Diabetic retinopathy,a prevalent and vision-threatening microvascular complication of diabetes mellitus,is the leading cause of blindness among middle-aged and elderly individuals.Natural diterpenoids isolated from Si...Diabetic retinopathy,a prevalent and vision-threatening microvascular complication of diabetes mellitus,is the leading cause of blindness among middle-aged and elderly individuals.Natural diterpenoids isolated from Siegesbeckia pubescens demonstrate potent anti-inflammatory properties.This study aimed to identify novel bioactive diterpenoids from S.pubescens and investigate their effects on oxidative stress and inflammatory responses in diabetic retinopathy,both in vitro and in vivo.Three new ent-pimarane-type diterpenoids(1–3)and six known compounds(4–9)were isolated from the aerial parts of S.pubescens.Their structures were elucidated through spectroscopic data interpretation,and absolute configurations were determined by comparing calculated and experimental electronic circular dichroism(ECD)spectra.Among these compounds,14β,16-epoxy-ent-3β,15α,19-trihydroxypimar-7-ene(5)exhibited the most potent protective effect against high glucose and interleukin-1β(IL-1β)-stimulated human retinal endothelial cells.Mechanistically,compound 5 promoted endothelial cell survival while ameliorating oxidative stress and inflammatory response in diabetic retinopathy,both in vivo and in vitro.These findings not only suggest that diterpenoids such as compound 5 are important anti-inflammatory constituents in S.pubescens,but also indicate that compound 5 may serve as a lead compound for preventing or treating vascular complications associated with diabetic retinopathy.展开更多
The flower of Syringa pubescens Turcz.(SP),was used as both medicine and food in China,exhibited various biological activities.However,it remains unknown whether SP affects ameliorative nonalcoholic steatohepatitis(NA...The flower of Syringa pubescens Turcz.(SP),was used as both medicine and food in China,exhibited various biological activities.However,it remains unknown whether SP affects ameliorative nonalcoholic steatohepatitis(NASH).In this study,the improvement of SP ethyl acetate extract(SPE)on NASH was evaluated and the potential mechanisms were explored.The glycosides of SPE were determined by HPLC method.Hep G2 cell lines were treated with free fatty acid to clarify the improvement effect and mechanisms of SPE on NASH in vitro.C57BL/6J mice were treated by 60%kcal high-fat diet(HFD)combined with carbon tetrachloride(CCl_4)to establish the NASH model in vivo.The results showed SPE could inhibit the hepatic injury and lipid steatosis by decreasing the levels of alanine aminotransferase,aspartate aminotransferase,triglyceride and total cholesterol in vitro and in vivo.The SPE suppressed the oxidative stress and inflammation by restraining the generation of reactive oxygen species,malondialdehyde,interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α)and enhancing the activity of antioxidant enzymes including superoxide dismutase,catalase and glutathione peroxidase in NASH model.Moreover,SPE declined the level of fibrosis markers including hydroxyproline,type I collagen andα-smooth muscle actin(α-SMA)in HFD combined CCl_4-induced mice.Mechanically,SPE inhibited hepatocellular Kelch like ECH associated protein 1 expression and promoted nuclear erythroid 2-related factor 2(Nrf2)nuclear translocation,which suppressed the phosphorylation of IκBα/NF-κB.In addition,SPE down-regulated the level of transforming growth factorβ1(TGF-β1)and phosphorylation of Smad2 to mitigate fibrosis.In brief,SPE could significantly alleviate lipid accumulation,oxidative stress,inflammation and fibrosis via regulating Nrf2/HO-1,IκBα/NF-κB and TGF-β1/Smad2 pathways in the progression of NASH,which indicated that SPE had the potential to be a novel and effective drug or food supplements for the improvement of NASH.展开更多
Ground cherry(Physalis pubescens L.) is a kind of berry fruit favored by consumers in China; however, this fruit is particularly vulnerable to physiological senescence and pathogen attack during the traditional cold s...Ground cherry(Physalis pubescens L.) is a kind of berry fruit favored by consumers in China; however, this fruit is particularly vulnerable to physiological senescence and pathogen attack during the traditional cold storage period. In order to maintain storage quality, a 1.5%(w/w) chitosan(CS) water solution containing 50 mg/L of natamycin(NA) was introduced. After all treatments were completed, the fruit was stored at 0°C and sampled every 10 d. At each sampling date, the following tests were performed: mold and yeast analyses; enzyme activity and content analyses which included superoxide dismutase(SOD), ascorbate peroxidase(APX), and malondialdehyde(MDA); and color analysis. In addition, a sensory evaluation was carried out for quality assessment at the end of the storage period. The results showed that the application of a chitosan coating combined with natamycin(CSNA) enhanced the activity of superoxide dismutase(SOD) and ascorbate peroxidase(APX), reduced the physiological rate of senescence, and inhibited pathogen attack. Thus, CSNA treatment can maintain ground cherries at an acceptable level of storage quality for 50 d.展开更多
