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Patterned line-illumination mesoscopy with a moving slit for enhancing background suppression in cortex-wide mouse brain imaging 认领 引用
1
作者 Chaowei Zhuang Yi Yang Hao Xie 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第2期679-684,共6页
Wide-field mesoscopy provides the capabilities of cortex-wide field of view(FOV),cellular resolution and high frame rate for neuronal imaging in the mouse brain.However,inherent background fluorescence degrades the im... Wide-field mesoscopy provides the capabilities of cortex-wide field of view(FOV),cellular resolution and high frame rate for neuronal imaging in the mouse brain.However,inherent background fluorescence degrades the image quality and hinders neuronal signal extraction.To address this problem,we first introduce a cortex-wide,high-resolution lineillumination mesoscope with a moving slit designed for in vivo mouse brain imaging.This system achieves a 6.6×6.6 mm FOV,microscale cellular resolution,a high frame rate of 10 Hz,as well as the background rejection ability.Furthermore,we integrated patterned illumination into the system to enhance the background suppression.Experimental results show that the proposed system successfully captures neurodynamics in the living mouse brain.Compared with conventional wide-field mesoscopes,the cortex-wide patterned line-illumination mesoscope(PLIM)achieves a threefold increase in the signal-to-background ratio(SBR).With patterned illumination integrated,the SBR enhancement further reaches four-anda-half-fold. 展开更多
关键词 wide-field fluorescence imaging optical sectioning patterned illumination
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Improved stability of amorphous InGaZnO4 thin-film transistors under negative bias illumination stress with the incorporation of fluorine passivation and metal shielding lines 认领 引用
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作者 Yuhan Feng Nannan Lv +2 位作者 Huaisheng Wang Mingxiang Wang Dongli Zhang 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第5期646-652,共7页
The instability phenomenon under negative bias illumination stress(NBIS)remains a major challenge for the application of amorphous indium gallium zinc oxide(a-IGZO)thin-film transistors(TFTs)in active-matrix displays.... The instability phenomenon under negative bias illumination stress(NBIS)remains a major challenge for the application of amorphous indium gallium zinc oxide(a-IGZO)thin-film transistors(TFTs)in active-matrix displays.In this paper,we employ fluorine plasma treatment and a segmented metal cover line approach to enhance the stability of elevated metal metal-oxide(EMMO)a-IGZO TFTs under NBIS.At room temperature,after 15 minutes of fluorine treatment,∆VONdecreases from 5.53 V to 2.02 V.This improvement is mainly attributed to the fact that fluorine atoms fill the ionized oxygen vacancies in a-IGZO,thereby reducing the density of defect states in the channel.Further adding 2.0µm wide metal-covered wires reduces the∆VONto 0.35 V.Under 80℃NBIS,the∆VONis limited to 3.79 V.This improvement is mainly attributed to the light-shielding effect of the metal lines and the passivation of oxygen vacancies by fluorine,thereby enhancing device stability under NBIS. 展开更多
关键词 thin-film transistor (TFT) a-IGZO negative bias illumination stress (NBIS) stability fluorination
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Interface reaction inhibition in phosphor-in-silica glass for high-performance laser illumination 认领 引用
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作者 Gengli Chen Shiyan Li +5 位作者 Wenli Zhou Zezhong Yang Pengyan Fu Liping Yu Shixun Lian Jing Wang 《Science China Materials》 SCIE EI CAS CSCD 2026年第8期4568-4577,共10页
To address the critical challenge of suppressing glass-phosphor interface reactions in high-melting-point phosphor-in-glass(PiG)for high-power laser illumination,this study fabricates a Y3Al5O12:Ce3^(+)(YA... To address the critical challenge of suppressing glass-phosphor interface reactions in high-melting-point phosphor-in-glass(PiG)for high-power laser illumination,this study fabricates a Y3Al5O12:Ce3^(+)(YAG:Ce)phosphor-insilica glass(YAG:Ce-PiSG)with high quantum efficiency and stability by regulating alkali metal oxides.Incorporation of Cs2O notably inhibits SiO2-YAG:Ce reactions,maintaining the PiSG’s internal quantum efficiency(IQE)at 97.7%of pure YAG:Ce(88.3%even after 1400℃calcination for 2 h),while smaller alkali ions(Li+,Na+)accelerate YAG:Ce decomposition.Cs2CO3acts as a flux to promote the melting of nanoSiO2,thereby facilitating the formation of a dense,transparent glass matrix.Owing to the large ionic radius of Cs+and its weak interaction with oxygen ions,the ready generation of non-bridging oxygen(NBO)is suppressed,consequently enabling the formation of a complete silica glass network that prevents alkali metal ions from etching the YAG:Ce phosphor.Leveraging the mixed alkali effect(10%Li2O+5%Cs2O),the PiSG retains excellent luminescence and resists 200℃hydrothermal treatment for 10 h.The PiG film-sapphire device delivers 3080 lm luminous flux and 213 lm W-1 efficiency under blue laser excitation.These findings demonstrate that the developed YAG:Ce-PiSG serves as a highly promising color-conversion material for high-performance laser illumination. 展开更多
