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Dietary supplementation with sodium isobutyrate enhances growth performance and colonic barrier function in weaned piglets via microbiota-metabolite-host interactions 认领 引用 被引量：1
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作者 Xiuyu Fang Zihan Chi +6 位作者 Zhengyi Wang Xinlin Wang Xingrui Qu Shuang Zhang Feng Gao Baoming Shi Xuan Zhao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期404-425,共22页
Background Weaning-induced diarrhoea and growth retardation in piglets are associated with impaired intestinal barrier function and decreased levels of colonic short-chain fatty acids(SCFAs).Although SCFA supplementat... Background Weaning-induced diarrhoea and growth retardation in piglets are associated with impaired intestinal barrier function and decreased levels of colonic short-chain fatty acids(SCFAs).Although SCFA supplementation has been proposed to mitigate these issues,the efficacy and optimal dosage of sodium isobutyrate remain unclear.Results We investigated the effects of sodium isobutyrate supplementation(500,1,000,2,000,and 4,000 mg/kg diet)on weaned piglets(Duroc×Landrace×Yorkshire,28 d of age;n=8).After a 28-d feeding trial,supplementation at 500–2,000 mg/kg significantly improved average daily gain and feed efficiency and reduced diarrhoea frequency,with maximal benefits observed at 1,000 mg/kg(P<0.0001).Additionally,500–1,000 mg/kg sodium isobutyrate supplementation increased the apparent digestibility of crude protein,organic matter,and crude fibre(P<0.05).Serum biochemical parameters were unaffected,although secretory immunoglobulin A(SIgA)levels significantly increased upon supplementation with 500–1,000 mg/kg(P<0.05).16S rRNA gene sequencing indicated that sodium isobutyrate increased the abundance of beneficial colonic microbiota.The 1,000 mg/kg group presented the most pronounced effect,with a significant increase of the relative abundance of Prevotella and the greatest improvement in SCFA concentrations(P<0.05).Metabolomics revealed elevated levels of colonic indole-3-lactic acid and 3-hydroxybutyrate upon supplementation with 1,000 mg/kg(P<0.05).Transcriptomic analyses indicated activation of protein digestion and absorption pathways,and PI3K-Akt signalling,marked by TSG-6 upregulation and the suppression of ISG15 and DDIT4 expression(P<0.05).Supplementation with 1,000 mg/kg was associated with improved intestinal barrier-related markers,including reduced serum D-lactate,diamine oxidase,and lipopolysaccharide levels,increased tight junction protein expression;activation of G protein-coupled receptors;and inhibition of TLR4/MyD88/NF-κB signalling(P<0.05),suggesting enhanced barrier function.Conclusions In conclusion,dietary supplementation with 1,000 mg/kg sodium isobutyrate was associated with improved intestinal morphology,reduced serum permeability,increased expression of tight junction proteins,and enhanced immune function in weaned piglets,suggesting enhanced colonic barrier function and providing dosage guidance and mechanistic insights for future applications. 展开更多
关键词 Colonic barrier function Diarrhoea Intestinal microbiota Sodium isobutyrate Weaned piglets
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Lactate alleviates intestinal barrier injury in weaned piglets via activation of the Wnt/β-catenin pathway and promotion of intestinal epithelial cell proliferation 认领 引用
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作者 Mingyu Wang Yifan Chen +10 位作者 Jiaojiao Chen Aimin Wu Daiwen Chen Bing Yu Jun He Jie Yu Xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Ping Zheng 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期443-456,共14页
Background Inflammatory bowel disease causes intestinal structural damage,impairs gut function,hinders animal growth and development,and reduces farming efficiency.Previous studies demonstrated that lactate alleviates... Background Inflammatory bowel disease causes intestinal structural damage,impairs gut function,hinders animal growth and development,and reduces farming efficiency.Previous studies demonstrated that lactate alleviates dextran sulfate sodium(DSS)-induced inflammation and mitigates weight loss by enhancing intestinal barrier functions.However,the mechanisms underlying lactate-mediated protection of the intestinal epithelial barrier remain unclear.This study aimed to explore the protective effect of lactate on intestinal barrier damage in colitis piglets and the possible underlying mechanisms through in vivo and in vitro experiments.Methods A total of 6021-day-old weaned female piglets were randomly assigned into three groups based on weight:the control group(basal diet with physiological saline gavage),the DSS group(basal diet with 5%DSS gavage),and the DSS+LA group(2%lactate diet with 5%DSS gavage).There were 10 replicates per treatment,with 2 piglets per replicate.Jejunal morphology was assessed via hematoxylin and eosin staining,while Western blotting quantified the protein levels of proliferation markers,including cluster of differentiation 24(CD24),cyclin D1,and wingless/integrated(Wnt)/β-catenin signaling components.In vitro,0.08%DSS and 2–32 mmol/L sodium lactate-treated intestinal porcine epithelial cell line-J2(IPEC-J2)cells(n=4)were assessed for viability(Cell Counting Kit-8 assay),apoptosis(flow cytometry),and proliferation parameters,including cell cycle analysis and Leucine-rich repeat-containing G-protein coupled receptor 5(Lgr5+)stem cell quantification.Results In vivo,DSS administration induced jejunal villus shortening(P<0.05),downregulated protein levels of CD24,cyclin D1,casein kinase 1(CK1),and dishevelled-2(DVL2)(P<0.05).In vitro,DSS promoted apoptosis,inhibited proliferation,diminished the Lgr5+cell populations(P<0.05),and reduced S-phase cell proportions(P<0.05).Conversely,lactate supplementation ameliorated DSS-induced villus atrophy(P<0.05),restored CD24,cyclin D1,CK1,and DVL2 protein levels(P<0.05).Furthermore,in vitro,sodium lactate attenuated DSS-induced apoptosis(P<0.05),enhanced IPEC-J2 proliferation(P<0.05),expanded Lgr5+cells(P<0.05),and increased S-phase progression(P<0.05).Conclusions In summary,lactate ameliorated intestinal barrier damage in DSS-induced colitis by activating the Wnt/β-catenin pathway and restoring the balance between epithelial cell proliferation and apoptosis.This study provides novel mechanistic evidence supporting lactate's therapeutic potential for IBD management. 展开更多
关键词 Apoptosis Intestinal inflammation Lactate Lgr5 Piglets Proliferation Wnt/β-catenin pathway
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Integrative clustering by colonic volatile fatty acids reveals microbiota and host physiological variation in weaned piglets 认领 引用
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作者 Chengcheng Li Stijn Heirbaut +5 位作者 Joris Michiels Yuhuang Hou Anneke Ovyn Noémie Van Noten Elout Van Liefferinge Jeroen Degroote 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第3期1554-1572,共19页