Five new diterpenoids isolated from Siegesbeckia pubescens, pubesides A similar toE, were established as ent-2 alpha ,15,16-trihydroxypimar-8(14)-en-2-O-beta -D-glucopyranoside (1), ent-15,16,19-trihydroxypimar-8(14)-...Five new diterpenoids isolated from Siegesbeckia pubescens, pubesides A similar toE, were established as ent-2 alpha ,15,16-trihydroxypimar-8(14)-en-2-O-beta -D-glucopyranoside (1), ent-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-glucopyranoside (2), beta -D-glucopyranosyl-ent-15,16-dihydroxypimar-8(14)-en-19-oiclate (3), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-gluco-pyranoside (4), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-glucopyranoside-15,16-acetonide (5) by 1D and 2D NMR techniques.展开更多
A new triterpene saponin with 28-nor-urs-12(13),18(17)-dien-3β-ol as aglycone,named ilexsaponin C(1)was isolated from the roots of Ilex pubescens,together with three known saponins 2,3 and 4.The structure of 1 was el...A new triterpene saponin with 28-nor-urs-12(13),18(17)-dien-3β-ol as aglycone,named ilexsaponin C(1)was isolated from the roots of Ilex pubescens,together with three known saponins 2,3 and 4.The structure of 1 was elucidated on the basis of spectral analysis including 1D and 2D NMR and HR-ESI-MS.Saponins 1 and 4 exhibited significant XOD inhibitory activity in the test.展开更多
One new compound,ilexlignan A(1),was isolated from the root of llex pubescens.Its structure was elucidated by the combination of one-and two-dimensional NMR analysis,mass spectrometry,CD spectrum measurement and chemi...One new compound,ilexlignan A(1),was isolated from the root of llex pubescens.Its structure was elucidated by the combination of one-and two-dimensional NMR analysis,mass spectrometry,CD spectrum measurement and chemical evidence.展开更多
The height growth of bamboo in 2008 and 2009 as well as the water contents of bamboo stump,leaves,straw,and rhizome in 2009 were measured at the Tianmu Mountain Nature Reserve.The results revealed that the relationshi...The height growth of bamboo in 2008 and 2009 as well as the water contents of bamboo stump,leaves,straw,and rhizome in 2009 were measured at the Tianmu Mountain Nature Reserve.The results revealed that the relationship between the height growth(Pn)and growth time(t)showed S-shaped curve in the two years;the water contents of bamboo stump,leaf,straw,rhizome and bamboo shoot were 0.50-2.31,0.46-1.8,0.50-1.38,0.56-1.58 and 0.89-8.96,respectively.Among them,the water content of bamboo shoot was the highest and noticeable changed,followed by bamboo stump,leaves,rhizome and straw in order.The water contents of the underground and aboveground parts respectively were 0.34-0.62 and 0.33-0.57.展开更多
One new ent-pimarane diterpenoid ent-16-nor-3-oxo-pimar-8(14)-en-15-al (1) together with four known diterpenoids kirenol (2), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-ene (3), darutigenol (4) and darutosi...One new ent-pimarane diterpenoid ent-16-nor-3-oxo-pimar-8(14)-en-15-al (1) together with four known diterpenoids kirenol (2), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-ene (3), darutigenol (4) and darutoside (5) were isolated from the ethanol extract of Siegesbeckia pubescens. The planar structures and relative configurations of these compounds were elucidated by comprehensive spectroscopic analysis.展开更多
[Objective] The research aimed to study the response and accumulation characteristics of Cd in Polygonum pubescens Bl.[Method] Taking Hoagland nutritional solution as the culture substrate,eight kinds of Cd concentrat...[Objective] The research aimed to study the response and accumulation characteristics of Cd in Polygonum pubescens Bl.[Method] Taking Hoagland nutritional solution as the culture substrate,eight kinds of Cd concentrations (0,25,50,100,200,500,800,1 000 mg/L) were set up.[Result] Under low concentrations of Cd (25 and 50 mg/L),the biomass of P.pubescens had no significant difference with CK (P0.05).Under all the test concentrations of Cd,Cd content in the roots and shoots of P.pubescens exceeded 100 mg/kg and the enrichment coefficients of Cd were over 1.With the increasing of Cd concentration,Cd content and Cd accumulation amount in P.pubescens increased significantly.When Cd concentration exceeded 50 mg/L,translocation capacity coefficient was greater than 1.[Conclusion] Polygonum pubescens has high tolerance and accumulation capacity of Cd,and it may has some potential on the phytoremediation of Cd-contaminated soil.展开更多
Ultrastructural changes in secondary wall formation of Phyllostachys pubescens Mazel fiber were investigated with transmission electron microscopy. Fiber developed initially with the elongation of cells containing rib...Ultrastructural changes in secondary wall formation of Phyllostachys pubescens Mazel fiber were investigated with transmission electron microscopy. Fiber developed initially with the elongation of cells containing ribosomes, mitochondria and Golgi bodies in the dense cytoplasm. During the wall thickening, the number of rough endoplasmic reticulum and Golgi bodies increased apparently. There were two kinds of Golgi vesicles, together with the ones from endoplasmic reticulum formed transport vesicles. Many microtubules were arranged parallel to the long axis of the cell adjacent to the plasmalemma. Along with the further development of fiber, polylamellate structure of the secondary wall appeared, with concurrent agglutination of chromatin in the nucleus, swelling and disintegration of organelles, while cortical microtubules were still arranged neatly against the inner side of plasmalemma. Lomasomes could be observed between the wall and plasmalemma. The results indicated that the organelles, such as Golgi bodies together with small vesicles, rough endoplasmic reticulum and lomasomes, played the key role in the thickening and lignification of the secondary wall of bamboo fiber, though cortical microtubules were correlative with the process as well.展开更多
