BACKGROUND Perianal abscess is an abscess caused by acute or chronic infection in the space surrounding the rectum and anal canal.If untreated or improperly managed,it may progress to a chronic infection and develop i...BACKGROUND Perianal abscess is an abscess caused by acute or chronic infection in the space surrounding the rectum and anal canal.If untreated or improperly managed,it may progress to a chronic infection and develop into an anal fistula,severely threatening patient health.AIM To investigate the therapeutic benefits of perianal infectious diseases by evaluating its bactericidal effects on pathogenic bacteria,its influence on tissue growth factor expression,and its macroscopic effects on tissue repair.METHODS Medical records of 65 patients with perianal abscess or anal fistula admitted to the Guihang Guiyang Hospital between January 2022 and May 2022 were retrospectively reviewed.The distribution of bacterial strains isolated from pus and secretion cultures collected pre-operatively and intra-operatively was analyzed,and the five most common pathogenic bacteria were identified.Ozonated water at varying concentrations was then applied to the cultured bacteria to evaluate bactericidal effects within the same exposure time and determine the minimum effective concentration(Cmin)capable of eliminating the major pathogenic bacteria associated with common perianal infectious diseases.Additionally,another patient cohort of 143 patients with perineal abscess or anal fistula treated between June 2022 and April 2024 was enrolled,including 79 patients in the observation group receiving ozone treatment and 64 patients in the control group receiving routine therapy.Both groups underwent surgical intervention.The control group additionally received routine anti-inflammatory therapy,sitz baths,and postoperative wound dressing changes.In addition to these treatments,the observation group received postoperative irrigation of the abscess cavity wound with ozonated water at concentration Cmin(100 mL,rinsed for 1 minute,once in the morning and once in the evening).After intervention,the expression levels of vascular endothelial growth factor,transforming growth factor-β,and platelet-derived growth factor in perianal wound tissue were assessed,and the wound healing outcomes were compared between groups.RESULTS Among the 65 patients,56 showed positive bacterial cultures from pyogenic fluids.The identified bacteria were categorized as follows:(1)Pathogenic bacteria:Escherichia coli(34/56),Klebsiella pneumoniae(11/56),Staphylococcus aureus(4/56),Enterococcus faecium(3/56),and Proteus mirabilis(2/56);and(2)Nonpathogenic bacteria:Miscellaneous bacteria,including normal skin flora(2/56).Under the same exposure time,ozonated water exerted stronger bactericidal effects with increasing concentration and achieved complete elimination of the above pathogenic bacteria within 1 minute at a Cmin of 2.5 mg/L.The observation group showed significantly higher expression levels of tissue growth factors than the controls after treatment(vascular endothelial growth factor:36.42±1.60 vs 25.85±1.18;transforming growth factor-β:15.81±2.04 vs 10.63±0.80;platelet-derived growth factor:33.32±1.48 vs 24.75±1.75;P<0.001).At one week after treatment,the observation group also demonstrated significantly reduced wound secretion(0.92±0.47 vs 1.27±0.54;P<0.001),tissue edema(0.54±0.50 vs 0.94±0.77;P<0.05),and postoperative pain(1.77±0.80 vs 2.94±1.10;P<0.001),along with significantly faster granulation tissue growth(0.67±0.65 vs 1.19±0.69;P<0.001)compared with the control group.CONCLUSION Ozonated water irrigation is an effective approach for managing infected wounds in patients with perianal abscess or anal fistula and demonstrates promising clinical application value.展开更多
BACKGROUND Gastro-thoracic fistula is a serious complication after radical surgery for esophageal cancer,and a conservative approach or endoscopic intervention is commonly applied to treat most cases.CASE SUMMARY Here...BACKGROUND Gastro-thoracic fistula is a serious complication after radical surgery for esophageal cancer,and a conservative approach or endoscopic intervention is commonly applied to treat most cases.CASE SUMMARY Here we describe the case of a patient with a gastro-thoracic fistula which could not be closed during gastroscopy after receiving postoperative radiotherapy,together with severe multiple drug-resistant bacterial infection and chest wall fistula.The abscess was drained and local irrigation applied with ozonated water,together with oral ozonated water,which achieved a good effect and highlighted a new way to cure fistula in such patients.CONCLUSION Patients with gastro-thoracic fistula that cannot be closed and severe infection can be treated by drainage and flushing with ozonated water.展开更多