关键词 phosphor-in-glass interface reaction inhibition mixed alkali effect high-power laser illumination color-conversion material
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Deep learning material recognition based on backscattering field of target with vortex beam illumination 认领 引用
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作者 Xingling FU Xiaoqi YANG +2 位作者 Fan JIA Wei LIN Bo LIU 《Optoelectronics Letters》 EI 2026年第5期309-313,共5页
In this work,a material recognition technology based on the backscattering field of a target with vortex beam illumination is proposed to meet the application requirements of target material recognition and classifica... In this work,a material recognition technology based on the backscattering field of a target with vortex beam illumination is proposed to meet the application requirements of target material recognition and classification in laser detection.Firstly,the characteristics of the backscattering light field of the target with vortex beam illumination are analyzed,and it is proved that the spatial frequency bandwidth of the specklegram increases with the increase of the topological charge of the vortex beam,so that the features of the specklegram will become more abundant.Subsequently,an experimental setup was built to record the backscattering specklegram and establish a dataset for validation.Six typical artificial neural networks(ANNs)were used to achieve the task of target material recognition.With a dataset of 1000 samples for each of three categories,the recognition accuracy can be up to 96.89%.Finally,a comprehensive evaluation model is established when we consider the factors,including recognition accuracy,model complexity,and training time,and the performances of these ANNs are compared.Among these ANNs,ResNet-18 exhibits superior overall performance.The proposed target material recognition technique paves a new way to multi-dimensional laser detection technology. 展开更多
关键词 target material recognition material recognition backscattering light field vortex beam illumination laser detectionfirstlythe backscattering field spatial frequency bandwidth material recognition technology
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Parameter optimization of the observation system for the South Yellow Sea strong shielding layer based on seismic illumination analysis 认领 引用 被引量：3
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作者 Yang Jia-Jia Chen Jian-Wen +5 位作者 Huang Fu-Qiang Yan Zhong-Hui Lei Bao-Hua Wang Xiao-Jie Xu Hua-Ning Liu Hong 《Applied Geophysics》 SCIE CSCD 2025年第1期84-98,233,共15页
The seismic data of the Laoshan Uplift in the South Yellow Sea Basin reveal a low signal-tonoise ratio and low refl ection signal energy in the deep Mesozoic–Paleozoic strata.The main reason is that the Mesozoic-Pale... The seismic data of the Laoshan Uplift in the South Yellow Sea Basin reveal a low signal-tonoise ratio and low refl ection signal energy in the deep Mesozoic–Paleozoic strata.The main reason is that the Mesozoic-Paleozoic marine carbonate rock strata are directly covered by the Cenozoic terrestrial clastic rock strata,which form a strong shielding layer.To obtain the reflection signals of the strata below the strong shielding layer,a one-way wave equation bidirectional illumination analysis of the main observation system parameters was conducted by analyzing the mechanism of the strong shielding layer.Low-frequency seismic sources are assumed to have a high illumination intensity on the reflection layer below the strong shielding layer.Accordingly,optimized acquisition parameter suggestions were proposed,and reacquisition was performed at the existing survey line locations in the Laoshan Uplift area.The imaging of the newly acquired data in the middle and deep layers was drastically improved.It revealed the unconformity between the Sinian and Cambrian under the strong shielding layer.The study yielded new insights into the tectonic and sedimentary evolution of the Lower Paleozoic in the South Yellow Sea. 展开更多
