Field experiments were carried out with rice variety of Shennong 91 of short culms and erect panicles to study nutrient contents in high-yielding rice plants and to increase rice yield by appropriate fertilization.Nit...Field experiments were carried out with rice variety of Shennong 91 of short culms and erect panicles to study nutrient contents in high-yielding rice plants and to increase rice yield by appropriate fertilization.Nitrogen, phosphorus, potassium, magnesium, zinc, manganese, iron and copper contents in rice plants varied with different treatment factors. The relationship between the nutrient contents and treatment factors could be simulated using a multiple quadratic equation. The nutrient contents in plants should be appropriate for high-yielding rice. If the mean nutrient content in rice plants producing 11 t ha-1 or more of grain (μj) was set as the standard value and the standard deviation (σj) was set as the range of variation, the nutrient content in high-yielding rice plants should be μj±1.99σj. Rice leaves were sensitive to the nutrient elements. Heavy nitrogen dressing increased the content of nitrogen in rice plants. Sparse transplanting also increased nitrogen content. Improper application of nitrogen, phosphorus and potassium could affect the nutrient contents and decrease the grain yield.展开更多
Detection of crop health conditions plays an important role in making control strategies of crop disease and insect damage and gaining high-quality production at late growth stages. In this study, hyperspectral reflec...Detection of crop health conditions plays an important role in making control strategies of crop disease and insect damage and gaining high-quality production at late growth stages. In this study, hyperspectral reflectance of rice panicles was measured at the visible and near-infrared regions. The panicles were divided into three groups according to health conditions: healthy panicles, empty panicles caused by Nilaparvata lugens St^l, and panicles infected with Ustilaginoidea virens. Low order derivative spectra, namely, the first and second orders, were obtained using different techniques. Principal component analysis (PCA) was performed to obtain the principal component spectra (PCS) of the foregoing derivative and raw spectra to reduce the reflectance spectral dimension. Support vector classification (SVC) was employed to discriminate the healthy, empty, and infected panicles, with the front three PCS as the in- dependent variables. The overall accuracy and kappa coefficient were used to assess the classification accuracy of SVC. The overall accuracies of SVC with PCS derived from the raw, first, and second reflectance spectra for the testing dataset were 96.55%, 99.14%, and 96.55%, and the kappa coefficients were 94.81%, 98.71%, and 94.82%, respectively. Our results demonstrated that it is feasible to use visible and near-infrared spectroscopy to discriminate health conditions of rice panicles.展开更多
The abnormal rice with small grains and erect panicles were found on a large scale in China, which showed shortend rice panicle and decreased number of grains in comparison with normal rice, and the grain was small an...The abnormal rice with small grains and erect panicles were found on a large scale in China, which showed shortend rice panicle and decreased number of grains in comparison with normal rice, and the grain was small and black-brown, and some of them were distorted, while the flag leaf was normal. A kind of nematode of Aphelenchoides was isolated from the grains of rice variety Wuyujing 3 which performed 'small grains and erect panicles' symptom. There were 2014 nematodes in one hundred grains infected, and up to 74 in single grain, 92 percent of the grains tested had nematodes in the infested panicles. The diagnosis characters of nematode include lateral fields about one-fourth as wide as body, with 4 incisures. The terminus bears a mucro of diverse shape with 3-4 pointed processes. The female post-vulval uterine sac extends less than 50% of distance from vulva to anus, no sperm in it. Oocytes usually arrange in 2-4 rows. The male spicules have a moderately developed rostrum. Morphological measurements showed it to be conspecific with Aphelenchoides besseyi Christie, 1942.展开更多
Self-occlusions are common in rice canopy images and strongly influence the calculation accuracies of panicle traits. Such interference can be largely eliminated if panicles are phenotyped at the 3 D level.Research on...Self-occlusions are common in rice canopy images and strongly influence the calculation accuracies of panicle traits. Such interference can be largely eliminated if panicles are phenotyped at the 3 D level.Research on 3 D panicle phenotyping has been limited. Given that existing 3 D modeling techniques do not focus on specified parts of a target object, an efficient method for panicle modeling of large numbers of rice plants is lacking. This paper presents an automatic and nondestructive method for 3 D panicle modeling. The proposed method integrates shoot rice reconstruction with shape from silhouette, 2 D panicle segmentation with a deep convolutional neural network, and 3 D panicle segmentation with ray tracing and supervoxel clustering. A multiview imaging system was built to acquire image sequences of rice canopies with an efficiency of approximately 4 min per rice plant. The execution time of panicle modeling per rice plant using 90 images was approximately 26 min. The outputs of the algorithm for a single rice plant are a shoot rice model, surface shoot rice model, panicle model, and surface panicle model, all represented by a list of spatial coordinates. The efficiency and performance were evaluated and compared with the classical structure-from-motion algorithm. The results demonstrated that the proposed method is well qualified to recover the 3 D shapes of rice panicles from multiview images and is readily adaptable to rice plants of diverse accessions and growth stages. The proposed algorithm is superior to the structure-from-motion method in terms of texture preservation and computational efficiency. The sample images and implementation of the algorithm are available online. This automatic, cost-efficient, and nondestructive method of 3 D panicle modeling may be applied to high-throughput 3 D phenotyping of large rice populations.展开更多
Under field conditions, three erect panicle type japonica dee cultivars and three curved panicle type japonica rice cultivars were applied as experimental materials to investigate the differences of protein compositio...Under field conditions, three erect panicle type japonica dee cultivars and three curved panicle type japonica rice cultivars were applied as experimental materials to investigate the differences of protein composition of grains at different positions in panicles between two different panicle types of japonica rice. Accord- ing to the results, the panicle type of japonica rice had no direct correlation with albumin content, globulin content, prolamin content and glutelin content in rice, variations of protein composition of grains at different positions in panicles. There were certain correlations between soluble protein contents at different grain posi- tions in the same panicle and the flowering order of glumous flowers in the panicle. Albumin content, prolamin content and glutelin content in grains on secondary rachis branches of two panicle types (erect and curved) of japonica rice cuhivars were higher than that on primary rachis branches, while globulin content exhibited an opposite trend. Globulin content, prolamin content and glutelin content in grains at different positions demonstrated a descending order of bottom 〉 middle 〉 top, while albumin content exhibited an opposite trend. The interactions between primary and secondary rachis branches and among top, middle and bottom rachis branches significantly affected soluble protein contents.展开更多