Siegesbeckia pubescens(SP) has been used as a traditional medicine for the treatment of and inflammatory diseases. However, the activities of SP against hepatocellular carcinoma and the related mechanisms remain unc...Siegesbeckia pubescens(SP) has been used as a traditional medicine for the treatment of and inflammatory diseases. However, the activities of SP against hepatocellular carcinoma and the related mechanisms remain unclear. The present study aimed to examine the effects of the essential oil of SP(SPEO) on the proliferation of hepatocellular carcinoma cells and the possible mechanisms. The growth inhibition of Hep G2 cells was analyzed by MTT assay. Hoechst 33258 and fluorescence microscopy were utilized to observe the nuclear morphological changes of apoptotic cells. Flow cytometry was used to detect cell apoptosis and cell cycle. The expressions of the target proteins were detected by Western blotting. The results showed that SPEO obviously inhibited the proliferation of Hep G2 cells in a dose-dependent manner. SPEO activated a series of apoptotic proteins in Hep G2 cells, increasing expression levels of Bax, caspase-3 and caspase-9, and decreasing the bcl-2 expression level. SPEO displayed promising anti-hepatocellular carcinoma activities in vitro, partly by inducing apoptosis in Hep G2 cells through activating the mitochondrial pathway.展开更多
We investigated the culm form for one of the largest bamboo species, Phyllostachys pubescens Mazel ex Houz. in relation to the me- chanical constraint principles, i.e., elastic, stress and geometric similarity. The fi...We investigated the culm form for one of the largest bamboo species, Phyllostachys pubescens Mazel ex Houz. in relation to the me- chanical constraint principles, i.e., elastic, stress and geometric similarity. The fine-resolution analysis of the culm taper indicated that the culm for P. pubescens consisted of three or four segments with various forms, except for the butt swell. This implied that the taper of the whole culm for P. pubescens could be expressed by neither of these principles. The regression slope between culm height and diameter at breast height on the double logarithmic coordinates was 0.629, which was significantly different from the values predicted from these principles. In conclusion, none of these mechanical constraint principles can express the culm taper and height-diameter relationship, and there may be a need for a more complicated model to express the culm form for P. pubescens.展开更多
[Objective] This study aimed to understand the species and behaviors of flower-visiting insects on Mussaenda pubescens Ait.f.in the Mount Emei National Nature Reserve.[Method] The species,visiting frequency,visiting t...[Objective] This study aimed to understand the species and behaviors of flower-visiting insects on Mussaenda pubescens Ait.f.in the Mount Emei National Nature Reserve.[Method] The species,visiting frequency,visiting time and behaviors of flower-visiting insects on M.pubescens were studied by collection,identification and image capture.[Result] The flower-visiting insects on M.pubescens in the Mount Emei covered total 26 species with 21 species from Lepidoptera,2 species from Hymenoptera,and 3 species from Diptera.Thus,Lepidoptera were the main group of pollinators for c in the Mount Emei National Nature Reserve.The daily activity rhythms of Lepidoptera,Hymenoptera and Diptera showed different characteristics,and they were characterized by single-peak type and double-peak type.[Conclusion] This study will provide certain theoretical basis for studies on the diversity of flower-visiting insects on plants in the Mount Emei National Nature Reserve.展开更多
A laboratory reactor was designed to test the effect of the interference instant of HY on the pyrolysis of pubescens.The time instant for intermediate species from pyrolysis to contact HY was controlled by varying the...A laboratory reactor was designed to test the effect of the interference instant of HY on the pyrolysis of pubescens.The time instant for intermediate species from pyrolysis to contact HY was controlled by varying the position of the catalyst bed relative to the pyrolytic cell.It was found that the effect of the interference instant was significant on the variation of different intermediate species,and the yield and quality of the products.The results also showed that,with the increase in the distance between the pyrolytic cell and the catalyst bed,the yield of liquid and relative content of the organics such as aldehyde,phenols,etc.,decreased,while the yield of residue and relative content of acetic acid increased.The deoxygenation of the intermediate species was favored when the catalyst exerted its performance on them immediately after their formation.展开更多
