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Translocalization and Social Rescaling:Case Studies of Linguistic Landscapes in Guangzhou 认领 引用 被引量：3
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作者 Yanmei HAN Xiaodan Wu 《Chinese Journal of Applied Linguistics》 2020年第1期26-44,126,共19页
Languages and linguistic resources transport from one locality to another,adapting to the norms,customs,and regulations of a new locality.This process involves translocalization.Translocalization emphasizes the moveme... Languages and linguistic resources transport from one locality to another,adapting to the norms,customs,and regulations of a new locality.This process involves translocalization.Translocalization emphasizes the movement of linguistic resources against the backdrop of globalization and the combination or reframing of resources from different localities.This research explores the extent to which translocalization is reflected by the linguistic landscapes of three distinct commercial areas in Guangzhou,China.It goes on to discuss how translocalization works together with social rescaling to incur the movement of linguistic resources and to result in distinct linguistic landscapes of the three commercial areas.It concludes that some languages or linguistic resources,such as English,pinyin and traditional Chinese writing,are transported to local contexts for the purpose of rescaling,whereas other languages or dialects,like Cantonese,might gradually lose their function of rescaling and retain its function in indexing local identity and solidarity.This study calls for more attention to the local resources and contexts in linguistic landscape studies.It argues for the indexical function of linguistic resources in social rescaling and city planning. 展开更多
关键词 linguistic landscape translocalization social rescaling multilingualism Mandarin Cantonese
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Integration of multi-omics analyses reveals molecular mechanisms of L-phenylalanine-induced root to shoot cadmium translocation in Kentucky bluegrass 认领 引用
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作者 Ting Cui Yong Wang +2 位作者 Kuiju Niu Chunxu Zhao Huiling Ma 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第8期1938-1953,共16页
Exogenous l-phenylalanine application enhances cadmium(Cd)uptake and translocation in Kentucky bluegrass(Poa pratensis L.),offering a potential strategy for the phytoremediation of Cd-contaminated soils.This study inv... Exogenous l-phenylalanine application enhances cadmium(Cd)uptake and translocation in Kentucky bluegrass(Poa pratensis L.),offering a potential strategy for the phytoremediation of Cd-contaminated soils.This study investigated the role of exogenous l-phenylalanine in Cd uptake,translocation,and detoxification in Kentucky bluegrass.We employ a comprehensive approach combining integrated phenotypic analysis,antioxidant characteristics,Cd content determination,full-length transcriptome sequencing,transcriptomic analysis,proteome,and metabolome analyses to elucidate the molecular mechanisms underlying the long-distance Cd transport promotion by exogenous l-phenylalanine in Kentucky bluegrass,and to validate the function of the candidate gene PpNAS in Arabidopsis thaliana.l-phenylalanine at concentrations of 0.1,1,and 10 mg·L−1induced the Cd content in the leaves by 21%,45%,and 64%.However,the malondialdehyde content was not significantly different under 1 mg·L−1l-phenylalanine and Cd treatment compared to that under Cd treatment alone,but the Cd content significantly increased.In addition,1 mg·L−1l-phenylalanine and Cd treatment improved the antioxidant enzyme activity and biomass of Kentucky bluegrass under Cd stress.l-phenylalanine application upregulated genes associated with Cd uptake(HIPPs),long-distance translocation(HMAs,YSLs,and NAS),and vacuolar compartmentalization pathways(OPTs,ABCCs,and MTPS).Overexpression of PpNAS in Arabidopsis thaliana increased the Cd content in leaves and roots,supporting the positive role of l-phenylalanine in long-distance Cd transport.These results demonstrate that exogenous l-phenylalanine targets multiple metabolic pathways and Cd transporters to regulate Cd redistribution in Kentucky bluegrass,providing new strategies for the phytoremediation of Cd-contaminated soils utilizing l-phenylalanine. 展开更多
关键词 Phytoremediation Cadmium pollution Kentucky bluegrass Long-distance translocation L-phenylalanine
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Development of a Novel 18F-Labeled Radioligand for Imaging Translocator Protein With Positron Emission Tomography 认领 引用
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作者 Jian Rong Chunyu Zhao +6 位作者 Xin Zhou Jiahui Chen Yabiao Gao Zhendong Song Yinlong Li Zijing Li Steven H.Liang 《iRADIOLOGY》 CSCD 2026年第3期261-272,共12页
Background:Translocator protein 18 kDa(TSPO)is a well-established biomarker of neuroinflammation and is implicated in the pathophysiology of various neurodegenerative diseases,including Alzheimer's disease(AD).Des... Background:Translocator protein 18 kDa(TSPO)is a well-established biomarker of neuroinflammation and is implicated in the pathophysiology of various neurodegenerative diseases,including Alzheimer's disease(AD).Despite existing radioligands,there remains a critical need for novel TSPO-targeted positron emission tomography(PET)tracers with improved binding specificity,higher brain uptake,and favorable pharmacokinetics.Methods:We designed and synthesized a class of novel TSPO ligand candidates.Following in vitro binding affinity screening,the lead Compound 3(TSPO-1118)was radiolabeled with fluorine-18.[18F]3([18F]TSPO-1118)was evaluated in two transgenic AD mouse models(3xTg and APP/PS1)and compared to age-matched wild-type(WT)controls.Biodistribution and radiometabolite analyses were conducted in CD-1 mice to assess brain penetration and metabolic stability.Results:Compound 3(TSPO-1118)demonstrated superior binding affinity for TSPO(Ki=2.3 nM),outperforming both other analogs in the series and the benchmark tracer GE180.[18F]3([18F]TSPO-1118)exhibited significantly higher brain uptake in both 3xTg and APP/PS1 mice relative to WT controls,including the cortex,thalamus,cerebellum,and hippocampus(p<0.05 to<0.01,Student's two-tailed t-test),consistent with elevated TSPO expression in disease models.The tracer also displayed favorable brain kinetics,high specific binding,and metabolic stability in vivo.Conclusions:[18F]3([18F]TSPO-1118)represents a promising next-generation PET radioligand for imaging TSPO with high affinity,robust brain uptake,and specificity in preclinical models of neurodegeneration.Its favorable profile supports further translational development toward clinical application in neuroinflammatory and neurodegenerative disorders. 展开更多
