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Dietary supplementation with sodium isobutyrate enhances growth performance and colonic barrier function in weaned piglets via microbiota-metabolite-host interactions 认领 引用 被引量：1
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作者 Xiuyu Fang Zihan Chi +6 位作者 Zhengyi Wang Xinlin Wang Xingrui Qu Shuang Zhang Feng Gao Baoming Shi Xuan Zhao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期404-425,共22页
Background Weaning-induced diarrhoea and growth retardation in piglets are associated with impaired intestinal barrier function and decreased levels of colonic short-chain fatty acids(SCFAs).Although SCFA supplementat... Background Weaning-induced diarrhoea and growth retardation in piglets are associated with impaired intestinal barrier function and decreased levels of colonic short-chain fatty acids(SCFAs).Although SCFA supplementation has been proposed to mitigate these issues,the efficacy and optimal dosage of sodium isobutyrate remain unclear.Results We investigated the effects of sodium isobutyrate supplementation(500,1,000,2,000,and 4,000 mg/kg diet)on weaned piglets(Duroc×Landrace×Yorkshire,28 d of age;n=8).After a 28-d feeding trial,supplementation at 500–2,000 mg/kg significantly improved average daily gain and feed efficiency and reduced diarrhoea frequency,with maximal benefits observed at 1,000 mg/kg(P<0.0001).Additionally,500–1,000 mg/kg sodium isobutyrate supplementation increased the apparent digestibility of crude protein,organic matter,and crude fibre(P<0.05).Serum biochemical parameters were unaffected,although secretory immunoglobulin A(SIgA)levels significantly increased upon supplementation with 500–1,000 mg/kg(P<0.05).16S rRNA gene sequencing indicated that sodium isobutyrate increased the abundance of beneficial colonic microbiota.The 1,000 mg/kg group presented the most pronounced effect,with a significant increase of the relative abundance of Prevotella and the greatest improvement in SCFA concentrations(P<0.05).Metabolomics revealed elevated levels of colonic indole-3-lactic acid and 3-hydroxybutyrate upon supplementation with 1,000 mg/kg(P<0.05).Transcriptomic analyses indicated activation of protein digestion and absorption pathways,and PI3K-Akt signalling,marked by TSG-6 upregulation and the suppression of ISG15 and DDIT4 expression(P<0.05).Supplementation with 1,000 mg/kg was associated with improved intestinal barrier-related markers,including reduced serum D-lactate,diamine oxidase,and lipopolysaccharide levels,increased tight junction protein expression;activation of G protein-coupled receptors;and inhibition of TLR4/MyD88/NF-κB signalling(P<0.05),suggesting enhanced barrier function.Conclusions In conclusion,dietary supplementation with 1,000 mg/kg sodium isobutyrate was associated with improved intestinal morphology,reduced serum permeability,increased expression of tight junction proteins,and enhanced immune function in weaned piglets,suggesting enhanced colonic barrier function and providing dosage guidance and mechanistic insights for future applications. 展开更多
关键词 Colonic barrier function Diarrhoea Intestinal microbiota Sodium isobutyrate Weaned piglets
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Multi-omic analysis for dietary supplementation of different ratios of soluble and insoluble fiber on intestinal microbiota,metabolites and inflammation of weaned piglets 认领 引用
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作者 Yetong Xu Chengyu Zhou +7 位作者 Yingying Lu Xutong Guo Minyue Zong Junwei Zhu Pan Zhou Jiaman Pang Xie Peng Zhihong Sun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第4期1606-1618,共13页
Different types of dietary fiber(DF)play important roles in enhancing intestinal health and overall performance in animals.This study investigated the effects of high-DF diets containing different ratios of soluble to... Different types of dietary fiber(DF)play important roles in enhancing intestinal health and overall performance in animals.This study investigated the effects of high-DF diets containing different ratios of soluble to insoluble dietary fiber(SDF:IDF)on growth performance,intestinal barrier integrity,microbiota,and metabolite profiles in weaned piglets.The four dietary treatments consisted of a basal diet(CON)and three high-DF diets with SDF:IDF ratios of 0.37,0.25,and 0.13(designated HF-0.37,HF-0.25,and HF-0.13,respectively).On days 14 and 28,colonic tumor necrosis factor-α,interleukin-1β,interleukin-6,and interleukin-8 concentrations were higher in the HF-0.37 group than in the CON,HF-0.25,and HF-0.13 groups(P<0.05).Plasma D-lactate and endotoxin levels were lower in the HF-0.25 group compared to the CON group at both time points(P<0.05).Furthermore,colonic zonula occludens 1 expression was upregulated in the HF-0.25 and HF-0.13 groups compared to the CON and HF-0.37 groups on day 14(P<0.05).At the transcriptional level,all three high-DF diets modulated signaling pathways associated with inflammation and immune responses in the colon.Notably,DF supplementation particularly the HF-0.25 diet upregulated colonic levels of 3-indole butyric acid,nicotinic acid,and 3-methylthiopropylamine on d 14 and reduced certain peptide levels by d 28.These findings indicate that DF supplementation,especially at an SDF:IDF ratio of 0.25,exerts beneficial effects on intestinal integrity in weaned piglets,potentially mediated by alterations in colonic metabolite profiles,whereas HF-0.37 and HF-0.13 exhibited limited impacts on intestinal barrier function. 展开更多
关键词 weaned piglets soluble fiber insoluble fiber multi-omics intestinal integrity
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Integrative clustering by colonic volatile fatty acids reveals microbiota and host physiological variation in weaned piglets 认领 引用
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作者 Chengcheng Li Stijn Heirbaut +5 位作者 Joris Michiels Yuhuang Hou Anneke Ovyn Noémie Van Noten Elout Van Liefferinge Jeroen Degroote 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第3期1554-1572,共19页