Background Volatile fatty acids(VFAs)reflect microbial fermentation linking gut microbiota,metabolism and host physiology.However,endogenous VFA profiles and their physiological relevance remain insufficiently charact... Background Volatile fatty acids(VFAs)reflect microbial fermentation linking gut microbiota,metabolism and host physiology.However,endogenous VFA profiles and their physiological relevance remain insufficiently characterized.This study aimed to identify distinct VFA phenotypes in weaned piglets and reveal how microbial fermentation patterns connect gut metabolism with animal health and performance.Results In this study,we integrated six independent trials comprising 164 weaned piglets.Here,k-means clustering of six VFAs in colonic digesta to identified two metabolite phenotypes:high level of short-chain fatty acids(HSCFA)and high level of branched-chain fatty acids(HBCFA).Generally,The HSCFA group exhibited significantly higher concentrations of VFA(P<0.001),and lower levels of ammonia and serum urea(P<0.001),consistent with enhanced carbohydrate fermentation and reduced nitrogen output.Additionally,the HSCFA group had significantly greater villus height in the distal small intestine(P<0.01),lower mid-colonic pH(P<0.001)and thrombocyte counts(P<0.01).In contrast,the HBCFA group showed elevated levels of branched-chain fatty acids and ammonia(P<0.001),as well as enrichment of Escherichia-Shigella,Rikenellaceae dgA-11 gut group and Prevotella(adjusted P<0.05).The HSCFA group was enriched in Lactobacillus,Mitsuokella and Dialister(adjusted P<0.05),and exhibited higher final body weight and average daily gain(P<0.05),indicating better growth performance.To explore associations between gut microbiota and VFA phenotypes,an XGBoost classification model was trained,based on genus-level composition,to predict the metabolic phenotype.It achieved a moderate accuracy and identified Unclassified Eubacterium coprostanoligenes group,Lactobacillus and Escherichia-Shigella as important genera contributing to group separation.Conclusions Clustering based on colonic VFA profiles identified distinct microbial and physiological patterns that may influence gut function and growth performance in weaned piglets.The enrichment of beneficial microbes and fermentation products in the HSCFA group suggests a more favorable intestinal environment,while protein fermentation in the colon seems associated with reduced animal performance and less beneficial intestinal environment. 展开更多
关键词 Branched-chain fatty acids Microbiota Short-chain fatty acids Weaned piglets
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Multi-omic analysis for dietary supplementation of different ratios of soluble and insoluble fiber on intestinal microbiota,metabolites and inflammation of weaned piglets 认领 引用
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作者 Yetong Xu Chengyu Zhou +7 位作者 Yingying Lu Xutong Guo Minyue Zong Junwei Zhu Pan Zhou Jiaman Pang Xie Peng Zhihong Sun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第4期1606-1618,共13页
Different types of dietary fiber(DF)play important roles in enhancing intestinal health and overall performance in animals.This study investigated the effects of high-DF diets containing different ratios of soluble to... Different types of dietary fiber(DF)play important roles in enhancing intestinal health and overall performance in animals.This study investigated the effects of high-DF diets containing different ratios of soluble to insoluble dietary fiber(SDF:IDF)on growth performance,intestinal barrier integrity,microbiota,and metabolite profiles in weaned piglets.The four dietary treatments consisted of a basal diet(CON)and three high-DF diets with SDF:IDF ratios of 0.37,0.25,and 0.13(designated HF-0.37,HF-0.25,and HF-0.13,respectively).On days 14 and 28,colonic tumor necrosis factor-α,interleukin-1β,interleukin-6,and interleukin-8 concentrations were higher in the HF-0.37 group than in the CON,HF-0.25,and HF-0.13 groups(P<0.05).Plasma D-lactate and endotoxin levels were lower in the HF-0.25 group compared to the CON group at both time points(P<0.05).Furthermore,colonic zonula occludens 1 expression was upregulated in the HF-0.25 and HF-0.13 groups compared to the CON and HF-0.37 groups on day 14(P<0.05).At the transcriptional level,all three high-DF diets modulated signaling pathways associated with inflammation and immune responses in the colon.Notably,DF supplementation particularly the HF-0.25 diet upregulated colonic levels of 3-indole butyric acid,nicotinic acid,and 3-methylthiopropylamine on d 14 and reduced certain peptide levels by d 28.These findings indicate that DF supplementation,especially at an SDF:IDF ratio of 0.25,exerts beneficial effects on intestinal integrity in weaned piglets,potentially mediated by alterations in colonic metabolite profiles,whereas HF-0.37 and HF-0.13 exhibited limited impacts on intestinal barrier function. 展开更多
关键词 weaned piglets soluble fiber insoluble fiber multi-omics intestinal integrity
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Strategically isolated bacteriophages targeting ETEC K88(F4)alleviate post-weaning diarrhea in piglets via modulation of gut microbiota and inflammatory responses 认领 引用 被引量：1
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作者 Yan Chen Minfeng Ding +4 位作者 Xingping Chen Tiande Zou Yi Liu Jun Chen Jinming You 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期1080-1101,共22页
Background Post-weaning diarrhea(PWD)in piglets,primarily caused by enterotoxigenic Escherichia coli(ETEC)K88(F4)infection,presents a major challenge in swine production.This study aimed to isolate bacteriophages(phag... Background Post-weaning diarrhea(PWD)in piglets,primarily caused by enterotoxigenic Escherichia coli(ETEC)K88(F4)infection,presents a major challenge in swine production.This study aimed to isolate bacteriophages(phages)specific to ETEC K88,utilizing ETEC K88 as the host strain,and to assess the efficacy of dietary supplementation with the isolated phages in weaned piglets over a two-week period using an ETEC K88 challenge model in a pilot study.Results Three ETEC K88-specific phages(EC-P1,EC-P2,and EC-P3)were isolated and identified as tailed phages.These phages displayed a short latency period,broad acid–base stability,and thermal stability,effectively inhibiting ETEC K88 growth and disrupting ETEC K88 biofilms in vitro.Lyophilized phage powder was prepared and supplemented at 400,600 or 800 mg/kg in the diets.Compared to the ETEC K88 group,piglets in the ETEC K88+600 or 800 mg/kg phages group exhibited markedly lower diarrhea scores and rectal temperatures at 12,24,and 48 h post-infection.Supplementation with 600 mg/kg phages enhanced intestinal integrity of ETEC K88-infected piglets,as evidenced by an increased jejunal villus height and villus height-to-crypt depth ratio,reduced serum diamine oxidase and D-lactate levels,and upregulated jejunal ZO-1 protein expression.Concomitantly,systemic and jejunal inflammatory responses were attenuated by supplementation with 600 mg/kg of phages,as evidenced by decreased serum LPS,IL-1β,IL-10 and TNF-α levels,down-regulated jejunal IL-1β and IL-6 mRNA expression,and suppressed NF-κB signalling(downregulated p-IκBα/IκBα and p-p65/p65 ratios).Supplementation with 600 mg/kg phages also shifted the faecal microbiota toward eubiosis,increasing the Shannon index,decreasing Proteobacteria and Enterobacteriaceae abundances,and elevating beneficial taxa(Patescibacteria,Muribaculaceae,and Subdoligranulum).Correlation analysis further revealed that Proteobacteria and Enterobacteriaceae abundances were positively associated with diarrhoea characteristics,whereas Muribaculaceae showed a negative correlation.Conclusions Three ETEC K88-targeting phages were successfully isolated,characterized,and prepared as lyophilized phage powder for dietary supplementation.Dietary supplementation with 600 mg/kg of lyophilized phage powder alleviated PWD in piglets by modulating gut microbiota and inflammatory responses. 展开更多
