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Engineered Lactobacillus reuteri for scavenging reactive oxygen species and modulating oral microflora in periodontitis therapy 认领 引用
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作者 Yuqiang Wang Ying Tang +6 位作者 Qianxiao Huang Jiaxin An Yueli Zhou Hongye Yang Fangfang Song Xianzheng Zhang Cui Huang 《International Journal of Oral Science》 SCIE CAS CSCD 2026年第3期488-503,共16页
The onset and progression of periodontitis are closely associated with subgingival dysbiosis and excessive localized oxidative stress.While some oral probiotics exhibit certain inhibitory effects on periodontitis-rela... The onset and progression of periodontitis are closely associated with subgingival dysbiosis and excessive localized oxidative stress.While some oral probiotics exhibit certain inhibitory effects on periodontitis-related pathogens,they often struggle to effectively colonize and antagonize these pathogens due to the complex oxidative stress at the site of periodontitis.In this study,we engineer Lactobacillus reuteri with a reactive oxygen species(ROS)-responsive adhesive polymer(phenylboric acid-dopamine-hyaluronic acid)(LR@PDH).In the periodontitis microenvironment,this polymer can consume ROS and then expose the phenolic hydroxyl group of dopamine,promoting the selective adhesion and colonization of Lactobacillus reuteri at the site of inflammation to antagonize pathogens.The results show that,compared to conventional probiotic therapy,inflammation-responsive adhesive Lactobacillus reuteri effectively alleviates local oxidative stress,reduces the abundance of pathogenic bacteria in the subgingival microbiome,and inhibits the progression of periodontitis.Additionally,its good biocompatibility and safety highlight its potential as a therapeutic approach for clinical treatment of periodontitis. 展开更多
关键词 oxidative stresswhile oral probiotics reactive oxygen reactive oxygen species engineered lactobacillus reuteri engineer lactobacillus reuteri subgingival dysbiosis oral microflora
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Reuterin-producing Limosilactobacillus reuteri with glycerol metabolism ameliorates Staphylococcus aureus-induced intestinal inflammation and dysfunction without gut microbiota dependent 认领 引用
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作者 Jinmei Li Kaixiang Zou +4 位作者 Xinrui Li Ke Zhu Yijuan Li Hua Wei Zhihong Zhang 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第2期823-835,共13页
Limosilactobacillus reuteri is a vertebrate symbiont that is widely appreciated as being of significant ecological importance for human health.As a unique feature,L.reuteri converts glycerol to the antimicrobial compo... Limosilactobacillus reuteri is a vertebrate symbiont that is widely appreciated as being of significant ecological importance for human health.As a unique feature,L.reuteri converts glycerol to the antimicrobial compound reuterin using enzymes encoded in its propanediol-utilization operon and evolves with host-driven diversification.Reuterin-producing L.reuteri HLRE13 was selectively isolated from poultry previously and confirmed to inhibit the growth of Staphylococcus aureus in vitro.However,it remains unclear whether L.reuteri HLRE13 retains these antagonistic properties when ingested in specific-pathogen-free mice.Here,we investigated the ameliorative effects and potential mechanisms of action of L.reuteri HLRE13 in combination with glycerol on S.aureus-induced infection phenotypes in mice.Firstly,our results confirmed that L.reuteri HLRE13 effectively inhibited the intestinal colonization of S.aureus CMCC26003;Secondly,L.reuteri HLRE13 combined with glycerol could alleviate the intestinal tissues damage caused by S.aureus through increasing the expression of ZO-1,Occludin,and MUC-2,ameliorate the intestinal systemic inflammatory response,and maintain the balance of gut microbiota by increasing the relative abundance of Lactobacillus and reducing the relative abundance of Staphylococcus.Furthermore,the colonization resistance was also found on L.reuteri HLRE13 combined with glycerol against S.aureus in pseudo germ-free mice,and they exerted the similar effects on alleviating intestinal damage and improving immune function.Combining these results,we speculate that reuterin-producing L.reuteri antagonize S.aureus in mice without the gut microbiota-dependent manner.Overall,our findings will provide a theoretical foundation for the scientific cognition of L.reiteri in maintaining intestinal health by producing reuterin. 展开更多
关键词 Limosilactobacillus reuteri Glycerol Reuterin Colonization resistance Intestinal barrier Gut microbiota
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Investigating the interactive adhesion ability between Limosilactobacillus reuteri DSM17938 and HT-29 cells regulated by soybean proteins and soybean peptides 认领 引用
