期刊文献+
任意字段
题名或关键词
题名
关键词
摘要
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到1,926篇文章
< 1 2 … 97 >
每页显示 20 50 100
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
显示方式：
Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction 认领 引用 被引量：10
1
作者 Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 1044,China 《World Journal of Gastroenterology》 SCIE CAS 2001年第3期370-375,共6页
AIM: To study persistence and replication of hepatitis C virus (HCV) in patients' peripheral blood mononuclear cells (PBMC) cultured in vitro. METHODS: Epstein Barr virus (EBV) was used to transform the hepatitis ... AIM: To study persistence and replication of hepatitis C virus (HCV) in patients' peripheral blood mononuclear cells (PBMC) cultured in vitro. METHODS: Epstein Barr virus (EBV) was used to transform the hepatitis C virus from a HCV positive patient to permanent lymphoblastoid cell lines (LCL). Positive and negative HCV RNA strands of the cultured cells and growth media were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) each month. Core and NS5 proteins of HCV were further tested using immunohistochemical SP method and in situ RT-PCR. RESULTS: HCV RNA positive strands were consistently detected the cultured cells for one year. The negative-strand RNA in LCL cells and the positive-strand RNA in supernatants were observed intermittently. Immunohistochemical results medicated expression of HCV NS3 and C proteins in LCL cytoplasm mostly. The positive signal of PCR product was dark blue and mainly localized to the LCL cytoplasm. The RT-PCR signal was eliminated by overnight RNase digestion but not DNase digestion. CONCLUSION: HCV may exist and remain functional in a cultured cell line for a long period. 展开更多
关键词 B-Lymphocytes Cells, Cultured Female Hepacivirus development purification Herpesvirus 4, Human Humans Immunohistochemistry In Vitro Polymerase Chain Reaction RNA, Viral Research Support, Non-U.S. Gov't Reverse Transcriptase Polymerase Chain Reaction Transformation, Genetic Viral Core Proteins Viral Nonstructural Proteins Virus Replication
暂未订购 下载PDF
Digital polymerase chain reaction detection of telomerase reverse transcriptase promoter mutations in hepatitis B virus related hepatocellular carcinoma 认领 引用
2
作者 Shuai Shao Yu Xiong +2 位作者 Mo-Wei Kong Yang Yu Chun-Xiang Zhang 《World Journal of Gastrointestinal Oncology》 SCIE 2026年第4期1-14,共14页
This editorial provides an in-depth evaluation of the study by Aizimuaji et al published in the recent issue of the World Journal of Gastrointestinal Oncology,which investigates the prognostic value of the telomerase ... This editorial provides an in-depth evaluation of the study by Aizimuaji et al published in the recent issue of the World Journal of Gastrointestinal Oncology,which investigates the prognostic value of the telomerase reverse transcriptase(TERT)promoter C228T mutation in hepatitis B virus-related hepatocellular carcinoma(HCC).The study highlights the innovative application of optimized digital polymerase chain reaction technology to improve the sensitivity of TERT mutation detection.The results demonstrate that the TERT C228T mutation is significantly associated with reduced overall survival and disease-free survival,identifying it as a potential biomarker for poor prognosis.Additionally,the study successfully developed a nomogram model incorporating TERT mutation status,which significantly enhances the accuracy of postoperative recurrence risk prediction.This editorial further discusses the biological significance of TERT mutations in hepatitis B virus-related HCC,emphasizing their role as an early driver of liver carcinogenesis.While digital polymerase chain reaction offers significant advantages over traditional Sanger sequencing,providing a more precise detection tool for clinical applications,large-scale and multi-center validation is still required for broader clinical adoption.Moreover,the editorial explores the potential of integrating TERT mutation detection with other molecular biomarkers for a more comprehensive approach to personalized treatment,providing valuable insights for future precision therapy in liver cancer.This study not only opens new avenues for individualized treatment and early intervention in HCC but also serves as an important reference for the future development of liver cancer biomarkers. 展开更多
关键词 Telomerase reverse transcriptase promoter mutation Digital polymerase chain reaction Hepatitis B virus Hepatocellular carcinoma Telomerase activation Prognostic biomarker Liquid biopsy
暂未订购 下载PDF
Transplantation of human telomerase reverse transcriptase gene-transfected Schwann cells for repairing spinal cord injury 认领 引用 被引量：3
3
作者 Shu-quan Zhang Min-fei Wu +3 位作者 Jia-bei Liu Ye Li Qing-san Zhu Rui Gu 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第12期2040-2047,共8页