关键词 fluorine-18 positron emission tomography translocator protein translocator protein 18 kDa
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Influences of arbuscular mycorrhizal fungi and zinc oxide nanoparticles on cadmium uptake and accumulation in winter wheat grown in naturally cadmium-contaminated soils 认领 引用
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作者 Jing YANG Yuanzhe MA +4 位作者 Chuangye ZHANG Yunmei WU Yifan LIU Huini DU Fuyong WU 《Pedosphere》 SCIE CAS CSCD 2026年第3期669-682,共14页
Cadmium(Cd)contamination in agricultural systems poses a threat to human health.Arbuscular mycorrhizal fungi(AMF)and zinc oxide nanoparticles(ZnO NPs)applied alone have been shown to enhance winter wheat growth in Cd-... Cadmium(Cd)contamination in agricultural systems poses a threat to human health.Arbuscular mycorrhizal fungi(AMF)and zinc oxide nanoparticles(ZnO NPs)applied alone have been shown to enhance winter wheat growth in Cd-contaminated soil,but their combined effects remain largely unexplored,especially in naturally Cd-contaminated soils.Two naturally Cd-contaminated soils with different pH levels,an alkaline soil and an acidic soil collected from Henan and Hunan provinces,respectively,in China,were used to assess the effects of AMF and ZnO NPs on growth and Cd accumulation in winter wheat.The combined application of AMF and ZnO NPs significantly reduced(by 41%-71%)Cd concentration in winter wheat grown in Cd-contaminated soils.Specifically,the treatment with AMF and 20 mg kg−1ZnO NPs effectively decreased soil available Cd by 18%-32%and reduced wheat grain Cd concentration by 60%-71%,ensuring compliance with China's food safety threshold(<0.2 mg kg−1).Additionally,this treatment increased Zn concentration in wheat grain by 17.6%-31.3%,meeting the recommended dietary intake value for Zn(45-60 mg kg−1).Structural equation models(SEM)and correlation analysis showed that the reduction in grain Cd concentration was associated with a significant(P<0.01)decrease in soil available Cd,an increase in soil pH(especially in the acidic soil),restricted Cd translocation due to Zn-Cd competitive antagonism,and“dilution effect”resulting from improved plant growth.Overall,this study demonstrated that the combined application of AMF and low-dose ZnO NPs(20 mg kg−1)was the optimized strategy to reduce grain Cd concentration and improve Zn uptake in winter wheat grown in Cd-contaminated soils,regardless of soil pH. 展开更多
关键词 available Cd Cd contamination Cd fraction Cd translocation grain Cd concentration interactive effect
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Hippocampal Hap1 down-regulation impairs glucocorticoid receptor nuclear translocation and exacerbates Alzheimer’s disease-related neuropathology in APP/PS1 mice 认领 引用
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作者 Taiqi Huang Meiyu Zhang +7 位作者 Yanyu Zhang Chonglin Su Enhao He Jinrong Wang Jiawei Yang Yongxiao Liu Yan Zeng Xingxing Chen 《Zoological Research》 SCIE CSCD 2026年第2期530-546,共17页
Impaired nuclear translocation of glucocorticoid receptor(GR)has been implicated in hippocampal vulnerability in Alzheimer's disease(AD),yet the molecular basis of this defect remains poorly understood.This study ... Impaired nuclear translocation of glucocorticoid receptor(GR)has been implicated in hippocampal vulnerability in Alzheimer's disease(AD),yet the molecular basis of this defect remains poorly understood.This study identified Huntingtin-associated protein 1(Hap1)as a critical regulator of GR nuclear translocation in the hippocampus.Specifically,Hap1 expression progressively declined in the hippocampus of APP/PS1 mice with advancing age and pathological burden.,Hippocampal Hap1 knockdown induced pronounced cognitive deficits and synaptic deterioration,asi indicated by reduced dendritic arborization,decreased spine density,impaired long-term potentiation,,and exacerbated amyloid-βdeposition.Mechanistic中analyses showed that Hap1中deficiency increased GR ubiquitination and proteasomal degradation and,more importantly,disrupted ligand-dependent GR translocation to the nucleus,thereby attenuating GRdependent brain-derived neurotrophic factor transcription.In parallel,Hap1 knockdown elevated corticosterone concentration and induced depression-like behavior,consistent with hypothalamic-pituitary-adrenal axis dysregulation.Collectively,these findings establish defective GR nuclear trafficking driven by loss of Hap1function as a key pathomechanism linking intracellular transport failure to synaptic dysfunction in AD and highlight Hap1 as a potential therapeutic target. 展开更多
关键词 Glucocorticoid receptor Huntingtinassociated protein 1 Alzheimer’s disease Nuclear translocation Synaptic plasticity
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Development and characterization of a new wheat-rye T6BS.6BL-6RLKusmall segment translocation with powdery mildew resistance and potential breeding value 认领 引用
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作者 Zongxiang Tang Ruiyu Zhang +4 位作者 Shuangjun Gong Guangrong Li Zujun Yang Wentao Dong Shulan Fu 《The Crop Journal》 SCIE CSCD 2026年第3期915-922,共8页
