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StCIPK2-StCBL11 complex negatively modulates drought tolerance in potato plant by regulating oxidative resistance and ABA biosynthesis 认领 引用 被引量：1
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作者 Rui Ma Weigang Liu +4 位作者 Shigui Li Xi Zhu Jiangwei Yang Ning Zhang Huaijun Si 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第5期1119-1132,共14页
Calcineurin B-like(CBL)proteins and CBL-interacting protein kinases(CIPK)play essential roles in regulating plant responses to various stresses.However,the molecular and functional properties of CBL-CIPK in drought re... Calcineurin B-like(CBL)proteins and CBL-interacting protein kinases(CIPK)play essential roles in regulating plant responses to various stresses.However,the molecular and functional properties of CBL-CIPK in drought response have been rarely characterised in potato plants.In this study,we subjected the potato‘Atlantic’cultivar[wild type(WT),ami-St CIPK2,and overexpression(OE)lines]to stress through ABA treatment,osmotic stress,and drought stress.We characterised the potato CBL-interacting protein kinase StCIPK2.We measured the relative water content,superoxide dismutase and peroxidase activities,and H2O2,proline,malondialdehyde,and soluble sugar levels as these are indices of drought tolerance.We estimated the stomatal movement and root growth phenotype(length of plant roots).Finally,we performed a protein interaction assay.We found that StCIPK2 was effectively induced by multiple stressors and is highly homologous to At CIPK1.Accordingly,St CIPK2 overexpression decreased drought tolerance and ABA sensitivity in potato,whereas StCIPK2knock-down increased these responses.Moreover,StCIPK2 interacted with St CBL11 on the plasma membrane.Additionally,the drought tolerance indices showed that drought resistance and ABA sensitivity did not differ between StCBL11-OE and WT plants,and St CBL11overexpression in StCIPK2 knock-down plants negated their drought resistance and ABA sensitivity.Overall,this study shows that the St CBL11-StCIPK2 module negatively regulates drought stress tolerance in potatoes,and it could be used in novel ways to improve stress regulation in potato plant. 展开更多
关键词 Potato StCIPK2 StCBL11 Drought tolerance Antioxidant defense ABA
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An intracellular Ras-group related leucine-rich repeat protein IbPIRL8 increases soft rot and root rot resistance in sweet potato 认领 引用 被引量：1
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作者 Wenbin Wang Kui Peng +6 位作者 Meiqi Shang Hong Zhai Shaopei Gao Ning Zhao Qingchang Liu Huan Zhang Shaozhen He 《The Crop Journal》 SCIE CSCD 2026年第3期849-858,共10页
The plant intracellular Ras-group related leucine-rich repeat proteins(PIRLs)play important roles in pollen tube growth and development.However,the role of PIRLs in modulating defense responses against fungal diseases... The plant intracellular Ras-group related leucine-rich repeat proteins(PIRLs)play important roles in pollen tube growth and development.However,the role of PIRLs in modulating defense responses against fungal diseases has not been reported.In this study,the IbPIRL8 gene was isolated from the resistant variety Nongdabai.Its expression was strongly induced by salicylic acid(SA)and methyl jasmonate.The IbPIRL8 protein was localized to the nucleus and cell membrane.Overexpression of IbPIRL8 conferred resistance to soft rot and root rot in sweet potato.Key SA-responsive genes,cellulose biosynthesisrelated genes and callose biosynthesis-related genes were upregulated in IbPIRL8-OE plants compared with wild type(WT).Consistently,the storage roots of IbPIRL8-OE plants accumulated more endogenous SA,cellulose and callose than WT plants.Yeast one-hybrid,dual-luciferase,and electrophoretic mobility shift assays demonstrated that IbDEAR2,induced by Rhizopus stolonifer,binds to the IbPIRL8 promoter to repress its expression.These findings provide insights into the genetic basis for improving diseaseresistant sweet potato varieties. 展开更多
关键词 Sweet potato IbPIRL8 IbDEAR2 Soft rot Root rot
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StHEMERA and StPIF4 synergistically regulate thermomorphogenesis in potato(Solanum tuberosum L.) 认领 引用
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作者 Rongrong Liu Wenqian Liang +6 位作者 Lina Shang Xinlong Chen Jirong Yin Jieli Xu Yan Li Dianqiu Lyu Hongju Jian 《The Crop Journal》 SCIE CSCD 2026年第2期436-447,共12页