Transfection of the human telomerase reverse transcriptase（h TERT）gene has been shown to increase cell proliferation and enhance tissue repair.In the present study,h TERT was transfected into rat Schwann cells.A rat... Transfection of the human telomerase reverse transcriptase（h TERT）gene has been shown to increase cell proliferation and enhance tissue repair.In the present study,h TERT was transfected into rat Schwann cells.A rat model of acute spinal cord injury was established by the modified free-falling method.Retrovirus PLXSN was injected at the site of spinal cord injury as a vector to mediate h TERT gene-transfected Schwann cells（1×10^（10）/L;10μL）or Schwann cells（1×10^（10）/L;10μL）without h TERT gene transfection.Between 1 and 4 weeks after model establishment,motor function of the lower limb improved in the h TERT-transfected group compared with the group with non-transfected Schwann cells.Terminal deoxynucleotidyl transferase-mediated d UTP nick-end labeling and reverse transcription-polymerase chain reaction results revealed that the number of apoptotic cells,and gene expression of aquaporin 4/9 and matrix metalloproteinase 9/2decreased at the site of injury in both groups;however,the effect improved in the h TERT-transfected group compared with the Schwann cells without h TERT transfection group.Hematoxylin and eosin staining,PKH26 fluorescent labeling,and electrophysiological testing demonstrated that compared with the non-transfected group,spinal cord cavity and motor and sensory evoked potential latencies were reduced,while the number of PKH26-positive cells and the motor and sensory evoked potential amplitude increased at the site of injury in the h TERT-transfected group.These findings suggest that transplantation of h TERT gene-transfected Schwann cells repairs the structure and function of the injured spinal cord. 展开更多
关键词 nerve regeneration spinal cord injury Schwann cells transplantation motor function telomerase reverse transcriptase proliferation modification cells neural regeneration
暂未订购 下载PDF
Identification and Characterization of Reverse Transcriptase Fragments of Long Interspersed Nuclear Elements (LINEs) in the Morus notabilisGenome 认领 引用 被引量：1
4
作者 Bi Ma Youchao Xin +2 位作者 Lulu Kuang Fei Hou Ningjia He 《American Journal of Molecular Biology》 CAS 2017年第3期138-152,共15页
Reverse transcriptase (rt) fragments from LINE retrotransposons in the mulberry genome were analyzed in terms of heterogeneity, phylogeny, and chromosomal distribution. We amplified and characterized conserved domains... Reverse transcriptase (rt) fragments from LINE retrotransposons in the mulberry genome were analyzed in terms of heterogeneity, phylogeny, and chromosomal distribution. We amplified and characterized conserved domains of the rt using degenerate primer pairs. Sequence analyses indicated that the rt fragments were highly heterogeneous and rich in A/T bases. The sequence identity ranged from 31.8% to 99.4%. Based on sequence similarities, the rt fragments were categorized into eight groups. Furthermore, similar stop codon distribution patterns among a series of clones in the same group indicated that they underwent a similar evolutionary process. Interestingly, phylogenetic analyses of the rt fragments isolated from mulberry and 13 other plant species revealed that two distantly related taxa (mulberry and Paeonia suffruticosa) grouped together. It does not appear that this phenomenon resulted from horizontal transposable element transfer. Fluorescence in situ hybridization analysis revealed that most of the rt fragments were concentrated in the subtelomeric and pericentromeric regions of the mulberry chromosomes, but that these elements were not abundant in the mulberry genome. Future studies will focus on the potential roles of these elements in the subtelomeric and pericentromeric regions of the mulberry genome. 展开更多
关键词 iMorus notabilis LINE Retrotransposons Reverse Transcriptase Characterization Fluorescence in site Hybridization
暂未订购 下载PDF
Naturally occurring hepatitis B virus reverse transcriptase mutations related to potential antiviral drug resistance and liver disease progression 认领 引用 被引量：19
5
作者 Yu-Min Choi So-Young Lee Bum-Joon Kim 《World Journal of Gastroenterology》 SCIE CAS 2018年第16期1708-1724,共17页
The annual number of deaths caused by hepatitis B virus(HBV)-related disease, including cirrhosis and hepatocellular carcinoma(HCC), is estimated as 887000. The reported prevalence of HBV reverse transcriptase(RT) mut... The annual number of deaths caused by hepatitis B virus(HBV)-related disease, including cirrhosis and hepatocellular carcinoma(HCC), is estimated as 887000. The reported prevalence of HBV reverse transcriptase(RT) mutation prior to treatment is varied and the impact of preexisting mutations on the treatment of na?ve patients remains controversial, and primarily depends on geographic factors, HBV genotypes, HBe Ag serostatus, HBV viral loads, disease progression, intergenotypic recombination and co-infection with HIV. Different sensitivity of detection methodology used could also affect their prevalence results. Several genotype-dependent HBV RT positions that can affect the emergence of drug resistance have also been reported. Eight mutations in RT(rtL80I, rtD134N, rtN139K/T/H, rtY141F, rtM204I/V, rtF221Y, rtI224V, and rtM309K) are significantly associated with HCC progression. HBe Ag-negative status, low viral load, and genotype C infection are significantly related to a higher frequency and prevalence of preexisting RT mutations. Preexisting mutations are most frequently found in the A-B interdomain of RT which overlaps with the HBs Ag "a" determinant region, mutations of which can lead to simultaneous viral immune escape. In conclusion, the presence of baseline RT mutations can affect drug treatment outcomes and disease progression in HBVinfected populations via modulation of viral fitness and host-immune responses. 展开更多
