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The occurrence and genetic diversity of vegetable root-knot nematodes in Xinjiang Uygur Autonomous Region 认领 引用
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作者 Junhui Zhou Yuxuan Zhao +9 位作者 Wenfang Luo Hudie Shao Wei He Deliang Peng Wenkun Huang Huiqin Wang Honghai Zhao Jianjun Xu Huan Peng 无 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第6期2475-2484,共10页
Root-knot nematodes(RKNs)are the most economically damaging plant-parasitic nematodes globally.Xinjiang,encompassing one-sixth of China's landmass,currently lacks comprehensive data regarding the occurrence,distri... Root-knot nematodes(RKNs)are the most economically damaging plant-parasitic nematodes globally.Xinjiang,encompassing one-sixth of China's landmass,currently lacks comprehensive data regarding the occurrence,distribution,and genetic variation of RKNs infecting vegetables within its borders.Hence,identifying RKNs species and genetic diversity is crucial for devising comprehensive management strategies.Between 2021 and 2023,we present a survey of 130 samples,collected from 86 counties across 14 cities in Xinjiang,China,aiming to comprehensively understand the occurrence,distribution,damage,and species of vegetable RKNs.The results indicated that 57 out of 130 samples collected from the regions of Hami,Tulufan,Ili,Bayingol,Hotan,Aksu,Kashgar,and Kizilsu in Xinjiang were infected by RKNs,suggesting an expansion of RKN disease in the vegetable-producing regions of Xinjiang.The infected vegetable roots were found to harbor Meloidogyne incognita and M.hapla,with M.incognita being the most prevalent species.A phylogenetic analysis of the COI region revealed significant evolutionary divergence between M.incognita populations from Xinjiang and those from southeastern provinces.Haplotype analysis of the COI gene revealed that M.incognita populations are categorized into three major lineages:Asia,Europe,and a combined lineage encompassing both America and Africa.Notable gene fiow patterns were observed among M.incognita populations,with significant migrations from Europe and America to Asia,specifically from the southeastern China towards Xinjiang.This study's findings indicate a consistent increase in the detrimental effects of vegetables production caused by RKNs in Xinjiang.Implementing effective prevention and control measures is crucial to mitigate the spread of RKNs. 展开更多
关键词 root-knot nematode vegetables occurrence genetic diversity Xinjiang
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A tomato NBS-LRR gene Mi-9 confers heat-stable resistance to root-knot nematodes 认领 引用
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作者 Shudong Chen Yupan Zou +1 位作者 Xin Tong Cao Xu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2025年第7期2869-2875,共7页
Root-knot nematodes(RKNs)are the most widespread soil-borne obligate endoparasites.They can infect the roots of many crops and cause significant yield losses.The only commercially available RKN-resistant gene in tomat... Root-knot nematodes(RKNs)are the most widespread soil-borne obligate endoparasites.They can infect the roots of many crops and cause significant yield losses.The only commercially available RKN-resistant gene in tomatoes,Mi-1.2,fails at soil temperatures above 28℃.We cloned the heat-stable RKN-resistant gene,Mi-9,from a gene cluster composed of seven nucleotide-binding sites and leucine-rich repeat(NBS-LRR)type resistant genes in Solanum arcunum accession LA2157.Screening nematode infections in individual and combinatorial knockouts of five NBS-LRR genes showed that Mi-9 Candidate 4(MiC-4)alone is sufficient to confer heat-stable RKN resistance.Our study identifies a new source of heat-stable resistance to RKN in tomatoes for challenging environmental conditions.We also showcase a roadmap for rapid characterization of resistance genes by combining comparative genomics and genome editing,with the potential to be utilized in other crops. 展开更多
关键词 tomato nematode heat-stable resistance Mi-9 genome editing
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Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola 认领 引用 被引量：5
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作者 FENG Hui ZHOU Can-rong +5 位作者 ZHU Feng LE Xiu-hu JING De-dao Paul DALY ZHOU Dong-mei WEI Li-hui 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第10期3081-3089,共9页
