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The study on the effects of Paeoniae Radix Rubra-Angelicae Sinensis Radix before and after compatibility on energy metabolism in cold and heat syndrome model rats 认领 引用 被引量：1
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作者 Shi-Hao Zhang Jing Sun +6 位作者 Xiao-Fei Zhang Fei Luan Dong-Yan Guo Ying Wang Jun-Bo Zou Bing-Tao Zhai Jiang-Xue Cheng 《Traditional Medicine Research》 2026年第5期27-39,共13页
Background:Disorders of metabolism can affect the food intake,weight changes,and behavioral alterations of the body.Metabolic disorders are usually accompanied by the occurrence of diseases.We aimed to study the effec... Background:Disorders of metabolism can affect the food intake,weight changes,and behavioral alterations of the body.Metabolic disorders are usually accompanied by the occurrence of diseases.We aimed to study the effects of the compatibility of Paeoniae Radix Rubra(PRR)and Angelicae Sinensis Radix(ASR)on the metabolic level of rats,and observe the changes in body weight and behavior.Discover the mechanism of preventing the occurrence of diseases by using PRR and ASR.Methods:Two animal models were induced by levothyroxine and low-temperature stimulation,followed by 21 days of edible traditional Chinese medicine administration.The changes in the rats’water intake,food intake,body temperature,and thermotactic behavior were recorded.Results:The results showed that PRR could cause an increase in the body weight of rats,a decrease in body temperature,and a stronger preference for warm environments.PRR inhibited thyroid function,the excitability of the nervous system,and energy metabolism.PRR upregulated the expressions of mTOR and TRPM8 while downregulating the expressions of AMPK and TRPV1.Conclusion:Our research findings suggest that the cold-natured PRR can inhibit the material and energy metabolism of the body and lower the body temperature,increasing the thermophilic behavior of rats.In contrast,ASR exhibited an antagonistic effect against PRR. 展开更多
关键词 Paeoniae Radix Rubra Angelicae Sinensis Radix thermotropic behavior material metabolism energy metabolism
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Live Feed Drives the Succession of Bacterioplankton Community During Larval Breeding of Chinese Mitten Crab(Eriocheir sinensis)in Open Earthen Ponds 认领 引用
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作者 ZHAO Lianwen CHU Chang +4 位作者 STOECK Thorsten LU Haohao JIANG Jiamei WU Xugan PAN Hongbo 《Journal of Ocean University of China》 SCIE CAS CSCD 2026年第1期343-352,共10页
Open earthen pond-based larviculture is the main method for rearing Chinese mitten crab(Eriocheir sinensis)larvae in China.Bacterioplankton play a vital role in aquaculture ecosystems,but their community succession th... Open earthen pond-based larviculture is the main method for rearing Chinese mitten crab(Eriocheir sinensis)larvae in China.Bacterioplankton play a vital role in aquaculture ecosystems,but their community succession throughout the larval nursery stages of E.sinensis in such systems is still poorly understood.Using 16S rRNA gene amplicon sequencing,this study characterized the dynamics of bacterioplankton communities during a complete E.sinensis larval rearing cycle in open earthen ponds.Results presented a distinct,unidirectional succession in bacterioplankton composition,characterized by a decrease in Alphaproteobacteria and an increase in Actinobacteria.Live feeds(Brachionus plicatilis and Artemia salina)significantly affected bacterioplankton communities directly through their biological activities and indirectly by modifying the structure of phytoplankton composition.In addition to live feeds,water temperature and dissolved oxygen were key environmental factors affecting the structure of bacterioplankton communities.Biotic interactions,specifically with phytoplankton and copepods,were key drivers of bacterioplankton succession.Routine feeding practices during larval cultivation decreased bacterioplankton network complexity and strengthened community stability.Keystone taxa,specifically OTU783(Rhodobacteraceae)and OTU1235(Microbacteriaceae),played an essential role in maintaining the community stability through recruiting more rare species during the later stages despite their low abundances.These outcomes advance our understanding of the mechanisms central to bacterioplankton community succession and stability in E.sinensis larviculture systems,offering critical insights for developing sustainable aquaculture management strategies. 展开更多
关键词 Eriocheir sinensis larval nursery earthen ponds high-throughput sequencing bacterioplankton community network analysis
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Spatiotemporal Habitat Use in Juvenile Chinese Alligators(Alligator sinensis):Interactive Effects of Sunlight and Time 认领 引用
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作者 Jianjun MIAO Ke SUN +9 位作者 Shunli YE Yongkang ZHOU Zhenpeng YU Lei LI Pingsi YI Tongsheng XIA Genjun TU Dallin B.KOHLER Xiaobing WU Tao PAN 《Asian Herpetological Research》 SCIE CAS CSCD 2026年第2期157-165,共9页