Rye(Secale cereale L.)contains numerous disease resistance genes that can be utilized for wheat(Triticum aestivum)improvement.For example,rye chromosome 6 carries powdery mildew resistance genes.Wheat-rye 6R transloca... Rye(Secale cereale L.)contains numerous disease resistance genes that can be utilized for wheat(Triticum aestivum)improvement.For example,rye chromosome 6 carries powdery mildew resistance genes.Wheat-rye 6R translocation lines are highly useful,but more wheat-rye 6R translocation lines with potential breeding value are needed.In this study,we identified a new wheat-rye T6 BS.6 BL-6 RLKutranslocation chromosome,conferring powdery mildew resistance,from the progeny of the irradiated wheat-rye 6 RLKuditelosomic addition line.Genotyping using the wheat GBW16K array and specific markers revealed that approximately 104.03 Mb of the distal segment of 6 RLKureplaced approximately6.1 Mb of the distal segment of the long arm of 6 B(6 BL)to form the translocation chromosome.OligoFISH painting indicated that the 104.03 Mb segment of 6 RLKuis homologous to homoeologous group 7 chromosomes.Using wheat cultivars Chuanmai 62(CM62)and Chuannong 32(CN32)as backcross parents,we transferred the T6 BS.6 BL-6 RLKuchromosome into the two wheat backgrounds.We selected two translocation lines:CM62-6 RL and CN32-6 RL.Genotyping analysis indicated that the CM62-6 RL and CN32-6 RL genomes are highly similar to those of CM62 and CN32,respectively.The grains of CM62-6 RL were shriveled,whereas those of CN32-6 RL were full.The effect of the T6 BS.6 BL-6 RLKuchromosome on grain width also depended on the genetic background of the wheat.The improved grain lengths of both CM62-6 RL and CN32-6 RL contributed to the improvement in their thousand-kernel weight.The translocation chromosome had no negative effects on other important agronomic traits.These findings highlight the potential breeding value of the wheat-rye 6R translocation chromosome T6 BS.6 BL-6 RLKu.The compensation mechanisms of this translocation chromosome in different wheat backgrounds deserve further study. 展开更多
关键词 Wheat Rye Chromosome 6R Powdery mildew Small segment translocation
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Gut barrier dysfunction and multidrug-resistant bacterial translocation in adult critical illness:Mechanistic insights from a systematic review 认领 引用
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作者 Shree V Dhotre Pradnya S Dhotre +1 位作者 Sachin S Mumbre Basavraj S Nagoba 《World Journal of Gastrointestinal Pathophysiology》 2026年第2期47-60,共14页
BACKGROUND The gastrointestinal tract plays an important role in host defence during critical illness.Disruption of epithelial integrity,microbiome imbalance,and immune dysregulation have all been linked to the transl... BACKGROUND The gastrointestinal tract plays an important role in host defence during critical illness.Disruption of epithelial integrity,microbiome imbalance,and immune dysregulation have all been linked to the translocation of multidrug-resistant(MDR)organisms from intestinal colonization to invasive infection.However,whether these associations reflect true causal mechanisms remains uncertain,and available human evidence has not been comprehensively synthesized using current methodological standards.AIM To systematically evaluate human evidence examining the relationship between intestinal barrier dysfunction,microbial colonization,and subsequent MDR infection in adult critical illness,with particular attention to study quality,heterogeneity,and potential confounding factors.METHODS This systematic review was conducted in accordance with PRISMA guidelines.A structured literature search was performed in PubMed,EMBASE,and the Cochrane Library(2000-2025)using predefined Boolean combinations and Medical Subject Headings.Prospective and retrospective cohort studies involving intensive care units(ICU)adults were included if they evaluated intestinal colonization,biomarkers of barrier dysfunction(citrulline and intestinal fatty acid-binding protein),microbiome alterations,or endotoxemia.Study selection and data extraction were undertaken independently by two reviewers,with disagreements resolved through discussion.Risk of bias was assessed using the Newcastle-Ottawa Scale and ROBINS-I tool.Owing to methodological and clinical heterogeneity,findings were synthesized using a structured narrative approach rather than meta-analysis.RESULTS Across the included studies,intestinal colonization with carbapenem-resistant Enterobacteriaceae,carbapenemresistant Klebsiella pneumoniae,Acinetobacter baumannii,and vancomycin-resistant Enterococcus was consistently associated with an increased risk of subsequent bloodstream infection.However,progression rates varied considerably across cohorts,likely reflecting differences in patient characteristics,antimicrobial exposure,and ICU practices rather than a consistent effect size.Biomarker studies showed reduced citrulline levels and elevated intestinal fatty acid-binding protein concentrations in patients with gastrointestinal dysfunction;however,these markers indicate enterocyte injury rather than directly measuring intestinal permeability or bacterial translocation.Microbiome analyses demonstrated reduced diversity and impaired colonization resistance,although the extent and timing of these changes were not uniform across studies.Taken together,the evidence supports a biologically plausible link between epithelial injury,dysbiosis,and infection risk,but does not establish a direct causal relationship,largely due to the observational design of available studies and the influence of confounding factors such as illness severity,antimicrobial exposure,and ICU environment.CONCLUSION Gut barrier dysfunction appears to contribute to the pathogenesis of MDR infection in critically ill adults;however,current evidence supports association rather than causation.Early recognition of intestinal colonization and strategies aimed at preserving mucosal integrity may offer potential clinical benefit,although their effectiveness requires confirmation in well-designed prospective and interventional studies. 展开更多
