Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A random...Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A randomized control trial parallel group design was conducted among 45 comatose patients divided into two intervention groups,i.e.nurse voice stimulus group,receiving nurses voice with standard care,family members voice stimulus group receiving their beloved family member voice with standard care and one control group receiving only standard care in medicine intensive care unit.The intervention was provided three times a day,each lasting 5 min for 7 days in addition to standard care.Repeated measure analysis of variance and independent t-test were used to compare within and between groups,respectively.Results:The study found significant differences in Glasgow coma scale(GCS)scores within both the nurse(F=2.78,P=0.042)and family member(F=10.27,P=0.0001)voice stimulus groups over 7 days.Comparing GCS scores between intervention groups showed significant variations before(P=0.028),during(P=0.047),and after(P=0.036)the intervention on day 7.Comparing GCS scores between the family members’voice stimulus group and the control group,significant changes were observed on days 5 and 7(P=0.043,0.030,0.030,and 0.014,0.012,0.012)before,during,and after the intervention.Conclusions:The use of beloved family members’voices proved more effective in elevating the patients’level of consciousness compared to both the nurse voice stimulus group and the control group.展开更多
In May of 2007,the second generation selected (SS) and control (SC) groups of pearl oyster Pinctada martensii were established by selecting 10% breeders with the largest and mean shell length,respectively,from the...In May of 2007,the second generation selected (SS) and control (SC) groups of pearl oyster Pinctada martensii were established by selecting 10% breeders with the largest and mean shell length,respectively,from the first generation selected group.Growth performance of the SS and SC groups were compared on the basis of measurement data at Days 8,18,60,95,195 and 365.On Day 365,100 individuals (60.0–75.0 mm at shell length) were sampled from each group and then subjected to the experiment where physiological parameters such as filtrate rate,oxygen consumption and ammonia excretion were measured at 15,20,25 and 30°C.The results show that the SS group had significantly larger mean shell length and shell height than the SC group at Days 8,18,60,95,195 and 365 (P 0.05).The genetic gains at different ages varied from 6.0% to 17.0% for shell length and 5.7% to 14.6% for shell height,respectively.At 15,20,25 and 30 ° C,the SS groups had significantly larger filtrate rate than the SC group (P 0.05).At 15 and 25 °C,the differences in oxygen consumption rate between the SS and SC groups were not significant (P 0.05).At 20 and 30 °C,however,the oxygen consumption rate of the SS group was significantly larger than the SC group (P 0.05).At 15,20,25 and 30 °C,there were no significant differences in ammonia excretion rate between the SS and SC groups (P 0.05).The present results indicate that there existed considerable genetic variability in the base population and a further selection could be likely fruitful.Mass selection for faster growth might produce animals that had higher intake of metabolizable energy by virtue of faster filtrating behavior.展开更多
The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on co...The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings (chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81) under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.展开更多
[ Objective] This study aimed to investigate the effects of 1-naphthol on primary productivity in aquatic ecosystems. [ Method] The effects of 1-naphthol on biomass, chlorophyll a content, malondialdehyde (MDA) cont...[ Objective] This study aimed to investigate the effects of 1-naphthol on primary productivity in aquatic ecosystems. [ Method] The effects of 1-naphthol on biomass, chlorophyll a content, malondialdehyde (MDA) content and soluble protein content of Chlorella vulgaris were investigated. [ Result] 1-Naphthol could significantly inhibit the growth of Chlorella vulgaris. On the 7^th d, the biomass of Chlorella vulgaris exposed to 1.0, 3.0, 6.0, 12.0 and 18.0 mg/L 1-naphthol was 60.26%, 48.15%, 41.32%, 28.59% and 27.10% of that in control respectively, exhibiting a significant dose-response relationship. Chlorella vulgaris was exposed to different concentrations of 1-naphthol (0, 1,5 and 10 mg/L) for 7 d ; with the increase of 1-naphthol concentration, chlorophyll a content of Ch/orella vulgaris was reduced, MDA content increased gradually, soluble protein content increased first and then declined. [ Conclusion] According to the experimental results, 1-naphthol can significantly inhibit the growth of Chlorella vulgaris, thus reducing primary productivity in aquatic ecosystems.展开更多
Exposure to sound,heat,and increased physical workload can change physiological parameters.This study was conducted to evaluate the effect of concomitant exposure to sound,heat,and physical workload changes on physiol...Exposure to sound,heat,and increased physical workload can change physiological parameters.This study was conducted to evaluate the effect of concomitant exposure to sound,heat,and physical workload changes on physiological parameters in controlled laboratory conditions.This experimental crosssectional study was conducted in 35 male university students with a mean age of 25.75 years and a mean BMI of 22.69 kg/m2.Systolic and diastolic blood pressure and heart rate were measured after 15 min rest in the laboratory,5 and 10 min after starting the experiment,and then after 20 min in controlled laboratory conditions in five combination modes.The combination modes were(Sound:65 dB,WBGT:22°C,Speed:1.7,Slope:10%),(Sound:65 dB,WBGT:22°C,Speed:3.4,Slope:14%),(Sound:95 dB,WBGT:22°C,Speed:1.7,Slope:10%),(Sound:65 dB,WBGT:32°C,Speed:1.7,Slope:10%),and(Sound:95 dB,WBGT:32°C,Speed:3.4,Slope:14%).Mixed model analysis and paired t-test were applied for analysis.The results showed that the mean physiological parameters(Systolic and diastolic blood pressure and heart rate)increased when different combination modes worsened(Sound from 65 to 95 dB,WBGT from 22°C to 32°C,speed from 1.7 to 3.4,and slope from 10%to 14%,and when sound:95 dB,WBGT:32°C,Speed:3.4,and Slope:14%).Moreover,the mean changes of systolic and diastolic blood pressure were significant in all conditions when compared with the reference condition(Sound:65 dB,WBGT:22°C,Speed:1.7,and Slope:10%).The mean heart rate changes were also significant except for exposure to the second condition(Sound:65 dB,WBGT:22°C,Speed:3.4,Slope:14%)and the third condition(Sound:95 dB,WBGT:22°C,Speed:1.7,Slope:10%).Exposure to hazardous levels of sound,heat,and workload has adverse effects on physiological parameters.Concomitant exposure to all three hazards has a synergistic effect and increases the adverse effect.展开更多
The study was conducted to investigate the characteristics of blood physiological parameters of Apodemus agrarius (A. agrarius). Blood physiological pa- rammers of 10 A. agrarius (half male and half female) were d...The study was conducted to investigate the characteristics of blood physiological parameters of Apodemus agrarius (A. agrarius). Blood physiological pa- rammers of 10 A. agrarius (half male and half female) were determined by automated hematology analyzer. Results showed that difference of A. agrarius hematokrit (HCT) between male and female was significant (P 〈 0.05 ), and differences of all the other blood physiological parameters between male and female were not sig- nificant ( P 〉 0.05 ). In the study, blood physiological reference value of A. agrarius was set up, which provided a basis for the bio-research and prophylaxis and treatment of A. agrarius.展开更多