A new angelol-type coumarin glucoside,6-[(1R,2R)-1,2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin,was isolated from the roots of Angelica pubescens.Its structure was elucidated on the basis of spectra...A new angelol-type coumarin glucoside,6-[(1R,2R)-1,2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin,was isolated from the roots of Angelica pubescens.Its structure was elucidated on the basis of spectral analysis.展开更多
Development of diabetic cataract is mainly associated with the accumulation of sorbitol via the polyol pathway through the action of Aldose reductase(AR).Hence,AR inhibitors are considered as potential agents in the m...Development of diabetic cataract is mainly associated with the accumulation of sorbitol via the polyol pathway through the action of Aldose reductase(AR).Hence,AR inhibitors are considered as potential agents in the management of diabetic cataract.This study explored the AR inhibition potential of Hemidesmus indicus var.pubescens root extract by in silico and ex vivo methods.Molecular docking studies(Auto Dock tool)betweenβ-sitosterol,hemidesminine,hemidesmin-1,hemidesmin-2,and AR showed thatβ-sitosterol(−10.2 kcal/mol)and hemidesmin-2(−8.07 kcal/mol)had the strongest affinity to AR enzyme.Ex vivo studies were performed by incubating isolated goat lenses in artificial aqueous humor using galactose(55 mM)as cataract inducing agent at room temperature(pH 7.8)for 72 h.After treatment with Vitamin E acetate−100μg/mL(standard)and test extract(500 and 1000μg/mL)separately,the estimation of biochemical markers showed inhibition of lens AR activity and decreased sorbitol levels.Additionally,extract also normalized the levels of antioxidant markers like SOD,CAT,GSH.Our results showed evidence that H.indicus var.pubescens root was able to prevent cataract by prevention of opacification and formation of polyols that underlines its potential as a possible therapeutic agent against diabetic complications.展开更多
Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods:The ind...Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods:The individual compounds were isolated using column chromatography and were characterized by spectroscopic techniques.The antioxidant activity was evaluated for all individual isolated compounds by DPPH method using L-Ascorbic acid as standard and cytotoxicity was assessed for the extract and the hyoscyamine by MTT assay, caspase test and RT-PCR study.Results:The antioxidant activity of the isolated compounds and the extract increased as the concentration increased.One of the isolated compound hyoscyamine showed the high antioxidant activity.The extract and the hyoscyamine dose-dependently decreased the cell viability in HepG2 cells.Hyoscyamine induced caspase-3 mediated apoptosis. Up regulation of p53 gene expression provides cue for apoptotic activity of hyoscyamine. Conclusions:The results indicate that hexane extract possessed potent antioxidant and cytotoxic activity and hyoscyamine is the principal bioactive compound in hexane extract.展开更多
Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size ...Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size but with great development potential. Plus selection on phenotype of Toona ciliata and Toona ciliata var.pubescens, molecular genetic marker of Toona ciliata var. pubescens, research progress in physiology and ecology of Toona ciliata and Toona ciliata var.pubescens were summarized in this paper. Suggestions and prospect forecast were proposed for relative research and utilization.展开更多
To accurately calculate carbon fixation capacity of P.pubescens forest in Hunan Province and promote the balanced and stable development of P.pubescens forestry in Hunan Province,standard sample plot was set in major ...To accurately calculate carbon fixation capacity of P.pubescens forest in Hunan Province and promote the balanced and stable development of P.pubescens forestry in Hunan Province,standard sample plot was set in major producing area of P.pubescens in Hunan Province,and biomass of 258 P.pubescens was measured according to DBH and age class.Multivariate regression method was used to establish univariate (DBH as independent variable) and bivariate (DBH and age class as independent variables) models of total aboveground biomass and organ biomass of P.pubescens .Model evaluation and test showed that each model accorded with the applicable precision and had the appropriate prediction level.展开更多
Toona ciliata and Toona ciliata var. pubescens are rare timber species. In China, Toona ciliata and Toona ciliata var. pubescens distribute sporadical y with smal popuIation size but have huge deveIopment potential. T...Toona ciliata and Toona ciliata var. pubescens are rare timber species. In China, Toona ciliata and Toona ciliata var. pubescens distribute sporadical y with smal popuIation size but have huge deveIopment potential. The paper reviewed the research progress of their seedIing breeding, nutrient characteristics, fertiIization technoIogy, afforestation design, timber utiIization, chemistry and pharmacoIogy, and then Iooked into the future research and utiIization of Toona ciliata and Toona ciliata var. pubescens.展开更多
