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Transcriptional regulation and hormone action analysis in the regeneration process of Zoysia japonica after mowing 认领 引用 被引量:1
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作者 Luyu Wang Kemeng Xiao +3 位作者 Yue Li Zhilin Zi Yan Sun Qiannan Hu 《Grass Research》 2024年第1期89-102,共14页
Zoysia grass(Zoysia japonica)is an important native grass species in China,while the slow regeneration speed limits its use.Two Z.japonica materials FM1(fast regeneration rate)and SM232(slow regeneration rate)were cho... Zoysia grass(Zoysia japonica)is an important native grass species in China,while the slow regeneration speed limits its use.Two Z.japonica materials FM1(fast regeneration rate)and SM232(slow regeneration rate)were chosen from 10 different Zoysia grass by phenotypic index in this study.The genetic differences associated with zoysia regeneration and metabolism pathway are elucidated based on the transcriptome analysis,determination of hormone,and molecular biological section.Transcriptome sequencing results showed that both FM1 and SM232 had the maximum number of DEGs at 2 h and 12 h after mowing,indicating it may be the key stage of Zoysia grass response to mowing.After mowing treatment,FM1 was accompanied by up-regulation of regeneration-related genes,such as the LOB protein family and amide synthesis family.However,SM232 showed a continuous down-regulation of protein-related genes of the LOB family.Transcriptome analysis showed that the expression of IAA-related genes in both groups showed a downward trend,which was consistent with the results of IAA content determination.The IAA content in FM1 was significantly higher than that in SM232.Changes in CTK-related genes were only detected in FM1.Mowing induces plant defense systems,and plant tissue regeneration is closely related to the plant defense system,which may be the focus of further research.These results provide a basis for further research on the molecular mechanism of grass regeneration. 展开更多
关键词 zoysia japonica hormone action phenotypic index genetic differences transcriptional regulation zoysia grass molecular biological sectiontranscriptome sequencing transcriptome analysisdetermination
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