关键词 Bacteriophages ETEC K88 Gut microbiota Inflammation Post-weaning diarrhea Weaned piglets
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Fecal microbiota transplantation mitigates lipopolysaccharide-induced oxidative stress in weaned piglets by modulating gut microbiota and enhancing riboflavin metabolism 认领 引用 被引量：1
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作者 Jixiang Ma Mengqi Liu +3 位作者 Junying Xu Boshuai Liu Yalei Cui Yinghua Shi 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期1002-1019,共18页
Background During the weaning phase,piglets are exposed to significant physiological and environmental stressors,which disrupt the balance of their intestinal microbiota and often lead to severe diarrhea.Previous stud... Background During the weaning phase,piglets are exposed to significant physiological and environmental stressors,which disrupt the balance of their intestinal microbiota and often lead to severe diarrhea.Previous studies have demonstrated that alfalfa fiber,derived from the stems and leaves of alfalfa,can effectively alleviate diarrhea in piglets.Additionally,multiple studies have highlighted the potential of fecal microbiota transplantation(FMT)in mitigating diarrhea in various models of intestinal diseases in young animals.However,the specific mechanisms by which FMT from targeted sources alleviates diarrhea in weaned piglets remain to be fully elucidated.Results In this study,FMT from donor piglets fed an alfalfa fiber-supplemented diet effectively alleviated diarrhea,improved intestinal morphology,and enhanced gut barrier function in weaned piglets.FMT further promoted the colonization of beneficial bacterial genera(including UCG-005,unclassified Lachnospiraceae,Lachnospiraceae AC2044 group,UCG-002,Candidatus Saccharimonas,and Lachnospiraceae ND3007 group)while inhibiting the detrimental genus Tyzzerella,consequently enhancing the production of short-chain fatty acids(SCFAs).Additionally,FMT upregulated riboflavin metabolism,leading to elevated flavin adenine dinucleotide(FAD)levels and increased glutathione reductase activity,thereby collectively attenuating lipopolysaccharide(LPS)-induced oxidative stress and contributing to intestinal health.Conclusions We found that FMT modulates the structure of the gut microbiota,enhances microbial diversity and composition,increases the production of SCFAs,and upregulates riboflavin metabolism to elevate FAD levels.These changes collectively enhance immune and antioxidant capacities,thereby alleviating diarrhea. 展开更多
关键词 Fecal microbiota transplantation Flavin adenine dinucleotide Lipopolysaccharide Oxidative stress Short-chain fatty acids Weaned piglets
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Dietary supplementation with Bacillus-based probiotic improves gut health in the weaned piglets challenged by rotavirus 认领 引用
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作者 Fengyu Xiang Heng Yang +15 位作者 Xiangqi Fan Dayan Tan Bing Huang Bing Yu Jun He Yuheng Luo Junqiu Luo Hui Yan Junning Pu Jianping Wang Quyuan Wang Huifen Wang John Kyaw Htoo Santa Maria Mendoza Guiling Yan Xiangbing Mao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期951-961,共11页
Background As probiotics,Bacillus strains may regulate some physiological functions in animals.This study aimed to evaluate whether dietary supplementation with a Bacillus-based probiotic could alleviate gut damage in... Background As probiotics,Bacillus strains may regulate some physiological functions in animals.This study aimed to evaluate whether dietary supplementation with a Bacillus-based probiotic could alleviate gut damage induced by rotavirus(RV)infection in piglets.Twenty-four piglets were randomly assigned into 2 groups fed with the basal diet(n=16)and the diet containing 109 colony-forming unit Bacillus spores/kg(n=8).On d 8,8 piglets fed with the diet supplemented with Bacillus-based probiotic and 8 piglets fed with basal diet were orally infused with RV,while the residue piglets had oral gavage of sterile essential medium.The trial duration was 12 d.Results RV challenge induced diarrhea,significantly destroyed the morphology of jejunal mucosa(P<0.05),significantly increased RV-antibody and RV non-structural protein 4 of jejunal mucosa(P<0.05),significantly impaired antioxidant capacity(including malondialdehyde level,total antioxidant capacity and catalase activity),immunity(such as interleukin 2,interleukin 4 and secreted immunoglobulin A levels),mucins and the m RNA expression of tightjunction-related(such as Zonula occludens 1,occludin)and apoptotic-related(including B-cell lymphoma/leukaemia-2-associated X protein,B cell lymphoma/leukaemia-2,cysteinyl aspartate specific proteinases)genes of jejunal mucosa(P<0.05),and,to some extents,affected the bacteria community structure and abundance of ileal digesta in piglets.However,Bacillus-based probiotic administration could significantly attenuate the negative effects of RV infection on gut health of piglets(P<0.05).Conclusions These findings suggested that supplementing Bacillus-based probiotic in the diet could decrease diarrhea rate,and improve gut health in weaned piglets,which was associated with regulating intestinal antioxidant capacity,apoptosis,and microbiota. 展开更多
关键词 Apoptosis Antioxidant capacity Bacillus strains Intestinal health Microbiota Rotavirus Weaned piglets
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Butyrate combined with niacin enhances intestinal barrier function repair in weaned piglets infected with ETEC by promoting colonic metabolism and antimicrobial peptide expression 认领 引用
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作者 Qingsong Tang Rui Zhen +9 位作者 Bijing Yang Zhenyan Miao Yangyang Wei Shihui Ruan Yiyi He Yunxia Xiong Qiwen Wu Li Wang Zongyong Jiang Hongbo Yi 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第4期2273-2292,共20页
Background Weaning piglets are highly susceptible to enterotoxigenic Escherichia coli(ETEC)infections,which can cause intestinal barrier function dysfunction and death.However,there is still a lack of efficient,econom... Background Weaning piglets are highly susceptible to enterotoxigenic Escherichia coli(ETEC)infections,which can cause intestinal barrier function dysfunction and death.However,there is still a lack of efficient,economical,and safe nutritional interventions.This study aimed to investigate the effects of combining butyrate with niacin on intestinal barrier function repair and resistance to ETEC infection in weaned piglets.In this study,two 14-d animal experiments were designed to observe the optimal butyrate-to-niacin ratio and assess their responses to the ETEC challenge.Results Supplementation with butyrate and niacin at a ratio of 100:2(2,000 mg/kg butyrate and 40 mg/kg niacin,BN2)increased the average daily gain(ADG)and reduced the diarrhea incidence.We also observed an increase in the levels of nicotinamide adenine dinucleotide(NAD)in the colon of weaned piglets.Notably,BN2 promoted amino acid anabolism in the colon and enhanced glycolysis and the tricarboxylic acid(TCA)cycle by