The fine-scale habitat selection behavior of juvenile Chinese alligators(Alligator sinensis)is crucial to understand for ex situ environment design and in situ habitat restoration,yet how thermal requirements shape th... The fine-scale habitat selection behavior of juvenile Chinese alligators(Alligator sinensis)is crucial to understand for ex situ environment design and in situ habitat restoration,yet how thermal requirements shape their microhabitat use remains unclear.To address this,we studied ten juveniles in a semi-natural artificial pond with four environmentally distinct quadrants during September 2025.Using 24-h video surveillance and systematic sampling,we analyzed their spatial use patterns,diel rhythms,and environmental drivers via temporal trend analysis and generalized linear mixed modeling(GLMM).Results indicated heterogeneous space use across quadrants and spatially distinct diel rhythms.Habitat selection was best explained by an interaction between solar exposure ratio and time of day.The GLMM revealed a significant interaction between solar exposure ratio and time period(P<0.05),with simple slope analysis showing that solar exposure ratio had a strong positive effect on habitat use in the afternoon and night but not the morning.Concealment area also had a consistent positive effect.These findings demonstrate that juvenile Chinese alligators employ a dynamic,thermalrequirement-driven spatiotemporal strategy.This behavioral plasticity is critical for maintaining physiological homeostasis,suggesting that access to thermally heterogeneous habitats may be an important consideration in conservation planning for this species. 展开更多
关键词 Alligator sinensis behavioral thermoregulation habitat selection spatiotemporal patterns generalized linear mixed model conservation management
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Ophiocordyceps sinensis:decoding its journey—from snowy legends to modern science 认领 引用
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作者 Guiyu Li Ziwei Chen +6 位作者 Weiwei Tian Yang Liu Siye Yang Li Liu Ping Wei Junning Zhao Hua Hua 《Acupuncture and Herbal Medicine》 CAS CSCD 2026年第2期261-292,共32页
Ophiocordyceps sinensis(Berk)Sacc.(O.sinensis;Family:Clavicipitaceae;synonym:Cordyceps sinensis)is a fungus that is revered in ancient Tibetan folklore as a“miraculous species that becomes a worm in winter and a gras... Ophiocordyceps sinensis(Berk)Sacc.(O.sinensis;Family:Clavicipitaceae;synonym:Cordyceps sinensis)is a fungus that is revered in ancient Tibetan folklore as a“miraculous species that becomes a worm in winter and a grass in summer”and renowned as the“elixir of life gifted”by the snow-capped region.Its evolutional awareness and utilization span both traditional Chinese medicine practices and modern biomedical research,rendering it a quintessential example of symbiotic entomo-fungal biological resource development.Extensive research on the biological characteristics,active constituents,pharmacological effects,and underlying mechanisms of O.sinensis has generated significant breakthroughs in recent years.From“heavenly elixir”to a vital tonic in imperial cuisine,and from its symbiotic life cycle to its current prominence in modern pharmacological research and clinical applications,this fungus has nurtured both the cultural legends and survival wisdom of the Plateau ancestors and reflected the long journey of contemporary scientific exploration and industrialization.This review traces the journey of O.sinensis from traditional empirical applications to scientific validation,focusing on its biological characteristics,extraction and separation techniques for key bioactive constituents,and its pharmacological effects and molecular mechanisms in antitumor activity,immunomodulation,renoprotection,antioxidant properties,and anti-aging effects,and summarizes the current status of its clinical applications.Furthermore,this review examines the challenges confronting current research on O.sinensis,including resource conservation and sustainable utilization,quality control and standardization,differences between artificially cultivated and wild-harvested specimens,further elucidating its mechanisms of action and outlining prospects for future research directions. 展开更多
关键词 Active constituents Clinical applications Extraction and separation techniques Ophiocordyceps sinensis Pharmacological effects
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Study on the Mechanism of the Herb Pair Astragali Radix-Angelicae Sinensis Radix Against Cerebral Ischemia-Reperfusion Injury 认领 引用
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作者 Yanbin Li Chunhui Liu Hanrui Bai 《Journal of Clinical and Nursing Research》 2026年第5期372-377,共6页
Cerebral ischemia is a common acute cerebrovascular disease characterized by transient insufficient cerebral blood supply,manifesting as transient ischemic attacks.It has a high incidence,mortality,and disability rate... Cerebral ischemia is a common acute cerebrovascular disease characterized by transient insufficient cerebral blood supply,manifesting as transient ischemic attacks.It has a high incidence,mortality,and disability rate,and seriously affects human health and quality of life.The pathogenesis is complex and diverse,mainly involving inflammatory response,oxidative stress,and excitatory amino acid toxicity.As traditional blood-tonifying and strengthening herbs,Astragali Radix(Huangqi)and Angelicae Sinensis Radix(Danggui)not only exert favorable effects in treating cerebral ischemia,such as alleviating neurological damage,resisting inflammation,preventing thrombosis,and inhibiting cell death,but also achieve remarkable therapeutic efficacy.Furthermore,the two herbs can be used in combination to form the classic prescription Danggui Buxue Tang.This paper reviews how Astragali Radix,Angelicae Sinensis Radix,and their active ingredients synergistically exert anti-hypoxic effects in the brain. 展开更多
关键词 Yiqi Huoxue herb pair Astragali Radix Angelicae Sinensis Radix Cerebral ischemia Mechanism of action
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Aluminum-activated malate transporter family member CsALMT6 mediates fluoride resistance in tea plants(Camellia sinensis) 认领 引用 被引量：1
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作者 Qinghui Li Ruiming Zhang +3 位作者 Xinlong Hu Dejiang Ni Yuqiong Chen Mingle Wang 《Horticulture Research》 SCIE CSCD 2025年第4期16-28,共13页