The source water in one forest region of the Northeast China had very high natural organic matter(NOM) concentration and heavy color during snowmelt period. The efficiency of five combined treatment processes was co...The source water in one forest region of the Northeast China had very high natural organic matter(NOM) concentration and heavy color during snowmelt period. The efficiency of five combined treatment processes was compared to address the high concentration of NOM and the mechanisms were also analyzed. Conventional treatment can hardly remove dissolved organic carbon(DOC) in the source water. KMn O4pre-oxidization could improve the DOC removal to 22.0%. Post activated carbon adsorption improved the DOC removal of conventional treatment to 28.8%. The non-sufficient NOM removal could be attributed to the dominance of large molecular weight organic matters in raw water, which cannot be adsorbed by the micropore upon activated carbon. O3+ activated carbon treatment are another available technology for eliminating the color and UV254 in water. However, its performance of DOC removal was only 36.4%, which could not satisfy the requirement for organic matter. The limited ozone dosage is not sufficient to mineralize the high concentration of NOM. Magnetic ion-exchange resin combined with conventional treatment could remove 96.2%of color, 96.0% of UV254 and 87.1% of DOC, enabling effluents to meet the drinking water quality standard. The high removal efficiency could be explained by the negative charge on the surface of NOM which benefits the static adsorption of NOM on the anion exchange resin. The results indicated that magnetic ion-exchange resin combined with conventional treatment is the best available technology to remove high concentration of NOM.展开更多
Electric discharge in and in contact with water can accompany ultraviolet(UV)radiation and electron impact, which can generate a large number of active species such as hydroxyl radicals(OH), oxygen radical(O), o...Electric discharge in and in contact with water can accompany ultraviolet(UV)radiation and electron impact, which can generate a large number of active species such as hydroxyl radicals(OH), oxygen radical(O), ozone(O_3) and hydrogen peroxide(H_2O_2). In this paper, a nonthermal plasma processing system was established by means of dielectric barrier discharge(DBD)arrays in water mist spray. The relationship between droplet size and water content was examined,and the effects of the concentrations of oxides in both treated water and gas were investigated under different water content and discharge time. The relative intensity of UV spectra from DBD in water mist was a function of water content. The concentrations of both O_3 and nitrogen dioxide(NO_2) in DBD room decreased with increasing water content. Moreover, the concentrations of H_2O_2, O_3 and nitrogen oxides(NOx) in treated water decreased with increasing water content,and all the ones enhanced after discharge. The experimental results were further analyzed by chemical reaction equations and commented by physical principles as much as possible. At last,the water containing phenol was tested in this system for the concentration from 100 mg/L to9.8 mg/L in a period of 35 min.展开更多
We report the catalytic properties of ultra-small β-FeOOH nanorods in ozonation of4-chlorophenol(4-CP). XRD, TEM, EDS, SAED, FTIR and BET were used to characterize the prepared material. Interaction between O3 and ...We report the catalytic properties of ultra-small β-FeOOH nanorods in ozonation of4-chlorophenol(4-CP). XRD, TEM, EDS, SAED, FTIR and BET were used to characterize the prepared material. Interaction between O3 and β-FeOOH was evident from the FTIR spectra.The removal efficiency of 4-CP was significantly enhanced in the presence of β-FeOOH compared to ozone alone. Removal efficiency of 99% and 67% was achieved after 40 min in the presence of combined ozone and catalyst and ozone only, respectively. Increasing catalyst load increased COD removal efficiency. Maximum COD removal of 97% was achieved using a catalyst load of 0.1 g/100 m L of 4-CP solution. Initial 4-CP concentration was not found to be rate limiting below 2 × 10^-3mol/L. The catalytic properties of the material during ozonation process were found to be pronounced at lower initial p H of 3.5.Two stage first order kinetics was applied to describe the kinetic behavior of the nanorods at low p H. The first stage of catalytic ozonation was attributed to the heterogeneous surface breakdown of O3 by β-FeOOH, while the second stage was attributed to homogeneous catalysis initiated by reductive dissolution of β-FeOOH at low p H.展开更多