Panicles of an indica rice line TM7-5 were subjected to radiation with 137^Cs gamma rays at 0 (control), 5, 10, 15 and 20 Gy respectively, and then its anthers were cultured. There were slight differences among the ...Panicles of an indica rice line TM7-5 were subjected to radiation with 137^Cs gamma rays at 0 (control), 5, 10, 15 and 20 Gy respectively, and then its anthers were cultured. There were slight differences among the treatments in peak emerging time of callus initiation, from 38 to 44 days after inoculation (DAI) as well as the frequency of callus initiation (2.3-3.5%). About two thirds calli were induced before 44 DAI, and calli derived beyond 60 DAI lost the regeneration ability. Green plant regeneration frequency was significantly stimulated from two- to three-fold by irradiation of the 1370S gamma rays compared with the control, and the maximum was 22,81% (15 Gy). The culture ability based on callus initiation and green plantlet regeneration was 0.19% for the control while it was over 0.45% for all the irradiated treatments, and the maximum was 0,59% for 15 Gy treatment. The advantages of panicle irradiation before anther culture and the potential application in rice anther culture, especially for recalcitrant indica rice, were discussed.展开更多
Highlights Improving spikelet production efficiency(SPE)can further enhance the grain yield and harvest index in rice.Higher SPE can enhance post-anthesis photoassimilate production in the shoots of rice.Higher SPE ca...Highlights Improving spikelet production efficiency(SPE)can further enhance the grain yield and harvest index in rice.Higher SPE can enhance post-anthesis photoassimilate production in the shoots of rice.Higher SPE can promote the post-anthesis remobilization of non-structural carbohydrate(NSC)from rice stems.Rice grain yield is a complex agronomic trait determined by four key components:panicles per plant,spikelets per panicle,filled-grain rate,and grain weight.展开更多
Fungal endophytes are suggested to manipulate host grass reproduction.Using different grass-endophyte combinations in a four year common garden experiment,we show that endophyte can retain control over the reproductiv...Fungal endophytes are suggested to manipulate host grass reproduction.Using different grass-endophyte combinations in a four year common garden experiment,we show that endophyte can retain control over the reproductive functions of the host.Plants were collected as seeds from two different environments,meadows and riverbanks.Natural endophyte infection(E+)increased reproductive effort of plants;13 and 15%higher proportion of E+plants produced panicles compared to naturally uninfected(E-)and manipulatively endophyte-infected(ME+)plants,respectively.Meadoworigin E+plants produced also higher number of panicles compared to E-in the last two years,and both meadow-and riverbank-origin ME+plants produced higher number of panicles compared to ME-plants in most of the years.Pseudovivipary was recorded in 5%of the plants each year.Pollen limitation appears not to induce pseudovivipary.Both E+and ME+plants produced higher number of seminiferous panicles compared to E-and ME-plants.The higher proportion of pseudoviviparism in ME+plants compared to E+,Eand ME-plants from meadows suggests specific genotypegenotype cross-talk between endophyte and grass.Pseudovivipary was affected by year and primarily explained by plant reproductive effort since most vigorous plants produced more pseudoviviparous panicles.We propose that endophytes can promote host grass reproduction in a habitatspecific manner and depending on genetic compatibility between partners.However,reproductive behavior depended on maternal habitat and the annual environmental conditions highlighting the importance of long-term experiments to estimate the effects of endophytes on host plant ecology.展开更多
Rice blast is one of the most devastating diseases,particularly panicle blast leads to significant yield losses.Utilization of panicle blast resistance genes is one of the effective ways to control this disease.Howeve...Rice blast is one of the most devastating diseases,particularly panicle blast leads to significant yield losses.Utilization of panicle blast resistance genes is one of the effective ways to control this disease.However,genetic resources with panicle blast resistance are rare.The objective of this study was to identify the role of OsEXOⅢ-1,a previously identified candidate gene,in Pigm-mediated panicle blast resistance in rice.There were two main haplotypes in rice germplasm,with OsEXOⅢ-1~(Hap1),the product of the resistant haplotype,binding more strongly to PiBP1 than OsEXOⅢ-1~(Hap2).OsEXOⅢ-1/PiBP1 positively regulates Pigm-mediated panicle blast resistance by regulating expression of PR1 subfamily genes.OsEXOⅢcould be a target for breeding panicle blast-resistant cultivars.展开更多
Rice false smut,caused by the fungal pathogen Ustilaginoidea virens,is a notorious panicle disease.It significantly compromises grain yield and quality and results in the contamination of rice with mycotoxins,thereby ...Rice false smut,caused by the fungal pathogen Ustilaginoidea virens,is a notorious panicle disease.It significantly compromises grain yield and quality and results in the contamination of rice with mycotoxins,thereby posing significant risks to both human and animal health(Yu et al.,2023).Given the current limited availability of genetic resistance resources to combat this pathogen,identifying susceptibility factors in rice is a critical prerequisite for developing durable and effective disease management strategies(Huang et al.,2024).展开更多
Panicle number per plant,grain number per panicle,and grain weight are three key factors influencing rice grain yield.Gn1a,a major QTL for grain number per panicle,encodes the cytokinin oxidase/dehydrogenase(CKX)OsCKX...Panicle number per plant,grain number per panicle,and grain weight are three key factors influencing rice grain yield.Gn1a,a major QTL for grain number per panicle,encodes the cytokinin oxidase/dehydrogenase(CKX)OsCKX2.While the use of elite Gn1a alleles has been well documented in indica rice cultivars,their potential in japonica rice remains largely unexplored.In this study,we characterized three suppressor mutants of the rice cytokinin receptor mutant pal1/ohk4 and found that all causal genes were novel alleles of Gn1a identified through the MutMap approach.These three suppressor mutants caused single amino acid substitutions in the FAD-binding domain(G556D and G156D)and the cytokinin-binding domain(Y357C),resulting in significantly reduced enzymatic activity of OsCKX2 and elevated cytokinin levels in the panicle.Haplotype analysis of Gn1a using a natural population from the 3K Rice Genomes Project showed that G556D,G156D,and Y357C were novel alleles of Gn1a.G556,G156,and Y357 were highly conserved,whereas four natural variants G54A,A105V,H116R,and N535K identified in different haplotypes of Gn1a showed extremely low conservation.By backcrossing the suppressor mutants with their original wild-type Huaidao 5,an elite japonica rice variety,we developed improved lines carrying only the gn1a mutation.The improved lines showed a significant increase in grain number per panicle,grain weight,panicle number per plant,plant height,and stem thickness,leading to a 25.7%-28.7%increase in grain yield per plot compared with Huaidao 5.This study provides valuable Gn1a alleles for synergistic improvement of the three key yield factors and offers germplasm resources for high-yielding breeding in japonica rice.展开更多