Tea plant is a fluoride(F)-hyperaccumulator,which poses a potential threat to human health via tea consumption.Reducing F accumulation in fresh tea leaves is crucial for enhancing the safety of tea production at its s... Tea plant is a fluoride(F)-hyperaccumulator,which poses a potential threat to human health via tea consumption.Reducing F accumulation in fresh tea leaves is crucial for enhancing the safety of tea production at its source.This study aims to isolate novel genes responsible for F accumulation or transport in tea plants.We identified an aluminum(Al)-activated malate transporter gene,CsALMT6,which was hypothesized to be a candidate for differential F accumulation in Camellia sinensis,by employing a combination of transcriptome-wide association study and genome-wide identification of the CsALMT gene family.CsALMT6 exhibited high expression levels in old leaves,and its expression was significantly upregulated in tea plants subjected to F-stress conditions.Furthermore,heterologous expression of CsALMT6 in yeast,Arabidopsis,and Populus conferred F tolerance.However,the expression of F-tolerant hub genes,CsFEX1 and CsFEX2,remained unaffected in CsALMT6-silenced tea plants.Additionally,under F toxicity conditions,the transcription of CsALMT6 was negatively associated with F accumulation in tea plants.In conclusion,CsALMT6 plays a vital role in reducing F accumulation in C.sinensis,thus conferring F tolerance to plant cells. 展开更多
关键词 combination o enhancing safety tea production camellia sinensisby Camellia sinensis aluminum activated malate transporter tea plantswe tea plants fluoride resistance
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Evolutionary dynamics and functional characterization of Jasmonate ZIM-domain(JAZ)genes across Camellia sinensis pan-genome 认领 引用
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作者 Biao Xiong Yongyi Yang +1 位作者 Qingqing Li Suzhen Niu 《Beverage Plant Research》 CAS 2025年第1期43-50,共8页
Jasmonate ZIM-domain(JAZ)proteins regulate critical processes in plants,including growth,development,secondary metabolism,and responses to biotic and abiotic stresses.Previous studies primarily focused on single refer... Jasmonate ZIM-domain(JAZ)proteins regulate critical processes in plants,including growth,development,secondary metabolism,and responses to biotic and abiotic stresses.Previous studies primarily focused on single reference genomes,neglecting gene presence-absence variations(PAV)across populations.Investigating the JAZ gene family at the pan-genomic scale is thus essential to fully understand its evolutionary and functional dynamics in tea plants.Here,22 high-quality Camellia sinensis genomes were analyzed,and 21 JAZs exhibiting substantial presence-absence variability were identified.These included two core genes(present in all 22 genomes),three near-core genes(present in 20-21 genomes),ten dispensable genes(present in 2-19 genomes),and six private genes(unique to single genomes).Phylogenetic analysis categorized these JAZs into five distinct groups,aligning with the AtJAZ family.Selection pressure analysis revealed positive selection(Ka/Ks>1)acting on CsJAZ1,CsJAZ8,and CsJAZ9,suggesting adaptive roles during tea domestication.Structural variants(SVs)significantly impacted gene expression and structural integrity;notably,CsJAZ4,CsJAZ9,and CsJAZ12 exhibited differential expression when affected by SVs.RNA-seq analysis across four tissues from the 22 tea plant cultivars showed consistently high expression of CsJAZ1,CsJAZ2,CsJAZ6,CsJAZ9,CsJAZ13,and CsJAZ14,highlighting their potential fundamental roles.This study elucidates the JAZ gene family's evolutionary complexity and functional versatility within the tea plant pan-genome.These findings provide valuable insights for future research into CsJAZ functions and serve as a model for pan-genomic analyses of gene families in other plant species. 展开更多
关键词 functional characterization selection pressure jasmonate zim domain genes Camellia sinensis camellia sinensis genomes evolutionary dynamics presence absence variability phylogenetic analysis
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QTL detection and candidate gene analysis of the anthracnose resistance locus in tea plant(Camellia sinensis) 认领 引用 被引量：5
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作者 Chenyu Zhang Hongli Li +6 位作者 Piao Mei Yuanyuan Ye Dingding Liu Yang Gong Haoran Liu Mingzhe Yao Chunlei Ma 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2025年第6期2240-2250,共11页
Anthracnose is a devastating disease caused by Colletotrichum that significantly affects the yield and economic value of the tea plant(Camellia sinensis). However, few studies have addressed the genetic mechanism of a... Anthracnose is a devastating disease caused by Colletotrichum that significantly affects the yield and economic value of the tea plant(Camellia sinensis). However, few studies have addressed the genetic mechanism of anthracnoseresistance(AR). This study investigated the QTL associated with AR in a 'Longjing 43'×'Baijiguan'(LJ43×BJG)population. The field surveys conducted in this study led to the identification of several QTLs for AR on the linkagemap. One major QTL(qAR-12.4) accounted for 12% of the phenotypic variance explained over two years. The BSA-seq results also revealed two genomic regions, q ARChr1 on chromosome 1 and qARChr13 on chromosome 13,which showed strong correlations with AR. Time-course RNA-seq was performed on LJ43 and BJG inoculated withanthracnose at 0, 24, and 48 hours to screen for candidate genes. The results showed the gradual post-inoculationexpression of a nuclear-localized ERF transcription factor(CsERF105) within the qARChr1 locus in BJG but not inLJ43. The AR of BJG was significantly reduced after feeding with CsERF105-specific antisense oligonucleotides,suggesting that CsERF105 may be a positive regulator. The findings of this study add to our general knowledge ofthe genetic factors involved in the tea plant's AR and potential breeding targets. 展开更多