关键词 illumination analysis acquisition parameters Laoshan Uplift strong shielding layer
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Aberration-corrected differential phase contrast microscopy with annular illuminations 认领 引用 被引量：2
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作者 Yao Fan Chenyue Zheng +6 位作者 Yefeng Shu Qingyang Fu Lixiang Xiong Guifeng Lu Jiasong Sun Chao Zuo Qian Chen 《Opto-Electronic Science》 CAS 2025年第8期2-12,共11页
Quantitative phase imaging(QPI)enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes,revolutionizing label-free microscopy in cellular research.Differential phase ... Quantitative phase imaging(QPI)enables non-invasive cellular analysis by utilizing cell thickness and refractive index as intrinsic probes,revolutionizing label-free microscopy in cellular research.Differential phase contrast(DPC),a non-interferometric QPI technique,requires only four intensity images under asymmetric illumination to recover the phase of a sample,offering the advantages of being label-free,non-coherent and highly robust.Its phase reconstruction result relies on precise modeling of the phase transfer function(PTF).However,in real optical systems,the PTF will deviate from its theoretical ideal due to the unknown wavefront aberrations,which will lead to significant artifacts and distortions in the reconstructed phase.We propose an aberration-corrected DPC(ACDPC)method that utilizes three intensity images under annular illumination to jointly retrieve the aberration and the phase,achieving high-quality QPI with minimal raw data.By employing three annular illuminations precisely matched to the numerical aperture of the objective lens,the object information is transmitted into the acquired intensity with a high signal-to-noise ratio.Phase retrieval is achieved by an iterative deconvolution algorithm that uses simulated annealing to estimate the aberration and further employs regularized deconvolution to reconstruct the phase,ultimately obtaining a refined complex pupil function and an aberration-corrected quantitative phase.We demonstrate that ACDPC is robust to multi-order aberrations without any priori knowledge,and can effectively retrieve and correct system aberrations to obtain high-quality quantitative phase.Experimental results show that ACDPC can clearly reproduce subcellular structures such as vesicles and lipid droplets with higher resolution than conventional DPC,which opens up new possibilities for more accurate subcellular structure analysis in cell biology. 展开更多
关键词 quantitative phase imaging differential phase contrast aberration-corrected annular illumination
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Designing high-reflectivity AIN composite substrates by introduction of TiO2 to synthesize green/white converters for high-brightness reflective laser illumination 认领 引用 被引量：1
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作者 Qianxiong Wen Cong Zhao +7 位作者 Meilin Fu Yusai Xu Xiangjia Sun Jiaying Zheng Yanrong Liang Weidong Xiang Xiaojuan Liang Zhaoping Chen 《Journal of Rare Earths》 SCIE EI CAS CSCD 2025年第1期64-72,I0003,共9页
Phosphor-in-glass(PiG)has been prepared into various types of phosphor films owing to its simplicity process,exceptional color purity,and convenient color adjustability.Nevertheless,existing reflective PiGs films have... Phosphor-in-glass(PiG)has been prepared into various types of phosphor films owing to its simplicity process,exceptional color purity,and convenient color adjustability.Nevertheless,existing reflective PiGs films have encountered limitations in terms of stability and feasibility as reliable color converters,mainly attributed to issues related to thermal deposition and insufficient optical efficiency.Herein,we propose to use AlN substrate with superior thermal conductivity to coat the TiO2 layer to obtain TiO2-AlN(TA),which enhances the reflectivity of blue light to facilitate the light conversion process.By incorporating highly thermally stable LuAG:Ce-PiGs on a TA substrate,the LuAG:Ce-PiTA converter exhibits a luminous flux of 1102 lm@6.4 W,and maintains a relative intensity of 94.6%at 473 K benefiting from the high thermal conductivity of 34.1 W/(m·K).The addition of CASN3:Eu to the color converter 50 L&10C-PiTA enables an impressive CRI of 90.7.Relative lumine scence intensities of LuAG:Ce-PiTA and 50 L&10C-PiTA only decrease by 5.35%and 3.28%,respectively,in the 24 h illumination aging decay test of the reflective laser module.The results confirm the suitability of the optimally designed TA substrate for LuAG:Ce color converter applications in high-power reflective laser illumination. 展开更多
关键词 TiO2layer AIN substrate LuAG:Ce Color converter Laser illumination Rare earths