While high-temperature stress severely inhibits the growth,development,and tuberization of potatoes(Solanum tuberosum L.),moderate high temperatures can trigger thermomorphogenesis.However,the molecular mechanisms und... While high-temperature stress severely inhibits the growth,development,and tuberization of potatoes(Solanum tuberosum L.),moderate high temperatures can trigger thermomorphogenesis.However,the molecular mechanisms underlying thermomorphogenesis in potato remain largely elusive.In this study,we identified a heat-responsive,nucleus-plastid shuttling transcriptional activator,StHEMERA(StHMR),whose nuclear translocation was enhanced under high-temperature conditions.Transgenic analyses revealed that the high-temperature induced increase in plant height and cell elongation in potato depends on the function of StHMR.The StHMR-silenced lines displayed a phenotype insensitive to elevated temperatures.Biochemical evidence further revealed that StHMR forms a regulatory module with PHYTOCHROME INTERACTING FACTOR 4(StPIF4),a potato homolog of the central Arabidopsis thermosensory.StPIF4 is indispensable for high-temperature-induced stem elongation and directly binds the promoters of the auxin biosynthesis gene YUCCA8(StYUC8)and the cell-elongation geneα-EXPANSION A8(StEXPA8).StPIF4 regulates cell elongation under high-temperature conditions by binding to E-box elements within the promoters of these genes.Notably,StHMR functions as a transcriptional co-activator that enhances the ability of StPIF4 to activate the transcription of StYUC8,especially under heat conditions.Collectively,this study characterizes the StHMR-StPIF4 module as a key molecular unit of potato thermomorphogenesis and highlights its potential relevance to tuber formation,providing promising molecular targets for breeding heat-tolerant potato varieties. 展开更多
关键词 Potato High temperature StHEMERA StPIF4 Thermomorphogenesis
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Potato StCERK1 is a key regulator of plant immunity against multiple pathogens 认领 引用
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作者 Lijuan Cao Mingchuan Pan +9 位作者 Langhua Yu Chuntao Lv Dandan Zhao Wei Wu Futing Li Ju Ai Dongli Gao Sanwen Huang Yi Shang Xuming Luo 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第8期1855-1868,共14页
Potato(Solanum tuberosum L.),the world's third-largest crop,faces significant yield losses due to susceptibility to diverse plant pathogens,impacting global food security.Receptor-like kinases(RLKs)activate plant ... Potato(Solanum tuberosum L.),the world's third-largest crop,faces significant yield losses due to susceptibility to diverse plant pathogens,impacting global food security.Receptor-like kinases(RLKs)activate plant immunity by recognizing damage-and pathogen-associated molecular patterns(DAMPs and PAMPs).Among these,chitin elicitor receptor kinase 1(CERK1)is essential for detecting chitin and confers resistance to various pathogens in Arabidopsis thaliana,Oryza sativa,and Solanum lycopersicum.However,generating homozygous mutants in potato is challenging,leaving functions of CERK1 in potato(StCERK1)unexplored.This study identified StCERK1 in potato and applied a tRNA-scaffolded gRNA editing strategy with four gRNAs to edit the StCERK1 gene in tetraploid potato,generating homozygous mutants with high efficiency.We confirmed StCERK1 as a functional kinase and found that it was essential for chitin signal response in potato.It is likely involved in regulating potato immunity through the flavonoid biosynthesis pathway.Furthermore,phenotypic analysis revealed that stcerk1 mutants exhibit increased susceptibility to Phytophthora infestans,Alternaria solani,and Ralstonia solanacearum compared to the wild type.Notably,StCERK1 was also implicated in tuber disease resistance.These findings highlight StCERK1 as a key regulator of potato immunity against multiple pathogens,suggesting potential strategies for broad-spectrum crop resistance against diseases in agricultural production through the utilization of plant immune receptors. 展开更多
关键词 Potato CERK1 Chitin Plant immunity Lysin motif receptor-like kinase
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Effect of Selenium,Copper and Manganese Nanocomposites in Polysaccharide Matrices on the Content of Photosynthetic Pigments in Potato Leaf Tissues 认领 引用
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作者 Alla I.Perfileva Konstantin V.Krutovsky 《Phyton-International Journal of Experimental Botany》 SCIE 2026年第5期100-115,共16页
The article presents the study of the effect of nanocomposites(NCs)based on selenium(Se),copper(Cu),and manganese(Mn)nanoparticles(NPs)embedded in a matrix of natural polysaccharides—arabinogalactan(AG),carrageenan(C... The article presents the study of the effect of nanocomposites(NCs)based on selenium(Se),copper(Cu),and manganese(Mn)nanoparticles(NPs)embedded in a matrix of natural polysaccharides—arabinogalactan(AG),carrageenan(CAR),and starch(ST)—on the content of chlorophylls(Chls)and carotenoids in potato tissues in vitro.Potatoes were grown for 28 days on Murashige-Skoog(MS)medium with the addition of a NC,then pigments were isolated from leaf tissues,and their content was determined spectrophotometrically.Both a stimulating effect and an inhibitory effect of different NCs on the pigment content were found.Se and Cu NCs in the AG matrices(Se/AG and Cu/AG NCs)increased the Chl content both in the leaf tissues of healthy potato plants and plants infected with the phytopathogenic bacterium Clavibacter sepedonicus(Cms).Mn NCs increased the content of carotenoids and Chls in potatoes infected with Cms.A decrease in the pigment content was detected when growing potatoes on a medium with the addition of Cu NC based on a ST matrix(Cu/ST NC),which is probably due to the high content of NPs in this NC(20%).It was concluded that NCs based on an AG matrix are capable of exerting a stimulating effect on the content of potato pigments,which can be used to increase the yield of this crop. 展开更多