Effects of ozonated water as sanitizer method on mango was studied on total phenolics, flavonoids, carotenoids and vitamin C of pulp or peel. Mango cultivar “Palmer” was harvested and subjected to sanitization treat...Effects of ozonated water as sanitizer method on mango was studied on total phenolics, flavonoids, carotenoids and vitamin C of pulp or peel. Mango cultivar “Palmer” was harvested and subjected to sanitization treatments by immersion in water, chlorinated water (10 minutes sodium hypochlorite 100 mg·L-1) or ozonated water for 10 and 20 minutes. After the sanitization process, the mangoes were stored at 15°C ± 1°C and 85% ± 5% RH for seven days, followed by 4 days of storage at room temperature (simulating the trading period), totaling 11 days after harvested. Mangoes pulp sanitized with ozonated water for 20 minutes showed the highest values of TA, total soluble carbohydrates, vitamin C, carotenoids and flavonoid content. These data suggest that the use of ozonated water may contribute to induce increase antioxidants compounds.展开更多
Ozonated water(4 ppm)has been used to sanitize red grapes(cv Tempranillo,Vitis vinifera L.)after destemming and to facilitate the implantation of the non-Saccharomyces yeast Lachancea thermotolerans in the absence of ...Ozonated water(4 ppm)has been used to sanitize red grapes(cv Tempranillo,Vitis vinifera L.)after destemming and to facilitate the implantation of the non-Saccharomyces yeast Lachancea thermotolerans in the absence of sulphites.The implantation of L.thermotolerans in ozone fermentations was more successful with a higher acidification(+13%of lactic acid)and a lower variability among replicates(n=3).Ozone fermentations kept the same amount of anthocyanins(p<0.05)measured by HPLC-DAD and lower values of acetaldehyde and ethyl acetate analyzed by GC-FID.The concentration of higher alcohols,esters and carbonyl compounds was similar in ozone fermentations and controls(p<0.05).Ozonated water is a powerful tool to control microorganisms facilitating fermentations with non-Saccharomyces sensitive to sulfites like Lachancea thermotolerans and the wine making with low levels of SO2.The better implantation produces more reproducible fermentations in terms of volatile compounds and metabolites.展开更多
The application of aqueous solutions of ozone for surface disinfection is an effective novel green technology with a potential for replacing the use of persistent chemicals in postharvest treatments. However, successf...The application of aqueous solutions of ozone for surface disinfection is an effective novel green technology with a potential for replacing the use of persistent chemicals in postharvest treatments. However, successful disinfection requires certain levels of ozone to be maintained throughout the process. The decay rates of aqueous ozone were found to vary with the presence of different fruit and vegetables commodities (apples, carrots, onions, celeriac, and pears). Pure aqueous ozone had a half-life of 3200 s, whereas the half-life of ozone was found to range with increasing cross-cut areas between 2177 and 291 s for apples, 573 and 345 s for carrots, 541 and 113 s for onions, 2800 and 253 s for pears, and 362 and 165 s for celeriac. With soil particles present, the ozone half-life dropped to 59 s for celeriac. Parallel measurements reported strong to moderate effect of soil particles (51–626 s, 10 g soil/L ozonated water), and naturally occurring fungi (850–2294 s, 0.25 g fungi mix/L ozonated water) on ozone half-life. In summary, presence of organic compounds, notably by damaged commodities, increase ozone decay and illustrate the need to correctly identify important ozone-depleting parameters, which is crucial for understanding the efficiency of ozone-based washing systems.展开更多
基金Supported by General Medical 2022 Annual Research Fund Project,No.TYYLKYJJ-2022-034.