High-throughput phenotyping is the bottleneck for advancing field trait characterization and yield improvement in major field crops.Specifically for sorghum(Sorghum bicolor L.),rapid plant-level yield estimation is hi...High-throughput phenotyping is the bottleneck for advancing field trait characterization and yield improvement in major field crops.Specifically for sorghum(Sorghum bicolor L.),rapid plant-level yield estimation is highly dependent on characterizing the number of grains within a panicle.In this context,the integration of computer vision and artificial intelligence algorithms with traditional field phenotyping can be a critical solution to reduce labor costs and time.Therefore,this study aims to improve sorghum panicle detection and grain number estimation from smartphone-capture images under field conditions.A preharvest benchmark dataset was collected at field scale(2023 season,Kansas,USA),with 648 images of sorghum panicles retrieved via smartphone device,and grain number counted.Each sorghum panicle image was manually labeled,and the images were augmented.Two models were trained using the Detectron2 and Yolov8 frameworks for detection and segmentation,with an average precision of 75%and 89%,respectively.For the grain number,3 models were trained:MCNN(multiscale convolutional neural network),TCNN-Seed(two-column CNN-Seed),and Sorghum-Net(developed in this study).The Sorghum-Net model showed a mean absolute percentage error of 17%,surpassing the other models.Lastly,a simple equation was presented to relate the count from the model(using images from only one side of the panicle)to the field-derived observed number of grains per sorghum panicle.The resulting framework obtained an estimation of grain number with a 17%error.The proposed framework lays the foundation for the development of a more robust application to estimate sorghum yield using images from a smartphone at the plant level.展开更多
Branching is a critical aspect of plant architecture that significantly impacts the yield and adaptability of staple cereal crops like rice and wheat.Cereal crops develop tillers during the vegetative stage and panicl...Branching is a critical aspect of plant architecture that significantly impacts the yield and adaptability of staple cereal crops like rice and wheat.Cereal crops develop tillers during the vegetative stage and panicle or spike branches during the reproductive stage,respectively,both of which are significantly impacted by hormones and genetic factors.Tillering and panicle branching are closely interconnected and exhibit high environmental plasticity.Here,we summarize the recent progress in genetic,hormonal,and environmental factors regulation in the branching of rice and wheat.This review not only provides a comprehensive overview of the current knowledge on branching mechanisms in rice and wheat,but also explores the prospects for future research aimed at optimizing crop architecture for enhanced productivity.展开更多
This study aimed to clarify the pathogen composition,biological characteristics,infection patterns,and effective control agents for panicle blight of rice in Heilongjiang Province.Diseased panicles were collected from...This study aimed to clarify the pathogen composition,biological characteristics,infection patterns,and effective control agents for panicle blight of rice in Heilongjiang Province.Diseased panicles were collected from different rice-growing areas in Heilongjiang Province and subjected to tissue isolation,pathogenicity tests,morphological observation,and molecular identification.The primary pathogens identified were Fusarium graminearum,Alternaria alternata and Nigrospora oryzae.The biological characteristics of these three pathogens were systematically investigated.Pathogenicity assays revealed that F.graminearum exhibited the strongest pathogenicity,followed by A.alternata,while N.oryzae was the weakest.In vitro toxicity tests screened highly effective fungicides:75%trifloxystrobin-tebuconazole showed the best inhibitory effect against F.graminearum(EC50=0.0140μg·mL-1);30%tebuconazole-azoxystrobin was the most effective against A.alternata(EC50=0.0060μg·mL-1)and N.oryzae(EC50=0.0310μg·mL-1).展开更多
Grain filling is a critical determinant of yield and quality in rice.This study aims to clarify the association between grain photosynthesis and the filling rate of rice varieties with different grain weights,providin...Grain filling is a critical determinant of yield and quality in rice.This study aims to clarify the association between grain photosynthesis and the filling rate of rice varieties with different grain weights,providing a theoretical foundation for optimizing grain-filling processes.Two rice varieties with similar growth duration but different grain weights were selected:a large-grain variety,Lingliangyou 268(L268),and a small-grain variety,Ruiliangyou 1053(R1053).Differences in grain filling,grain photosynthetic rate,and grain chlorophyll content were systematically examined during the filling stage.Results showed significant differences in grain-filling,grain photosynthetic rate,and grain chlorophyll content between large-grain and small-grain rice varieties.The grain photosynthetic rate of L268 was a significantly higher than R1053.L268 also exhibited significantly higher initial grain filling rate,maximum grainfilling rate,and mean grain filling rate compared to R1053.Throughout the grain filling period,L268 showed higher grain chlorophyll content(including chlorophyll a,chlorophyll b,and total chlorophyll)than R1053.The increase in chlorophyll content,particularly total chlorophyll,enhanced the grain photosynthetic rate during the early and middle stages of grain filling significantly.These findings suggested that rice varieties with higher grain weights exhibited stronger panicle photosynthetic capacity due to their higher chlorophyll content.The enhanced grain photosynthetic rate contributed to improved grain filling and increased grain weight.展开更多
Rice grain yield is primarily determined by three key agronomic traits:panicle number,grain number per panicle,and grain weight(GW).However,the inherent tradeoffs among these yield components remain a persistent chall...Rice grain yield is primarily determined by three key agronomic traits:panicle number,grain number per panicle,and grain weight(GW).However,the inherent tradeoffs among these yield components remain a persistent challenge in rice breeding programs.Notably,compared with GW,brown rice weight(BRW)provides a more direct metric associated with actual grain yield potential.In this study,we conducted a two-year replicated genome-wide association study to elucidate the genetic architecture of BRW and identify new loci regulating GW.Among seven consistently detected loci across experimental replicates,four were not co-localized with previously reported genes associated with BRW or GW traits.BRW1.1,one of the four newly identified loci,was found to encode a novel RNA-binding protein.Functional characterization revealed that BRW1.1 acts as a negative regulator of BRW,potentially through modulating mRNA translation processes.Intriguingly,through integrated analysis of mutant phenotypes and haplotype variations,we demonstrated that BRW1.1 mediates the physiological tradeoff between GW and panicle number.This study not only delineates the genetic determinants of BRW but also identifies BRW1.1 as a promising molecular target for breaking the yield component tradeoff in precision rice breeding.展开更多