Background Volatile fatty acids(VFAs)reflect microbial fermentation linking gut microbiota,metabolism and host physiology.However,endogenous VFA profiles and their physiological relevance remain insufficiently charact... Background Volatile fatty acids(VFAs)reflect microbial fermentation linking gut microbiota,metabolism and host physiology.However,endogenous VFA profiles and their physiological relevance remain insufficiently characterized.This study aimed to identify distinct VFA phenotypes in weaned piglets and reveal how microbial fermentation patterns connect gut metabolism with animal health and performance.Results In this study,we integrated six independent trials comprising 164 weaned piglets.Here,k-means clustering of six VFAs in colonic digesta to identified two metabolite phenotypes:high level of short-chain fatty acids(HSCFA)and high level of branched-chain fatty acids(HBCFA).Generally,The HSCFA group exhibited significantly higher concentrations of VFA(P<0.001),and lower levels of ammonia and serum urea(P<0.001),consistent with enhanced carbohydrate fermentation and reduced nitrogen output.Additionally,the HSCFA group had significantly greater villus height in the distal small intestine(P<0.01),lower mid-colonic pH(P<0.001)and thrombocyte counts(P<0.01).In contrast,the HBCFA group showed elevated levels of branched-chain fatty acids and ammonia(P<0.001),as well as enrichment of Escherichia-Shigella,Rikenellaceae dgA-11 gut group and Prevotella(adjusted P<0.05).The HSCFA group was enriched in Lactobacillus,Mitsuokella and Dialister(adjusted P<0.05),and exhibited higher final body weight and average daily gain(P<0.05),indicating better growth performance.To explore associations between gut microbiota and VFA phenotypes,an XGBoost classification model was trained,based on genus-level composition,to predict the metabolic phenotype.It achieved a moderate accuracy and identified Unclassified Eubacterium coprostanoligenes group,Lactobacillus and Escherichia-Shigella as important genera contributing to group separation.Conclusions Clustering based on colonic VFA profiles identified distinct microbial and physiological patterns that may influence gut function and growth performance in weaned piglets.The enrichment of beneficial microbes and fermentation products in the HSCFA group suggests a more favorable intestinal environment,while protein fermentation in the colon seems associated with reduced animal performance and less beneficial intestinal environment. 展开更多
关键词 Branched-chain fatty acids Microbiota Short-chain fatty acids Weaned piglets
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Fecal microbiota transplantation mitigates lipopolysaccharide-induced oxidative stress in weaned piglets by modulating gut microbiota and enhancing riboflavin metabolism 认领 引用 被引量：1
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作者 Jixiang Ma Mengqi Liu +3 位作者 Junying Xu Boshuai Liu Yalei Cui Yinghua Shi 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期1002-1019,共18页
Background During the weaning phase,piglets are exposed to significant physiological and environmental stressors,which disrupt the balance of their intestinal microbiota and often lead to severe diarrhea.Previous stud... Background During the weaning phase,piglets are exposed to significant physiological and environmental stressors,which disrupt the balance of their intestinal microbiota and often lead to severe diarrhea.Previous studies have demonstrated that alfalfa fiber,derived from the stems and leaves of alfalfa,can effectively alleviate diarrhea in piglets.Additionally,multiple studies have highlighted the potential of fecal microbiota transplantation(FMT)in mitigating diarrhea in various models of intestinal diseases in young animals.However,the specific mechanisms by which FMT from targeted sources alleviates diarrhea in weaned piglets remain to be fully elucidated.Results In this study,FMT from donor piglets fed an alfalfa fiber-supplemented diet effectively alleviated diarrhea,improved intestinal morphology,and enhanced gut barrier function in weaned piglets.FMT further promoted the colonization of beneficial bacterial genera(including UCG-005,unclassified Lachnospiraceae,Lachnospiraceae AC2044 group,UCG-002,Candidatus Saccharimonas,and Lachnospiraceae ND3007 group)while inhibiting the detrimental genus Tyzzerella,consequently enhancing the production of short-chain fatty acids(SCFAs).Additionally,FMT upregulated riboflavin metabolism,leading to elevated flavin adenine dinucleotide(FAD)levels and increased glutathione reductase activity,thereby collectively attenuating lipopolysaccharide(LPS)-induced oxidative stress and contributing to intestinal health.Conclusions We found that FMT modulates the structure of the gut microbiota,enhances microbial diversity and composition,increases the production of SCFAs,and upregulates riboflavin metabolism to elevate FAD levels.These changes collectively enhance immune and antioxidant capacities,thereby alleviating diarrhea. 展开更多
关键词 Fecal microbiota transplantation Flavin adenine dinucleotide Lipopolysaccharide Oxidative stress Short-chain fatty acids Weaned piglets
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Dietary supplementation with Bacillus-based probiotic improves gut health in the weaned piglets challenged by rotavirus 认领 引用
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作者 Fengyu Xiang Heng Yang +15 位作者 Xiangqi Fan Dayan Tan Bing Huang Bing Yu Jun He Yuheng Luo Junqiu Luo Hui Yan Junning Pu Jianping Wang Quyuan Wang Huifen Wang John Kyaw Htoo Santa Maria Mendoza Guiling Yan Xiangbing Mao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期951-961,共11页
Background As probiotics,Bacillus strains may regulate some physiological functions in animals.This study aimed to evaluate whether dietary supplementation with a Bacillus-based probiotic could alleviate gut damage in... Background As probiotics,Bacillus strains may regulate some physiological functions in animals.This study aimed to evaluate whether dietary supplementation with a Bacillus-based probiotic could alleviate gut damage induced by rotavirus(RV)infection in piglets.Twenty-four piglets were randomly assigned into 2 groups fed with the basal diet(n=16)and the diet containing 109 colony-forming unit Bacillus spores/kg(n=8).On d 8,8 piglets fed with the diet supplemented with Bacillus-based probiotic and 8 piglets fed with basal diet were orally infused with RV,while the residue piglets had oral gavage of sterile essential medium.The trial duration was 12 d.Results RV challenge induced diarrhea,significantly destroyed the morphology of jejunal mucosa(P<0.05),significantly increased RV-antibody and RV non-structural protein 4 of jejunal mucosa(P<0.05),significantly impaired antioxidant capacity(including malondialdehyde level,total antioxidant capacity and catalase activity),immunity(such as interleukin 2,interleukin 4 and secreted immunoglobulin A levels),mucins and the m RNA expression of tightjunction-related(such as Zonula occludens 1,occludin)and apoptotic-related(including B-cell lymphoma/leukaemia-2-associated X protein,B cell lymphoma/leukaemia-2,cysteinyl aspartate specific proteinases)genes of jejunal mucosa(P<0.05),and,to some extents,affected the bacteria community structure and abundance of ileal digesta in piglets.However,Bacillus-based probiotic administration could significantly attenuate the negative effects of RV infection on gut health of piglets(P<0.05).Conclusions These findings suggested that supplementing Bacillus-based probiotic in the diet could decrease diarrhea rate,and improve gut health in weaned piglets,which was associated with regulating intestinal antioxidant capacity,apoptosis,and microbiota. 展开更多