摘要BACKGROUND Perianal abscess is an abscess caused by acute or chronic infection in the space surrounding the rectum and anal canal.If untreated or improperly managed,it may progress to a chronic infection and develop into an anal fistula,severely threatening patient health.AIM To investigate the therapeutic benefits of perianal infectious diseases by evaluating its bactericidal effects on pathogenic bacteria,its influence on tissue growth factor expression,and its macroscopic effects on tissue repair.METHODS Medical records of 65 patients with perianal abscess or anal fistula admitted to the Guihang Guiyang Hospital between January 2022 and May 2022 were retrospectively reviewed.The distribution of bacterial strains isolated from pus and secretion cultures collected pre-operatively and intra-operatively was analyzed,and the five most common pathogenic bacteria were identified.Ozonated water at varying concentrations was then applied to the cultured bacteria to evaluate bactericidal effects within the same exposure time and determine the minimum effective concentration(Cmin)capable of eliminating the major pathogenic bacteria associated with common perianal infectious diseases.Additionally,another patient cohort of 143 patients with perineal abscess or anal fistula treated between June 2022 and April 2024 was enrolled,including 79 patients in the observation group receiving ozone treatment and 64 patients in the control group receiving routine therapy.Both groups underwent surgical intervention.The control group additionally received routine anti-inflammatory therapy,sitz baths,and postoperative wound dressing changes.In addition to these treatments,the observation group received postoperative irrigation of the abscess cavity wound with ozonated water at concentration Cmin(100 mL,rinsed for 1 minute,once in the morning and once in the evening).After intervention,the expression levels of vascular endothelial growth factor,transforming growth factor-β,and platelet-derived growth factor in perianal wound tissue were assessed,and the wound healing outcomes were compared between groups.RESULTS Among the 65 patients,56 showed positive bacterial cultures from pyogenic fluids.The identified bacteria were categorized as follows:(1)Pathogenic bacteria:Escherichia coli(34/56),Klebsiella pneumoniae(11/56),Staphylococcus aureus(4/56),Enterococcus faecium(3/56),and Proteus mirabilis(2/56);and(2)Nonpathogenic bacteria:Miscellaneous bacteria,including normal skin flora(2/56).Under the same exposure time,ozonated water exerted stronger bactericidal effects with increasing concentration and achieved complete elimination of the above pathogenic bacteria within 1 minute at a Cmin of 2.5 mg/L.The observation group showed significantly higher expression levels of tissue growth factors than the controls after treatment(vascular endothelial growth factor:36.42±1.60 vs 25.85±1.18;transforming growth factor-β:15.81±2.04 vs 10.63±0.80;platelet-derived growth factor:33.32±1.48 vs 24.75±1.75;P<0.001).At one week after treatment,the observation group also demonstrated significantly reduced wound secretion(0.92±0.47 vs 1.27±0.54;P<0.001),tissue edema(0.54±0.50 vs 0.94±0.77;P<0.05),and postoperative pain(1.77±0.80 vs 2.94±1.10;P<0.001),along with significantly faster granulation tissue growth(0.67±0.65 vs 1.19±0.69;P<0.001)compared with the control group.CONCLUSION Ozonated water irrigation is an effective approach for managing infected wounds in patients with perianal abscess or anal fistula and demonstrates promising clinical application value.
基金Foundation of President of Nanfang Hospital,Southern Medical University.
摘要BACKGROUND Gastro-thoracic fistula is a serious complication after radical surgery for esophageal cancer,and a conservative approach or endoscopic intervention is commonly applied to treat most cases.CASE SUMMARY Here we describe the case of a patient with a gastro-thoracic fistula which could not be closed during gastroscopy after receiving postoperative radiotherapy,together with severe multiple drug-resistant bacterial infection and chest wall fistula.The abscess was drained and local irrigation applied with ozonated water,together with oral ozonated water,which achieved a good effect and highlighted a new way to cure fistula in such patients.CONCLUSION Patients with gastro-thoracic fistula that cannot be closed and severe infection can be treated by drainage and flushing with ozonated water.