In the last several decades, Particle Image Velocimetry(PIV) has reached a high degree of maturity as a laser diagnostic technique based on tracer particles, with significant improvements in accuracy, resolution, dy...In the last several decades, Particle Image Velocimetry(PIV) has reached a high degree of maturity as a laser diagnostic technique based on tracer particles, with significant improvements in accuracy, resolution, dynamic range, and as an extension to combustion measurements. To assess the recent developments and to project the future trends of using the PIV technique for combustion measurements, we review many key issues for measuring combustion flow fields. We introduce the representative applications of a supersonic combustor and swirling burner and summarize the promising prospects and further development requirements of PIV measurements in combustion flow fields.展开更多
[Objective]This study aimed to reveal the effects of Aphelenchoides besseyi infection on different rice varieties(lines).[Method]By field observation and indoor phenotypic investigation,four conventional japonica rice...[Objective]This study aimed to reveal the effects of Aphelenchoides besseyi infection on different rice varieties(lines).[Method]By field observation and indoor phenotypic investigation,four conventional japonica rice varieties(lines)and japonica rice restorer line R161 under natural onset conditions were observed and analyzed.[Result]After being infected by A.besseyi,different rice varieties(lines)exhibited various symptoms.Specifically,Ning 1707,Ning 1818,Zhendao 88 and Nanjing 9108 had withered leaf tips and exhibited the symptoms of"small grains and erect panicles";japonica rice restorer line R161 only had withered leaf tips without symptoms of"small grains and erect panicles",and the withering symptoms occurred in flag leaf tip,whole flag leaf and top second leaf,respectively.After being infected by A.besseyi,all the experimental materials could sprout normally,but their plant height,panicle length,seed-setting rate and 1000-grain weight were affected to varying degrees.In addition,after being infected by A.besseyi,various symptomatic tissues of R161 exerted different effects on rice yield.Especially,panicles with withered and twisted whole flag leaf were most affected.[Conclusion]This study provided the basis for further exploration of the damages of A.besseyi infection to rice and development of corresponding control measures.展开更多
A study was done on the relationship between Aphelenchoides besseyi and the symptoms of small grains and erect panicles. This is an important rice disease in Jiangsu Province, China. A. besseyi was extracted from smal...A study was done on the relationship between Aphelenchoides besseyi and the symptoms of small grains and erect panicles. This is an important rice disease in Jiangsu Province, China. A. besseyi was extracted from small grains and erect panicles and cultured artificially, and then inoculated into bud and leaf sheaths of seedlings of two Oryza sativa cultivars, namely Zhendao 2 and Wuyunjing 7 in a greenhouse. The effect on rice growth, in particular the small grains and erect panicles, was revealed by the extent of the disease, seed expansion stages, nematode load, and nematode mortality. In contrast to healthy seedlings, the height, length, and the numbers of spikelets of unhealthy panicles of Zhendao 2 were decreased by 6.7, 16.4, and 13.5%, respectively. Before anthesis, nematodes were attracted to the leaf sheath and apical meristem, nematode load increased by 40%; after anthesis, nematodes occurred in spikelets principally and the number increased by 90.8%. The percentages of infected seeds and nematode load were highest in plump seeds and lowest in empty seeds. Nematode mortality on grain with normal endosperm was lower than seeds with abnormal endosperm. Results indicated that A. besseyi was the pathogen in rice with the symptoms of small grains and erect panicles. Wuyunjing 7 manifests only the small grains and erect panicles symptoms and not the symptoms of leaf white-tip. These symptoms of small grains and erect panicles are new symptom records for the disease caused by A. besseyi on rice.展开更多
The leafing rates of fourteen rice varieties were measured in a sowing-time experiment,and a rapid leafing genotype at the vegetative growth stage was discovered in an indica variety Yanhui 559.The leaf number on the ...The leafing rates of fourteen rice varieties were measured in a sowing-time experiment,and a rapid leafing genotype at the vegetative growth stage was discovered in an indica variety Yanhui 559.The leaf number on the main culm of Yanhui 559 was always 4-5 leaves more than that of Lemont,and the leafing rate of Yanhui 559 was significantly higher than that of Lemont based on similar growth durations from sowing to heading.Furthermore,the difference of the leafing rate was significant at the vegetative growth stage,but not distinctive at the panicle initiation stage.Genetic analysis of the leafing rates in the two backcross populations of Yanhui 559 and Lemont showed that major and quantitative genes controlled the expression of rapid leafing character.Based on results of investigation for some plants with similar growth durations in the backcrossing populations,the rapid leafing genotypes exhibited earlier tillering and more tiller numbers per plant,and its yield components including the number of panicles per plant and number of grains per panicle were superior to those of the slow leafing genotypes.Further research and application feasibility of the rapid leafing genotype in breeding were discussed.展开更多
摘要Field experiments were carried out with rice variety of Shennong 91 of short culms and erect panicles to study nutrient contents in high-yielding rice plants and to increase rice yield by appropriate fertilization.Nitrogen, phosphorus, potassium, magnesium, zinc, manganese, iron and copper contents in rice plants varied with different treatment factors. The relationship between the nutrient contents and treatment factors could be simulated using a multiple quadratic equation. The nutrient contents in plants should be appropriate for high-yielding rice. If the mean nutrient content in rice plants producing 11 t ha-1 or more of grain (μj) was set as the standard value and the standard deviation (σj) was set as the range of variation, the nutrient content in high-yielding rice plants should be μj±1.99σj. Rice leaves were sensitive to the nutrient elements. Heavy nitrogen dressing increased the content of nitrogen in rice plants. Sparse transplanting also increased nitrogen content. Improper application of nitrogen, phosphorus and potassium could affect the nutrient contents and decrease the grain yield.