关键词 Gut barrier dysfunction Tight junctions Multidrug-resistant bacteria Carbapenem-resistant Enterobacteriaceae Bacterial translocation Critical illness Sepsis Gut microbiome dysbiosis
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Ruminal dysbiosis-induced mastitis:new insight into the pathogenesis of mastitis 认领 引用
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作者 Caijun Zhao Xiaochun Sun +3 位作者 Naisheng Zhang Xiaoyu Hu Hongyan Li Yunhe Fu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期42-59,共18页
Mastitis is one of the most significant diseases affecting the development of the dairy industry and has traditionally been associated with pathogenic infections.However,emerging evidence highlights that ruminal micro... Mastitis is one of the most significant diseases affecting the development of the dairy industry and has traditionally been associated with pathogenic infections.However,emerging evidence highlights that ruminal microbial homeostasis also plays a crucial role in the pathogenesis of mastitis.Specifically,cows with mastitis exhibit reduced alpha diversity and altered microbial composition in the rumen.Inducing ruminal dysbiosis through a high-concentrate diet has been shown to trigger mastitis in cows,and transplantation of ruminal microbiota from mastitis-affected cows to recipient mice can induce mastitis in mice.Mechanistically,ruminal dysbiosis increases gastrointestinal inflammation and compromises the integrity of the gastrointestinal barrier,thereby facilitating the translocation of harmful bacterial components,metabolites,and pathobionts into the bloodstream.This disruption impairs blood-milk barrier function,leading to systemic inflammation and the development of mastitis.In this review,we summarize recent advances in understanding how ruminal dysbiosis induces mastitis and explore potential prevention and control strategies targeting the modulation of ruminal microbiota. 展开更多
关键词 Bacterial translocation Mastitis Metabolic disorder Pathogenesis Ruminal microbiota
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Source-dependent variability in gut barrier disruption,bacterial translocation,and immune activation after ischemic stroke in Wistar rats 认领 引用
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作者 Cristina Granados-Martinez Nuria Alfageme-Lopez +8 位作者 Manuel Navarro-Oviedo Victor Mora-Cuadrado David Sevillano-Fernandez Luis Alou-Cervera Maria Encarnación Fernandez-Valle Olivia Hurtado Maria AMoro Ignacio Lizasoain Jesus Miguel Pradillo 《Animal Models and Experimental Medicine》 CAS CSCD 2026年第2期389-398,共10页
Stroke induces profound neuroinflammation and systemic immune dysregulation,including disturbances in gut homeostasis.Experimental evidence suggests that intestinal barrier permeability(IBP)and bacterial translocation... Stroke induces profound neuroinflammation and systemic immune dysregulation,including disturbances in gut homeostasis.Experimental evidence suggests that intestinal barrier permeability(IBP)and bacterial translocation(BT)critically influence stroke outcomes.However,biological variability among commonly used rodent substrains has received limited attention.In this pilot study,we compared poststroke immune responses in two Wistar rat substrains obtained from different suppliers:RccHan(Envigo)and RjHan(Janvier).Naive animals(n=4)and rats subjected to permanent cerebral ischemia(n=8 per substrain)were evaluated 72 h after middle cerebral artery occlusion and stratified according to the presence or absence of BT.Immune cell populations in blood and bone marrow were analyzed using flow cytometry,and leukocyte infiltration into ischemic brain tissue was quantified using immunohisto-chemistry.Differences were considered statistically significant when p<0.05.Both substrains developed significant infarcts and neurological deficits.RccHan rats exhibited larger infarct volumes and more extensive BT across multiple organs.In contrast,RjHan rats exhibited BT mainly confined to mesenteric lymph nodes but exhibited greater IBP.Although dissemination was broader in RccHan rats,overall bacterial burden was slightly lower compared with RjHan,and extraintestinal bacterial composition differed between groups.Particularly,RjHan rats exhibited stronger systemic and central immune activation,with significant alterations in lymphocyte and monocyte populations and enhanced granulocyte and T-cell infiltration within ischemic lesions.These findings demonstrate that substrain origin profoundly influences poststroke intestinal barrier integrity,bacterial dissemination,and immune responses considering substrain-related variability is essential to improve reproducibility and translational relevance in preclinical stroke research. 展开更多
关键词 bacterial translocation intestinal barrier neuroinflammation stroke vendor effects Wistar rats
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Age-dependent nuclear accumulation of the membrane-associated NAC transcription factor GER1 activates SA biosynthesis to promote leaf senescence in Arabidopsis 认领 引用
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作者 Yi Wang Yutao Wang +5 位作者 Fengying An Yu Cao Ping Chen Xuanzhi Wang Hongwei Guo Genji Qin 《Molecular Plant》 SCIE CAS CSCD 2026年第7期1452-1472,共21页