基金supported by the project of " Major Science and Technology Program for Water Pollution Control and Treatment of China " (No. 2008ZX07420-005)the Natural Science Foundation of China (No. 51290284)the Tsinghua University Initiative Scientific Research Program (No. 20131089247)
摘要The source water in one forest region of the Northeast China had very high natural organic matter(NOM) concentration and heavy color during snowmelt period. The efficiency of five combined treatment processes was compared to address the high concentration of NOM and the mechanisms were also analyzed. Conventional treatment can hardly remove dissolved organic carbon(DOC) in the source water. KMn O4pre-oxidization could improve the DOC removal to 22.0%. Post activated carbon adsorption improved the DOC removal of conventional treatment to 28.8%. The non-sufficient NOM removal could be attributed to the dominance of large molecular weight organic matters in raw water, which cannot be adsorbed by the micropore upon activated carbon. O3+ activated carbon treatment are another available technology for eliminating the color and UV254 in water. However, its performance of DOC removal was only 36.4%, which could not satisfy the requirement for organic matter. The limited ozone dosage is not sufficient to mineralize the high concentration of NOM. Magnetic ion-exchange resin combined with conventional treatment could remove 96.2%of color, 96.0% of UV254 and 87.1% of DOC, enabling effluents to meet the drinking water quality standard. The high removal efficiency could be explained by the negative charge on the surface of NOM which benefits the static adsorption of NOM on the anion exchange resin. The results indicated that magnetic ion-exchange resin combined with conventional treatment is the best available technology to remove high concentration of NOM.
基金supported by National Natural Science Foundation of China(Nos.11274092,51107033,11404092,11274091)the Nantong Science and Technology Project,China(No.BK2014024)+1 种基金the Open Project of Jiangsu Province Key Laboratory of Environmental Engineering,China(No.KF2014001)the Fundamental Research Funds for the Central Universities,China(No.2014B11414)
摘要Electric discharge in and in contact with water can accompany ultraviolet(UV)radiation and electron impact, which can generate a large number of active species such as hydroxyl radicals(OH), oxygen radical(O), ozone(O_3) and hydrogen peroxide(H_2O_2). In this paper, a nonthermal plasma processing system was established by means of dielectric barrier discharge(DBD)arrays in water mist spray. The relationship between droplet size and water content was examined,and the effects of the concentrations of oxides in both treated water and gas were investigated under different water content and discharge time. The relative intensity of UV spectra from DBD in water mist was a function of water content. The concentrations of both O_3 and nitrogen dioxide(NO_2) in DBD room decreased with increasing water content. Moreover, the concentrations of H_2O_2, O_3 and nitrogen oxides(NOx) in treated water decreased with increasing water content,and all the ones enhanced after discharge. The experimental results were further analyzed by chemical reaction equations and commented by physical principles as much as possible. At last,the water containing phenol was tested in this system for the concentration from 100 mg/L to9.8 mg/L in a period of 35 min.
基金supported by the National Research Foundation of South Africa (No: 88220)the Cape Peninsula University of Technology (University Research Funding) (URF:2014)
摘要We report the catalytic properties of ultra-small β-FeOOH nanorods in ozonation of4-chlorophenol(4-CP). XRD, TEM, EDS, SAED, FTIR and BET were used to characterize the prepared material. Interaction between O3 and β-FeOOH was evident from the FTIR spectra.The removal efficiency of 4-CP was significantly enhanced in the presence of β-FeOOH compared to ozone alone. Removal efficiency of 99% and 67% was achieved after 40 min in the presence of combined ozone and catalyst and ozone only, respectively. Increasing catalyst load increased COD removal efficiency. Maximum COD removal of 97% was achieved using a catalyst load of 0.1 g/100 m L of 4-CP solution. Initial 4-CP concentration was not found to be rate limiting below 2 × 10^-3mol/L. The catalytic properties of the material during ozonation process were found to be pronounced at lower initial p H of 3.5.Two stage first order kinetics was applied to describe the kinetic behavior of the nanorods at low p H. The first stage of catalytic ozonation was attributed to the heterogeneous surface breakdown of O3 by β-FeOOH, while the second stage was attributed to homogeneous catalysis initiated by reductive dissolution of β-FeOOH at low p H.