Meloidogyne graminicola has emerged as one of the most destructive plant-parasitic nematodes affecting rice(Oryza sativa)production worldwide.Resistance to M.graminicola in rice could be the most effective option for ... Meloidogyne graminicola has emerged as one of the most destructive plant-parasitic nematodes affecting rice(Oryza sativa)production worldwide.Resistance to M.graminicola in rice could be the most effective option for its management.However,sources of germplasm with resistance to M.graminicola in rice remain limited.Here,we describe the root attraction,gall formation and genetic analysis of the resistance to M.graminicola in the rice variety Huidao 5.A nematode attraction assay showed that second-stage juveniles(J2s)of M.graminicola were attracted at the root tip of Huaidao 5 within 8 h without a significant reduction in attraction compared to the susceptible rice variety Nanjing 9108.Microscopic observation of the infection revealed that the J2s invaded root tissues 12 h after inoculation,but their subsequent movement to the root tip was hindered in Huaidao 5,resulting in decreased nematode number compared to Nanjing 9108.Additionally,we used the soil and hydroponic culture systems to simulate upland and flooding conditions in the paddy fields respectively,and found that gall number was significantly reduced,and nematode development was clearly suppressed in Huaidao 5.To investigate the genetic basis of this resistance,cross breeding was performed between the Huaidao 5 and Nanjing 9108 varieties.There was no reduction in the resistance of the F1 offspring to M.graminicola in the greenhouse or field trials,suggesting that a dominant gene could control resistance in Huaidao 5.In summary,this study provides a detailed characterization of a novel source of resistance to M.graminicola in rice,which is of great potential for use in crop breeding. 展开更多
关键词 root-knot nematode Meloidogyne graminicola rice resistance
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Study on the Activity of Herbal Extract to Kill Root-knot Nematode of Cucumber Seedlings Stage 认领 引用 被引量：4
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作者 ZHANG Min SU Kang-yu +3 位作者 LU Sheng MA Yong-qing DONG Shu-qi SONG Ji-qing 《Agricultural Science & Technology》 CAS 2010年第5期129-133,共5页
Present mature plants hydroponic technology was used,combined with some excellent characteristics,such as growth conditions was easy to control and process of root growth was easy to continuously observe,the nematicid... Present mature plants hydroponic technology was used,combined with some excellent characteristics,such as growth conditions was easy to control and process of root growth was easy to continuously observe,the nematicidal activity of 5 kinds of Chinese herbs extracts and the compound solution of Avermectin,with strong contact toxicity effect indoor,was systematically studied and investigated the affection on the root-knot nematode parasitized on the cucumber seeding stage.It is found that under the premise of no influence on root growth of cucumber,extracts from Picrorhiza scrophulariiflora and Punica granatum showed strong prevention and nematicidal activity,and had the similar efficacy of Avermectin;while the extracts from Cibotium barometz,Aucklandia lappa Decne and Fructus cnidii showed low nematicidal activity and various degrees inhibition effect on plant growth. 展开更多
关键词 Chinese herbal extract Cucumber Hydroponics Root-knot nematode Nemeticidal activity
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Investigation on Pathogenic Factors of Tobacco Root-Knot Nematode Disease in Gengma County,Yunnan Province 认领 引用 被引量：4
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作者 董莹 王晓强 +4 位作者 夏振远 谢桂花 王扬 谢勇 崔华栋 《Agricultural Science & Technology》 CAS 2014年第12期2253-2258,共6页
Tobacco root-knot nematode disease has caused severe damage in Geng- ma County, Yunnan Province. In order to identify the pathogenic factors of the tobac- co root-knot nematode disease in this county, the pathogenic n... Tobacco root-knot nematode disease has caused severe damage in Geng- ma County, Yunnan Province. In order to identify the pathogenic factors of the tobac- co root-knot nematode disease in this county, the pathogenic nematodes, hosts and environment of tobacco fields in Mengsa, Hepai and Sipaishan 3 main tobacco-grow- ing towns in Gengma County were investigated and analyzed based on the local re- lated field survey on tobacco root-knot nematode disease in this county in 2012. The results showed the incidence and severity of the tobacco root-knot nematode disease were all higher than those of previous years. dominant pathogens of the tobacco root-knot The species identification showed the nematode disease were Meloidogyne arenaria and M. javanica in Gengma County. The lacking of disease-resistant culti- vars, poor management and climatic anomaly were the main causes of the tobacco root-knot nematode disease in Gengma. According to the occurrence characteristics of the disease, the agricultural prevention-based control measures were proposed. 展开更多
关键词 Tobacco Root-knot nematode Identification of species Pathogenic factor
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Effects of Two Composted Plant Pesticide Residues,Incorporated with Trichoderma viride,on Root-Knot Nematode in Balloonflower 认领 引用 被引量：7
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作者 ZHANG Shuang-xi ZHANG Xing 《Agricultural Sciences in China》 2009年第4期447-454,共8页