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High-thermal-conductivity YAGG:Ce color converters on AlN substrate enabling high power and superior luminance in static and dynamic reflective laser illumination 认领 引用 被引量：1
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作者 Jiaying Zheng Jiao Liu +6 位作者 Gaohong Wu Tengfei Tian Haitao Wang Jie Li Weiwei Huan Xiaojuan Liang Weidong Xiang 《Journal of Rare Earths》 SCIE EI CAS CSCD 2025年第12期2629-2636,I0002,共8页
Phosphor-in-glass(PiG)films have emerged as the preferred conversion materials for high-brightness laser-driven lighting due to their excellent thermal conductivity and superior optical performance.Screen printing tec... Phosphor-in-glass(PiG)films have emerged as the preferred conversion materials for high-brightness laser-driven lighting due to their excellent thermal conductivity and superior optical performance.Screen printing technology was employed to investigate the correlation between the reflective layer thickness and light conversion efficiency.A multilayer-structured Y3Al3.5Ga1.5O12:Ce3+(YAGG:Ce)-PiGTiO2-aluminium nitride(AIN)film(YG-TAF)converter was constructed by efficiently coupling the optimal thickness of the reflective layer with the PiG layer and the AIN substrate.Notably,the YG-TAF shows an impressive thermal conductivity of 22.6 W/(m·K)and a maximum anti-laser power of 15.84 W,demonstrating superb thermal regulation capability.This anti-laser power of 15.84 W represents a breakthrough in current static laser performance research.Impressively,an YG-TAF phosphor color wheel was designed,which achieves an ultra-high-brightness 4375 lm of green light under 450 nm,88 W laser power excitation,close to that of commercial phosphor silicone color wheels.This advancement not only demonstrates the excellent performance of YG-TAF in high-brightness dynamic reflective laser applications but also indicates its strong feasibility for practical implementation.Furthermore,the developed YAGG:Ce-Y1.31Ce0.09Gd1.6Al_5O12-TAF spliced phosphor color wheel successfully mitigates the influence of photon reabsorption and achieves a color rendering index of 80.5,showing great potential for advancement of the field of reflective white light laser illumination. 展开更多
关键词 YAGG:Ce color converter AlN substrate Phosphor color wheel Staic and dynamic reflective laser illumination Rare earths
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Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopy 认领 引用
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作者 Linbo Wang Simin Li +4 位作者 Xiaohu Chen Xin Jin Jie Zhang Hui Li Gang Wen 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2025年第4期121-131,共11页
Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope(SR-SIM).However,the imperfect optical... Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope(SR-SIM).However,the imperfect optical components can depolarize the laser beams hence degenerating the modulation depth.Here,we first presented a direct measurement method designed to estimate the modulation depth more precisely by shifting illumination patterns with equal phase steps.This measurement method greatly reduces the dependence of modulation depths on the samples,and then developed a polarization optimization method to achieve high modulation depth at all orientations by actively and quantitatively compensating for the additional phase difference using a combination of waveplate and a liquid crystal variable retarder(LCVR).Experimental results demonstrate that our method can achieve illumination patterns with modulation depth higher than 0.94 at three orientations with only one LCVR voltage,which enables isotropic resolution improvement. 展开更多
关键词 Structured illumination microscopy depolarization modulation depth phase compensation
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Local Content-Aware Enhancement for Low-Light Images with Non-Uniform Illumination 认领 引用
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作者 Qi Mu Yuanjie Guo +2 位作者 Xiangfu Ge Xinyue Wang Zhanli Li 《Computers, Materials & Continua》 SCIE EI 2025年第3期4669-4690,共22页
