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GDE-YOLO:a robust and accurate method for real-time tea leaf disease detection in complex plantation environments 认领 引用
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作者 Lei GUO Hongbin CHEN +6 位作者 Zheming CUI Zhenhui LAI Weiliang HUANG Tianliang LIN Jufei WANG Shuhe ZHENG Wuxiong WENG 《ENGINEERING Agriculture》 CAS CSCD 2026年第4期23-44,共22页
To address the inherent problems of high labor costs and poor efficiency of current visual diagnosis methods for tea leaf diseases,this study proposes a GDE-YOLO-based real-time detection method for tea leaf diseases ... To address the inherent problems of high labor costs and poor efficiency of current visual diagnosis methods for tea leaf diseases,this study proposes a GDE-YOLO-based real-time detection method for tea leaf diseases identification in complex tea plantation environments.The proposed architecture integrates three key enhancements:(1)combination of the neck network with a global attention mechanism(GAM),(2)optimization of the C2f module through a diverse branch block(DBB),and(3)replacement of a complete intersection over union loss function with an efficient intersection over union loss function,collectively improving recognition accuracy and speed.Experimental validation demonstrates that GDE-YOLO achieved 91.7%precision(3.1%higher than YOLOv8n)across different tea plantation scenarios and disease types,with specific improvements of 0.7%for tea anthracnose and 12.4%for tea white spot detection.Also,the enhanced model attained 80 FPS real-time performance.The deployment test on the NVIDIA Jetson Orin Nano edge device showed that GDE-YOLO could achieve precise diseases identification with confidence threshold>0.8 and inference speed maintaining 18 FPS,satisfying edge computing requirements of accuracy and real-time performance.This research provides critical technical foundations for vision-guided precision sprayers in tea plantations,while promoting the practical implementation of machine vision in intelligent agricultural management. 展开更多
关键词 Attention mechanism deep learning precision agriculture tea leaf diseases YOLOv8
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Enhancing Tea Leaf Disease Classification with Cross-Attention Fusion and Magnitude-Aware Linear Attention 认领 引用
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作者 Jiaxin Zhu 《Journal of Electronic Research and Application》 2026年第3期14-20,共7页
Accurate tea leaf disease classification in real-world scenarios is hindered by complex backgrounds and the loss of fine-grained lesion details during CNN down sampling.To address this,we propose ResNet50-Dual-Fusion.... Accurate tea leaf disease classification in real-world scenarios is hindered by complex backgrounds and the loss of fine-grained lesion details during CNN down sampling.To address this,we propose ResNet50-Dual-Fusion.It integrates a Cross-Attention Feature Fusion module(CAmodule)to adaptively reconstruct tiny lesion edges via cross-spatial interaction between shallow and deep features.Furthermore,a Magnitude-Aware Linear Attention(MALA)module with 2D Rotary Position Embedding(RoPE)is introduced to rectify magnitude neglect,effectively suppressing background noise.Evaluated on a 5,276-image dataset,our model achieves 85.96%accuracy(+3.00%over the baseline),outperforming architectures like ViT and Swin-Tiny.Grad-CAM visualizations confirm its superior lesion localization,providing a robust paradigm for automated crop disease diagnosis. 展开更多