关键词 polymerase hepatocellular carcinoma reverse transcriptase preexisting mutations hepatitis B virus
暂未订购 下载PDF
Unique case of oligoastrocytoma with recurrence and grade progression:Exhibiting differential expression of high mobility group-A1 and human telomerase reverse transcriptase 认领 引用 被引量：3
6
作者 Puneet Gandhi Richa Khare +3 位作者 Kavita Niraj Nitin Garg Sandeep K Sorte Hanni Gulwani 《World Journal of Clinical Cases》 SCIE 2016年第9期296-301,共6页
Mixed gliomas, primarily oligoastrocytomas, account for about 5%-10% of all gliomas. Distinguishing oligoastrocytoma based on histological features alone has limitations in predicting the exact biological behavior, ne... Mixed gliomas, primarily oligoastrocytomas, account for about 5%-10% of all gliomas. Distinguishing oligoastrocytoma based on histological features alone has limitations in predicting the exact biological behavior, necessitating ancillary markers for greater specificity. In this case report, human telomerase reverse transcriptase(hT ERT) and high mobility group-A1(HMGA1); markers of proliferation and stemness, have been quantitatively analyzed in formalin-fixed paraffin-embedded tissue samples of a 34 years old patient with oligoastrocytoma. Customized florescence-based immunohistochemistry protocol with enhanced sensitivity and specificity is used in the study. The patient presented with a history of generalized seizures and his magnetic resonance imaging scans revealed infiltrative ill-defined mass lesion with calcified foci within the left frontal white matter, suggestive of glioma. He was surgically treated at our center for four consecutive clinical events. Histopathologically, the tumor was identified as oligoastrocytoma-grade Ⅱ followed by two recurrence events and final progression to grade Ⅲ. Overall survival of the patient without adjuvant therapy was more than 9 years. Glial fibrillary acidic protein, p53, Ki-67, nuclear atypia index, pre-operative neutrophillymphocyte ratio, are the other parameters assessed. Findings suggest that hT ERT and HMGA1 are linked to tumor recurrence and progression. Established markers can assist in defining precise histopathological grade in conjuction with conventional markers in clinical setup. 展开更多
关键词 Human telomerase reverse transcriptase High mobility group-A1 Oligoastrocytoma Recurrence Tumor grade
暂未订购 下载PDF
SYBR Green I-based product-enhanced reverse transcriptase assay for quantification of retroviral PFV and detection of the divalent cation preference of PFV RT 认领 引用
7
作者 Xiaofang Yi Yan Sun +5 位作者 Qingmei Liu Zhi Li Wanhong Liu Xiaohua He Linjuan Zhang Yuxin Wang 《Virologica Sinica》 CAS CSCD 2014年第5期314-317,共4页
Dear Editor,Prototype foamy virus(PFV)belongs to the genus Spumavirus in the Spumaretrovirinae subfamily of Retroviridae.Although PFV and HIV have much in common,research into PFV has lagged far behind that into HIV,a... Dear Editor,Prototype foamy virus(PFV)belongs to the genus Spumavirus in the Spumaretrovirinae subfamily of Retroviridae.Although PFV and HIV have much in common,research into PFV has lagged far behind that into HIV,as PFV appeared to be non-pathogenic both in accidentally infected humans and in experimentally infected animals.In recent decades,however,more attention has been focused on PFV because it seems to be 展开更多
关键词 preference transcriptase subfamily pathogenic quantification divalent belongs behind infected template
暂未订购 下载PDF
Enhancing thermostability of Moloney murine leukemia virus reverse transcriptase through greedy combination of multiple mutant residues 认领 引用
8
作者 Youhui Yang Jie Zhang +1 位作者 Zhong Li Hao Qi 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期156-168,共13页
Reverse transcription is crucial in bioengineering and biomedical fields,particularly for genome sequencing and virus diagnosis.Enhancing the thermostability of reverse transcriptase can significantly improve its effi... Reverse transcription is crucial in bioengineering and biomedical fields,particularly for genome sequencing and virus diagnosis.Enhancing the thermostability of reverse transcriptase can significantly improve its efficiency and accuracy by enabling it to function at higher temperatures,thereby reducing RNA secondary structures and minimizing interference from contaminating enzymes,particularly in clinical samples.Here,using a combinatorial strategy,a variant of Moloney Murine Leukemia Virus reverse transcriptase(MMLV RT)with improved activity across a wide temperature range(30-50℃)was identified and maintained 100%activity after incubation at 50℃ for 10 min.Eleven hot-spot residues were mutated in various combinations,and the mutant proteins were rapidly expressed in a cell-free system for reverse transcription activity testing.Variant M5,which carries five mutated residues(E47K/E280R/T284R/L413G/D631V),exhibited enhanced thermostability and activity compared to any variant with a single residue mutation.Using purified recombinant protein for precise characterization,the melting temperature(Tm)of M5 increased by 4.7℃ when assembled with a nucleotide template-primer(T/P).Consequently,the half-life of M5 at 50℃ extended to approximately 60 min,in contrast to less than 4 min for the wild type.These findings demonstrate that the epistasis of combining multiple mutant residues holds excellent potential for significantly enhancing enzyme activity,even with existing knowledge.This heat-stable MMLV RT variant M5 may potentially improve efficiency and accuracy in molecular biology research and clinical diagnostics. 展开更多