关键词 Camellia sinensis anthracnose genetic map CsERF105 disease resistance
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Characterization of nitrate use efficiency in tea plant(Camellia sinensis)based on leaf chlorate sensitivity 认领 引用 被引量：5
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作者 Wenjing Zhang Xiaoying Dong +3 位作者 Kang Ni Lifeng Ma Lizhi Long Jianyun Ruan 《Horticulture Research》 SCIE CSCD 2025年第4期29-43,共15页
Nitrate(NO3−),a key form of inorganic nitrogen(N)in soils,is typically lost in tea gardens through leaching.However,NO3−utilization efficiency(NiUE)and its characteristic mechanism in tea plants remain unc... Nitrate(NO3−),a key form of inorganic nitrogen(N)in soils,is typically lost in tea gardens through leaching.However,NO3−utilization efficiency(NiUE)and its characteristic mechanism in tea plants remain unclear.This study screened contrastive genotypes of NiUE using leaf chlorate sensitivity and explored the potential genes that regulate this process.Fresh branches of 10 cultivars were hydroponically cultivated and subjected to potassium nitrate(KNO3)and potassium chlorate(KClO3)treatments,with the former as the control group.The sensitive cultivar,Zhenong 117(ZN117),showed a decrease in SPAD and Fv/Fmvalues following KClO3treatment,while the tolerant cultivar,Teiguanyin(TGY),exhibited minimal significant changes.After 5 days of cultivation,the 15N concentration and proportion in new shoots of ZN117 were significantly higher than those in TGY.Transcriptome analysis revealed that the expression of genes responsible for NO3−transport,including the nitrate transporters NRT2.4,NPF4.6,NPF6.1,NPF1.10,and NPF1.11,significantly increased in ZN117 after NO3−supply.Genes involved in NO3−reduction,chlorophyll synthesis,and photosynthesis were progressively induced.Coexpression network analysis indicated that the squamosa promoter-binding protein activated the onset of NO3−signaling,while basic helix–loop–helix transcripts were triggered to higher levels during NO3−supply.This study proposes a rapid characterization method of NiUE in woody plants and a speculative molecular regulatory mechanism for the NO3−transfer and remobilization of tea plants.A set of specific genes involved in NO3−transport,reduction,and mobilization were identified and proposed as marker genes for NiUE in tea plants. 展开更多
关键词 nitrate transporters hydroponically cultivated tea plants Camellia sinensis potassium nitrate kno nitrate use efficiency leaf chlorate sensitivity
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CsNAC17 enhances resistance to Colletotrichum gloeosporioides by interacting with CsbHLH62 in Camellia sinensis 认领 引用 被引量：3
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作者 Rui Han Huiling Mei +8 位作者 Qiwei Huang Cunqiang Ma Yuxin Zhao Anburaj Jeyaraj Jing Zhuang Yuhua Wang Xuan Chen Shujing Liu Xinghui Li 《Horticulture Research》 SCIE CSCD 2025年第2期35-49,共15页
The pathogen Colletotrichum gloeosporioides causes anthracnose,a serious threat to tea trees around the world,particularly in warm and humid regions.RNA-Seq data have previously indicated NAC transcription factors are... The pathogen Colletotrichum gloeosporioides causes anthracnose,a serious threat to tea trees around the world,particularly in warm and humid regions.RNA-Seq data have previously indicated NAC transcription factors are involved in anthracnose resistance,but underlying mechanisms remain unclear.The BiFC,Split-LUC,and Co-IP assays validated the interaction between CsbHLH62 and CsNAC17 identified through yeast two-hybrid(Y2H)screening.CsNAC17 or CsbHLH62 overexpression enhanced anthracnose resistance,as well as enhanced levels of H2O2,hypersensitivity,and cell death in Nicotiana benthamiana.The NBS-LRR gene CsRPM1 is regulated by CsNAC17 by binding directly to its promoter(i.e.CACG,CATGTG),while CsbHLH62 facilitates CsNAC17's binding and increases transcriptional activity of CsRPM1.Additionally,transient silencing of CsNAC17 and CsbHLH62 in tea plant leaves using the virus-induced gene silencing(VIGS)system resulted in decreased resistance to anthracnose.Conversely,transient overexpression of CsNAC17 and CsbHLH62 in tea leaves significantly enhanced the resistance against anthracnose.Based on these results,it appears that CsbHLH62 facilitates the activity of CsNAC17 on CsRPM1,contributing to increased anthracnose resistance. 展开更多
关键词 anthracnose resistance nac transcription factors Camellia sinensis colletotrichum gloeosporioides BHLH transcription factors tea trees RNA seq
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Phosphorylation modification reverses the transcriptional inhibitory activity of CsMYB4a in tea plants(Camellia sinensis) 认领 引用 被引量：2
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作者 Guoliang Ma Yunyun Han +2 位作者 Tongtong Li Tao Xia Liping Gao 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第1期406-416,共11页
The members of the fourth subgroup of R2R3-MYB(Sg4 members)are well-known inhibitors of phenylpropanoid and lignin synthesis pathways.The C2 domain is closely related to the transcriptional inhibitory activity of Sg4 ... The members of the fourth subgroup of R2R3-MYB(Sg4 members)are well-known inhibitors of phenylpropanoid and lignin synthesis pathways.The C2 domain is closely related to the transcriptional inhibitory activity of Sg4 members.Phosphorylation modification enhances the transcriptional inhibitory activity of Sg4 members.Here,we identified a phosphorylation site on the C2 domain of Cs MYB4a from tea plants(Camellia sinensis).A mitogen-activated protein kinase(MAPK),named Cs MPK3-2,phosphorylated this site on the C2 domain of Cs MYB4a.Further experiments revealed that phosphorylation of Cs MYB4a weakened its ability to inhibit the gene expression of PAL,C4H,and 4CL in the phenylpropanoid pathway and activated the expression of transcription factor YABBY5,maintaining the adaxial-abaxial polarity of the leaf.Knocking out Nt YAB5 in Cs MYB4a transgenic tobacco partially repaired the leaf wrinkling phenotype caused by Cs MYB4a.The C1 domain exhibited an activation function when the C2 domain of Cs MYB4a was phosphorylated by Cs MPK3-2,causing this reversal phenomenon.These results enrich our understanding of the regulatory diversity of Sg4 members. 展开更多