Leaf senescence is a programmed physiological process that enhances nutrient use efficiency,thereby promoting plant adaptation and crop productivity.However,the molecular mechanisms that govern the initiation and prec... Leaf senescence is a programmed physiological process that enhances nutrient use efficiency,thereby promoting plant adaptation and crop productivity.However,the molecular mechanisms that govern the initiation and precisely control the progression of senescence remain poorly understood.Here,we identify GERAS1(GER1),a membrane-associated NAC transcription factor that acts as an age-dependent integrator of the leaf senescence program.As leaves age,GER1 expression is induced,and its protein undergoes age-dependent translocation from the plasma membrane to the nucleus.The activation tagging mutant ger1-D exhibits early leaf senescence,while the disruption of GER1 and its three homologs in a quadruple ger1 ger2 ger3 ger4 mutant causes significantly delayed leaf senescence.We further show that the nucleus-localized GER1 directly binds and activates the promoter of the central senescence regulator ORE1.Moreover,GER1 and ORE1 form a transcriptional activation complex that directly upregulates SID2,which encodes a key salicylic acid(SA)biosynthetic enzyme.The accumulation of SA in turn promotes the expression of GER1,establishing a positive feedforward loop that drives leaf senescence.Taken together,these findings not only unveil that GER1 acts as a central spatiotemporal regulator of leaf senescence,but also uncover a straightforward membrane-to-nucleus signaling pathway that finely regulates leaf aging. 展开更多
关键词 leaf senescence GER1 transcription factor protein translocation ORE1 SID2
Microbiome-immune interactions in autoimmune liver diseases 认领 引用
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作者 Yana Kiseleva Roman Maslennikov +5 位作者 Elena Poluektova Oksana Zolnikova Alexey Sigidaev Yury Zharikov Elena Shirokova Vladimir Ivashkin 《World Journal of Gastroenterology》 SCIE CAS 2026年第12期7-38,共32页
The impact of gut microbiota on immune regulation has received increasing attention in recent years,particularly its role in immune-mediated liver diseases such as autoimmune hepatitis(AIH),primary biliary cholangitis... The impact of gut microbiota on immune regulation has received increasing attention in recent years,particularly its role in immune-mediated liver diseases such as autoimmune hepatitis(AIH),primary biliary cholangitis(PBC),primary sclerosing cholangitis(PSC),and immunoglobulin G4(IgG4)-related sclerosing cholangitis(IgG4-SC).Disturbances in gut microbiota composition(gut dysbiosis)compromise the intestinal barrier and trigger abnormal immune activity,which foster persistent inflammation,autoimmunity and subsequent liver damage.An increase in Veillonella,Streptococcus,and Lactobacillus were found in most studies for all four diseases.A decrease in Clostridium was detected in AIH and PBC,but an increase in these bacteria is observed in PSC and IgG4-SC.Other common changes in AIH and PBC were an increase in Klebsiella and a decrease in Ruminococcus.In contrast,Ruminococcus is often found to be elevated in PSC.Common features of PBC and PSC are an increase in Enterococcus and a decrease in Faecalibacterium,Enterobacterium,and Coprococcus.Gut microbiome changes in IgG4-SC are most similar to PSC.Probiotics have positive effects in AIH and cholestatic liver diseases in small randomized controlled trial(RCTs).Positive effects of butyrate were in mice models of AIH and PBC.A small study found a positive effect of butyrate on patients with PBC.Indole-3-carboxaldehyde reduced the inflammatory activity of Tcells in patients with AIH in vitro and reduced inflammation in a PSC mouse model.Fecal microbiota transplantation showed beneficial effects in mouse models of AIH and in patients with PSC.Many studies demonstrated the effectiveness of microbiota-targeted therapy on mouse models of autoimmune liver diseases.However,these results remain to be verified in RCTs involving humans. 展开更多
关键词 Microbiota Microbiome Bacterial translocation Dysbiosis Gut-liver axis Gut-immunity axis
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Weed Species Associated with Cacao Cultivation Exhibiting Phytoremediation Potential for Cadmium and Lead:A Study Based on Bioconcentration and Translocation Factors 认领 引用
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作者 Lorena E.Romero-Chávez Emilee Calero-Rios +5 位作者 Uriel Aldava-Pardave Elvis Ottos-Díaz Noelito Salgado-Veramendi Eldhy S.Huerto-Pajuelo Carolina Ibarra-Porras Richard A.Solórzano-Acosta 《Phyton-International Journal of Experimental Botany》 SCIE 2026年第6期346-365,共20页
The exploration and identification of spontaneous weed species in heavy metal–contaminated soils represent a relevant approach for understanding the role and their potential application in phytoremediation.In cacao c... The exploration and identification of spontaneous weed species in heavy metal–contaminated soils represent a relevant approach for understanding the role and their potential application in phytoremediation.In cacao cultivation,cadmium contamination poses a significant risk due to the restrictions established for soils and cacao-derived products,thereby threatening productive sustainability and export viability.The objective of this study was to identify weed species associated with cacao cultivation exhibiting accumulation patterns and phytoremediation potential for Cd and Pb,through the assessment of biomass production and the bioconcentration factor(BCF)and translocation factor(TF),in natural conditions.Soil samples were collected from seven cacao-growing zones,and the site with the highest Cd concentration(1.65 mg kg−1)was selected,where ten dominant weed species were identified and evaluated.Significant interspecific differences were observed in biomass production and in Cd and Pb concentrations(p<0.05).Talinum paniculatum exhibited the highest total biomass(6.15 g)and the highest TF for Cd,whereas Cyperus aggregatus showed the greatest total Cd accumulation(2.68 mg kg−1).BCF and TF values enabled the identification of species with accumulation patterns consistent with Cd phytoextraction(T.paniculatum,C.aggregatus,Pseudelephantopus spiralis,Bidens riparia,Euphorbia heterophylla,and Malvastrum coromandelianum)and with phytostabilization(Ocimum campechianum).For Pb the observed patterns were consistent with potential phytoextractive behavior in B.riparia,P.spiralis,E.heterophylla,Hilleria latifolia,and Acalypha stachyura.Although these results do not constitute functional evidence of remediation,they describe heavy metal accumulation and translocation patterns in native weed species,providing a technical basis for the design of future experimental trials aimed at the sustainable remediation of contaminated soils. 展开更多