关键词 Reverse transcriptase Protein engineering Thermostability Melting temperature
暂未订购 下载PDF
Expression of Telomerase Reverse Transcriptase during the Malignant Transformation of Cadmium-Induced Cells 认领 引用
9
作者 Min Wang Yixiong Lei 《Journal of Geoscience and Environment Protection》 2014年第2期129-133,共5页
The objective of the present study was to investigate human telomerase reverse transcriptase (hTERT) mRNA and protein expressions during the cadmium chloride-induced malignant transformation of human bronchial epithel... The objective of the present study was to investigate human telomerase reverse transcriptase (hTERT) mRNA and protein expressions during the cadmium chloride-induced malignant transformation of human bronchial epithelial (16HBE) cells. Fluorescence quantitative PCR (FQ-PCR) and Western blot analyses were performed to detect the hTERT mRNA and protein expressions in normal 16HBE cells, cadmium chloride-transformed 16HBE cells, and tumorigenic cells from nude mice inoculated with cadmium chloride-transformed 16HBE cells. Under the inner standard of GAPDH, the hTERT mRNA expression was significantly higher at different stages of malignant transformation (cadmium chloride-transformed 16HBE cells at passages 15 and 35 and tumorigenic cells from nude mice) than in normal 16HBE cells, and increased with the development of malignancy (P < 0.01). In addition, hTERT protein expression increased with the development of malignancy. These findings demonstrate that hTERT expression is related to cadmium chlorideinduced malignant transformation. Cadmium chloride-induced malignant transformation is involved in changes in the hTERT activity, and might be an early event in cadmium chloride-induced malignant transformation. 展开更多
关键词 Cadmium Chloride Human Bronchial Epithelial Cells Malignant Transformation Telomerase Reverse Transcriptase
暂未订购 下载PDF
Neuroprotective effects of human telomerase reverse transcriptase on beta-amyloid fragment 25-35-treated human embryonic cortical neurons 认领 引用 被引量：3
10
作者 Lingping Kong Lingzhi Wu +2 位作者 Jie Zhang Yaping Liao Huaqiao Wang 《Neural Regeneration Research》 SCIE CAS 2009年第6期405-412,共8页
BACKGROUND： Numerous current studies have suggested that human telomerase reverse transcriptase （hTERT） gene has neuroprotective effects and can inhibit apoptosis induced by various cytotoxic stresses; however, the... BACKGROUND： Numerous current studies have suggested that human telomerase reverse transcriptase （hTERT） gene has neuroprotective effects and can inhibit apoptosis induced by various cytotoxic stresses; however, the mechanism of action remains unknown. OBJECTIVE： To evaluate the neuroprotective effects and possible mechanism of action of hTERT gene transfection in human embryonic cortical neurons treated with beta-amyloid fragment 25-35 （AI325-35）. DESIGN, TIME AND SETTING： The randomized, controlled and molecular biological studies were performed at the Department of Anatomy and Brain Research, Zhongshan School of Medicine, Sun Yat-sen University, China, from September 2005 to June 2008. MATERIALS： AdEasy-1 Expression System was gifted by Professor Guoquan Gao from Sun Yat-Sen University, China. Human cortical neurons were derived from 12-20 week old aborted fetuses, obtained from the Guangzhou Maternal and Child Health Hospital, China. Mouse anti-Odk5 and mouse anti-p16 monoclonal antibodies （Lab Vision, USA）, and mouse anti-hTERT monoclonal antibody （Epitomics, USA）, were used in this study. METHODS： （1） Recombinant adenovirus vectors, encoding hTERT （Ad-hTERT） and green fluorescent protein （Ad-GFP）, were constructed using the AdEasy-1 Expression System. Human embryonic cortical neurons in the Ad-hTERT group were transfected with Ad-hTERT for 1-21 days. Likewise, human embryonic cortical neurons in the Ad-GFP group were transfected with Ad-GFP for 1-21 days. Human embryonic cortical neurons in the control group were cultured as normal. （2） Human embryonic cortical neurons in the Ad-hTERT group were treated with 10 pmol/L Aβ25-35 for 24 hours. Normal human embryonic cortical neurons treated with 10 pmol/Lβ25.35 for 24 hours served as a model group. Human embryonic cortical neurons in the Ad-GFP and control groups were not treated with Aβ25-35. MAIN OUTCOME MEASURES： Expression of hTERT in human embryonic cortical neurons was evaluated by immunocytochemical staining and Western blot assay. Telomerase activity was measured using a PCR-based telomeric repeat amplification protocol （TRAP） ELISA kit. Neural activity in human embryonic cortical neurons was examined by MTT assay; apoptosis was measured using TUNEL assay; and Cdk5 and p16 protein expressions were measured by Western blot. RESULTS： Expression of hTERT protein was significantly increased and peaked at day 3 post-transfection in the Ad-hTERT group. No hTERT expression was detected in the Ad-GFP and control groups. Telomerase activity was significantly greater in the Ad-hTERT group compared with the Ad-GFP and control groups （P 〈 0.01）. Compared with the control group, cell activity was significantly decreased （P 〈 0.05）, and cell apoptotic rate, Cdk5 and p16 expression were significantly increased （P 〈 0.01） in the model group. Compared with the model group, cell activity was increased in the Ad-hTERT group, and peaked at day 3 post-transfection （P 〈 0.05）. Neuroprotective effects also peaked at day 3 post-transfection; and the apoptotic rate, Cdk5 and p16 expression significantly decreased （P 〈 0.01）. CONCLUSION： Expression of hTERT in human embryonic cortical neurons can relieve Aβ25-35-induced neuronal apoptosis. The possible mechanism by which hTERT produces these neuroprotective effects may be associated with inhibition of Cdk5 and p16 expression. 展开更多