基金supported by the National Natural Science Foundation of China (No.82073728)the Natural Science Foundation of Shandong Province (No.ZR2021MH357)。
摘要Diabetic retinopathy,a prevalent and vision-threatening microvascular complication of diabetes mellitus,is the leading cause of blindness among middle-aged and elderly individuals.Natural diterpenoids isolated from Siegesbeckia pubescens demonstrate potent anti-inflammatory properties.This study aimed to identify novel bioactive diterpenoids from S.pubescens and investigate their effects on oxidative stress and inflammatory responses in diabetic retinopathy,both in vitro and in vivo.Three new ent-pimarane-type diterpenoids(1–3)and six known compounds(4–9)were isolated from the aerial parts of S.pubescens.Their structures were elucidated through spectroscopic data interpretation,and absolute configurations were determined by comparing calculated and experimental electronic circular dichroism(ECD)spectra.Among these compounds,14β,16-epoxy-ent-3β,15α,19-trihydroxypimar-7-ene(5)exhibited the most potent protective effect against high glucose and interleukin-1β(IL-1β)-stimulated human retinal endothelial cells.Mechanistically,compound 5 promoted endothelial cell survival while ameliorating oxidative stress and inflammatory response in diabetic retinopathy,both in vivo and in vitro.These findings not only suggest that diterpenoids such as compound 5 are important anti-inflammatory constituents in S.pubescens,but also indicate that compound 5 may serve as a lead compound for preventing or treating vascular complications associated with diabetic retinopathy.
基金supported by grants from the National Natural Science Foundation of China(U1804175,32270418)the Joint Fund of Provincial Science and Technology Research and Development plan of Henan Province(232103810055,232301420100)。
摘要The flower of Syringa pubescens Turcz.(SP),was used as both medicine and food in China,exhibited various biological activities.However,it remains unknown whether SP affects ameliorative nonalcoholic steatohepatitis(NASH).In this study,the improvement of SP ethyl acetate extract(SPE)on NASH was evaluated and the potential mechanisms were explored.The glycosides of SPE were determined by HPLC method.Hep G2 cell lines were treated with free fatty acid to clarify the improvement effect and mechanisms of SPE on NASH in vitro.C57BL/6J mice were treated by 60%kcal high-fat diet(HFD)combined with carbon tetrachloride(CCl_4)to establish the NASH model in vivo.The results showed SPE could inhibit the hepatic injury and lipid steatosis by decreasing the levels of alanine aminotransferase,aspartate aminotransferase,triglyceride and total cholesterol in vitro and in vivo.The SPE suppressed the oxidative stress and inflammation by restraining the generation of reactive oxygen species,malondialdehyde,interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α)and enhancing the activity of antioxidant enzymes including superoxide dismutase,catalase and glutathione peroxidase in NASH model.Moreover,SPE declined the level of fibrosis markers including hydroxyproline,type I collagen andα-smooth muscle actin(α-SMA)in HFD combined CCl_4-induced mice.Mechanically,SPE inhibited hepatocellular Kelch like ECH associated protein 1 expression and promoted nuclear erythroid 2-related factor 2(Nrf2)nuclear translocation,which suppressed the phosphorylation of IκBα/NF-κB.In addition,SPE down-regulated the level of transforming growth factorβ1(TGF-β1)and phosphorylation of Smad2 to mitigate fibrosis.In brief,SPE could significantly alleviate lipid accumulation,oxidative stress,inflammation and fibrosis via regulating Nrf2/HO-1,IκBα/NF-κB and TGF-β1/Smad2 pathways in the progression of NASH,which indicated that SPE had the potential to be a novel and effective drug or food supplements for the improvement of NASH.
基金supported by the National High-Tech R&D Program(863)of China(No.2012AA101703)
摘要Ground cherry(Physalis pubescens L.) is a kind of berry fruit favored by consumers in China; however, this fruit is particularly vulnerable to physiological senescence and pathogen attack during the traditional cold storage period. In order to maintain storage quality, a 1.5%(w/w) chitosan(CS) water solution containing 50 mg/L of natamycin(NA) was introduced. After all treatments were completed, the fruit was stored at 0°C and sampled every 10 d. At each sampling date, the following tests were performed: mold and yeast analyses; enzyme activity and content analyses which included superoxide dismutase(SOD), ascorbate peroxidase(APX), and malondialdehyde(MDA); and color analysis. In addition, a sensory evaluation was carried out for quality assessment at the end of the storage period. The results showed that the application of a chitosan coating combined with natamycin(CSNA) enhanced the activity of superoxide dismutase(SOD) and ascorbate peroxidase(APX), reduced the physiological rate of senescence, and inhibited pathogen attack. Thus, CSNA treatment can maintain ground cherries at an acceptable level of storage quality for 50 d.