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作者 Yinxiao Zhang Chi Zhang +3 位作者 Jingyi Wang He Li Xinqi Liu Xianyong Liu 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第5期2198-2206,共9页
In this study,digested soybean protein(dspr)and peptides(dspe)were found to have different effects on the adhesion ability of Limosilactobacillus reuteri DSM17938 and HT-29 cells.Results found that dspr and dspe could... In this study,digested soybean protein(dspr)and peptides(dspe)were found to have different effects on the adhesion ability of Limosilactobacillus reuteri DSM17938 and HT-29 cells.Results found that dspr and dspe could increase L.reuteri adhesion ability by affecting their auto-aggregation and hydrophobicity during their stationary phase.The moonlighting protein may play a dominant role affected by dspe in the adhesion process of L.reuteri from stationary phase.Moreover,dspr and dspe also enhanced the adhesion ability of HT-29cells to L.reuteri DSM17938,and they might play different role at different stages of adhesion.dspr mainly promoted the expression of adhesion-related genes before L.reuteri adhesion.dspe increased them after the adhesion of L.reuteri from log phase and dspr mainly enhanced their expression after stationary phase L.reuteri adhesion.Soybean protein and peptides may therefore be considered as a potential effective modulator of L.reuteri adhesion to intestinal tract. 展开更多
关键词 Soybean protein Soybean peptides Limosilactobacillus reuteri DSM17938 Adhesion Surface protein
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Limosilactobacillus reuteri CCFM1132 modulates gut microbiota and alleviates hyperuricemia:a randomized,double-blind,placebo-controlled trial 认领 引用
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作者 Lei Sun Yang Cao +6 位作者 Feng Hang Dongbo Yuan Honglun Wang Jianxin Zhao Gang Wang Guohong Qiao Liyun Wang 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第1期55-66,共12页
This randomized,double-blind,placebo-controlled trial evaluated the uric acid(UA)-lowering effect of Limosilactobacillus reuteri CCFM1132 in young males with hyperuricemia.Participants received 1×1010CFU of L.... This randomized,double-blind,placebo-controlled trial evaluated the uric acid(UA)-lowering effect of Limosilactobacillus reuteri CCFM1132 in young males with hyperuricemia.Participants received 1×1010CFU of L.reuteri CCFM1132(n=34)or placebo(n=31)daily for 8 weeks.After the intervention,serum UA concentration significantly decreased,along with a reduction in xanthine oxidase(XOD)activity compared to the placebo group(P<0.01).Indicators of liver(aspartate transaminase and alanine transaminase)and renal(urea and creatinine)functions improved.Short-chain fatty acid(SCFA)concentrations significantly increased,with an upregulated abundance of SCFA producers(Fusicatenibacter,Ruminococcaceae UCG_014,and Ruminococcus 1)in the gut.Additionally,correlation analysis revealed that concentrations of SCFAs,particularly acetate and butyrate,were strongly negatively correlated with UA concentration and XOD activity.These findings suggest that L.reuteri CCFM1132 relieves hyperuricemia by enhancing the abundance of SCFA producers in the gut to promote SCFA production and by suppressing XOD activity.This study provides a valuable reference for developing new treatments for hyperuricemia. 展开更多
关键词 Hyperuricemia Limosilactobacillus reuteri Xanthine oxidase Short-chain fatty acid Gut microbiota
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含Reuterin罗伊氏乳杆菌发酵乳的制备及其抗菌特性研究 认领 引用
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作者 朱可 李新瑞 +2 位作者 周梦雨 魏华 张志鸿 《食品工业科技》 EI CAS 北大核心 2026年第1期112-119,共8页
为制备一种可原位产生抗菌物质的益生菌发酵乳,本文以能代谢甘油产广谱抗菌物质Reuterin的罗伊氏乳杆菌HLRE05为发酵菌,探究制备发酵乳的最适条件,评价甘油的添加对Reuterin产生及发酵乳特性的影响,分析发酵乳贮藏期内的生化指标和Reute... 为制备一种可原位产生抗菌物质的益生菌发酵乳,本文以能代谢甘油产广谱抗菌物质Reuterin的罗伊氏乳杆菌HLRE05为发酵菌,探究制备发酵乳的最适条件,评价甘油的添加对Reuterin产生及发酵乳特性的影响,分析发酵乳贮藏期内的生化指标和Reuterin含量变化,并对含Reuterin发酵乳的抑菌能力进行评估。结果表明:含Reuterin发酵乳的制备条件可选择接种量为5%(YO-MIX 495:HLRE05=1:1)、糖含量为6%(鼠李糖:蔗糖=1:1)和发酵温度为37℃;当甘油的添加量为100 mmol/L时,原位产生Reuterin的含量达0.28 mg/mL,且其罗伊氏乳杆菌含量和持水性较好;另外,含Reuterin发酵乳在贮藏期内也能够保持较好的持水性,稳定的pH以及较高的Reuterin浓度,并能有效抑制病原微生物繁殖。该研究将为特色乳酸菌(如罗伊氏乳杆菌HLRE05)资源挖掘及其在乳制品中的应用奠定基础。 展开更多
关键词 罗伊氏乳杆菌 Reuterin 发酵乳 抗菌 贮藏期
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Strengthening causal inference and analytical rigor in the Wumei Pills-Lactobacillus reuteri-intestinal stem cell axis for chemotherapyinduced mucositis 认领 引用
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作者 Rui-Li Yang Qing Lu +1 位作者 Er-Min Liang Hong-Cheng Luo 《World Journal of Stem Cells》 SCIE 2026年第1期97-101,共5页