关键词 Tea leaf disease classification Deep learning Residual network(ResNet-50) Cross-attention mechanism Magnitude-aware linear attention(MALA)
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CsMYB75–CsTT8–CsTTG1 protein complex activates CsANS3 for anthocyanin biosynthesis in purple-leaf tea plants 认领 引用
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作者 Li Zhang Hui Xie +13 位作者 Yue Hu Hui Wang Linlin Xu Yongning Tao Lingling Tao Hongrong Chen Xueming Ma Honglian Gu Deyu Xie Jianbing Hu Shengrui Liu Junyan Zhu Kun Dong Chaoling Wei 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第8期1985-1989,共5页
Purple-leaf tea plants are rich in anthocyanins,which have antioxidant,antimutagenic,and antiaging activities.Extensive research has elucidated the anthocyanin biosynthetic pathways and regulatory role of CsMYB75 in c... Purple-leaf tea plants are rich in anthocyanins,which have antioxidant,antimutagenic,and antiaging activities.Extensive research has elucidated the anthocyanin biosynthetic pathways and regulatory role of CsMYB75 in common green-leaf tea plants;however,the precise molecular mechanism through which CsMYB75 regulates anthocyanin synthesis in purple-leaf tea plants has yet to be fully elucidated.In this study,RNA-seq was performed on the buds and the first to fourth leaves of the purple-leaf tea cultivar‘Zijuan’(Camellia sinensis var.assamica).Weighted gene coexpression network analysis(WGCNA)revealed that the expression of CsMYB75 was not only significantly correlated with anthocyanin accumulation but also strongly correlated with the expression of the anthocyanin synthase gene CsANS3.Further experiments demonstrated that CsMYB75 binds to the MYB-binding site(CAACCA)in the CsANS3 promoter region and positively regulates its expression.Moreover,the protein–protein interactions of CsMYB75,CsTT8,and CsTTG1 resulted in the formation of a multimeric complex(CsMYB75–CsTT8–CsTTG1).This complex exhibits the strongest transcriptional activation of the CsANS3 promoter,with activation levels that are 4.1–4.8-fold higher than those observed with any pairwise combination of the three transcription factors.Overall,the results of this study elucidate the mechanism of CsMYB75 in regulating anthocyanin biosynthesis in purple-leaf tea plants during different developmental stages and provide valuable genetic resources and a theoretical foundation for the molecular breeding and metabolic engineering of tea plants. 展开更多
关键词 csmyb transcriptional activation WGCNA molecular breeding molecular mechanism anthocyanin biosynthesis csans purple leaf tea plants
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Polysaccharides from large leaf yellow tea activates the LXRα-ABCA1/ABCG1 pathway to ameliorate type 2 diabetes by regulating gut microbiota 认领 引用