关键词 Apoptosis Antioxidant capacity Bacillus strains Intestinal health Microbiota Rotavirus Weaned piglets
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Butyrate combined with niacin enhances intestinal barrier function repair in weaned piglets infected with ETEC by promoting colonic metabolism and antimicrobial peptide expression 认领 引用
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作者 Qingsong Tang Rui Zhen +9 位作者 Bijing Yang Zhenyan Miao Yangyang Wei Shihui Ruan Yiyi He Yunxia Xiong Qiwen Wu Li Wang Zongyong Jiang Hongbo Yi 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第4期2273-2292,共20页
Background Weaning piglets are highly susceptible to enterotoxigenic Escherichia coli(ETEC)infections,which can cause intestinal barrier function dysfunction and death.However,there is still a lack of efficient,econom... Background Weaning piglets are highly susceptible to enterotoxigenic Escherichia coli(ETEC)infections,which can cause intestinal barrier function dysfunction and death.However,there is still a lack of efficient,economical,and safe nutritional interventions.This study aimed to investigate the effects of combining butyrate with niacin on intestinal barrier function repair and resistance to ETEC infection in weaned piglets.In this study,two 14-d animal experiments were designed to observe the optimal butyrate-to-niacin ratio and assess their responses to the ETEC challenge.Results Supplementation with butyrate and niacin at a ratio of 100:2(2,000 mg/kg butyrate and 40 mg/kg niacin,BN2)increased the average daily gain(ADG)and reduced the diarrhea incidence.We also observed an increase in the levels of nicotinamide adenine dinucleotide(NAD)in the colon of weaned piglets.Notably,BN2 promoted amino acid anabolism in the colon and enhanced glycolysis and the tricarboxylic acid(TCA)cycle by increasing the acetylation of key enzymes in the TCA.Furthermore,BN2 enhanced the expression of indispensable genes for the colonic mucosal barrier,including antimicrobial peptides such as porcineβdefensin 1(pBD1),porcineβdefensin 2(pBD2),and proline-arginine rich 39-amino acid peptide(PR39),tight junction proteins,and improved colonic microbiome composition.Based on these findings,we found that BN2 alleviated growth restriction and diarrhea,and modulated the expression of antimicrobial peptides,tight junction proteins,and cytokines to reduce colonic barrier function dysfunction in weaned piglets challenged with ETEC.Mechanistically,we confirmed that BN2elevated the protein expression of acetylation of histone 3 lysin 27(H3K27ac)and enhanced the binding of acH3K27to the promoter regions of pBD1 and PR39.Conclusions Supplementation with BN2 improved growth performance,supported colonic barrier function repair,and enhanced disease resistance in weaned piglets challenged with ETEC.This offers new insights into nutritional strategies for intestinal barrier function repair of piglets infected with ETEC. 展开更多
关键词 Butyrate Colonic barrier function Enterotoxigenic Escherichia coli Niacin Weaned piglets
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Gut microbiota-driven IL-17/PPAR axis mediates epigallocatechin-induced intestinal repair in weaned lambs 认领 引用
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作者 Yuwei Zhao Zhuo Wang +4 位作者 Dingkun Fan Jixian Zhang Yan Tu Qiyu Diao Kai Cui 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第3期1729-1751,共23页
Background Early weaning is a key strategy to improve lamb production efficiency;however,it inevitably compromises intestinal barrier integrity and function.This study aimed to investigate the effects of epigallocatec... Background Early weaning is a key strategy to improve lamb production efficiency;however,it inevitably compromises intestinal barrier integrity and function.This study aimed to investigate the effects of epigallocatechin(EGC)on growth performance and intestinal barrier function in weaned lambs,using metagenomics,metabolomics,and intestinal transcriptomics to elucidate the underlying mechanisms.Results Weaning induced oxidative stress,inflammation,and metabolic disruptions in the jejunum.Supplementation with 12.5 mg/kg EGC(LE)significantly improved growth performance,reduced diarrhea incidence(P<0.05),enhanced mucosal antioxidant capacity(P<0.001),and strengthened anti-inflammatory ability(P<0.001).Metagenomic analysis showed that the LE intervention enriched Ruminococcus spp.and reduced the abundance of Slackia.This microbial shift was associated with elevated luminal concentrations of valeric acid and microbial metabolites derived from EGC.Transcriptomic profiling revealed that the intervention upregulated the PPAR signaling pathway,which supports nutrient metabolism and barrier repair.Concurrently,it attenuated aberrant IL-17 signaling and promoted the restoration of mucosal immune homeostasis,indicating a resolution of excessive inflammatory responses.Conclusions Supplementation with 12.5 mg/kg EGC alleviates weaning stress by fostering a beneficial gut microbiota and promoting the production of specific metabolites.These changes reactivate PPAR mediated epithelial repair and dampen pathological immune activation.Low-dose EGC is an effective nutritional strategy to improve intestinal health and growth in weaned ruminants. 展开更多
关键词 Epigallocatechin Gut Microbiota Inflammation Lamb Weaning
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Piceatannol enhances antioxidant capacity and growth in weaned piglets by regulating of Nrf2-mediated redox homeostasis and modulating of the related gut microbiota 认领 引用
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作者 Longlong Zhu Yuyan Che +4 位作者 Meixia Chen Long Cai Qiujue Wu Tao Feng Jing Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期935-950,共16页