A recent preclinical study reported that Wumei Pills(WMP)and Lactobacillus reuteri(L.reuteri)mitigate 5-fluorouracil-induced intestinal mucositis by promoting intestinal stem cell(ISC)-mediated repair via Wnt/β-caten... A recent preclinical study reported that Wumei Pills(WMP)and Lactobacillus reuteri(L.reuteri)mitigate 5-fluorouracil-induced intestinal mucositis by promoting intestinal stem cell(ISC)-mediated repair via Wnt/β-catenin signaling.The mechanistic interpretation rests largely on systemic inflammation readouts,correlative microbiota changes,and immunohistochemistry of pathway markers.From a clinical standpoint,chemotherapy-induced mucositis remains a common and burdensome toxicity that leads to dose reductions,treatment delays,and infection risk;current care is largely supportive and does not directly restore ISCmediated repair.This unmet need motivates rigorous appraisal of the proposed“WMP→L.reuteri→ISC/Wnt”axis.To highlight key methodological considerations that may affect causal inference and analytical rigor in the proposed“WMP→L.reuteri→ISC/Wnt”pathway.This letter critically appraises the study’s design,endpoints,and analyses against current best practices in mucositis biology,microbiome causality testing,Wnt/β-catenin pathway validation,and preclinical statistics,and synthesizes concrete,literature-grounded remedies.Six issues with potential impact on interpretation were identified:(1)Reliance on serum cytokines/lipopolysaccharide to infer local mucosal inflammation,with limited tissue-level indices(e.g.,myeloperoxidase,interleukin-1β,immune-cell infiltration);(2)Absence of necessity/sufficiency tests to verify microbiota mediation(e.g.,L.reuteri depletion,WMP-donor fecal microbiota transplantation,probiotic add-back);(3)Pathway evidence tiering-Wnt/β-catenin activation not confirmed byβ-catenin nuclear translocation or downstream targets(Axin2,c-Myc,cyclin D1),and Lgr5 quantification/specificity insufficient;(4)Statistical design under-specified(power justification,blinded assessment,control of multiple comparisons)and potential cage effects unmodeled;(5)Limited dose-response and safety profiling for WMP/L.reuteri;and(6)Constrained generalizability(single sex/strain/age,lack of ABX-only controls,single time-point).The reported benefits of WMP and L.reuteri in chemotherapy-induced mucositis are promising,but stronger causal and analytical foundations are needed.Incorporating tissue-level inflammation readouts,microbiota loss-/gain-offunction designs,definitive Wnt/β-catenin activation assays,rigorous statistical practices(including mixed-effects models for cage clustering and multiplicity control),dose-response/safety evaluation,and broader experimental scope(sex/age/strain,ABX-only controls,time-course)will yield more robust and translationally relevant conclusions. 展开更多
关键词 Chemotherapy-induced mucositis Wumei Pills Lactobacillus reuteri Intestinal stem cells Wnt/β-catenin
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Dietary Qiwenghuangbo powder-enriched Limosilactobacillus reuteri protects the intestinal epithelium and alleviates inflammation via a strain- specific mechanism 认领 引用 被引量：1
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作者 Qian Xu Lei Xue +9 位作者 Zhenhua Wu Shuaishuai Kang Jia Li Yifan Wu Yujun Wu Jinbiao Zhao Rujuan Wu Huiyuan Lv Junjun Wang Dandan Han 《Animal Models and Experimental Medicine》 CAS CSCD 2025年第6期990-1003,共14页
Background:Qiwenghuangbo powder(QP),composed of Astragalus,Phellodendron chinensis,and Radix pulsatilla,is a traditional Chinese herbal formula,but its effects on weaned piglets remained unclear.Methods:Weaned piglets... Background:Qiwenghuangbo powder(QP),composed of Astragalus,Phellodendron chinensis,and Radix pulsatilla,is a traditional Chinese herbal formula,but its effects on weaned piglets remained unclear.Methods:Weaned piglets fed with 0.5 kg QP(QP1),1 kg QP(QP2),low-zinc oxide(ZnO;negative control),and high-ZnO(positive control)diets in two phases,respec-tively,and the growth performance,intestinal morphology,cytokines,and microbial communities were profiled.The mouse models of colitis induced by Citrobacter roden-tium and dextran sulfate sodium(DSS)were employed to elucidate the potential role of QP-fed enriched key species.Results:Dietary 1.0 kg QP alleviated diarrhea and inflammation and improved intes-tinal development and growth performance of weaned piglets.Moreover,this dietary intervention notably altered microbiota composition,characterized by the enrich-ment of Limosilactobacillus reuteri.Furthermore,out of three isolated L.reuteri,two strains could alleviate pathogen infection and intestinal inflammation,respectively.Specifically,the anti-inflammatory effect of one strain was achieved by promoting the colonization resistance of C.rodentium as significantly reduced pathogen loads.The other strain mitigated DSS-induced colitis by enhancing the goblet cell function and inhibiting the secretion of pro-inflammatory cytokines,particularly interleukin-1β(IL-1ß)and tumor necrosis factor-α(TNF-α).Conclusions:Dietary QP improved the growth performance and intestinal health of weaned piglets by promoting the colonization of L.reuteri.The isolated commensal L.reuteri control colitis in a strain-specific mechanism,highlighting the potential of QP and L.reuteri in providing evidence for gut health promotion. 展开更多