关键词 Clavibacter sepedonicus copper manganese nanocomposites nanoparticles potato selenium
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Rewriting Potato’s Evolutionary Destiny through Genome Design 认领 引用
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《Bulletin of the Chinese Academy of Sciences》 2026年第2期76-79,共4页
The 2026 Tan Kah Kee Science Award in Life Sciences goes to“Genome Design of Hybrid Potato”,a game-changing agricultural innovation achieved by a research team led by Prof.HUANG Sanwen from the Agricultural Genomics... The 2026 Tan Kah Kee Science Award in Life Sciences goes to“Genome Design of Hybrid Potato”,a game-changing agricultural innovation achieved by a research team led by Prof.HUANG Sanwen from the Agricultural Genomics Institute at Shenzhen(AGIS),Chinese Academy of Agricultural Sciences(CAAS).The outstanding work has transformed a clonally propagated crop into a sexually reproduced one for the first time in history. 展开更多
关键词 clonally propagated crop sexually reproduced potato clonally propagated genome design life sciences hybrid potato agricultural genomics institute
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Multi-omics analysis reveals dynamic metabolic and transcriptomic changes during potato tuber swelling 认领 引用
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作者 Jiangyue Long Wei Tan +2 位作者 Chunzhi Zhang Guangtao Zhu Zhong Zhang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第8期3282-3294,共13页
Potato is a vital global food source,yet the metabolic and transcriptional regulation governing tuber development and quality remains poorly understood.Here,we performed integrated metabolomic and transcriptomic analy... Potato is a vital global food source,yet the metabolic and transcriptional regulation governing tuber development and quality remains poorly understood.Here,we performed integrated metabolomic and transcriptomic analyses in wild and cultivated potato tubers,revealing dynamic and distinct metabolic accumulation patterns.The 849 metabolites exhibited 10 distinct temporal accumulation patterns,including six shared patterns between accessions and four genotype-specific patterns.The wild genotype exhibited an early decrease in lipids,followed by an increase in phenolic acids,whereas the cultivated genotype displayed an early increase in phenolic acids,accompanied by a decrease in some phenolic acids,amino acids,and flavonoids.A comparative analysis highlighted the cultivated genotype exhibited significantly lower levels of bitter steroidal glycoalkaloids(SGAs)but higher levels of beneficial flavonoids compared with its wild relative.Co-expression network analysis revealed 35 SGA-related and 57 phenylpropanoid-related genes that underlie metabolite dynamics.Notably,we functionally validated that the transcription factor StMYB113 plays a previously unknown role in positively regulating phenolic acid biosynthesis in tuber flesh.Our work provides a comprehensive map of tuber metabolism and a valuable resource for accelerating the genetic improvement of key potato quality traits. 展开更多
关键词 potato multi-omics steroidal glycoalkaloid phenylpropanoid transcriptional regulation
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Antibacterial Activity and Mechanism of Thyme Essential Oil Nanoemulsion Prepared with Potato Protein Modified by Transglutaminase (TGase) against Staphylococcus aureus and Escherichia coli: A Metabolomic Study 认领 引用
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作者 YUAN Ruoyun CHANG Tong +6 位作者 WANG Yilin XU Xin LÜFengzhang WANG Miao MA Chengye LI Hongjun WANG Chenjie 《食品科学》 EI CAS CSCD 北大核心 2026年第1期17-28,共12页
In this study,thyme essential oil(TEO)nanoemulsion(tPTNs)was constructed with transglutaminase(TGase)-modified potato protein,and its antibacterial activity and mechanism of action were evaluated and explored.Results ... In this study,thyme essential oil(TEO)nanoemulsion(tPTNs)was constructed with transglutaminase(TGase)-modified potato protein,and its antibacterial activity and mechanism of action were evaluated and explored.Results indicated that tPTNs exhibited great antibacterial activity against both Staphylococcus aureus and Escherichia coli,with minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of 2.5 and 5.0 mg/mL,respectively.Also,the antibacterial effects of tPTNs were concentration-dependent.We observed a significant decrease in the absolute value of the zeta potential,and significant increases in particle size,cell membrane hydrophobicity,conductivity,the release of metal ions,and the leakage of nucleic acid as the concentration of tPTNs increased from 0 mg/mL to MBC.Furthermore,sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE)demonstrated that protein synthesis was inhibited or even disrupted.Analysis by liquid chromatography-mass spectrometry(LC-MS)indicated that treatment with tPTNs caused significant changes in bacterial metabolites,1117 and 692 differential metabolites being found for S.aureus and E.coli,respectively.The differential metabolites were involved in nucleotide metabolism,amino acid metabolism,tricarboxylic acid cycle and other metabolic pathways.These findings provide valuable insights for the application of thyme essential oil as an efficient antibacterial agent and for the understanding of its mechanism of action. 展开更多