Background Piglets are highly susceptible to oxidative stress,which can reduce growth performance and cause intestinal damage.Piceatannol(PIC),a natural bioactive substance enriched in Chinese rhubarb(Rheum officinale... Background Piglets are highly susceptible to oxidative stress,which can reduce growth performance and cause intestinal damage.Piceatannol(PIC),a natural bioactive substance enriched in Chinese rhubarb(Rheum officinale)and certain dark purple fruits,shows excellent antioxidant properties in our previous cell-based high-throughput screening.However,its effect on piglet growth performance and antioxidant capacity as well as underling mechanism has not been thoroughly investigated.Methods One hundred weaned pigs(28 days of age,8.71±0.20 kg)were randomly assigned to 4 treatments with 5 replicates of 5 pigs per replicate.The experimental diets consisted of:1)basal diet,2)basal diet+100 mg/kg PIC,3)basal diet+200 mg/kg PIC,and 4)basal diet+300 mg/kg PIC.On d 15 and 35,one pig from each replicate was selected for sampling.The growth performance was monitored during a 35-day trial.In addition,H2O2-challenged IPEC-J2 cells served as an in vitro model to investigate the antioxidant mechanisms of PIC.IPEC-J2 cells were treated with 1,000μmol/L H2O2in the presence or absence of 10μmol/L PIC.Results Dietary PIC at 200 mg/kg significantly enhanced growth performance,as evidenced by increased average daily gain and feed conversion rate(P<0.05).PIC supplementation markedly improved systemic antioxidant capacity,with elevated serum total antioxidant capacity,catalase activity,and glutathione levels,along with reduced malondialdehyde content(P<0.05).Notably,PIC modulated the gut microbiota composition,increasing the amounts of beneficial genera(e.g.,Blautia and Faecalibacterium),and these microbial shifts significantly correlated with improved antioxidant indices.In vitro,PIC pretreatment effectively protected IPEC-J2 cells against H2O2-induced oxidative damage by reducing reactive oxygen species generation and lipid peroxidation(P<0.01).Mechanistically,PIC exerts its antioxidant effects through Nrf2 pathway activation,upregulating endogenous antioxidant enzymes(P<0.05)while simultaneously inhibiting apoptosis via the regulation of the Bcl-2/Bax ratio and caspase-3 cleavage(P<0.01).Conclusions PIC improved the growth performance and health status of weaned piglets through the regulation of Nrf2-mediated redox homeostasis and modulation of the related gut microbiota,offering a potential new natural antioxidants for mitigating weaning stress in piglets. 展开更多
关键词 Antioxidant Growth performance Intestinal flora Nrf2 pathway Piceatannol Weaning piglets
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The gut microbial metabolite indole‑3‑aldehyde alleviates impaired intestinal development by promoting intestinal stem cell expansion in weaned piglets 认领 引用 被引量：6
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作者 Jiaqi Zhang Yahui Chen +5 位作者 Xin Guo Xuan Li Ruofan Zhang Mengting Wang Weiyun Zhu Kaifan Yu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期455-470,共16页
Background Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbio-sis.However,it has been challenging to clarify which specific intestinal microbiota and their metabolites ... Background Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbio-sis.However,it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets.Results In this study,we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score,and elevated levels of lipopolysaccharide(LPS)and D-lactate(D-LA)compared to healthy piglets.Subsequently,we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets.Diarrheal piglets demonstrated intestinal microbiota dysbiosis,characterized pri-marily by a higher Firmicutes to Bacteroidota ratio,a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri,and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron.Functional pro-filing of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes(KEGG)data was performed,and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diar-rhea.Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets.Furthermore,we explored the effects of dietary indole-3-aldehyde(IAld),a key tryptophan metabolite,on intestinal development and gut barrier function in weaned piglets.Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell(ISC)expansion in piglets.The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids.Conclusions These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intesti-nal development by promoting ISC expansion in weaned piglets. 展开更多
关键词 Indole-3-aldehyde Intestinal development Intestinal microbiota Intestinal stem cell Weaned piglets
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Impacts of zinc caproate supplementation on growth performance,intestinal health,anti-inflammatory activity,and Zn homeostasis in weaned piglets challenged with Escherichia coli K88 认领 引用 被引量：2
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作者 Jilong Xu Hanzhen Qiao +7 位作者 Liping Gan Peng Wang Yifeng Zhao Zetian Lei Yixuan Chou Chenrui Hou Mengqi Li Jinrong Wang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第3期1308-1323,共16页
Background Enterotoxigenic Escherichia coli(ETEC)is one of the primary causes of diarrhea in piglets,creating substantial economic losses in the swine farming industry worldwide.This study aimed to investigate the imp... Background Enterotoxigenic Escherichia coli(ETEC)is one of the primary causes of diarrhea in piglets,creating substantial economic losses in the swine farming industry worldwide.This study aimed to investigate the impacts of zinc caproate(ZnCA,C12H22O4Zn)on the intestinal health,growth performance,inflammatory status,and Zn homeostasis of weaned piglets challenged with ETEC K88.In total,48 weaned piglets(Duroc×Landrace×Yorkshire,7.78±0.19 kg,28 d)were selected for a 21-d experiment.Each experimental treatment consisted of 6 replicate pens with 2 piglets each.The treatment conditions were as follows:i)a basal diet(CON),ii)a basal diet+ETEC K88(NC),iii)a basal diet+2,500 mg/kg of Zn(provided as zinc oxide,ZnO)+ETEC K88(PC),and iv)a basal diet+1,600 mg/kg of Zn(provided as ZnCA)+ETEC K88(ZnCA).Results The addition of 1,600 mg/kg ZnCA to the diet of post-weaning piglets effectively enhanced growth performance and nutrient digestibility and reduced the incidence of diarrhea and inflammatory reactions caused by ETEC K88 infection.These therapeutic effects were comparable to those of pharmacological doses of ZnO.In terms of improving intestinal health and Zn homeostasis in post-weaning piglets challenged with ETEC K88,the effectiveness of 1,600 mg/kg ZnCA surpassed that of pharmacological doses of ZnO.Conclusions Overall,under the experimental conditions of this study,ZnCA exhibited the potential to reduce the pharmacological dosage of ZnO while improving intestinal health and Zn homeostasis in weaned piglets. 展开更多
关键词 AGPs ETEC K88 Pharmacological doses of ZnO Weaned pigs ZnCA
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Taurine alleviated paraquat-induced oxidative stress and gut-liver axis damage in weaned piglets by regulating the Nrf2/Keap1 and TLR4/NF-κB signaling pathways 认领 引用