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作者 Hao Chen Xinxin Pei +7 位作者 Zhuang Wang Jielin Chen Kaili Xuan Lei Gong Xinyu Zhou Yijun Wang Yuzhe Huang Yan Chen 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第4期1674-1687,共14页
Type 2 diabetes mellitus(T2DM)is a multifaceted metabolic condition characterized by chronic hyperglycemia(elevated blood sugar levels)and insulin resistance(IR).We have isolated a new homogeneous polysaccharide,LYTP-... Type 2 diabetes mellitus(T2DM)is a multifaceted metabolic condition characterized by chronic hyperglycemia(elevated blood sugar levels)and insulin resistance(IR).We have isolated a new homogeneous polysaccharide,LYTP-3(7.22×10~5 Da),with anti-diabetic activity,from large leaf yellow tea(LYT)by biological activityguided sequential separation and activity evaluation.Using a T2DM mouse model,we demonstrate that oral administration of LYTP-3 could reduce blood lipid levels,alleviate hyperglycemia and glucose tolerance,and inhibit IR.LYTP-3 ameliorated T2DM treatment by activating the liver X receptor alpha(LXRα)-ATPbinding cassette(ABC)transporter(LXRα-ABCA1/ABCG1)signaling pathway,which is regulated by the gut microbiota.The strain Lachnospiraceae bacterium A4 is potentially the key to this improvement.Additionally,we have solved the molecular structure of LYTP-3 using nuclear magnetic resonance(NMR),and methylation analyses.The main backbone of LYTP-3 consists of 1,2,4-α-L-Rhap,1,4-α-D-Galp,1,6-α-D-Glcp,with branches of 1,4-β-D-Galp,1,6-β-D-Galp A,1,3,6-β-D-Manp,1,2-α-L-Rhap,T-β-Manp,and T-β-Galp.The findings of this study suggest that LYTP-3 may serve as a potential gut microbiota modulator in the treatment of T2DM. 展开更多
关键词 Lachnospiraceae bacterium LXRα-ABCA1/ABCG1 signaling pathway Type 2 diabetes mellitus Large leaf yellow tea polysaccharide
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Comparative study on Leaf Tissue Morphologic Changes of Chang-Sheng-Chuan Green Brick Tea Before ad After Fermentation 认领 引用
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作者 何建刚 肖长义 +3 位作者 王春燕 李世刚 王慧 柳蔚 《Agricultural Science & Technology》 CAS 2017年第3期492-495,共4页
[Objective] The aim was to understand the change of fermentation pro- cess of Chang-sheng-chuan green brick tea and analyze the changes in the con- tents of structure components of tea leaves before and after fermenta... [Objective] The aim was to understand the change of fermentation pro- cess of Chang-sheng-chuan green brick tea and analyze the changes in the con- tents of structure components of tea leaves before and after fermentation. [Method] Ten tea leaves before and after fermentation were chosen. The tea leaves sections were made using paraffin microtomy, PAS staining, and observed under light mi- croscopy. Through image analysis for the tea leaves, we compared the changes on the areas of the cellulose and colloid in cell before and after pile-fermentation. At the same time, the tea leaves were observed under transmission electron micro- scope. [Result] The results showed that the pile-fermentation process caused