基金supported by the National Basic Research Program (973) of China (No.2010CB126200)China Postdoctoral Science Foundation Project (No.20090451437)
摘要Detection of crop health conditions plays an important role in making control strategies of crop disease and insect damage and gaining high-quality production at late growth stages. In this study, hyperspectral reflectance of rice panicles was measured at the visible and near-infrared regions. The panicles were divided into three groups according to health conditions: healthy panicles, empty panicles caused by Nilaparvata lugens St^l, and panicles infected with Ustilaginoidea virens. Low order derivative spectra, namely, the first and second orders, were obtained using different techniques. Principal component analysis (PCA) was performed to obtain the principal component spectra (PCS) of the foregoing derivative and raw spectra to reduce the reflectance spectral dimension. Support vector classification (SVC) was employed to discriminate the healthy, empty, and infected panicles, with the front three PCS as the in- dependent variables. The overall accuracy and kappa coefficient were used to assess the classification accuracy of SVC. The overall accuracies of SVC with PCS derived from the raw, first, and second reflectance spectra for the testing dataset were 96.55%, 99.14%, and 96.55%, and the kappa coefficients were 94.81%, 98.71%, and 94.82%, respectively. Our results demonstrated that it is feasible to use visible and near-infrared spectroscopy to discriminate health conditions of rice panicles.
摘要The abnormal rice with small grains and erect panicles were found on a large scale in China, which showed shortend rice panicle and decreased number of grains in comparison with normal rice, and the grain was small and black-brown, and some of them were distorted, while the flag leaf was normal. A kind of nematode of Aphelenchoides was isolated from the grains of rice variety Wuyujing 3 which performed 'small grains and erect panicles' symptom. There were 2014 nematodes in one hundred grains infected, and up to 74 in single grain, 92 percent of the grains tested had nematodes in the infested panicles. The diagnosis characters of nematode include lateral fields about one-fourth as wide as body, with 4 incisures. The terminus bears a mucro of diverse shape with 3-4 pointed processes. The female post-vulval uterine sac extends less than 50% of distance from vulva to anus, no sperm in it. Oocytes usually arrange in 2-4 rows. The male spicules have a moderately developed rostrum. Morphological measurements showed it to be conspecific with Aphelenchoides besseyi Christie, 1942.
基金supported by the National Natural Science Foundation of China (U21A20205)Key Projects of Natural Science Foundation of Hubei Province (2021CFA059)+1 种基金Fundamental Research Funds for the Central Universities (2021ZKPY006)cooperative funding between Huazhong Agricultural University and Shenzhen Institute of Agricultural Genomics (SZYJY2021005,SZYJY2021007)。
摘要Self-occlusions are common in rice canopy images and strongly influence the calculation accuracies of panicle traits. Such interference can be largely eliminated if panicles are phenotyped at the 3 D level.Research on 3 D panicle phenotyping has been limited. Given that existing 3 D modeling techniques do not focus on specified parts of a target object, an efficient method for panicle modeling of large numbers of rice plants is lacking. This paper presents an automatic and nondestructive method for 3 D panicle modeling. The proposed method integrates shoot rice reconstruction with shape from silhouette, 2 D panicle segmentation with a deep convolutional neural network, and 3 D panicle segmentation with ray tracing and supervoxel clustering. A multiview imaging system was built to acquire image sequences of rice canopies with an efficiency of approximately 4 min per rice plant. The execution time of panicle modeling per rice plant using 90 images was approximately 26 min. The outputs of the algorithm for a single rice plant are a shoot rice model, surface shoot rice model, panicle model, and surface panicle model, all represented by a list of spatial coordinates. The efficiency and performance were evaluated and compared with the classical structure-from-motion algorithm. The results demonstrated that the proposed method is well qualified to recover the 3 D shapes of rice panicles from multiview images and is readily adaptable to rice plants of diverse accessions and growth stages. The proposed algorithm is superior to the structure-from-motion method in terms of texture preservation and computational efficiency. The sample images and implementation of the algorithm are available online. This automatic, cost-efficient, and nondestructive method of 3 D panicle modeling may be applied to high-throughput 3 D phenotyping of large rice populations.
基金Supported by Science and Technology Project for Grain Production in National Science and Technology Support Program(2011BAD16B11-02YJ012012BAD04B01-02)+1 种基金Key Scientific and Technological Project of Heilongjiang Province(GA13B101)Fund for Distingnished Young Scholars of Heilongjiang Academy of Agricultural Sciences(2014)
摘要Under field conditions, three erect panicle type japonica dee cultivars and three curved panicle type japonica rice cultivars were applied as experimental materials to investigate the differences of protein composition of grains at different positions in panicles between two different panicle types of japonica rice. Accord- ing to the results, the panicle type of japonica rice had no direct correlation with albumin content, globulin content, prolamin content and glutelin content in rice, variations of protein composition of grains at different positions in panicles. There were certain correlations between soluble protein contents at different grain posi- tions in the same panicle and the flowering order of glumous flowers in the panicle. Albumin content, prolamin content and glutelin content in grains on secondary rachis branches of two panicle types (erect and curved) of japonica rice cuhivars were higher than that on primary rachis branches, while globulin content exhibited an opposite trend. Globulin content, prolamin content and glutelin content in grains at different positions demonstrated a descending order of bottom 〉 middle 〉 top, while albumin content exhibited an opposite trend. The interactions between primary and secondary rachis branches and among top, middle and bottom rachis branches significantly affected soluble protein contents.