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作者 Chen Chen Min Qi +7 位作者 Weilong Zhang Fanxing Chen Zhihong Sun Weizhong Sun Wenjie Tang Zhenguo Yang Xuan Zhao Zhiru Tang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第6期2852-2874,共23页
Background Oxidative stress can impair intestinal barrier function and cause liver damage,resulting in reduced animal productivity.Paraquat(PQ)induces significant oxidative stress in weaned piglets.The antioxidant,ant... Background Oxidative stress can impair intestinal barrier function and cause liver damage,resulting in reduced animal productivity.Paraquat(PQ)induces significant oxidative stress in weaned piglets.The antioxidant,anti-inflammatory,and metabolic regulatory functions of taurine(Tau),a free amino acid that is widely distributed in the body,have been extensively studied.However,the mechanisms by which dietary Tau alleviates oxidative stress and gut-liver axis damage in weaned piglets remain unclear.Methods Forty weaned piglets(20 males and 20 females;6.41±0.11 kg;25 days old;Duroc×Landrace×Yorkshire)were used in a 2×2 factorial design to investigate the mechanism by which dietary Tau(0%or 0.4%)alleviates PQ-induced oxidative stress and gut-liver axis damage.We analyzed key biomarkers related to gut barrier function,mucosal damage repair,liver damage,gut-liver immunity,antioxidant capacity,systemic immune homeostasis,antioxidant levels,and gut microbiota diversity in piglets under normal and acute oxidative stress.In particular,we evaluated the coordinated regulation of gut-liver axis function mediated by Tau through the Nrf2/Keap1(antioxidant)and TLR4/NF-κB(immune modulation)signaling pathways.Partial least squares path modeling and molecular docking were used to explore the intrinsic relationship between PQ,Tau,and the gut-liver axis.Results PQ exposure impaired gut barrier function,increased the liver fibrosis area,and markedly affected gut microbial diversity(P<0.05).Tau effectively alleviated PQ-induced oxidative stress by activating the Nrf2/Keap1 pathway and inhibiting the TLR4/NF-κB pathway.This enhanced gut barrier function,promoted mucosal repair,and significantly suppressed the concentration and circulation of lipopolysaccharides in the blood,consequently reducing liver damage(P<0.05).This further facilitated the optimization of gut microbiota composition,thereby supporting the positive regulation of the gut-liver axis and improving systemic immune and antioxidant functions.Conclusions Tau improved the health status of weaned piglets under both normal and stressed conditions by modulating the Nrf2/Keap1 and TLR4/NF-κB pathways,offering a potential new nutritional strategy for alleviating gut-liver damage. 展开更多
关键词 Antioxidant Gut-liver axis Immune response Intestinal barrier Lipopolysaccharide Paraquat Taurine Weaned piglets
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Yeast culture in weaned lamb feed:a proteomic journey into enhanced rumen health and growth 认领 引用
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作者 Xueqiang Li Xiaolin Yang +7 位作者 Hui Chen Shixiong Liu Puguo Hao Jie Ning Yingga Wu Xi Liang Yufei Zhang Dacheng Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2389-2416,共28页
Background Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis,enhances performance,and regulates rumen pH.The yeast culture used in this study was developed in-house,and has been show... Background Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis,enhances performance,and regulates rumen pH.The yeast culture used in this study was developed in-house,and has been shown to promote rumen epithelial growth in several sheep trials.Changes in protein expression associated with the promotion of rumen epithelial development following the addition of yeast culture,along with the associated molecular mechanisms,remain unknown.We used 2045-day-old weaned lambs to investigate the specific proteins and molecular mechanisms involved in these processes.Half of the lambs were fed yeast culture,and the other half were used as controls.Results Yeast culture enhanced growth performance,facilitated rumen fermentation,and promoted rumen papilla development in weaned lambs.Proteomics data identified 4,831 proteins in the rumen epithelial tissue of lambs,comprising 87 upregulated and 425 downregulated proteins.Administration of yeast culture activated multiple molecular functions within rumen epithelial cells,including oxidative phosphorylation,glutathione metabolism,apoptosis,cell cycle,and vitamin digestion and absorption.The expression of proteins associated with cell cycle regulation increased,whereas those associated with apoptosis decreased.Administration of yeast culture also reduced the duration of the G0/G1 phase of rumen epithelial cells and accelerated the cell cycle.Furthermore,yeast culture showed increased cyclin D1,cyclin-dependent kinase(CDK)2,CDK4,CDK6,and cyclin E1 expressions and decreased cytochrome C(Cyto-c),Bcl-2-related X protein(Bax),cleaved caspase 3(C-caspase 3),caspase 3,and cleaved caspase 7(C-caspase 7)protein expressions.Yeast culture upregulated the insulin-like growth factor-1 receptor(IGF-1R)and insulin-like growth factor-binding protein 5(IGFBP-5)mRNA expressions in rumen epithelial cells.Conclusions Yeast culture facilitates rumen epithelial development by regulating the cell cycle and IGF-1 signaling and reducing the expression of proteins associated with apoptosis in rumen epithelial cells.The findings of this study provide novel insights into the molecular mechanisms through which yeast culture promotes rumen epithelial development in weaned lambs. 展开更多
关键词 Apoptosis Cell cycle Proteome Rumen epithelial Weaned lamb Yeast culture
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Effects of monoglyceride blend on systemic and intestinal immune responses,and gut health of weaned pigs experimentally infected with a pathogenic Escherichia coli 认领 引用 被引量：3
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作者 Sangwoo Park Shuhan Sun +5 位作者 Lauren Kovanda Adebayo O.Sokale Adriana Barri Kwangwook Kim Xunde Li Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期345-363,共19页