the tea leaves tissue to become loose, and expanded the cell space. Besides, the cell wall of tea leaves was obviously thinning, and even breaking which composed of cellulose. Image analysis showed that the leaf fiber composition reduced significant- ly, with a corresponding increase of yellow colloid in cell. Under observation of transmission electron microscope, there were large amounts of fungi located in the space between the colloid and the cell wall of the tea leaves after pile-fermentation. [Conclusion] The fermentation of Green brick tea is the process of microbial bio- transformation of tea, which is dominated by microbe and consumed the cellulose components of tea leaves. 展开更多
关键词 Green Brick tea Tea leaf Structure Fermentation
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Characterization of mulberry leaf instant tea and evaluation of its hypolipidemia effect via regulation of intestinal microbiota 认领 引用 被引量:5
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作者 Xiaoyun Han Yunlong Bai +3 位作者 Xiaoxin Feng Baochang Du Baojiang Zheng Qingshen Sun 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第3期1348-1357,共10页
The purpose of this study was to characterize mulberry leaf instant tea(MLIT)powder prepared from the'Longsang No.1'(Morus abla L.cv.Longsang 1)mulberry leaves in Heilongjiang Province(China)and assess its obe... The purpose of this study was to characterize mulberry leaf instant tea(MLIT)powder prepared from the'Longsang No.1'(Morus abla L.cv.Longsang 1)mulberry leaves in Heilongjiang Province(China)and assess its obesity-preventingelieving effects.A total of 174 compounds including quercetin,chlorogenic acid,1-deoxyecomycin(1-DNJ)related to antihyperlipidemia effects were identified from the MLIT powder.MLIT treatment reversed the Lee's index,fat coefficient,and serum biochemical parameters in both the obesity relieving and obesity preventing mice fed with high-fat diet.In the obesity relieving experiment,the relative abundance of Desulfovibrio in mouse feces decreased after both 0.5%and 1%MLIT treatments.In obesity preventing experiments,mouse with different amount of MLIT treatments showed increased relative abundance of Akkermansia,Bifidobacterium and Lactobacillus,while Deferribacteres,Desulfobacterota decreased.The beneficial bacteria in the intestinal tract of mice treated with MLIT increased.This study proved that MLIT had antihyperlipidemia potential via modulating intestinal microbiota in mice. 展开更多
关键词 Mulberry leaf instant tea Liquid chromatography-mass spectrometry(LC-MS) Antihyperlipidemia Intestinal microbiota
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不同处理清香型铁观音茶感官品质及生化成分与香气成分分析 认领 引用 被引量:3
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作者 周炎花 冯花 +4 位作者 杨双旭 沈丹玉 谢杰全 郭雅玲 王飞权 《现代食品科技》 CAS 北大核心 2026年第5期346-356,共11页
为探究不同摇青处理对清香型铁观音单叶茶品质的影响,以中开面三叶嫩梢四次摇青(CK1)和单叶四次摇青(CK2)为对照,分别设置单叶2次摇青(DY1)和单叶3次摇青(DY2)处理,对四种处理所制茶叶的感官品质、主要生化成分和香气成分进行了比较。... 为探究不同摇青处理对清香型铁观音单叶茶品质的影响,以中开面三叶嫩梢四次摇青(CK1)和单叶四次摇青(CK2)为对照,分别设置单叶2次摇青(DY1)和单叶3次摇青(DY2)处理,对四种处理所制茶叶的感官品质、主要生化成分和香气成分进行了比较。结果表明,CK2感官品质综合得分(93.10分)显著高于其他处理(P1、P10为筛选条件,确定芳樟醇、吲哚、橙花叔醇等11种组分为导致样本间香气品质差异的关键呈香物质。本研究结果为清香型铁观音单叶茶的工艺创新与品质提升提供了理论依据。 展开更多