关键词 Camellia sinensis Cs MYB4a Phenylpropanoid pathway MAPK YABBY Leaf development
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Composition,biosynthesis and pharmacological activities of chemical constituents of Ophiocordyceps sinensis:a review 认领 引用
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作者 Wenting Wang Bohan Cheng +8 位作者 Haotian Wu Yuhua Shi Lan Wu Qinggang Yin Mengyue Wang Yuanzhu Liu Ling Yuan Ranran Gao Li Xiang 《Medicinal Plant Biology》 2025年第1期163-176,共14页
Ophiocordyceps sinensis(syn.DongChongXiaCao),a rare and endemic medicinal fungus native to China,possesses significant pharmacological and commercial value.However,the chemical composition,pharmacological mechanisms,a... Ophiocordyceps sinensis(syn.DongChongXiaCao),a rare and endemic medicinal fungus native to China,possesses significant pharmacological and commercial value.However,the chemical composition,pharmacological mechanisms,and biosynthetic pathways of secondary metabolites in O.sinensis remain largely uncharacterized,hindering efforts to enhance its quality and develop derived products.This review summarizes the resource status,chemical constituents,and pharmacological properties of O.sinensis,with a focus on the omics research progress and the biosynthetic pathways of key secondary metabolites such as cordycepin,ergosterol,and polysaccharides.Modern pharmacological studies reveal that O.sinensis exhibits immunomodulatory,antiinflammatory,antitumor,anti-aging,antihypertensive,and antioxidant activities,primarily attributed to its polysaccharides,nucleosides,sterols,and peptides.Despite advancements in omics technologies for studying its unique developmental mechanisms and secondary metabolite biosynthesis,knowledge in this field remains limited,especially compared with congeneric species.Finally,future research directions,including the development of quality biomarkers,exploration of complex macromolecules,investigation of O.sinensis-environment interactions,and artificial cultivation techniques,are proposed to alleviate resource scarcity.This review highlights current research gaps and provides actionable solutions,laying a foundation for the subsequent development of related products and the sustainable utilization of O.sinensis. 展开更多
关键词 secondary metabolites Dongchongxiacao biosynthetic pathways Chemical Constituents chemical compositionpharmacological Ophiocordyceps sinensis ophiocordyceps sinensis syndongchongxiacao pharmacological properties
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Four signal chemicals can non-destructively induce enhanced resistance to Asian citrus psyllids in Citrus sinensis while maintaining balanced plant growth and development 认领 引用
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作者 Wei Wang Chuxiao Lin +3 位作者 Yirong Zhang Shiyan Liu Jiali Liu Xinnian Zeng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2025年第7期2732-2748,共17页
Asian citrus psyllid(ACP)is a significant pest of citrus crops that can transmit citrus Huanglongbing(HLB)by feeding on the phloem sap of citrus plants,which poses a significant threat to citrus production.Volatile si... Asian citrus psyllid(ACP)is a significant pest of citrus crops that can transmit citrus Huanglongbing(HLB)by feeding on the phloem sap of citrus plants,which poses a significant threat to citrus production.Volatile signal chemicals with plant communication functions can effectively enhance the resistance of recipient plants to herbivorous insects with minimal impacts on plant growth.While(E)-4,8-dimethyl-1,3,7-nonatriene(DMNT),(E,E)-4,8,12-trimethyl-1,3,7,11-tridecene(TMTT),(E)-β-caryophyllene,and dimethyl disulfide(DMDS),are known as signaling molecules in guava-sweet orange communication,whether these four chemical signals can enhance the resistance of Citrus sinensis to feeding by ACP adults with no apparent costs in terms of plant growth remains unclear.Therefore,this study measured the effect of non-damaging induction by DMNT,TMTT,(E)-β-caryophyllene,and DMDS on the ability of C.sinensis to resist feeding by ACP,as well as their impacts on the defensive phytochemicals,defensive enzymes,functional nutrients,Photosystem II's utilization and allocation of light energy,photosynthetic pigments,growth conditions,and leaf stomatal aperture in C.sinensis.The results indicate that non-damaging induction by these four chemicals can enhance the activity of the defensive enzyme polyphenol oxidase(PPO)and increase the contents of total phenols,tannins,and terpenoid defensive phytochemicals within C.sinensis,thereby enhancing the resistance of C.sinensis to ACP feeding.Specifically,DMNT and DMDS exhibit more significant effects in inducing resistance compared to TMTT and(E)-β-caryophyllene.The characteristics of chlorophyll fluorescence parameters and changes in photosynthetic pigments in C.sinensis during different post-exposure induction periods revealed these chemicals can maintain the stability of the photosynthetic system in C.sinensis and regulate its capacity to capture,transmit,and distribute light energy,which significantly enhances the non-photochemical quenching ability of C.sinensis.In addition,detailed measurements