关键词 potato protein thyme essential oil nanoemulsions antibacterial mechanism metabolomics
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StCOMT1 enhances potato resistance to Fusarium sporotrichioides by regulating coumarin-related substance accumulation and the ROS system 认领 引用
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作者 Jiaqi Wang Biao Zhao +8 位作者 Dan Liu Fumeng He Chong Du Yunzhu Che Zengli Zhang Xu Feng Xue Wang Yingnan Wang Fenglan Li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第3期1020-1034,共15页
Caffeic acid-O-methyltransferase(COMT)is a crucial enzyme in the phenylpropanoid metabolic pathway,with significant roles in both the lignin and coumarin pathways.The function of COMT in plant disease resistance has b... Caffeic acid-O-methyltransferase(COMT)is a crucial enzyme in the phenylpropanoid metabolic pathway,with significant roles in both the lignin and coumarin pathways.The function of COMT in plant disease resistance has been demonstrated in several species.Our research identified the potato COMT gene family on a genome-wide scale and StCOMT1 as a candidate gene for enhancing potato disease resistance under DON induction through phylogenetic analyses combined with previously identified metabolic differences and weighted gene co-expression network analysis(WGCNA)results.In order to better understand the function of StCOMT1,heterologous expression and overexpression assays were conducted.StCOMT1 is localized in chloroplasts and was found to catalyze the methylation of substrates to produce ferulic acid and melatonin in vitro.Physiological parameters showed that,compared with wild-type potato plants,StCOMT1-overexpressing plants infected with Fusarium sporotrichioides exhibited smaller lesion areas and lower reactive oxygen species(ROS)levels.High-performance liquid chromatography(HPLC)and RT-qPCR analyses revealed organ-specific accumulation of coumarin-related compounds and organ-specific expression of their corresponding genes in StCOMT1-overexpressing plants post-inoculation.The results indicate that StCOMT1 overexpression in potatoes enhanced resistance to F.sporotrichioides by enhancing reactive oxygen species clearance and promoting organ-specific accumulation of coumarin-related compounds. 展开更多
关键词 potato COMT gene overexpression disease resistance ROS scavenging
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Functional roles of mashed potatoes in shaping dough rheology and bread storage stability 认领 引用
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作者 Siwen Zhai Jinzhe Han +4 位作者 Mengting Huang Xianfang Long Shuang Gao Xingchen Li Hongwei Cao 《Grain & Oil Science and Technology》 EI CAS 2026年第2期141-149,共9页
This study systematically investigated the influence of mashed potato incorporation on the rheological,microstructural,and quality attributes of wheat dough and bread.A comprehensive analysis was conducted,encompassin... This study systematically investigated the influence of mashed potato incorporation on the rheological,microstructural,and quality attributes of wheat dough and bread.A comprehensive analysis was conducted,encompassing dough rheology,texture profile,moisture distribution,and microstructure,alongside evaluations of bread specific volume,color,textural properties,sensory quality,and storage stability.The addition of mashed potato significantly altered dough properties,reducing hardness from 34.41 N to 22.26 N and decreasing viscoelasticity,primarily due to gluten dilution and modified water binding.Consequently,bread specific volume declined from 2.49 mL/g to 1.66 mL/g.However,enhanced Maillard reactions led to improved crust color brightness and overall sensory acceptability.Notably,incorporating 20% or more mashed potato effectively inhibited starch retrogradation during storage at 4℃.The findings indicate that a 20% addition level offers an optimal balance between quality improvement and structural integrity,whereas 30% represents the upper limit beyond which significant weakening of the bread structure occurs. 展开更多
关键词 Mashed potatoes Wheat bread Dough rheology Bread quality Starch retrogradation
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Effect of F01 Compound Inoculant on Potato Yield,Quality,and Soil Properties 认领 引用
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作者 WANG Xin-tong WEI Bao-yang 《Agricultural Science & Technology》 2026年第1期39-49,共11页