[Objectives]This study was conducted to investigate the characteristics of physiological,hematological and blood biochemical parameters in introduced Hu sheep reared in the Zizhong area of Sichuan Province in spring.[...[Objectives]This study was conducted to investigate the characteristics of physiological,hematological and blood biochemical parameters in introduced Hu sheep reared in the Zizhong area of Sichuan Province in spring.[Methods]Ten healthy adult rams and ten healthy adult ewes aged 3-5 years were selected,and relevant parameters were measured.[Results]No significant differences were observed between rams and ewes in body temperature,respiratory rate,and heart rate(P>0.05).Among hematological parameters,except for a significantly higher total platelet count in ewes(P0.05).Among blood biochemical parameters,blood urea nitrogen(BUN),alanine aminotransferase(ALT),and the urea nitrogen-to-creatinine ratio were significantly higher in rams than in ewes(P0.05)were observed between rams and ewes in 13 indicators,including total protein,albumin,glucose,and creatine kinase(CK).Overall,the physiological,hematological,and blood biochemical parameters of Hu sheep under housed conditions in the Zizhong area remained stable,with only a few indicators showing significant differences between sexes.This pattern reflects subtle physiological regulatory differences in metabolism and immune function between rams and ewes.[Conclusions]This study provides fundamental data for understanding the physiological adaptability of Hu sheep in the region.展开更多
[Objectives]This study was conducted to evaluate the physiological adaptability of housed Hu sheep introduced to the Zizhong area of Sichuan Province under high-temperature summer conditions.[Methods]Adult male and fe...[Objectives]This study was conducted to evaluate the physiological adaptability of housed Hu sheep introduced to the Zizhong area of Sichuan Province under high-temperature summer conditions.[Methods]Adult male and female Hu sheep(10 per group)reared in a housing system in this region were assessed by measuring physiological,hematological and blood biochemical parameters.[Results]The body temperature of rams was significantly higher than that of ewes(P0.05).Ewes exhibited significantly higher values than rams in several blood physiological parameters,including total leukocyte count,lymphocyte count,hemoglobin content,hematocrit,and mean corpuscular hemoglobin content(P<0.05).No significant differences were detected in the remaining six blood physiological parameters between rams and ewes.Regarding blood biochemical indicators,ewes showed significantly elevated levels of albumin,cholesterol,alkaline phosphatase,and the blood urea nitrogen/creatinine ratio(P<0.05),while rams had significantly higher creatinine content(P<0.05).No significant differences were found between sexes in other 11 blood biochemical indicators,including total protein,globulin,and glucose.These findings suggest that under summer conditions in the Zizhong area,housed Hu sheep exhibit sex-based differences in physiological adaptability,with ewes displaying enhanced blood oxygen-carrying capacity and immune-related indicators compared to rams.[Conclusions]The study provides valuable insights for understanding the adaptation mechanisms of Hu sheep and developing gender-specific management strategies during subtropical summers.展开更多
[Objective] The aim was to evaluate the health of transgenic sFat-1 gene pigs in order to provide a reference for the breeding selection of pig excellent individuals. [Method] 15 physiological biochemical parameters a...[Objective] The aim was to evaluate the health of transgenic sFat-1 gene pigs in order to provide a reference for the breeding selection of pig excellent individuals. [Method] 15 physiological biochemical parameters and reproductive performances of 21 negative control pigs and 20 transgenic sFat-1 gene pigs were determined. [Result] The liver functions of some transgenic sFat-1 gene pigs were hurted lightly,and the reproductive performance also decreased lightly. [Conclusion] The determination of physiological biochemical parameters and reproductive performance in transgenic pigs could be taken as one of effective methods in environmental safety evaluation of transgenic pigs.展开更多
Split-root solution culture was used to study the promoting effect of lanthanum on rice (Oryza sativa) growth and its physiological mechanisms. Results sho w that low concentration (0.05~1.5 mg·L -1) increases r...Split-root solution culture was used to study the promoting effect of lanthanum on rice (Oryza sativa) growth and its physiological mechanisms. Results sho w that low concentration (0.05~1.5 mg·L -1) increases rice yield an d grain numbers. High concentration depresses grain formation (9~30 mg·L -1 ) and root elongation (1.5~30 mg·L -1). No significant influence on str aw dry weight was found over the whole concentration range except the 0.05 mg·L -1 treatment. With the increase of La concentration from 0.05 to 0.75 mg· L -1, catalase (CAT) activity in the first fully expandeing leaves and root s decreases. When La concentration is greater than 0.75 mg·L -1 or less than 9 mg·L -1, it significantly decreases superoxide dismutase activity ( SOD) in the leaves and roots. No significant effects were found on chlorophyll, protein and malondialdehyde (MDA) content. Possible mechanisms of La′s promotin g effect on rice growth and reduction effect of ·O- 2 were discussed.展开更多
Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed(Brassica napus L.)varieties.The present study aimed to assess the physiological and ana...Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed(Brassica napus L.)varieties.The present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes,P287(drought-tolerant)and T88(drought-sensitive)under three intensities of drought stress.All physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress conditions.At the fourth-leaf stage,the relative water content,chlorophyll content,protein content,malondialdehyde content,and the activities of peroxidase and catalase in P287 were significantly higher than those in T88,particularly under severe drought conditions.After rehydration,all physiological indexes recovered rapidly,especially in P287.In addition,under drought stress,compared with T88,P287 had thicker palisade tissue,thinner spongy tissue,higher ratio of chloroplast length to chloroplast width,higher stomatal density and stomatal closure rate.Overall,the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions.展开更多
In Algeria,the factors which contribute to the extension of the phenomenon of land salinization are linked to aridity and the climate which affects>95%of the territory,and the poor quality of irrigation water,to th...In Algeria,the factors which contribute to the extension of the phenomenon of land salinization are linked to aridity and the climate which affects>95%of the territory,and the poor quality of irrigation water,to the system of drainage often non-existent.or non-functional,our work in this context and shed light on the effects of salt stress on a sensitive legume.Our study aims to evaluate the effect of salt stress on physiological parameters,and the mycorrhizal activity of Phaseolus vulgaris L,Coco Rose variety and soil analyzes were also carried out.The latter are grown in pots subjected to salt stress with increasing concentrations of Nacl solution of 6%,9%and 12%.Our results confirm that saline stress induces an effect on the parameters studied to varying degrees compared to the control.However,a low content of Chlorophyll A,protein and sugars was recorded and an increase in the proline content as well as a negative influence on the physicochemical parameters of the soil each time the concentration increased.However,on mycorrhizal activity,the results showed a decrease in the colonization rate each time the“Nacl”concentration increased between 80,60 and 30%respectively for decreasing Nacl concentrations.展开更多