关键词 清香型铁观音 单叶茶 感官品质 生化成分 香气成分
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多菌种协同发酵对桑叶茶风味品质及生物活性成分的影响 认领 引用 被引量:2
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作者 梁秀华 何秀玲 +6 位作者 王咪 葛松涛 闻语嫣 江允熙 乐尚坤 董利宁 林杰 《食品与发酵工业》 EI CAS CSCD 北大核心 2026年第10期222-229,I0054-I0058,共8页
微生物发酵对桑叶茶的品质形成具有显著影响。为探究多菌种协同发酵对桑叶茶生物活性成分与风味品质的影响,该研究以桑叶绿茶为原料,分别采用直投式乳酸菌混合发酵(FL)与乳酸菌-酿酒酵母顺序发酵(FLS)2种协同发酵模式,以未发酵(NF)与自... 微生物发酵对桑叶茶的品质形成具有显著影响。为探究多菌种协同发酵对桑叶茶生物活性成分与风味品质的影响,该研究以桑叶绿茶为原料,分别采用直投式乳酸菌混合发酵(FL)与乳酸菌-酿酒酵母顺序发酵(FLS)2种协同发酵模式,以未发酵(NF)与自然发酵(FNL)为对照。采用计算机视觉分析、生物活性成分测定和顶空固相微萃取-气相色谱-质谱联用等技术,系统解析桑叶在发酵过程中的品质与成分变化,并通过正交偏最小二乘判别分析与相对气味活度值(relative odor activity values,ROAV)筛选关键差异挥发性成分。结果表明,多菌种协同发酵(FL、FLS)有效消除了桑叶青草味,茶汤色泽转为嫩黄明亮,并形成酸菜香、泡菜香和菌香等复合香型;发酵处理显著提升了总生物碱、1-脱氧野尻霉素(1-deoxynojirimycin,DNJ)和γ-氨基丁酸(γ-aminobutyric acid,GABA)等功能成分含量,其中FL的DNJ含量最高,FLS的GABA含量最为突出,但总黄酮与总多酚含量有所降低;挥发性成分分析表明,与NF及FNL相比,FL与FLS的挥发性物质种类与总量均显著增加;基于ROAV分析,从所有样品中共鉴定出3-辛烯-2-酮、(E)-2-壬烯醛等9种关键香气物质,并明确其香型贡献。该研究阐明了多菌种协同发酵对桑叶茶风味特征与功能成分的调控效应,可为高品质桑叶茶的开发提供理论依据。 展开更多
关键词 多菌种协同发酵 桑叶茶 生物活性成分 挥发性物质
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加工工艺对桑叶茶品质形成的研究进展 认领 引用 被引量:2
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作者 梁秀华 马焕艳 +2 位作者 张谈捷 李腊梅 林杰 《食品与机械》 CAS CSCD 北大核心 2026年第1期221-229,共9页
桑叶是药食同源的重要原料,富含多种营养活性成分,加工成的桑叶茶具有抗氧化、降血糖、降血脂、抗炎等生理功能,已成为健康食品领域的研究热点。萎凋、杀青、发酵、干燥及胁迫处理等加工工艺会影响桑叶茶的风味品质和营养活性成分的种... 桑叶是药食同源的重要原料,富含多种营养活性成分,加工成的桑叶茶具有抗氧化、降血糖、降血脂、抗炎等生理功能,已成为健康食品领域的研究热点。萎凋、杀青、发酵、干燥及胁迫处理等加工工艺会影响桑叶茶的风味品质和营养活性成分的种类与含量,进而影响产品的营养品质与保健功效。文章重点介绍了萎凋、杀青、发酵、干燥及胁迫处理等加工工艺对桑叶茶感官品质和营养活性成分的影响,并对桑叶茶加工工艺优化、功能性产品开发进行了展望。 展开更多
关键词 加工工艺 桑叶茶 营养成分 活性成分 感官品质
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酸枣叶茶中15种元素含量分析及重金属健康风险评价 认领 引用 被引量:1
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作者 张超 丁敏 +6 位作者 李瑞菊 李霞 李增梅 赵彤 王玉涛 王峰恩 胡大刚 《食品安全质量检测学报》 CAS 2026年第6期28-36,共9页
目的探究酸枣叶茶中多种元素含量并评估其营养特征和重金属污染风险。方法市售7个主产区的酸枣叶茶样品经微波消解前处理后,采用等离子体原子发射光谱法(inductively coupled plasma-atomic emission spectrometry,ICP-AES)、电感耦合... 目的探究酸枣叶茶中多种元素含量并评估其营养特征和重金属污染风险。方法市售7个主产区的酸枣叶茶样品经微波消解前处理后,采用等离子体原子发射光谱法(inductively coupled plasma-atomic emission spectrometry,ICP-AES)、电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICP-MS)和原子荧光光谱法(atomic fluorescence spectrometry,AFS)测定酸枣叶茶中K、Ca、Fe、Na、Mn、Sr、Zn、Cu、Cr、Ni、Pb、As、Se、Cd、Hg等15种元素的含量,并运用单因子污染指数法、内梅罗综合多因子指数法及目标危害系数法对酸枣叶茶中6种重金属(Cu、Cr、Pb、As、Cd、Hg)进行污染评价和健康风险评估。结果酸枣叶茶中所测15种元素平均含量顺序为K>Ca>Fe>Na>Mn>Sr>Zn>Cu>Cr>Ni>Pb>As>Se>Cd>Hg,其中K、Ca、Fe含量较高,三者总量占所测元素总量的97.9%,同时不同产区样品中元素含量普遍具有区域差异性(P<0.05)。6种重金属含量均未超过GB 2762—2022《食品安全国家标准食品中污染物限量》规定限值,单因子污染指数均小于0.6,内梅罗综合污染指数均小于0.7,目标危害系数和总危险系数均小于1。结论在本研究评估模型下,市售酸枣叶茶中重金属污染水平较低,对人体的健康风险在可接受范围内。 展开更多
关键词 酸枣叶茶 重金属 相关性分析 健康风险评价
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ALUMINUM CONTENT OF TEA LEAVES AND FACTORSAFFECTING THE UPTAKE OF ALUMINUM FROMSOIL INTO TEA LEAVES 认领 引用 被引量:8
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作者 XIE Zhong-lei, DONG De-ming, BAO Guo-zhang, WANG Sheng-tian, DU Yao-guo,QIU Li-min (Department of Environmental Science and Engineering, Jilin University, Changchun 130023, P. R. China Department of Chemistry, Northeast Normal University, Changchun 13002 《Chinese Geographical Science》 SCIE 2001年第1期87-91,共5页