increasing the acetylation of key enzymes in the TCA.Furthermore,BN2 enhanced the expression of indispensable genes for the colonic mucosal barrier,including antimicrobial peptides such as porcineβdefensin 1(pBD1),porcineβdefensin 2(pBD2),and proline-arginine rich 39-amino acid peptide(PR39),tight junction proteins,and improved colonic microbiome composition.Based on these findings,we found that BN2 alleviated growth restriction and diarrhea,and modulated the expression of antimicrobial peptides,tight junction proteins,and cytokines to reduce colonic barrier function dysfunction in weaned piglets challenged with ETEC.Mechanistically,we confirmed that BN2elevated the protein expression of acetylation of histone 3 lysin 27(H3K27ac)and enhanced the binding of acH3K27to the promoter regions of pBD1 and PR39.Conclusions Supplementation with BN2 improved growth performance,supported colonic barrier function repair,and enhanced disease resistance in weaned piglets challenged with ETEC.This offers new insights into nutritional strategies for intestinal barrier function repair of piglets infected with ETEC. 展开更多
关键词 Butyrate Colonic barrier function Enterotoxigenic Escherichia coli Niacin Weaned piglets
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Piceatannol enhances antioxidant capacity and growth in weaned piglets by regulating of Nrf2-mediated redox homeostasis and modulating of the related gut microbiota 认领 引用
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作者 Longlong Zhu Yuyan Che +4 位作者 Meixia Chen Long Cai Qiujue Wu Tao Feng Jing Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期935-950,共16页
Background Piglets are highly susceptible to oxidative stress,which can reduce growth performance and cause intestinal damage.Piceatannol(PIC),a natural bioactive substance enriched in Chinese rhubarb(Rheum officinale... Background Piglets are highly susceptible to oxidative stress,which can reduce growth performance and cause intestinal damage.Piceatannol(PIC),a natural bioactive substance enriched in Chinese rhubarb(Rheum officinale)and certain dark purple fruits,shows excellent antioxidant properties in our previous cell-based high-throughput screening.However,its effect on piglet growth performance and antioxidant capacity as well as underling mechanism has not been thoroughly investigated.Methods One hundred weaned pigs(28 days of age,8.71±0.20 kg)were randomly assigned to 4 treatments with 5 replicates of 5 pigs per replicate.The experimental diets consisted of:1)basal diet,2)basal diet+100 mg/kg PIC,3)basal diet+200 mg/kg PIC,and 4)basal diet+300 mg/kg PIC.On d 15 and 35,one pig from each replicate was selected for sampling.The growth performance was monitored during a 35-day trial.In addition,H2O2-challenged IPEC-J2 cells served as an in vitro model to investigate the antioxidant mechanisms of PIC.IPEC-J2 cells were treated with 1,000μmol/L H2O2in the presence or absence of 10μmol/L PIC.Results Dietary PIC at 200 mg/kg significantly enhanced growth performance,as evidenced by increased average daily gain and feed conversion rate(P<0.05).PIC supplementation markedly improved systemic antioxidant capacity,with elevated serum total antioxidant capacity,catalase activity,and glutathione levels,along with reduced malondialdehyde content(P<0.05).Notably,PIC modulated the gut microbiota composition,increasing the amounts of beneficial genera(e.g.,Blautia and Faecalibacterium),and these microbial shifts significantly correlated with improved antioxidant indices.In vitro,PIC pretreatment effectively protected IPEC-J2 cells against H2O2-induced oxidative damage by reducing reactive oxygen species generation and lipid peroxidation(P<0.01).Mechanistically,PIC exerts its antioxidant effects through Nrf2 pathway activation,upregulating endogenous antioxidant enzymes(P<0.05)while simultaneously inhibiting apoptosis via the regulation of the Bcl-2/Bax ratio and caspase-3 cleavage(P<0.01).Conclusions PIC improved the growth performance and health status of weaned piglets through the regulation of Nrf2-mediated redox homeostasis and modulation of the related gut microbiota,offering a potential new natural antioxidants for mitigating weaning stress in piglets. 展开更多
关键词 Antioxidant Growth performance Intestinal flora Nrf2 pathway Piceatannol Weaning piglets
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Iron deficiency aggravates hepatic inflammation in suckling piglets via endoplasmic reticulum stress-driven NF-κB pathway activation 认领 引用
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作者 Jun Qi Yaxu Liang +8 位作者 Dongming Yu Weite Li Fei Long Meng Yuan Zhangbo Lou Chunxue Liu Gaiqin Wang Bencheng Wu Xiang Zhong 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第3期1712-1728,共17页
Background Iron deficiency(ID)poses a significant health burden to both human infants and suckling piglets.In piglets,ID leads to substantial economic losses for the industry by compromising growth performance,health,... Background Iron deficiency(ID)poses a significant health burden to both human infants and suckling piglets.In piglets,ID leads to substantial economic losses for the industry by compromising growth performance,health,and survival.However,current research has predominantly concentrated on hematological abnormalities,whereas the mechanisms underlying ID-associated hepatic inflammatory injury remain inadequately elucidated.Our study employed the iron-deficient suckling piglet model to address this knowledge gap and to establish a molecular theoretical foundation.Results To investigate the underlying mechanisms,this study conducted in vivo and in vitro models.In piglets,ID triggered hepatic oxidative stress by inducing a redox imbalance and suppressing the core Nrf2/HO-1 antioxidant signaling pathway.Histopathological examination revealed structural abnormalities in ID piglet livers,including disorganized hepatic cords,cytoplasmic vacuolation,hydropic degeneration,and mononuclear inflammatory cell infiltration.Transmission electron microscopy further showed shrunk nuclear envelopes,reduced numbers of rough endoplasmic reticulum(RER),and dilated RER cisternae in hepatocytes of ID piglets.Mechanistically,ID activated endoplasmic reticulum stress(ERS)and the PERK/IRE1α branches of the unfolded protein response(UPR).RNA-seq transcriptomic analysis demonstrated significant dysregulation of immune-related pathways,accompanied by elevated pro-inflammatory cytokines(e.g.,IL1B,TNF)and decreased anti-inflammatory cytokines(e.g.,IL4,IL10).Central to this inflammatory response was the activation of the TLR4/NF-κB pathway,evidenced by upregulation of MyD88 and increased phosphorylation of IκBαand NF-κB p65.In vitro,deferoxamine(DFO)-induced ID in AML12 hepatocytes consistently recapitulated the key features of this phenotype,including the activation of ERS/UPR and the TLR4/NF-κB signaling pathway.Pharmacological inhibition of ERS by 4-phenylbutyric acid(4-PBA)attenuated DFO-induced NF-κB activation and ameliorated the imbalance between pro-and anti-inflammatory cytokines.Conclusions ID exacerbated hepatic inflammation through ERS-mediated activation of the NF-κB pathway,providing novel mechanistic insights into liver injury associated with ID. 展开更多
关键词 Endoplasmic reticulum stress Hepatic inflammation Iron deficiency Piglet TLR4/NF-κB pathway Unfolded protein response