The objective of this work was to determine the effect of transport time (up to 11 hours) on animal welfare. 540 animals (cows, bulls and calves), three transport times of 2, 4 to 6, and 10 to 11 hours, and two sp...The objective of this work was to determine the effect of transport time (up to 11 hours) on animal welfare. 540 animals (cows, bulls and calves), three transport times of 2, 4 to 6, and 10 to 11 hours, and two space allowances (2 m2 and 1.5 m2 per animal for cows and bulls; and 1.2 m2 and 0.8 m2 per animal for calves) were used for the experiment during transport from farms to the abattoir in Uppsala, Sweden. Measurements were made on five animals on each trip. Stress response parameters considered were cortisol, glucose, lactate, creatine kinase and heart rate. Blood samples were taken before and after transport. Heart rate sensors were mounted on the animals at least 30 minutes before loading and measurements were made continuously from farms to the abattoirs until stunning. The results of heart rate measurement indicated that loading, un-loading and forced movement in the stunning box were the most stressful events. However, the results of statistical analysis confirmed that transport time doesn't have significant effect (P 〈 0.05) on heart rate. Concentration level of cortisol increased by 10 folds during short transport. However, cortisol concentration decreased with an increase of transport times (P 〈 0.01). Glucose concentrations increased with transport time in all animal categories (P ≤ 0.01). Transport time has significant effect on concentration levels of glucose (P ≤ 0.01), creatine kinase (P 〈 0.001) and lactate (P 〈 0.01) particularly after 6 hours journey time.展开更多
Exploring the response differences of leaf physiology parameters to enhanced nitrogen deposition between saplings and trees is vital for predicting the variations of terrestrial ecosystem structure and function under ...Exploring the response differences of leaf physiology parameters to enhanced nitrogen deposition between saplings and trees is vital for predicting the variations of terrestrial ecosystem structure and function under future global climate change. In this study, the ecophysiological parameters of saplings and trees of Fraxinus mandshurica Rupr. were measured at different levels of nitrogen addition in a temperate forest. The results show that ecophysiological parameters maximum net photosynthetic rate(Pmax), apparent quantum efficiency(a), dark respiration(Rd), light saturation point(Lsp), photosynthetic nitrogen use efficiency(PNUE),specific leaf area(SLA)and stomatal conductance under saturated light intensity(Gsmax) were higher in saplings than in trees. These physiological parameters and not Nleaf(leaf nitrogen content)led to relatively lower Pmaxand Rdin trees. For both saplings and trees, low and median nitrogen addition(23 and 46 kg ha-1a-1) resulted in significant increases in Pmax, Rd, Lsp, Chl, PNUE, SLA and Gsmax. These parameters tended to decline under high additions of nitrogen(69 kg ha-1a-1),whereas Nleaf was always enhanced with increasing nitrogen. Variations in Pmax and Rd with increasing nitrogen were attributed to variations in the strongly related parameters of, Lsp, Chl, PNUE, SLA and Gsmax. Overall, the response sensitivity of physiological parameters to enhanced nitrogen levels was lower in trees compared with saplings.展开更多
This study used non-invasive evaluation methods measured six skin physiological parameters of the lower lip in 180 subjects,including moisture content,transepidermal water loss(TEWL),smoothness(SESM),scaliness of the ...This study used non-invasive evaluation methods measured six skin physiological parameters of the lower lip in 180 subjects,including moisture content,transepidermal water loss(TEWL),smoothness(SESM),scaliness of the skin(SESC),wrinkles(SEW),and red area of the lip skin,and compared the effects of 6 groups of lip balms(no-additive group,marine oligosaccharides group,ceramides group,glycyrrhizinic acid group,allantoin group,and mixed group;30 each)on the skin physiological parameters of dry,flaking,and cracked lip subjects.The results showed that the lip mositure content of the subjects in the marine oligosaccharide group,glycyrrhetinic acid group,and allantoin group increased significantly by 44.40%,42.84%and 58.08%after 7 days of lip balm(P<0.05).The TEWL in the ceramide group and the allantoin group was significantly reduced by 21.83%and 24.72%,respectively,after 7 days of lip paste use(P<0.05).The lip skin smoothness values of subjects in the glycyrrhizic acid group and the allantoin group were significantly reduced by 18.76%and 14.97%,respectively,after 28 days of lip balm application(P<0.05).The lip skin scaling indices of subjects in the marine oligosaccharide group,the ceramides group,and the allantoin group were significantly reduced by 33.77%,42.69%,and 38.07%,respectively,after 28 days of lip balm application(P<0.05).The wrinkle parameters of the lip skin of the subjects in the marine oligosaccharide,glycyrrhizinic acid and allantoin groups were significantly reduced by 23.06%,23.29%and 25.98%,respectively,after 28 days of lip balm application(P<0.05).And the area of the red zone of the lip skin of the subjects in the allantoin group was significantly reduced by 4.27%,after 28 days of lip balm application(P<0.05).Combining the effects of the four active ingredients on the secretion of hyaluronic acid(moisturizing effect)and inflammatory factor(IL-6)in HSF cells,it suggests that marine oligosaccharides and allantoin have a perfect impact on enhancing the water content of the skin on the lips of the subjects,and further improve the symptoms of flaking and wrinkles on the lips of the subjects.The ceramide and allantoin can repair the skin barrier well and have a good effect on the chapped and flaky lips of the subjects.After 28 days of using lip balm,the water content of lips in the mixed group increased,the skin barrier was repaired and became smoother,and the wrinkles,scale index,and red zone value were reduced,which could well relieve chronic lip inflammation and lay a foundation for developing lip products for the treatment of chronic cheilitis.展开更多
Among all animals,dogs were domesticated the earliest and formed a unique bond with humans.Hare and Tomasello proposed that in ancestral canids,selection for individuals with reduced fear and aggression allowed them t...Among all animals,dogs were domesticated the earliest and formed a unique bond with humans.Hare and Tomasello proposed that in ancestral canids,selection for individuals with reduced fear and aggression allowed them to coexist in a niche adjacent to the human niche(Hare and Tomasello 2005).As a byproduct of this process,dogs acquired social cognitive abilities that facilitated communication with humans.展开更多
Many individuals face health risks owing to altered thermophysiological responses in non-uniform indoor thermal environments(e.g.,bathing),with cardiovascular disorders being a significant concern,particularly among o...Many individuals face health risks owing to altered thermophysiological responses in non-uniform indoor thermal environments(e.g.,bathing),with cardiovascular disorders being a significant concern,particularly among older adults.Experimental studies alone are insufficient to fully comprehend individual physiological responses and develop preventive strategies due to ethical and practical limitations.Therefore,it is crucial to model the thermophysiological responses at the individual level.This study proposed an improved thermo-cardiovascular regulation model(TCRM)by modifying the calculation of blood resistance and incorporating individual height,weight,and age-specific physiological parameters,including the basal metabolic rate,sweat threshold temperature,vascular compliance,blood distribution,and capillary resistance.The model predicts skin and core temperature,blood pressure,and regional blood flow in three participants(25,52,71 years)during non-uniform bathing(half-body immersion:lower body in hot water to chest level,upper body in room air).Evaluation using root mean square error(RMSE)and the coefficient of determination(R2)showed that the model accurately reproduced individual thermophysiological responses.Despite the limited sample,this proof-of-concept study demonstrates that the age-adapted TCRM can capture individual variations in skin temperature,core temperature,blood pressure,and blood flow under non-uniform thermal conditions.This work provides a methodological foundation for larger,prospective validations.展开更多