关键词 gut microbiome intestinal inflammation Limosilactobacillus reuteri Qiwenghuangbo powder,weaned piglets
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Wumei Pills enhance intestinal stem cell-mediated repair in chemotherapy-induced mucositis via Lactobacillus reuteri-dependent modulation of the gut microbiota 认领 引用 被引量：1
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作者 Dong-Xue Lu Zi-Xuan Wang +3 位作者 Xi-Min Liu Hua Wu Li-Jiang Ji Jing Yan 《World Journal of Stem Cells》 SCIE 2025年第9期151-162,共12页
BACKGROUND Intestinal mucositis is a severe and common complication of chemotherapy,characterized by disruption of the gut microbiota,intestinal inflammation,and epithelial barrier damage.Intestinal stem cells(ISCs)ar... BACKGROUND Intestinal mucositis is a severe and common complication of chemotherapy,characterized by disruption of the gut microbiota,intestinal inflammation,and epithelial barrier damage.Intestinal stem cells(ISCs)are essential for epithelial renewal and barrier maintenance,yet chemotherapy impairs ISC proliferation and function,delaying mucosal repair.We hypothesized that Wumei Pills(WMP)could protect against chemotherapy-induced intestinal mucositis by modulating gut microbiota-particularly Lactobacillus reuteri(L.reuteri)-to restore ISC activity,preserve microbial balance,reduce inflammation,and promote epithelial regeneration.AIM To characterize these changes and the safety of WMP via a 5-fluorouracil(5-FU)induced intestinal mucositis mouse model.METHODS In this study,we established a 5-FU induced intestinal mucositis mouse model,to explore the protective effect of WMP regulating L.reuteri on integrity of intestinal mucosa.RESULTS We found that intestinal flora is an important mechanism causing chemotherapyinduced intestinal mucositis,but WMP and live L.reuteri were effective in protecting the morphology of intestinal mucositis and normal proliferation of epithelial.L.reuteri colonized in the intestinal mucosa and WMP ameliorated intestinal mucosa damage caused by 5-FU treatment,including improvement of body weight,pathological change,and proliferation level,reducement proinflammatory cytokine secretion(tumor necrosis factor-α,interleukin-6)and the lipopolysaccharides concentration in serum.The repair process stimulated by both L.reuteri and WMP were also accompanied with increased leucine-richrepeat-containing G-protein-coupled receptor 5(+)and proliferating cell nuclear antigen of mice intestine.Furthermore,we demonstrated that WMP and L.reuteri activated the Wingless-type/β-catenin pathway to accelerate proliferation of intestinal epithelial,thus recovering damaged intestinal mucosa.However,the relieving effect of L.reuteri on intestinal mucosa was inferior to that of WMP.CONCLUSION Our findings indicate that WMP regulating L.reuteri protects intestinal barrier and activates intestinal epithelial proliferation,which sheds light on treatment approaches for intestinal inflammation based on ISCs with traditional Chinese medicine and probiotics L.reuteri. 展开更多
关键词 Wumei Pills Lactobacillus reuteri Intestinal stem cells Leucine-rich-repeatcontaining G-protein-coupled receptor 5 Wingless-type/β-catenin pathway
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The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice 认领 引用
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作者 Ce Qi Haowen Zhang +4 位作者 Zhenli Liu Xiaolin Liu Lintao Zhang Liangliang You Jin Sun 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第5期1776-1790,共15页
Limosilac to bacillus reuteri QS01 is potential probiotic isolated from the intestinal microbiota of healthy women in early pregnancy.In the current study,we examined whether QS01 can prevent gestational diabetes mell... Limosilac to bacillus reuteri QS01 is potential probiotic isolated from the intestinal microbiota of healthy women in early pregnancy.In the current study,we examined whether QS01 can prevent gestational diabetes mellitus(GDM) in an enhanced mouse model of the condition.Female C57BL/6J mouse offspring(F1 generation) born to dams fed a control(CON) or low-protein diet(GDM group) during gestation and lactation were used.Pregnant F1 mice fed a standard diet were randomly assigned to 5 groups:CON,GDM,and GDM mice treated with metformin,QS01 or Lacticaseibacillus rhamnosus HN001.An oral glucose tolerance test was performed before sacrifice at gestational day 17.Glucose tolerance was significantly ameliorated by all 3 treatments.QS01 supplementation fortified the intestinal mucosal barrier and inhibited the escalation of plasma inflammatory cytokines.QS01 treatment altered the cecal microbiota composition and function.Plasma and cecal metabolite profiles were modulated by QS01,prominently demonstrating significant upregulation of indole lactate and L-tryptophan in plasma and 5-hydroxy-tryptophan in the cecum,positively correlated with gut Lactobacillus abundance.In summary,QS01 plays a role in preventing GDM by remodeling the intestinal microbiota,reinforcing the intestinal mucosal barrier and alleviating chronic inflammation. 展开更多