关键词 human telomerase reverse transcriptase cortical neuron human embryo Alzheimer＇s disease beta-amyloid fragment 25-35 Cdk5 p16
暂未订购 下载PDF
Covariation of mutation pairs expressed in HIV-1 protease and reverse transcriptase genes subjected to varying treatments 认领 引用
11
作者 David King Roger Cherry Wei Hu 《Journal of Biomedical Science and Engineering》 2010年第3期291-299,共9页
A previous study, focused on the correlation of muta-tion pairs of synonymous (S) and asynonymous (A) mutations, distinguished only between the treated and untreated data of protease and reverse tran-scriptase (RT) of... A previous study, focused on the correlation of muta-tion pairs of synonymous (S) and asynonymous (A) mutations, distinguished only between the treated and untreated data of protease and reverse tran-scriptase (RT) of HIV-1 subtype B. It is well known that single mutation patterns in HIV-1 are treat-ment-specific. It logically follows that covariation between mutations will also be treatment specific. Thus, our motivation is to give a more in depth study of the covariation between mutation pairs, analyzing not only treated and untreated, but what specific treatments were used, and how they affected the co-variation between the mutations differently. We in-tended to further deepen this study by analyzing the covariation of mutations in protease and RT in dif-ferent subtypes of HIV-1. We found that virus sam-ples subjected to antiretroviral Protease- and RT- inhibitors do show different patterns of mutation covariation in B-subtype protease and RT of HIV-1, while maintaining the same overall trend. covariation will tend to be higher and more distinct from and covariation after treatment. The same trend continues in protease and RT re-gardless of subtype. We also found the highly cova-ried codon positions, position pairs, and position- covariation clusters in protease, affected by different treatments. Most of them are well known major drug-resistance sites for these treatments. 展开更多
关键词 HIV Covariation Synonymous Mutation Asynonymous Mutation Protease Reverse Transcriptase Drug Resistance
暂未订购 下载PDF
Testicular expression of survivin and human telomerase reverse transcriptase(hTERT)associated with spermatogenic function in infertile patients 认领 引用 被引量：8
12
作者 Steffen Weikert Frank Christoph +5 位作者 Wolfgang Schulze Hans Krause Carsten Kempkensteffen Martin Schostak Kurt Miller Mark Schrader 《Asian Journal of Andrology》 SCIE CAS 2006年第1期95-100,共6页
Aim： To characterize the coexpression of survivin, an inhibitor of apoptosis （IAF）, and human telomerase reverse transcriptase （hTERT） in human testes with varying spermatogenic function. Methods： Transcript lev... Aim： To characterize the coexpression of survivin, an inhibitor of apoptosis （IAF）, and human telomerase reverse transcriptase （hTERT） in human testes with varying spermatogenic function. Methods： Transcript levels of survivin mRNA and hTERT mRNA were determined in normal testes （n = 11） and testes with defective spermatogenesis （n = 28） using real-time reverse-transcription polymerase chain reaction （RT-PCR）. The histological work-up was performed according to a modified Johnsen score. Results： Expressions of both survivin and hTERT were highest at median levels of 96.8 and 709 in normal spermatogenesis and dropped to 53.3 and 534 in testes with postmeiotic spermatogenic arrest （n = 10）. In severe spermatogenic failure （n = 18）, survivin expression was lacking in most specimens （n = 16）, whereas at least low levels of testicular hTERT expression were largely detectable with a normalized expression of 73 in premeiotic spermatogenic arrest （n = 7） and 45 in patients with Sertoli cell-only syndrome （SCOS） （n = 3）. Both survivin and hTERT expressions increased with a progressing Johnsen score （P for trend = 0.001）. Conclusion： Although both survivin and hTERT are correlated with spermatogenic function, they show different expression patterns in testes of infertile patients. These findings substantiate results from studies in the rodent testis suggesting a predominant expression of survivin in meiotically dividing germ cells. （Asian J Andro12006 Jan; 8： 95-100） 展开更多
关键词 survivin human telomerase reverse transcriptase apoptosis azoospermia male infertility spermatogenesis
暂未订购 下载PDF
Naturally occurring mutations in the reverse transcriptase region of hepatitis B virus polymerase from treatment-na?ve Korean patients infected with genotype C2 认领 引用 被引量：4
13
作者 Ji-Eun Kim So-Young Lee +3 位作者 Hong Kim Ki-Jeong Kim Won-Hyeok Choe Bum-Joon Kim 《World Journal of Gastroenterology》 SCIE CAS 2017年第23期4222-4232,共11页