Pot and field experiments were conducted to systematically evaluate the effect of F01 compound inoculant on soil properties,potato yield,and potato quality.The results were summarized as follows:(1)F01 compound inocul... Pot and field experiments were conducted to systematically evaluate the effect of F01 compound inoculant on soil properties,potato yield,and potato quality.The results were summarized as follows:(1)F01 compound inoculant increased the content of available nutrients in the soil.Applying F01 compound inoculant at 60-90 kg/hm2significantly increased the content of available nitrogen,available phosphorus,and available potassium in the soil,and the application in the early and middle growth stages demonstrated particularly remarkable effect.However,excessive application inhibited the accumulation of soil nutrients.(2)F01 compound inoculant significantly improved the activities of urease,sucrase,catalase,and alkaline phosphatase in the soil.The medium dose treatment showed the best stability and comprehensive effect.(3)F01 compound inoculant increased the potato yield.Under field conditions,the F01 compound inoculant applied at 90 kg/hm2increased the potato yield by 35.59%compared with the control group.In addition,it significantly increased the weight and number of tubers per plant and the commercial potato rate.(4)F01 compound inoculant showed the potential of improving the quality of potato tubers.It exerted a positive effect on crude protein content,but had no significant effect on dry matter or starch content,which may be related to the genetic characteristics of the fresheating potato variety‘Jizhangshu 12’used.In summary,the F01 compound inoculant applied at 60-90 kg/hm2achieved synergistic effect of soil improvement and yield and quality enhancement,demonstrating application potential and promotion value.In the future,exploration should be conducted on its adaptability and stability under different soil types and potato varieties. 展开更多
关键词 F01 compound inoculant Potato Available nutrient Soil enzyme activity Yield Quality
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Genome-wide analysis of non-coding RNA reveals the role of a novel miR319c for tuber dormancy release process in potato 认领 引用 被引量：1
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作者 Shengyan Liu Jiangwei Yang +1 位作者 Ning Zhang Huaijun Si 《Horticulture Research》 SCIE CSCD 2025年第2期103-121,共19页
Tuber dormancy and sprouting are significant for potato cultivation,storage,and processing.Although the substantial role of microRNAs(miRNAs)in some biological processes has been recognized,the critical role of miRNA ... Tuber dormancy and sprouting are significant for potato cultivation,storage,and processing.Although the substantial role of microRNAs(miRNAs)in some biological processes has been recognized,the critical role of miRNA in breaking potato tuber dormancy is not well understood to date.In this investigation,we expand research on miRNA-mediated gene regulation in tuber dormancy release.In this work,204 known and 192 novel miRNAs were identified.One hundred thirty-six differentially expressed miRNAs(DE-miRNAs)were also screened out,of which 56 DE-miRNAs were regulated by temperature during tuber dormancy release.Additionally,degradome sequencing revealed that 821 target genes for 202 miRNAs were discovered.Among them,63 target genes and 48 miRNAs were predicted to be involved in plant hormone signaling pathways.This study used degradome sequencing,tobacco cotransformation system,and β-glucuronidase(GUS)staining technology to confirm that stu-miR319c can target StTCP26 and StTCP27 and effectively suppress their expression.The transgenic approach exhibited that stu-miR319c overexpressed tubers sprouted in advance,while silent expression of stu-miR319c showed delayed sprouting.Treatment of wild-type tubers with exogenous MeJA revealed that 1 mg/L MeJA significantly broke dormancy and enhanced potato sprouting ability.Furthermore,transgenic tubers revealed variance in jasmonic acid(JA)content and relative expression of genes associated with the JA synthesis pathway,including StAOC,StLOX2,and StLOX4,suggesting that the miR319c may participate in the JA pathway to regulate tuber dormancy release.In summary,our research offers evidence that miRNA regulates potato dormancy release and supports the idea that stu-miR319c is a unique epigenetic regulator for dormancy-sprouting transition in potatoes. 展开更多
关键词 potato cultivation genome wide analysis miRNA non coding RNA tuber dormancy breaking potato tuber dormancy biological processes sprouting
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Comparative analysis of two stored sweet potato varieties using integrated transcriptomics and metabolomics 认领 引用
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作者 Xinyi Feng Jinhua Zuo +9 位作者 Yunxiang Wang Chunmei Bai Shaoqing Chen Lili Ma Xuelian He Jiejie Tao Shiyu Liu Yiting Ren Demei Meng Yanyan Zheng 《Vegetable Research》 2025年第1期370-382,共13页