关键词 Spontaneousweeds cadmium lead plant biomass bioconcentration factor translocation factor rhizosphere
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Thinned young apple polyphenol prevents diabetic cardiomyopathy through promoting TET2-mediated active DNA demethylation in STZ-induced diabetic mice heart 认领 引用
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作者 Run Liu Zhongshi Qi +7 位作者 Liying Zhou Jiacheng Fang Shengjie Pei Yanlei Yuan Chunyan Tian Yiding Zhang Lei Chen Duo Li 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第3期1120-1131,共12页
Diabetic cardiomyopathy(DCM)refers diabetic patients develop cardiomyopathy characterized by cardiac systolic and diastolic dysfunction.Currently,no research has explored the role of ten-eleven translocation(TET)-medi... Diabetic cardiomyopathy(DCM)refers diabetic patients develop cardiomyopathy characterized by cardiac systolic and diastolic dysfunction.Currently,no research has explored the role of ten-eleven translocation(TET)-mediated active DNA demethylation in the development of DCM.Through case-control study,we found that the level of 5-hydroxymethylcystein in peripheral blood DNA of DCM patients was significantly lower than it in the healthy subjects and diabetic patients.Secondly,we had conducted an animal study to explore the effect of thinned young apple polyphenol(TYAP)on DNA demethylation in streptococci-induced diabetic mice.TYAP effectively prevented the ventricular dysfunction and cardiomycyte disarray via alleviating DNA epigenetic modifications metabolic disorders in diabetic mice heart.TYAP increased the stability of TET2 protein via activating phosphorylate AMPK and enhanced the activity of TETs enzymes via improving tricarboxylic acid cycle,then promoted TET-mediated active DNA demethylation.TYAP prevented the occurrence of DCM by promoting TET2-mediated active DNA demethylation in the heart of diabetic mice. 展开更多
关键词 Diabetic cardiomyopathy Thinned young apple polyphenol Active DNA demethylation Ten-eleven translocation 2 Tricarboxylic acid cycle AMPK
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The dose-dependent early responses of tomato plants under low Ni exposure:a focus on roots and leaves 认领 引用
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作者 Greta Ricci Alessandra Francini +1 位作者 Andrea Raffaelli Luca Sebastiani 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第7期1719-1723,共5页
In plants,nickel(Ni)occurs naturally at 0.01–5μg·g−1dry weight and plays essential roles in physiological processes from germination to fruit development(Seregin and Kozhevnikova,2006).However,excessive Ni d... In plants,nickel(Ni)occurs naturally at 0.01–5μg·g−1dry weight and plays essential roles in physiological processes from germination to fruit development(Seregin and Kozhevnikova,2006).However,excessive Ni disrupts growth,metabolism,enzyme activity,stomatal function,and chlorophyll integrity,ultimately impairing water relations(Heidari et al.,2020;Valivand and Amooaghaie,2021a,2021b).Ni also interferes with mineral uptake and translocation in crops,such as zucchini,tomato,and lettuce(Valivand and Amooaghaie,2021a,2021b;Fortini et al.,2025).Owing to its toxicity and health risks(Das et al.,2019),strict regulatory limits have been established to limit Ni exposure.In Italy,the Ministerial Decree on wastewater reuse sets a maximum Ni concentration of 0.2 mg·L−1for long-term irrigation. 展开更多
关键词 nickel exposure mineral uptake translocation water relations heidari root responses leaf responses tomato plants physiological processes dose dependent
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The T1DL.1VS translocation harboring the Glu-V1/Pm67 loci could accelerate the improvement of disease resistance and quality in wheat 认领 引用
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作者 Runze Zhang Jin Hu +4 位作者 Lingna Kong Liping Xing Aizhong Cao Yingxin Zhong Ruiqi Zhang 《The Crop Journal》 SCIE CSCD 2026年第3期1072-1078,共7页
A key objective of wheat(Triticum aestivum)breeding is the simultaneous improvement of disease resistance and bread making quality.The Glu-V1 locus encoding the high-molecular-weight glutenin subunit(HMW-GS)V71 and th... A key objective of wheat(Triticum aestivum)breeding is the simultaneous improvement of disease resistance and bread making quality.The Glu-V1 locus encoding the high-molecular-weight glutenin subunit(HMW-GS)V71 and the powdery mildew resistance gene Pm67 were previously identified on chromosome arm 1 VS of the wild wheat species Dasypyrum villosum.Here,we generated the T1 DL.1V#4S translocation line NAU195,which carried the V71 subunit for improved bread-making quality but was susceptible to all 18 isolates of the powdery mildew fungus Bgt examined.By contrast,the previously developed T1 DL.1V#5S line NAU196 harbors the Pm67 gene conferring broad-spectrum powdery mildew resistance but lacks the V71 subunit.Genetic analysis of F1 and F2 progenies from a cross between NAU195 and NAU196 confirmed that Pm67 is a single dominant gene.By screening 2954 F2 plants,we fine mapped Pm67 to an approximately 2.1 Mb interval on chromosome arm 1 VS in the reference genome.We identified 35 high-confidence protein-coding genes in this region,including eight candidate genes encoding nucleotide binding site leucine rich repeat(NLR)proteins.We identified 12 recombinant T1 DL.1 VS translocation lines harboring both the V71 subunit and Pm67 genes,and introgressed one of the corresponding translocations into the high-yielding cultivar NMZ119.Plant development in the resulting T1 DL.1 VS translocation lines harboring the V71 subunit and Pm67 genes was comparable to that of NMZ119,with no significant yield penalty but with markedly improved powdery mildew resistance and gluten strength.Overall,our strategy for mapping and pyramiding alien genes in the common wheat background allowed us to generate a valuable genetic resource and molecular tool for accelerating the concurrent improvement of powdery mildew resistance and bread making quality. 展开更多