In low-light image enhancement,prevailing Retinex-based methods often struggle with precise illumina-tion estimation and brightness modulation.This can result in issues such as halo artifacts,blurred edges,and diminis... In low-light image enhancement,prevailing Retinex-based methods often struggle with precise illumina-tion estimation and brightness modulation.This can result in issues such as halo artifacts,blurred edges,and diminished details in bright regions,particularly under non-uniform illumination conditions.We propose an innovative approach that refines low-light images by leveraging an in-depth awareness of local content within the image.By introducing multi-scale effective guided filtering,our method surpasses the limitations of traditional isotropic filters,such as Gaussian filters,in handling non-uniform illumination.It dynamically adjusts regularization parameters in response to local image characteristics and significantly integrates edge perception across different scales.This balanced approach achieves a harmonious blend of smoothing and detail preservation,enabling more accurate illumination estimation.Additionally,we have designed an adaptive gamma correction function that dynamically adjusts the brightness value based on local pixel intensity,further balancing enhancement effects across different brightness levels in the image.Experimental results demonstrate the effectiveness of our proposed method for non-uniform illumination images across various scenarios.It exhibits superior quality and objective evaluation scores compared to existing methods.Our method effectively addresses potential issues that existing methods encounter when processing non-uniform illumination images,producing enhanced images with precise details and natural,vivid colors. 展开更多
关键词 Retinex non-uniform low illumination local content-aware effective guided image filtering
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Quantitative evaluation of intensity fidelity of superresolution reconstruction for structured illumination microscopy 认领 引用 被引量：1
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作者 Yujun Tang Linbo Wang +1 位作者 Gang Wen Hui Li 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2025年第1期153-163,共11页
Super-resolution structured illumination microscopy(SR-SIM)relies heavily on post-processing reconstruction to obtain high-quality SR images from raw data.Although many SIM reconstruction algorithms have been develope... Super-resolution structured illumination microscopy(SR-SIM)relies heavily on post-processing reconstruction to obtain high-quality SR images from raw data.Although many SIM reconstruction algorithms have been developed to recover fine cellular structures with high fidelity even from the noisy data,whether the pixel intensities of reconstructed SR images are still proportional to the original fluorescence intensity has been less explored.The linearity between the intensity before and after reconstruction is de fined as the intensity fidelity.Here,we proposed a method to evaluate the reconstructed SR image intensity fidelity at different spatial frequencies.With the proposed metric,we systematically investigated the impact of the key factors on the intensity fidelity in the standard Wiener-SIM reconstructions with simulated data,then evaluated the intensity fidelity of the SR images reconstructed by representative open-source packages.Our work provides a reference for SR-SIM image intensity fidelity improvement. 展开更多
关键词 Structured illumination microscopy super-resolution image reconstruction intensity fidelity spectrum optimization
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Low-light image enhancement based on multi-illumination estimation and multi-scale fusion 认领 引用 被引量：2
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作者 ZHANG Xin'ai GAO Jing +1 位作者 NIE Kaiming LUO Tao 《Optoelectronics Letters》 EI 2025年第6期362-369,共8页
To improve image quality under low illumination conditions,a novel low-light image enhancement method is proposed in this paper based on multi-illumination estimation and multi-scale fusion(MIMS).Firstly,the illuminat... To improve image quality under low illumination conditions,a novel low-light image enhancement method is proposed in this paper based on multi-illumination estimation and multi-scale fusion(MIMS).Firstly,the illumination is processed by contrast-limited adaptive histogram equalization(CLAHE),adaptive complementary gamma function(ACG),and adaptive detail preserving S-curve(ADPS),respectively,to obtain three components.Then,the fusion-relevant features,exposure,and color contrast are selected as the weight maps.Subsequently,these components and weight maps are fused through multi-scale to generate enhanced illumination.Finally,the enhanced images are obtained by multiplying the enhanced illumination and reflectance.Compared with existing approaches,this proposed method achieves an average increase of 0.81%and 2.89%in the structural similarity index measurement(SSIM)and peak signal-to-noise ratio(PSNR),and a decrease of 6.17%and 32.61%in the natural image quality evaluator(NIQE)and gradient magnitude similarity deviation(GMSD),respectively. 展开更多