of the water content,leaf mass per unit area(LMA),functional nutrients(soluble protein,soluble sugar,and amino acids),and stomatal parameters in C.sinensis leaves further indicated that the non-destructive induction by these chemicals can optimize the levels of functional nutrients in C.sinensis,primarily manifesting as the upregulation of soluble sugars,proline,or soluble proteins,and reduction of stomatal area and aperture,which maintains a stable leaf water content and LMA,thereby enhancing resistance to ACP while sustaining the healthy growth of C.sinensis.These results fully substantiate that the non-damaging induction by the signal chemicals DMNT,TMTT,(E)-β-caryophyllene,and DMDS can enhance the resistance of C.sinensis to ACP feeding while maintaining the balance between pest resistance and growth.This balance prevents any catastrophic effects on the growth of C.sinensis,so these agents can potentially be integrated with other pest management strategies for the collective protection of crops.This study provides theoretical support and assistance for the development of signal chemical inducers for the prevention and management of ACP in agricultural systems. 展开更多
关键词 volatile signals defense priming Asian citrus psyllid resistance Citrus sinensis defensive metabolites physiology and biochemistry
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In-depth exploration of bioactive constituents,biosynthetic pathways,and pharmacological mechanisms of Angelica sinensis:implications for therapeutic development 认领 引用
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作者 Xiumin Zhang Lijun Fu +3 位作者 Ruimei Zhang Li Liu Yongzheng Ma Na Zhang 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第2期404-420,共17页
Angelica sinensis,a well-known traditional Chinese medicinal herb with the unique property of being both a medicine and an edible plant,has been widely used for promoting blood circulation,modulating immunity,and reli... Angelica sinensis,a well-known traditional Chinese medicinal herb with the unique property of being both a medicine and an edible plant,has been widely used for promoting blood circulation,modulating immunity,and relieving pain.This review comprehensively investigates the extraction methods,structural characteristics,and biological activities of its primary bioactive components,such as polysaccharides,volatile oils,organic acids,and flavonoids.The biosynthesis pathways of these compounds,along with the key enzymes and transcription factors involved,are investigated to understand the factors influencing their synthesis and accumulation.Additionally,the biological activities of A.sinensis,including hepatoprotective,anti-inflammatory,immunemodulatory,anti-tumor,circulatory benefits,and neuroprotection,along with their underlying mechanisms are introduced.These findings provide a solid foundation for the development of A.sinensis as a valuable resource in functional foods and pharmaceutical products. 展开更多
关键词 Angelica sinensis Bioactive compounds Biosynthesis pathways Biological activity Functional foods
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Gene expression profiles of Chinese medaka (Oryzias sinensis) primary hepatocytes in response to estrone (E1), 17β-estradiol (E2) and estriol (E3) 认领 引用
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作者 Yue Wang Junhui Lv +7 位作者 Zhongtang Xie Narma Huai Kailun Zhang Ying Zhou Yilihamu Reze Hua Zhu Xiqing Li Zhaobin Zhang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2025年第6期368-375,共8页
The natural estrogens,including estrone(E1),17β-estradiol(E2),and estriol(E3),are frequently detected in aquatic environment at relatively high levels.The most commonly used biomarkers for estrogens are main... The natural estrogens,including estrone(E1),17β-estradiol(E2),and estriol(E3),are frequently detected in aquatic environment at relatively high levels.The most commonly used biomarkers for estrogens are mainly expressed in the liver of fish.Analyses of the global gene profiling in fish liver cells under estrogens treatment will provide precise toxicogenomic information of the natural estrogens which is still not well known.In this study,we developed methods for isolation and culture of primary hepatocytes from liver tissue of male Chinese medaka(Oryzias sinensis),and analyzed the global gene expression profiling in the primary hepatocytes treated with E1(1,10,and 100 nmol/L),E2(0.01,0.1,1,10,and 100 nmol/L),and E3(1,10,and 100 nmol/L)using RNA-seq.It was found that 175,248,and 218 genes were differentially expressed in the E1,E2,and E3 groups,respectively.These differentially expressed genes(DEGs)were mainly enriched in Gene Ontology(GO)terms of“response to estradiol”,“response to estrogen”,and“lipid transport”.Of the DEGs,vitellogenin genes,including vtg1,vtg2,and vtg3,were the mostly up-regulated and followed by zona pellucida genes which include zp2.3,zp2l1 and zp3a.2.In addition,genes of slc41a1,zp2.1,esr1,pkd1l1,fam20ca,best3,etc.were also obviously up-regulated by the estrogens in concentration-dependent patterns.RT-qPCR was used to validate the results of RNA-seq and found that vtg2 should be the best biomarker gene for estrogen study,which could well response to natural estrogens and weak estrogenic chemical,propyl 4-hydroxybenzoate. 展开更多
关键词 Oryzias sinensis Primary hepatocytes Natural estrogens Vitellogenin,toxicogenomics
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Studies on the anti-hair loss mechanism of Aquilaria sinensis leaf extract by integrated metabolomics and network pharmacology 认领 引用
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作者 Zhengang Peng Zhengwan Huang +1 位作者 Zhe Liu Xiaoxiao Lin 《日用化学工业（中英文）》 CAS 北大核心 2025年第6期767-778,共12页