关键词 T1DL·1VS translocation Pm67 gene Glu-V1locus HMW-GS V71 subunit
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Nanopore-based Manipulation and Separation of Single-stranded DNA Molecules through Tuning pH Values 认领 引用
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作者 Cheng-Kai Zhou Sheng-Wen Miao Li-Zhen Sun 《Chinese Journal of Polymer Science》 SCIE EI CAS CSCD 2026年第7期2361-2369,共9页
The unique regulatory effect of pH on electrostatic interactions offers a powerful approach for manipulating and separating singlestranded DNA(ssDNA)molecules.In this study,we employ Langevin dynamics simulations to i... The unique regulatory effect of pH on electrostatic interactions offers a powerful approach for manipulating and separating singlestranded DNA(ssDNA)molecules.In this study,we employ Langevin dynamics simulations to investigate the translocation dynamics of two ssDNAs,poly(dA)and poly(dT),through a silicon nitride nanopore under acidic conditions.The key distinction between the two chains is their different pH-dependent protonation.At low pH,the highly protonated adenine bases experience repulsive interactions from the similarly protonated nanopore surface and the retarding force from the external voltage,whereas neutral thymine bases do not.Consequently,compared to poly(dT),poly(dA)exhibits a lower capture probability and slower translocation speed under strongly acidic conditions.However,the difference in the translocation behaviors between the two chains gradually diminishes as the pH increases.Based on their distinct pH-dependent behaviors,poly(dA)and poly(dT)of identical length can be successfully separated through the translocation strategy at low pH,even when they are initially mixed on the same side of the nanopore. 展开更多
关键词 Single-stranded DNA Nanopore translocation Electrostatic interaction pH value Langevin dynamics simulation
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An erucin-anchored polymer with excellent amyloidosis inhibition and direct cell membrane penetration ability for intracellular protein delivery 认领 引用
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作者 Le Chen Hui Wang Yiyun Cheng 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第4期721-726,共6页
Alzheimer's disease(AD)is a complex and multifactorial neurodegenerative disorder,marked by a variety of pathological hallmarks such as oxidative stress(OS),metal accumulation,and the aggregation of amyloid-beta(A... Alzheimer's disease(AD)is a complex and multifactorial neurodegenerative disorder,marked by a variety of pathological hallmarks such as oxidative stress(OS),metal accumulation,and the aggregation of amyloid-beta(Aβ)proteins.Erucin,a natural compound present in cruciferous plants,has demonstrated promising therapeutic potential in modulating neurodegenerative diseases,hinting at its neuroprotective capabilities.In this study,we engineered an innovative intracellular protein delivery system centered around erucin.This delivery platform operates through a cell membrane perforation mechanism,enabling the swift translocation of target proteins into the cytoplasm.As a result,it substantially shortens the time required for the proteins to exert their functions.Significantly,this delivery system inherently possesses the capacity to inhibit Aβaggregation.In PC12 cell models,the delivery of the antioxidant enzyme superoxide dismutase(SOD)successfully mitigated the OS triggered by Aβaggregation and decreased cytotoxicity.This multifaceted therapeutic strategy holds great promise as an effective approach for the treatment of AD. 展开更多
关键词 Alzheimer’s disease Aβaggregation Protein delivery Rapid translocation Oxidative stress
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WTAP-mediated N6-methyladenosine modification of circ_PVT1 promotes proliferation and tenogenic differentiation of tendon stem/progenitor cells 认领 引用
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作者 Yu Chen Xu Hu +3 位作者 Ren-Zhi Gao Shi-Guo Zhou Xiao-Tian Jia Quan-Shan Hou 《World Journal of Stem Cells》 SCIE 2026年第4期103-114,共12页
BACKGROUND Tendon stem/progenitor cells(TSPCs)are a novel type of stem cell.TSPCs share common characteristics with stem cells,including their proliferation,pluripotency,and self-renewal abilities.Circular RNA plasmac... BACKGROUND Tendon stem/progenitor cells(TSPCs)are a novel type of stem cell.TSPCs share common characteristics with stem cells,including their proliferation,pluripotency,and self-renewal abilities.Circular RNA plasmacytoma variant translocation 1(circ_PVT1)has been reported to inhibit senescence in TSPCs.However,the mechanism by which circ_PVT1 regulates the proliferation and differentiation of TSPCs remains unclear.AIM To explore how circ_PVT1 regulates the proliferation and differentiation of TSPCs.METHODS Mouse TSPCs were isolated from the Achilles tendon of 12 male C57Bl/6 mice at postnatal day 30,and 5 ng/mL of transforming growth factor(TGF)-β1 was used to induce the tenogenic differentiation in TSPCs.Picro-Sirius red staining was used to assess the proliferation,migration,and apoptosis of TSPCs.Biochemical kits were used to determine the levels of reactive oxygen species,malondialdehyde,glutathione,superoxide dismutase,and ATP.Then,N6-methyladenosine(m6A)dot blot and methylated RNA immunoprecipitation polymerase chain reaction(RIP-PCR)were used to examine the m6A levels.Moreover,RNA pulldown and RIP-PCR were performed to analyze the interaction between WTAP and circ_PVT1.RESULTS TGF-β1 treatment induced tenogenic differentiation of TSPCs.Circ_PVT1 knockdown reversed the increase of tendon-specific protein,cell proliferation,and migration that was induced by TGF-β1 treatment.In addition,circ_PVT1 inhibition promoted oxidative stress and mitochondrial damage in the TGF-β1-induced TSPCs.The m6A modification level of circ_PVT1 was upregulated in the TGF-β1-induced TSPCs.Furthermore,RNA pulldown and RIP-PCR showed that circ_PVT1 interacted with WTAP in TSPCs,and the WTAP-mediated m6A modification of circ_PVT1 regulated the differentiation of TSPCs.CONCLUSION WTAP-mediated m6A modification of circ_PVT1 promotes the proliferation and tenogenic differentiation of TSPCs,thereby indicating a promising therapeutic strategy for tendon repair. 展开更多