摘要Panicles of an indica rice line TM7-5 were subjected to radiation with 137^Cs gamma rays at 0 (control), 5, 10, 15 and 20 Gy respectively, and then its anthers were cultured. There were slight differences among the treatments in peak emerging time of callus initiation, from 38 to 44 days after inoculation (DAI) as well as the frequency of callus initiation (2.3-3.5%). About two thirds calli were induced before 44 DAI, and calli derived beyond 60 DAI lost the regeneration ability. Green plant regeneration frequency was significantly stimulated from two- to three-fold by irradiation of the 1370S gamma rays compared with the control, and the maximum was 22,81% (15 Gy). The culture ability based on callus initiation and green plantlet regeneration was 0.19% for the control while it was over 0.45% for all the irradiated treatments, and the maximum was 0,59% for 15 Gy treatment. The advantages of panicle irradiation before anther culture and the potential application in rice anther culture, especially for recalcitrant indica rice, were discussed.
基金supported by the National Natural Science Foundation of China(32372214 and 32272198)the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX(23)1035)+2 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD-2020-01)the Top Talent Supporting Program of Yangzhou University,China(YZU-2028-01)the Hong Kong Research Grants Council,China(GRF 12101722,12102423,and 12105824)。
摘要Highlights Improving spikelet production efficiency(SPE)can further enhance the grain yield and harvest index in rice.Higher SPE can enhance post-anthesis photoassimilate production in the shoots of rice.Higher SPE can promote the post-anthesis remobilization of non-structural carbohydrate(NSC)from rice stems.Rice grain yield is a complex agronomic trait determined by four key components:panicles per plant,spikelets per panicle,filled-grain rate,and grain weight.
摘要Fungal endophytes are suggested to manipulate host grass reproduction.Using different grass-endophyte combinations in a four year common garden experiment,we show that endophyte can retain control over the reproductive functions of the host.Plants were collected as seeds from two different environments,meadows and riverbanks.Natural endophyte infection(E+)increased reproductive effort of plants;13 and 15%higher proportion of E+plants produced panicles compared to naturally uninfected(E-)and manipulatively endophyte-infected(ME+)plants,respectively.Meadoworigin E+plants produced also higher number of panicles compared to E-in the last two years,and both meadow-and riverbank-origin ME+plants produced higher number of panicles compared to ME-plants in most of the years.Pseudovivipary was recorded in 5%of the plants each year.Pollen limitation appears not to induce pseudovivipary.Both E+and ME+plants produced higher number of seminiferous panicles compared to E-and ME-plants.The higher proportion of pseudoviviparism in ME+plants compared to E+,Eand ME-plants from meadows suggests specific genotypegenotype cross-talk between endophyte and grass.Pseudovivipary was affected by year and primarily explained by plant reproductive effort since most vigorous plants produced more pseudoviviparous panicles.We propose that endophytes can promote host grass reproduction in a habitatspecific manner and depending on genetic compatibility between partners.However,reproductive behavior depended on maternal habitat and the annual environmental conditions highlighting the importance of long-term experiments to estimate the effects of endophytes on host plant ecology.
基金supported by the Natural Science Foundation of Jiangsu Province(BK20221279)the Zhongshan Biological Breeding Laboratory(ZSBBL-KY2023-01-01)+5 种基金the Core Provenance Project in Jiangsu Province(JBGS[2021]005)the National Natural Science Foundation of China(32372141)the Key Research and Development Program of Jiangsu Province Modern Agriculture(BE2023362)the Key Research and Development Program of Yangzhou Modern Agriculture(YZ2024053)the Earmarked Fund for the China Agricultural Research System(CARS-01-78)the Special Fund for Scientific Research Development of the Institute of Agricultural Sciences for Lixiahe Region in Jiangsu(SJ(22)103 and SJ(22)109)。
摘要Rice blast is one of the most devastating diseases,particularly panicle blast leads to significant yield losses.Utilization of panicle blast resistance genes is one of the effective ways to control this disease.However,genetic resources with panicle blast resistance are rare.The objective of this study was to identify the role of OsEXOⅢ-1,a previously identified candidate gene,in Pigm-mediated panicle blast resistance in rice.There were two main haplotypes in rice germplasm,with OsEXOⅢ-1~(Hap1),the product of the resistant haplotype,binding more strongly to PiBP1 than OsEXOⅢ-1~(Hap2).OsEXOⅢ-1/PiBP1 positively regulates Pigm-mediated panicle blast resistance by regulating expression of PR1 subfamily genes.OsEXOⅢcould be a target for breeding panicle blast-resistant cultivars.
基金supported by the grants from the National Natural Science Foundation of China(32372490 and 31772241 to J.F.,32472575 to W.M.W.,and 32202263 to G.B.L.).
摘要Rice false smut,caused by the fungal pathogen Ustilaginoidea virens,is a notorious panicle disease.It significantly compromises grain yield and quality and results in the contamination of rice with mycotoxins,thereby posing significant risks to both human and animal health(Yu et al.,2023).Given the current limited availability of genetic resistance resources to combat this pathogen,identifying susceptibility factors in rice is a critical prerequisite for developing durable and effective disease management strategies(Huang et al.,2024).
基金supported by the Biological Breeding-National Science and Technology Major Project,China(Grant No.2023ZD0406801)the National Natural Science Foundation of China(Grant No.32300278)+2 种基金the Key R&D Plan of Shandong Province,China(Grant No.2024LZGC009)the Innovation Program of Chinese Academy of Agricultural Sciences(Grant No.CAAS-CSCB-202402)the Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences,China(Grant No.CXGC2025B09).