关键词 adaptive detail preserving s curve contrast limited adaptive histogram equalization adaptive complementary gamma function low light image enhancement equalization clahe adaptive complementary gamma function acg multi scale fusion weight maps multi illumination estimation
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Spin-Polarized Two-Dimensional Electron Gas with Giant Rashba Splitting at the EuTiO3/KTaO3(111) Interface under Light Illumination 认领 引用
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作者 Yu-Chen Zhao Jian-Jie Li +8 位作者 Zhen Wang Tian-Lin Zhou Ming-Hang Li Wen-Xiao Shi Feng-Xia Hu Ji-Rong Sun Gang Li Yun-Zhong Chen Bao-Gen Shen 《Chinese Physics Letters》 SCIE EI CAS CSCD 2025年第10期186-193,共8页
The two-dimensional electron gas(2DEG)formed at the interface between two oxide insulators provides new opportunities for electronics and spintronics.The broken inversion symmetry at the heterointerface results in a R... The two-dimensional electron gas(2DEG)formed at the interface between two oxide insulators provides new opportunities for electronics and spintronics.The broken inversion symmetry at the heterointerface results in a Rashba spin-orbit coupling(RSOC)effect that enables the conversion between spin and charge currents.However,conducting oxide interfaces that simultaneously exhibit strong RSOC and high carrier mobility-a combination query for achieving high spin-to-charge inter-conversion efficiencies-remain scarce.Herein,we report a correlated 2DEG with giant Rashba splitting and high electron mobility in(111)-oriented EuTiO3/KTaO3(ETO/KTO)heterostructures under light illumination.Upon light modulation,a unique carrier-dependent giant anomalous Hall effect,the signature of spin-polarized 2DEG,emerges with a sign crossover at a carrier density of approximately 5.0×1013cm-2,highlighting dramatic changes in the band topology of KTO(111)interface.Furthermore,at 2 K,the carrier mobility is enhanced from 103 cm2·V-1·s-1to 1800 cm2·V-1·s-1,a remarkable enhancement of approximately 20 times.Accompanying with a giant Rashba coefficient αR up to 360meV·˚A,this high mobility ferromagnetic 5d oxide 2DEG is predicted to achieve a giant spin-to-charge conversion efficiency ofλ~10 nm,showing great potential for designing low-power spin-orbitronic devices. 展开更多
关键词 spin polarized giant Rashba splitting broken inversion symmetry conversion spin charge currentshoweverconducting oxide interfaces eutio KTaO interface light illumination oxide insulators electronics spintronicsthe
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Surface Measurement Based on Instantaneous Random Illumination 认领 引用 被引量：10
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作者 张辉 张丽艳 +1 位作者 王宏涛 陈鉴富 《Chinese Journal of Aeronautics》 SCIE EI CAS 2009年第3期316-324,共9页
This article introduces a surface measurement system which is able to take a measurement in an extremely short period of less than 2 milliseconds. It is composed of two cameras together with a specifically designed pr... This article introduces a surface measurement system which is able to take a measurement in an extremely short period of less than 2 milliseconds. It is composed of two cameras together with a specifically designed projector that projects an instantaneous random image pattern onto the objective surface. With the aid of the projected pattern, a match of image with corresponding sub-pixel is steadily established and a cluster of dense 3D points is obtained at the same time. A detailed discussion about the des... 展开更多
关键词 surface measurement stereo matching calibration instantaneous random illumination
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Improvement in Resolution of Multiphoton Scanning Structured Illumination Microscopy via Harmonics 认领 引用 被引量：4
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作者 Lei Wang Xiaomin Zheng +10 位作者 Jie Zhou Meiting Wang Jiajie Chen Youjun Zeng Gaixia Xu Ying Wang Haixia Qiu Yonghong Shao Junle Qu Bruce Zhi Gao Ying Gu 《Engineering》 SCIE EI CAS CSCD 2022年第9期65-72,共8页
We describe a multiphoton(mP)-structured illumination microscopy(SIM)technique,which demonstrates substantial improvement in image resolution compared with linear SIM due to the nonlinear response of fluorescence.This... We describe a multiphoton(mP)-structured illumination microscopy(SIM)technique,which demonstrates substantial improvement in image resolution compared with linear SIM due to the nonlinear response of fluorescence.This nonlinear response is caused by the effect of nonsinusoidal structured illumination created by scanning a sinusoidally modulated illumination to excite an mP fluorescence signal.The harmonics of the structured fluorescence illumination are utilised to improve resolution.We present an mP-SIM theory for reconstructing the super-resolution image of the system.Theoretically,the resolution of our m P-SIM is unlimited if all the high-order harmonics of the nonlinear response of fluorescence are considered.Experimentally,we demonstrate an 86 nm lateral resolution for two-photon(2P)-SIM and a 72 nm lateral resolution for second-harmonic-generation(SHG)-SIM.We further demonstrate their application by imaging cells stained with F-actin and collagen fibres in mouse-tail tendon.Our method can be directly used in commercial mP microscopes and requires no specific fluorophores or high-intensity laser. 展开更多