Plant pesticide residues,such as chinaberry(Melia toosendan)residue and sand cypress(Sabina vulgaris)residue,are pesticidal plant materials discarded after the bioactive ingredient has been extracted with organic solv... Plant pesticide residues,such as chinaberry(Melia toosendan)residue and sand cypress(Sabina vulgaris)residue,are pesticidal plant materials discarded after the bioactive ingredient has been extracted with organic solvents.The only option for botanical pesticide residue utilization has been as landfill.Chinaberry residue(CBR)and sand cypress residue(SCR)were collected and composted in Yangling,Shaanxi Province,China.We studied the effects of chinaberry residue compost(CBRC),CBRC incorporated with Trichoderma viride(CBRCT),sand cypress residue compost(SCRC),and SCRC incorporated with T.viride(SCRCv)on the root-knot nematode,Meloidogyne incognita,infesting the balloonflower(Platycodon grandiflorum).Bioassay results indicated that stock solutions of the CBRCT and SCRCT extracts significantly inhibited egg hatching and caused high larval mortality,followed in degree by the CBRC and SCRC extracts.The CBR and SCR extracts caused very low inhibition of eggs and larvae.Supplementing potting mixtures with these four composts reduced the severity of root galling and increased the proportion of marketable roots.The severity of root galling decreased and the average weight of the marketable roots increased with an increase in all the composts when supplemented at rates from 5 to 30%.CBR-and SCR-supplemented pot soils also inhibited the nematodes,but CBR and SCR applied to the soil had a phytotoxic effect and inhibited balloonflower growth.Supplementing field soil with the composts reduced the severity of root galling and the populations of southern root-knot nematodes in the soil.CBRCT and SCRCT clearly enhanced the average weight of the marketable roots by 30.45 and 26.64%,respectively.Continuous supplementation with CBRCT or SCRCT in the same field significantly enhanced the control of the root-knot nematode,and the populations of nematodes continued to decrease with second inoculations.The populations of total Trichoderma spp.were distinctly enhanced and were maintained at high levels for a long time in the supplemented soils. 展开更多
关键词 balloon flower root-knot nematode plant pesticide residue compost compost extract
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Correction to:Local and systemic transcriptome and spliceome reprogramming induced by the root-knot nematode Meloidogyne incognita in tomato 认领 引用
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《Horticulture Research》 SCIE CSCD 2025年第3期390-390,共1页
This is a correction to:Selin Ozdemir et al.,Local and systemic transcriptome and spliceome reprogramming induced by the root-knot nematode Meloidogyne incognita in tomato,Horticulture Research,Volume 11,Issue 9,Septe... This is a correction to:Selin Ozdemir et al.,Local and systemic transcriptome and spliceome reprogramming induced by the root-knot nematode Meloidogyne incognita in tomato,Horticulture Research,Volume 11,Issue 9,September 2024,uhae206,http://gffzzd3cc09b8251d45dfs9xfo59f069o66wxc.ffgz.tsg.suse.edu.cn/10.1093/hr/uhae206.M.incognita was incorrectly expanded to read Megalaima incognita.This has been corrected to read Meloidogyne incognita.The Publisher apologizes for the error. 展开更多
关键词 transcriptome meloidogyne incognita tomato meloidogyne incognitathe root knot nematode spliceome reprogramming spliceome megalaima incognitathis
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Study on Controlling Tobacco Root-knot Nematode by Fertilizer and Pesticide Synergy 认领 引用 被引量：1
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作者 梁兵 黄坤 +5 位作者 李宏光 王全明 钟晓田 白章信 邵小东 胡承孝 《Agricultural Science & Technology》 CAS 2017年第11期2127-2131,共5页
Synergism between pesticide and fertilizer in controlling root-knot nematode was investigated by field randomized block test. The results showed that the best control efficiency was shared by 0.5% abamectin and 25% mi... Synergism between pesticide and fertilizer in controlling root-knot nematode was investigated by field randomized block test. The results showed that the best control efficiency was shared by 0.5% abamectin and 25% mixed pesticide （abamectin and carbosulfan）, 24.1% and 28.0%, respectively. The positive effect was found by synergism between pesticide and fertiUzer in controlling reot-knot nematode. The economic benefit and agricultural benefit were gained by controlling root-knot nematodes. The results suggested that the agricultural and ecological benefit could be gained through the integration of agricultural practice and chemical technology in controlling root-knot nematode. 展开更多
关键词 Root-knot nematode Pesticide Fertilizer Synergism Tobacco
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Effect of Anti-nematode Preparations on Physiological Traits of Tomato Leaves Affected by Root-knot Nematode 认领 引用
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作者 卢树昌 刘慧芹 +1 位作者 王小波 毕于慧 《Plant Diseases and Pests》 2011年第3期65-68,共4页