关键词 Estational diabetes mellitus Limosilactobacillus reuteri Gut microbiota Mucosal barrier Tryptophan metabolism
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Anti-Photoaging Activities of Limosilactobacillus reuteri Culture Broth 认领 引用
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作者 Nu Ri Song Seo Yeon Shin +5 位作者 Ki Min Kim Sa Rang Choi Doo Sang Park Sun Oh Kim Dai Hyun Jung Kyung Mok Park 《BIOCELL》 SCIE 2025年第7期1291-1310,共20页
Objectives:Limosilactobacillus reuteri is a beneficial Lactobacillus widely used in foods and supplements to promote overall health.Some studies also suggest it supports skin health and prevents allergies and cardiova... Objectives:Limosilactobacillus reuteri is a beneficial Lactobacillus widely used in foods and supplements to promote overall health.Some studies also suggest it supports skin health and prevents allergies and cardiovascular disease.However,research on its skin-protective effects against photoaging has not been conducted.This study evaluated the potential of culture broths fromthree L.reuteri strains(DS0333,DS0384,and DS0385)to inhibit skin photoaging.Methods:To assess their anti-photoaging potential,the culture broths were examined for antioxidant capacity,melanin inhibition,and collagen synthesis promotion.Radical scavenging activity was tested using 2,2-diphenyl-1-picrylhydrazyl(DPPH)and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)assays.The biosynthetic activity of melanin and associated protein markers involved in melanogenesis was examined in a B16F10 mouse melanoma model.Type I procollagen synthesis and matrix metalloproteinase-1(MMP-1)inhibition were evaluated in ultraviolet B(UVB)-damaged human dermal fibroblasts(HDFs).Results:The culture broths exhibited concentrationdependent antioxidant activity and significantly suppressed melanin synthesis triggered byα-melanocyte-stimulating hormone(α-MSH).Transcription factors involved inmelanogenesis,namelymicrophthalmia-associated transcription factor(MITF),tyrosinase-related protein 1(TRP-1),and 2(TRP-2),were significantly downregulated following treatment.Treatment with culture broths also enhanced type I procollagen production and inhibited MMP-1 activity and protein expression in UVB-exposed HDFs.Among the strains,DS0333 demonstrated the strongest efficacy and was further investigated.It enhanced the proliferation of skin cells and attenuated the levels of age-associated markers such as MMP-1,mitogen-activated protein kinase(MAPK),and activator protein 1(AP-1).High-performance liquid chromatography(HPLC)analysis identified phenyllactic acid(PLA)as the predominant active compound.Conclusions:These results indicate that DS0333 culture broth exhibits strong anti-aging effects and can be applied in functional cosmetics aimed at promoting skin health. 展开更多
关键词 Limosilactobacillus reuteri probiotics culture broth anti-photoaging UVB
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Limosilactobacillus reuteri SXDT‑32‑derived shikimic acid protects against colonic inflammation in piglets by inhibiting the PI3K‑Akt pathway 认领 引用
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作者 Ying Chen Chengzeng Luo +9 位作者 Zhaohan Zhan Shuo Liu Chunran Teng Ruixiao Mao Shunfen Zhang Xunbozan Zhang Qingshi Meng Ruqing Zhong Liang Chen Hongfu Zhang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2341-2355,共15页
Background Colitis caused by bacterial infection is a major global health challenge.Unfortunately,current treatment options are limited.We previously disclosed that L.reuteri SXDT-32 was enriched in the feces of an an... Background Colitis caused by bacterial infection is a major global health challenge.Unfortunately,current treatment options are limited.We previously disclosed that L.reuteri SXDT-32 was enriched in the feces of an ancient diarrhearesistant pig breed(Mashen pig)in China over 2500 years old.As diarrhea is often closely associated with intestinal inflammation,L.reuteri SXDT-32 was identified as a potential beneficial bacterium to prevent intestinal inflammation.However,the precise mechanisms involved remained unclear.Results Our tests showed that L.reuteri SXDT-32 alleviated colonic damage induced by pathogenic E.coli SKLAN202302 in weaned pigs by enhancing barrier integrity and inhibiting inflammation.The transcriptomics revealed that L.reuteri SXDT-32 protected against inflammatory injury by inhibiting the PI3K-AKT signaling pathway.Metabolite analysis indicated that the content of shikimic acid(SA)was substantially elevated in the colonic mucosa of L.reuteri SXDT-32-fed piglets(P<0.05).In addition,Liquid Chromatography-Mass Spectrometer(LC-MS)analysis showed significant increases in SA content in both the colonic chyme of L.reuteri SXDT-32-fed piglets and the supernatant of in vitro grown cultures of L.reuteri SXDT-32(P<0.05).Polymerase chain