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The gut microbial metabolite indole‑3‑aldehyde alleviates impaired intestinal development by promoting intestinal stem cell expansion in weaned piglets 认领 引用 被引量：6
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作者 Jiaqi Zhang Yahui Chen +5 位作者 Xin Guo Xuan Li Ruofan Zhang Mengting Wang Weiyun Zhu Kaifan Yu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期455-470,共16页
Background Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbio-sis.However,it has been challenging to clarify which specific intestinal microbiota and their metabolites ... Background Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbio-sis.However,it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets.Results In this study,we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score,and elevated levels of lipopolysaccharide(LPS)and D-lactate(D-LA)compared to healthy piglets.Subsequently,we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets.Diarrheal piglets demonstrated intestinal microbiota dysbiosis,characterized pri-marily by a higher Firmicutes to Bacteroidota ratio,a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri,and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron.Functional pro-filing of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes(KEGG)data was performed,and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diar-rhea.Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets.Furthermore,we explored the effects of dietary indole-3-aldehyde(IAld),a key tryptophan metabolite,on intestinal development and gut barrier function in weaned piglets.Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell(ISC)expansion in piglets.The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids.Conclusions These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intesti-nal development by promoting ISC expansion in weaned piglets. 展开更多
关键词 Indole-3-aldehyde Intestinal development Intestinal microbiota Intestinal stem cell Weaned piglets
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Baicalin alleviates intestinal inflammation and microbial disturbances by regulating Th17/Treg balance and enhancing Lactobacillus colonization in piglets 认领 引用 被引量：5
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作者 Shunfen Zhang Chengzeng Luo +6 位作者 Kai Li Junhong Wang Huixin Wang Ruqing Zhong Liang Chen Qiugang Ma Hongfu Zhang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第2期866-882,共17页
Background Intestinal inflammation is a common and serious health problem in piglet production,especially enteritis caused by pathogenic Escherichia coli(E.coli).This condition often leads to high mortality,slow weigh... Background Intestinal inflammation is a common and serious health problem in piglet production,especially enteritis caused by pathogenic Escherichia coli(E.coli).This condition often leads to high mortality,slow weight gain,and significant economic losses.Results In this study,we isolated an E.coli strain,SKLAN202302,from the colon of diarrheal piglets to create an intestinal inflammation model for evaluating the protective effects of baicalin.Piglets infected with E.coli exhibited significant reductions in body weight,feed intake,small intestine length,and ileal goblet cell count(P<0.05),along with deteriorated ileal morphology.However,baicalin supplementation resulted in body weights,feed intake,and intestinal morphology similar to those of the control group.Notably,there was a significant increase in the colonization of Lactobacillus species,particularly Lactobacillus_reuteri,Lactobacillus_amylovorus,and Lactobacillus_johnii,compared to the E.coli group(P<0.05).At the metabolic and transcriptional levels,E.coli infection increased inflammatory mediators,including eicosanoids(leukotriene F4,prostaglandin F1a,leukotriene E4,thromboxane B2,prostaglandin G2,and PGH2),monosaccharides,and TCA cycle intermediates(oxoglutaric acid,glutaric acid,adipic acid,citric acid,and isocitric acid)in the ileum.It also promoted the expression of genes related to autoimmune diseases and the Th17 differentiation signaling pathway(CTLA4,IFN-ALPHA-8,IL12RB2,TRAV3,TRAV16,FOS,and VEGFA),as well as inflammatory factors.Conversely,baicalin supplementation not only counteracted these effects but also enhanced the presence of metabolites such as phospholipids[including lyso PC(P-18:1(9Z)/0:0),PC(17:0/0:0),lyso PC(16:1(9Z)/0:0),PC(18:0/0:0),lyso PC(18:0/0:0),PA(10:0/i-16:0),and PA(10:0/8:0)]and amino acids.It also regulated genes within the IL-17 signaling pathway(IL4,CCL17,CXCL10,IFNG,and CXCL2),suggesting a mechanism by which baicalin mitigates E.coli-induced intestinal and microbial disturbances.Subsequent flow cytometry analysis showed that E.coli infection increased the numbers of CD3+and Foxp3+cells,decreased IL-17A+cells,and reduced Th17/Treg ratios.Baicalin supplementation restored these parameters to control levels.Conclusions Baicalin supplementation effectively alleviates E.coli-induced intestinal inflammation and microbial disturbances in piglets by enhancing beneficial Lactobacillus colonization,counteracting inflammatory mediators,and regulating immune-related gene expression and the Th17/Treg balance.These findings highlight baicalin's potential in alleviating intestinal inflammation. 展开更多
关键词 Baicalin Escherichia coli Intestinal inflammation Microbiota Piglets Th17/Treg balance Th17 cell
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Dietary ferulic acid supplementation enhances antioxidant capacity and alleviates hepatocyte pyroptosis in diquat challenged piglets 认领 引用 被引量：3
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作者 Junqiu Luo Xiu Wu +2 位作者 Daiwen Chen Bing Yu Jun He 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期377-387,共11页
Background Oxidative stress significantly impacts growth performance and liver function in piglets.Ferulic acid(FA)works as an antioxidant,however,the role and mechanism of FA in the regulation of diquat-induced oxida... Background Oxidative stress significantly impacts growth performance and liver function in piglets.Ferulic acid(FA)works as an antioxidant,however,the role and mechanism of FA in the regulation of diquat-induced oxidative stress in piglets are less known.This study was designed to investigate the effects of FA on growth performance and antioxi-dant capacity in piglets with diquat challenge.Methods Thirty-two healthy DLY(Duroc×Landrace×Yorkshire)piglets(13.24±0.19 kg)were randomly divided into one of two diets including 0 or 4 g/kg FA for 14 d.On d 15,all pigs were intraperitoneally injected diquat or sterile saline.Results Dietary supplementation with ferulic acid(FA)significantly improved the average daily gain(ADG)and decreased feed-gain ratio(F/G)of piglets.Here,dietary FA supplementation reduced serum aspartate aminotrans-ferase(AST),alanine aminotransferase(ALT)activities in diquat challenged piglets.Furthermore,diquat infusion increased reactive oxygen radicals(ROS)level in liver,decreased the activities of total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC)and increased malondialdehyde(MDA)con-tent in the liver and serum.Supplementation with FA significantly increased T-AOC and T-SOD activities and decreased MDA and ROS levels.FA down-regulated gene and protein expression of Keap1,and up-regulated protein expression of Nrf2 and HO-1 in the liver of piglets with diquat challenge.Importantly,diquat challenge increased the ratio of late apoptosis,increased serum levels of IL-1β,IL-18 and lactate dehydrogenase(LDH),and up-regulated pyroptosis-related genes in the liver.FA supplementation reduced the ratio of late apoptosis and down-regulated mRNA expression of Caspase-1.Accordingly,FA addition reduced concentration of IL-1β,IL-18,and LDH under diquat challenge.Conclusions Diquat-induced oxidative stress reduced growth performance and impaired liver function in piglets.Dietary FA supplementation enhanced the antioxidant capacity and reduced the degree of hepatocyte pyroptosis,thereby alleviating the oxidative damage in the liver and mitigating the impact of diquat on growth performance of piglets. 展开更多