基金thank FAPESP(Sao Paulo Research Foundation)for grating(2013/05644-3)CNPq(National Council for Scientific and Technological Development)(478372/2013-2 and 141354/2012-7)CAPES(Coordination of Improvement of Higher Level Personnel)(2441/09-8 and 478375/2010-7).
摘要Effects of ozonated water as sanitizer method on mango was studied on total phenolics, flavonoids, carotenoids and vitamin C of pulp or peel. Mango cultivar “Palmer” was harvested and subjected to sanitization treatments by immersion in water, chlorinated water (10 minutes sodium hypochlorite 100 mg·L-1) or ozonated water for 10 and 20 minutes. After the sanitization process, the mangoes were stored at 15°C ± 1°C and 85% ± 5% RH for seven days, followed by 4 days of storage at room temperature (simulating the trading period), totaling 11 days after harvested. Mangoes pulp sanitized with ozonated water for 20 minutes showed the highest values of TA, total soluble carbohydrates, vitamin C, carotenoids and flavonoid content. These data suggest that the use of ozonated water may contribute to induce increase antioxidants compounds.
基金Project“Optimización del proceso de fermentación mediante pretratamiento de la materia prima y la mejora de los protocolos y con-diciones de generación de biomasa”(PAG-020100-2023-274-PP09),Plan de Recuperación Transformación y Resiliencia.NextGenerationEU funding.Ministerio de Industria,Comercio y Turismo.
摘要Ozonated water(4 ppm)has been used to sanitize red grapes(cv Tempranillo,Vitis vinifera L.)after destemming and to facilitate the implantation of the non-Saccharomyces yeast Lachancea thermotolerans in the absence of sulphites.The implantation of L.thermotolerans in ozone fermentations was more successful with a higher acidification(+13%of lactic acid)and a lower variability among replicates(n=3).Ozone fermentations kept the same amount of anthocyanins(p<0.05)measured by HPLC-DAD and lower values of acetaldehyde and ethyl acetate analyzed by GC-FID.The concentration of higher alcohols,esters and carbonyl compounds was similar in ozone fermentations and controls(p<0.05).Ozonated water is a powerful tool to control microorganisms facilitating fermentations with non-Saccharomyces sensitive to sulfites like Lachancea thermotolerans and the wine making with low levels of SO2.The better implantation produces more reproducible fermentations in terms of volatile compounds and metabolites.
基金a part of the project WASHO3 financed by the Green Development and Demonstration Programme(GUDP)in Denmark(J.no.:34009-18-1366).
摘要The application of aqueous solutions of ozone for surface disinfection is an effective novel green technology with a potential for replacing the use of persistent chemicals in postharvest treatments. However, successful disinfection requires certain levels of ozone to be maintained throughout the process. The decay rates of aqueous ozone were found to vary with the presence of different fruit and vegetables commodities (apples, carrots, onions, celeriac, and pears). Pure aqueous ozone had a half-life of 3200 s, whereas the half-life of ozone was found to range with increasing cross-cut areas between 2177 and 291 s for apples, 573 and 345 s for carrots, 541 and 113 s for onions, 2800 and 253 s for pears, and 362 and 165 s for celeriac. With soil particles present, the ozone half-life dropped to 59 s for celeriac. Parallel measurements reported strong to moderate effect of soil particles (51–626 s, 10 g soil/L ozonated water), and naturally occurring fungi (850–2294 s, 0.25 g fungi mix/L ozonated water) on ozone half-life. In summary, presence of organic compounds, notably by damaged commodities, increase ozone decay and illustrate the need to correctly identify important ozone-depleting parameters, which is crucial for understanding the efficiency of ozone-based washing systems.