reaction(PCR)analysis identified gene aroE from L.reuteri SXDT-32,which is a key gene directly linked to SA synthesis,and elevated shikimate dehydrogenase(SD,encoded by aroE)was also detected in both L.reuteri SXDT-32 and the colonic mucosa of piglets fed L.reuteri SXDT-32(P<0.01).In vitro Caco-2 cell experiments demonstrated that SA,L.reuteri SXDT-32,and the supernatant from in vitro grown cultures of L.reuteri SXDT-32 exhibited comparable inhibitory effects on the PI3K-Akt pathway to those of the PI3K inhibitor LY294002.Conclusions L.reuteri SXDT-32 alleviated intestinal inflammation in piglets by producing SA that inhibits the PI3K-Akt pathway.This study provides an innovative approach for the treatment and prevention of colitis caused by bacterial infection. 展开更多
关键词 Intestinal inflammation L.reuteri SXDT-32 PI3K-Akt pathway Shikimic acid
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Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process 认领 引用
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作者 陈国 杨道茂 +1 位作者 肖雅琴 陈宏文 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2011年第6期1023-1027,共5页
3-Hydroxypropionaldehyde （3-HPA） is a potential valuable chemical and new broad-spectrum antim-icrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from gly... 3-Hydroxypropionaldehyde （3-HPA） is a potential valuable chemical and new broad-spectrum antim-icrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concentration, biomass con-centration, pH and temperature on the production of 3-HPA by Lactobacillus reuteri CG001, respectively. The re-sults showed that molar conversion yield of 3-HPA/glycerol reached 97.9% under the condition that 200 mmol·L-1 glycerol was converted by 25.3 g·L-1 resting cell for 1 h at 30 ℃. The cells could not be reused directly because the L. reuteri almost lost its bioconversion activity completely, but the ability of glycerol conversion could gradually recover if the fresh medium was added to the deactivated cell for 4 h. 展开更多
关键词 Lactobacillus reuteri 3-hydroxypropionaldehyde reuterin bioconversion
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Effects of drinking water supplementation with Lactobacillus reuteri,and a mixture of reuterin and microcin J25 on the growth performance,caecal microbiota and selected metabolites of broiler chickens 认领 引用 被引量：5
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作者 Liya Zhang Laila Ben Said +3 位作者 Nadège Hervé Séverine Zirah Moussa Sory Diarra Ismail Fliss 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第4期1209-1221,共13页
Background:Since the overuse of antibiotics in animal production has led to a selection of antibiotic-resistant pathogens that affect humans and animals as well.Scientists are therefore searching for novel natural alt... Background:Since the overuse of antibiotics in animal production has led to a selection of antibiotic-resistant pathogens that affect humans and animals as well.Scientists are therefore searching for novel natural alternatives to antibiotics.In this study Lactobacillus reuteri and a combination of reuterin and microcin J25(RJ)were evaluated as promoters of growth and modulators of the cecal microbiota and metabolite profiles in broiler chickens.Oneday-old Cobb 500 male broilers were distributed to 8 treatments:negative control(without antibiotic),positive control(bacitracin),three concentrations of RJ and three doses of L.reuteri plus glycerol.The birds(2176,34 per pen,8 pens per treatment)were reared for 35 d.Results:The body weight of the bacitracin and 5 mmol/L reuterin combined with 0.08μmol/L microcin J25(10RJ)treatment group was significantly higher than that of the negative control group(P<0.05).L.reuteri had no significant effect on broiler growth.MiSeq high-throughput sequencing of 16S rRNA showed clustering of cecal microbial operational taxonomic unit diversity according to treatment.The influence of bacitracin and 10RJ on bacterial community overall structure was similar.They promoted Ruminococcaceae,Lachnospiraceae and Lactobacillaceae,increased the relative abundance of Faecalibacterium and decreased the abundance of Bacteroides and Alistipes,while the negative control condition favored Bacteroidaceae and Rikenellaceae.Furthermore,10RJ increased the concentration of short-chain fatty acid in the cecum and changed the metabolome overall.Conclusions:These overall suggest that 10RJ can promote a host-friendly gut environment by changing the cecal microbiome and metabolome.This combination of natural antimicrobial agents in the drinking water had a positive effect on broiler growth and may be suitable as an alternative to antibiotic growth promoters. 展开更多
关键词 Broiler chicken Lactobacillus reuteri Metabolome Microbiome Microcin J25 Reuterin SCFA
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Study and use of the probiotic Lactobacillus reuteri in pigs:a review 认领 引用 被引量：57