The Loess Plateau,located in Gansu Province,is an important energy base in China because most of the oil and gas resources are distributed in Gansu Province.In the last 40 a,ecological environment in this region has b...The Loess Plateau,located in Gansu Province,is an important energy base in China because most of the oil and gas resources are distributed in Gansu Province.In the last 40 a,ecological environment in this region has been extremely destroyed due to the over-exploitation of crude-oil resources.Remediation of crude-oil contaminated soil in this area remains to be a challenging task.In this study,in order to elucidate the effects of organic compost and biochar on phytoremediation of crude-oil contaminated soil(20 g/kg)by Calendula officinalis,we designed five treatments,i.e.,natural attenuation(CK),planted C.officinalis only(P),planted C.officinalis with biochar amendment(PB),planted C.officinalis with organic compost amendment(PC),and planted C.officinalis with co-amendment of biochar and organic compost(PBC).After 152 d of cultivation,total petroleum hydrocarbons(TPH)removal rates of CK,P,PB,PC and PBC were 6.36%,50.08%,39.58%,73.10%and 59.87%,respectively.Shoot and root dry weights of C.officinalis significantly increased by 172.31%and 80.96%under PC and 311.61%and 145.43%under PBC,respectively as compared with P(P<0.05).Total chlorophyll contents in leaves of C.officinalis under P,PC and PBC significantly increased by 77.36%,125.50%and 79.80%,respectively(P<0.05)as compared with PB.Physical-chemical characteristics and enzymatic activity of soil in different treatments were also assessed.The highest total N,total P,available N,available P and SOM(soil organic matter)occurred in PC,followed by PBC(P<0.05).C.officinalis rhizospheric soil dehydrogenase(DHA)and polyphenol oxidase(PPO)activities in PB were lower than those of other treatments(P<0.05).The values of ACE(abundance-based coverage estimators)and Chao 1 indices for rhizospheric bacteria were the highest under PC followed by PBC,P,PB and CK(P<0.05).However,the Shannon index for bacteria was the highest under PC and PBC,followed by P,PB and CK(P<0.05).In terms of soil microbial community composition,Proteiniphilum,Immundisolibacteraceae and Solimonadaceae were relatively more abundant under PC and PBC.Relative abundances of Pseudallescheria,Ochroconis,Fusarium,Sarocladium,Podospora,Apodus,Pyrenochaetopsis and Schizothecium under PC and PBC were higher,while relative abundances of Gliomastix,Aspergillus and Alternaria were lower under PC and PBC.As per the nonmetric multidimensional scaling(NMDS)analysis,application of organic compost significantly promoted soil N and P contents,shoot length,root vitality,chlorophyll ratio,total chlorophyll,abundance and diversity of rhizospheric soil microbial community in C.officinalis.A high p H value and lower soil N and P contents induced by biochar,altered C.officinalis rhizospheric soil microbial community composition,which might have restrained its phytoremediation efficiency.The results suggest that organic compost-assisted C.officinalis phytoremediation for crude-oil contaminated soil was highly effective in the Loess Plateau,China.展开更多
The effects of wheat gluten hydrolysates (WGH) and their ethanol elution fractions obtained on XAD-16 resin on physiological activity and fermentation performance of brewer’s yeast during very-high-gravity (VHG) wort...The effects of wheat gluten hydrolysates (WGH) and their ethanol elution fractions obtained on XAD-16 resin on physiological activity and fermentation performance of brewer’s yeast during very-high-gravity (VHG) worts fermentation were investigated.The results showed that the addition of WGH and their elution fractions in VHG worts significantly enhanced yeast biomass and viability,and further increased the fermentability,ethanol yield and productivity of yeast.Supplementation with 40% ethanol fraction exhibited the highest biomass (6.9 g/L dry cell),cell viability,fermentability (82.05%),ethanol titer (12.19%,v/v) and ethanol productivity during VHG worts fermentation.In addition,40% ethanol fraction supplementation also caused the most consumption of amino acid and the highest accumulation of intracellular glycerol and trehalose,15.39% of increase in cell-membrane integrity,39.61% of enhancement in mitochondrial membrane potential (MMP),and 18.94% of reduction in intracellular reactive oxygen species (ROS) level in yeast under VHG conditions.Therefore,WGH supplementation was an efficient method to improve fermentation performance of brewer’s yeast during VHG worts.展开更多
Forest trees can establish symbiotic associations with dark septate endophytes(DSEs)and ectomycorrhizal fungi(ECMF)simultaneously.However,the combined effects of these two fungi on the growth and cadmium(Cd)tolerance ...Forest trees can establish symbiotic associations with dark septate endophytes(DSEs)and ectomycorrhizal fungi(ECMF)simultaneously.However,the combined effects of these two fungi on the growth and cadmium(Cd)tolerance of host plants remain largely unexplored.To address this knowledge gap,a pot experiment was conducted to examine the effects of the interaction between an ECMF strain(Suillus granulatus)and a DSE strain(Pseudopyrenochaeta sp.)on Pinus tabulaeformis under Cd stress,by assessing plant growth and physiological parameters,nutrient uptake,and soil properties.Notably,the colonization rates of both fungal strains were found to increase in response to Cd stress,with the extent of this increase being influenced by the specific fungal species and the Cd level in the soil.Compared to the non-inoculation treatment,single inoculation with fungal strain resulted in enhanced biomass,root development,and nutrient contents in P.tabulaeformis seedlings under Cd stress.Furthermore,a synergistic effect was observed when these seedlings were co-inoculated with S.granulatus and Pseudopyrenochaeta sp.,as indicated by significantly greater measurements in various indicators compared to both the single and non-inoculation treatments.Fungal inoculation effectively regulated the antioxidant defense responses and photosynthesis of P.tabulaeformis seedlings subjected to Cd stress,particularly in the co-inoculation treatment.In addition,fungal inoculation facilitated the Cd accumulation in P.tabulaeformis,suggesting a promising potential for the implementation of bioremediation strategies in the areas contaminated with heavy metals.The findings from this study indicate that the utilization of root symbiotic fungi obtained from stress environments could potentially enhance the growth performance and tolerance of P.tabulaeformis towards heavy metals,and co-inoculation of both fungal groups may result in even more pronounced synergistic effects on the overall fitness of the plant.展开更多
摘要Objective:The objective of this study was to compare the effect of nurse and beloved family member’s recording voice on consciousness and physical parameters in patients with coma state.Materials and Methods:A randomized control trial parallel group design was conducted among 45 comatose patients divided into two intervention groups,i.e.nurse voice stimulus group,receiving nurses voice with standard care,family members voice stimulus group receiving their beloved family member voice with standard care and one control group receiving only standard care in medicine intensive care unit.The intervention was provided three times a day,each lasting 5 min for 7 days in addition to standard care.Repeated measure analysis of variance and independent t-test were used to compare within and between groups,respectively.Results:The study found significant differences in Glasgow coma scale(GCS)scores within both the nurse(F=2.78,P=0.042)and family member(F=10.27,P=0.0001)voice stimulus groups over 7 days.Comparing GCS scores between intervention groups showed significant variations before(P=0.028),during(P=0.047),and after(P=0.036)the intervention on day 7.Comparing GCS scores between the family members’voice stimulus group and the control group,significant changes were observed on days 5 and 7(P=0.043,0.030,0.030,and 0.014,0.012,0.012)before,during,and after the intervention.Conclusions:The use of beloved family members’voices proved more effective in elevating the patients’level of consciousness compared to both the nurse voice stimulus group and the control group.