Background Monoglycerides have emerged as a promising alternative to conventional practices due to their biolog-ical activities,including antimicrobial properties.However,few studies have assessed the efficacy of mono... Background Monoglycerides have emerged as a promising alternative to conventional practices due to their biolog-ical activities,including antimicrobial properties.However,few studies have assessed the efficacy of monoglyceride blend on weaned pigs and their impacts on performance,immune response,and gut health using a disease chal-lenge model.Therefore,this study aimed to investigate the effects of dietary monoglycerides of short-and medium-chain fatty acids on the immunity and gut health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli F18.Results Pigs supplemented with high-dose zinc oxide(ZNO)had greater(P<0.05)growth performance than other treatments,but no difference was observed in average daily feed intake between ZNO and monoglycerides groups during the post-challenge period.Pigs in ZNO and antibiotic groups had lower(P<0.05)severity of diarrhea than control,but the severity of diarrhea was not different between antibiotic and monoglycerides groups.Pigs fed with monoglycerides or ZNO had lower(P<0.05)serum haptoglobin on d 2 or 5 post-inoculation than control.Pigs in ZNO had greater(P<0.05)goblet cell numbers per villus,villus area and height,and villus height:crypt depth ratio(VH:CD)in duodenum on d 5 post-inoculation than pigs in other treatments.Pigs supplemented with monoglyc-erides,ZNO,or antibiotics had reduced(P<0.05)ileal crypt depth compared with control on d 5 post-inoculation,contributing to the increase(P=0.06)in VH:CD.Consistently,pigs in ZNO expressed the lowest(P<0.05)TNFa,IL6,IL10,IL12,IL1A,IL1B,and PTGS2 in ileal mucosa on d 5 post-inoculation,and no difference was observed in the expres-sion of those genes between ZNO and monoglycerides.Supplementation of ZNO and antibiotic had significant impacts on metabolic pathways in the serum compared with control,particularly on carbohydrate and amino acid metabolism,while limited impacts on serum metabolites were observed in monoglycerides group when compared with control.Conclusions The results suggest that supplementation of monoglyceride blend may enhance disease resist-ance of weaned pigs by alleviating the severity of diarrhea and mitigating intestinal and systemic inflammation,although the effectiveness may not be comparable to high-dose zinc oxide. 展开更多
关键词 Diarrhea Enterotoxigenic Escherichia coli Gut health Monoglycerides Systemic immunity Weaned pigs
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Dietary supplementation of blend of organic acids and monoglycerides alleviated diarrhea and systemic inflammation of weaned pigs experimentally infected with enterotoxigenic Escherichia coli F18 认领 引用 被引量：2
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作者 Sangwoo Park Shuhan Sun +3 位作者 Supatirada Wongchanla Ying Chen Xunde Li Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第3期1255-1268,共14页
Background The emergence of antibiotic resistant microorganisms associated with conventional swine production practices has increased interest in acid-based compounds having antimicrobial properties and other biologic... Background The emergence of antibiotic resistant microorganisms associated with conventional swine production practices has increased interest in acid-based compounds having antimicrobial properties and other biological functions as nutritional interventions.Despite the interest in organic acids and monoglycerides,few studies have examined the effects of the combination of these acid-based additives in weaned pigs under disease challenge conditions.Therefore,this study aimed to investigate the effects of dietary supplementation with blend of organic acids and/or medium-chain fatty acid monoglycerides on intestinal health and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli(ETEC)F18 at 4-week of age.Results Dietary supplementation of organic acids,monoglycerides,or both organic acids and monoglycerides(combination)reduced(P<0.05)the diarrhea frequency of ETEC F18-infected pigs throughout the experimental period(d−7 to 21 post-inoculation).This is consistent with the reduced(P<0.05)proportion ofβ-hemolytic coliforms in feces observed for the organic acid and combination treatments on d 10 post-inoculation.Supplementation of organic acids,monoglycerides,or combination also reduced(P<0.05)bacterial translocation in mesenteric lymph nodes on d 21 post-inoculation.Pigs fed with monoglycerides or combination had lower(P<0.05)white blood cells on d 5 post-inoculation,and pigs fed the combination also had lower(P<0.05)lymphocytes than pigs in control group.Monoglyceride supplementation increased(P<0.05)white blood cells and neutrophils compared with control group on d 14 post-inoculation.However,supplementation with organic acid blend,monoglyceride blend,or combination did not affect growth performance in this experiment.Conclusions Supplementation with monoglycerides or organic acids alone or in combination improves the detrimental effects of ETEC F18 infection in weaned pigs,as indicated by reduced diarrhea,fecal shedding ofβ-hemolytic coliforms,and bacterial translocation,and thus enhancing disease resistance.Monoglycerides reduced the inflammatory response during peak infection,but their immunomodulatory and possible synergistic effects with organic acids need to be further investigated. 展开更多
关键词 Acidifiers Antimicrobial agents Diarrhea Enterotoxigenic Escherichia coli Monoglycerides Systemic immunity Weaned pigs
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Effects of dietary L-glutamate and L-aspartate supplementation on growth performance,severity of diarrhea,intestinal barrier integrity,and fecal microbiota of weaned piglets challenged with F18 enterotoxigenic Escherichia coli 认领 引用 被引量：1
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作者 Supatirada Wongchanla Kunal Dixit +9 位作者 Sangwoo Park Kwangwook Kim Shuhan Sun Maria Marco Steven B.Palomares Alejandra Mejia-Caballero Sahana Mohan Xunde Li Xiaojing Li Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第6期2895-2909,共15页
Background L-Glutamate and L-aspartate are functional amino acids that play pivotal roles in the cellular metabolic pathways of swine enterocytes.Therefore,this study aimed to investigate the effects of dietary L-glut... Background L-Glutamate and L-aspartate are functional amino acids that play pivotal roles in the cellular metabolic pathways of swine enterocytes.Therefore,this study aimed to investigate the effects of dietary L-glutamate and L-aspartate on growth performance,diarrhea severity,intestinal barrier integrity,and fecal microbiota of weaned piglets challenged with F18 enterotoxigenic Escherichia coli(ETEC).Weaned piglets were randomly assigned to seven dietary treatments,including unchallenged and ETEC-challenged controls,amino acid-supplemented groups,and an antibiotic control,to assess their responses to ETEC challenge.Results Supplementation with 1%L-glutamate or 2%L-aspartate enhanced growth performance,with significantly greater(P<0.05)average daily weight gain and gain-to-feed ratio compared with the