)on growth performance,nutrient digestibility and intestinal health in growing rabbits.Methods The 35-day-old weaned rabbits(889.41±0.41 g)were randomly assigned to five dietary treatments(0,0.2,0.3,0.4 or 0.5 g/kg XOS)and the trial lasted for 35 d.Results The results revealed that linear trend responses of body weight(BW)to XOS on d 21 and 35(P≤0.05).During d 1-21,0.2 g/kg XOS increased average daily feed intake(ADFI)while 0.5 g/kg improved feed conversion ratio(FCR)significantly(P≤0.05).Weight gain rate(WGR)showed a linear trend,while FCR showed a quadratic response(P≤0.05).Throughout the 35-d trial,0.2 and 0.3 g/kg XOS enhanced ADFI,and 0.4 g/kg XOS improved FCR significantly,average daily gain(ADG)demonstrated linear dose-responsiveness,while WGR and FCR showed quadratic trends(P≤0.05).Notably,0.2 g/kg XOS elevated serum glutathione peroxidase(GSH-Px)activity and ileal secretory immunoglobulin A(sIgA)levels.Furthermore,0.3,0.4 and 0.5 g/kg XOS reduced jejunal malonaldehyde(MDA)content,0.4 g/kg XOS decreased serum MDA,and 0.5 g/kg XOS elevated serum immunoglobulin M(IgM)significantly(P≤0.05).0.2,0.4,0.5 g/kg XOS improved the digestibility of crude fiber(CF),0.2 and 0.4 g/kg XOS increased acid detergent fiber(ADF),and neutral detergent fiber(NDF)also increased among all treatments,although 0.5 g/kg XOS reduced cellulase activity significantly(P≤0.05).Furthermore,graded levels of XOS significantly changed the relative abundance of specific bacteria,and 0.4 and 0.5 g/kg XOS enhanced the content of valeric acid significantly(P≤0.05).Conclusions In conclusion,dietary supplementation of XOS serves as an effective nutritional strategy to optimize bacterial community in the cecum,improve fiber digestion and valeric acid production,while enhances resistance to intestinal pathogen infection and oxidative stress in rabbit production.