基金The National Key Technology R&D Program in the 11th Five Year Plan of China under contract No. 2007BAD29B01-2National Department Public Benefit Research Foundation under contract No. nyhyzx 07-048Guangdong Marine and Fishery Bureau under contract Nos A200708C01, A200908A02 and A200908A05
摘要In May of 2007,the second generation selected (SS) and control (SC) groups of pearl oyster Pinctada martensii were established by selecting 10% breeders with the largest and mean shell length,respectively,from the first generation selected group.Growth performance of the SS and SC groups were compared on the basis of measurement data at Days 8,18,60,95,195 and 365.On Day 365,100 individuals (60.0–75.0 mm at shell length) were sampled from each group and then subjected to the experiment where physiological parameters such as filtrate rate,oxygen consumption and ammonia excretion were measured at 15,20,25 and 30°C.The results show that the SS group had significantly larger mean shell length and shell height than the SC group at Days 8,18,60,95,195 and 365 (P 0.05).The genetic gains at different ages varied from 6.0% to 17.0% for shell length and 5.7% to 14.6% for shell height,respectively.At 15,20,25 and 30 ° C,the SS groups had significantly larger filtrate rate than the SC group (P 0.05).At 15 and 25 °C,the differences in oxygen consumption rate between the SS and SC groups were not significant (P 0.05).At 20 and 30 °C,however,the oxygen consumption rate of the SS group was significantly larger than the SC group (P 0.05).At 15,20,25 and 30 °C,there were no significant differences in ammonia excretion rate between the SS and SC groups (P 0.05).The present results indicate that there existed considerable genetic variability in the base population and a further selection could be likely fruitful.Mass selection for faster growth might produce animals that had higher intake of metabolizable energy by virtue of faster filtrating behavior.
基金Supported by the Special Agricultural Project of Agricultural Department (200903003)the Agricultural Modernization Project in the Science and Technology Development Plan of Jilin Province (2009-2010) the High-yield Project of Science and Technology Department (2011BAD16B10)
摘要The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings (chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81) under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.
基金Supported by Special Fund for the Construction of Modern Agricultural IndustryTechnology System(CARS-49)
摘要[ Objective] This study aimed to investigate the effects of 1-naphthol on primary productivity in aquatic ecosystems. [ Method] The effects of 1-naphthol on biomass, chlorophyll a content, malondialdehyde (MDA) content and soluble protein content of Chlorella vulgaris were investigated. [ Result] 1-Naphthol could significantly inhibit the growth of Chlorella vulgaris. On the 7^th d, the biomass of Chlorella vulgaris exposed to 1.0, 3.0, 6.0, 12.0 and 18.0 mg/L 1-naphthol was 60.26%, 48.15%, 41.32%, 28.59% and 27.10% of that in control respectively, exhibiting a significant dose-response relationship. Chlorella vulgaris was exposed to different concentrations of 1-naphthol (0, 1,5 and 10 mg/L) for 7 d ; with the increase of 1-naphthol concentration, chlorophyll a content of Ch/orella vulgaris was reduced, MDA content increased gradually, soluble protein content increased first and then declined. [ Conclusion] According to the experimental results, 1-naphthol can significantly inhibit the growth of Chlorella vulgaris, thus reducing primary productivity in aquatic ecosystems.
基金The authors sincerely thank the deputy of research and technology affiliated to Shiraz University of Medical Sciences for financial support from this study,in the form of a research project by Mr.Hossein Molaeifar approved by the University on No.10652.
摘要Exposure to sound,heat,and increased physical workload can change physiological parameters.This study was conducted to evaluate the effect of concomitant exposure to sound,heat,and physical workload changes on physiological parameters in controlled laboratory conditions.This experimental crosssectional study was conducted in 35 male university students with a mean age of 25.75 years and a mean BMI of 22.69 kg/m2.Systolic and diastolic blood pressure and heart rate were measured after 15 min rest in the laboratory,5 and 10 min after starting the experiment,and then after 20 min in controlled laboratory conditions in five combination modes.The combination modes were(Sound:65 dB,WBGT:22°C,Speed:1.7,Slope:10%),(Sound:65 dB,WBGT:22°C,Speed:3.4,Slope:14%),(Sound:95 dB,WBGT:22°C,Speed:1.7,Slope:10%),(Sound:65 dB,WBGT:32°C,Speed:1.7,Slope:10%),and(Sound:95 dB,WBGT:32°C,Speed:3.4,Slope:14%).Mixed model analysis and paired t-test were applied for analysis.The results showed that the mean physiological parameters(Systolic and diastolic blood pressure and heart rate)increased when different combination modes worsened(Sound from 65 to 95 dB,WBGT from 22°C to 32°C,speed from 1.7 to 3.4,and slope from 10%to 14%,and when sound:95 dB,WBGT:32°C,Speed:3.4,and Slope:14%).Moreover,the mean changes of systolic and diastolic blood pressure were significant in all conditions when compared with the reference condition(Sound:65 dB,WBGT:22°C,Speed:1.7,and Slope:10%).The mean heart rate changes were also significant except for exposure to the second condition(Sound:65 dB,WBGT:22°C,Speed:3.4,Slope:14%)and the third condition(Sound:95 dB,WBGT:22°C,Speed:1.7,Slope:10%).Exposure to hazardous levels of sound,heat,and workload has adverse effects on physiological parameters.Concomitant exposure to all three hazards has a synergistic effect and increases the adverse effect.