Numerous studies indicated that aluminum, the most abundant metallic element within the lithosphere, was considered to be related to some human diseases especially the Alzheimer’s disease. Tea, economically an import... Numerous studies indicated that aluminum, the most abundant metallic element within the lithosphere, was considered to be related to some human diseases especially the Alzheimer’s disease. Tea, economically an important beverage in the world, has been found to contain higher concentration of aluminum than many other drinks and foods. Therefore, tea would be a potentially important source of dietary aluminum. In order to understand the sources of aluminum in tea leaves and factors related with aluminum content of tea leaves, an experiment was designed to investigate the relationships of aluminum in tea leaves with leaf age, soil properties and forms of aluminum in soils. The results showed that there were great distinctions in the concentration of aluminum in tea leaves with different leaf age (Alold leaf> Almature leaf> Alyoung leaf). Moreover, soil pH was the major factor controlling the uptake of aluminum from soil into tea leaves. Furthermore, the content of aluminum in tea leaves was better predicated by the soluble aluminum extracted by 0.02mol/L CaCl2. 展开更多
关键词 aluminum tea leaf soil properties
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气候-土地综合影响的全国中小叶与大叶种茶树种植适宜性区划 认领 引用
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作者 周威 李艳 +7 位作者 陈燕丽 张玉芳 范晓青 胡雪琼 杨凯 任义方 陶瑶 朱秀红 《农业工程学报》 EI CAS CSCD 北大核心 2026年第10期300-310,共11页
为揭示叶型-环境的协同调控机制,基于全国1961—2020年气候、土壤数据及茶园样点资料,利用MaxEnt模型筛选茶树种植的主导环境因子,采用自然间断点法划分不适宜区、次适宜区、适宜区和最适宜区4个等级,厘定不同叶型不同等级的主导因子阈... 为揭示叶型-环境的协同调控机制,基于全国1961—2020年气候、土壤数据及茶园样点资料,利用MaxEnt模型筛选茶树种植的主导环境因子,采用自然间断点法划分不适宜区、次适宜区、适宜区和最适宜区4个等级,厘定不同叶型不同等级的主导因子阈值,结合各省茶园实际种植面积占比、实际边界样点对比以及卫星遥感进行验证。结果表明:1)春霜冻频率、年降水量、6—8月相对湿润度指数、最冷月平均气温、3—9月平均相对湿度、≥10℃活动积温、土壤pH值和海拔是两种叶型茶树的共同主导因子,其中春霜冻频率贡献率最高,约占52%~69%。2)模型预测精度较高,大叶种和中小叶种的AUC(area under curve)分别为0.952、0.893,区划结果与各省茶园面积占比、区划等级及边界样点总体吻合。3)大叶种对热量与湿润度需求更高,种植北界基本稳定在长江中下游;中小叶种具备较强低温耐受性,北界北扩约20~40 km。4)气候变暖推动潜在适宜区北移,但土地因子限制扩展幅度,形成“气候可行-土地受限”的格局。研究结果可为茶树品种选育、种植规划及农业政策制定提供理论支持。 展开更多
关键词 茶树 叶型 种植适宜性 最大熵模型
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沉香叶绿茶加工工艺优化及关键香气物质鉴定 认领 引用 被引量:1
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作者 王丽霞 林授锴 +2 位作者 傅丽君 林素英 许香凝 《莆田学院学报》 2026年第2期52-58,共7页
为了推动沉香茶饮产业创新发展,探索沉香叶绿茶加工工艺技术及工艺参数。以感官评价为指标,筛选沉香叶绿茶加工杀青方法和干燥温度组合;色差计及电子舌评价不同杀青方法和干燥温度组合制作的沉香叶绿茶茶汤色泽和滋味,鉴定最优工艺组合... 为了推动沉香茶饮产业创新发展,探索沉香叶绿茶加工工艺技术及工艺参数。以感官评价为指标,筛选沉香叶绿茶加工杀青方法和干燥温度组合;色差计及电子舌评价不同杀青方法和干燥温度组合制作的沉香叶绿茶茶汤色泽和滋味,鉴定最优工艺组合制作的茶关键香气物质。结果表明:以超声波40℃杀青30 min、50℃初烘60 min、75℃复烘至足干的工艺制作的沉香叶绿茶感官评分最高。不同干燥温度组合制作的茶样味觉差异主要表现在苦味、鲜味、咸味及丰富度上。以最佳工艺组合制作的茶样鉴定出102种香气成分,其中,关键香气物质有(E,E)-2,4-庚二烯醛、2-苯乙醇、壬醛、橙化基丙酮、6-甲基-5-庚烯-2-酮等,主要呈现清香、花果香、木香。本研究优化了沉香叶绿茶加工工艺技术及工艺参数,明确了滋味及香气特征,为沉香叶绿茶工业化生产提供了理论依据。 展开更多
关键词 沉香叶 绿茶 工艺技术 工艺参数 感官品质 色度值 香气成分
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乳酸菌发酵桑叶茶对斑马鱼脂质代谢及肠道菌群的调节作用 认领 引用
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作者 梁秀华 何秀玲 +5 位作者 俞思言 丁力光 张帆 张黎 钟玉 林杰 《饲料研究》 CAS 北大核心 2026年第9期64-71,共8页