关键词 Antioxidant capacity Ferulic acid Hepatic pyroptosis Piglets
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Taurine alleviated paraquat-induced oxidative stress and gut-liver axis damage in weaned piglets by regulating the Nrf2/Keap1 and TLR4/NF-κB signaling pathways 认领 引用
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作者 Chen Chen Min Qi +7 位作者 Weilong Zhang Fanxing Chen Zhihong Sun Weizhong Sun Wenjie Tang Zhenguo Yang Xuan Zhao Zhiru Tang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第6期2852-2874,共23页
Background Oxidative stress can impair intestinal barrier function and cause liver damage,resulting in reduced animal productivity.Paraquat(PQ)induces significant oxidative stress in weaned piglets.The antioxidant,ant... Background Oxidative stress can impair intestinal barrier function and cause liver damage,resulting in reduced animal productivity.Paraquat(PQ)induces significant oxidative stress in weaned piglets.The antioxidant,anti-inflammatory,and metabolic regulatory functions of taurine(Tau),a free amino acid that is widely distributed in the body,have been extensively studied.However,the mechanisms by which dietary Tau alleviates oxidative stress and gut-liver axis damage in weaned piglets remain unclear.Methods Forty weaned piglets(20 males and 20 females;6.41±0.11 kg;25 days old;Duroc×Landrace×Yorkshire)were used in a 2×2 factorial design to investigate the mechanism by which dietary Tau(0%or 0.4%)alleviates PQ-induced oxidative stress and gut-liver axis damage.We analyzed key biomarkers related to gut barrier function,mucosal damage repair,liver damage,gut-liver immunity,antioxidant capacity,systemic immune homeostasis,antioxidant levels,and gut microbiota diversity in piglets under normal and acute oxidative stress.In particular,we evaluated the coordinated regulation of gut-liver axis function mediated by Tau through the Nrf2/Keap1(antioxidant)and TLR4/NF-κB(immune modulation)signaling pathways.Partial least squares path modeling and molecular docking were used to explore the intrinsic relationship between PQ,Tau,and the gut-liver axis.Results PQ exposure impaired gut barrier function,increased the liver fibrosis area,and markedly affected gut microbial diversity(P<0.05).Tau effectively alleviated PQ-induced oxidative stress by activating the Nrf2/Keap1 pathway and inhibiting the TLR4/NF-κB pathway.This enhanced gut barrier function,promoted mucosal repair,and significantly suppressed the concentration and circulation of lipopolysaccharides in the blood,consequently reducing liver damage(P<0.05).This further facilitated the optimization of gut microbiota composition,thereby supporting the positive regulation of the gut-liver axis and improving systemic immune and antioxidant functions.Conclusions Tau improved the health status of weaned piglets under both normal and stressed conditions by modulating the Nrf2/Keap1 and TLR4/NF-κB pathways,offering a potential new nutritional strategy for alleviating gut-liver damage. 展开更多
关键词 Antioxidant Gut-liver axis Immune response Intestinal barrier Lipopolysaccharide Paraquat Taurine Weaned piglets
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Multi‑omics profiling reveals Poria cocos polysaccharides mitigate PEDV‑induced intestinal injury by modulating lipid metabolism in piglets 认领 引用
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作者 Qian Zhang Shuaijie Wang +6 位作者 Mengjun Wu Zihan Tan Tao Wu Dan Yi Lei Wang Di Zhao Yongqing Hou 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2267-2280,共14页
Background Porcine epidemic diarrhea virus(PEDV)infection poses a significant challenge to the swine industry,with limited effective control measures available.Poria cocos polysaccharides(PCP)is the primary active ing... Background Porcine epidemic diarrhea virus(PEDV)infection poses a significant challenge to the swine industry,with limited effective control measures available.Poria cocos polysaccharides(PCP)is the primary active ingredient of Poria cocos,and has been demonstrated to show beneficial effects on intestinal damage in previous studies.However,its mechanism has not been fully understood.In the present study,18 seven-day-old piglets were divided into 3 groups:Control group,PEDV group,and PCP+PEDV group.After three days of adaptation,piglets in the PCP+PEDV group were orally administered 10 mg/kg body weight/d PCP from d 4 to 10.On d 8,piglets were orally administered with PEDV at the dose of 104.5 TCID50/piglet.This study aimed to investigate the potential effects of PCP on PEDVinduced intestinal injury and explored the underlying mechanisms.Results The results showed that PCP administration effectively alleviated diarrhea,reduced PEDV replication in the small intestine and colon of piglets,and significantly improved intestinal mucosal morphology.Specifically,PCP increased the villus height in both the jejunum and ileum and increased the villus height to crypt depth ratio in the ileum(P<0.05).Improved intestinal function was further evidenced by elevated plasma D-xylose levels and decreased diamine oxidase activity(P<0.05).Transcriptomic and proteomic analyses revealed that lipid metabolism is a key pathway regulated by PCP during PEDV infection.Notably,PCP significantly upregulated sphingolipid metabolism-related genes,including ectonucleotide pyrophosphatase/phosphodiesterase family member 7 and N-acylsphingosine amidohydrolase 2.Metabolomic analysis revealed that PCP primarily modulated the levels of plasmanylphosphoethanolamine,lysophosphatidylcholine,and carnitine.Additionally,PCP reversed the expression of key genes involved in fatty acid uptake,intracellular lipid transport,and fatty acid synthesis,such as fatty acid binding protein 2,fatty acid transport protein 4,apolipoprotein B,apolipoprotein C3,fatty acid synthase,long-chain fatty acyl CoA synthetase 3,lipoprotein lipase and acyl-CoA thioesterases 12(P<0.05).Conclusions These findings demonstrate that PCP mitigates PEDV-induced intestinal injury by modulating lipid metabolism and highlight its potential as a dietary supplement for enhancing anti-PEDV defenses and promoting intestinal health in piglets. 展开更多
关键词 Intestinal injury Lipid metabolism Piglets Porcine epidemic diarrhea virus Poria cocos polysaccharides
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Grass hay mixed‑in creep feed or separately‑fed differentially affects digestive development in pre‑and post‑weaning piglets 认领 引用
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作者 Renjie Yao Tetske G.Hulshof +4 位作者 Hubèrt M.J.van Hees An Cools Mattijs Merckx Dominiek Maes Geert P.J.Janssens 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2302-2321,共20页