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作者 Chengli Hou Xiangfang Zeng +2 位作者 Fengjuan Yang Hong Liu Shiyan Qiao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2016年第1期26-33,共8页
Probiotics are living microorganisms that provide a wide variety of health benefits to the host when ingested in adequate amounts. The bacterial strains most frequently used as probiotic agents are lactic acid bacteri... Probiotics are living microorganisms that provide a wide variety of health benefits to the host when ingested in adequate amounts. The bacterial strains most frequently used as probiotic agents are lactic acid bacteria, such as Loctobocillus reuteri, which is one of the few endogenous Lactobadllus species found in the gastrointestinal tract of vertebrates, including humans, rats, pigs and chickens. L. reuteri is one of the most well documented probiotic species and has been widely utilized as a probiotic in humans and animals for many years. Initially, L. reuteri was used in humans to reduce the incidence and the severity of diarrhea, prevent colic and necrotic enterocolitis, and maintain a functional mucosal barrier. As interest in alternatives to in-feed antibiotics has grown in recent years, some evidence has emerged that probiotics may promote growth, improve the efficiency of feed utilization, prevent diarrhea, and regulate the immune system in pigs. In this review, the characteristics of L. reuteri are described, in order to update the evidence on the efficacy of using L. reuteri in pigs. 展开更多
关键词 Antibiotics Application Loctobocillus reuteri Pigs Probiotics
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产Reuterin罗伊氏乳杆菌的定向筛选及其生物学特性 认领 引用 被引量：9
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作者 李一娟 邵开生 +5 位作者 张娜 孟迎平 陶雪莹 周颖钰 魏华 张志鸿 《食品科学》 EI CAS CSCD 北大核心 2021年第14期32-38,共7页
依据菌株宿主适应性特点,从健康家禽新鲜粪便中定向筛选具有开发潜力的罗伊氏乳杆菌。经16S rDNA序列分析和pduC基因筛查,获得3株产抗菌物质Reuterin的罗伊氏乳杆菌,对其耐受模拟胃肠道环境、抗生素敏感性、甘油发酵上清液抑菌活性及肠... 依据菌株宿主适应性特点,从健康家禽新鲜粪便中定向筛选具有开发潜力的罗伊氏乳杆菌。经16S rDNA序列分析和pduC基因筛查,获得3株产抗菌物质Reuterin的罗伊氏乳杆菌,对其耐受模拟胃肠道环境、抗生素敏感性、甘油发酵上清液抑菌活性及肠上皮细胞黏附能力等生物学特性进行系统评价。结果表明,罗伊氏乳杆菌WLRE01、WLRE03、WLRE04均具有较强耐受极端酸(pH 2.5和pH 3.5)、胆盐(1.5 g/L)及模拟胃肠液的能力;经1 mmol/L过氧化氢处理6 h,WLRE03、WLRE04的活菌数能基本维持初始浓度;甘油发酵上清液对金黄色葡萄球菌具有极强的抑制作用,抑菌圈最高达(28.1±1.2)mm;3株菌对HT-29细胞均具有良好的黏附特性,其中WLRE01黏附能力最强。综上所述,健康家禽肠道源的3株产Reuterin的罗伊氏乳杆菌可作为益生菌候选菌株进行深入研究。 展开更多
关键词 罗伊氏乳杆菌 定向筛选 Reuterin 耐受性 抑菌 黏附
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Helicobacter pylori eradication: Sequential therapy and Lactobacillus reuteri supplementation 认领 引用 被引量：4
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作者 Cesare Efrati Giorgia Nicolini +2 位作者 Claudio Cannaviello Nicole Piazza O'Sed Stefano Valabrega 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第43期6250-6254,共5页
AIM:To evaluate the role of sequential therapy and Lactobacillus reuteri (L. reuteri ) supplementation, in the eradication treatment of Helicobacter pylori (H. pylori ). METHODS:H. pylori infection was diagnosed in 90... AIM:To evaluate the role of sequential therapy and Lactobacillus reuteri (L. reuteri ) supplementation, in the eradication treatment of Helicobacter pylori (H. pylori ). METHODS:H. pylori infection was diagnosed in 90 adult dyspeptic patients. Patients were excluded if previously treated for H. pylori infection or if they were taking a proton pump inhibitor (PPI), H2-receptor antagonist or antibiotics. Patients were assigned to receive one of the following therapies:(1) 7-d triple therapy (PPI plus clarithromycin and amoxicillin or metronidazole) plus L. reuteri supplementation dur- ing antibiotic treatment; (2) 7-d triple therapy plus L. reuteri supplementation after antibiotic treatment; (3) sequential regimen (5-d PPI plus amoxicillin therapy followed by a 5-d PPI, clarithromycin and tinidazole) plus L. reuteri supplementation during antibiotic treatment; and (4) sequential regimen plus L. reuteri supplementation after antibiotic treatment. Successful eradication therapy was defined as a negative urea breath test at least 4 wk following treatment. RESULTS:Ninety adult dyspeptic patients were en- rolled, and 83 (30 male, 53 female; mean age 57 ± 13 years) completed the study. Nineteen patients were administered a 7-d triple treatment:11 with L. reuteri supplementation during and 8 after therapy. Sixty-four patients were administered a sequential regimen:32 with L. reuteri supplementation during and 32 after therapy. The eradication rate was significantly higher in the sequential group compared with the 7-d triple regimen (88% vs 63%, P = 0.01). No difference was found between two types of PPI. No difference in erad- ication rates was observed between patients submitted to L. reuteri supplementation during or after antibiotic treatment. Compliance with therapy was excellent in all patients. No difference in adverse effects was observed between the different antibiotic treatments and between patients submitted to L. reuteri supplementation during and after antibiotic treatment. There was a low incidence of adverse effects in all groups of patients with sequential therapy, probably due to the presence of the L. reuteri supplementation. CONCLUSION:The sequential treatment regimen achieved a significantly higher eradication rate of H. pylori compared with standard 7-d regimen. L. reuteri supplementation could reduce the frequency and the intensity of antibiotic-associated side-effects. 展开更多