基金Supported by Science and Technology Innovation Team Project of Universities in Heilongjiang Province&Graduate Academic Innovation Project of Mudanjiang Normal University
摘要The study was conducted to investigate the characteristics of blood physiological parameters of Apodemus agrarius (A. agrarius). Blood physiological pa- rammers of 10 A. agrarius (half male and half female) were determined by automated hematology analyzer. Results showed that difference of A. agrarius hematokrit (HCT) between male and female was significant (P 〈 0.05 ), and differences of all the other blood physiological parameters between male and female were not sig- nificant ( P 〉 0.05 ). In the study, blood physiological reference value of A. agrarius was set up, which provided a basis for the bio-research and prophylaxis and treatment of A. agrarius.
基金Supported by Sichuan Provincial Mutton Sheep Innovation Team(SCCXTD-2024-14).
摘要[Objectives]This study was conducted to investigate the characteristics of physiological,hematological and blood biochemical parameters in introduced Hu sheep reared in the Zizhong area of Sichuan Province in spring.[Methods]Ten healthy adult rams and ten healthy adult ewes aged 3-5 years were selected,and relevant parameters were measured.[Results]No significant differences were observed between rams and ewes in body temperature,respiratory rate,and heart rate(P>0.05).Among hematological parameters,except for a significantly higher total platelet count in ewes(P0.05).Among blood biochemical parameters,blood urea nitrogen(BUN),alanine aminotransferase(ALT),and the urea nitrogen-to-creatinine ratio were significantly higher in rams than in ewes(P0.05)were observed between rams and ewes in 13 indicators,including total protein,albumin,glucose,and creatine kinase(CK).Overall,the physiological,hematological,and blood biochemical parameters of Hu sheep under housed conditions in the Zizhong area remained stable,with only a few indicators showing significant differences between sexes.This pattern reflects subtle physiological regulatory differences in metabolism and immune function between rams and ewes.[Conclusions]This study provides fundamental data for understanding the physiological adaptability of Hu sheep in the region.
基金Supported by Sichuan Provincial Mutton Sheep Innovation Team(SCCXTD-2024-14).
摘要[Objectives]This study was conducted to evaluate the physiological adaptability of housed Hu sheep introduced to the Zizhong area of Sichuan Province under high-temperature summer conditions.[Methods]Adult male and female Hu sheep(10 per group)reared in a housing system in this region were assessed by measuring physiological,hematological and blood biochemical parameters.[Results]The body temperature of rams was significantly higher than that of ewes(P0.05).Ewes exhibited significantly higher values than rams in several blood physiological parameters,including total leukocyte count,lymphocyte count,hemoglobin content,hematocrit,and mean corpuscular hemoglobin content(P<0.05).No significant differences were detected in the remaining six blood physiological parameters between rams and ewes.Regarding blood biochemical indicators,ewes showed significantly elevated levels of albumin,cholesterol,alkaline phosphatase,and the blood urea nitrogen/creatinine ratio(P<0.05),while rams had significantly higher creatinine content(P<0.05).No significant differences were found between sexes in other 11 blood biochemical indicators,including total protein,globulin,and glucose.These findings suggest that under summer conditions in the Zizhong area,housed Hu sheep exhibit sex-based differences in physiological adaptability,with ewes displaying enhanced blood oxygen-carrying capacity and immune-related indicators compared to rams.[Conclusions]The study provides valuable insights for understanding the adaptation mechanisms of Hu sheep and developing gender-specific management strategies during subtropical summers.
基金Supported by National GMO Cultivation of New Varieties of Major Projects Subproject Environmental Safety Assessment of Genetically Modified Pigs Technology (2008ZX08011-004)~~
摘要[Objective] The aim was to evaluate the health of transgenic sFat-1 gene pigs in order to provide a reference for the breeding selection of pig excellent individuals. [Method] 15 physiological biochemical parameters and reproductive performances of 21 negative control pigs and 20 transgenic sFat-1 gene pigs were determined. [Result] The liver functions of some transgenic sFat-1 gene pigs were hurted lightly,and the reproductive performance also decreased lightly. [Conclusion] The determination of physiological biochemical parameters and reproductive performance in transgenic pigs could be taken as one of effective methods in environmental safety evaluation of transgenic pigs.
摘要Split-root solution culture was used to study the promoting effect of lanthanum on rice (Oryza sativa) growth and its physiological mechanisms. Results sho w that low concentration (0.05~1.5 mg·L -1) increases rice yield an d grain numbers. High concentration depresses grain formation (9~30 mg·L -1 ) and root elongation (1.5~30 mg·L -1). No significant influence on str aw dry weight was found over the whole concentration range except the 0.05 mg·L -1 treatment. With the increase of La concentration from 0.05 to 0.75 mg· L -1, catalase (CAT) activity in the first fully expandeing leaves and root s decreases. When La concentration is greater than 0.75 mg·L -1 or less than 9 mg·L -1, it significantly decreases superoxide dismutase activity ( SOD) in the leaves and roots. No significant effects were found on chlorophyll, protein and malondialdehyde (MDA) content. Possible mechanisms of La′s promotin g effect on rice growth and reduction effect of ·O- 2 were discussed.
基金funded by the National Key Research and Development Program of China(2018YFD0100600)the Special Project for Construction of Modern Agricultural Industrial Technology System(CARS-12).
摘要Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed(Brassica napus L.)varieties.The present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes,P287(drought-tolerant)and T88(drought-sensitive)under three intensities of drought stress.All physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress conditions.At the fourth-leaf stage,the relative water content,chlorophyll content,protein content,malondialdehyde content,and the activities of peroxidase and catalase in P287 were significantly higher than those in T88,particularly under severe drought conditions.After rehydration,all physiological indexes recovered rapidly,especially in P287.In addition,under drought stress,compared with T88,P287 had thicker palisade tissue,thinner spongy tissue,higher ratio of chloroplast length to chloroplast width,higher stomatal density and stomatal closure rate.Overall,the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions.
摘要In Algeria,the factors which contribute to the extension of the phenomenon of land salinization are linked to aridity and the climate which affects>95%of the territory,and the poor quality of irrigation water,to the system of drainage often non-existent.or non-functional,our work in this context and shed light on the effects of salt stress on a sensitive legume.Our study aims to evaluate the effect of salt stress on physiological parameters,and the mycorrhizal activity of Phaseolus vulgaris L,Coco Rose variety and soil analyzes were also carried out.The latter are grown in pots subjected to salt stress with increasing concentrations of Nacl solution of 6%,9%and 12%.Our results confirm that saline stress induces an effect on the parameters studied to varying degrees compared to the control.However,a low content of Chlorophyll A,protein and sugars was recorded and an increase in the proline content as well as a negative influence on the physicochemical parameters of the soil each time the concentration increased.However,on mycorrhizal activity,the results showed a decrease in the colonization rate each time the“Nacl”concentration increased between 80,60 and 30%respectively for decreasing Nacl concentrations.