positive control group from d 0 to d 5 post-inoculation.Pigs fed with 1%or 2%L-aspartate had reduced(P<0.05)diarrhea severity in ETEC-challenged pigs compared with the positive control group.The 1%L-aspartate supplementation also supported intestinal structure by increasing(P<0.05)duodenal villi height and ileal villi width compared with carbadox supplementation.Additionally,1%L-glutamate supplementation significantly improved(P<0.05)resilience in ETEC-challenged pigs by reducing fecal shedding ofβ-hemolysin-producing bacteria compared with the positive control group on d 14 post-inoculation.Moreover,1%L-aspartate supplementation promoted intestinal barrier integrity by significantly up-regulated(P<0.05)the expression of ileal OCDN and ileal ZO-1 compared with the positive control group on d 14 post-inoculation.Interestingly,2%L-aspartate supplementation altered the intestinal mucosa by down-regulating(P<0.05)the expression of jejunal CLDN-1,while up-regulating(P<0.05)the expression of ileal CLDN-1 compared with the negative control group on d 14 post-inoculation.Furthermore,L-glutamate supplementation significantly changed proportions of Firmicutes and Bacteroidota and showed the trend for enrichment in beneficial bacterial genera such as Bifidobacterium and Megasphaera in ETEC-infected pigs by d 14 post-inoculation.Conclusion Supplementation with L-glutamate or L-aspartate promoted growth performance,supported gut health,and enhanced disease resistance in weaned pigs challenged with F18 ETEC.During the weaning period,L-glutamate or L-aspartate could potentially be considered conditionally essential amino acids,helping to alleviate weaning complications and reduce the need for antibiotic use in swine farming. 展开更多
关键词 Diarrhea Escherichia coli Fecal microbiota L-Aspartate L-Glutamate Weaned pigs
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Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets 认领 引用 被引量：23
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作者 Jie Yu Yanyan Song +10 位作者 Bing Yu Jun He Ping Zheng Xiangbing Mao Zhiqing Huang Yuheng Luo Junqiu Luo Hui Yan Quyuan Wang Huifen Wang Daiwen Chen 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第2期700-710,共11页
Background:Tannic acid(TA)is potential to reduce diarrhea in weaning pigs,but knowledge about the influence of TA on intestinal barrier integrity and function is still scarce.This experiment was conducted to investiga... Background:Tannic acid(TA)is potential to reduce diarrhea in weaning pigs,but knowledge about the influence of TA on intestinal barrier integrity and function is still scarce.This experiment was conducted to investigate the effects of dietary TA supplementation on growth performance,diarrhea rate,intestinal barrier integrity and function of weaned pigs.Methods:A total of 108 crossbred(Duroc×Landrace×Yorkshire)piglets,with an initial average body weight of 6.60±0.27 kg,were allotted to 3 groups(6 pigs/pen and 6 replicates/group)in a randomized complete block design according to their gender and body weight.Piglets were fed the basal diet with 0(control,CON),0.2%and 1.0%TA,respectively.The trial lasted for 28 d.Results:Compared with the CON group,dietary 0.2%and 1.0%TA supplementation didn’t affect ADFI,ADG and F:G(P>0.05),but reduced diarrhea rate,diarrhea index and diarrhea score of piglets(P<0.05),reduced diamine oxidase(DAO)activity and D-lactic acid concentration in serum(P<0.01).The higher occludin expression and localization were observed in the duodenum,jejunum and ileum after supplementation with 0.2%or 1.0%TA(P<0.05).Adding 0.2%TA to diet significantly decreased crypt depth,increased villus height/crypt depth ratio in the duodenum(P<0.05),and dietary 1.0%TA tended to decrease crypt depth(P<0.10)and significantly decreased villus height(P<0.05)of the ileum.Moreover,lower malondialdehyde content in the ileum was detected in the pigs fed 1.0%TA(P<0.05).In the duodenum,both 0.2%and 1.0%TA groups had higher occludin(OCLN)mRNA and 0.2%TA group had higher zonula occludens-2(ZO-2)level(P<0.05).Meanwhile,dietary 1.0%TA supplementation tended to up-regulate OCLN mRNA levels in the jejunum(P<0.10)and 0.2%TA supplementation tended to up-regulate zonula occludens-1(ZO-1)mRNA levels in the ileum(P<0.10).Conclusion:In conclusion,dietary supplementation of 0.2%or 1.0%TA could effectively alleviate postweaning diarrhea without altering growth performance in weaned piglets,which might be achieved by improving intestinal barrier integrity and function. 展开更多
关键词 Intestine barrier Post-weaning diarrhea Tannic acid Weaned piglets
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Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity 认领 引用 被引量：20
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作者 Xiangqi Fan Haiyan Hu +9 位作者 Daiwen Chen Bing Yu Jun He Jie Yu Junqiu Luo Erik Eckhardt Yuheng Luo Jianping Wang Hui Yan Xiangbing Mao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第3期1125-1137,共13页
Background:Lentinan(LNT)may regulate many important physiological functions of human and animals.This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)... Background:Lentinan(LNT)may regulate many important physiological functions of human and animals.This study aimed to verify whether LNT administration could relieve diarrhea via improving gut immunity in rotavirus(RV)-challenged weaned pigs.Methods:Twenty-eight weaned pigs were randomly fed 2 diets containing 0 or 84 mg/kg LNT product for 19 d(n=14).RV infection was executed on d 15.After extracting polysaccharides from LNT product,its major monosaccharides were analyzed.Then,LNT polysaccharide was used to administrate RV-infected IPEC-J2 cells.Results:Dietary LNT supplementation supported normal function of piglets even when infected with RV,as reflected by reduced growth performance loss and diarrhea prevalence,and maintained gut immunity(P<0.05).The polysaccharide was isolated from LNT product,which molecular weight was 5303 Da,and major monosaccharides included glucose,arabinose and galactose.In RV-infected IPEC-J2 cells,this polysaccharide significantly increased cell viability(P<0.05),and significantly increased anti-virus immunity via regulating pattern recognition receptors and host defense peptides(P<0.05).Conclusion:Those results suggest that LNT administration increases the piglets’resistance to RV-induced stress,likely by supporting intestinal immunity. 展开更多
关键词 Gut immunity IPEC-J2 cells Lentinan Rotavirus Weaned pigs
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Effects of yeast products on the intestinal morphology,barrier function,cytokine expression,and antioxidant system of weaned piglets 认领 引用 被引量：18