Background Based on observations in feral pigs,the role of dietary fibre and structure may be underestimated in suckling piglet nutrition.This study investigated the effect of grass hay offered to suckling piglets eit... Background Based on observations in feral pigs,the role of dietary fibre and structure may be underestimated in suckling piglet nutrition.This study investigated the effect of grass hay offered to suckling piglets either separately or included in their creep feed,combined with nursery diets with or without grass pellet inclusion on growth performance and gastrointestinal development.Methods Thirty-six litters(14–15 piglets per litter)were divided into three equal groups of 12 litters per treatment during the suckling phase:control group(CON)received regular creep feed;GH group received chopped grass hay as-is in separate feeders alongside regular creep feed;PGH group received regular creep feed but barley and wheat were replaced by 28%grass pellets.After weaning(d 23),each litter was split into two dietary treatments in a splitplot design(pre-wean treatment as main plot).Two of the pre-wean diets were also offered until d 14 post-weaning,i.e.,CON(CON nursery diet,CON-C,GH-C,PGH-C)and PGH(GH nursery diet,CON-GH,GH-GH,PGH-GH).Thereafter,transitioning to a diet containing 13%wheat/barley or grass pellets,respectively,until d 39 post-weaning.Gastrointestinal morphology,gene expression of intestinal nutrient transporters and barrier proteins,metabolite profile and microbiota were assessed on the day before weaning,d 10 and d 38 post-weaning.A total of 24 piglets were sacrificed at each dissection point.Results At weaning,GH group had consumed 7 g/piglet grass hay,and PGH group had consumed 46 g/piglet creep feed.One day before weaning,GH piglets showed heavier emptied small intestine(P=0.044)and colon(P=0.065),higher SCFA production in proximal segments and lower SCFA production in colon(P<0.05).Higher abundance of Prevotellaceae NK3b31 group was observed in caecal and colonic content of PGH compared to GH group(P<0.05),and PGH group showed a lower energy conversion ratio(net energy intake/gain,P=0.035).Following weaning,GH nursery group had a reduced average daily gain(226 vs.183 g,P<0.001)during d 0–14,while this group showed compensatory growth afterwards(P=0.056).Main plot effects on increased expressions of CLDN3 and FFAR2 were observed in GH and PGH by d 38 post-weaning(P<0.05).An interaction effect showed greater luminal abundance of the Prevotellaceae NK3b31 group in GH-GH and PGH-GH groups compared to CON-GH on d 38.The GH nursery diet showed a better energy conversion ratio(P=0.006)with no influence on body weight and their SCFA production shifted towards proximal segments.Conclusion In conclusion, feeding a structured and fibre-rich diet to suckling piglets enhance their digestive tractdevelopment and adapt their microbiome to fibre digestion in later life. Maintaining a fibre-rich diet from sucklingto nursery is recommended, though this come with a transient reduction in weight gain caused by lower feed intakethat, however, can be recovered afterwards accompanied with an optimized energy conversion ratio. 展开更多
关键词 Dietary fibre Gastrointestinal development Grass hay Suckling piglets
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Gut microbiota-mediated bile acid transformations regulate the transport of aflatoxin B1 from the intestine to the liver in piglets 认领 引用 被引量：2
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作者 Jiangdi Mao Yusen Wei +3 位作者 Zhixiang Ni Jinzhi Zhang Junli Zhu Haifeng Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第4期1732-1749,共18页
Background Aflatoxins have been reported as a significant pollutant in feed,capable of causing harm to the liver,gastrointestinal tract and kidneys of piglets.However,research on the interactions among aflatoxin B1(AF... Background Aflatoxins have been reported as a significant pollutant in feed,capable of causing harm to the liver,gastrointestinal tract and kidneys of piglets.However,research on the interactions among aflatoxin B1(AFB1),bile acid(BA)metabolism and gut microbiota is limited.Methods In this study,piglets were treated with AFB1 and antibiotics(ABX)to evaluate the interaction between AFB1 and gut microbiota.Subsequently,the roles of the farnesoid X receptor(FXR)and sterol 12α-hydroxylase(CYP8B1)in AFB1 absorption were studied by using FXR agonists obeticholic acid(OCA)and Cyp8b1-knockout(KO)mice,respectively.Result AFB1 inhibited bile salt hydrolase(BSH)activity in ileal microbiota,downregulated ileal FXR expression,and upregulated CYP8B1 expression in liver,increasing the proportion of 12α-OH BAs and potentially enhancing AFB1 absorption.ABX treatment reduced AFB1 absorption and liver damage,and unexpectedly increased BSH activity,counteracting the AFB1-induced downregulation of FXR and upregulation of CYP8B1.OCA reactivated ileal FXR,reduced AFB1 absorption,and alleviated liver damage.Furthermore,Cyp8b1-KO mice showed increased resistance to AFB1-induced liver damage by lowering AFB1 absorption.Conclusions These results underscore the significance of gut microbiota and BAs in AFB1 absorption,suggesting new strategies to mitigate health risks from AFB1 in piglets. 展开更多
关键词 Aflatoxin Bile acid CYP8B1 FXR Microbiota Piglet
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Bacillus velezensis MZ09 alleviates DSS-induced colitis in piglets by remodeling the intestinal microbiota activating the SCFAs–GPR43–STAT3 pathway and suppressing NLRP3 inflammasome-mediated pyroptosis 认领 引用 被引量：2
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作者 Zhengyi Wang Xiuyu Fang +4 位作者 Zhihua Yu Xiangyu Huo Haiyang Liu Yongqing Du Baoming Shi 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第6期2944-2965,共22页
Background Inflammatory bowel disease(IBD)is closely associated with intestinal microbiota dysbiosis and metabolic dysfunction.The aim of this study was to explore the protective effects and mechanisms of the probioti... Background Inflammatory bowel disease(IBD)is closely associated with intestinal microbiota dysbiosis and metabolic dysfunction.The aim of this study was to explore the protective effects and mechanisms of the probiotic Bacillus velezensis MZ09,which produces branched-chain short-chain fatty acids(BSCFAs),against the dextran sulfate sodium(DSS)-induced colitis in piglets.Results In this study,a DSS-induced piglet colitis model was established to explore the impact of MZ09.Pretreatment with MZ09 significantly alleviated the symptoms of colitis in piglets.For example,the disease activity index(DAI)score decreased,the length of the colon was restored,and splenomegaly was alleviated.MZ09 enhanced intestinal barrier integrity by upregulating the expression of tight junction proteins such as Claudin-1,Occludin,and ZO-1.Using 16S rRNA analysis,we found that MZ09 could remodel the intestinal microbiota.MZ09 increased the abundance of beneficial bacteria such as Firmicutes and Lactobacillus while suppressing the growth of harmful bacteria such as Proteobacteria and Escherichia-Shigella.MZ09 also increased the levels of short-chain fatty acids(SCFAs)in the colon.The increased SCFA content activated G-protein-coupled receptor 43(GPR43),which increased the phosphorylation of signal transducer and activator of transcription 3(STAT3)and promoted the production of the antiinflammatory cytokine interleukin-10(IL-10).Mechanistically,MZ09 mitigated mitochondrial damage via the STAT3/hypoxia-inducible factor 1α(HIF-1α)axis.This action inhibits nucleotide-binding oligomerization domain,leucinerich repeat and pyrin domain-containing 3(NLRP3)inflammasome-mediated pyroptosis,thus reducing the release of the proinflammatory cytokines IL-1βand IL-18.Conclusions B.velezensis MZ09 alleviates DSS-induced colitis in piglets through multiple pathways,including gut microbiota remodeling,SCFAs–GPR43–STAT3 axis activation,and NLRP3 inflammasome-mediated pyroptosis suppression.These findings provide a new theoretical basis for the development of targeted intervention strategies for IBD,suggesting that MZ09 represents a potentially promising therapeutic agent for IBD treatment. 展开更多