摘要The objective of this work was to determine the effect of transport time (up to 11 hours) on animal welfare. 540 animals (cows, bulls and calves), three transport times of 2, 4 to 6, and 10 to 11 hours, and two space allowances (2 m2 and 1.5 m2 per animal for cows and bulls; and 1.2 m2 and 0.8 m2 per animal for calves) were used for the experiment during transport from farms to the abattoir in Uppsala, Sweden. Measurements were made on five animals on each trip. Stress response parameters considered were cortisol, glucose, lactate, creatine kinase and heart rate. Blood samples were taken before and after transport. Heart rate sensors were mounted on the animals at least 30 minutes before loading and measurements were made continuously from farms to the abattoirs until stunning. The results of heart rate measurement indicated that loading, un-loading and forced movement in the stunning box were the most stressful events. However, the results of statistical analysis confirmed that transport time doesn't have significant effect (P 〈 0.05) on heart rate. Concentration level of cortisol increased by 10 folds during short transport. However, cortisol concentration decreased with an increase of transport times (P 〈 0.01). Glucose concentrations increased with transport time in all animal categories (P ≤ 0.01). Transport time has significant effect on concentration levels of glucose (P ≤ 0.01), creatine kinase (P 〈 0.001) and lactate (P 〈 0.01) particularly after 6 hours journey time.
基金funded by the National Key Research and Development Program of China(2016YFC0400206-04,2017YFC0505304)the National Natural Science Foundation of China(51309016)+1 种基金Central Public-interest Scientific Institution Basal Research Fund(CKSF2016007/TB)Changjiang River Scientific Research Institute Innovation Team(CKSF2017064/NS)
摘要Exploring the response differences of leaf physiology parameters to enhanced nitrogen deposition between saplings and trees is vital for predicting the variations of terrestrial ecosystem structure and function under future global climate change. In this study, the ecophysiological parameters of saplings and trees of Fraxinus mandshurica Rupr. were measured at different levels of nitrogen addition in a temperate forest. The results show that ecophysiological parameters maximum net photosynthetic rate(Pmax), apparent quantum efficiency(a), dark respiration(Rd), light saturation point(Lsp), photosynthetic nitrogen use efficiency(PNUE),specific leaf area(SLA)and stomatal conductance under saturated light intensity(Gsmax) were higher in saplings than in trees. These physiological parameters and not Nleaf(leaf nitrogen content)led to relatively lower Pmaxand Rdin trees. For both saplings and trees, low and median nitrogen addition(23 and 46 kg ha-1a-1) resulted in significant increases in Pmax, Rd, Lsp, Chl, PNUE, SLA and Gsmax. These parameters tended to decline under high additions of nitrogen(69 kg ha-1a-1),whereas Nleaf was always enhanced with increasing nitrogen. Variations in Pmax and Rd with increasing nitrogen were attributed to variations in the strongly related parameters of, Lsp, Chl, PNUE, SLA and Gsmax. Overall, the response sensitivity of physiological parameters to enhanced nitrogen levels was lower in trees compared with saplings.
摘要This study used non-invasive evaluation methods measured six skin physiological parameters of the lower lip in 180 subjects,including moisture content,transepidermal water loss(TEWL),smoothness(SESM),scaliness of the skin(SESC),wrinkles(SEW),and red area of the lip skin,and compared the effects of 6 groups of lip balms(no-additive group,marine oligosaccharides group,ceramides group,glycyrrhizinic acid group,allantoin group,and mixed group;30 each)on the skin physiological parameters of dry,flaking,and cracked lip subjects.The results showed that the lip mositure content of the subjects in the marine oligosaccharide group,glycyrrhetinic acid group,and allantoin group increased significantly by 44.40%,42.84%and 58.08%after 7 days of lip balm(P<0.05).The TEWL in the ceramide group and the allantoin group was significantly reduced by 21.83%and 24.72%,respectively,after 7 days of lip paste use(P<0.05).The lip skin smoothness values of subjects in the glycyrrhizic acid group and the allantoin group were significantly reduced by 18.76%and 14.97%,respectively,after 28 days of lip balm application(P<0.05).The lip skin scaling indices of subjects in the marine oligosaccharide group,the ceramides group,and the allantoin group were significantly reduced by 33.77%,42.69%,and 38.07%,respectively,after 28 days of lip balm application(P<0.05).The wrinkle parameters of the lip skin of the subjects in the marine oligosaccharide,glycyrrhizinic acid and allantoin groups were significantly reduced by 23.06%,23.29%and 25.98%,respectively,after 28 days of lip balm application(P<0.05).And the area of the red zone of the lip skin of the subjects in the allantoin group was significantly reduced by 4.27%,after 28 days of lip balm application(P<0.05).Combining the effects of the four active ingredients on the secretion of hyaluronic acid(moisturizing effect)and inflammatory factor(IL-6)in HSF cells,it suggests that marine oligosaccharides and allantoin have a perfect impact on enhancing the water content of the skin on the lips of the subjects,and further improve the symptoms of flaking and wrinkles on the lips of the subjects.The ceramide and allantoin can repair the skin barrier well and have a good effect on the chapped and flaky lips of the subjects.After 28 days of using lip balm,the water content of lips in the mixed group increased,the skin barrier was repaired and became smoother,and the wrinkles,scale index,and red zone value were reduced,which could well relieve chronic lip inflammation and lay a foundation for developing lip products for the treatment of chronic cheilitis.
基金supported by JSPS KAKENHI(grant number 22H00737)awarded to T.K.
摘要Among all animals,dogs were domesticated the earliest and formed a unique bond with humans.Hare and Tomasello proposed that in ancestral canids,selection for individuals with reduced fear and aggression allowed them to coexist in a niche adjacent to the human niche(Hare and Tomasello 2005).As a byproduct of this process,dogs acquired social cognitive abilities that facilitated communication with humans.
基金supported by the Japan Society for the Promotion of Science,KAKENHI,Japan[Grant Number JP18H01595].
摘要Many individuals face health risks owing to altered thermophysiological responses in non-uniform indoor thermal environments(e.g.,bathing),with cardiovascular disorders being a significant concern,particularly among older adults.Experimental studies alone are insufficient to fully comprehend individual physiological responses and develop preventive strategies due to ethical and practical limitations.Therefore,it is crucial to model the thermophysiological responses at the individual level.This study proposed an improved thermo-cardiovascular regulation model(TCRM)by modifying the calculation of blood resistance and incorporating individual height,weight,and age-specific physiological parameters,including the basal metabolic rate,sweat threshold temperature,vascular compliance,blood distribution,and capillary resistance.The model predicts skin and core temperature,blood pressure,and regional blood flow in three participants(25,52,71 years)during non-uniform bathing(half-body immersion:lower body in hot water to chest level,upper body in room air).Evaluation using root mean square error(RMSE)and the coefficient of determination(R2)showed that the model accurately reproduced individual thermophysiological responses.Despite the limited sample,this proof-of-concept study demonstrates that the age-adapted TCRM can capture individual variations in skin temperature,core temperature,blood pressure,and blood flow under non-uniform thermal conditions.This work provides a methodological foundation for larger,prospective validations.