[ Objective ] The paper was to study the effects of anti-nematode preparations with different mechanisms on changes of enzyme systems and membrane permeability of tomato leaves, so as to provide reference basis for ef... [ Objective ] The paper was to study the effects of anti-nematode preparations with different mechanisms on changes of enzyme systems and membrane permeability of tomato leaves, so as to provide reference basis for effective control of soil root-knot nematode in greenhouse. [ Method] With tomato seedlings af- fected by root-knot nematode as material, changes of superoxide dismutase（SOD）, peroxidase（ POD）, relative conductivity and malondialdehyde （MDA） in toma- toes were tested after the seedling soil was treated by preparations of Wuxianmei, Hailvsu, Duxiandna and Avermectin. [ Result] After treated by different prepara- tions, SOD and POD activity of tomato leaves were higher than control, and that treated by Wuxianmei was the highest. In addition to Duxiandna, the relative con- ductivity and MDA content of other treatments were significantly lower than control. When tomatoes were planted for 70 d, the effect of Avermectin against reot-knot nematode Was the best of 66.3%. [ Conclusion] After tomatoes were infected by root-knot nematode, different preparation treatments all had certain control effect, which made the physical indicators of tomato have obvious change. Integrated control of multiple preparations in greenhouse was beneficial to control soil root-knot nematode. 展开更多
关键词 Tomato Root-knot nematode Preparation Leaf Physiological trait China
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Control of Southern Root-knot Nematodes on Tomato and Regulation of Soil Bacterial Community by Biofumigation with Zanthoxylum bungeanum Seed 认领 引用 被引量：8
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作者 Xiaoyun Wang Wencong Shi +4 位作者 Jilei Wang Zheng Gao Shuang Li Ningxin Wang Qinghua Shi 《Horticultural Plant Journal》 SCIE CAS CSCD 2021年第1期49-58,共10页
Biofumigation is an environmentally friendly strategy used to control nematodes and plant diseases.The volatile oil of Zanthoxylum bungeanum has high insecticidal and antibacterial activity.However,it is not known if ... Biofumigation is an environmentally friendly strategy used to control nematodes and plant diseases.The volatile oil of Zanthoxylum bungeanum has high insecticidal and antibacterial activity.However,it is not known if the seed of Z.bungeanum is a suitable material for biofumigation to control southern root-knot nematodes(SRKN)on tomato,and how it may regulate the soil bacterial community structure.We used pot experiments in the greenhouse to determine the effects of Z.bungeanum seeds on SRKN,plant growth parameters,soil physicochemical and microbial characteristics.A total of 26 volatile components,including nematicidally active substances,were identified from Z.bungeanum seeds.Z.bungeanum seed biofumigation significantly reduced the SRKN population by 88.89%and 81.55%on the 50th and 100th day after transplanting,respectively.Compared to the control,the total soluble sugar,soluble solids,soluble protein,titratable acid,root activity and the fruit yield per tomato plant increased significantly.The content of soil alkali-hydrolyzed nitrogen,available potassium and the soil enzyme activities were also significantly increased.The soil bacterial diversity and the co-occurrence network complexity were increased by Z.bungeanum seed biofumigation.Relativelymore keystone OTUs in biofumigation soil had potential plant growth-promoting capabilities.The function of Z.bungeanum seed increasing tomato production in SRKN-infected soil depends on directly killing SRKN and improving soil properties.These results indicate that Z.bungeanum seed can be used as both a nematicide and a high quality organic fertilizer in tomato production. 展开更多
关键词 biofumigant Zanthoxylum bungeanum seed root-knot nematode tomato soil bacterial community
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Preparation and efficacy evaluation of Paenibacillus polymyxa KM2501-1 microbial organic fertilizer against root-knot nematodes 认领 引用 被引量：5
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作者 CHENG Wan-li ZENG Li +7 位作者 YANG Xue HUANG Dian YU Hao CHEN Wen CAI Min-min ZHENG Long-yu YU Zi-niu ZHANG Ji-bin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第2期542-551,共10页
Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these t... Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these two problems, this study developed a procedure for the conversion of chicken manure to organic fertilizer by larvae of Hermetia illucens L. and Bacillus subtilis BSF-CL. Chicken manure organic fertilizer was then mixed thoroughly with Paenibacillus polymyxa KM2501-1 to a final concentration of 1.5×108CFU g-1. The efficacy of KM2501-1 microbial organic fertilizer in controlling root-knot nematodes was evaluated in pot and field experiments. In pot experiments, applying KM2501-1 microbial organic fertilizer either as a base fertilizer or as a fumigant at the dose of 40 g/pot suppressed root-knot disease by 61.76 and 69.05% compared to the corresponding control treatments, respectively. When applied as a fumigant at the dose of 1 kg m;in field experiments, KM2501-1 microbial organic fertilizer enhanced the growth of tomato plants, suppressed root-knot disease by 49.97%, and reduced second stage juveniles of RKN in soil by 88.68%. KM2501-1 microbial organic fertilizer controlled RKNs better than commercial bio-organic fertilizer in both pot and field experiments. These results demonstrate that this co-conversion process efficiently transforms chicken manure into high value-added larvae biomass and KM2501-1 microbial organic fertilizer with potential application as a novel nematode control agent. 展开更多
关键词 chicken manure Hermetia illucens L. microbial organic fertilizer root-knot nematode field experiment
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Morphology and Molecular Identification of Root-knot Nematode in Partial Tobacco-growing Areas in Yunnan Province 认领 引用
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作者 Yang Yanmei Liang Yan +2 位作者 Yuan Shaojie Zhao Xicheng Hu Xianqi 《Plant Diseases and Pests》 2017年第2期13-19,共7页
[Objectives]The species and distribution of root-knot nematodes in tobacco growing regions of Yunnan were investigated,in order to provide reference for comprehensive prevention of root-knot nematodes disease and toba... [Objectives]The species and distribution of root-knot nematodes in tobacco growing regions of Yunnan were investigated,in order to provide reference for comprehensive prevention of root-knot nematodes disease and tobacco variety layout. [Methods]Fifty-four samples of root-knot nematode host on tobacco crops were collected from Kunming,Qujing and Yuxi cities in Yunnan from August to October in 2015. The species of root-knot nematodes were identified by observing perineal patterns and molecular biological testing（ PCR of ribosomal DNA of ITS region and sequence characterized amplified region,SCAR）. [Results] There were 24 samples belonging to Meloidogyne arenaria,accounting for 44. 44%; seven M. incognita samples,accounting for 12. 96%; one M. javanica sample,accounting for 1. 85%. Eleven samples were compound populations of M. javanica and M. arenaria,accounting for 20. 37%. Five were compound populations of M. incognita and M. arenaria,accounting for 9. 26%. Three were compound populations of M. incognita and M. javanica,accounting for 5. 56%. Three belonged to compound population of M. incognita,M. javanica and M. arenaria,accounting for 5. 56%. [Conclusions] The species of root-knot nematodes in tobacco growing areas of Yunnan mainly include M. incognita,M. javanica and M. arenaria. M. arenaria is the dominant population. 展开更多
关键词 Tobacco Root-knot nematode Species Yunnan
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The synergistic advantage of combining chloropicrin or dazomet with fosthiazate nematicide to control root-knot nematode in cucumber production 认领 引用 被引量：11
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作者 HUANG Bin WANG Qian +7 位作者 GUO Mei-xia FANG Wen-sheng WANG Xiao-ning WANG Qiu-xia YAN Dong-dong OUYANG Can-bin LI Yuan CAO Ao-cheng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第9期2093-2106,共14页
The highly-damaging root-knot nematode(Meloidogyne spp.,RKN)cannot be reliably controlled using only a nematicide such as fosthiazate because of increasing pest resistance.In laboratory and greenhouse trials,we showed... The highly-damaging root-knot nematode(Meloidogyne spp.,RKN)cannot be reliably controlled using only a nematicide such as fosthiazate because of increasing pest resistance.In laboratory and greenhouse trials,we showed that chloropicrin(CP)or dazomet(DZ)synergized the efficacy of fosthiazate against RKN.The combination significantly extended the degradation half-life of fosthiazate by an average of about 1.25 times.CP or DZ with fosthiazate reduced the time for fosthiazate to penetrate the RKN cuticle compared to fosthiazate alone.CP or DZ combined with low or medium rate of fosthiazate increased the total cucumber yield,compared to the use of each product alone.A low-dose fosthiazate with DZ improved total yield more than a low dose fosthiazate with CP.Extending the half-life of fosthiazate and reducing the time for fosthiazate or fumigant to penetrate the RKN cuticle were the two features that gave the fumigant-fosthiazate combination its synergistic advantage over these products used singularly.This synergy provides the opportunity for farmers to use a low dose of fosthiazate which lowers the risk of RKN resistance.Farmers could combine DZ at 30 g m^-2 with fosthiazate at a low rate of 0.375 g m^-2 to control RKN and adequately control two major soil-borne diseases in cucumber greenhouses. 展开更多
关键词 dazomet chloropicrin fosthiazate cucumber root-knot nematode