关键词 Bacillus velezensis BSCFAs Disease resistance GPR43 Piglet Pyroptosis STAT3
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Dietary resveratrol attenuation of intestinal inflammation and oxidative damage is linked to the alteration of gut microbiota and butyrate in piglets challenged with deoxynivalenol 认领 引用 被引量：32
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作者 Yueqin Qiu Jun Yang +4 位作者 Li Wang Xuefen Yang Kaiguo Gao Cui Zhu Zongyong Jiang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第4期1555-1571,共17页
Background:Deoxynivalenol(DON)is a widespread mycotoxin that induces intestinal inflammation and oxidative stress in humans and animals.Resveratrol(RES)effectively exerts anti-inflammatory and antioxidant effects.Howe... Background:Deoxynivalenol(DON)is a widespread mycotoxin that induces intestinal inflammation and oxidative stress in humans and animals.Resveratrol(RES)effectively exerts anti-inflammatory and antioxidant effects.However,the protective effects of RES on alleviating DON toxicity in piglets and the underlying mechanism remain unclear.Therefore,this study aimed to investigate the effect of RES on growth performance,gut health and the gut microbiota in DON-challenged piglets.A total of 64 weaned piglets[Duroc×(Landrace×Yorkshire),21-d-old,6.97±0.10 kg body weight(BW)]were randomly allocated to 4 treatment groups(8 replicate pens per treatment,each pen containing 2 males;n=16 per treatment)for 28 d.The piglets were fed a control diet(CON)or the CON diet supplemented with 300 mg RES/kg diet(RES group),3.8 mg DON/kg diet(DON)or both(DON+RES)in a 2×2 factorial design.Results:DON-challenged piglets fed the RES-supplemented diet had significantly decreased D-lactate concentrations and tumor necrosis factor alpha(TNF-α)and interleukin 1 beta(IL-1β)mRNA and protein expression,and increased zonula occludens-1(ZO-1)mRNA and protein expression compared with those of DON-challenged piglets fed the unsupplemented diet(P<0.05).Compared with unsupplemented DON-challenged piglets,infected piglets fed a diet with RES showed significantly decreased malondialdehyde(MDA)levelsand increased mRNA expression of antioxidant enzymes and antioxidant genes(i.e.,GCLC,GCLM,HO-1,SOD1 and NQO-1)and glutamatecysteine-ligase modulatory subunit(GCLM)protein expression(P<0.05).Moreover,RES supplementation significantly abrogated the increase in the proportion of TUNEL-positive cells and the protein expression of caspase3 in DON-challenged piglets(P<0.05).Finally,RES supplementation significantly increased the abundance of Roseburia and butyrate concentrations,while decreasing the abundances of Bacteroides and unidentified-Enterobacteriaceae in DON-challenged piglets compared with DON-challenged piglets alone(P<0.05).Conclusions:RES supplementation improved gut health in DON-challenged piglets by strengthening intestinal barrier function,alleviating intestinal inflammation and oxidative damage,and positively modulating the gut microbiota.The protective effects of RES on gut health may be linked to increased Roseburia and butyrate concentrations,and decreased levels of Bacteroides and unidentified-Enterobacteriaceae. 展开更多
关键词 Deoxynivalenol Gut health Gut microbiota Inflammation Oxidative damage Piglets Resveratrol
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Dietary protocatechuic acid ameliorates inflammation and up-regulates intestinal tight junction proteins by modulating gut microbiota in LPS-challenged piglets 认领 引用 被引量：40
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作者 Ruizhi Hu Ziyu He +5 位作者 Ming Liu Jijun Tan Hongfu Zhang De-Xing Hou Jianhua He Shusong Wu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第1期328-339,共12页
Background: Weaning is one of the major factors that cause stress and intestinal disease in piglets. Protocatechuic acid(PCA) is an active plant phenolic acid which exists in Chinese herb, Duzhong(Eucommia ulmoides Ol... Background: Weaning is one of the major factors that cause stress and intestinal disease in piglets. Protocatechuic acid(PCA) is an active plant phenolic acid which exists in Chinese herb, Duzhong(Eucommia ulmoides Oliver), and is also considered as the main bioactive metabolite of polyphenol against oxidative stress and inflammation. This study aimed to investigate the effect of PCA on growth performance, intestinal barrier function, and gut microbiota in a weaned piglet model challenged with lipopolysaccharide(LPS).Methods: Thirty-six piglets(Pig Improvement Company line 337 × C48, 28 d of age, 8.87 kg ± 0.11 kg BW) were randomly allocated into 3 treatments and fed with a basal diet(CTL), a diet added 50 mg/kg of aureomycin(AUR), or a diet supplemented with 4000 mg/kg of PCA, respectively. The piglets were challenged with LPS(10 μg/kg BW) on d 14 and d 21 by intraperitoneal injection during the 21-d experiment. Animals(n = 6 from each group) were sacrificed after being anesthetized by sodium pentobarbital at 2 h after the last injection of LPS. The serum was collected for antioxidant indices and inflammatory cytokines analysis, the ileum was harvested for detecting mRNA and protein levels of tight junction proteins by PCR and immunohistochemical staining, and the cecum chyme was collected for intestinal flora analysis using 16 S rRNA gene sequencing.Results: Dietary supplementation of PCA or AUR significantly increased the expression of tight junction proteins including ZO-1 and claudin-1 in intestinal mucosa, and decreased the serum levels of thiobarbituric acid reactive substances(TBARS) and IL-6, as compared with CTL group. In addition, PCA also decreased the serum levels of IL-2 and TNF-α(P < 0.05). Analysis of gut microbiota indicated that PCA increased the Firmicutes/Bacteroidetes ratio(P < 0.05). Spearman's correlation analysis at the genus level revealed that PCA reduced the relative abundance of Prevotella 9, Prevotella 2, Holdemanella, and Ruminococcus torques group(P < 0.05), and increased the relative abundance of Roseburia and Desulfovibrio(P < 0.05), whereas AUR had no significant effect on these bacteria.Conclusions: These results demonstrated that both PCA and AUR had protective effect on oxidative stress, inflammation and intestinal barrier function in piglets challenged with LPS, and PCA potentially exerted the protective function by modulating intestinal flora in a way different from AUR. 展开更多
关键词 Gut microbiota Inflammation Piglets Protocatechuic acid Tight junction proteins
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