Label-free super-resolution imaging based on the spatial-frequency-shift(SFS)effect enables conventional microscopes to surpass the diffraction limit,holding significant promise for nanoscale inspection in materials s...Label-free super-resolution imaging based on the spatial-frequency-shift(SFS)effect enables conventional microscopes to surpass the diffraction limit,holding significant promise for nanoscale inspection in materials science and biology.However,current SFS approaches generally face a practical trade-off in obtaining both the ultra-high resolution and the high signal-to-noise ratio(SNR)when using high lateral wavevector(kx)illumination supported by a natural waveguide or single metal film.Here,we proposed a wavevector resonance modulation scheme in multilayered nanostructures that introduces an enhanced deep SFS effect,which is realized by surface plasmon polariton illumination supported by a designed multilayer with relaxed fabrication tolerance.This approach simultaneously achieves a peak-topeak distance of∼70 nm under the excitation wavelength of 780 nm,a resonance-enhanced illumination field,and a more than 10-fold expansion for the coherent transfer function(CTF).We demonstrated the simple excitation process by means of gratings and presented an application in nano-imaging experiments for label-free particles.Results show the advance in the resonance-enhanced illumination modulation method for super-resolution imaging,enabling conventional microscopes to detect and distinguish label-free nanoscale structures with characteristic lateral scales down to sub-λ∕10.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.T2525010,T2293751,and 2024YFF1206700)the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(Grant No.2024R01001).
摘要Label-free super-resolution imaging based on the spatial-frequency-shift(SFS)effect enables conventional microscopes to surpass the diffraction limit,holding significant promise for nanoscale inspection in materials science and biology.However,current SFS approaches generally face a practical trade-off in obtaining both the ultra-high resolution and the high signal-to-noise ratio(SNR)when using high lateral wavevector(kx)illumination supported by a natural waveguide or single metal film.Here,we proposed a wavevector resonance modulation scheme in multilayered nanostructures that introduces an enhanced deep SFS effect,which is realized by surface plasmon polariton illumination supported by a designed multilayer with relaxed fabrication tolerance.This approach simultaneously achieves a peak-topeak distance of∼70 nm under the excitation wavelength of 780 nm,a resonance-enhanced illumination field,and a more than 10-fold expansion for the coherent transfer function(CTF).We demonstrated the simple excitation process by means of gratings and presented an application in nano-imaging experiments for label-free particles.Results show the advance in the resonance-enhanced illumination modulation method for super-resolution imaging,enabling conventional microscopes to detect and distinguish label-free nanoscale structures with characteristic lateral scales down to sub-λ∕10.