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Bacterial artificial chromosome library construction of root-knot nematode resistant pepper genotype HDA149 and identification of clones linked to Me3 resistant locus 认领 引用
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作者 GUO Xiao YANG Xiao-hui +5 位作者 YANG Yu MAO Zhen-chuan LIU Feng MA Wei-qing XIE Bing-yan LI Guang-cun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第1期57-64,共8页
Pepper （Capsicum annuum. L.） is a widely cultivated vegetable crop worldwide and has the second largest planting area and the first largest vegetable output and value in China. Pepper root-knot nematode （Meloidogyn... Pepper （Capsicum annuum. L.） is a widely cultivated vegetable crop worldwide and has the second largest planting area and the first largest vegetable output and value in China. Pepper root-knot nematode （Meloidogyne spp.） is one of the most serious pests of pepper, which caused huge losses every year. Previous studies showed that the Me3 gene is resistant to a wide range of Meloidogyne species, including M. arenaria, M. javanica, and M. incognita. HDA149, a double haploid pepper genotype, harboring the root-knot nematode resistance gene Me3, was used to construct bacterial artificial chro- mosome library （BAC） via the vector of CopyControFM pCC1 in this study. The library consists of 210 200 BAC clones and is equivalent to 5.3 pepper genomes. The average insert size is 95 kb, and most of them are 90-120 kb; but the empty clones are less than 3%. In order to screen the BAC library easily, 550 super pools with 384 BAC clones of each pool were further developed in this study. Specific primers from Me3 gene locus were used for BAC library screening, and more than 20 positive BAC clones were obtained. Then the selected positive BAC clones were analyzed by restriction enzyme digestion, BAC-end sequencing, marker development, and new positive BAC clones exploration, respectively. Finally, the contig with total length of about 300 kb linked to the Me3 locus was constructed based on chromosome walking strategy, which made a solid foundation for the cloning of the important root-knot nematode resistance gene Me3. 展开更多
关键词 pepper bacterial artificial chromosome library.（BAC） root-knot nematode Me3 gene contig
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Toxicity Test of Different Pesticides on Tobacco Root-knot Nematodes 认领 引用 被引量：2
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作者 Fei DAI Peibo LI +1 位作者 Quan ZHANG Enfa CHEN 《Agricultural Biotechnology》 2020年第5期86-89,共4页
[Objectives]This study was conducted to quickly screen out effective and safe agents for controlling tobacco root knot nematode disease.[Methods]Six pesticides were tested and screened indoors at five different concen... [Objectives]This study was conducted to quickly screen out effective and safe agents for controlling tobacco root knot nematode disease.[Methods]Six pesticides were tested and screened indoors at five different concentrations.[Results]The six pesticides all had toxic effects on the second-instar larvae of tobacco root-knot nematode,and the corrected mortality was positively correlated with the concentration and time of the pesticides,with the correlation coefficients above 0.8.From the perspective of inhibitory activity,the order was fosthiazate>abamectin>emamectin benzoate>cadusafos>ethoprophos>carbosulfan.Four pesticides that can be used in the field were selected.[Conclusions]This study provides a theoretical basis for field pesticide selection. 展开更多
关键词 Tobacco Root-knot nematode Medicament Virulence determination Screening
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SlWRKY45 interacts with jasmonate-ZIM domain proteins to negatively regulate defense against the root-knot nematode Meloidogyne incognita in tomato 认领 引用 被引量：12
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作者 Huang Huang Wenchao Zhao +7 位作者 Hui Qiao Chonghua Li Lulu Sun Rui Yang Xuechun Ma Jilin Ma Susheng Song Shaohui Wang 《Horticulture Research》 SCIE CSCD 2022年第1期3630-3642,共13页
Parasitic root-knot nematodes(RKNs)cause a severe reduction in crop yield and seriously threaten agricultural production.The phytohormones jasmonates(JAs)are important signals regulating resistance to multiple biotic ... Parasitic root-knot nematodes(RKNs)cause a severe reduction in crop yield and seriously threaten agricultural production.The phytohormones jasmonates(JAs)are important signals regulating resistance to multiple biotic and abiotic stresses.However,the molecular mechanism for JAs-regulated defense against RKNs in tomato remains largely unclear.In this study,we found that the transcription factor SlWRKY45 interacted with most JA-ZIM domain family proteins(JAZs),key repressors of the JA signaling.After infection by the RKN Meloidogyne incognita,the slwrky45 mutants exhibited lower gall numbers and egg numbers per gram of roots than wild type,whereas overexpression of SlWRKY45 attenuated resistance to Meloidogyne incognita.Under M.incognita infection,the contents of jasmonic acid(JA)and JA-isoleucine(JA-Ile)in roots were repressed by SlWRKY45-overexpression.Furthermore,SlWRKY45 bound to and inhibited the promoter of the JA biosynthesis gene ALLENE OXIDE CYCLASE(AOC),and repressed its expression.Overall,our findings revealed that the SlJAZ-interaction protein SlWRKY45 attenuated RKN-regulated JA biosynthesis and repressed defense against the RKN M.incognita in tomato. 展开更多