基金Projec twas supported by the Applied Basic Research Foundation of Yunnan Province (97C089M).
摘要Five new diterpenoids isolated from Siegesbeckia pubescens, pubesides A similar toE, were established as ent-2 alpha ,15,16-trihydroxypimar-8(14)-en-2-O-beta -D-glucopyranoside (1), ent-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-glucopyranoside (2), beta -D-glucopyranosyl-ent-15,16-dihydroxypimar-8(14)-en-19-oiclate (3), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-gluco-pyranoside (4), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-en-19-O-beta -D-glucopyranoside-15,16-acetonide (5) by 1D and 2D NMR techniques.
基金financially supported by the Postgraduate Innovation Project of Jiangsu Province,China(No.CX09B_284Z)
摘要A new triterpene saponin with 28-nor-urs-12(13),18(17)-dien-3β-ol as aglycone,named ilexsaponin C(1)was isolated from the roots of Ilex pubescens,together with three known saponins 2,3 and 4.The structure of 1 was elucidated on the basis of spectral analysis including 1D and 2D NMR and HR-ESI-MS.Saponins 1 and 4 exhibited significant XOD inhibitory activity in the test.
基金Natural Science Foundation of Guangdong Province of China(No.7017651)Program for New Century Excellent Talents in University of Peoples Republic of China(No.NCET-04-0289).
摘要One new compound,ilexlignan A(1),was isolated from the root of llex pubescens.Its structure was elucidated by the combination of one-and two-dimensional NMR analysis,mass spectrometry,CD spectrum measurement and chemical evidence.
基金Supported by Science and Technology Department 973 Program(2005CB422207,2002CB111504,2002CB410811,2005CB422208)Science and Technology Department Data Share Platform Construction Program(2006DKA32300-08)+2 种基金Science Department National Cooperation Program(200073819)National Natural Science Fund Program(40671132)Scientific Fundamental Work Special Program(2007FY110300-8)~~
摘要The height growth of bamboo in 2008 and 2009 as well as the water contents of bamboo stump,leaves,straw,and rhizome in 2009 were measured at the Tianmu Mountain Nature Reserve.The results revealed that the relationship between the height growth(Pn)and growth time(t)showed S-shaped curve in the two years;the water contents of bamboo stump,leaf,straw,rhizome and bamboo shoot were 0.50-2.31,0.46-1.8,0.50-1.38,0.56-1.58 and 0.89-8.96,respectively.Among them,the water content of bamboo shoot was the highest and noticeable changed,followed by bamboo stump,leaves,rhizome and straw in order.The water contents of the underground and aboveground parts respectively were 0.34-0.62 and 0.33-0.57.
基金supported by the National Natural Science Foundation of China(Grant No.30973628)the National Science and Technology Project of China(Grant No.2012ZX09103201-022)
摘要One new ent-pimarane diterpenoid ent-16-nor-3-oxo-pimar-8(14)-en-15-al (1) together with four known diterpenoids kirenol (2), ent-2-oxo-15,16,19-trihydroxypimar-8(14)-ene (3), darutigenol (4) and darutoside (5) were isolated from the ethanol extract of Siegesbeckia pubescens. The planar structures and relative configurations of these compounds were elucidated by comprehensive spectroscopic analysis.
基金Supported by National Science Foundation of China(30560032)Guangxi Key Laboratory Foundation (0702k023)~~
摘要[Objective] The research aimed to study the response and accumulation characteristics of Cd in Polygonum pubescens Bl.[Method] Taking Hoagland nutritional solution as the culture substrate,eight kinds of Cd concentrations (0,25,50,100,200,500,800,1 000 mg/L) were set up.[Result] Under low concentrations of Cd (25 and 50 mg/L),the biomass of P.pubescens had no significant difference with CK (P0.05).Under all the test concentrations of Cd,Cd content in the roots and shoots of P.pubescens exceeded 100 mg/kg and the enrichment coefficients of Cd were over 1.With the increasing of Cd concentration,Cd content and Cd accumulation amount in P.pubescens increased significantly.When Cd concentration exceeded 50 mg/L,translocation capacity coefficient was greater than 1.[Conclusion] Polygonum pubescens has high tolerance and accumulation capacity of Cd,and it may has some potential on the phytoremediation of Cd-contaminated soil.