AIM To report naturally occurring mutations in the reverse transcriptase region(RT)of hepatitis B virus(HBV)polymerase from treatment na?ve Korean chronic patients infected with genotype C2.METHODS Here,full-length HB... AIM To report naturally occurring mutations in the reverse transcriptase region(RT)of hepatitis B virus(HBV)polymerase from treatment na?ve Korean chronic patients infected with genotype C2.METHODS Here,full-length HBV reverse transcriptase RT sequences were amplified and sequenced from 131 treatment na?ve Korean patients chronically infected with hepatitis B genotype C2.The patients had two distinct clinical statuses:59 patients with chronic hepatitis(CH)and 72 patients with hepatocellular carcinoma(HCC).The deduced amino acids(AAs)at42 previously reported potential nucleos(t)ide analog resistance(NAr)mutation positions in the RT region were analyzed.RESULTS Potential NAr mutations involving 24 positions were found in 79 of the 131 patients(60.3%).Notably,AA substitutions at 2 positions(rt184 and rt204)involved in primary drug resistance and at 2 positions(rt80 and rt180)that functioned as secondary/compensatory mutations were detected in 10 patients(1 CH patient and 9 HCC patients)and 7 patients(1 CH and 6 HCC patients),respectively.The overall mutation frequencies in the HCC patients(3.17%,96/3024 mutations)were significantly higher than the frequencies in the CH patients(2.09%,52/2478 mutations)(P=0.003).In addition,a total of 3 NAr positions,rt80,rt139 and rt204 were found to be significantly related to HCC from treatment na?ve Korean patients.CONCLUSION Our data showed that naturally occurring NAr mutations in South Korea might contribute to liver disease progression(particularly HCC generation)in chronic patients with genotype C2 infections. 展开更多
关键词 Hepatitis B virus Polymerase Reverse transcriptase Potential nucleos(t)ide analog resistance Chronic hepatitis Hepatocellular carcinoma
暂未订购 下载PDF
Rapid screening of anti--HIV ingredients in Artemisia rupestris L. extracts interacting with V3 loop region of HIV--1 gp120 and reverse transcriptase by affinity capillary electrophoresis and capillary zone electrophoresis 认领 引用
14
作者 赵怡然 李中杰 +2 位作者 姜勇 张晓丹 凌笑梅 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2015年第9期625-629,共5页
HIV-1 gains entry into target cells by sequentially interacting with cellular receptors and co-receptors. Both the receptor and co-receptor are recognized by HIV-1 envelope protein gpl20, which plays a key role in the... HIV-1 gains entry into target cells by sequentially interacting with cellular receptors and co-receptors. Both the receptor and co-receptor are recognized by HIV-1 envelope protein gpl20, which plays a key role in the entry process of HIV-1 into cells. The development of new inhibitors is essential since the viral enzyme reverse transcriptase （RT） is one of the first targets of antiretroviral therapy. It has been reported that a variety of natural plants, such as Artemisia rupestris L., have anti-viral pharmacological activity, and they might be the potential inhibitors of RT or V3 loop of gpl20 against HIV-1. RIQRGPGRAFVT1GK （R15K）, the relatively conserved region of V3 loop, can be used for binding research. In this work, we analyzed the interactions between different extracts from Artemisia rupestris L. and R15K by affinity capillary electrophoresis （ACE）. Moreover, we analyzed the interactions between different extracts from Artemisia rupestris L. and RT by capillary zone electrophoresis （CZE）. Our data showed that the chloroform extract ofArtemisia rupestris L. was active among the different plant extracts, which was consistent with previous studies. Taken together, our study provided a rapid screening method to seek anti-HIV ingredients in natural plants＇ extracts. 展开更多
关键词 HIV V3 loop RIQRGPGRAFVTIGK Reverse transcriptase Affinity capillary electropiaoresis Capillary zone electrophoresis
暂未订购 下载PDF
Upreguiation of human telomerase reverse transcriptase mRNA expression by in vitro transfection of hepatitis B virus X gene into human hepatocarcinoma and cholangiocarcinoma cells 认领 引用 被引量：21
15
作者 Zhen-Liang Qu Sheng-Quan Zou +4 位作者 Nai-Qiang Cui Xian-Zhong Wu Ming-Fang Qin Di Kong Zhen-Li Zhou 《World Journal of Gastroenterology》 SCIE CAS 2005年第36期5627-5632,共6页
AIM： To study the changes of human telomerase reverse transcriptase （hTERT） mRNA expression in human hepatocarcinoma cell lines （HepG2） and cholangiocarcinoma cell lines （QBC939） after HBx gene transfection and... AIM： To study the changes of human telomerase reverse transcriptase （hTERT） mRNA expression in human hepatocarcinoma cell lines （HepG2） and cholangiocarcinoma cell lines （QBC939） after HBx gene transfection and to illustrate the significance of transcriptional regulation of hTERT gene by HBx gene in the carcinogenesis. METHODS： HepG2 and QBC939 cell lines were cultured and co-transfected with eukaryotic expression vector containing the HBx coding region and cloning vector containing enhanced green fluorescent protein （EGFP） coding sequence using lipid-mediated gene transduction technique. Thirty-six hours after transfection, EGFP expression in cells was used as the indicator of successful transfection. Flow cytometry was performed to determine the transfection efficiency. Cells were harvested and total RNA was extracted using TRIzol reagent. The expression of hTERT mRNA in HepG2 and QBC939 cell lines was assayed by reverse transcriptionpolymerase