Texture,color,antioxidant capacity,and disease resistance of sweet potato varieties differ,but information on metabolic differences among varieties is limited.This research investigated the alterations in volatile sub... Texture,color,antioxidant capacity,and disease resistance of sweet potato varieties differ,but information on metabolic differences among varieties is limited.This research investigated the alterations in volatile substances,gene expression quantities,and metabolites during the storage process of two varieties of sweet potatoes:'Xiguahong'and'Yanshu25'.The GC-IMS findings indicated that compounds possessing appealing aromas,including 2-ethylpyrazine,γ-terpinene,and ethyl isobutyrate,were more concentrated in'Xiguahong'.The expression levels of phenylpropanoid biosynthesis genes(PAL,CCR),flavor-related genes(MLS,LOX6),color-related genes(PSY2,CRTISO),and resistance-related genes(PPO1,GSTL)were higher in'Xiguahong'.Phenolic acids,flavonoids,terpenes,lignans,and coumarins content increased more in'Xiguahong'than in'Yanshu25'.This study provides insights into the molecular mechanisms underlying post-harvest quality changes in different sweet potato varieties. 展开更多
关键词 sweet potato metabolomics stored sweet potatoes storage process ethyl isobutyratewere sweet potatoes xiguahongandyanshu transcriptomics volatile substances
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Establishment of a rapid detection method for potato virus S based on real-time fluorescence and visualization of LAMP 认领 引用
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作者 WANG Yue ZHANG Yongpeng MA Yongqiang 《植物病理学报》 CAS CSCD 北大核心 2025年第5期1150-1164,共15页
Potato virus S(PVS)is one of the main viruses infecting potatoes,and it is widely distributed in the potato growing areas in China.To manage this virus,it is particularly important to develop a rapid and sensitive fie... Potato virus S(PVS)is one of the main viruses infecting potatoes,and it is widely distributed in the potato growing areas in China.To manage this virus,it is particularly important to develop a rapid and sensitive field PVS loop mediated isothermal amplification(LAMP)visual detection method.In this study,we designed primers based on the conserved fragment of the PVS CP gene sequence as a template for real-time fluorescence LAMP.By selecting the best primers,optimizing the reaction conditions and the reaction system,a rapid visua-lization-based LAMP detection method for PVS was successfully established with verified specificity and sensitivity.This new,rapid visual LAMP detection method for PVS can specifically identify PVS and visualize it within 1 hour.The detection limits are up to 10.24 pg·μL-1(cDNA),which is 20 times more sensitive than that of real-time fluorescence PCR,and no other viruses were detected with the designed primers.The established method was used to analyze 50 samples from the field to verify the accuracy of the method,and the results were basically consistent with the corresponding real-time fluorescence PCR results,indicating that the LAMP detection method established in this study can rapidly detect PVS and provide effective technical support for potato virus disease management. 展开更多
关键词 virus detection potato virus disease loop mediated isothermal amplification potato virus S qPCR
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Fine-mapping and candidate gene analysis of tuber eye depth in potato 认领 引用 被引量：3
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作者 Guiyan Fan Shaoguang Duan +8 位作者 Yuting Yang Yanfeng Duan Yinqiao Jian Jun Hu Zhiyuan Liu Yang-dong Guo Liping Jin Jianfei Xu Guangcun Li 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第3期1248-1259,共12页
Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breedin... Eye depth is an important agronomic trait affecting tubers'appearance,quality,and processing suitability.Hence,cultivating varieties with uniform shapes and shallow eye depth are important goals for potato breeding.In this study,based on the primary mapping of the tuber eyedepth locus using a small primary-segregating population,a large secondary-segregating population with 2100 individuals was used to map the eye-depth locus further.A major quantitative trait locus for eye-depth on chromosome 10 was identified(designated qEyd10.1)using BSAseq and traditional QTL mapping methods.The qEyd10.1 could explain 55.0%of the eye depth phenotypic variation and was further narrowed to a 309.10 kb interval using recombinant analysis.To predict candidate genes,tissue sectioning and RNA-seq of the specific tuber tissues were performed.Genes encoding members of the peroxidase superfamily with likely roles in indole acetic acid regulation were considered the most promising candidates.These results will facilitate marker-assisted selection for the shallow-eye trait in potato breeding and provide a solid basis for eye-depth gene cloning and the analysis of tuber eye-depth regulatory mechanisms. 展开更多
关键词 BSA-seq Eye depth Peroxidase Potato tuber Quantitative trait loci
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stu-miR159a negatively regulates anthocyanin-specific MYB transcription factor to mediate drought stress tolerance in potato 认领 引用 被引量：3