摘要Panicle number per plant,grain number per panicle,and grain weight are three key factors influencing rice grain yield.Gn1a,a major QTL for grain number per panicle,encodes the cytokinin oxidase/dehydrogenase(CKX)OsCKX2.While the use of elite Gn1a alleles has been well documented in indica rice cultivars,their potential in japonica rice remains largely unexplored.In this study,we characterized three suppressor mutants of the rice cytokinin receptor mutant pal1/ohk4 and found that all causal genes were novel alleles of Gn1a identified through the MutMap approach.These three suppressor mutants caused single amino acid substitutions in the FAD-binding domain(G556D and G156D)and the cytokinin-binding domain(Y357C),resulting in significantly reduced enzymatic activity of OsCKX2 and elevated cytokinin levels in the panicle.Haplotype analysis of Gn1a using a natural population from the 3K Rice Genomes Project showed that G556D,G156D,and Y357C were novel alleles of Gn1a.G556,G156,and Y357 were highly conserved,whereas four natural variants G54A,A105V,H116R,and N535K identified in different haplotypes of Gn1a showed extremely low conservation.By backcrossing the suppressor mutants with their original wild-type Huaidao 5,an elite japonica rice variety,we developed improved lines carrying only the gn1a mutation.The improved lines showed a significant increase in grain number per panicle,grain weight,panicle number per plant,plant height,and stem thickness,leading to a 25.7%-28.7%increase in grain yield per plot compared with Huaidao 5.This study provides valuable Gn1a alleles for synergistic improvement of the three key yield factors and offers germplasm resources for high-yielding breeding in japonica rice.
摘要High-throughput phenotyping is the bottleneck for advancing field trait characterization and yield improvement in major field crops.Specifically for sorghum(Sorghum bicolor L.),rapid plant-level yield estimation is highly dependent on characterizing the number of grains within a panicle.In this context,the integration of computer vision and artificial intelligence algorithms with traditional field phenotyping can be a critical solution to reduce labor costs and time.Therefore,this study aims to improve sorghum panicle detection and grain number estimation from smartphone-capture images under field conditions.A preharvest benchmark dataset was collected at field scale(2023 season,Kansas,USA),with 648 images of sorghum panicles retrieved via smartphone device,and grain number counted.Each sorghum panicle image was manually labeled,and the images were augmented.Two models were trained using the Detectron2 and Yolov8 frameworks for detection and segmentation,with an average precision of 75%and 89%,respectively.For the grain number,3 models were trained:MCNN(multiscale convolutional neural network),TCNN-Seed(two-column CNN-Seed),and Sorghum-Net(developed in this study).The Sorghum-Net model showed a mean absolute percentage error of 17%,surpassing the other models.Lastly,a simple equation was presented to relate the count from the model(using images from only one side of the panicle)to the field-derived observed number of grains per sorghum panicle.The resulting framework obtained an estimation of grain number with a 17%error.The proposed framework lays the foundation for the development of a more robust application to estimate sorghum yield using images from a smartphone at the plant level.
基金funded by grants from the National Natural Science Foundation of China (31930006 to Y.W.)the National Key Research and Development Program of China (2022YFF1002903 to Y.W.)+1 种基金the Top Talents Program “One Case One Discussion”(Yishiyiyi to Y.W.)from Shandong provinceShandong Agricultural University Talent Introduction Start-up Fund (to N.Z.)
摘要Branching is a critical aspect of plant architecture that significantly impacts the yield and adaptability of staple cereal crops like rice and wheat.Cereal crops develop tillers during the vegetative stage and panicle or spike branches during the reproductive stage,respectively,both of which are significantly impacted by hormones and genetic factors.Tillering and panicle branching are closely interconnected and exhibit high environmental plasticity.Here,we summarize the recent progress in genetic,hormonal,and environmental factors regulation in the branching of rice and wheat.This review not only provides a comprehensive overview of the current knowledge on branching mechanisms in rice and wheat,but also explores the prospects for future research aimed at optimizing crop architecture for enhanced productivity.
基金Supported by the Green Plant Protection Project in Heilongjiang Province(2130108)the Key R&D Program Project of Heilongjiang Province(2023ZX02B0502)the Heilongjiang Province Rice Modern Agriculture Industry Technology Collaborative Innovation System Project(2025)。
摘要This study aimed to clarify the pathogen composition,biological characteristics,infection patterns,and effective control agents for panicle blight of rice in Heilongjiang Province.Diseased panicles were collected from different rice-growing areas in Heilongjiang Province and subjected to tissue isolation,pathogenicity tests,morphological observation,and molecular identification.The primary pathogens identified were Fusarium graminearum,Alternaria alternata and Nigrospora oryzae.The biological characteristics of these three pathogens were systematically investigated.Pathogenicity assays revealed that F.graminearum exhibited the strongest pathogenicity,followed by A.alternata,while N.oryzae was the weakest.In vitro toxicity tests screened highly effective fungicides:75%trifloxystrobin-tebuconazole showed the best inhibitory effect against F.graminearum(EC50=0.0140μg·mL-1);30%tebuconazole-azoxystrobin was the most effective against A.alternata(EC50=0.0060μg·mL-1)and N.oryzae(EC50=0.0310μg·mL-1).
基金supported by the Hunan Provincial Natural Science Foundation of China(Grant No.2023JJ40309)the Changsha Outstanding Innovative Youth Training Program(kq2306015).
摘要Grain filling is a critical determinant of yield and quality in rice.This study aims to clarify the association between grain photosynthesis and the filling rate of rice varieties with different grain weights,providing a theoretical foundation for optimizing grain-filling processes.Two rice varieties with similar growth duration but different grain weights were selected:a large-grain variety,Lingliangyou 268(L268),and a small-grain variety,Ruiliangyou 1053(R1053).Differences in grain filling,grain photosynthetic rate,and grain chlorophyll content were systematically examined during the filling stage.Results showed significant differences in grain-filling,grain photosynthetic rate,and grain chlorophyll content between large-grain and small-grain rice varieties.The grain photosynthetic rate of L268 was a significantly higher than R1053.L268 also exhibited significantly higher initial grain filling rate,maximum grainfilling rate,and mean grain filling rate compared to R1053.Throughout the grain filling period,L268 showed higher grain chlorophyll content(including chlorophyll a,chlorophyll b,and total chlorophyll)than R1053.The increase in chlorophyll content,particularly total chlorophyll,enhanced the grain photosynthetic rate during the early and middle stages of grain filling significantly.These findings suggested that rice varieties with higher grain weights exhibited stronger panicle photosynthetic capacity due to their higher chlorophyll content.The enhanced grain photosynthetic rate contributed to improved grain filling and increased grain weight.
