表达副猪格拉瑟菌GAPDH和OmP26的重组猪霍乱沙门氏菌构建及免疫学评价认领

Construction and immunological evaluation of recombinant Salmonella choleraesuis expressing GAPDH and OmP26 of Glaesserella parasuis

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摘要【目的】构建能同时表达副猪格拉瑟菌Glaesserella parasuis关键抗原GAPDH和OmP26的重组猪霍乱沙门氏菌Salmonella choleraesuis,并评估其作为二联疫苗候选株的潜力,以期为预防G...展开更多【Objective】The objective of this study was to construct a recombinant Salmonella choleraesuis strain co-expressing GAPDH and OmP26,two immunogenic antigens derived from Glaesserella parasuis,evaluate its potential as a bivalent vaccine candidate,and provide a novel and ...MORE 【Objective】The objective of this study was to construct a recombinant Salmonella choleraesuis strain co-expressing GAPDH and OmP26,two immunogenic antigens derived from Glaesserella parasuis,evaluate its potential as a bivalent vaccine candidate,and provide a novel and efficacious solution for the prevention of both G.parasuis and S.choleraesuis infections in swine populations.【Method】GAPDH and OmP26,which are widely present in various serotypes of G.parasuis,were selected as exogenous antigens.A recombinant strain of G.parasuis-S.choleraesuis,which was capable of expressing both the GAPDH and OmP26,was constructed by using S.choleraesuis C500Δasd deletion strain as vector.The biological characteristic and immune effect of the recombinant strain were then investigated.【Result】Results of PCR and Sanger-sequencing showed that we successfully constructed the recombinant strain C501(pYA-GAPDH-OmP26),which was able to stably harbor GAPDH and OmP26(sizes of 1020 and 798 bp,respectively).The target fragments were stably amplified from the strain in 100 consecutive passages,and the recombinant strain was consistent with the parent strain C501(pYA3493)in terms of growth curves and biochemical characteristics.The recombinant strain C501(pYA-GAPDH-OmP26)showed 62.5%and 50.0%protection rates against S.choleraesuis C78-1 and G.parasuis type 5 strong strain SH0165,respectively,while C501(pYA3493)showed 50.0%protection rate against S.choleraesuis C78-1 and no protection effect against G.parasuis type 5 strong strain SH0165.【Conclusion】The recombinant S.choleraesuis C501(pYA-GAPDH-OmP26)can stably carry heterologous genes.Compared with the parent strain,the recombinant strain has similar biological and good expression characteristics,can induce the organism’s combined immune response against G.parasuis and S.choleraesuis.The study lays the foundation for the development of the bivalent genetically engineered vaccine for G.parasuis and S.choleraesuis.FEWER

作者陈丽璇符颖陈政权李莉莉张伟孝张建民 CHEN Lixuan;FU Ying;CHEN Zhengquan;LI Lili;ZHANG Weixiao;ZHANG Jianmin(National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control/Key Lab for Zoonosis of Ministry of Agriculture and Rural Affairs/Key Laboratory of Animal Vaccine Development of Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Zoonotic Diseases of Animal Origin/College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China)

机构地区人兽共患病防控制剂国家地方联合工程实验室/农业农村部人兽共患病重点实验室/农业农村部兽用疫苗创制重点实验室/广东省动物源性人兽共患病重点实验室/华南农业大学兽医学院

出处《华南农业大学学报》 CAS CSCD 北大核心 2025年第3期319-325,共7页 Journal of South China Agricultural University

基金 “十四五”国家重点研发计划(2022YFD1800901)。

关键词猪副猪格拉瑟菌猪霍乱沙门氏菌 C500Δasd缺失株 GAPDH OmP26 Pig Glaesserella parasuis Salmonella choleraesuis C500Δasd deletion strain GAPDH OmP26

分类号 S852.61 [农业科学—基础兽医学]