关键词 Helicobacter pylori Probiotics Lactobacillus reuteri Sequential therapy Gastritis Eradication
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Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri:intraspecific and interspecific patterns 认领 引用 被引量：3
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作者 Chunxiu Lin Yuxing Zheng +5 位作者 Bo Zhang Guopeng Lin Kexin Shang Jianxin Zhao Gang Wang Wei Chen 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第5期2799-2812,共14页
Lactobacillus rhamnosus(Rh)and Lactobacillus reuteri(Re)are well-known probiotic species in inflammatory bowel disease(IBD)research.The variations between these species’efficacy against colitis,and their model of act... Lactobacillus rhamnosus(Rh)and Lactobacillus reuteri(Re)are well-known probiotic species in inflammatory bowel disease(IBD)research.The variations between these species’efficacy against colitis,and their model of action in this regard,are intriguing and enable treatment to be individually tailored to patients.In this study,four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced.The anti-colitis activities of both strains involved various aspects of intestinal immune,physical,chemical,and biological barrier function.Strikingly,the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration.Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis.Nevertheless,Re strains were more effective than Rh strains in inhibiting production of inflammatory factors;promoting production of intestinal mucus,antimicrobial peptides,and tight junction proteins;and supporting the stem cell compartment.This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains.In addition,the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism-and prophage-related genes,respectively.Taken together,the results of this study could assist researchers in developing effective therapies for IBD. 展开更多
关键词 Inflammatory bowel disease Anti-colitis Lactobacillus rhamnosus Lactobacillus reuteri Intestinal barrier function Short-chain fatty acids
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Lactobacillus reuteri PYR8亚油酸异构酶基因在毕赤酵母中的表达 认领 引用 被引量：5
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作者 张艳禾 张兰威 +1 位作者 胡森 步志高 《浙江大学学报（农业与生命科学版）》 EI CAS 北大核心 2006年第5期505-510,共6页
以Lactobacillus reuteriPYR8菌株基因组DNA为模板,利用PCR方法扩增出亚油酸异构酶(linoleate isomerase,LI)基因,将其克隆至酵母表达载体pPIC9K中,电转化至毕赤酵母GS115中.重组菌株GS115/pPIC9K-LI经1%甲醇诱导后,SDS-PAGE电泳表明该... 以Lactobacillus reuteriPYR8菌株基因组DNA为模板,利用PCR方法扩增出亚油酸异构酶(linoleate isomerase,LI)基因,将其克隆至酵母表达载体pPIC9K中,电转化至毕赤酵母GS115中.重组菌株GS115/pPIC9K-LI经1%甲醇诱导后,SDS-PAGE电泳表明该基因在酵母中得到表达,即分子量约为67 kD的重组LI蛋白.经SP-Sepharose Fast Flow阳离子交换层析、Phenyl Sepharose Fast Flow疏水层析纯化获得了纯度为95%的重组LI蛋白样品.气相色谱分析表明,纯化的重组LI具有亚油酸异构酶的活性,酶的比活力为6.8 U?mg-1. 展开更多
关键词 巴斯德毕赤酵母 lactobacillus reuteri PYR8 亚油酸异构酶 共轭亚油酸 气相色谱
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以高嘌呤饮食评估罗伊氏乳杆菌Lactobacillus reuteri GKR1之降尿酸效果 认领 引用 被引量：1
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作者 王启宪 林诗伟 +2 位作者 赵敞 陈炎炼 陈劲初 《食品与营养科学》 CAS 2022年第1期56-63,共8页
近年来,高尿酸血症的盛行率居高不下,高嘌呤饮食为引起高尿酸血症之重要因素,高尿酸血症会增加罹患心血管疾病、糖尿病、肾脏病功能异常、高血压等疾病的机率,而降尿酸药物具有副作用,因此需开发替代治疗之策略。本试验先以体外试验筛... 近年来,高尿酸血症的盛行率居高不下,高嘌呤饮食为引起高尿酸血症之重要因素,高尿酸血症会增加罹患心血管疾病、糖尿病、肾脏病功能异常、高血压等疾病的机率,而降尿酸药物具有副作用,因此需开发替代治疗之策略。本试验先以体外试验筛选具有降尿酸潜力菌株,发现效果最佳菌株为Lactobacillus reuteri GKR1,再以高嘌呤饮食饲料评估Lactobacillus reuteri GKR1于小鼠之降尿酸效果,试验将ICR小鼠分为5组:正常对照组、负对照组、阳性对照组、低剂量GKR1组及高剂量GKR1组,连续14天将试验物质经管喂投予小鼠,在试验第7及第14天收集各组小鼠血清,分析尿酸(Uric acid)、尿素氮(BUN)与肌肝酸(Creatinine)含量,在试验第14天采集小鼠肝脏分析黄嘌呤氧化酶活性。实验结果显示,低、高剂量组GKR1可降低血清中尿酸、尿素氮含量,高剂量GKR1组显着减少肝脏中黄嘌呤氧化酶活性,综合以上结果,GKR1具有开发为降尿酸保健食品之潜力。 展开更多
关键词 Lactobacillus reuteri GKR1 益生菌 尿酸 黄嘌呤氧化酶 高嘌呤饮食
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Lactobacillus reuteri IMAU10240增殖培养基及高密度培养工艺优化 认领 引用 被引量：12
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作者 姚国强 张雪梅 +3 位作者 高志敏 赵燕霞 孙天松 张和平 《食品科学》 EI CAS CSCD 北大核心 2017年第14期97-105,共9页
Lactobacillus reuteri IMAU10240(L.reuteri IMAU10240)是一株具有潜在益生特性的乳酸菌,为实现产业化应用,对其培养工艺进行优化以提高其活菌数。本研究以MRS培养基为基础,通过单因素筛选试验、正交试验和响应面优化试验对该菌株的培... Lactobacillus reuteri IMAU10240(L.reuteri IMAU10240)是一株具有潜在益生特性的乳酸菌,为实现产业化应用,对其培养工艺进行优化以提高其活菌数。本研究以MRS培养基为基础,通过单因素筛选试验、正交试验和响应面优化试验对该菌株的培养基以及培养条件进行优化。通过实验确定L.reuteri IMAU10240最适培养基:蔗糖100.00 g/L,大豆蛋白胨13.25 g/L,酵母粉8.84 g/L,酵母蛋白胨13.25 g/L,Na_2HPO_4 19.85 g/L,柠檬酸2.58 g/L,MnSO_4·5H_2O 0.12 g/L,MgSO_4·7H_2O 0.40 g/L,甘油2.76 g/L,吐温-80 1.00 g/L,L-半胱氨酸盐酸盐0.50 g/L。初始pH 6.5,通入氮气37℃恒pH 5.5培养7~8 h。利用优化好的配方工艺进行5 L/50 L/200 L发酵罐的小试及中试试验,验证L.reuteri IMAU10240的发酵工艺,活菌数为7.57×10~9 CFU/mL,冻干菌粉活菌数为2.59×1011 CFU/g,可以进行生产验证。 展开更多
关键词 罗伊氏乳杆菌 增殖培养基 高密度培养
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