关键词 jasmonates tomato jasmonate zim domain proteins root knot nematode phytohormones jasmonates jas meloidogyne incognita defense slwrky
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Evaluation of the biocontrol potential of Aspergillus welwitschiae against the root-knot nematode Meloidogyne graminicola in rice(Oryza sativa L.) 认领 引用 被引量：3
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作者 LIU Ying DING Zhong +6 位作者 PENG De-liang LIU Shi-ming KONG Ling-an PENG Huan XIANG Chao LI Zhong-cai HUANG Wen-kun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第11期2561-2570,共10页
The root-knot nematode Meloidogyne graminicola is considered one of the most devastating pests in rice-producing areas,and nematicides are neither ecofriendly nor cost effective.More acceptable biological agents are r... The root-knot nematode Meloidogyne graminicola is considered one of the most devastating pests in rice-producing areas,and nematicides are neither ecofriendly nor cost effective.More acceptable biological agents are required for controlling this destructive pathogen.In this study,the biocontrol potential of Aspergillus welwitschiae AW2017 was investigated in laboratory and greenhouse experiments.The in vitro ovicidal and larvicidal activities of A.welwitschiae metabolites were tested on M.graminicola in laboratory experiments.The effect of A.welwitschiae on the attraction of M.graminicola to rice and the infection of rice by M.graminicola was evaluated in a greenhouse.The bioagent AW2017 displayed good nematicidal potential via its ovicidal and larvicidal action.The best larvicidal activity was observed at a concentration of 5×AW2017,which caused an 86.2%mortality rate at 48 h post inoculation.The highest ovicidal activity was recorded at a concentration of 5×AW2017,which resulted in an approximately 47.3%reduction in egg hatching after 8 d compared to the control.Under greenhouse conditions,the application of A.welwitschiae significantly reduced the root galls and nematodes in rice roots compared to the control.At a concentration of 5×AW2017,juveniles and root galls in rice roots at 14 d post inoculation(dpi)were reduced by 24.5 and 40.5%,respectively.In addition,the attraction of M.graminicola to rice roots was significantly decreased in the AW2017 treatment,and the development of nematodes in the AW2017-treated plants was slightly delayed compared with that in the PDB-treated control plants.The results indicate that A.welwitschiae is a potential biological control agent against M.graminicola in rice. 展开更多
关键词 Meloidogyne graminicola Aspergillus welwitschiae biological fungus nematode development nematicidal potential
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Efficiency of Some Plant Essential Oils on Root-Knot Nematode Meloidogyne incognita 认领 引用 被引量：2
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作者 Esengul Ozdemir Ugur Gozel 《Journal of Agricultural Science and Technology(A)》 2017年第3期178-183,共6页
During the last few decades, researchers have been in the search for environmental friendly ways to cope with agricultural pests, instead of using chemical pesticides. The use of essential oils has a high potential to... During the last few decades, researchers have been in the search for environmental friendly ways to cope with agricultural pests, instead of using chemical pesticides. The use of essential oils has a high potential to become an alternative control strategy against plant parasitic nematodes. This study was conducted to determine the toxicity of 10 essential oils （Artemisia absinthium, Citrus bergamia, Eucalyptus citriodora, Hypericum perforatum, Lavandula officinalis, Mentha arvensis, Ocimum basilicum, Piper nigrum, Thymus serpyllum and Zingiber officinale） against the second stage juveniles of the root-knot nematode Meloidogyne incognita. The aqueous solutions of these essential oils have been applied to the second stage juveniles in three different concentrations （1%, 3% and 5%） at four different application time intervals （12, 24, 48 and 72 h）. The interactions between the variables have been examined with repeated measure analysis of variance （ANOVA）. This showed that the interactions of essential oil-time and essential oil-concentration were statistically significant, and it is concluded that L. officinalis, A. absinthium, P. nigrum, C. bergamia and M. arvensis have the most toxic effect in all concentrations and times, respectively. 展开更多
关键词 Root-knot nematode Meloidogyne incognita essential oils environmental friendly pest management.