摘要Ultrastructural changes in secondary wall formation of Phyllostachys pubescens Mazel fiber were investigated with transmission electron microscopy. Fiber developed initially with the elongation of cells containing ribosomes, mitochondria and Golgi bodies in the dense cytoplasm. During the wall thickening, the number of rough endoplasmic reticulum and Golgi bodies increased apparently. There were two kinds of Golgi vesicles, together with the ones from endoplasmic reticulum formed transport vesicles. Many microtubules were arranged parallel to the long axis of the cell adjacent to the plasmalemma. Along with the further development of fiber, polylamellate structure of the secondary wall appeared, with concurrent agglutination of chromatin in the nucleus, swelling and disintegration of organelles, while cortical microtubules were still arranged neatly against the inner side of plasmalemma. Lomasomes could be observed between the wall and plasmalemma. The results indicated that the organelles, such as Golgi bodies together with small vesicles, rough endoplasmic reticulum and lomasomes, played the key role in the thickening and lignification of the secondary wall of bamboo fiber, though cortical microtubules were correlative with the process as well.
基金financially supported by grants from the Major Research Project of Education Department of Hubei Province of China(No.D20131103)National Natural Science Foundation of China(No.81573561)the Introduction Project of Hangzhou Medical College,Zhejiang Province,China(No.2015B08)
摘要Siegesbeckia pubescens(SP) has been used as a traditional medicine for the treatment of and inflammatory diseases. However, the activities of SP against hepatocellular carcinoma and the related mechanisms remain unclear. The present study aimed to examine the effects of the essential oil of SP(SPEO) on the proliferation of hepatocellular carcinoma cells and the possible mechanisms. The growth inhibition of Hep G2 cells was analyzed by MTT assay. Hoechst 33258 and fluorescence microscopy were utilized to observe the nuclear morphological changes of apoptotic cells. Flow cytometry was used to detect cell apoptosis and cell cycle. The expressions of the target proteins were detected by Western blotting. The results showed that SPEO obviously inhibited the proliferation of Hep G2 cells in a dose-dependent manner. SPEO activated a series of apoptotic proteins in Hep G2 cells, increasing expression levels of Bax, caspase-3 and caspase-9, and decreasing the bcl-2 expression level. SPEO displayed promising anti-hepatocellular carcinoma activities in vitro, partly by inducing apoptosis in Hep G2 cells through activating the mitochondrial pathway.
基金supported in part by the Sumitomo Foundation (Grant no. 083062)
摘要We investigated the culm form for one of the largest bamboo species, Phyllostachys pubescens Mazel ex Houz. in relation to the me- chanical constraint principles, i.e., elastic, stress and geometric similarity. The fine-resolution analysis of the culm taper indicated that the culm for P. pubescens consisted of three or four segments with various forms, except for the butt swell. This implied that the taper of the whole culm for P. pubescens could be expressed by neither of these principles. The regression slope between culm height and diameter at breast height on the double logarithmic coordinates was 0.629, which was significantly different from the values predicted from these principles. In conclusion, none of these mechanical constraint principles can express the culm taper and height-diameter relationship, and there may be a need for a more complicated model to express the culm form for P. pubescens.
摘要[Objective] This study aimed to understand the species and behaviors of flower-visiting insects on Mussaenda pubescens Ait.f.in the Mount Emei National Nature Reserve.[Method] The species,visiting frequency,visiting time and behaviors of flower-visiting insects on M.pubescens were studied by collection,identification and image capture.[Result] The flower-visiting insects on M.pubescens in the Mount Emei covered total 26 species with 21 species from Lepidoptera,2 species from Hymenoptera,and 3 species from Diptera.Thus,Lepidoptera were the main group of pollinators for c in the Mount Emei National Nature Reserve.The daily activity rhythms of Lepidoptera,Hymenoptera and Diptera showed different characteristics,and they were characterized by single-peak type and double-peak type.[Conclusion] This study will provide certain theoretical basis for studies on the diversity of flower-visiting insects on plants in the Mount Emei National Nature Reserve.
基金Supported by the State Key Development Program for Basic Research of China(2007CB210203)the Special Research Fund for the Doctoral Program of Higher Education of China(20050610013)
摘要A laboratory reactor was designed to test the effect of the interference instant of HY on the pyrolysis of pubescens.The time instant for intermediate species from pyrolysis to contact HY was controlled by varying the position of the catalyst bed relative to the pyrolytic cell.It was found that the effect of the interference instant was significant on the variation of different intermediate species,and the yield and quality of the products.The results also showed that,with the increase in the distance between the pyrolytic cell and the catalyst bed,the yield of liquid and relative content of the organics such as aldehyde,phenols,etc.,decreased,while the yield of residue and relative content of acetic acid increased.The deoxygenation of the intermediate species was favored when the catalyst exerted its performance on them immediately after their formation.
基金the Jiangsu Province Basic Facility Project(Nos.:BM2006104,BM2006507).