关键词 Super-resolution microscopy Structured illumination microscopy Multiphoton-structured illumination microscopy(SIM) SIM Second-harmonic-generation(SHG)-SIM
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Nonlinear scanning structured illumination microscopy based on nonsinusoidal modulation 认领 引用 被引量：5
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作者 Meiting Wang Lei Wang +6 位作者 Xiaomin Zheng Jie Zhou Jiajie Chen Youjun Zeng Junle Qu Yonghong Shao Bruce Zhi Gao 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2021年第5期25-32,共8页
Structured illumination microscopy(SIM)is an essential super-resolution microscopy technique that enhances resolution.Several images are required to reconstruct a super-resolution image.However,linear SIM resolution e... Structured illumination microscopy(SIM)is an essential super-resolution microscopy technique that enhances resolution.Several images are required to reconstruct a super-resolution image.However,linear SIM resolution enhancement can only increase the spatial resolution of micros-copy by a factor of two at most because the frequency of the structured illumination pattern is limited by the cutoff frequency of the excitation point spread function.The frequency of the pattern generated by the nonlinear response in samples is not limited;therefore,nonlinear SIM(NL-SIM),in theory,has no inherent limit to the resolution.In the present study,we describe a two-photon nonlinear SIM(2P-SIM)technique using a multiple harmonics scanning pattern that employs a composite structured illumination pattern,which can produce a higher order harmonic pattern based on the fluorescence nonlinear response in a 2P process.The theoretical models of super-resolution imaging were established through our simulation,which describes the working mechanism of the multi-frequency structure of the nonsinusoidal function to improve the reso-lution.The simulation results predict that a 5-fold improvement in resolution in the 2P-SIM is possible. 展开更多
关键词 Super-resolution image structured illumination microscopy nonsinusoidal function
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Method of lateral image reconstruction in structured illumination microscopy with super resolution 认领 引用 被引量：2
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作者 Qiang Yang Liangcai Cao +2 位作者 Hua Zhang Hao Zhang Guofan Jin 《Journal of Innovative Optical Health Sciences》 SCIE EI 2016年第3期4-18,共15页
The image reconstruction process in super-resolution structured illumination microscopy(SIM)is investigated.The structured pattern is generated by the interference of two Gaussian beams to encode undetectable spectra ... The image reconstruction process in super-resolution structured illumination microscopy(SIM)is investigated.The structured pattern is generated by the interference of two Gaussian beams to encode undetectable spectra into detectable region of microscope.After parameters estimation of the structured pattern,the encoded spectra are computationally decoded and recombined in Fourier domain to equivalently increase the cut-off frequency of microscope,resulting in the extension of detectable spectra and a reconstructed image with about two-fold enhanced resolution.Three di®erent methods to estimate the initial phase of structured pattern are compared,verifying the auto-correlation algorithm a®ords the fast,most precise and robust measurement.The artifacts sources and detailed reconstruction°owchart for both linear and nonlinear SIM are also presented. 展开更多
关键词 Microscopy structured illumination super-resolution image reconstruction
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Structured illumination microscopy and its new developments 认领 引用 被引量：2
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作者 Jianling Chen Caimin Qiu +3 位作者 Minghai You Xiaogang Chen Hongqin Yang Shusen Xie 《Journal of Innovative Optical Health Sciences》 SCIE EI 2016年第3期63-70,共8页