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作者 Huan-sheng YANG Fei WU +5 位作者 Li-na LONG Tie-jun LI Xia XIONG Peng LIAO Hong-nan LIU Yu-long YIN 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2016年第10期752-762,共11页
The goal of this study was to evaluate the effects of a mixture of yeast culture,cell wall hydrolysates,and yeast extracts(collectively"yeast products,"YP)on the performance,intestinal physiology,and health ... The goal of this study was to evaluate the effects of a mixture of yeast culture,cell wall hydrolysates,and yeast extracts(collectively"yeast products,"YP)on the performance,intestinal physiology,and health of weaned piglets.A total of 90 piglets weaned at 21 d of age were blocked by body weight,sex,and litter and randomly assigned to one of three treatments for a 14-d feeding experiment,including(1)a basal diet(control),(2)1.2 g/kg of YP,and(3)20 mg/kg of colistin sulfate(CSE).No statistically significant differences were observed in average daily feed intake,average daily weight gain,or gain-to-feed ratio among CSE,YP,and control piglets.Increased prevalence of diarrhea was observed among piglets fed the YP diet,whereas diarrhea was less prevalent among those fed CSE.Duodenal and jejunal villus height and duodenal crypt depth were greater in the control group than they were in the YP or CSE groups.Intraepithelial lymphocytes(IEL)in the duodenal and jejunal villi were enhanced by YP,whereas IEL in the ileal villi were reduced in weaned piglets fed YP.Secretion of jejunal and ileal interleukin-10(IL-10)was higher and intestinal and serum antioxidant indexes were affected by YP and CSE.In YP-and CSE-supplemented animals,serum D-lactate concentration and diamine oxidase(DAO)activity were both increased,and intestinal mR NA expressions of occludin and ZO-1 were reduced as compared to the control animals.In conclusion,YP supplementation in the diets of weaned piglets appears to increase the incidence of diarrhea and has adverse effects on intestinal morphology and barrier function. 展开更多
关键词 Yeast products Weaned piglet Intestinal morphology Barrier function Antioxidant system
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Alginate oligosaccharide-induced intestinal morphology, barrier function and epithelium apoptosis modifications have beneficial effects on the growth performance of weaned pigs 认领 引用 被引量：17
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作者 Jin Wan Jiao Zhang +6 位作者 Daiwen Chen Bing Yu Xiangbing Mao Ping Zheng Jie Yu Junqiu Luo Jun He 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2018年第4期937-948,共12页
Background: Alginate oligosaccharide(AOS), produced from alginate by alginate lyase-mediated depolymerisation, is a potential substitute for antibiotics and possesses growth-enhancing effects. Nevertheless, the mechan... Background: Alginate oligosaccharide(AOS), produced from alginate by alginate lyase-mediated depolymerisation, is a potential substitute for antibiotics and possesses growth-enhancing effects. Nevertheless, the mechanisms by which AOS regulates porcine growth remain to be elucidated. Therefore, we investigated the AOS-mediated changes in the growth performance of weaned pigs by determining the intestinal morphology, barrier function,as well as epithelium apoptosis.Methods: Twenty-four weaned pigs were distributed into two groups(n = 12) and received either a basal diet(control group) or the same diet supplemented with 100 mg/kg AOS. On d 15, D-xylose(0.1 g/kg body weight)was orally administrated to eight randomly selected pigs per treatment, and their serum and intestinal mucosa samples were collected 1 h later.Results: Our results showed that inclusion of AOS in the diet for 2 wk increased(P < 0.05) the average daily body weight gain in weaned pigs. Notably, AOS supplementation ameliorated the intestinal morphology and barrier function, as suggested by the enhanced(P < 0.05) intestinal villus height, secretory immunoglobulin A content and goblet cell counts. Compared to the control group, AOS ingestion both decreased(P < 0.05) the total apoptotic percentage and increased(P < 0.05) the proportion of S phase in the intestinal epithelial cells. Furthermore, AOS not only up-regulated(P < 0.05) the B-cell lymphoma-2(BCL2) transcriptional level but also down-regulated(P < 0.05) the B-cell lymphoma-2-associated X protein(BAX), cysteinyl aspartate-specific proteinase-3(caspase-3) and caspase-9 transcriptional levels in the small intestine.Conclusions: In summary, this study provides evidence that supplemental AOS beneficially affects the growth performance of weaned pigs, which may result from the improved intestinal morphology and barrier function,as well as the inhibited enterocyte death, through reducing apoptosis via mitochondria-dependent apoptosis. 展开更多
关键词 Alginate oligosaccharide Barrier function Cell apoptosis Intestinal morphology Weaned pigs
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The effect of dietary tryptophan levels on oxidative stress of liver induced by diquat in weaned piglets 认领 引用 被引量：22
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作者 Xiangbing Mao Mei Lv +5 位作者 Bing Yu Jun He Ping Zheng Jie Yu Quyuan Wang Daiwen Chen 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2015年第1期104-110,共7页
Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diq... Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat. A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18, 0.30, and 0A5% for 14 d. On day 8, the piglets were injected intraperitoneally with sterile 0.9% NaCI solution or diquat （10 mg/kg body weight）. During the first 7 d of trial, increasing dietary tryptophan levels enhanced average daily gain （P = 0.09） and average daily feed intake （P = 0.08）, and decreased the feed efficiency （P 〈 0.05） of piglets. The growth performance was decreased by diquat injection （P 〈 0.05）. Diquat injection also decreased the activities of the superoxide dismutase （SOD） and glutathione peroxidase （GPx） in the plasma and liver （P 〈 0.05）, increased plasma malondialdehyde （MDA） （P 〈 0.05） and urea nitrogen （P 〈 0.05） concentrations, and enhanced MDA concentration （P = 0.09） and tryptophan 2,3-dioxygenase （TDO） activity （P = 0.07） in liver of piglets. Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA （P = 0.06） concentration and the activities of SOD （P = 0.09） and GPx （P = 0.05） of the liver, and plasma urea nitrogen （P = 0.06） concentration in the piglet. There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection （P 〈 0.05）. These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity. 展开更多
关键词 Antioxidant capacity Diquat Oxidative stress of livers Tryptophan,Weaned piglets
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