chain reaction. The expression of HBx protein in both cell lines was detected by immunocytochemical staining and Western blotting. RESULTS： Flow cytometry showed that the transfection efficiency was 46.4% in HepG2 cells and 29.6% in QBC939 cells for both HBx gene expression vector and blank vector. The expression of hTERT mRNA was meaningfully increased in HepG2 and QBC939 cell lines when transfected with HBx gene expression vector compared to those transfected with OPTI-MEM medium and blank vector. Immunocytochemical staining and Western blotting revealed HBx protein expression in HepG2 and QBC939 cells only when transfected with HBx gene. CONCLUSION： HBx gene transfection can upregulate the transcriptional expression of hTERT mRNA. The transactivation of hTERT gene by HBx gene is a newfound mechanism for pathogenesis of hepatocarcinomas and cholangiocarcinomas after HBV infection. 2005 The WJG Press and Elsevier Inc. All rights reserved 展开更多
关键词 Hepatocholangiocarcinoma Human telomerasereverse transcriptase Gene expression Hepatitis B virus X protein
暂未订购 下载PDF
Telomerase Activity and Telomerase Reverse Transcriptase Expression Induced by Selenium in Rat Hepatocytes 认领 引用 被引量：2
16
作者 RI-AN YU HUA-JIE CHEN +2 位作者 LING-FEI HE BING CHEN XUE-MIN CHEN 《Biomedical and Environmental Sciences》 SCIE CAS 2009年第4期311-317,共7页
Objective To investigate the effects of sodium selenite on telomerase activity, apoptosis and expression of TERT, c-myc and p53 in rat hepatocytes. Methods Selenium at doses of 2.5, 5.0, and 10 μmol/kg was given to S... Objective To investigate the effects of sodium selenite on telomerase activity, apoptosis and expression of TERT, c-myc and p53 in rat hepatocytes. Methods Selenium at doses of 2.5, 5.0, and 10 μmol/kg was given to SD rats by garage. In rat hepatocytes, telomerase activity was measured by the telomeric repeat amplification protocol （TRAP）, apoptosis was detected by flow cytometry, and expressions of telomerase reverse transcriptase （TERT）, c-myc and p53 were analyzed by reverse transcription-polymerase chain reaction （RT-PCR）. c-Myc and P53 proteins were detected by immunochemistry. Results Selenium at doses of 2.5, 5.0, and 10 μmol/kg significantly increased hepatocellular telomerase activity and induced apoptosis in a dose-dependent manner. Although selenium at doses of 2.5, 5.0, and 10 μmol/kg displayed no obvious enhancing effect on the TERT mRNA expression in rat hepatocytes （P〉0.05）, it significantly increased the c-myc mRNA and p53 mRNA expression at the dose of 10 μmol/kg （P〈0.05）. Selenium at doses of 5.0 and 10 μmol/kg obviously increased the content of P53 protein in rat hepatocytes, but only at the dose of 10 μmol/kg, it significantly promoted the value of c-Myc protein in them. Conclusion Selenium can slightly increase telomerase activity and TERT expression, and significantly induce apoptosis and over-expression of c-myc and p53 at relatively high doses. The beneficial effects of selenium on senescence and aging may be mediated by telomerase activation and expression of TERT, c-myc, and p53 in rat hepatocytes. 展开更多
关键词 Selenium Telomerase Telomerase reverse transcriptase c-myc p53
暂未订购 下载PDF
Inhibition of telomerase with human telomerase reverse transcriptase antisense increases the sensitivity of tumor necrosis factor-α-induced apoptosis in prostate cancer cells 认领 引用 被引量：3
17
作者 Xiao-Dong Gao Yi-Rong Chen 《Asian Journal of Andrology》 SCIE CAS 2007年第5期697-704,共8页
Aim： To investigate the effect of inhibition of telomerase with human telomerase reverse transcriptase （hTERT） antisense on tumor necrosis factor-α （TNF-α-induced apoptosis in prostate cancer cells （PC3）. Meth... Aim： To investigate the effect of inhibition of telomerase with human telomerase reverse transcriptase （hTERT） antisense on tumor necrosis factor-α （TNF-α-induced apoptosis in prostate cancer cells （PC3）. Methods： Antisense phosphorothioate oligodeoxynucleotide （AS PS-ODN） was synthesized and purified. Telomerase activity was measured using the telomeric repeat amplification protocol （TRAP） and polymerase chain reaction enzyme-linked immunoassay （PCR-ELISA）. hTERT mRNA was measured by reverse transcription PCR （RT-PCR） assay and gel-image system, hTERT protein was detected by immunochemistry and flow cytometry. Cell viability was detected by 3-（4, 5-dimethylthiazol-2-yl）-2,5-Diphenyltetrazolium （MTT） assay. Cell apoptosis was observed by morphological method and determined by flow cytometry. Results： The telomerase activity decreased with time after hTERT AS PS-ODN treatment. The levels of hTERT mRNA decreased with time after hTERT AS PS-ODN treatment, which appeared before the decline of the telomerase activity. The percentage of positive cells of hTERT protein declined with time after hTERT AS PS-ODN treatment, which appeared after the decline of hTERT mRNA. There was no difference in telomerase activity, hTERT mRNA and protein levels between hTERT sense phosphorothioate oligodeoxynucleotide （S PS-ODN） and the control group. The cell viability decreased with time after hTERT AS PS-ODN combined with TNF-α treatment. The percentage of apoptosis increased with time after hTERT AS PS-ODN combined with TNF-α treatment. There was no difference in cell viability and the percentage of apoptosis between hTERT S PS-ODN and the control group. Conclusion： hTERT AS PS-ODN can significantly inhibit telomerase activity by downregulating the hTERT mRNA and protein expression, and inhibition of telomerase with hTERT antisense can enhance TNF-α- induced apoptosis of PC3 cells. 展开更多