试验旨在探究乳酸菌发酵桑叶茶对高脂饮食诱导的斑马鱼脂质代谢及肠道菌群的调节作用。选取受精后5 d的AB系野生型斑马鱼、Tg(mpeg1∶DsRed)巨噬细胞红色荧光标记转基因斑马鱼和Tg(-1.7apoa2∶GFP)肝脏绿色荧光标记转基因品系斑马鱼共1... 试验旨在探究乳酸菌发酵桑叶茶对高脂饮食诱导的斑马鱼脂质代谢及肠道菌群的调节作用。选取受精后5 d的AB系野生型斑马鱼、Tg(mpeg1∶DsRed)巨噬细胞红色荧光标记转基因斑马鱼和Tg(-1.7apoa2∶GFP)肝脏绿色荧光标记转基因品系斑马鱼共1260尾,根据检测指标随机分为9组,所有检测项目均设置生物学重复。正常对照组(Control组)给予养殖水,模型对照组(Model组)交替给予0.2%蛋黄粉(8 h)与养殖水(16 h),阳性对照组(Positive组)给予0.2%蛋黄粉及0.08 mg/L洛伐他汀,桑叶绿茶(NF)低、中、高剂量组分别给予0.2%蛋黄粉及125.0、250.0、500.0 mg/L NF水提液,发酵桑叶茶(FL)低、中、高剂量组分别给予0.2%蛋黄粉及125.0、250.0、500.0 mg/L FL水提液。处理4 d后采样,检测相关指标。结果显示,与Model组相比,FL各剂量组脂质积累水平、巨噬细胞荧光强度、肝脏荧光强度及血清总胆固醇(TC)、甘油三酯(TG)含量显著降低(P<0.05),高密度脂蛋白胆固醇(HDL-C)含量显著升高(P<0.05),FL中、高剂量组的低密度脂蛋白胆固醇(LDL-C)含量显著降低(P<0.05)。FL各剂量组脂蛋白酯酶基因(lpl)表达显著上调(P<0.05),过氧化物酶体增殖物激活受体γ基因(pparg)和脂肪酸合酶基因(fasn)表达显著下调(P<0.05)。FL高剂量组的Shannon指数与Simpson指数显著提高(P<0.05),假单胞菌门、气单胞菌属与未分类肠杆菌科的相对丰度降低,拟杆菌门与弯杆菌属的相对丰度升高。研究表明,FL可有效改善高脂斑马鱼脂质代谢紊乱,调节肠道菌群结构,其作用效果优于NF,以500.0 mg/L的添加量效果较好。 展开更多
关键词 乳酸菌发酵 桑叶茶 斑马鱼 脂质代谢 肠道菌群
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海南大叶茶特征多酚对碳水化合物水解酶的抑制机制 认领 引用
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作者 刘孝泽 张淦淇 +2 位作者 孙若梓莹 刘颖鑫 王露 《食品科学》 EI CAS CSCD 北大核心 2026年第10期39-54,共16页
本研究以海南大叶茶为对象,对其红茶与绿茶进行提取,探究相应水提取物及其特征多酚对碳水化合物水解酶(α-淀粉酶和α-葡萄糖苷酶)的抑制潜力及抑制机制。结果表明,绿茶的总多酚(61.9 mg/g)与总黄酮(28.79 mg/g)含量及其对α-葡萄糖苷酶... 本研究以海南大叶茶为对象,对其红茶与绿茶进行提取,探究相应水提取物及其特征多酚对碳水化合物水解酶(α-淀粉酶和α-葡萄糖苷酶)的抑制潜力及抑制机制。结果表明,绿茶的总多酚(61.9 mg/g)与总黄酮(28.79 mg/g)含量及其对α-葡萄糖苷酶(半抑制浓度(half maximal inhibitory concentration,IC50)=59.10μg/mL)和α-淀粉酶(IC50=14.21 mg/mL)的抑制活性均显著优于红茶。研究鉴定出9种特征多酚,并通过分子对接筛选出表没食子儿茶素、表没食子儿茶素没食子酸酯、原花青素B2、山柰酚-3-O-芸香糖苷与鞣花酸5种核心活性单体,其体外抑制活性和促进3T3-L1细胞葡萄糖消耗的能力均得到验证。机制研究结果显示,海南大叶茶中的茶多酚通过竞争性、非竞争性及混合型等多种方式抑制酶活性;荧光猝灭与分子模拟结果证实,多酚能以静态猝灭机制与酶活性中心(如α-淀粉酶的ASP-300/GLU-233及α-葡萄糖苷酶的GLU-296/ASP-269)通过氢键等作用力形成稳定复合物,其中表没食子儿茶素没食子酸酯-α-淀粉酶与原花青素B2-α-葡萄糖苷酶复合物稳定性最佳。本研究阐明了海南大叶茶(尤其绿茶)中的特征多酚可通过“抑制酶活性-促进消耗”双重途径调控餐后血糖,其核心在于与碳水化合物水解酶活性中心的多机制稳定结合。 展开更多
关键词 海南大叶茶 多酚 α-淀粉酶 α-葡萄糖苷酶 分子对接
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乳酸菌-酿酒酵母顺序发酵桑叶茶的活性成分及降血糖作用 认领 引用
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作者 梁秀华 葛松涛 +4 位作者 何秀玲 王伟毅 董利宁 闻语嫣 范明亮 《蚕业科学》 CAS CSCD 北大核心 2026年第1期57-69,共13页
为探究乳酸菌-酿酒酵母顺序发酵桑叶茶的主要活性成分及其降血糖作用,本研究测定了桑叶绿茶及发酵桑叶茶中多酚、黄酮、多糖、生物碱、1-脱氧野尻霉素和γ-氨基丁酸的含量,并通过高血糖斑马鱼模型评价其对血糖水平、胰岛素含量、糖原合... 为探究乳酸菌-酿酒酵母顺序发酵桑叶茶的主要活性成分及其降血糖作用,本研究测定了桑叶绿茶及发酵桑叶茶中多酚、黄酮、多糖、生物碱、1-脱氧野尻霉素和γ-氨基丁酸的含量,并通过高血糖斑马鱼模型评价其对血糖水平、胰岛素含量、糖原合成以及糖代谢关键基因表达的影响;进一步利用转录组测序筛选差异表达基因,进行GO功能和KEGG通路富集分析。结果显示,与桑叶绿茶相比,发酵桑叶茶中多酚和黄酮含量显著降低(P0.05),而总生物碱、1-脱氧野尻霉素和γ-氨基丁酸含量均显著升高(P<0.01);两种茶均能有效降低高血糖斑马鱼的血糖水平,提高胰岛素和糖原含量,并抑制糖异生关键基因FOXO1、PCK1和G6PC的表达;转录组分析表明,发酵桑叶茶可能通过调控脂质代谢、免疫调节及炎症反应等相关通路,在多靶点、多层次上协同改善糖代谢紊乱。综上,乳酸菌-酿酒酵母顺序发酵可显著改变桑叶茶的活性成分组成,其降血糖效果优于桑叶绿茶,具备开发为天然降血糖产品的潜力。 展开更多
关键词 桑叶茶 微生物发酵 1-脱氧野尻霉素 降血糖作用 斑马鱼模型
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云南大叶种白茶研究进展 认领 引用
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作者 刘学艳 王绍梅 +3 位作者 徐婷 龚正礼 罗正飞 刘艳红 《中国茶叶》 2026年第3期38-43,共6页
云南大叶种白茶作为我国茶叶市场的后起之秀,在品饮价值、保健功效与文化赋能方面展现出显著潜力。文章基于文献资料,系统梳理了适制白茶的云南大叶种茶树资源,以及云南白茶加工技术、理化成分与品质特征等方面的研究进展;剖析了当前产... 云南大叶种白茶作为我国茶叶市场的后起之秀,在品饮价值、保健功效与文化赋能方面展现出显著潜力。文章基于文献资料,系统梳理了适制白茶的云南大叶种茶树资源,以及云南白茶加工技术、理化成分与品质特征等方面的研究进展;剖析了当前产业发展中存在的关键问题,并对未来发展趋势作出展望,旨在为云南大叶种白茶产业的持续健康发展提供理论参考。 展开更多