摘要A new angelol-type coumarin glucoside,6-[(1R,2R)-1,2-dihydroxy-3-β-D-glucosyloxy-3-methylbutyl]-7-methoxycoumarin,was isolated from the roots of Angelica pubescens.Its structure was elucidated on the basis of spectral analysis.
摘要Development of diabetic cataract is mainly associated with the accumulation of sorbitol via the polyol pathway through the action of Aldose reductase(AR).Hence,AR inhibitors are considered as potential agents in the management of diabetic cataract.This study explored the AR inhibition potential of Hemidesmus indicus var.pubescens root extract by in silico and ex vivo methods.Molecular docking studies(Auto Dock tool)betweenβ-sitosterol,hemidesminine,hemidesmin-1,hemidesmin-2,and AR showed thatβ-sitosterol(−10.2 kcal/mol)and hemidesmin-2(−8.07 kcal/mol)had the strongest affinity to AR enzyme.Ex vivo studies were performed by incubating isolated goat lenses in artificial aqueous humor using galactose(55 mM)as cataract inducing agent at room temperature(pH 7.8)for 72 h.After treatment with Vitamin E acetate−100μg/mL(standard)and test extract(500 and 1000μg/mL)separately,the estimation of biochemical markers showed inhibition of lens AR activity and decreased sorbitol levels.Additionally,extract also normalized the levels of antioxidant markers like SOD,CAT,GSH.Our results showed evidence that H.indicus var.pubescens root was able to prevent cataract by prevention of opacification and formation of polyols that underlines its potential as a possible therapeutic agent against diabetic complications.
摘要Objective:To evaluate the antioxidant and cytotoxic effects of hexane extract of Morinda pubescens leaves and to find the primary bioactive compound responsible for antioxidant and cytotoxic activities.Methods:The individual compounds were isolated using column chromatography and were characterized by spectroscopic techniques.The antioxidant activity was evaluated for all individual isolated compounds by DPPH method using L-Ascorbic acid as standard and cytotoxicity was assessed for the extract and the hyoscyamine by MTT assay, caspase test and RT-PCR study.Results:The antioxidant activity of the isolated compounds and the extract increased as the concentration increased.One of the isolated compound hyoscyamine showed the high antioxidant activity.The extract and the hyoscyamine dose-dependently decreased the cell viability in HepG2 cells.Hyoscyamine induced caspase-3 mediated apoptosis. Up regulation of p53 gene expression provides cue for apoptotic activity of hyoscyamine. Conclusions:The results indicate that hexane extract possessed potent antioxidant and cytotoxic activity and hyoscyamine is the principal bioactive compound in hexane extract.
基金Supported by Public Welfare Research Projects of Science and Technology Bureau of Hubei Province(40 2012DBA40001)~~
摘要Toona ciliata and Toona ciliata var. pubescens are timber species of national second-class protective plants. In China, Toona ciliata and Toona ciliata var.pubescens distribute sporadically with small population size but with great development potential. Plus selection on phenotype of Toona ciliata and Toona ciliata var.pubescens, molecular genetic marker of Toona ciliata var. pubescens, research progress in physiology and ecology of Toona ciliata and Toona ciliata var.pubescens were summarized in this paper. Suggestions and prospect forecast were proposed for relative research and utilization.
基金Supported by Forestry Science and Technology Plan of Hunan Province(XLK201925,XLK201806)National Forestry Science and Technology Development Project(KJZXSA2018011,KJZXSA2019009)Operational Subsidy Project of National Forestry Science and Technology Innovation Platform(2018-LYPT-DW-064)
摘要To accurately calculate carbon fixation capacity of P.pubescens forest in Hunan Province and promote the balanced and stable development of P.pubescens forestry in Hunan Province,standard sample plot was set in major producing area of P.pubescens in Hunan Province,and biomass of 258 P.pubescens was measured according to DBH and age class.Multivariate regression method was used to establish univariate (DBH as independent variable) and bivariate (DBH and age class as independent variables) models of total aboveground biomass and organ biomass of P.pubescens .Model evaluation and test showed that each model accorded with the applicable precision and had the appropriate prediction level.
基金Supported by Public Welfare Research Projects of Science and Technology Bureau of Hubei Province(40 2012DBA40001)~~
摘要Toona ciliata and Toona ciliata var. pubescens are rare timber species. In China, Toona ciliata and Toona ciliata var. pubescens distribute sporadical y with smal popuIation size but have huge deveIopment potential. The paper reviewed the research progress of their seedIing breeding, nutrient characteristics, fertiIization technoIogy, afforestation design, timber utiIization, chemistry and pharmacoIogy, and then Iooked into the future research and utiIization of Toona ciliata and Toona ciliata var. pubescens.