关键词 human telomerase reverse transcriptase antisense phosphorothioate oligodeoxynucleotide telomerase prostate cancer cells tumor necrosis factor-α
暂未订购 下载PDF
Molecular Modeling of Quinoline-Based Compounds as Potential Dual Inhibitors of Reverse Transcriptase and Integrase of HIV 认领 引用 被引量：1
18
作者 Alberto Cabrera Leonor Huerta Hernández +1 位作者 Daniel Chávez José L. Medina-Franco 《Computational Molecular Bioscience》 2018年第3期122-148,共27页
As follow-up of our past publication?[1], we propose that quinolones (as part of the pyridinone family) are capable to increase the number of interactions with HIV reverse transcriptase (RT) or integrase (IN) by addin... As follow-up of our past publication?[1], we propose that quinolones (as part of the pyridinone family) are capable to increase the number of interactions with HIV reverse transcriptase (RT) or integrase (IN) by adding a halogen in position C-8 of aromatic portion of the quinolones. This addition could help with the activity of dual inhibitors of RT and IN. In this work, we add a chlorine atom with the rationale to identify in the docking simulations a halogen interaction with the oxygen in the near aminoacids in the binding pockets of RT and IN enzymes. Our docking studies started with RT and 320 structures. Later, we took 73 structures with good results in docking with RT. The structures that we choose contain ester or acids groups in C-3 due the structural similarity with groups in charge to interact with the Mg++ ions in Elvitegravir. In conclusion, we obtained 14 structures that could occupy the allosteric pocket of RT and could inhibit the catalytic activity of IN, for this reason could be dual inhibitors. A major perspective of this work is the synthesis and testing of the potential dual inhibitors designed. 展开更多
关键词 Reverse Transcriptase Integrase Quinolone Dual Inhibitor Docking
暂未订购 下载PDF
Isolation and Characterization of Ty1/Copia-like Reverse Transcriptase Sequences from Mung Bean 认领 引用 被引量：1
19
作者 XIAO Wei-Min SAKAMOTO Wataru Sodmergen 《Acta Botanica Sinica》 2004年第5期582-587,共6页
Ty1/copia-like sequences were amplified from mung bean(Vigna radiata(L.)Wilczek)genomic DNA,by PCR with degenerate oligonucleotide primers corresponding to highly conserved domains in the Ty1/copia-like retrotransposo... Ty1/copia-like sequences were amplified from mung bean(Vigna radiata(L.)Wilczek)genomic DNA,by PCR with degenerate oligonucleotide primers corresponding to highly conserved domains in the Ty1/copia-like retrotransposons.PCR fragments of roughly 270 bp were isolated and cloned,and forty clones were sequenced.Thirty-six of the forty clones had unique nucleotide sequences,and eighteen clones had a frameshift,a stop codon,or both.Alignment of the nucleotide sequences indicated that these clones,denoted Tvr,fell into nine subgroups and nine ungrouped sequences.The nucleotide sequence similarity between these elements ranged from 8%to 100%,which indicates high level of sequence heterogeneity among these clones.A phylogenetic analysis comparing these clones with corresponding sequences from other plant species showed that some of the Tvr clones are more closely related to Ty1/copia-like retrotransposons from other species than to other Tvr clones.Dot blot analysis revealed that Ty1/copia-like retrotransposons comprise about 9.3%of the mung bean genome. 展开更多
关键词 mung bean DOP-PCR copia retrotransposon reverse transcriptase
暂未订购 下载PDF
Structural biology of HIV-1 reverse transcriptase allosteric inhibitors for drug design 认领 引用
20
作者 Zhenzhen Zhou Yanying Sun +6 位作者 Da Feng Zhao Wang Fabao Zhao Shenghua Gao Peng Zhan Dongwei Kang Xinyong Liu 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2026年第1期93-121,共29页
HIV-1 reverse transcriptase(RT)is responsible for reverse transcription of viral single-stranded RNA to double-stranded DNA,which plays an important role in the replication cycle of HIV-1 and has been identified as a ... HIV-1 reverse transcriptase(RT)is responsible for reverse transcription of viral single-stranded RNA to double-stranded DNA,which plays an important role in the replication cycle of HIV-1 and has been identified as a key target for anti-HIV-1 drug discovery.Among HIV-1 RT inhibitors,allosteric inhibitors acting on non-catalytic sites have the advantages of high efficiency and low cytotoxicity,which are the focus of the research on anti-HIV-1 inhibitors.Great progress has been achieved in the structural biology of HIV-1 RT,which significantly facilitated the development of RT allosteric inhibitors.Herein,we provided a detailed review of the co-crystal structures of small molecule allosteric inhibitors in complex with RT reported in the last decade.Moreover,the strategies to discover novel and efficient inhibitors based on co-crystal structures have also been discussed,expecting to provide a reference for the development of the next-generation anti-HIV-1 drugs. 展开更多
关键词 HIV-1 Reverse transcriptase Anti-HIV-1 drugs NNRTIs Structural biology Allosteric inhibitors Drug design Drug resistance
暂未订购 下载PDF
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
上一页 1 2 … 97 下一页 到第 页
在线咨询 使用帮助 返回顶部 意见反馈