The anti-hair loss mechanism of Aquilaria sinensis leaf extract(ASE)has been studied by using metabolomics and network pharmacology.Metabolomics was utilized to comprehensively identify the active constituents of ASE,... The anti-hair loss mechanism of Aquilaria sinensis leaf extract(ASE)has been studied by using metabolomics and network pharmacology.Metabolomics was utilized to comprehensively identify the active constituents of ASE,and the network pharmacology was used to elucidate their anti-hair loss mechanism,which was verified by molecular docking technology.572 active compounds were identified from the ASE by metabolomics methods,where there are 1447 corresponding targets and 492 targets related to hair loss,totaling 88 targets.20 core active substances were identified by constructing a network between common targets and active substances,which include vanillic acid,chorionic acid,caffeic acid and apigenin.The five key targets of TNF,TP53,IL6,PPARG,and EGFR were screened out by the PPI network analysis on 88 common targets.The GO and KEGG pathway enrichment analysis showed that the inflammation,hormone balance,cell growth,proliferation,apoptosis,and oxidative stress are involved.Molecular docking studies have confirmed the high binding affinity between core active compounds and key targets.The drug similarity assessment on these core compounds suggested that they have the potential to be used as potential hair loss treatment drugs.This study elucidates the complex molecular mechanism of ASE in treating hair loss,and provides a reference for the future applications in hair care products. 展开更多
关键词 metabolomics network pharmacology hair loss Aquilaria sinensis leaf extract molecular docking
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Mechanisms of silicon-mediated amelioration for Camellia sinensis using physiology,transcriptomics,and metabolomics under cold stress 认领 引用
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作者 Xueying Xie Shuqing Yang +2 位作者 Xiaoxia Zhao Tao Shang Xiaoyang Han 《Beverage Plant Research》 CAS 2025年第1期359-369,共11页
Cold stress significantly impacts the growth,quality,and yield of tea plant leaves.Additionally,silicon(Si)is a widely abundant element on earth and is recognized for its beneficial effect on plant growth and developm... Cold stress significantly impacts the growth,quality,and yield of tea plant leaves.Additionally,silicon(Si)is a widely abundant element on earth and is recognized for its beneficial effect on plant growth and development.Here,a comprehensive transcriptomic,biochemical,and metabolomic investigations on tea plant leaves was conducted,examining the impact of Si-mediated improvement during short-(1 d),medium-(3 d),and long-term(5 d)exposure to cold stress.Biochemical and physiological analyses demonstrated that Si-mediated amelioration enhanced the activity of protective enzyme systems during all three cold stress treatments.There was a marked increase in soluble sugar content and a significant decrease in chlorophyll B content during the medium-term cold stress.Integrated transcriptomic and metabolomic analyses revealed that the differentially expressed genes(DEGs)primarily targeted pathways in phenylpropane biosynthesis,α-linolenic acid metabolism,pentose and glucuronic acid interconversion,and nitrogen metabolism.Similarly,differentially abundant metabolites(DAMs)linked to cold stress response in tea plants predominantly involved flavone and flavonol biosynthesis,flavonoid biosynthesis,aminoacyl-tRNA biosynthesis,galactose metabolism,and phenylpropanoid biosynthesis.The integrated analysis of the metabolites associated with these pathways and expression profiles further confirmed the involvement of the identified DEGs in responding to cold stress and the beneficial effect of Si in tea plants.This research expands our knowledge of the regulatory networks underlying cold stress responses in tea plants. 展开更多
关键词 silicon metabolomics cold stressbiochemical physiological analyses Camellia sinensis physiology cold stress tea plant leaves transcriptomics
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Multiple distinctive lineages of Camellia sinensis var.assamica from Indiaand China revealed by Single Nucleotide Polymorphism markers 认领 引用
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作者 Devajit Borthakur Boyi Wang +6 位作者 Lyndel WMeinhardt Lin Zhou Huawei Tan Anup Kumar Barooah Sangeeta Borchetia Wanping Fang Dapeng Zhang 《Beverage Plant Research》 CAS 2025年第1期146-155,共10页
Knowledge of genetic diversity is fundamental for improving conservation and utilization of tea plant genetic resources.One hundred and fifty SNP markers were used to assess the genetic diversity in 252 tea accessions... Knowledge of genetic diversity is fundamental for improving conservation and utilization of tea plant genetic resources.One hundred and fifty SNP markers were used to assess the genetic diversity in 252 tea accessions covering a wide range of geographical origins including Assam India,Southwest and Southeast Yunnan and Eastern China.Multivariate and Bayesian clustering analysis revealed six distinctive genetic lineages,corresponding to Camellia sinensis var.sinensis type and five C.sinensis var.assamica from India,Indochina,Southwest Yunnan(China),and Southeast Yunnan(China).Fst among these different lineages ranges from 0.161 to 0.493 and all are highly significant(p<0.001).The present study suggests that there are at least five different genetic populations of var.assamica in their primary gene pool,including:(1)India assamica,(2)Cambod(or Southern form lasiocalyx),(3)Southwest Yunnan assamica,(4)Jinping,Yunnan assamica,and(5)Malipo,Yunnan assamica.We further suggest that the populations of var.assamica were independently domesticated based on the indigenous wild tea plants,rather than introduced cultigens from a single origin.Geographically,the five var.assamica populations could be grouped into two large clusters.The West cluster includes India assamica,lasiocalyx and Southwest Yunnan assamica,whereas the East cluster includes Southeast Yunnan assamica.Our results also support the old postulate that Cambod type var.assamica is a distinctive member of C.sinensis var.assamica.Inferred parentage in hybrid breeding lines from India showed that there was no introgression of Chinese assamica germplasms,suggesting the potential of exploring inter-population heterosis using the untapped germplasm. 展开更多