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Negative regulation of root-knot nematode parasitic behavior by root-derived volatiles of wild relatives of Cucumis metuliferus CM3 认领 引用 被引量：3
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作者 Xiaoxiao Xie Jian Ling +7 位作者 Zhenchuan Mao Yan Li Jianlong Zhao Yuhong Yang Yanlin Li Mingyue Liu Xingfang Gu Bingyan Xie 《Horticulture Research》 SCIE CSCD 2022年第1期1533-1542,共10页
Root-knot nematodes(RKN;Meloidogyne spp.)cause a significant decrease in the yield of cucumber crops every year.Cucumis metuliferus is an important wild germplasm that has resistance to RKN in which plant root volatil... Root-knot nematodes(RKN;Meloidogyne spp.)cause a significant decrease in the yield of cucumber crops every year.Cucumis metuliferus is an important wild germplasm that has resistance to RKN in which plant root volatiles are thought to play a role.However,the underlying molecular mechanism is unclear.To investigate it,we used the resistant C.metuliferus line CM3 and the susceptible cucumber line Xintaimici(XTMC).CM3 roots repelled Meloidogyne incognita second-stage larvae(J2s),while the roots of XTMC plants attracted the larvae.CM3 and XTMC were found to contain similar amounts of root volatiles,but many volatiles,including nine hydrocarbons,three alcohols,two aldehydes,two ketones,one ester,and one phenol,were only detected in CM3 roots.It was found that one of these,(methoxymethyl)-benzene,could repel M.incognita,while creosol and(Z)-2-penten-1-ol could attract M.incognita.Interestingly,creosol and(Z)-2-penten-1-ol effectively killed M.incognita at high concentrations.Furthermore,we found that a mixture of CM3 root volatiles increased cucumber resistance to M.incognita.The results provide insights into the interaction between the host and plant-parasitic nematodes in the soil,with some compounds possibly acting as nematode biofumigation,which can be used to manage nematodes. 展开更多
关键词 negative regulation meloidogyne spp plant root volatiles cucumis metuliferus cucumber line root knot nematode root derived volatiles cucumber crops
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Determination of Nematicidal Effects of Some Biopesticides against Root-Knot Nematode (Meloidogyne incognita) on Kiwifruit 认领 引用 被引量：1
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作者 Ilker Kepenekci Onur Dura Sami Dura 《Journal of Agricultural Science and Technology(A)》 2017年第8期546-551,共6页
The aim of this study was to determine the nematicidal effects of some biopesticides againist root-knot nematodes （RKNs） （Meloidogyne incognita） on Bruno type kiwifruit seedlings. Research was conducted in the Ata... The aim of this study was to determine the nematicidal effects of some biopesticides againist root-knot nematodes （RKNs） （Meloidogyne incognita） on Bruno type kiwifruit seedlings. Research was conducted in the Atattirk Central Horticultural Research Institute （ACHRI, Yalova, Turkey） greenhouse during 2013-2014. Biopesticides observed in this study were： sesame oil （Devalone EC）, castor oil （Ricinus communis）, Myrothecium verrucaria （M. verrucaria strain AARC-0255, Inferno DF）, Glomus spp. （Endomycorrhizal fungi, Endo Roots Soluble SP） and Paecilomyces lilacinus （Entomopathogenic fungi, P. lilacinus strain PL1, Bionematon SL）. Nematicide Nemacur EC 400 （fenamiphos） was used as treated control （TC） and trials were set up as randomized parcel design （RPD） with seven characters and 10 replicates. Reproduction ratio was determined according to scale values in trial applications and second stage juvenile （J2） populations of M. incognita in soil. The results showed that the best nematicidal effects against RKNs were determined in castor oil （77.92% in 2013 and 73.61% in 2014） and P. lilacinus （61.03% in 2013 and 55.55% in 2014）. 展开更多
关键词 Root-knot nematodes Meloidogyne incognita sesame oil castor oil Myrothecium verrucaria Glomus Paecilomyceslilacinus kiwifruit.
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