Optical microscopy allows us to observe the biological structures and processes within living cells.However,the spatial resolution of the optical microscopy is limited to about half of the wavelength by the light di&#... Optical microscopy allows us to observe the biological structures and processes within living cells.However,the spatial resolution of the optical microscopy is limited to about half of the wavelength by the light di®raction.Structured illumination microscopy(SIM),a type of new emerging super-resolution microscopy,doubles the spatial resolution by illuminating the specimen with a patterned light,and the sample and light source requirements of SIM are not as strict as the other super-resolution microscopy.In addition,SIM is easier to combine with the other imaging techniques to improve their imaging resolution,leading to the developments of diverse types of SIM.SIM has great potential to meet the various requirements of living cells imaging.Here,we review the recent developments of SIM and its combination with other imaging techniques. 展开更多
关键词 Structured illumination microscopy diffraction limit super-resolution
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High-speed autopolarization synchronization modulation three-dimensional structured illumination microscopy 认领 引用 被引量：14
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作者 Yaning Li Ruijie Cao +6 位作者 Wei Ren Yunzhe Fu Yiwei Hou Suyi Zhong Karl Zhanghao Meiqi Li Peng Xi 《Advanced Photonics Nexus》 2024年第1期1-10,共10页
In recent years,notable progress has been achieved in both the hardware and algorithms of structured illumination microscopy(SIM).Nevertheless,the advancement of three-dimensional structured illumination microscopy(3D... In recent years,notable progress has been achieved in both the hardware and algorithms of structured illumination microscopy(SIM).Nevertheless,the advancement of three-dimensional structured illumination microscopy(3DSIM)has been impeded by challenges arising from the speed and intricacy of polarization modulation.We introduce a high-speed modulation 3DSIM system,leveraging the polarizationmaintaining and modulation capabilities of a digital micromirror device(DMD)in conjunction with an electrooptic modulator.The DMD-3DSIM system yields a twofold enhancement in both lateral(133 nm)and axial(300 nm)resolution compared to wide-field imaging and can acquire a data set comprising 29 sections of 1024 pixels×1024 pixels,with 15 ms exposure time and 6.75 s per volume.The versatility of the DMD-3DSIM approach was exemplified through the imaging of various specimens,including fluorescent beads,nuclear pores,microtubules,actin filaments,and mitochondria within cells,as well as plant and animal tissues.Notably,polarized 3DSIM elucidated the orientation of actin filaments.Furthermore,the implementation of diverse deconvolution algorithms further enhances 3D resolution.The DMD-based 3DSIM system presents a rapid and reliable methodology for investigating biomedical phenomena,boasting capabilities encompassing 3D superresolution,fast temporal resolution,and polarization imaging. 展开更多
关键词 digital micromirror device electro-optic modulation polarization three-dimensional structured illumination microscopy
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DECREASING PITTING SUSCEPTIBILITY OF PASSIVE FILMS ON X70 PIPELINE STEEL IN NaCl SOLUTIONS BY ILLUMINATION 认领 引用 被引量：3
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作者 W.C. Zhu W.H. Leng +1 位作者 J.Q. Zhang C.N. Cao 《Acta Metallurgica Sinica(English Letters)》 EI CAS 2006年第2期91-97,共7页
The influence of UV illumination on passivity and pitting susceptibility on X70 pipoeline steel in a borate buffer （pH=8.4 ） solution containing NaCl is described. It is observed that illumination of the sample lead... The influence of UV illumination on passivity and pitting susceptibility on X70 pipoeline steel in a borate buffer （pH=8.4 ） solution containing NaCl is described. It is observed that illumination of the sample leads to a decrease in its pitting susceptibility as indicated by pitting potential and incubation time measurements in chloride containing electrolytes. This effect is strongly dependent on the applied potential during passivation. The electronic properties of the passive films on X70 steel were studied by Mott-Schottky analysis and photocurrent transient measurements. The results indicated that illumination during passivation led to modifications in the electronic properties of the passive films, mainly to a decrease of the bulk doping and an increase in the surface state density. The cause for the decrease in the pitting susceptibility is preliminary explained in terms of such modifications of the passive flm. 展开更多
关键词 X70 pipeline steel pitting susceptibility illumination passivation
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