关键词 白茶 云南大叶种 品质特征 加工工艺
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酸枣叶茶黄酮提取工艺优化及其体外抗氧化、降血糖、降血脂作用 认领 引用
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作者 武丽娜 武延生 +3 位作者 牛伟涛 李敏 刘静雯 王丽婷 《食品研究与开发》 CAS 2026年第5期109-117,共9页
采用单因素试验结合响应面法优化酸枣叶茶黄酮的提取工艺,并研究酸枣叶茶黄酮的体外抗氧化活性、降血糖及降血脂作用。结果表明,酸枣叶茶黄酮提取的最佳工艺条件为超声时间50 min、超声温度65℃、料液比1∶35(g/mL)、乙醇含量61.2%,该... 采用单因素试验结合响应面法优化酸枣叶茶黄酮的提取工艺,并研究酸枣叶茶黄酮的体外抗氧化活性、降血糖及降血脂作用。结果表明,酸枣叶茶黄酮提取的最佳工艺条件为超声时间50 min、超声温度65℃、料液比1∶35(g/mL)、乙醇含量61.2%,该条件下酸枣叶茶黄酮得率为7.39%。在酸枣叶茶黄酮浓度为3.2 mg/mL时,其总抗氧化能力为13.44 U/mL,DPPH自由基清除率达到90.12%。体外降血糖、降血脂试验结果表明,在酸枣叶茶黄酮浓度为3.2 mg/mL时,其对α‑葡萄糖苷酶和α‑淀粉酶的抑制率分别为96.41%和79.40%;对胆酸钠和牛磺胆酸钠的吸附率分别为62.62%和71.79%,对胆固醇的抑制率为79.58%。研究结果表明酸枣叶茶黄酮具有较强的抗氧化活性和一定的体外降血糖、降血脂功效。 展开更多
关键词 酸枣叶茶 黄酮 抗氧化活性 降血糖 降血脂
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Capacity of Tea Scavenging Singlet Oxygen Studied by Means of 1,3-Diphenylisobenzofuran as Fluorescence Probe 认领 引用
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作者 LU Qing-luan YUE Ning-ning +2 位作者 ZHANG Miao GONG Bin WANG Huai-you 《Chemical Research in Chinese Universities》 SCIE CAS 2009年第6期966-970,共5页
In recent years,the effects of reactive oxygen species(ROS)generated in the course of biological metabolism,such as superoxide(O2),hydrogen peroxide(H202),hydroxyl radical(HO)and singlet oxygen(102)on the human health... In recent years,the effects of reactive oxygen species(ROS)generated in the course of biological metabolism,such as superoxide(O2),hydrogen peroxide(H202),hydroxyl radical(HO)and singlet oxygen(102)on the human health have received more attention due to their vital roles in physiological functions.Normally,antioxidant molecules,superoxide dismutase and catalase in biological organism can scavenge excessive free radicals by a series of chemical reactions to keep the cells in a state of redox homeostasis. 展开更多
关键词 Singlet oxygen 1,3-Diphenylisobenzofuran Methylene blue Tea leaf Fluorescence quenching
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18个大叶茶树杂交资源生化成分及品质特征分析 认领 引用
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作者 邓少春 田易萍 +3 位作者 刘本英 陈春林 赵才美 周玉忠 《河南农业科学》 北大核心 2026年第4期59-68,共10页
为筛选生化成分特异、品质特征优良的大叶茶树杂交资源,对云南18个大叶茶树杂交资源的常规生化成分、儿茶素组分及烘青绿茶、红碎茶的感官品质进行测定和评价,并在此基础上进行生化成分与感官品质的相关性分析。结果表明,18个大叶茶树... 为筛选生化成分特异、品质特征优良的大叶茶树杂交资源,对云南18个大叶茶树杂交资源的常规生化成分、儿茶素组分及烘青绿茶、红碎茶的感官品质进行测定和评价,并在此基础上进行生化成分与感官品质的相关性分析。结果表明,18个大叶茶树杂交资源的水浸出物含量平均为49.78%,茶多酚含量平均为17.37%,氨基酸含量平均为3.48%,咖啡碱含量平均为3.98%,酚氨比平均为5.13;儿茶素总量平均为18.60%,没食子酸含量平均为0.80%,表没食子儿茶素含量平均为2.23%,儿茶素含量平均为1.22%,表没食子基儿茶素没食子酸酯含量平均为10.19%,表儿茶素含量平均为1.19%,表儿茶素没食子酸酯含量平均为3.75%。常规生化成分及儿茶素组分的变异系数为1.43%~95.08%,平均变异系数为22.64%。18个烘青绿茶样感官审评结果中,ZJ01得分最高(91.2分),ZJ08得分最低(86.4分);18个红碎茶样感官审评结果中,ZJ01得分最高(91.7分),ZJ15得分最低(87.2分)。生化成分与感官品质的相关性分析表明,红碎茶感官品质与没食子酸、儿茶素、表儿茶素没食子酸酯含量及烘青绿茶感官品质呈显著或极显著正相关,与其他儿茶素组分及常规生化成分含量相关性较小;烘青绿茶感官品质与儿茶素组分及常规生化成分含量的相关性均较小;酯型儿茶素含量与烘青绿茶和红碎茶感官品质为正相关关系,非酯型儿茶素含量与烘青绿茶和红碎茶感官品质为负相关关系。综上,18个大叶茶树杂交资源均为高水浸出物(>45%)材料,其中3个为高氨基酸(≥4.0%)材料,1个为高咖啡碱(≥5.0%)材料;18个大叶茶树杂交资源中,5个为适制绿茶(酚氨比6.5)材料,ZJ01材料兼具绿茶和红茶双重适制性。 展开更多
关键词 大叶茶树 杂交资源 烘青绿茶 红碎茶 生化成分 品质特征
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