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作者 Yurong Deng Jiangwei Yang +5 位作者 Jingjing Wei Shengyan Liu Liang Yang Xiaofeng Wang Ning Zhang Huaijun Si 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第5期1917-1929,共13页
MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,t... MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,the role of miRNAs in drought regulation remains unclear in potato.In this work,the function of stu-miR159a was investigated in responding to drought stress in potato.Upon examination,StGAMyb-like1 was identified as the target gene for stu-miR159a.Overexpression of stu-miR159a(stu-miR159a OE plants) increased sensitivity to drought,interference with stu-miR159a activity by target mimics(stu-miR159a ST plants) resulted in drought resistance.During drought treatment,the target gene StGAMyb-like1 showed increased activation in stu-miR159a ST plants compared to non-transgenic plants.In contrast,drought stress induced weaker activation of the target gene in stu-miR159a OE plants.In stu-miR159a ST plants,the expression of critical genes in the anthocyanin biosynthesis pathway(StF3'5'H,StF3'H and StCHS2)was increased by decreasing stu-miR159a activity and simultaneously increasing that of StGAMyb-like1.Meanwhile,with drought treatment,stu-miR159a ST plants exhibited higher anthocyanin accumulation than non-transgenic ones,indicating enhanced antioxidant capacity and improved drought tolerance.The above data support that stu-miR159a is a negative regulator of drought stress and provide new insights into the stu-miR159a-mediated regulation of the anthocyanin biosynthesis pathway in controlling drought tolerance in potato. 展开更多
关键词 Potato miR159-GAMYB pathway Drought Anthocyanin Antioxidant activity
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Genetic diversity of the self-incompatibility locus in diploid potato 认领 引用 被引量：1
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作者 Xinyu Jing Duoduo Qian +6 位作者 Xiuhan Jiang Pei Wang Huihui Bao Dianjue Li Yanhui Zhu Guangtao Zhu Chunzhi Zhang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2025年第4期1448-1460,共13页
Re-domestication of diploid potato(Solanum tuberosum)into a seed crop is an innovative breeding method to accelerate genetic improvement.Seed propagation would allow hybrid production and mix superior alleles.However,... Re-domestication of diploid potato(Solanum tuberosum)into a seed crop is an innovative breeding method to accelerate genetic improvement.Seed propagation would allow hybrid production and mix superior alleles.However,almost all diploid potatoes in nature are self-incompatible(SI).Gametophytic self-incompatible(GSI)is a widespread SI in Solanaceae and is controlled by the S locus that contains a ribonuclease(S-RNase)and multiple F-box(SLFs);however,the genetic diversity of the S locus in potato is unclear.This study identified 21 S-RNase alleles involved in SI from 194 diploid potato accessions by large-scale transcriptome sequencing.The levels of amino acid similarity among different S-RNase proteins varied from 31.3 to 95.8%.S2 allele is the most widespread in 194 diploid potatoes and is mainly distributed in the S.tuberosum Group Phureja.Based on genomic annotation and expression analysis,we identified 12 potential functional SI male-determinant genes,S-locus F-box(SLFs),encoding F-box proteins in the S2 locus on a genomic region of approximately 13 Mb.Comparative genomics analysis showed that eight SLF genes are relatively conserved among four homozygous S locus.The Ka and Ks analysis suggested that S-RNase and intra-haplotypic SLF genes have co-evolved.These findings help select suitable pollinators,combine more hybrid combinations,and fully use heterosis to accelerate diploid potato breeding. 展开更多
关键词 potato self-incompatibility S-RNase SLF
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Transcriptional profiling during infection of potato NLRs and Phytophthora infestans effectors using cDNA enrichment sequencing 认领 引用 被引量：1
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作者 Amanpreet Kaur Vikrant Singh +6 位作者 Stephen Byrne Miles Armstrong Thomas M.Adams Brian Harrower Eleanor Gilroy Ewen Mullins Ingo Hein 《The Crop Journal》 SCIE CAS CSCD 2025年第1期31-40,共10页
An accurate assessment of host and pathogen gene expression during infection is critical for understanding the molecular aspects of host-pathogen interactions.Often,pathogen-derived transcripts are difficult to ascert... An accurate assessment of host and pathogen gene expression during infection is critical for understanding the molecular aspects of host-pathogen interactions.Often,pathogen-derived transcripts are difficult to ascertain at early infection stages owing to the unfavourable transcript representation compared to the host genes.In this study,we compare two sequencing techniques,RNAseq and enrichment sequencing(RenSeq and PenSeq)of cDNA,to investigate gene expression patterns in the doubled monoploid potato(DM)infected with the late blight pathogen Phytophthora infestans.Our results reveal distinct advantages of cDNA RenSeq and PenSeq over traditional RNAseq in terms of target gene representation and transcriptional quantification at early infection stages.Throughout the infection time course,cDNA enrichment sequencing enables transcriptomic analyses for more targeted host and pathogen genes.For highly expressed genes that were sampled in parallel by both cDNA enrichment and RNAseq,a high level of concordance in expression profiles is observed,indicative of at least semi-quantitative gene expression representation following enrichment. 展开更多