基金supported by the National Natural Science Foundation of China(Grant Nos.32000377,32172037,and 32472211)the Biological Breeding-National Science and Technology Major Project,China(Grant No.2023ZD04068)+2 种基金the Fundamental Research Funds for the Central Universities,China(Grant No.KJQN202103)the open funds of the State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,China(Grant No.ZW202401)the Cyrus Tang Innovation Center for Crop Seed Industry,China.
摘要Rice grain yield is primarily determined by three key agronomic traits:panicle number,grain number per panicle,and grain weight(GW).However,the inherent tradeoffs among these yield components remain a persistent challenge in rice breeding programs.Notably,compared with GW,brown rice weight(BRW)provides a more direct metric associated with actual grain yield potential.In this study,we conducted a two-year replicated genome-wide association study to elucidate the genetic architecture of BRW and identify new loci regulating GW.Among seven consistently detected loci across experimental replicates,four were not co-localized with previously reported genes associated with BRW or GW traits.BRW1.1,one of the four newly identified loci,was found to encode a novel RNA-binding protein.Functional characterization revealed that BRW1.1 acts as a negative regulator of BRW,potentially through modulating mRNA translation processes.Intriguingly,through integrated analysis of mutant phenotypes and haplotype variations,we demonstrated that BRW1.1 mediates the physiological tradeoff between GW and panicle number.This study not only delineates the genetic determinants of BRW but also identifies BRW1.1 as a promising molecular target for breaking the yield component tradeoff in precision rice breeding.
基金the National Natural Science Foundation of China (Nos.11672183,91641129 and 91441205)supported by Airbreathing Hypersonic Laboratory (AHL) of China Aerodynamics Research and Development Center (CARDC)
摘要In the last several decades, Particle Image Velocimetry(PIV) has reached a high degree of maturity as a laser diagnostic technique based on tracer particles, with significant improvements in accuracy, resolution, dynamic range, and as an extension to combustion measurements. To assess the recent developments and to project the future trends of using the PIV technique for combustion measurements, we review many key issues for measuring combustion flow fields. We introduce the representative applications of a supersonic combustor and swirling burner and summarize the promising prospects and further development requirements of PIV measurements in combustion flow fields.
摘要[Objective]This study aimed to reveal the effects of Aphelenchoides besseyi infection on different rice varieties(lines).[Method]By field observation and indoor phenotypic investigation,four conventional japonica rice varieties(lines)and japonica rice restorer line R161 under natural onset conditions were observed and analyzed.[Result]After being infected by A.besseyi,different rice varieties(lines)exhibited various symptoms.Specifically,Ning 1707,Ning 1818,Zhendao 88 and Nanjing 9108 had withered leaf tips and exhibited the symptoms of"small grains and erect panicles";japonica rice restorer line R161 only had withered leaf tips without symptoms of"small grains and erect panicles",and the withering symptoms occurred in flag leaf tip,whole flag leaf and top second leaf,respectively.After being infected by A.besseyi,all the experimental materials could sprout normally,but their plant height,panicle length,seed-setting rate and 1000-grain weight were affected to varying degrees.In addition,after being infected by A.besseyi,various symptomatic tissues of R161 exerted different effects on rice yield.Especially,panicles with withered and twisted whole flag leaf were most affected.[Conclusion]This study provided the basis for further exploration of the damages of A.besseyi infection to rice and development of corresponding control measures.
基金the National High Technology Research and Development Program(863) of China(2001AA249021)
摘要A study was done on the relationship between Aphelenchoides besseyi and the symptoms of small grains and erect panicles. This is an important rice disease in Jiangsu Province, China. A. besseyi was extracted from small grains and erect panicles and cultured artificially, and then inoculated into bud and leaf sheaths of seedlings of two Oryza sativa cultivars, namely Zhendao 2 and Wuyunjing 7 in a greenhouse. The effect on rice growth, in particular the small grains and erect panicles, was revealed by the extent of the disease, seed expansion stages, nematode load, and nematode mortality. In contrast to healthy seedlings, the height, length, and the numbers of spikelets of unhealthy panicles of Zhendao 2 were decreased by 6.7, 16.4, and 13.5%, respectively. Before anthesis, nematodes were attracted to the leaf sheath and apical meristem, nematode load increased by 40%; after anthesis, nematodes occurred in spikelets principally and the number increased by 90.8%. The percentages of infected seeds and nematode load were highest in plump seeds and lowest in empty seeds. Nematode mortality on grain with normal endosperm was lower than seeds with abnormal endosperm. Results indicated that A. besseyi was the pathogen in rice with the symptoms of small grains and erect panicles. Wuyunjing 7 manifests only the small grains and erect panicles symptoms and not the symptoms of leaf white-tip. These symptoms of small grains and erect panicles are new symptom records for the disease caused by A. besseyi on rice.
基金This work was supported by the Science and Technology Project Cooperating among Two Provinces and One City in the Yangtze River Delta(Grant No.BE2004399).
摘要The leafing rates of fourteen rice varieties were measured in a sowing-time experiment,and a rapid leafing genotype at the vegetative growth stage was discovered in an indica variety Yanhui 559.The leaf number on the main culm of Yanhui 559 was always 4-5 leaves more than that of Lemont,and the leafing rate of Yanhui 559 was significantly higher than that of Lemont based on similar growth durations from sowing to heading.Furthermore,the difference of the leafing rate was significant at the vegetative growth stage,but not distinctive at the panicle initiation stage.Genetic analysis of the leafing rates in the two backcross populations of Yanhui 559 and Lemont showed that major and quantitative genes controlled the expression of rapid leafing character.Based on results of investigation for some plants with similar growth durations in the backcrossing populations,the rapid leafing genotypes exhibited earlier tillering and more tiller numbers per plant,and its yield components including the number of panicles per plant and number of grains per panicle were superior to those of the slow leafing genotypes.Further research and application feasibility of the rapid leafing genotype in breeding were discussed.