关键词 genetic diversity tea accessions Camellia sinensis Conservation snp markers bayesian clustering analysis conservation utilization
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20-Hydroxyecdysone Affects Hepatopancreatic Vitellogenin Synthesis in Chinese Mitten Crabs(Eriocheir sinensis)Through the PLC-PKC Pathway 认领 引用
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作者 CHENG Runqiang GEN Changsheng LI Ye 《Journal of Ocean University of China》 SCIE CAS CSCD 2025年第6期1807-1817,共11页
In crustaceans,20-hydroxyecdysone(20E),the primary bioactive form of ecdysteroid hormones,regulates molting and ovarian development.In vivo and in vitro approaches were used to examine the potential nongenomic mechani... In crustaceans,20-hydroxyecdysone(20E),the primary bioactive form of ecdysteroid hormones,regulates molting and ovarian development.In vivo and in vitro approaches were used to examine the potential nongenomic mechanisms through which 20E modulates ovarian maturation in Chinese mitten crabs(Eriocheir sinensis)and its effects on signaling components and associated genes within the phospholipase C(PLC)-protein kinase C(PKC)pathway.The results demonstrate that the administration of 20E considerably upregulated the hepatopancreatic mRNA expression of G proteins(Gs and Gq),calmodulin-dependent protein kinase II,and inositol 1,4,5-trisphosphate receptors.Concurrent increases in phosphatidylinositol-specific PLC activity were observed,along with elevated levels of its catalytic products(i.e.,inositol 1,4,5-trisphosphate and diacylglycerol).Moreover,20E stimulation activated the phosphorylation and enhanced the transcriptional expression of PKC.Notably,in vitro pharmacological inhibition with U73122(a PLC inhibitor)and Go6983(a PKC inhibitor)resulted in the effective suppression of 20E-induced expression of downstream target genes,including the ecdysone receptor(Ec R),retinoid X receptor(RXR),vitellogenin(Vg),and Vg receptor(VgR).These findings suggest that 20E modulates hepatopancreatic Vg synthesis in the Chinese mitten crab through PLC-PKC signaling transduction,thereby exerting indirect regulatory effects on ovarian development. 展开更多
关键词 Eriocheir sinensis 20-hydroxyecdysone vitellogenin GPCR-PLC-PKC pathway
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Mechanism prediction of Astragalus mongholicus Bunge and Angelica sinensis Diels in treating interstitial lung disease based on network pharmacology and molecular docking 认领 引用
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作者 Jun Du Jian Hao Wei Wei 《TMR药理学研究》 2023年4期1-9，共9页
Objective:To investigate the mechanism by which Astragalus mongholicus Bunge(AM),and Angelica sinensis Diels(AS)act in interstitial lung disease(ILD)based on computational prediction.Methods:We screened the ingredient... Objective:To investigate the mechanism by which Astragalus mongholicus Bunge(AM),and Angelica sinensis Diels(AS)act in interstitial lung disease(ILD)based on computational prediction.Methods:We screened the ingredients of AM and AS in PubMed,the Web of Science,China National Knowledge Infrastructure(CNKI)Databases,etc.Then obtained the potential effective components.By sharing the same molecular with ILD,we got the possible target genes for ILD treatment and constructed components–targets–disease network with Cytoscape software.The CTD(Comparative Toxicogenomics Database)database was used for GO and KEGG enrichment analysis of these target genes.Results:59 active ingredients that can be druggable were chosen from AM,67 active ingredients were chosen from AS.77 overlapping target genes for AM and ILD and 36 overlapping target genes for AS and ILD were acquired.The hub targets of AM were PTGS2,PTGS1,CDK2,MAOA,ESR1,TOP2A,GSK3B,ESR2,PPARG,NOS2,The hub targets of AS were PTGS2,GABRA1,PTGS1,CHRM1,SLC6A2,ADRA1B,ADRAIA,ADRB2,CHRM3,GABRA2,CHRM2.Quercetin,kaempferol,daidzein,pavilion,7-Hydroxycoumarin,and 5-Hydroxycoumarin were the main active ingredients which have more effective targets.Prediction of the protein-protein interaction network showed PTGS2,GSK3B,PPARG,etc.,were the important predicted targets.The enriched KEGG pathways,including the Immune System,Metabolism of lipids and lipoproteins,Cytokine Signaling in the Immune system,Generic Transcription Pathway,The interleukin pathway,Metabolism of proteins,PI3K-Akt signaling pathway,Metabolic pathways,Innate Immune System,Neuroactive ligand-receptor interaction,Metabolism,GPCR downstream signaling,Amine ligand-binding receptors,Class A/1,Calcium signaling pathway.Molecular docking showed that quercetin,kaempferol,daidzein,pavilion,7-Hydroxycoumarin,5-Hydroxycoumarin had good binding activities with PTGS2 and GSK3B,which mainly mediated PI3K/Akt and other important signaling pathways in the pathogenesis of ILD.Conclusion:The components in AS and AM share some common targets,such as PTGS2.AM and AS may ameliorate ILD through the PI3K-Akt signaling pathway which is mediated by GSK3B.PTGS2,PPARG may also be vital target genes in the treatment of ILD with AM and AS. 展开更多
关键词 Astragalus mongholicus Bunge Angelica sinensis Diels computational prediction interstitial lung disease PI3K-Akt signaling pathways
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