基金funded by the Scientific Project of Gansu Province,China(20JR5RA548)the National Natural Science Foundation of China(31860148)+1 种基金the Natural Science Foundation of Gansu Province,China(21JR1RM333)the Doctoral Program of Longdong University,China(XYBY1909)。
摘要The Loess Plateau,located in Gansu Province,is an important energy base in China because most of the oil and gas resources are distributed in Gansu Province.In the last 40 a,ecological environment in this region has been extremely destroyed due to the over-exploitation of crude-oil resources.Remediation of crude-oil contaminated soil in this area remains to be a challenging task.In this study,in order to elucidate the effects of organic compost and biochar on phytoremediation of crude-oil contaminated soil(20 g/kg)by Calendula officinalis,we designed five treatments,i.e.,natural attenuation(CK),planted C.officinalis only(P),planted C.officinalis with biochar amendment(PB),planted C.officinalis with organic compost amendment(PC),and planted C.officinalis with co-amendment of biochar and organic compost(PBC).After 152 d of cultivation,total petroleum hydrocarbons(TPH)removal rates of CK,P,PB,PC and PBC were 6.36%,50.08%,39.58%,73.10%and 59.87%,respectively.Shoot and root dry weights of C.officinalis significantly increased by 172.31%and 80.96%under PC and 311.61%and 145.43%under PBC,respectively as compared with P(P<0.05).Total chlorophyll contents in leaves of C.officinalis under P,PC and PBC significantly increased by 77.36%,125.50%and 79.80%,respectively(P<0.05)as compared with PB.Physical-chemical characteristics and enzymatic activity of soil in different treatments were also assessed.The highest total N,total P,available N,available P and SOM(soil organic matter)occurred in PC,followed by PBC(P<0.05).C.officinalis rhizospheric soil dehydrogenase(DHA)and polyphenol oxidase(PPO)activities in PB were lower than those of other treatments(P<0.05).The values of ACE(abundance-based coverage estimators)and Chao 1 indices for rhizospheric bacteria were the highest under PC followed by PBC,P,PB and CK(P<0.05).However,the Shannon index for bacteria was the highest under PC and PBC,followed by P,PB and CK(P<0.05).In terms of soil microbial community composition,Proteiniphilum,Immundisolibacteraceae and Solimonadaceae were relatively more abundant under PC and PBC.Relative abundances of Pseudallescheria,Ochroconis,Fusarium,Sarocladium,Podospora,Apodus,Pyrenochaetopsis and Schizothecium under PC and PBC were higher,while relative abundances of Gliomastix,Aspergillus and Alternaria were lower under PC and PBC.As per the nonmetric multidimensional scaling(NMDS)analysis,application of organic compost significantly promoted soil N and P contents,shoot length,root vitality,chlorophyll ratio,total chlorophyll,abundance and diversity of rhizospheric soil microbial community in C.officinalis.A high p H value and lower soil N and P contents induced by biochar,altered C.officinalis rhizospheric soil microbial community composition,which might have restrained its phytoremediation efficiency.The results suggest that organic compost-assisted C.officinalis phytoremediation for crude-oil contaminated soil was highly effective in the Loess Plateau,China.
基金supported by the National Natural Science Foundation of China(Nos.31972062 and 32001675)the Science and Technology Project of Guangzhou(201903010056)+2 种基金the Science and Technology Project of Guangdong Province(Nos.2018A050506008 and 2016A010105003)the 111 Project(B17018)the Scientific Research Foundation for High-Level Talents in Southwest Minzu University.
摘要The effects of wheat gluten hydrolysates (WGH) and their ethanol elution fractions obtained on XAD-16 resin on physiological activity and fermentation performance of brewer’s yeast during very-high-gravity (VHG) worts fermentation were investigated.The results showed that the addition of WGH and their elution fractions in VHG worts significantly enhanced yeast biomass and viability,and further increased the fermentability,ethanol yield and productivity of yeast.Supplementation with 40% ethanol fraction exhibited the highest biomass (6.9 g/L dry cell),cell viability,fermentability (82.05%),ethanol titer (12.19%,v/v) and ethanol productivity during VHG worts fermentation.In addition,40% ethanol fraction supplementation also caused the most consumption of amino acid and the highest accumulation of intracellular glycerol and trehalose,15.39% of increase in cell-membrane integrity,39.61% of enhancement in mitochondrial membrane potential (MMP),and 18.94% of reduction in intracellular reactive oxygen species (ROS) level in yeast under VHG conditions.Therefore,WGH supplementation was an efficient method to improve fermentation performance of brewer’s yeast during VHG worts.
基金funded by the National Natural Science Foundation of China(No.32001112)the Natural Science Foundation of Hebei Province,China(Nos.C2020204169 and C2023204238)。
摘要Forest trees can establish symbiotic associations with dark septate endophytes(DSEs)and ectomycorrhizal fungi(ECMF)simultaneously.However,the combined effects of these two fungi on the growth and cadmium(Cd)tolerance of host plants remain largely unexplored.To address this knowledge gap,a pot experiment was conducted to examine the effects of the interaction between an ECMF strain(Suillus granulatus)and a DSE strain(Pseudopyrenochaeta sp.)on Pinus tabulaeformis under Cd stress,by assessing plant growth and physiological parameters,nutrient uptake,and soil properties.Notably,the colonization rates of both fungal strains were found to increase in response to Cd stress,with the extent of this increase being influenced by the specific fungal species and the Cd level in the soil.Compared to the non-inoculation treatment,single inoculation with fungal strain resulted in enhanced biomass,root development,and nutrient contents in P.tabulaeformis seedlings under Cd stress.Furthermore,a synergistic effect was observed when these seedlings were co-inoculated with S.granulatus and Pseudopyrenochaeta sp.,as indicated by significantly greater measurements in various indicators compared to both the single and non-inoculation treatments.Fungal inoculation effectively regulated the antioxidant defense responses and photosynthesis of P.tabulaeformis seedlings subjected to Cd stress,particularly in the co-inoculation treatment.In addition,fungal inoculation facilitated the Cd accumulation in P.tabulaeformis,suggesting a promising potential for the implementation of bioremediation strategies in the areas contaminated with heavy metals.The findings from this study indicate that the utilization of root symbiotic fungi obtained from stress environments could potentially enhance the growth performance and tolerance of P.tabulaeformis towards heavy metals,and co-inoculation of both fungal groups may result in even more pronounced synergistic effects on the overall fitness of the plant.