关键词 RxLR effector NLRs Late blight Potato cDNA sequencing RenSeq PenSeq RNAseq
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Comparative transcriptomic analyses of ethylene and 1-MCP controlling sprouting in Kokei No.14 organic sweet potatoes during storage 认领 引用
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作者 Xueqian Zang Guangwei Wu +4 位作者 Mingkai Peng Bei Wang Yanli Chen Jingjing Kou Guopeng Zhu 《Tropical Plants》 CSCD 2025年第1期236-250,共15页
The organic sweet potato cultivar Kokei No.14 is recognized for its high quality,nutritional benefits,and favorable growth performance in Hainan province(China).However,postharvest sprouting of Kokei No.14 presents a ... The organic sweet potato cultivar Kokei No.14 is recognized for its high quality,nutritional benefits,and favorable growth performance in Hainan province(China).However,postharvest sprouting of Kokei No.14 presents a significant challenge during storage.To clarify sprouting mechanism,we performed several treatments including continuous air(Air),1-MCP(30μL·L-1),ethylene(200μL·L-1),and combined ethylene and 1-MCP.The sprouting rates,quality changes,and transcriptomic analysis after treatment were investigated.The results indicated that ethylene,1-MCP,and their combined treatment significantly inhibited germination and respiration rates,reduced weight loss,and extended the root shelf life.The combination of ethylene and 1-MCP was more effective in reducing sprouting than the individual application of either compound.Meanwhile,ethylene treatment enhanced the color vibrancy and flavor of the roots.Transcriptome analysis identified 2,069 transcription factors,predominantly AP2/ERF,MYB,bHLH,and WRKY,which were crucial in regulating root sprouting and quality changes during germination.A total of 360 differentially expressed genes(DEGs)were assigned to 19 KEGG pathways including starch and sucrose metabolism,plant hormone signal transduction,and the flavonoid pathway by comparative transcriptome.Weighted Gene Co-Expression Network Analysis(WGCNA)indicated that these DEGs were primarily associated with physiological indicators such as sprout number,sprout length,weight loss,respiration rate,color,starch,and sucrose content.DEGs in these pathways played a crucial role in regulating sweet potato sprouting after ethylene and 1-MCP treatment.This research provided a theoretical basis and practical guidance in germination inhibition and quality improvement of organic Kokei No.14 sweet potato roots. 展开更多
关键词 postharvest storage Kokei no transcriptomic analysis organic sweet potatoes ethylene mcp organic sweet potato sprouting
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Mechanisms of ozone regulation of sweet potato storage quality 认领 引用
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作者 Yuelan Liu Yubin Wang +8 位作者 Shiyu Liu Jinhua Zuo Zhaoze Sang Xuelian He Shaoqing Chen Xinyuan Zhou Zhe Gao Yanyan Zheng Yunxiang Wang 《Vegetable Research》 2025年第1期272-285,共14页
Ozone,as a safe and environmentally friendly sterilization method,has been applied in the field of fruit and vegetable preservation.However,its preservation effects and underlying mechanisms on postharvest sweet potat... Ozone,as a safe and environmentally friendly sterilization method,has been applied in the field of fruit and vegetable preservation.However,its preservation effects and underlying mechanisms on postharvest sweet potatoes remain unclear.This study revealed the mechanism of ozone treatment in preserving sweet potatoes through transcriptomics and metabolomics analysis.After ozone treatment,the relative content of lipids and phenolic acid metabolites in sweet potatoes increased,while the relative content of amino acids decreased.In addition,ozone treatment upregulated the expression of genes related to carotenoid biosynthesis(PSY,D27,ZEP,Z-ISO),sucrose metabolism(SPS,SUS,INV,S-AI),and disease resistance(RPM1,RPP13-LK4,R1-A,TLP).Conversely,it downregulated the expression of genes involved in lignin biosynthesis(PAL,CCoAOMT,CCR1,C4H,4CL,CAD,CA4H),and ethylene biosynthesis and signal transduction(ETR2,CTR1,MATs3,ACO1,ACO3,NTF4,ACC,ERFC3,ERF4).These changes helped maintain the color,sweetness,and texture of sweet potatoes,effectively delaying their senescence. 展开更多
关键词 sweet potato sweet potatoes metabolomics phenolic acid metabolites metabolomics analysisafter transcriptomics fruit vegetable sterilization methodhas
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