关键词 WTAP Circular RNA plasmacytoma variant translocation 1 Tenogenic differentiation Tendon stem/progenitor cells N6-methyladenosine
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Exploring the mechanism and potential treatment of severe acute pancreatitis-associated lung injury based on the gut-lung axis 认领 引用
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作者 Xin-Tong Ye Chu-Ying Yu +3 位作者 Hao-Yu Zhao Su-Ting Qian Ye Huang Qing-Sheng Liu 《World Journal of Gastrointestinal Surgery》 SCIE 2026年第4期24-34,共11页
Severe acute pancreatitis(SAP),a prevalent and life-threatening abdominal emergency,is characterized by its abrupt onset,rapid progression,and high mortality rate,frequently precipitating multiple organ dysfunction sy... Severe acute pancreatitis(SAP),a prevalent and life-threatening abdominal emergency,is characterized by its abrupt onset,rapid progression,and high mortality rate,frequently precipitating multiple organ dysfunction syndromes.Acute lung injury(ALI)represents the most common early complication of SAP.ALI may progress to acute respiratory distress syndrome,which constitutes the primary cause of mortality in patients with SAP.However,the precise pathogenesis underlying ALI in SAP remains incompletely elucidated.Notably,recent advances in gut-lung axis research have significantly advanced our understanding of SAPassociated ALI(SAP-ALI).The gut-lung axis,a sophisticated bidirectional communication network linking the intestines and lungs,facilitates crosstalk between the microbiota and their metabolites across both organs via lymphatic and circulatory systems alongside the mucosal immune system.Critically,research confirms that SAP induces severe gut microbiota dysbiosis.This dysbiosis disrupts the intestinal mucosal barrier,enabling bacterial and endotoxin translocation to the lungs through lymphatic and hematogenous routes.Concurrently,gutderived immune cells migrate to the lungs via the bloodstream,directly contributing to pulmonary immune-inflammatory imbalance.Consequently,this review synthesized the central role of the gut-lung axis in SAP-ALI pathogenesis and explored emerging therapeutic strategies targeting this pivotal axis,aiming to provide novel insights for the clinical prevention and treatment of SAP-ALI. 展开更多
关键词 Severe acute pancreatitis-associated acute lung injury Gut-lung axis Intestinal microbiota Bacterial translocation Migration of immune cells
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Nuclear translocation of pyruvate kinase M2 drives high glucose-induced angiogenesis in retinal endothelial cells via the HIF-1α axis 认领 引用
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作者 Tian-Yi Liu Ya-Jing Tian +6 位作者 Peng-Zhou Kuai Yi-Sheng Luo Cheng-Wei Duan Tian-Peng Chen Xiao-Le Wang Dong-Mei Zhang Xin Cao 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2026年第5期858-868,共11页
AIM:To investigate the role of pyruvate kinase M2(PKM2)in high glucose(HG)-stimulated retinal endothelial cells and its underlying molecular mechanisms and signaling pathways in retinal angiogenesis.METHODS:Human reti... AIM:To investigate the role of pyruvate kinase M2(PKM2)in high glucose(HG)-stimulated retinal endothelial cells and its underlying molecular mechanisms and signaling pathways in retinal angiogenesis.METHODS:Human retinal microvascular endothelial cells(HRMECs)were cultured and divided into the following groups:normal glucose(NG,5.5 mmol/L),HG(30 mmol/L),HG with PKM2 knockdown(HG+shPKM2),and HG treated with the pharmacological activator TEPP‑46(HG+TEPP‑46).Cellular viability,proliferation,migration,and tube‑forming ability were assessed using CCK‑8,EdU,wound healing/Transwell,and Matrigel assays,respectively.The expression levels of PKM2,phosphorylated PKM2(p‑PKM2,Y105),hypoxia-inducible factor-1α(HIF‑1α),and vascular endothelial growth factor A(VEGFA)were detected by Western blotting.The oligomerization status of PKM2 was analyzed via native gel electrophoresis.The subcellular localization of PKM2 was examined by immunofluorescence and nuclear‑cytoplasmic fractionation.RESULTS:Under HG stimulation,the expression level of PKM2 was significantly increased(P<0.05).Knockdown of PKM2 was found to markedly suppress cell viability,proliferation,migration,and tube formation in HRMECs(P<0.05).Mechanistic studies revealed that phosphorylation of PKM2 at the Y105 site was promoted by HG treatment,which induced its dissociation from a tetramer to a dimer,thereby driving its nuclear translocation.Upon entering the nucleus,PKM2 was shown to exert critical non‑metabolic functions;it was physically bound to HIF‑1αand acted as its co‑activator,leading to significant upregulation of VEGFA expression(P<0.05).In contrast,the PKM2 activator TEPP‑46 effectively prevented dimerization and nuclear translocation of PKM2 by promoting its tetramerization.Consequently,the PKM2/HIF‑1αaxis‑mediated upregulation of VEGFA was blocked,ultimately resulting in the reversal of HG‑induced angiogenesis.CONCLUSION:HG influences retinal endothelial cell function by inducing PKM2 phosphor ylation,dimerization,and nuclear translocation.The shift in PKM2 phosphorylation and oligomerization status represents a key mechanism through which TEPP-46 reverses HGinduced angiogenesis. 展开更多
关键词 retinal endothelial cells glucose angiogenesis nuclear translocation pyruvate kinase M2 hypoxia-inducible factor-1α
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